Scientific Reports最新文献_第7页

miR-1, miR-133a, miR-29b and skeletal muscle fibrosis in chronic limb-threatening ischaemia. miR-1、miR-133a、miR-29b 与慢性肢体缺血的骨骼肌纤维化。

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-11-26 DOI: 10.1038/s41598-024-76415-9

Alan J Keane, Clara Sanz-Nogués, Dulan Jayasooriya, Michael Creane, Xizhe Chen, Caomhán J Lyons, Isha Sikri, Katarzyna Goljanek-Whysall, Timothy O'Brien

{"title":"miR-1, miR-133a, miR-29b and skeletal muscle fibrosis in chronic limb-threatening ischaemia.","authors":"Alan J Keane, Clara Sanz-Nogués, Dulan Jayasooriya, Michael Creane, Xizhe Chen, Caomhán J Lyons, Isha Sikri, Katarzyna Goljanek-Whysall, Timothy O'Brien","doi":"10.1038/s41598-024-76415-9","DOIUrl":"https://doi.org/10.1038/s41598-024-76415-9","url":null,"abstract":"Chronic limb-threatening ischaemia (CLTI), the most severe manifestation of peripheral arterial disease (PAD), is associated with a poor prognosis and high amputation rates. Despite novel therapeutic approaches being investigated, no significant clinical benefits have been observed yet. Understanding the molecular pathways of skeletal muscle dysfunction in CLTI is crucial for designing successful treatments. This study aimed to identify miRNAs dysregulated in muscle biopsies from PAD cohorts. Using MIcroRNA ENrichment TURned NETwork (MIENTURNET) on a publicly accessible RNA-sequencing dataset of PAD cohorts, we identified a list of miRNAs that were over-represented among the upregulated differentially expressed genes (DEGs) in CLTI. Next, we validated the altered expression of these miRNAs and their targets in mice with hindlimb ischaemia (HLI). Our results showed a significant downregulation of miR-1, miR-133a, and miR-29b levels in the ischaemic limbs versus the contralateral non-ischaemic limb. A miRNA target protein-protein interaction network identified extracellular matrix components, including collagen-1a1, -3a1, and -4a1, fibronectin-1, fibrin-1, matrix metalloproteinase-2 and -14, and Sparc, which were upregulated in the ischaemic muscle of mice. This is the first study to identify miR-1, miR-133a, and miR-29b as potential contributors to fibrosis and vascular pathology in CLTI muscle, which supports their potential as novel therapeutic agents for this condition.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"29393"},"PeriodicalIF":3.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclic electron flow compensates loss of PGDH3 and concomitant stromal NADH reduction. 循环电子流可补偿 PGDH3 的损失和随之而来的基质 NADH 减少。

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-11-26 DOI: 10.1038/s41598-024-80836-x

Moritz Krämer, Nicolás E Blanco, Jan-Ferdinand Penzler, Geoffry A Davis, Benjamin Brandt, Dario Leister, Hans-Henning Kunz

{"title":"Cyclic electron flow compensates loss of PGDH3 and concomitant stromal NADH reduction.","authors":"Moritz Krämer, Nicolás E Blanco, Jan-Ferdinand Penzler, Geoffry A Davis, Benjamin Brandt, Dario Leister, Hans-Henning Kunz","doi":"10.1038/s41598-024-80836-x","DOIUrl":"10.1038/s41598-024-80836-x","url":null,"abstract":"In nature plants constantly experience changes in light intensities. Low illumination limits photosynthesis and growth. However, also high light intensities are a threat to plants as the photosynthetic machinery gets damaged when the incoming energy surpasses the capacity of photochemistry. One limitation of photochemistry is the constant resupply of stromal electron (e-) acceptors, mainly NADP. NADP is reduced at the acceptor-side of photosystem I. The resulting NADPH is utilized by the Calvin-Benson-Bassham cycle (CBBC) and the malate valve to ensure sufficient oxidized NADP ready to accept e- from PSI. Lately, additional pathways, which function as stromal e- sinks under abiotic stress conditions, were discovered. One such reaction in Arabidopsis thaliana is catalyzed by PHOSPHOGLYCERATE DEHYDROGENASE 3 (PGDH3), which diverts e- from the CBBC into NADH. pgdh3 loss-of-function mutants exhibit elevated non-photochemical quenching (NPQ) and fluctuating light susceptibility. To optimize plant photosynthesis in challenging environments knowledge on PGDH3's metabolic integration is needed. We used the source of high NPQ in pgdh3 as a starting point. Our study reveals that increased NPQ originates from high cyclic electron flow (CEF). Interestingly, PGDH3 function seems very important when the CEF-generator PROTON GRADIENT REGULATION5 (PGR5) is lost. Consequently, pgr5pgdh3 double mutants are more sensitive to fluctuating light.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"29274"},"PeriodicalIF":3.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gucy1α1 specifically marks kidney, heart, lung and liver fibroblasts. Gucy1α1 能特异性地标记肾、心、肺和肝成纤维细胞。

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-11-26 DOI: 10.1038/s41598-024-80930-0

Valeria Rudman-Melnick, Davy Vanhoutte, Kaitlynn Stowers, Michelle Sargent, Mike Adam, Qing Ma, Anne Karina T Perl, Alexander G Miethke, Ashley Burg, Tiffany Shi, David A Hildeman, E Steve S Woodle, J Matthew Kofron, Prasad Devarajan

{"title":"Gucy1α1 specifically marks kidney, heart, lung and liver fibroblasts.","authors":"Valeria Rudman-Melnick, Davy Vanhoutte, Kaitlynn Stowers, Michelle Sargent, Mike Adam, Qing Ma, Anne Karina T Perl, Alexander G Miethke, Ashley Burg, Tiffany Shi, David A Hildeman, E Steve S Woodle, J Matthew Kofron, Prasad Devarajan","doi":"10.1038/s41598-024-80930-0","DOIUrl":"https://doi.org/10.1038/s41598-024-80930-0","url":null,"abstract":"Fibrosis is a common outcome of numerous pathologies, including chronic kidney disease (CKD), a progressive renal function deterioration. Current approaches to target activated fibroblasts, key effector contributors to fibrotic tissue remodeling, lack specificity. Here, we report Gucy1α1 as a specific kidney fibroblast marker. Gucy1α1 levels significantly increased over the course of two clinically relevant murine CKD models and directly correlated with established fibrosis markers. Immunofluorescent (IF) imaging showed that Gucy1α1 comprehensively labelled cortical and medullary quiescent and activated fibroblasts in the control kidney and throughout injury progression, respectively. Unlike traditionally used markers platelet derived growth factor receptor beta (Pdgfrβ) and vimentin (Vim), Gucy1α1 did not overlap with off-target populations such as podocytes. Notably, Gucy1α1 labelled kidney fibroblasts in both male and female mice. Furthermore, we observed elevated GUCY1α1 expression in the human fibrotic kidney and lung. Studies in the murine models of cardiac and liver fibrosis revealed Gucy1α1 elevation in activated Pdgfrβ-, Vim- and alpha smooth muscle actin (αSma)-expressing fibroblasts paralleling injury progression and resolution. Overall, we demonstrate Gucy1α1 as an exclusive fibroblast marker in both sexes. Due to its multiorgan translational potential, GUCY1α1 might provide a novel promising strategy to specifically target and mechanistically examine fibroblasts.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"29307"},"PeriodicalIF":3.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mining differentially expressed genes during paratomy in the transcriptome of the flatworm Stenostomum leucops. 在扁形蠕虫 Stenostomum leucops 的转录组中挖掘解剖过程中的差异表达基因。

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-11-26 DOI: 10.1038/s41598-024-80881-6

Marcos Trindade da Rosa, Elgion Lucio Silva Loreto

{"title":"Mining differentially expressed genes during paratomy in the transcriptome of the flatworm Stenostomum leucops.","authors":"Marcos Trindade da Rosa, Elgion Lucio Silva Loreto","doi":"10.1038/s41598-024-80881-6","DOIUrl":"10.1038/s41598-024-80881-6","url":null,"abstract":"Paratomy is an asexual reproductive process that occurs in annelids, flatworms and other groups. During this process, anterior structures are formed in the middle of the body, giving rise to segmented organisms, each segment being called a zooid. Once formed, the zooids detach and form new organisms. Using RNAseq of worms prior to zooid formation and with two zooids, we search for genes that are differentially expressed and may be related to the control of this process in the flatworm Stenostomum leucops. Several signaling pathways showed differential expression, including MAPK/ERK, PI3K-Akt, Wnt, TGFb, mTOR, FoxO and others. Forty-five genes were found to be particularly significant because they are differentially expressed and play an important role in the development of other flatworms. These include ERK, MKP, JNK, PI3K, PTEN, β -catenin, FoxO, Sufu, GH and others. The results suggest some similarities in gene regulation between paratomy and regeneration observed in other flatworms after fission or amputation. In worms without zooids, pathways required for cell proliferation, differentiation of cells into multiple cell lineages and determination of the body axis are activated. In worms with 2 zooids, genes involved in cell growth and apoptosis are activated. Activation of genes involved in neoblast proliferation and maintenance appears to occur at both stages.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"29267"},"PeriodicalIF":3.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DFT study of the adsorption properties and sensitivity of a B₂N monolayer toward harmful gases. 关于 B2N 单层对有害气体的吸附特性和敏感性的 DFT 研究。

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-11-26 DOI: 10.1038/s41598-024-77659-1

Rezvan Rahimi, Mohammad Solimannejad, Ashkan Horri

{"title":"DFT study of the adsorption properties and sensitivity of a B2N monolayer toward harmful gases.","authors":"Rezvan Rahimi, Mohammad Solimannejad, Ashkan Horri","doi":"10.1038/s41598-024-77659-1","DOIUrl":"https://doi.org/10.1038/s41598-024-77659-1","url":null,"abstract":"In this study, we investigate the adsorption of harmful gases - CO, NO, NO2, SO2, and O3 molecules - on a B2N monolayer using periodic density functional theory. The adsorption energy values for the CO/B2N, NO/B2N, NO2/B2N, SO2/B2N, and O3/B2N complexes are determined to be -1.96, -1.39, -1.80, -0.70, and - 2.36 eV, respectively. The B2N monolayer has the ability to adsorb harmful gas molecules, even in humid air, and displays favorable adsorption energy and standard recovery time when exposed to SO2 gas. Consequently, the impact of SO2 gases on the transmission characteristics of the B2N monolayer has been assessed through current-voltage analysis. These findings are of great importance as they serve to demonstrate the remarkable sensing capabilities of a B2N monolayer in efficiently detecting SO2 gas. The desorption time for CO, NO, NO2, and O3 molecules is quite long, thereby indicating the remarkable stability of the B2N sheet for adsorption of these gases. The current study offer valuable insights for further research into the potential utilization of B2N monolayers in long-term monitoring and gas purification applications, specifically in relation to four toxic gases: CO, NO, NO2, and O3.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"29282"},"PeriodicalIF":3.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microsphere-Augmented PDMS integration in tapered FBG small-scale sensors for enhanced temperature sensitivity. 在锥形 FBG 小型传感器中集成微球增强型 PDMS，以提高温度灵敏度。

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-11-26 DOI: 10.1038/s41598-024-80781-9

Bryan Sanipatin, Luis A Sánchez, Lucía Arqués, Salvador Sales

{"title":"Microsphere-Augmented PDMS integration in tapered FBG small-scale sensors for enhanced temperature sensitivity.","authors":"Bryan Sanipatin, Luis A Sánchez, Lucía Arqués, Salvador Sales","doi":"10.1038/s41598-024-80781-9","DOIUrl":"https://doi.org/10.1038/s41598-024-80781-9","url":null,"abstract":"This work presents a novel high-sensitivity temperature sensor based on a fiber Bragg grating inscribed on a tapered optical fiber which terminates in a microsphere, all embedded within a PDMS-filled silica capillary. The fabricated microsphere at the taper's end enhances PDMS traction, improving strain transfer between the polymer and the fiber during temperature changes. Different waist diameters for the tapered fiber were considered for the design of the sensor. The sensor's response was analyzed over a temperature range of 20 °C to 90 °C for taper waist diameters ranging from 60 μm to 20 μm. Experimental results demonstrate a wavelength temperature sensitivity of 47.19 pm °C⁻1 for a 60 μm waist diameter and 221.2 pm °C⁻1 for a 20 μm waist diameter, achieving up to 22 times the sensitivity of a bare FBG. The experimental results were supported by finite element analysis simulations, which showed a clear correlation between the enhanced sensitivity and the increase in axial strain applied by the PDMS on the embedded fiber. This enhancement in sensitivity was demonstrated by housing the fiber in a capillary, integrating the microsphere at the end of the fiber, and diminishing the fiber taper's diameter. Moreover, unlike traditional techniques aimed at enhancing the thermal sensitivity of fiber Bragg gratings, the sensor developed through this innovative approach exhibits enhanced performance regarding both dimensions and sensitivity.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"29376"},"PeriodicalIF":3.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on geological hazard characteristics and susceptibility of the Duku Highway based on SBAS-InSAR and improved spatiotemporal clustering. 基于 SBAS-InSAR 和改进的时空聚类的 Duku 公路地质灾害特征和易发性研究。

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-11-26 DOI: 10.1038/s41598-024-80286-5

Yaxuan Niu, Yan Xu, Chenyu Guo, Jie Liu, Jiangpeng Zhang, Qi Liu, Zhiwei Yang, Jun Zhang

{"title":"Research on geological hazard characteristics and susceptibility of the Duku Highway based on SBAS-InSAR and improved spatiotemporal clustering.","authors":"Yaxuan Niu, Yan Xu, Chenyu Guo, Jie Liu, Jiangpeng Zhang, Qi Liu, Zhiwei Yang, Jun Zhang","doi":"10.1038/s41598-024-80286-5","DOIUrl":"https://doi.org/10.1038/s41598-024-80286-5","url":null,"abstract":"The high-altitude Duku Highway is characterized by complex terrain changes and frequent geological hazards, which severely impact the lives of local residents and the sustainable development of the regional economy. The lack of understanding of terrain deformation, coupled with scarce foundational observation data, makes it challenging to apply mainstream susceptibility assessment methods such as slope modeling and causality analysis. Consequently, this study utilizes Sentinel-1 A data and employs the SBAS-InSAR technique to extract and analyze the deformation characteristics of 184 hazard areas along the Duku Highway over nearly three years. Furthermore, the Correlation Clustering Evaluation Model is proposed, attributing hazard properties to unsupervised spatial clustering results, thus enabling the study of hazard susceptibility in data-scarce regions without prior knowledge. The results indicate that the SBAS-InSAR coherence is 0.64, with a validation accuracy of 85%. The high, relatively-high, and moderate susceptibility areas account for 24.7%, 17.1%, and 32.6% of the total area, respectively. The rapid uplift of terrain due to plate compression is a major factor leading to frequent hazards in high and relatively-high susceptibility areas. These regions may spontaneously experience cyclic hazards (minimum of 2 months) without extreme external factors. The research findings offer new insights into regional hazards and provide a basis for the sustainable management of highways.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"29323"},"PeriodicalIF":3.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of recycled battery graphite decorated with poly hippuric acid/ multiwalled carbon nanotubes as an ecofriendly sensor for serotonin. 用聚马尿酸/多壁碳纳米管装饰的回收电池石墨作为血清素环保传感器的应用。

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-11-26 DOI: 10.1038/s41598-024-80673-y

Aya G Abd El-Nasser, Mahmoud G Metwally, Azza A Shoukry, Rasha M El Nashar

{"title":"Application of recycled battery graphite decorated with poly hippuric acid/ multiwalled carbon nanotubes as an ecofriendly sensor for serotonin.","authors":"Aya G Abd El-Nasser, Mahmoud G Metwally, Azza A Shoukry, Rasha M El Nashar","doi":"10.1038/s41598-024-80673-y","DOIUrl":"https://doi.org/10.1038/s41598-024-80673-y","url":null,"abstract":"A novel modified sensor based on electropolymerization of hippuric acid (HA) using cyclic voltammetry within the potential window - 1 to 1.5 V for 10 cycles at a scan rate 100 mV s-1 over multiwalled carbon nanotubes (MWCNTs) on battery graphite electrode (BGE). Poly (HA)/MWCNTs/BGE sensor exhibited two linearity ranges 3.00 × 10-3 to 1.00 µM (5.29 × 10-4 - 0.18 µg/ml) and 5.00 to 1.00 × 103 µM (0.88- 176.22 µg/ml) with limit of detection (LOD) of 0.06 × 10-2 µM (1.06 × 10-4 µg/ml) and limit of quantification (LOQ) of 2.00 × 10-3 µM (3.52 × 10-4 µg/ml). The poly (HA)/MWCNTs/BGE sensor was successfully applied to the determination of SER in the presence of tryptophan and in human blood serum with recovery ranges 98.31-105.47% with RSD values 3.02- 4.77%. Green chemistry metrics : national environmental index (NEMI), analytical greenness metric (AGREE), Raynie and Driver, green analytical procedure index (GAPI), and the analytical eco-scale were employed and indicated that the proposed sensor can be classified as an excellent green method, achieving an analytical eco-scale score of 84.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"29304"},"PeriodicalIF":3.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon. 脂质体和转录组的比较为了解经零价铁纳米粒子处理的双子叶弗氏菌提供了新的视角。

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-11-26 DOI: 10.1038/s41598-024-79780-7

Yavuz S Yalcin, Samson Gichuki, Huan Chen, Anithachristy Sigamani Arumanayagam, Shyama Malika Malwalage, Viji Sitther

{"title":"Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon.","authors":"Yavuz S Yalcin, Samson Gichuki, Huan Chen, Anithachristy Sigamani Arumanayagam, Shyama Malika Malwalage, Viji Sitther","doi":"10.1038/s41598-024-79780-7","DOIUrl":"https://doi.org/10.1038/s41598-024-79780-7","url":null,"abstract":"Understanding the intricate interplay between nanoparticle-mediated cyanobacterial interactions is pivotal in elucidating their impact on the transcriptome and lipidome. In the present study, total fatty acid methyl esters (FAMEs) in the wild-type (B481-WT) and transformant (B481-SD) Fremyella diplosiphon strains treated with nanoscale zero-valent iron nanoparticles (nZVIs) were characterized, and transcriptome changes analyzed. Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry revealed a 20-25% higher percentage of FAMEs in nZVI-treated F. diplosiphon strain B481-SD compared to B481-WT. Accumulation of alkanes was significantly higher (> 1.4 times) in both strains treated with 25.6 mg L-1 nZVIs compared to the untreated control. In addition, we observed significantly higher levels of monounsaturated FAMEs (11%) in B481-WT in 3.2 (11.34%) and 25.6 mg L-1 (11.22%) nZVI-treated cells when compared to the untreated control (7%). Analysis of the F. diplosiphon transcriptome treated with 3.2 mg L-1 revealed a total of 1811 and 1651 genes that were differentially expressed in B481-SD and B481-WT respectively. While the expression of iron uptake and ion channel genes was downregulated, genes coding for photosynthesis, pigment, and antioxidant enzymes were significantly (p < 0.05) upregulated in B481-SD treated with 3.2 mg L-1 nZVIs compared to the untreated control. This study on essential FAMEs and regulation of genes in nZVI-treated F. diplosiphon strains provides a molecular framework for optimization of metabolic pathways in this model species.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"29380"},"PeriodicalIF":3.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emoji multimodal microblog sentiment analysis based on mutual attention mechanism. 基于相互关注机制的表情符号多模态微博情感分析

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-11-26 DOI: 10.1038/s41598-024-80167-x

Yinxia Lou, Junxiang Zhou, Jun Zhou, Donghong Ji, Qing Zhang

引用次数: 0