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An open source, PCR based, point-of-care testing platform.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2025-04-08 DOI: 10.1038/s41598-025-95639-x

Yaping Xie, Zhou Sha, Shuaiwu Huang, Chiwei Yin, Liangcheng Wan, Jianjun Li, Jiangang Ling, Chang Wu, Lizhong Dai

{"title":"An open source, PCR based, point-of-care testing platform.","authors":"Yaping Xie, Zhou Sha, Shuaiwu Huang, Chiwei Yin, Liangcheng Wan, Jianjun Li, Jiangang Ling, Chang Wu, Lizhong Dai","doi":"10.1038/s41598-025-95639-x","DOIUrl":"https://doi.org/10.1038/s41598-025-95639-x","url":null,"abstract":"In recent years, point-of-care nucleic acid testing had played an important role in the diagnosis of infectious diseases. Despite its advantages, current point-of-care testing (POCT) platforms were all closed, which hindered researchers from quickly validating the performance of customized reagents. Incompatibility and repeated development of different POCT devices also resulted in the waste of medical resources. In this article, a rotating cartridge with a detachable reagent rack was developed, which provided an open platform in conjunction with a programmable POCT instrument. The system had been confirmed to effectively detect the HPV sample with a concentration of 1000 copies per milliliter and the novel coronavirus nucleic acid control dilution with a concentration of 200 copies per milliliter within 1 h. The cartridge could detect six targets simultaneously and cost less than ten dollars. The square of the correlation coefficients of the fitting line between the dye concentration and the fluorescence intensity all exceeded 0.99. The coefficient of variation (CV) for testing one sample with multiple cartridges or instruments was under 3%, confirming the stability and reliability of system performance. Results of clinical testing further verified its ability to detect multiple viruses, including novel coronavirus, influenza and human papillomavirus. Compared with standard results, the sensitivity reached 100% in the detection of multiple respiratory viruses and HPV viruses while the specificity was above 98% for all targets. The area under the curve (AUC) values for all receiver operating characteristic (ROC) curves exceeded 0.98. These results indicated that this platform had excellent performances and provided a competitive solution for low-cost, open point-of-care nucleic acid testing.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"12025"},"PeriodicalIF":3.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Menstrual blood serum extracellular vesicles reveal novel molecular biomarkers and potential endotypes of unexplained infertility.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2025-04-08 DOI: 10.1038/s41598-025-95818-w

Kieran Brennan, Raminta Vaiciuleviciute, Ilona Uzieliene, Jolita Pachaleva, Zaneta Kasilovskiene, Lina Piesiniene, Eiva Bernotiene, Margaret M Mc Gee

{"title":"Menstrual blood serum extracellular vesicles reveal novel molecular biomarkers and potential endotypes of unexplained infertility.","authors":"Kieran Brennan, Raminta Vaiciuleviciute, Ilona Uzieliene, Jolita Pachaleva, Zaneta Kasilovskiene, Lina Piesiniene, Eiva Bernotiene, Margaret M Mc Gee","doi":"10.1038/s41598-025-95818-w","DOIUrl":"https://doi.org/10.1038/s41598-025-95818-w","url":null,"abstract":"Several biomolecules have been previously associated with unexplained infertility (uIF) in blood and uterine samples, immune cells and their secreted factors, endometrial tissue, menstrual blood, serum, and stromal cells, however they do not comprehensively represent different uIF endotypes and their isolation/detection involves invasive diagnostic methods and lacks precision. This ex-vivo study was performed on extracellular vesicles (EVs) from menstrual blood collected on cycle day 2 from 9 fertile volunteers and 8 women with uIF. Menstrual blood serum (MBS) EVs were isolated from fertile and uIF MBS using Iodixanol Density Gradient Centrifugation and quantified by flow cytometry. EVs were characterized according to MISEV2023 guidelines. Comprehensive proteomic analysis of MBS EVs and EV-depleted MBS showed significant changes in uIF proteome, mostly affecting cell adhesion, immune response, apoptosis, response to oxidative stress and lipid metabolism. These processes were previously linked to pathologies of the female reproductive system but never investigated in uIF and were used to stratify patients into distinct molecular endotypes. Area under the curve (AUC) analysis was used to determine the optimum set of biomarkers for each of the uIF molecular endotypes. These findings provide new insights into uIF that could facilitate personalised treatment approaches.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11974"},"PeriodicalIF":3.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: Comprehensive analysis of mitochondrial-related gene signature for prognosis, tumor immune microenvironment evaluation, and candidate drug development in colon cancer.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2025-04-08 DOI: 10.1038/s41598-025-97128-7

Hao Wu, Wentao Zhang, Jingjia Chang, Jin Wu, Xintong Zhang, Fengfeng Jia, Li Li, Ming Liu, Jianjun Zhu

引用次数: 0

Comparative diabetes mellitus burden trends across global, Chinese, US, and Indian populations using GBD 2021 database.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2025-04-08 DOI: 10.1038/s41598-025-96175-4

Yafei Chen, Guoyu Wang, Zhiyong Hou, Xinxin Liu, Siyi Ma, Min Jiang

{"title":"Comparative diabetes mellitus burden trends across global, Chinese, US, and Indian populations using GBD 2021 database.","authors":"Yafei Chen, Guoyu Wang, Zhiyong Hou, Xinxin Liu, Siyi Ma, Min Jiang","doi":"10.1038/s41598-025-96175-4","DOIUrl":"https://doi.org/10.1038/s41598-025-96175-4","url":null,"abstract":"Diabetic mellitus (DM) poses a significant challenge and stress to global health, comparing the burden of disease in the world's three most populous countries while projecting changes in trends in age-standardized rate (ASR) -deaths and disability adjusted life years (DALYs) up to 2050. Using GBD2021 data, we examined DM trends in China, US, India and globally for 1990-2021, and projected deaths and DALYs for DM (types 1 and 2) for 2022-2050 using Bayesian age-period-cohort (BAPC) model. It was found that the ASR-DALYs and deaths for T1DM are trending downward globally, while those for T2DM are trending upward. In terms of gender differences, the burden of T1DM by gender was insignificant, whereas the burden of disease was significantly higher in men with T2DM than in women. The burden of disease for T1DM peaks around the ages of 40-44 years, while the burden of disease for T2DM peaks at 65-69 years. Population growth and ageing are major factors influencing the disease burden of diabetes. The projection of ASR-deaths and DALYs globally for 2022-2050 showed a decreasing trend in T1DM and an increasing trend in T2DM (especially in China and India). The increasing burden of T2DM disease globally and in three countries by 2050 should be taken seriously.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11955"},"PeriodicalIF":3.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental overproduction of cortical superficial neurons impairs adult auditory cortical processing.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2025-04-08 DOI: 10.1038/s41598-025-95968-x

Mirna Merkler, Nancy Y Ip, Shuzo Sakata

{"title":"Developmental overproduction of cortical superficial neurons impairs adult auditory cortical processing.","authors":"Mirna Merkler, Nancy Y Ip, Shuzo Sakata","doi":"10.1038/s41598-025-95968-x","DOIUrl":"https://doi.org/10.1038/s41598-025-95968-x","url":null,"abstract":"While evolutionary cortical expansion is thought to underlie the evolution of human cognitive capabilities, excessive developmental expansion can lead to megalencephaly, often found in neurodevelopmental disorders. Still, little is known about how the overproduction of cortical neurons during development affects cortical processing and behavior in later life. Here we show that developmental overproduction of cortical superficial neurons impairs auditory processing in adult mice. We applied XAV939 to overproduce cortical superficial excitatory neurons during development. XAV939-treated adult mice exhibited auditory behavioral deficits and abnormal auditory cortical processing. Furthermore, we found fewer functional monosynaptic connections between cortical putative excitatory neurons. Altogether, our results suggest that abnormal auditory cortical processing contributes to the atypical auditory detectability in XAV939-treated mice. Although the expansion of cortical size is evolutionarily advantageous, an abnormal expansion during development can result in detrimental effects on cortical processing and perceptual behavior in adulthood.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11993"},"PeriodicalIF":3.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of environment on adolescents' physical exercise behavior based on family community and school micro-systems.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2025-04-08 DOI: 10.1038/s41598-025-91387-0

Zhaojin Li, Shanshan Xie, Weifu Chen

引用次数: 0

Novel serum biomarker associations with 7 Tesla MRI-defined cortical lesions, leptomeningeal enhancement, and deep gray matter volume in early multiple sclerosis.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2025-04-08 DOI: 10.1038/s41598-025-95229-x

Andrei Miclea, Jonathan Zurawski, Brian C Healy, Shrishti Saxena, Hrishikesh Lokhande, Molly Quattrucci, Renxin Chu, Howard L Weiner, Rohit Bakshi, Tanuja Chitnis

{"title":"Novel serum biomarker associations with 7 Tesla MRI-defined cortical lesions, leptomeningeal enhancement, and deep gray matter volume in early multiple sclerosis.","authors":"Andrei Miclea, Jonathan Zurawski, Brian C Healy, Shrishti Saxena, Hrishikesh Lokhande, Molly Quattrucci, Renxin Chu, Howard L Weiner, Rohit Bakshi, Tanuja Chitnis","doi":"10.1038/s41598-025-95229-x","DOIUrl":"https://doi.org/10.1038/s41598-025-95229-x","url":null,"abstract":"Gray matter demyelinating lesions, brain atrophy and meningeal inflammation are hypothesized to be relevant in multiple sclerosis (MS) disease pathogenesis, though their relationship to immune alterations in early MS is not well characterized. This study aims to investigate correlations between the concentrations of 112 serum proteins and 7 Tesla MRI-defined measures of disease severity in patients with early MS. In this analysis, patients with CIS or MS having a 7 Tesla brain MRI and blood sample both within five years of MS diagnosis were included (n = 57). Correlational analysis was adjusted for sex, age, and disease duration. Correlation between serum proteins and MRI-defined cortical and thalamic gray matter lesions, leptomeningeal enhancement (presence and foci number), deep gray matter (DGM) structure volumes, whole brain parenchymal volume and total T2 white matter lesion volume was assessed. In this study, cortical lesions were associated with higher IL-15, TNF-alpha, and BAFF levels, and lower levels of FcRL2. Leptomeningeal enhancement was associated with higher levels of PLXNB3 and lower levels of nCDase and CNTN5. Higher IL-1B levels correlated with lower DGM volume while higher levels of CDH6, SIGLEC9, and HAGH correlated with higher DGM volume. These novel associations between serum immune proteins and 7 T MRI outcomes may have relevance as disease biomarkers in early stages of MS.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"12032"},"PeriodicalIF":3.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Numerical study on falling film flow outside the refrigerant tube.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2025-04-08 DOI: 10.1038/s41598-025-96057-9

Longfei Dong, Rui Zhang

{"title":"Numerical study on falling film flow outside the refrigerant tube.","authors":"Longfei Dong, Rui Zhang","doi":"10.1038/s41598-025-96057-9","DOIUrl":"https://doi.org/10.1038/s41598-025-96057-9","url":null,"abstract":"The liquefied natural gas (LNG) floating production storage and offloading (FPSO) unit is a new type of floating production device developed for the exploitation, pretreatment, liquefaction, and storage of offshore natural gas. In this study, the shell side structure of the spiral-wound heat exchanger is analyzed, and the effects of varying the Reynolds number (Re), tube outer diameter, and number of distributors on the thickness of the shell-side liquid film are investigated. The results show that as the fluid flows down from the distributor, it hits the upper wall of the pipeline and diffuses evenly to both sides, before converging in between the two distributors. In the axial direction, the thickness of the liquid film increases first, reaches the highest at the peak, then decreases, reaches the lowest at the trough, and then increases again, forming a secondary peak at the drop. The thickness of the liquid film changes periodically, and the period is the distance between the two distributors. The thickness of the circumferential liquid film is negatively correlated with the circumferential angle, α. Moreover, the liquid film is thinnest at α = 120°, and is positively correlated with both the liquid mass flow rate and the outer diameter of the tube. The most uniform liquid film thickness is obtained when Re = 1500, the tube outer diameter is 12 mm, and the number of distributors is 6. The results from this study can guide the design of spiral-wound heat exchangers and facilitate their safe and efficient operation in natural gas liquefaction processes.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11952"},"PeriodicalIF":3.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On a Bayesian multivariate survival tree approach based on three frailty models.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2025-04-08 DOI: 10.1038/s41598-025-96198-x

Patcharaporn Porndumnernsawat, Till D Frank, Lily Ingsrisawang

{"title":"On a Bayesian multivariate survival tree approach based on three frailty models.","authors":"Patcharaporn Porndumnernsawat, Till D Frank, Lily Ingsrisawang","doi":"10.1038/s41598-025-96198-x","DOIUrl":"https://doi.org/10.1038/s41598-025-96198-x","url":null,"abstract":"This study compared the performance of the Bayesian multivariate survival tree approach constructed from extended Cox proportional hazard with gamma frailty term, and two shared gamma frailty models with exponential and Weibull baseline hazard function, respectively. A simulation study was applied to evaluate the impact of the baseline hazard function, number of clusters (200, 500, 1000), cluster size (5, 10, 20), and right censoring rate (10%, 50%, 80%) on the performance of classification. We generated 90 clustered survival datasets having correlated failure times and 50 covariates at cluster level and at individual level. Each dataset was resampling 1000 times by selecting clusters at random 70% as training datasets and the rest 30% as the test datasets. The performance of a Bayesian multivariate survival tree approach based on shared gamma frailty models with Weibull distribution provided the highest accuracy. All three models, the accuracy tended to increase with an increase in the cluster size and the number of clusters. The accuracy decreased monotonically with increasing the percentage of censoring rate. In conclusion, the use of the Bayesian multivariate survival tree approach constructed from the shared gamma frailty with baseline hazard function as Weibull distribution was recommended.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"12017"},"PeriodicalIF":3.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving an endangered marine species distribution using reliable and localized environmental DNA detections combined with trawl captures.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2025-04-08 DOI: 10.1038/s41598-025-95358-3

Marion Chevrinais, Audrey Bourret, Geneviève Côté, Geneviève Faille, Nellie Gagné, Geneviève J Parent

{"title":"Improving an endangered marine species distribution using reliable and localized environmental DNA detections combined with trawl captures.","authors":"Marion Chevrinais, Audrey Bourret, Geneviève Côté, Geneviève Faille, Nellie Gagné, Geneviève J Parent","doi":"10.1038/s41598-025-95358-3","DOIUrl":"10.1038/s41598-025-95358-3","url":null,"abstract":"The description of marine fish distributions generally relies on trawl survey observations. For rare species, sporadic catches necessitate the combination of multiannual trawl surveys to accurately describe the distribution, limiting short term monitoring. Recent studies suggest that combining traditional capture methods and environmental DNA (eDNA) detections enhance rare species' occurrence. In this study, the reliability and localization of eDNA detections (single- and multi-species) of an endangered marine species, the Atlantic wolffish Anarhichas lupus, was assessed during fine scale surveys. eDNA was detected at all six stations sampled with Niskin bottles over caves housing one or two A. lupus. Detections from samples collected with syringes by divers along a 15 m transect perpendicular to each cave were limited to the fish cave entrance. Trawl-captures and eDNA detections were then combined to test if the species distribution is improved for broad scale surveys. The station-based frequency of species occurrence was 13% with trawl captures and increased to 23% when combined with eDNA detections. Single-species detections were generally more sensitive than multi-species detections. Our results showed that a rare marine species distribution improves combining traditional methods and eDNA detections in oceanographic surveys. Strategies for integrating optimal eDNA detections in marine surveys are discussed.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11926"},"PeriodicalIF":3.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0