Scientific Reports最新文献_第9页

Spinal instrumentation length affects adjacent segment range of motion and intradiscal pressure.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-12-16 DOI: 10.1038/s41598-024-82132-0

Christian Liebsch, Peter Obid, Morten Vogt, Benedikt Schlager, Hans-Joachim Wilke

{"title":"Spinal instrumentation length affects adjacent segment range of motion and intradiscal pressure.","authors":"Christian Liebsch, Peter Obid, Morten Vogt, Benedikt Schlager, Hans-Joachim Wilke","doi":"10.1038/s41598-024-82132-0","DOIUrl":"https://doi.org/10.1038/s41598-024-82132-0","url":null,"abstract":"Scoliosis instrumentation length depends on the type and degree of deformity and the individual preference of the surgeon. This in vitro study aimed to explore effects of increasing instrumentation length on adjacent segment mobility and intervertebral disc loading. Six fresh frozen human spine specimens (C7-sacrum) with entire rib cage from young adult donors (26-45 years) were loaded with pure moments of 5 Nm. Range of motion (ROM) of all segments was determined using optical motion tracking. Lumbar intradiscal pressure (IDP) was measured using flexible pressure sensors from L1 to L5. The specimens were tested in two groups with increasing posterior instrumentation length in proximal (group 1) and distal direction (group 2). Significant (p < 0.05) adjacent segment ROM increases compared to the condition without any instrumentation and compared to other instrumentations were primarily found proximally to the instrumentation in lateral bending. IDP significantly (p < 0.05) increased in flexion in the distal adjacent segment for T4-L1 instrumentation and by up to 550% at instrumented levels compared to the condition without instrumentation. These findings may explain clinical complications such as adjacent segment disease and associated proximal and distal junctional kyphosis. To reduce loads on adjacent segments, instrumentation should therefore be applied as short as reasonable.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30496"},"PeriodicalIF":3.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Renal phenotyping in a hypomorphic murine model of propionic aciduria reveals common pathomechanisms in organic acidurias.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-12-16 DOI: 10.1038/s41598-024-79572-z

Anke Schumann, Ainhoa Martinez-Pizarro, Eva Richard, Christoph Schell, Anna Laura Kössinger, Karina A Zeyer, Stefan Tholen, Oliver Schilling, Michael Barry, Björn Neubauer, Michael Köttgen, Luciana Hannibal, Lourdes R Desviat, Ute Spiekerkötter

{"title":"Renal phenotyping in a hypomorphic murine model of propionic aciduria reveals common pathomechanisms in organic acidurias.","authors":"Anke Schumann, Ainhoa Martinez-Pizarro, Eva Richard, Christoph Schell, Anna Laura Kössinger, Karina A Zeyer, Stefan Tholen, Oliver Schilling, Michael Barry, Björn Neubauer, Michael Köttgen, Luciana Hannibal, Lourdes R Desviat, Ute Spiekerkötter","doi":"10.1038/s41598-024-79572-z","DOIUrl":"https://doi.org/10.1038/s41598-024-79572-z","url":null,"abstract":"Mutations in the mitochondrial enzyme propionyl-CoA carboxylase (PCC) cause propionic aciduria (PA). Chronic kidney disease (CKD) is a known long-term complication. However, good metabolic control and standard therapy fail to prevent CKD. The pathophysiological mechanisms of CKD are unclear. We investigated the renal phenotype of a hypomorphic murine PA model (Pcca-/-(A138T)) to identify CKD-driving mechanisms. Pcca-/-(A138T) mice show elevated retention parameters and express markers of kidney damage progressing with time. Morphological assessment of the Pcca-/-(A138T) mouse kidneys indicated partial flattening of tubular epithelial cells and focal tubular-cystic dilation. We observed altered renal mitochondrial ultrastructure and mechanisms acting against oxidative stress were active. LC-MS/MS analysis confirmed disease-specific metabolic signatures and revealed disturbances in mitochondrial energy generation via the TCA cycle. Our investigations revealed altered mitochondrial networks shifted towards fission and a marked reduction of mitophagy. We observed a steep reduction of PGC-1-α, the key mediator modulating mitochondrial functions and a counter actor of mitochondrial fission. Our results suggest that impairment of mitochondrial homeostasis and quality control are involved in CKD development in PA. Therapeutic targeting of the identified pathways might help to ameliorate CKD in addition to the current treatment strategies.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30478"},"PeriodicalIF":3.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: GLCCI1 alleviates GRP78-initiated endoplasmic reticulum stress-induced apoptosis of retinal ganglion cells in diabetic retinopathy by upregulating and interacting with HSP90AB1.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-12-16 DOI: 10.1038/s41598-024-83001-6

Jiayou Liu, Hongdan Yu, Shengxue Yu, Mengren Liu, Xinyuan Chen, Yufei Wang, Jing Li, Caixing Shi, Wenqiang Liu, Zhongfu Zuo, Xuezheng Liu

引用次数: 0

Author Correction: Novel synthesis of CuHCF/B-rGO composites for oxygen evolution reaction activity.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-12-16 DOI: 10.1038/s41598-024-82947-x

S Swathi, R Yuvakkumar, L Kungumadevi, G Ravi, Dhayalan Velauthapillai

引用次数: 0

Run-off impacts on Arctic kelp holobionts have strong implications on ecosystem functioning and bioeconomy. 径流对北极海带全生物的影响对生态系统功能和生物经济具有重大影响。

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-12-16 DOI: 10.1038/s41598-024-82287-w

Sarina Niedzwiedz, Claudia Schmidt, Yunlan Yang, Bertille Burgunter-Delamare, Sebastian Andersen, Lars Hildebrandt, Daniel Pröfrock, Helmuth Thomas, Rui Zhang, Børge Damsgård, Kai Bischof

{"title":"Run-off impacts on Arctic kelp holobionts have strong implications on ecosystem functioning and bioeconomy.","authors":"Sarina Niedzwiedz, Claudia Schmidt, Yunlan Yang, Bertille Burgunter-Delamare, Sebastian Andersen, Lars Hildebrandt, Daniel Pröfrock, Helmuth Thomas, Rui Zhang, Børge Damsgård, Kai Bischof","doi":"10.1038/s41598-024-82287-w","DOIUrl":"https://doi.org/10.1038/s41598-024-82287-w","url":null,"abstract":"Kelps (Laminariales, Phaeophyceae) are foundation species along Arctic rocky shores, providing the basis for complex ecosystems and supporting a high secondary production. Due to ongoing climate change glacial and terrestrial run-off are currently accelerating, drastically changing physical and chemical water column parameters, e.g., water transparency for photosynthetically active radiation or dissolved concentrations of (harmful) elements. We investigated the performance and functioning of Arctic kelp holobionts in response to run-off gradients, with a focus on the effect of altered element concentrations in the water column. We found that the kelp Saccharina latissima accumulates harmful elements (e.g., cadmium, mercury) originating from coastal run-off. As kelps are at the basis of the food web, this might lead to biomagnification, with potential consequences for high-latitude kelp maricultures. In contrast, the high biosorption potential of kelps might be advantageous in monitoring environmental pollution or potentially extracting dissolved rare earth elements. Further, we found that the relative abundances of several kelp-associated microbial taxa significantly responded to increasing run-off influence, changing the kelps functioning in the ecosystem, e.g., the holobionts nutritional value and elemental cycling. The responses of kelp holobionts to environmental changes imply cascading ecological and economic consequences for Arctic kelp ecosystems in future climate change scenarios.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30506"},"PeriodicalIF":3.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Late Holocene cooling drove drastic decreases in cladoceran diversity in a subarctic lake.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-12-16 DOI: 10.1038/s41598-024-81690-7

María de Los Ángeles González Sagrario, Tobias Vrede, Simon Belle

{"title":"Late Holocene cooling drove drastic decreases in cladoceran diversity in a subarctic lake.","authors":"María de Los Ángeles González Sagrario, Tobias Vrede, Simon Belle","doi":"10.1038/s41598-024-81690-7","DOIUrl":"https://doi.org/10.1038/s41598-024-81690-7","url":null,"abstract":"Subarctic lakes are sentinels of climate change, showing responses in their physical, chemical, and biological properties. However, climate-induced changes in invertebrate diversity and their underlying mechanisms are not fully understood. We explored the relationship between past climate change and taxonomic composition of subfossil cladocerans in a subarctic lake during the last ca. 5700 years. The Cladocera community shifted from specialist to generalist species at ca. 3500 cal years BP, corresponding to the long-term cooling period between the Holocene Thermal Maximum and the Late Holocene. Taxonomic diversity declined driven by the collapse of the keystone herbivorous Daphnia longispina group, pelagic and littoral predators, and phytophilous benthic species, therefore resulting in a simplification of the food web and a reduction of trophic levels. Furthermore, the shift in cladoceran composition was associated with the decline of aquatic primary producers and the development of birch forest, suggesting a potential causal link between dissolved organic carbon dynamics and cladoceran community composition. This study provides empirical evidence of the response of cladocerans to climatic fluctuations and their underlying mechanisms through catchment-mediated processes and direct temperature-induced changes.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30490"},"PeriodicalIF":3.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of interferon type I on barrier function of human airway epithelium during rhinovirus infections in vitro.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-12-16 DOI: 10.1038/s41598-024-82516-2

Helena Boland, Adrian Endres, Ralf Kinscherf, Ralf Schubert, Beate Wilhelm, Hans Schwarzbach, Danny Jonigk, Peter Braubach, Gernot Rohde, Carla Bellinghausen

{"title":"Protective effect of interferon type I on barrier function of human airway epithelium during rhinovirus infections in vitro.","authors":"Helena Boland, Adrian Endres, Ralf Kinscherf, Ralf Schubert, Beate Wilhelm, Hans Schwarzbach, Danny Jonigk, Peter Braubach, Gernot Rohde, Carla Bellinghausen","doi":"10.1038/s41598-024-82516-2","DOIUrl":"https://doi.org/10.1038/s41598-024-82516-2","url":null,"abstract":"The airway epithelium provides a crucial barrier against infection with respiratory pathogens. This barrier can be impaired following viral infection, paving the way for bacterial superinfections. Type I interferons (IFNs) are important antiviral mediators, and inhaled formulations of these glycoproteins are considered a potential approach for the treatment of respiratory viral infections. To investigate if type I IFNs can also protect against virus-induced epithelial barrier dysfunction, differentiated primary bronchial epithelial cells were pre-treated with IFN-β1a and subsequently infected with human rhinovirus (HRV) for 24 to 72h. Moreover, to functionally assess the effects of IFN-β1a pre-treatment on barrier integrity, we conducted co-infection experiments, in which cells were initially infected with HRV, and superinfected with Streptococcus pneumoniae 24 to 72 h later. In untreated cells, HRV infection significantly damaged ZO-1 positive tight junctions and cilia, and transiently increased permeability, whereas the barrier of cultures pre-treated with IFN-β1a remained intact. In co-infection experiments, bacteria were able to penetrate deeper into the cell layers of HRV-infected cultures than into those of uninfected cells. IFN-β1a pre-treatment abrogated virus-induced damage to the epithelial barrier. Taken together, these data demonstrate a beneficial effect of IFN-β in protecting epithelial barrier function in addition to its antiviral effects.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30510"},"PeriodicalIF":3.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: Ultrasound based radiomics model for assessment of placental function in pregnancies with preeclampsia.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-12-16 DOI: 10.1038/s41598-024-81772-6

Hongshuang Sun, Jing Jiao, Yicong Wang, Chen Zhu, Shaochun Wang, Yuanyuan Wang, Bo Ban, Yi Guo, Yunyun Ren

引用次数: 0

Date seeds powder alleviate the aflatoxin B1 provoked heart toxicity in male offspring rat. 枣核粉能减轻黄曲霉毒素 B1 对雄性后代大鼠心脏的毒性。

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-12-16 DOI: 10.1038/s41598-024-80197-5

Heba M A Khalil, Walaa A M Eid, Mohammad El-Nablaway, Eman Mohamad El Nashar, Jaber Saad Al-Tarish, Hanan M A El Henafy

{"title":"Date seeds powder alleviate the aflatoxin B1 provoked heart toxicity in male offspring rat.","authors":"Heba M A Khalil, Walaa A M Eid, Mohammad El-Nablaway, Eman Mohamad El Nashar, Jaber Saad Al-Tarish, Hanan M A El Henafy","doi":"10.1038/s41598-024-80197-5","DOIUrl":"https://doi.org/10.1038/s41598-024-80197-5","url":null,"abstract":"Date (Phoenix dactylifera L.) seeds (PDL) have recently evoked significant attention for their therapeutic potential against numerous diseases. Aflatoxin B1 (AFB1) is an inevitable environmental hazard that pollutes foods and may harm the heart. This study investigated the beneficial effect of PDL against cardiac toxicity induced by AFB1 in male offspring. Female albino rats received PDL (200 mg/kg) orally for 14 days before mating till weaning and AFB1 (50 μg/kg) intramuscularly throughout gestation and lactation. At postnatal day 60, male offspring hearts were collected. Compared to AFB1 intoxicated group, PDL-treated offspring displayed improved cardiac biomarkers, an increase in their antioxidant defense, and a decrease in the cardiac proinflammatory cytokines. Additionally, a reduction in the expression levels of Bcl2 and Nrf2 was observed, with genes linked to increased cardiac caspase-3, Bax, ACE1, P53, and cytochrome C levels. In conclusion, PDL acts as a potential adjuvant agent for ameliorating cardiac toxicity and apoptosis resulting from exposure to AFB1. This is attributed to its antioxidative and anti-inflammatory effects, as well as its capacity to sequester free radicals within cardiac tissue.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30480"},"PeriodicalIF":3.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A quantitative multi-parameter mapping protocol standardized for clinical research in multiple sclerosis.

IF 3.8 2区综合性期刊

Scientific Reports Pub Date : 2024-12-16 DOI: 10.1038/s41598-024-80274-9

Henri Trang, Tim J Hartung, Qianlan Chen, Stefan Hetzer, Claudia Chien, Pia S Sperber, Tanja Schmitz-Hübsch, Susanna Asseyer, Rebekka Rust, Darius Mewes, Lina Anderhalten, Michael Sy, Alexander U Brandt, Carsten Finke, Friedemann Paul

{"title":"A quantitative multi-parameter mapping protocol standardized for clinical research in multiple sclerosis.","authors":"Henri Trang, Tim J Hartung, Qianlan Chen, Stefan Hetzer, Claudia Chien, Pia S Sperber, Tanja Schmitz-Hübsch, Susanna Asseyer, Rebekka Rust, Darius Mewes, Lina Anderhalten, Michael Sy, Alexander U Brandt, Carsten Finke, Friedemann Paul","doi":"10.1038/s41598-024-80274-9","DOIUrl":"https://doi.org/10.1038/s41598-024-80274-9","url":null,"abstract":"Quantitative magnetic resonance imaging (qMRI) involves mapping microstructure in standardized units sensitive to histological properties and supplements conventional MRI, which relies on contrast weighted images where intensities have no biophysical meaning. While measuring tissue properties such as myelin, iron or water content is desired in a disease context, qMRI changes may typically reflect mixed influences from aging or pre-clinical degeneration. We used a fast multi-parameter mapping (MPM) protocol for clinical routine at 3T to reconstruct whole-brain quantitative maps of magnetization transfer saturation (MT), proton density (PD), longitudinal (R1), and transverse relaxation rate (R2*) with 1.6 mm isotropic resolution. We report reference MPM values from a healthy population with age and gender distributions typical of multiple sclerosis in whole brain white matter (WM), T2-weighted WM hyperintensities, cortical grey matter and deep grey matter regions and present post-processing optimizations including integration of lesions and normalization of PD maps against cerebrospinal fluid (CSF) for standardized research in multiple sclerosis (MS) and potentially also in related disorders. PD maps were affected by WM abnormalities in MS using WM calibration. The results acknowledge the impact of non-linear age effects on MPM and suggest using CSF calibration for future clinical application in MS.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30481"},"PeriodicalIF":3.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0