Scientific Reports最新文献

{"title":"Prevalence and drug resistance profiles of nontuberculous mycobacteria isolated from Iranian fish pond waters.","authors":"Xiaqian Cui, Yu Zhang, Yancui Cai, Sale Zhang, Ying Li, Haifang Wang, Fatemeh Sakhaee, Seyed Davar Siadat, Abolfazl Fateh","doi":"10.1038/s41598-025-20286-1","DOIUrl":"https://doi.org/10.1038/s41598-025-20286-1","url":null,"abstract":"","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36363"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing the normal and epileptic output of a neural mass model based on cyclic-small gain theorem.","authors":"Sina Hosseini, Abolfazl Yaghmaei, Fariba Bahrami, Mohammad Javad Yazdanpanah","doi":"10.1038/s41598-025-20315-z","DOIUrl":"https://doi.org/10.1038/s41598-025-20315-z","url":null,"abstract":"<p><p>This study presents a new application of the Cyclic-Small Gain Theorem (CSGT) to analyze the behavior of a Neural Mass Model (NMM), which is utilized to study brain activity related to epilepsy. The model is reformulated as an interconnected network of dynamic subsystems, which made CSGT applicable to this context. It is shown that whenever the CSGT conditions are satisfied, the model is input-to-state stable, and epilepsy cannot occur. Furthermore, the proposed method guarantees normal activity even with increased amplitude of noise, as long as the stability conditions of CSGT are verified. In this way, system instability can be considered indicative of the occurrence of epilepsy. The behavior of an interconnected network consisting of two epileptic columns and a healthy column is analyzed using the proposed method to study the propagation of epileptic activity between regions. The results show that, when the healthy column satisfies the CSGT conditions, it remains stable and unaffected by its epileptic counterparts. The study emphasizes the importance of the CSGT as a protective measure against epileptic activity and provides insights for designing control interventions using electric brain stimulation.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36412"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A mutation detection method of discontinuous structures in rock strata based on drilling parameters.","authors":"Xin Sun, Jingyi Cheng, Zhijun Wan, Zhiqing Guo, Shuruo Zhang, Shuheng Wang","doi":"10.1038/s41598-025-20423-w","DOIUrl":"https://doi.org/10.1038/s41598-025-20423-w","url":null,"abstract":"<p><p>Discontinuous structures in coal mine roadway roofs, such as rock interfaces and joint fractures, are critical factors leading to surrounding rock instability. The use of Measurement While Drilling (MWD) technology to identify geological formations has become a growing trend. However, there is still a lack of rock structure recognition methods that offer high accuracy, efficiency, and strong generalizability. Therefore, this study acquired four drilling parameters including thrust, torque, modulation specific energy (SEM), and rock drillability assessment (RDA) through drilling experiments. By leveraging the Bayes algorithm, which has high precision, efficiency, and low cost, a change point detection model for drilling parameters was established, and a multi-parameter fusion criterion was proposed for identifying rock structures. The results show that for single rock interface identification, the errors of thrust, torque, SEM, and RDA were 13.3 mm, 4.6 mm, 4.4 mm, and 18.3 mm, respectively. For multiple rock interface identification, the recognition rates were 83.3%, 100.0%, 66.7%, and 83.3%, respectively. Moreover, the absolute value of the magnitude index (SLP) at the interface location was generally the highest among all change points. In multi-change-point detection, the SLP threshold should be set at ± 0.2. It is worth noting that the SLP value is correlated with data fluctuation intensity; greater fluctuation leads to higher SLP values at change points. This study contributes significantly to enabling intelligent perception of rock structures and improving the quality of rock mass control.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36447"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A clinical scoring system to predict tuberculous lymphadenitis in settings with high disease burden.","authors":"Ermias Teklehaimanot Yefter, Asefa Adimasu Taddese, Getahun Mengistu Tessema, Miteku Andualem Limenih, Alemante Tafese Beyna, Addisu Minaye Dejen, Destaye Shiferaw Alemu","doi":"10.1038/s41598-025-20322-0","DOIUrl":"https://doi.org/10.1038/s41598-025-20322-0","url":null,"abstract":"<p><p>Limited diagnostic service availability challenges the timely diagnosis and treatment of tuberculous lymphadenitis, the most common extrapulmonary tuberculosis in endemic areas. To address this, our study aimed to develop a clinical scoring rule for estimating the likelihood of tuberculous lymphadenitis, designed as a simple and easy-to-apply classification tool to facilitate timely diagnosis. We used a cross-sectional study to collect data from 1364 patients with lymphadenopathy. A prediction model was developed by incorporating predictors from multivariable logistic regression analysis. Optimal cutoff point was determined using Youden's Index. Performance was evaluated using discrimination and calibration. Internal validation and clinical utility were assessed using bootstrapping and decision curve analysis, respectively. A total of 790 patients (57.9%, 95% CI 55.3%-60.5%) were diagnosed with tuberculous lymphadenitis. Sex, duration, size, location, and consistency of lymphadenopathy, presence of pain, sinus tract, systemic symptoms, and erythrocyte sedimentation rate were predictors incorporated to develop the scoring rule. With a discrimination power of 96.9% (95% CI 96.1%, 97.8%), the clinical scoring rule using these predictors showed a sensitivity, specificity, and accuracy of 93.7%, 89.7%, and 92%, respectively. It demonstrated internal validity with near-zero optimism coefficients for various performance metrics. Using easily accessible clinical parameters, we developed a scoring rule that reliably predicts tuberculous lymphadenitis. Besides its use in clinical settings for making timely clinical decisions, the scoring rule is a valuable candidate for community-level screening, facilitating early case detection.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36439"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences and fingerprints of ESBL-producing E. coli from chicken faeces.","authors":"Nisa Sipahi, Yasemin Numanoglu Cevik","doi":"10.1038/s41598-025-14114-9","DOIUrl":"https://doi.org/10.1038/s41598-025-14114-9","url":null,"abstract":"<p><p>E. coli is an important source of β-lactam resistance. Extended Spectrum β-Lactamase (ESBL) producing E. coli and their phylogenetic transmission are an important problem for health. On the other hand, MALDI-TOF MS allows sensitive and specific applications in food safety and many clinical studies, and it is also a method approved by the Food and Drug Administration (FDA) for microbial identification. We thought that the protein phenotypic character of the bacteria might provide data on resistance. This study aims to investigate β-lactamases in E. coli and to examine the differences in protein properties of ESBL-producing bacteria. In the present study that is the first report for Duzce (Türkiye), 28.6% of 122 isolates were identified as Chicken Faeces E. coli (CFEC)-ESBL. bla_CTX-M, bla_CTX-M-1, bla_CTX-M-15, bla_SHV, bla_TEM, bla_OXA-10, bla_CIT and bla_MOX genes were determined with PCR and bla_CTX-M gene was detected with the highest rate (88.5%). At least one of the resistance genes was detected in the phenotype screening tests, except one of the isolates (CFEC-ESBL-90). On the other hand, CFEC-ESBL-38 contained bla_CTX-M-15 and the fact that this isolate was the only atypical ESBL strain with indole (-) and lac (-) characteristics among all isolates explains the highest variance (41%). It was also different from other Principle Component Analysis (PCA) components. Moreover, this isolate had a high degree of similarity (87%; CCI) with the other isolate (CFEC-ESBL-90), which had low similarity to CFEC-ESBLs. The study determined the differences and similarities between E. coli isolates with all PCA analyses. Our findings indicate that the ESBL group generally differed from susceptible strains, and the isolates had some heterogeneities and homogeneities. As a result, phyloproteomic analyses with MALDI-TOF MS are considered beneficial in characterising phenotypic bacterial behaviour. Despite that, there were some limitations in the study that need to be solved with further research.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36310"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental investigation of al₂o₃ nanoparticle-enhanced natural ester oil as a biodegradable liquid dielectric for transformers.","authors":"S Marisargunam, T Mariprasath, P Saraswathi, Mohit Bajaj, Milkias Berhanu Tuka","doi":"10.1038/s41598-025-16210-2","DOIUrl":"https://doi.org/10.1038/s41598-025-16210-2","url":null,"abstract":"<p><p>Mineral oil has been widely used as a liquid dielectric in transformers, serving as both an insulating and heat dissipation medium. However, its poor biodegradability, low fire resistance, and environmental concerns limit its long-term viability. To address these challenges, this study investigates natural ester oil (NEO) as an eco-friendly, biodegradable alternative, offering enhanced insulation and thermal performance. The research focuses on evaluating NEO's key characteristics, aging behavior, and failure probability, with and without Al₂O₃ nanoparticle enhancement. A 60-day accelerated thermal aging test at 100 °C was conducted to assess the degradation resistance of NEO compared to mineral oil. The effects of partial discharge, creepage failure, and electrical stress were analyzed, and a Weibull statistical model was applied to predict insulation failure probabilities. Experimental results confirm that NEO demonstrates superior breakdown strength, lower degradation rate, and higher fire safety. The addition of Al₂O₃ nanoparticles significantly improves dielectric properties, reduces viscosity, and enhances the thermal stability of NEO, making it more suitable for transformer applications. This research establishes nano-enhanced NEO as a superior alternative to mineral oil, offering higher insulation performance, improved thermal durability, and enhanced fire safety. The findings advocate for its adoption as a sustainable, high-performance dielectric for modern transformers, promoting both reliability and environmental compliance.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36311"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TET2 repression contributes to EGFR TKI resistance in EGFR-mutant non-small cell lung cancer through regulating AXIN2 methylation.","authors":"Hangjie Ying, Yamei Chen, Wei Lin, Yun Fan, Ming Chen, Ying Jin","doi":"10.1038/s41598-025-20263-8","DOIUrl":"https://doi.org/10.1038/s41598-025-20263-8","url":null,"abstract":"<p><p>Through targeted next-generation sequencing of 83 non-small cell lung cancer (NSCLC) patients with first-generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR TKI) resistance, we detected 11% TET2 mutations in the T790M-negative subgroup. To explore the molecular mechanism of TET2 in EGFR TKI resistance, reduced representation bisulfite sequencing (RRBS) was adopted to analyze the global genomic methylation profiles and detect the differentially methylated genes in the TET2-knockdown (KD) PC9 and control PC9 cell lines, following bioinformatics analysis of gene ontology (GO) functions and kyoto encyclopedia of genes and genomes (KEGG) signaling to screen for genes associated with drug resistance. TET2 KD attenuated gefitinib-induced apoptosis and decreased the sensitivity of EGFR-mutant lung cancer cells to gefitinib. Forty-three drug resistance genes with hypermethylated promoter regions were identified via RRBS and bioinformatic analysis in PC9^{TET2 KD} cells. Then, 10 candidate genes were screened for further validation. RT‒PCR demonstrated that the expression of AXIN2 and CSK was significantly lower in PC9^{TET2 KD} cells than in control cells. Furthermore, AXIN2 KD attenuated gefitinib-induced apoptosis and decreased the sensitivity of PC9 cells to gefitinib. Importantly, we found that the demethylation drug decitabine (DCA) could reverse gefitinib resistance in PC9^{TET2 KD} cells and mouse models. These results indicate that the methylation of AXIN2 induced by TET2 repression is a novel resistance mechanism of EGFR TKIs in EGFR-mutant NSCLC. Demethylation drugs have the potential to overcome EGFR TKI resistance induced by loss-of-function TET2 mutations.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36324"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validating a remote saliva collection tool for genomic analyses in free ranging dogs.","authors":"Giulia Cimarelli, Martina Lazzaroni, Clément Car, Ikhlass El Berbri, Sarah Marshall-Pescini, Małgorzata Pilot","doi":"10.1038/s41598-025-19267-1","DOIUrl":"https://doi.org/10.1038/s41598-025-19267-1","url":null,"abstract":"<p><p>Saliva is a well-established source of DNA for various applications due to its non-invasive collection and its provision of high-quality DNA. However, its use in wild and free-ranging animal research remains limited due to challenges in collection without direct animal handling. In this study, we developed and evaluated a hands-off saliva collection method designed for free-ranging domestic dogs (FRDs), serving as a model for non-invasive genetic sampling of wildlife. Our method utilized a funnel paired with a commercially available Performagene kit (DNA Genotek, Canada), presented to the dog in the presence of an operator. The dog was free to approach and interact with the apparatus, depositing saliva while trying to reach bait. We compared DNA yield and genotyping success from samples using this hands-off method with those collected via the manufacturer's recommended method. We collected 461 saliva samples from 326 FRDs, performing 750 DNA extractions. Samples collected by hand yielded significantly higher DNA concentrations after the first extraction attempt (mean = 46.3 ng/µL) than those collected using the hands-off method (mean = 32.2 ng/µL). Despite lower DNA concentrations, genotyping success did not significantly differ between methods, demonstrating that the hands-off method can yield DNA suitable for genomic analyses. The hands-off saliva collection method is a viable alternative to invasive sampling, addressing ethical concerns and enabling genomic studies in wild animals. Furthermore, our method mitigates sampling bias toward bold individuals, a common limitation in behavioral and genetic studies of free-ranging animals. With minor adaptations, this method could be applied across various species, including more elusive ones, contributing to conservation genetics and behavioral ecology research.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36315"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Continuous-wave and mode-locked Bi-doped fiber laser at 1.7 μm.","authors":"Ali Roohforouz, M R K Soltanian, Pin Long, Nitika Vaish, Lawrence R Chen","doi":"10.1038/s41598-025-20559-9","DOIUrl":"https://doi.org/10.1038/s41598-025-20559-9","url":null,"abstract":"<p><p>We investigate continuous-wave (CW) and mode-locked (ML) operation of bismuth-doped fiber (BDF) lasers at 1.7 μm. We compare the slope efficiency (SE) of the Figure-of-9 (Fo9) and linear cavity configurations for CW operation and find that the linear cavity exhibits a slightly higher SE. We demonstrate that the Fo9 configuration can also support mode-locked operation. We observe multi-pulse operation and can control the number of output pulses by adjusting the pump power.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36455"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogeochemical assessment of groundwater for agricultural suitability in the Ksour Mountains, Algeria.","authors":"Bellal Hamma, Mohamed Faouzi Bekkouche, Foued Bouaicha, Khaled A Elnagdy, Ali Alzaed, Ayoub Barkat, Antonio Randazzo, Mekonnen Sileshi Andualem, Enas E Hussein","doi":"10.1038/s41598-025-20352-8","DOIUrl":"https://doi.org/10.1038/s41598-025-20352-8","url":null,"abstract":"<p><p>The Ain-Sefra region, located in south-western Algeria has an arid climate and the groundwater is a vital resource for irrigation and domestic purposes. In the absence of other resources, conventional or otherwise, groundwater remains the driving force behind the region's development. Recognizing the importance of groundwater in agriculture, this research utilized a combination of water quality indices and GIS techniques to map and evaluate the suitability of groundwater for agricultural purposes. Several physicochemical variables, such as major elements, electrical conductivity (EC), potential hydrogen (pH), temperature (T°), total dissolved solids (TDS) were measured from 43 water samples. Hydrochemical groundwater facies were Ca-Mg-SO₄-Cl, Ca-Cl, Ca-Mg-HCO₃ and Na-Cl, revealing processes of evaporation, reverse ion exchange and rock-water interaction. Additionally, the results show that the groundwater system is oversaturated with aragonite, calcite, dolomite, anhydrite, and gypsum, while halite is estimated to be undersaturated. A range of indices, such as electrical conductivity (EC), salinity, sodium absorption rate (SAR), sodium percentage (% Na), residual sodium carbonate (RSC), magnesium hazard (MH), Kelly ratio (KR), and permeability index (PI), were used to evaluate the groundwater suitability for irrigation. Although the groundwater is found to be suitable for agricultural use, salinity control measures are essential. Spatial variation maps of water quality indices reveal that groundwater from downstream part is highly mineralized, while low mineralized water observed in the western and southwestern parts.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36441"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}