Scientific Reports最新文献

{"title":"An authoritarianism-compatible text changes British attitudes towards EU immigration.","authors":"Tessa Buchanan, David J Young, James Ackland, Alan Renwick, Lee de-Wit","doi":"10.1038/s41598-025-11491-z","DOIUrl":"https://doi.org/10.1038/s41598-025-11491-z","url":null,"abstract":"<p><p>Immigration was a major point of contention for voters in 2024, a record year for elections. Here we test whether British attitudes towards immigration become more positive when participants are exposed to a framed text. In the study (n = 3067), which uses a sample sourced from YouGov that is representative for age, education, gender and politics, participants are exposed to a short text written to be compatible with moderate levels of what political psychologists call 'authoritarianism' that also incorporates factual arguments and an emotional appeal. We find that people exposed to this text as opposed to a control text or a low authoritarianism text feel they share more values with a fictitious EU immigrant, and are more positive towards EU immigration. There are also differences in overall immigration attitudes after reading the authoritarianism-compatible text relative to the control, but these are smaller, and do not differ significantly from the low authoritarianism text. These findings demonstrate that persuasion is possible on contentious issues like immigration, and are consistent with the idea that authoritarianism-compatible arguments might be particularly effective for culturally similar forms of immigration (such as EU immigration to the UK).</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"31724"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: The impact of the integrated people-centered eye care model on myopia control among children and adolescents in China.","authors":"Xingru He, Fan Yang, Ruichong Zhang, Wei He, Cui Yu, Xiangdong He, Jiayan Chen, Guanghao Qin, Sile Yu","doi":"10.1038/s41598-025-18787-0","DOIUrl":"https://doi.org/10.1038/s41598-025-18787-0","url":null,"abstract":"","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"32444"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artifact detection in fluorescence microscopy using convolutional autoencoder.","authors":"Fabian Rehn, Marlene Pils, Tuyen Bujnicki, Oliver Bannach, Dieter Willbold","doi":"10.1038/s41598-025-18943-6","DOIUrl":"https://doi.org/10.1038/s41598-025-18943-6","url":null,"abstract":"<p><p>To ensure analytical accuracy in fluorescence microscopy image analysis, robust artifact detection is essential. For large datasets or time-sensitive analyses, automation is advisable, as it not only reduces time and costs but also eliminates human bias and enhances reproducibility. Although artificial intelligence is commonly employed for artifact detection, it is typically limited to recognizing artifact types that have been previously learned, often necessitating large training datasets. This study proposes an approach for an automated detection of previously unseen artifacts without the need for a training set of artifact-laden images. Multiple datasets were assembled using images generated by our surface-based intensity distribution analysis (sFIDA) technology during different experiments. A convolutional autoencoder was trained on a dataset of artifact-free images to reproduce preprocessed images accurately. Artifact-laden images are subsequently detected by computing the difference between the input and output of the model, with increased discrepancies indicating the presence of artifacts. The proposed model is capable of classifying artifacts across different datasets with an average accuracy of 95.5%. Additionally, the model was able to detect unseen artifacts of various types, including differences in cause, structure, size and intensity. The findings demonstrate that convolutional autoencoders provide a lightweight, but effective method for detecting artifact-laden images. While the method was tested only on sFIDA images, its design, which does not rely on an artifact specific training set, suggests potential for use across various microscopy techniques.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"32482"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-related differences in muscular coordination while performing elbow flexion and extension movements at different velocities.","authors":"Elisa Romero Avila, Hannah Lena Siebers, L Cornelius Bollheimer, Catherine Disselhorst-Klug","doi":"10.1038/s41598-025-19126-z","DOIUrl":"https://doi.org/10.1038/s41598-025-19126-z","url":null,"abstract":"<p><p>Life expectancy is increasing worldwide, with older adults expected to account for 16% of the population within 30 years. Understanding age-related declines in muscle function is crucial for prevention and maintaining independence in Activities of Daily Living (ADL). Additionally, a major consequence of these declines is the development of the physical frailty phenotype. Therefore, this study aimed to identify changes in muscular coordination during elbow flexion and extension with aging and varying movement velocities. Twelve older adults (76 ± 6.5 years) performed the movements at varying angular velocities using a constant 1 kg external load (0.4 Nm). Elbow joint kinematics and muscle activity (biceps brachii, brachioradialis, and triceps brachii) were recorded using motion capture and surface electromyography and compared to 15 healthy young adults (26.2 ± 3.2 years) under identical conditions. Clinical assessments in the older group also evaluated comorbidities, ADL dependency, and frailty risk. Despite being considered healthy, older adults showed indicators of physical decline and exhibited increased muscular coactivation, possibly as a compensatory mechanism to maintain joint stability. However, coordination patterns remained unchanged across velocities, indicating stability with age. These findings suggest changes may occur before clinical decline and should be considered in training strategies for older adults.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"32486"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial synergistic behaviour of phytosynthesized magnesium oxide nanoparticles with clove essential oil.","authors":"Asmaa O Manaa, Hoda H Baghdadi, Lamia A Heikal, Lobna S El-Hosseiny","doi":"10.1038/s41598-025-18967-y","DOIUrl":"https://doi.org/10.1038/s41598-025-18967-y","url":null,"abstract":"<p><p>While researchers continue to search for new antibacterial agents, combination therapy as well as nanotechnology-based treatments allure as promising approaches to tackle antibiotic resistance. The present study aimed to phytosynthesize magnesium oxide NPs (MgO NPs and evaluate their potential antibacterial synergistic behaviour with different essential oils (EOs). MgO NPs phytosynthesized using thyme aqueous extract were quasi-spherical with an average size of 55.2 ± 12.8 nm and an elemental composition of 35.39% Mg and 51.07% O, as determined by SEM-EDX. FTIR elicited characteristic functional group peaks, while XRD confirmed their cubic crystal structure. The phytosynthesized MgO NPs and four EOs displayed variable antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa. The checkerboard assay revealed that only clove and thyme EOs showed synergistic effects in combination with MgO NPs. Notably, MgO NPs-clove EO combination caused significant bacterial membrane damage as compared to their single counterparts in both S. aureus and E. coli. Moreover, oxidative stress was induced, as observed by the significant increase in the antioxidant enzyme activities (superoxide dismutase and catalase). Conclusively, the present results provide insights into the promising compounding of green-synthesized MgO NPs and clove EO as a bio-efficacious and eco-friendly approach to curtail antibiotic resistance.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"32484"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of nighttime odor exposure and delivery methods on subjective sleep quality in healthy adults.","authors":"Zetian Li, Jean Christoph Gerami-Manesch, Lisa Marie Hoehnel, Jonathan Warr, Antje Haehner, Thomas Hummel","doi":"10.1038/s41598-025-18075-x","DOIUrl":"https://doi.org/10.1038/s41598-025-18075-x","url":null,"abstract":"<p><p>The role of smell in improving sleep quality is inconclusive. This study aimed to investigate whether the type of odor and the method of application affect sleep quality. Based on individual preference 112 healthy participants (mean age ± SD = 31 ± 12 years, 43 men) selected an odor (lavender, orange, specifically designed \"perfume\") and an application method (odorized nasal clips, odors on pillow, odors through diffuser). Their performance was compared to a control group (n = 19; mean age ± SD = 29 ± 6 years, 9 men). Except for the control group, all groups slept with exposure of odor for 2 weeks (phase 1), followed by 2 more weeks of normal sleep without odors (phase 2). The Pittsburgh Sleep Quality Index (PSQI) and the wearable sleep monitoring device \"Fitbit Charge 2\" were used to measure sleep quality both subjectively and objectively. After adjusting for baseline differences, participants exposed to \"perfume\" reported better subjective sleep quality during the odor exposure phase compared to the control group. Among the application evaluated, the use of a diffuser was associated with better subjective sleep quality and reduced daytime fatigue, as reflected in the PSQI components. No significant group differences were observed in objective sleep parameters measured by Fitbit. Exposure to odors during the night influence sleep quality. Using a diffuser to deliver the odor appears to be associated with better subjective sleep quality and reduced daytime fatigue. No sustained improvement in sleep quality over time was observed with odor exposure.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"32452"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SDC2 and FN as cargo proteins in circulating extracellular vesicles in obese breast cancer patients with lymph node metastasis.","authors":"Liali Yousef Talat, Ghada Mohamed, Maher H Ibraheem, Amr Ahmed WalyEldeen, Hebatallah Hassan, Sherif Abdelaziz Ibrahim","doi":"10.1038/s41598-025-17638-2","DOIUrl":"https://doi.org/10.1038/s41598-025-17638-2","url":null,"abstract":"<p><p>Lymph node metastasis (LNM) is a pivotal determinant of breast cancer (BC) patient prognosis and treatment efficacy. Cell surface heparan sulfate proteoglycans (HSPGs), namely, syndecan-1 (SDC1), SDC2, and SDC4, are involved in cancer progression, metastasis, and regulate extracellular vesicles (EVs) biogenesis, including the microvesicles (MVs). This study analyzed MV-enriched EVs isolated from blood plasma of BC patients with negative (n = 19) and positive (n = 20) LNM (nLNM and pLNM, respectively) using differential centrifugation. Western blot analysis revealed significantly elevated SDC2 levels in MV-enriched EVs from pLNM cases compared to nLNM. Additionally, fibronectin (FN), a SDC2-interacting protein identified through STRING analysis, was also upregulated in pLNM MV-enriched EVs. In contrast, qRT-PCR showed reduced SDC2 (P < 0.01) and FN (P < 0.05) mRNA levels in tumor tissues of pLNM patients compared to nLNM. ROC analysis highlighted the diagnostic value of SDC2 (AUC: 0.8376) and FN (AUC: 0.8803) mRNA in differentiating LNM status. Bioinformatics analyses further confirmed the association of SDC2 and FN expression with BC staging and prognosis. These findings underscore the potential of circulating MV-enriched EV-associated SDC2 and FN, along with their tumor tissue mRNA expression, as potential predictive biomarkers for LNM and chemotherapy response in chemotherapy-naïve obese BC patients.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"32498"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Honeybee associated Aspergillus niger AW17 as a source of selective anticancer compounds with cytotoxicity evaluation in human cancer cell lines.","authors":"Hamada A Zina, Mohamed H Kalaba, Abdelghany S Shaban, Ahmed A Elrefaey, Hesham M Mahdy, Abdullah Haikal","doi":"10.1038/s41598-025-18565-y","DOIUrl":"https://doi.org/10.1038/s41598-025-18565-y","url":null,"abstract":"<p><p>Cancer remains a leading cause of death worldwide, necessitating the discovery of novel therapeutic agents with improved efficacy and selectivity. This study investigated the anticancer potential of Aspergillus niger strain AW17 isolated from honeybees, a previously unexplored ecological niche for anticancer drug discovery. The fungal isolate was characterized through morphological, microscopic, and molecular methods, confirming its identity as Aspergillus niger. Chemical profiling using GC-MS and UPLC-MS/MS revealed a complex composition including high oleic acid content (28.88%) and unique compounds like pachymic acid (18.93%), rarely associated with A. niger. The extract's anticancer activity was evaluated against colorectal (Caco-2), liver (HepG2), breast (MCF-7), and lung (A549) cancer cell lines using MTT assay, confocal microscopy with AO/PI staining, cell cycle analysis, and apoptosis detection. Results demonstrated remarkable selective cytotoxicity of the Aspergillus niger extract against cancer cell lines, following the sensitivity hierarchy HepG2 (IC₅₀ = 5.22 µg/ml) > Caco-2 (26.78 µg/ml) > A549 (34.18 µg/ml) > MCF-7 (55.91 µg/ml), while sparing normal cell lines significantly, with IC₅₀ values of 1454.7 µg/ml for WI-38 and 668.3 µg/ml for HFB-4, indicating high cancer cell selectivity. Confocal microscopy confirmed membrane integrity loss as a primary cytotoxic mechanism. Cell cycle analysis revealed cell-type specific arrest patterns, with Caco-2 and HepG2 exhibiting G1 arrest, while MCF-7 and A549 showed S phase accumulation. Flow cytometry indicated distinct death mechanisms, with Caco-2 undergoing early apoptosis and substantial necrosis, HepG2 showing predominant late apoptosis, and A549 displaying primary necrotic death. These findings highlight the potential of honeybee-associated fungi as sources of novel anticancer compounds with remarkable selectivity, providing new avenues for targeted cancer therapy development and demonstrating the value of exploring specialized ecological niches in natural product discovery.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"32472"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green hydrogen generation using mushroom-like shape bismuth molybdate-bismuth oxide/poly(1 H-pyrrole) core-shell nanocomposite from sanitation water.","authors":"Fatemah H Alkallas, Amira Ben Gouider Trabelsi, K S Almugren, Mohamed Rabia","doi":"10.1038/s41598-025-16333-6","DOIUrl":"https://doi.org/10.1038/s41598-025-16333-6","url":null,"abstract":"<p><p>A novel Bi₂MoO₆-Bi₂O₃/P1HP core-shell (C-S) nanocomposite photocathode has been successfully synthesized using a two-step process, resulting in a unique mushroom-like morphology with rough, agglomerated structures (~ 200 nm). XRD analysis confirms its nanoscale crystallite size (~ 35 nm), while optical studies reveal broad absorption extending from the visible to infrared range, with an optimized bandgap of 1.75 eV. The photocathode demonstrates exceptional hydrogen production efficiency when applied for H₂ generation using sanitation water as an electrolyte. A high hydrogen evolution rate of 2.5 µmol h^-1 cm^-2 is achieved, with current density (J_ph) measurements confirming its strong performance under various lighting conditions. Under full-spectrum white light, J_ph reaches - 0.45 mA/cm², while at 340 nm, it remains stable at -0.42 mA/cm², indicating consistent activity across different wavelengths. These findings highlight the dual benefits of this nanocomposite: efficient, eco-friendly hydrogen production while repurposing wastewater. With its broad optical absorption, cost-effective fabrication, and high photocatalytic efficiency, this innovative photocathode emerges as a promising solution for sustainable hydrogen generation.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"32434"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of highly fluorescent green carbon quantum dots from Prunus armeniaca for the determination of lisinopril in human plasma.","authors":"Baher I Salman","doi":"10.1038/s41598-025-17535-8","DOIUrl":"https://doi.org/10.1038/s41598-025-17535-8","url":null,"abstract":"<p><p>The environmentally friendly synthesis of carbon quantum dots (CQDs) using natural precursors is garnering considerable interest. Nonetheless, questions may arise concerning the properties and efficacy of CQDs obtained from identical natural sources when originating from different geographical or botanical contexts. In this study, juice extracted from Prunus armeniaca (apricots) was employed as a natural precursor to produce N@CQDs using a one-step microwave-assisted technique. The photoluminescent properties of the synthesized nitrogen-doped carbon quantum dots (N@CQDs) demonstrated significant superiority over previously reported quantum dots, achieved through a more environmentally friendly and cost-effective synthesis method. The quantum yield of these N@CQDs reached an impressive 37.1%, while their nanoscale dimensions (approximately 2.6 nm) and chemical composition remained consistent with those reported in earlier studies. Subsequently, these nanoprobes were evaluated for their ability to detect the antihypertensive drug lisinopril (LIS). The results indicated that the N@CQDs exhibited both selectivity and sensitivity for LIS detection in bulk powder and biological plasma samples, within a concentration range of 5.0-150.0 ng mL^- 1. The lower limit of quantitation was determined to be 2.2 ng mL^- 1. The presence of LIS resulted in a significant reduction in the luminescence intensity of the synthesized green and stable N@CQDs at 502 nm (with an excitation wavelength of 455 nm). Additionally, the reusability and stability of the proposed CQDs for LIS analysis were thoroughly validated.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"32502"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}