Scientific Reports最新文献

{"title":"Deceleration capacity derived from a five-minute electrocardiogram predicts mortality in the general population.","authors":"Alexander Steger, Petra Barthel, Alexander Müller, Ina-Maria Rückert-Eheberg, Birgit Linkohr, Julia Allescher, Melanie Maier, Alexander Hapfelmeier, Eimo Martens, Helene Hildegard Heidegger, Arne Michael Müller, Konstantinos D Rizas, Stefan Kääb, Moritz F Sinner, Daniel Sinnecker, Karl-Ludwig Laugwitz, Annette Peters, Georg Schmidt","doi":"10.1038/s41598-024-83712-w","DOIUrl":"https://doi.org/10.1038/s41598-024-83712-w","url":null,"abstract":"<p><p>In contemporary healthcare, effective risk stratification in the general population is vital amidst rising chronic disease rates and an ageing demographic. Deceleration Capacity of the heart rate (DC), derived from 24-hour Holter electrocardiograms, holds promise in risk stratification for cardiac patients. However, the potential of short-term electrocardiograms of five minutes duration for population screening has not been fully explored. Our study aims to investigate the utility of Deceleration Capacity derived from short-term electrocardiograms as a scalable, fully-automated screening tool for predicting long-term mortality risk in the general population. Within a cohort of a representative population-based survey in Germany (KORA-KMC-study), 823 participants with sinus rhythm aged 27 to 76 years at enrollment (females 47.4%) were followed for a median of 13.4 years (IQR 13.1-13.6). All-cause mortality was defined as the primary endpoint and observed in 159 participants. Deceleration Capacity was calculated from 5-minute 12-lead electrocardiograms by a fully automated approach. Participants were divided into three predefined risk categories: DC_category0 - low-risk (> 4.5ms); DC_category1 - intermediate-risk (2.5-4.5ms); and DC_category2 - high-risk (≤ 2.5ms). More than two-thirds of the participants (n = 564, 68.5%) fell into DC_category0, about one-fifth (n = 168, 20.4%) into DC_category1, and about one-tenth (n = 91, 11.1%) into DC_category2. Estimated 13-years mortality in the risk groups was 16.7%, 23.5%, and 49.1%, respectively (p < 0.001). Adjusting for age, life-style-related risk factors, and comorbidities, increased mortality risk was observed for DC_category2 (HR 2.34, 95%-CI 1.56-3.50). Deceleration Capacity, derived automatically from brief 5-minute electrocardiogram recordings, emerges as a robust, feasible, and independent predictor of long-term mortality risk in the general population.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30566"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testing perioperative meloxicam analgesia to enhance welfare while preserving model validity in an inflammation-induced seizure model.","authors":"Edna Weiß, Alberto Pauletti, Asya Egilmez, Sonja Bröer","doi":"10.1038/s41598-024-81925-7","DOIUrl":"https://doi.org/10.1038/s41598-024-81925-7","url":null,"abstract":"<p><p>Despite the international effort to improve laboratory animal welfare through the 3R principles (Reduce, Refine, Replace), many scientists still fail to implement and report their assessment of pain and well-being, likely due to concerns regarding the potential effects of analgesics on experimental outcomes. This study aimed to determine whether refining our viral encephalitis model with perioperative analgesia could enhance well-being and recovery after intracerebral virus infection without impacting disease outcomes. We routinely use the Theiler's Murine Encephalomyelitis Virus (TMEV) model to study virus-induced epilepsy. Given the crucial role of immune cell activation in acute seizure development, we evaluated the effects of the non-steroidal anti-inflammatory drug (NSAID) meloxicam on inflammation, neurodegeneration, and neuronal cell proliferation at 7 days post-infection (dpi). Overall, the impact of virus infection on well-being was less severe than anticipated, and meloxicam treatment did not affect well-being or nest building behavior in TMEV-infected mice. Furthermore, meloxicam treatment did not influence key experimental readouts such as seizure burden, central inflammatory response, neurodegeneration, or neuronal proliferation within the hippocampus. Notably, animals experiencing seizures displayed heightened inflammatory responses and neurodegeneration, which were not influenced by meloxicam treatment. In summary, perioperative analgesia did not compromise key outcome measures such as seizure frequency, inflammation, and neurodegeneration or -regeneration in the TMEV model. However, it also did not add any significant benefits to well-being in the first week after intracranial injections.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30563"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable zinc alloys with high strength and suitable mechanical integrity as bone repair metals.","authors":"Chengwu Lu, Chao Song, Yunlong Yu, Linhai Yang, Wu Zheng, Fenqi Luo, Yuhua Xiao, Jun Luo, Jie Xu","doi":"10.1038/s41598-024-78842-0","DOIUrl":"https://doi.org/10.1038/s41598-024-78842-0","url":null,"abstract":"<p><p>Mechanical properties and integrity of biodegradable Zn alloys during degradation holds significant importance. In this study, a Zn-Mg-Mn alloy with tensile strength of 414 MPa and an elongation of 26% was developed. The strength contributions of as-extruded Zn alloy from grain boundary strengthening, precipitation strengthening, and second phase strengthening. Degradation of the Zn alloy in Hank's solution exhibited a decreasing trend with prolonged immersion, eventually stabilizing at 16 μm/year. Corrosion morphology analysis revealed that the corrosion modes transformed from pitting corrosion to severely localized corrosion with prolonged immersion time, eventually lead to formation of large holes. Although the tensile strength of the Zn alloys remained relatively unchanged following varied immersion time, a substantial decrease in elongation was observed. The decreased elongation primarily attributed to the formation of surface corrosion pits or holes, exacerbating crack propagation during tension. Biocompatibility assessments of Zn alloys demonstrated that a 50% concentration of Zn alloy leach solution cultured with C3H10 and RMSC cells yielded cellular activity exceeding 80%, indicating excellent cytocompatibility. Alkaline phosphatase (ALP) and alizarin red staining results further underscored the remarkable early and late osteogenic properties exhibited by Zn-Mg-Mn alloy.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30558"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term effects of adding biochar to soils on organic matter content, persistent carbon storage, and moisture content in Karagwe, Tanzania.","authors":"Baraka Ernest, Pius Z Yanda, Anders Hansson, Mathias Fridahl","doi":"10.1038/s41598-024-83372-w","DOIUrl":"https://doi.org/10.1038/s41598-024-83372-w","url":null,"abstract":"<p><p>Soils require the application of biochar to improve degradation. The objective of this study was to evaluate the long-term effects of a field experiment on soil organic matter (SOM), soil organic carbon (SOC), and soil moisture content in Karagwe, Tanzania. Seven years ago, a field experiment was conducted using a Latin rectangle design with four replications. The treatments included carbonization and sanitation (CaSa) and carbonization and standard compost (CaSt), which were compared to control Andosols (CoA). A total of 96 soil samples were collected to determine SOM, SOC, and soil moisture content. The data were analyzed using one-way analysis of variance. The results showed that soil samples from the CaSa-treated soil had an increase in SOM content of 17.3%, an increase in stored SOC content of 10.0%, and an increase in soil moisture content of 6.3%. Compared with those in CoA, the CaSt-treated soil showed increases in SOM, SOC, and soil moisture of 14.4%, 8.4%, and 4.0%, respectively. Therefore, all treatments improved soil properties, with CaSa proving more effective in enhancing SOM, SOC, and soil moisture content compared to CaSt and CoA. In conclusion, CaSa is recommended for its sustainable ability to enhance Karagwean soil over time.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30565"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author Correction: Spatiotemporal dynamic assessment and obstacle analysis of economic resilience in China's marine fisheries.","authors":"Shou-Yi Qiao, Yi-Pin Fan, Wei Yin","doi":"10.1038/s41598-024-82997-1","DOIUrl":"https://doi.org/10.1038/s41598-024-82997-1","url":null,"abstract":"","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30567"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Publisher Correction: Development and characterization of a self-nano emulsifying drug delivery system (SNEDDS) for Ornidazole to improve solubility and oral bioavailability of BCS class II drugs.","authors":"Popat Mohite, Shruti Sule, Anil Pawar, Hanan M Alharbi, Swastika Maitra, Vetriselvan Subramaniyan, Vinoth Kumarasamy, Daniel Ejim Uti, Celestine O Ogbu, Simon Inedu Oodo, Ajoy Kumer, Ayodeji Oluwafemi Idowu, Okechukwu N N Okoye","doi":"10.1038/s41598-024-82249-2","DOIUrl":"https://doi.org/10.1038/s41598-024-82249-2","url":null,"abstract":"","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30556"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transferable deep learning with coati optimization algorithm based mitotic nuclei segmentation and classification model.","authors":"Amal Alshardan, Nazir Ahmad, Achraf Ben Miled, Asma Alshuhail, Yazeed Alzahrani, Ahmed Mahmud","doi":"10.1038/s41598-024-80002-3","DOIUrl":"https://doi.org/10.1038/s41598-024-80002-3","url":null,"abstract":"<p><p>Image processing and pattern recognition methods have recently been extensively implemented in histopathological images (HIs). These computer-aided techniques are aimed at detecting the attentive biological markers for assisting the final cancer grading. Mitotic count (MC) is a significant cancer detection and grading parameter. Conventionally, a pathologist examines the biopsy image physically by employing higher-power microscopy. The MC cells have been marked physically at every analysis, and total MC must be utilized as a major aspect for the cancer ranking and considered as the initiative of cancers. Numerous pattern recognition algorithms for cell-sized objects in HIs depend upon segmentation to assess features. The correct description of the segmentation has been difficult, and feature outcomes can be highly complex to the segmentation. The MC cells are an essential element in many cancer grading methods. Extraction of the MC cell from the HI is a highly challenging assignment. This manuscript proposes the Coati Optimization Algorithm with Deep Learning-Driven Mitotic Nuclei Segmentation and Classification (COADL-MNSC) technique. The major aim of the COADL-MNSC technique is to utilize the DL model to segment and classify the mitotic nuclei (MN). In the preliminary stage, the COADL-MNSC approach implements median filtering (MF) for pre-processing. Besides, the COADL-MNSC approach utilizes the Hybrid Attention Fusion U-Net (HAU-UNet) model to segment the MN. Moreover, the capsule network (CapsNet) model is employed for the feature extraction method, and its hyperparameters are adjusted by utilizing the COA model. At last, the classification procedure is performed using the bidirectional long short-term memory (BiLSTM) model. Extensive simulations are performed under the MN image dataset to exhibit the excellent performance of the COADL-MNSC methodology. The experimental validation of the COADL-MNSC methodology portrayed a superior accuracy value of 98.89% over existing techniques under diverse measures.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30557"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author Correction: Frequency and spectrum of mutations in human sperm measured using duplex sequencing correlate with trio-based de novo mutation analyses.","authors":"Jonatan Axelsson, Danielle LeBlanc, Habiballah Shojaeisaadi, Matthew J Meier, Devon M Fitzgerald, Daniela Nachmanson, Jedidiah Carlson, Alexandra Golubeva, Jake Higgins, Thomas Smith, Fang Yin Lo, Richard Pilsner, Andrew Williams, Jesse Salk, Francesco Marchetti, Carole Yauk","doi":"10.1038/s41598-024-82618-x","DOIUrl":"https://doi.org/10.1038/s41598-024-82618-x","url":null,"abstract":"","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30571"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histone variant H3.5 in testicular cell differentiation and its interactions with histone chaperones.","authors":"Patrick Philipp Weil, Anton Pembaur, Beatrice Wirth, Eda Oetjen, Hannes Büsscher, Klemens Zirngibl, Malte Czarnetzki, Stella Braun, Jann-Frederik Cremers, Daniel Gödde, Stephan Degener, Jan Postberg","doi":"10.1038/s41598-024-83206-9","DOIUrl":"https://doi.org/10.1038/s41598-024-83206-9","url":null,"abstract":"<p><p>Testicular cell differentiation is a highly regulated process, essential for male reproductive health. The histone variant H3.5 is apparently a critical player in this intricate orchestra of cell types, but its regulation and function remains poorly understood. To elucidate its role, we fractionized testicular cells using c-Kit/CD117 as a separation marker and analyzed H3.5 expression. Further, we investigated the regulation of H3.5 expression using public data repositories. We explored DNA methylation patterns in specific regions of the H3-5 gene and assessed H3-5 copy number gain in seminoma specimens. Additionally, we examined the testicular localization of H3.5 and its histone chaperone interactions to understand its regulation at the protein level. We used qRT-PCR, MeDIP, and qPCR to study H3.5 expression and DNA methylation in various cell types. H3-5 copy number gain was analyzed using qPCR. Protein interactions were investigated through fluorescence-2-hybrid assays in baby hamster kidney cells. H3.5 is primarily enriched in spermatocytes. DNA methylation of a CpG island overlapping the H3-5 promoter appeared to be involved in the tissue-specific regulation of H3.5 expression. Elevated H3.5 expression was observed in seminoma specimens, suggesting a potential link to testicular tumors. H3-5 copy number gain was associated with elevated H3.5 expression in seminoma specimens. Furthermore, we identified physical interactions between H3.5 and histone chaperones Asf1a and Asf1b, HIRA, CAF p150 and DAXX, shedding light on the protein-level regulation of H3.5. These findings provide valuable insights into the molecular mechanisms governing testicular cell differentiation and the potential role of H3.5 in testicular pathologies.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30564"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Publisher Correction: Web log mining techniques to optimize Apriori association rule algorithm in sports data information management.","authors":"Tiantian Li, Fang Liu, Xiaobin Chen, Chao Ma","doi":"10.1038/s41598-024-82236-7","DOIUrl":"https://doi.org/10.1038/s41598-024-82236-7","url":null,"abstract":"","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"30568"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}