Scientific Reports最新文献

{"title":"The resolvin D2 and omega-3 polyunsaturated fatty acid as a new possible therapeutic approach for inflammatory bowel diseases.","authors":"Fabio Henrique Mendonça Chaim, Lívia Bitencourt Pascoal, Marina Moreira de Castro, Bruna Biazon Palma, Bruno Lima Rodrigues, João José Fagundes, Marciane Milanski, Luiz Roberto Lopes, Raquel Franco Leal","doi":"10.1038/s41598-024-80051-8","DOIUrl":"https://doi.org/10.1038/s41598-024-80051-8","url":null,"abstract":"<p><p>Inflammatory bowel diseases (IBD) are idiopathic disorders characterized by chronic gastrointestinal inflammation. Given conventional therapies' adverse effects and clinical failures, novel approaches are being investigated. Recent studies have highlighted the role of specialized pro-resolving lipid mediators (SPMs) in the active resolution of chronic inflammation. In this regard, omega-3 fatty acid-derived Resolvin D2 (RvD2) appears to play a protective role in the pathophysiology of IBD. Therefore, we characterized the RvD2 pathway and its receptor expression in the intestinal mucosa of experimental colitis induced by dextran sulfate sodium. We also evaluated the preventive impact of an omega-3-enriched diet and the therapeutic efficacy of RvD2 compared with anti-TNF-α treatment. We found an increase in TNFα and IL22 expression and decreased levels of enzymes involved in RvD2 biosynthesis, such as PLA₂, 15-LOX, 5-LOX, and its receptor GPR18 in experimental colitis. Omega-3 supplementation reduced the Disease Activity Index (DAI), weight loss, colonic shortening, and inflammation. These results and the increased IL-10 transcriptional levels after RvD2 treatment suggest that this mediator attenuated experimental colitis. These results enhance our understanding of the molecular mechanisms involved in the exacerbated inflammatory response present in experimental colitis and suggest that RvD2 and its omega-3 precursor offer a promising therapeutic approach for IBD.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"28698"},"PeriodicalIF":3.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of key chlorophyll fluorescence parameters as biomarkers for dryland wheat under future climate conditions.","authors":"Ramin Lotfi, Farzaneh Eslami-Senoukesh, Arash Mohammadzadeh, Esmail Zadhasan, Amin Abbasi, Hazem M Kalaji","doi":"10.1038/s41598-024-80164-0","DOIUrl":"https://doi.org/10.1038/s41598-024-80164-0","url":null,"abstract":"<p><p>Nowadays, climate change is the primary factor shaping the future of food and nutritional security. To investigate the interactive effects of various climate variables on photosynthetic efficiency, an experiment was conducted using 10 dryland wheat genotypes. These genotypes were exposed to different conditions: temperatures of 25 ± 3 °C and 34 ± 3 °C, carbon dioxide concentrations of 380 ± 50 ppm and 800 ± 50 ppm, and irrigation regimes of 50% field capacity and well-watered. Our results indicated that the wheat genotypes responded differently to both individual and combined climate stress factors. The traditional winter wheat genotype *Sardari*, along with the newly developed dryland wheat genotype *Ivan*, exhibited resilience to anticipated climate conditions. This resilience was reflected in enhancements in photochemical quantum efficiency parameters (Y(II), qP, and qL) under combined stress conditions. Resilient genotypes demonstrated superior regulation of the stomatal conductance (GS) and electron transport rate (ETR) under elevated temperature and CO₂ levels. Principal component analysis (PCA) revealed significant correlations between chlorophyll fluorescence parameters and climate factors, such as NPQ with temperature, Y(NO) with CO₂, qL in response to drought stress, and both qP and Y(II) with the interactions among temperature, CO₂, and drought stress. Elevated CO₂ reduced the ETR and GS across all genotypes. Our findings underscore the importance of assessing not only fundamental chlorophyll fluorescence parameters like Fm and Fo but also the efficiency of NPQ and Y(II) to understand climate change impacts on dryland wheat genotypes. We suggest that these parameters could serve as valuable biomarkers for breeding programs aimed at improving plant adaptation to future dryland climate conditions.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"28699"},"PeriodicalIF":3.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Truncating the C terminus of formate dehydrogenase leads to improved preference to nicotinamide cytosine dinucleotide.","authors":"Xiaojia Guo, Xueying Wang, Yinghan Hu, Lingyun Zhang, Zongbao K Zhao","doi":"10.1038/s41598-024-79885-z","DOIUrl":"https://doi.org/10.1038/s41598-024-79885-z","url":null,"abstract":"<p><p>Formate dehydrogenase (FDH) is widely applied in regeneration of redox cofactors. There are continuing interests to engineer FDH for improved catalytic activity and cofactor preference. In the crystal structure of FDH from Pseudomonas sp. 101 (pseFDH), the C terminus with 9 amino acid residues cannot be resolved. However, our earlier work showed mutations at C terminus led pseFDH variants to favor a non-natural cofactor nicotinamide cytosine dinucleotide (NCD). Here, we investigated the role of C-terminal residues on cofactor preference by truncating their corresponding C terminus of pseFDH variants. Sequence comparison analysis showed that C-terminal residues were barely conservative among different FDHs. pseFDH and mutants with their C termini truncated were constructed, and the resulted variants showed improved preference to NCD mainly because NAD-dependent activity dropped more substantially. Further structure analysis showed that these pseFDH variants had their cofactor binding domains reconstructed to favor molecular interactions with NCD. Our work indicated that C-terminal residues of pseFDH affected enzyme activity and cofactor preference, which provides a new approach for ameliorating the performance of redox enzymes.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"28701"},"PeriodicalIF":3.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A qualitative study exploring the clinical phenomenology and impact of hypersexuality in patients with Parkinson's Disease.","authors":"Natalie Tayim, Jalesh N Panicker, Jennifer Foley, Caroline Selai, Walaa G El Sheikh","doi":"10.1038/s41598-024-79966-z","DOIUrl":"https://doi.org/10.1038/s41598-024-79966-z","url":null,"abstract":"<p><p>Hypersexuality (HS) is a complex yet underreported phenomenon in Parkinson's disease (PD), profoundly impacting patients' lives. This study aims to systematically investigate the clinical phenomenology and manifestations of HS using qualitative methodologies. This phenomenological qualitative research study employed semi-structured interviews to examine hypersexuality and its impact among nine English-speaking individuals with PD. Participants were recruited from clinical settings and met specific inclusion criteria, including clinically diagnosed PD, reported hypersexuality post-PD diagnosis, and no cognitive impairment. The study adhered to ethical standards, with written informed consent obtained from all participants. Nine patients with PD (six males, mean age 61.7 ± 13.3 years, and three females, mean age 64.3 ± 5.7 years) participated. The mean age of PD onset was 51.4 ± 12.5 years, while HS onset was 54.1 ± 11.5 years, ranging from 35 to 68 years. Eight of the nine patients were in monogamous relationships. Qualitative analysis revealed ten themes. Clinical manifestations included increased preoccupation with sex, heightened desire, and altered behaviors like risk-taking. Sexual practices varied, with increased urges not necessarily leading to more frequent sex with partners; instead, behaviors like masturbation and promiscuity were common. Emotional formulations ranged from negative to neutral, influenced by whether patients internalized or externalized their hypersexuality. Insight varied, with some patients viewing HS as natural and others seeing it as conflicting with their values. Control over HS was mixed, with efforts to manage behaviors influenced by personal or external factors. The impact on life was predominantly negative, affecting marital closeness, family dynamics, social interactions, work efficiency, and physical health. Patients perceived mostly negative feelings from their partners regarding HS. Stigma was significant, including personal shame, social concealment, and discomfort discussing HS with health professionals. Barriers to seeking help included communication deficits, professional neglect, and stigma. Despite challenges, patients expressed a desire for better guidance and open discussions with health professionals to manage HS. This study uncovered the profound impact of HS on various facets of life such as quality of life, work, and personal relationships, elucidating the emotional distress and societal challenges faced by patients. This preliminary study on hypersexuality in neurological disorders suggests multiple avenues for future research.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"28697"},"PeriodicalIF":3.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author Correction: Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients.","authors":"Andrea Čeri, Anita Somborac-Bačura, Marija Fabijanec, Andrea Hulina-Tomašković, Marko Matusina, Dijana Detel, Donatella Verbanac, Karmela Barišić","doi":"10.1038/s41598-024-80214-7","DOIUrl":"https://doi.org/10.1038/s41598-024-80214-7","url":null,"abstract":"","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"28633"},"PeriodicalIF":3.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author Correction: Leveraging genetic resource diversity and identification of trait-enriched superior genotypes for accelerated improvement in linseed (Linum usitatissimum L.).","authors":"Vikender Kaur, Sunil S Gomashe, Shashank K Yadav, Devender Singh, Sheela, Shubhendra Singh Chauhan, Vinay Kumar, Balram Jat, Nandan Ramesh Tayade, Sapna Langyan, Nutan Kaushik, Mamta Singh, Munisha Kheralia, Dhammaprakash Pandhari Wankhede, J Aravind, Vartika Srivastava, Kavita Gupta, Ashok Kumar, Gyanendra Pratap Singh","doi":"10.1038/s41598-024-78594-x","DOIUrl":"https://doi.org/10.1038/s41598-024-78594-x","url":null,"abstract":"","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"28600"},"PeriodicalIF":3.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Daily intake of a dairy-based nutritional supplement improved self-reported gastrointestinal symptoms and modulated microbiota in adult Chinese volunteers.","authors":"Klaudyna Borewicz, Yifan Zhao, Yaqiong Zhu","doi":"10.1038/s41598-024-79360-9","DOIUrl":"https://doi.org/10.1038/s41598-024-79360-9","url":null,"abstract":"<p><p>Mild and persisting gastrointestinal symptoms (MPGS) as bloating, constipation or diarrhea are widespread in older adults and often accompanied by gut microbiota dysbiosis. Nutritional interventions help to regulate and restore microbiota and gut function. In this non-randomized continuous prospective cohort study, we evaluated the effects of a 21-day intervention using a dietary dairy-based supplement (AGIJOY™) on self-reported gastrointestinal (GI) symptoms, fecal microbiota composition and short chain fatty acids (SCFA) levels in a cohort of Chinese adults (N = 46, age 27-73) suffering from MPGS. Questionnaire data and fecal samples were collected at baseline (D0), on day 7 and 21 of intervention (D7 and D21). The results showed significant improvement in the self-reported GI symptoms on D21 and a gradual shift in the fecal microbiota composition. In the first week of intervention, the relative abundance (RA) of fecal bifidobacteria significantly increased and the RA of Bacteroides and Helicobacter decreased (p < 0.05). The levels of fecal SCFA remained unchanged during the study. To our knowledge, this is the first study testing the effect of this prebiotic and postbiotic containing milk-based supplement blend on gut microbiota and MPGS among Chinese population under a real living environment.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"28651"},"PeriodicalIF":3.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ETV2 transcriptionally activates Rig1 gene expression and promotes reprogramming of the endothelial lineage.","authors":"Young Geun Choi, Xiao Ma, Satyabrata Das, Javier E Sierra-Pagan, Thijs Larson, Wuming Gong, Hesham A Sadek, Jianyi Jay Zhang, Mary G Garry, Daniel J Garry","doi":"10.1038/s41598-024-78115-w","DOIUrl":"https://doi.org/10.1038/s41598-024-78115-w","url":null,"abstract":"<p><p>ETV2 is an essential transcription factor as Etv2 null murine embryos lack all vasculature, blood and are lethal early during embryogenesis. Previous studies have established that ETV2 functions as a pioneer factor and directly reprograms fibroblasts to endothelial cells. However, the underlying molecular mechanisms regulating this reprogramming process remain incompletely defined. In the present study, we examined the ETV2-RIG1 cascade as regulators that govern ETV2-mediated reprogramming. Mouse embryonic fibroblasts (MEFs) harboring an inducible ETV2 expression system were used to overexpress ETV2 and reprogram these somatic cells to the endothelial lineage. Single-cell RNA-seq from reprogrammed fibroblasts defined the induction of the transcriptional network involved in Rig1-like receptor signaling pathways. Studies using ChIP-seq, electrophoretic mobility shift assays, and transcriptional assays demonstrated that ETV2 was a direct upstream activator of Rig1 gene expression. We further demonstrated that the knockdown of Rig1 and separately, Nfκb1 using shRNA significantly reduced the efficiency of endothelial cell reprogramming. These results highlight that ETV2 reprograms fibroblasts to endothelial cells by directly activating RIG1. These findings extend our current understanding of the molecular mechanisms underlying ETV2-mediated reprogramming and will be important in the design of revascularization strategies for the treatment of ischemic tissues such as ischemic heart disease.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"28688"},"PeriodicalIF":3.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the cognitive underpinnings of early hominin stone tool use through an experimental EEG approach.","authors":"Simona Affinito, Brienna Eteson, Lourdes Tamayo Cáceres, Elena Theresa Moos, Fotios Alexandros Karakostis","doi":"10.1038/s41598-024-77452-0","DOIUrl":"https://doi.org/10.1038/s41598-024-77452-0","url":null,"abstract":"<p><p>Technological innovation has been crucial in the evolution of our lineage, with tool use and production linked to complex cognitive processes. While previous research has examined the cognitive demands of early stone toolmaking, the neurocognitive aspects of early hominin tool use remain largely underexplored. This study relies on electroencephalography to investigate brain activation patterns associated with two distinct early hominin tool-using behaviors: forceful hammerstone percussion, practiced by both humans and non-human primates and linked to the earliest proposed stone tool industries, and precise flake cutting, an exclusive hominin behavior typically associated with the Oldowan. Our results show increased engagement of the frontoparietal regions during both tasks. Furthermore, we observed significantly increased beta power in the frontal and centroparietal areas when manipulating a cutting flake compared to a hammerstone, and increased beta activity over contralateral frontal areas during the aiming (planning) stage of the tool-using process. This original empirical evidence suggests that certain fundamental brain changes during early hominin evolution may be linked to precise stone tool use. These results offer new insights into the complex interplay between technology and human brain evolution and encourage further research on the neurocognitive underpinnings of hominin tool use.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"26936"},"PeriodicalIF":3.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive value of triglyceride-glucose index for the occurrence of acute respiratory failure in asthmatic patients of MIMIC-IV database.","authors":"Qi Feng, ZiWen Lv, Chun Xiao Ba, Ying Qian Zhang","doi":"10.1038/s41598-024-74294-8","DOIUrl":"https://doi.org/10.1038/s41598-024-74294-8","url":null,"abstract":"<p><p>This study aims to investigate the association between the triglyceride-glucose (TyG) index and the occurrence of acute respiratory failure in asthma patients. This retrospective observational cohort study utilized data from the Medical Information Mart for Intensive Care IV (MIMIC-IV 2.2) database. The primary outcome was the development of acute respiratory failure in asthma patients. Initially, the Boruta algorithm and SHapley Additive exPansions were applied to preliminarily determine the feature importance of the TyG index, and a risk prediction model was constructed to evaluate its predictive ability. Secondly, Logistic regression proportional hazards models were employed to assess the association between the TyG index and acute respiratory failure in asthma patients. Finally, subgroup analyses were conducted for sensitivity analyses to explore the robustness of the results. A total of 751 asthma patients were included in the study. When considering the TyG index as a continuous variable, logistic regression analysis revealed that in the unadjusted Model 1, the odds ratio (OR) was 2.381 (95% CI: 1.857-3.052; P < 0.001), in Model II, the OR was 2.456 (95% CI: 1.809-3.335; P < 0.001), and in the multivariable-adjusted model, the OR was 1.444 (95% CI: 1.029-2.028; P = 0.034). A consistent association was observed between the TyG index and the risk of acute respiratory failure in asthma patients. No significant interaction was found between the TyG index and various subgroups (P > 0.05). Furthermore, machine learning results indicated that an elevated TyG index was a significant feature predictive of respiratory failure in asthma patients. The baseline risk model achieved an AUC of 0.743 (95% CI: 0.679-0.808; P < 0.05), whereas the combination of the baseline risk model with the TyG index yielded an AUC of 0.757 (95% CI: 0.694-0.821; P < 0.05). The TyG index can serve as a predictive indicator for acute respiratory failure in asthma patients, albeit confirmation of these findings requires larger-scale prospective studies.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"28631"},"PeriodicalIF":3.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}