Optimized cesarean techniques, IVF use, and foster strain selection enhance germ-free mouse production efficiency.

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-07-26 DOI:10.1038/s41598-025-05411-4

Yuting Yang, Weimin He, Jingqiang Li, Jinhui Wang, Yuyan Tang, Haitao Shang

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Abstract

This study aims to refine sterile cesarean section techniques, in vitro fertilization (IVF) and foster mother selection strategies to enhance the efficiency of germ-free (GF) mouse production. We conducted the following analyses: (1) comparing different cesarean section methods, (2) evaluating the impact of natural mating versus in vitro fertilization (IVF) for obtaining donor mice, and (3) assessing maternal care among three inbred strains (C57BL/6J, BALB/c, NSG) and one outbred strain (KM) as GF foster mothers. The results showed that optimizing surgical methods which preserve the female reproductive tract during cesarean section (FRT-CS), significantly improved fetal survival rates while maintaining sterility. IVF enabled precise control over donor delivery dates, enhancing experimental reproducibility. Among GF foster strains, BALB/c and NSG mice exhibited superior nursing and weaning success, whereas C57BL/6J had the lowest weaning rate, in stark contrast to findings on maternal care in SPF C57BL/6J foster mothers. These findings offer practical guidance for optimizing GF mouse production, improving the efficiency of obtaining germ-free mice through cesarean derivation.

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优化的剖宫产技术、体外受精的使用和培养菌株的选择提高了无菌小鼠的生产效率。

本研究旨在改进无菌剖宫产技术、体外受精（IVF）和育母选择策略，以提高无菌（GF）小鼠的生产效率。我们进行了以下分析：(1)比较不同的剖宫产方法；(2)评估自然交配与体外受精（IVF）对获得供体小鼠的影响；(3)评估3种自交系（C57BL/6J、BALB/c、NSG）和1种自交系（KM）作为GF育母的母性护理。结果表明，优化剖宫产术中保留女性生殖道的手术方法，在保持胎儿不育的同时显著提高了胎儿存活率。试管婴儿能够精确控制供体的分娩日期，提高实验的可重复性。在GF培养品系中，BALB/c和NSG小鼠的哺乳和断奶成功率较高，而C57BL/6J小鼠的断奶率最低，与SPF C57BL/6J母系的母性护理结果形成鲜明对比。这些发现对优化GF小鼠的生产，提高通过剖宫产获得无菌小鼠的效率具有实际指导意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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