Scientific Reports最新文献

{"title":"Spatial distribution and functional integration of displaced retinal ganglion cells.","authors":"Sabrina Duda, Christoph T Block, Dipti R Pradhan, Yousef Arzhangnia, Alina Klaiber, Martin Greschner, Christian Puller","doi":"10.1038/s41598-025-91045-5","DOIUrl":"10.1038/s41598-025-91045-5","url":null,"abstract":"<p><p>The retina contains distinct types of ganglion cells, which form mosaics with cells of each type at each position of the visual field. Displaced retinal ganglion cells (dRGCs) occur with cell bodies in the inner nuclear layer (INL), and regularly placed RGCs with cell bodies in the ganglion cell layer. An example of mammalian dRGCs are M1-type intrinsically photosensitive ganglion cells (ipRGCs). Little is known, however, about their relationship with regularly placed ipRGCs. We identified mouse ipRGC types M1, M2, and M4/sONɑ by immunohistochemistry and light microscopy. Reconstruction of immunolabeled mosaics from M1 and sONɑ RGCs indicated that dRGCs tiled the retina with their regular RGC partners. Multi-electrode array recordings revealed conventional receptive fields of displaced sONɑ RGCs which fit into the mosaic of their regular counterparts. An RGC distribution analysis showed type-specific dRGC patterns which followed neither the global density distribution of all RGCs nor the local densities of corresponding cell types. The displacement of RGC bodies into the INL occurs in a type-dependent manner, where dRGCs are positioned to form complete mosaics with their regular partners. Our data suggest that dRGCs and regular RGCs serve the same functional role within their corresponding population of RGCs.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"7123"},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liver fatty acid-binding protein point-of-care testing detects heat-induced organ damage: a pilot study in Japanese male self-defense force personnel.","authors":"Hiroyasu Goto, Takuya Ishikiriyama, Kyoko Oe, Reina Asaga, Hiroki Sato, Kazuma Mori, Bradley M Kearney, Hiroyuki Nakashima, Takeshi Sugaya, Manabu Kinoshita, Naoki Oshima","doi":"10.1038/s41598-025-91685-7","DOIUrl":"https://doi.org/10.1038/s41598-025-91685-7","url":null,"abstract":"<p><p>Heat-related illnesses cause multiple organ injuries, including acute kidney injury (AKI). Recent studies have reported that heat-induced AKI can progress to chronic kidney disease (CKD). We previously reported that urinary levels of liver fatty acid-binding protein (L-FABP) are elevated in patients with severe heat-related illness. In this study, we prospectively examined the detection ability of L-FABP rapid assay kit (L-FABP Point-of-Care [POC] kit) for heat-induced organ damage in prehospital settings. After applying the exclusion criteria, 65 Japanese male military personnel who intended to carry out training in a hot environment were analyzed. The L-FABP POC kit enabled the detection of heat-induced kidney and/or liver damage after heat exposure (defined as serum creatinine [Cr] ≥ 1.2 mg/dL and total bilirubin ≥ 1.2 mg/dL) with a high negative predictive value (95.7%). L-FABP-positive participants showed higher serum Cr and total bilirubin levels than L-FABP-negative participants. Moreover, L-FABP-positive participants showed higher acyl-to-free carnitine ratios, indicating carnitine insufficiency which leads to impaired fatty acid oxidation, as well as high and rapid elevation of their core temperature in comparison to L-FABP-negative participants. In conclusion, the L-FABP POC kit may be useful as a screening tool for detecting heat-induced organ damage, which would prevent prolonged organ dysfunction.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"7197"},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonequilibrium phase diagram for the serration statistics during slow deformation of refractory high-entropy alloys.","authors":"Ming-Wei Liu, Wei-Chen Hsu, Tzu-Yang Chiang, Che-Wei Tsai, Karin A Dahmen","doi":"10.1038/s41598-025-90338-z","DOIUrl":"https://doi.org/10.1038/s41598-025-90338-z","url":null,"abstract":"<p><p>Diverse slip behaviors are observed in HfNbTaTiZr refractory high-entropy alloy during tensile tests. Slow-avalanche and fast-runaway phases are identified by analyzing scaling relationships among slip statistics. Slow avalanches display a scaling collapse in temporal shapes, aligning with mean-field slip theory, while system-spanning fast-runaway avalanches show a Gaussian shape, indicating rapid nucleation due to dynamic weakening. Through time scale analysis, we construct a nonequilibrium phase diagram elucidating the influence of temperature and strain rate variations on solute-dislocation interaction.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"7194"},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced expression of dystrophin, IGF-1, CD44 and MYH3 in plasma and skeletal muscles including diaphragm of mdx mice after oral administration of Neu REFIX beta 1,3-1,6 glucan.","authors":"Senthilkumar Preethy, Shuji Sakamoto, Takuma Higuchi, Koji Ichiyama, Naoki Yamamoto, Nobunao Ikewaki, Masaru Iwasaki, Vidyasagar Devaprasad Dedeepiya, Subramaniam Srinivasan, Kadalraja Raghavan, Mathaiyan Rajmohan, Rajappa Senthilkumar, Samuel Jk Abraham","doi":"10.1038/s41598-025-92258-4","DOIUrl":"https://doi.org/10.1038/s41598-025-92258-4","url":null,"abstract":"<p><p>Duchenne muscular dystrophy (DMD) is a rare genetic disease, causing muscle degeneration due to lack of dystrophin with inadequate muscle regeneration culminating in muscle dysfunction. The N-163 strain of Aureobasidium Pullulans produced Beta-1,3 - 1,6-glucan (Neu REFIX) reported to be safe with anti-inflammatory and anti-fibrotic efficacy earlier, herein we evaluated its effects on muscle regeneration in mdx mice. Forty-five mice in three groups (n = 15 each): Group 1 (normal), Group 2 (mdx control), and Group 3 (mdx fed Neu REFIX) were evaluated for 45 days. IGF-1, Dystrophin, CD44 and MYH3 in diaphragm, plasma and skeletal muscle were evaluated by ELISA and immunohistochemistry. Mean IGF-1 expression was 20.32% and 16.27% higher in plasma (p = 0.03) and diaphragm respectively in Neu-REFIX group. Mean dystrophin was higher in Neu-REFIX group by 70.3% and 4.7% in diaphragm and plasma respectively than control. H-score intensity of CD44 + was > 2.0 with an MYH3-positivity 20% higher in Neu-REFIX than control. Oral administration of Neu REFIX was safe. Significantly enhanced plasma IGF-1 beside increased Dystrophin, MYH3 and CD44, proving a restoration of muscle regeneration and differentiation, especially in diaphragm, makes us recommend it as a disease modifying adjuvant in both early and advanced stages of DMD.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"7232"},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing the neural wave structures in the field of neuroscience.","authors":"Usman Younas, Jan Muhammad, D K Almutairi, Aziz Khan, Thabet Abdeljawad","doi":"10.1038/s41598-025-91397-y","DOIUrl":"https://doi.org/10.1038/s41598-025-91397-y","url":null,"abstract":"<p><p>Soliton theory research has a substantial impact on the application of nonlinear sciences in different fields. This has led to a significant increase in the focus of researchers on the study of solitary waves in recent years. This study explores the diverse dynamic behaviors exhibited by soliton solutions within the framework of the soliton neuron model. In neuroscience, this model is regarded as an important tool for comprehending the initiation and propagation of action potentials along axons through the application of a thermodynamic theory of nerve pulse transmission. The model proposed herein suggests that signals that propagate through the cell membrane can be represented as solitons, or solitary sound pulses. In order to analyze these soliton solutions, the nonlinear differential equation is transformed into the corresponding ordinary differential equation using a wave transformation. The wave profiles of the soliton neuron model are derived by using the Kumar-Malik method, multivariate generalized exponential rational integral function method, and Riccati modified extended simple equation method. These methods are implemented to extract a diverse array of soliton solutions, such as mixed, dark, bright-dark, singular, bright, complex, and combined solitons. This examination is focused on specific nonlinear phenomena of the proposed model. Numerous graphs are incorporated to clarify the behavior of solutions across a diverse range of parameter values. By validating the effectiveness of current methodologies and elucidating the nonlinear dynamic characteristics of a system, this research makes a substantial contribution to the domains of nonlinear science and higher-dimensional nonlinear wave fields. The insights presented in this paper can be implemented to address analytical challenges in various nonlinear systems in biological, technological, and physical systems to facilitate the comparison of computational and experimental data.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"7181"},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An accurate DNA and RNA based targeted sequencing assay for clinical detection of gene fusions in solid tumors.","authors":"Gang Ji, Qianlan Yao, Min Ren, Qianming Bai, Xiaoli Zhu, Xiaoyan Zhou","doi":"10.1038/s41598-025-91640-6","DOIUrl":"https://doi.org/10.1038/s41598-025-91640-6","url":null,"abstract":"<p><p>Gene fusions are one of the most important molecular biomarkers for tumor diagnosis, classification and targeted therapy. How to accurately detect them is a key issue in clinical work. In this study, a custom-designed integration of DNA and RNA-based next generation sequencing (NGS) assay including 16 targeted therapy related genes was developed and validated to identify gene fusions in solid tumors. This assay accurately identified all 10 different types of fusion in 8 commercial fusion spiked-in reference standards and 29 fusions including 16 different fusion forms in 60 clinical solid tumor samples previously identified by clinical testing methods. In addition, a TPM3::NTRK1 fusion was additionally identified and validated by Sanger sequencing, which showed a false-negative result for the previous result. Mutational abundance limit of detection for the assay was assessed with a series of dilution experiments. These fusions can be stably detected when the mutational abundance is down to 5% for DNA and 250-400 copies/100 ng for RNA. The intra-assay and inter-assay reproducibility was observed in three samples and three replicates. This integration of DNA and RNA-based NGS assay shows excellent performance on formalin-fixed, paraffin-embedded samples, results at different levels can complement each other, thereby facilitating precise diagnosis and treatment.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"7223"},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author Correction: Correlation between intestinal CRE colonization and consequent systemic infection in hospitalized patients in China.","authors":"Yuanyuan Xiao, Juping Duan, Caixia Tan, Ju Zou, Siyao Chen, Ting Liu, Lina Zhang, Xin Chen, Yajing Xu, Yuanyuan Li, Anhua Wu, Chunhui Li","doi":"10.1038/s41598-025-90543-w","DOIUrl":"https://doi.org/10.1038/s41598-025-90543-w","url":null,"abstract":"","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"7165"},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting MBTI personality of YouTube users.","authors":"Luisa Stracqualursi, Patrizia Agati","doi":"10.1038/s41598-025-85183-z","DOIUrl":"https://doi.org/10.1038/s41598-025-85183-z","url":null,"abstract":"<p><p>For several decades, the standard method of measuring personality has been through self-report questionnaires. In this work, we apply a model based on the analysis of the words used by people. Using Machine Learning (ML) and Natural Language Processing (NLP) techniques, we created a classifier that categorizes people into their Myers-Briggs Type Indicator (MBTI) personality type based on text samples extracted from their social media comments. Then, we applied this classifier to determine the MBTI personality types of YouTube users who comment on videos related to conspiracy theories, spirituality, and travel. The results highlight that a sufficient estimate can be made with only 100 words per user, as long as all 100 words are related to the same topic. The most common MBTI type among YouTubers who comment on these types of videos is INFP, followed by INTP, INFJ, and INTJ, indicating a predominance of Introverted and Intuitive individuals. While the predominant personality type in YouTuber comments on videos related to conspiracy theories is INTP, in spiritual and travel topics, the most common personality type is INFP.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"7221"},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality characteristics and consumer perception of non-alcoholic beers in the context of responsible alcohol consumption.","authors":"Anna Gliszczyńska-Świgło, Inga Klimczak, Dorota Klensporf-Pawlik, Iga Rybicka","doi":"10.1038/s41598-025-89833-0","DOIUrl":"https://doi.org/10.1038/s41598-025-89833-0","url":null,"abstract":"<p><p>Consumers are becoming more aware of the benefits of reducing alcohol consumption, which has increased the popularity of non-alcoholic beer (NAB). However, the brewing industry still faces many difficulties in producing NAB with similar physicochemical and sensory characteristics to regular beers. To mitigate these challenges, a multidisciplinary study was conducted to: (1) determine selected quality characteristics of various NABs, (2) compare them with literature data for regular beers, and (3) find out how Polish consumers, especially young people, perceive NAB and what motivates them in their purchasing decisions. Physicochemical quality characteristics of NABs, namely pH (4.3-4.7), bitterness (IBU = 12-77), colour (EBC = 6.4-19.6), total soluble solids (2.8-8.3 °Bx), and vitamin B₂ content (0.11-0.30 mg/L) were similar to alcoholic beers. Total phenolics (150-321 mg/L), Ca (13-45 mg/L), Mg (40-94 mg/L), K (192-490 mg/L), Na (0.5-57 mg/L), and P (158-467 mg/L) contents in NABs were slightly lower than in alcoholic beers, but this assortment is a healthier source of these nutrients due to the lack of alcohol-related harm. According to the consumer study, taste and availability in shops were found to be crucial factors influencing NAB purchasing decisions.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"7145"},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between renal oxidative stress levels and disease severity in patients with chronic kidney disease assessed by [Cu-64]ATSM PET/MRI.","authors":"Ya'nan Huang, Yudai Nishikawa, Tetsuya Mori, Munenobu Nogami, Akira Makino, Yasushi Kiyono, Tadashi Toyama, Hidehiko Okazawa","doi":"10.1038/s41598-024-85027-2","DOIUrl":"https://doi.org/10.1038/s41598-024-85027-2","url":null,"abstract":"<p><p>The purpose of the study was to investigate renal oxidative stress (OS) and its relationship with disease severity in patients with chronic kidney disease (CKD) using positron emission tomography coupled with magnetic resonance imaging (PET/MRI), employing ⁶⁴Cu-diacetyl-bis(N⁴-methylthiosemicarbazonate) (⁶⁴Cu-ATSM) as the PET tracer for OS imaging. Thirty patients with CKD (66.4 ± 8.2 y.o.) and seven healthy controls (HC) subjects (58.3 ± 3.8 y.o.) underwent ⁶⁴Cu-ATSM PET/MRI. Participants were categorized into three groups based on their estimated glomerular filtration rate (eGFR): HC, mild CKD (stages 2-3a), and advanced CKD (stages 3b-5). All subjects underwent 30-min dynamic PET/MRI starting with the injection of ⁶⁴Cu-ATSM to evaluate renal blood flow (RBF) and OS levels. RBF (mL/min/100 g) images were calculated from the first 3 min PET data, and standardized uptake value (SUV) images were obtained from delayed frames of 15-30 min after injection. The ⁶⁴Cu-ATSM SUV images were corrected to RBF-adjusted SUV using individual RBF images to estimate the OS levels of individual kidneys using the following equation: adjusted OS index (aOSi) = (SUV/RBF)x100. Significant correlation was observed between eGFR and RBF (r = 0.81, P < 0.001). RBF in patients with advanced CKD is significantly lower than that in HC (P < 0.001) and patients with mild CKD (P = 0.004). ⁶⁴Cu-ATSM SUV did not differ significantly among the three groups (P = 0.171). ⁶⁴Cu-ATSM SUVs did not correlate with creatinine in the HC subjects or in the patients with CKD. However, these values did correlate with eGFR (r = 0.33, P = 0.049) in all subjects, whereas the CKD patients showed no significant correlation. Following RBF correction, the aOSi demonstrated significant correlations with creatinine (r = 0.75, P < 0.001), eGFR (r= -0.65, P < 0.001), and CKD stages (r = 0.57, P < 0.001) in all subjects. This preliminary study has revealed that ⁶⁴Cu-ATSM PET may provide a estimate of renal OS reasonably in CKD patients noninvasively. Increased aOSi values were correlated with the CKD stages and creatinine levels, suggesting that OS increases with the severity of renal dysfunction.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"7227"},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}