Scientific Reports最新文献

{"title":"Impacts of land use and water level fluctuation on soil aggregates and nitrogen and carbon in newly formed reservoir buffer strips.","authors":"Fei Gu, Yong Qin, Tiezhu Yang, Xiumei Li, Tengfei Yan","doi":"10.1038/s41598-025-90224-8","DOIUrl":"https://doi.org/10.1038/s41598-025-90224-8","url":null,"abstract":"<p><p>Organic matter distribution patterns and soil aggregate stability (SAS) are critical factors for comprehending the environmental evolution of reservoirs and their surroundings. However, it remains unclear how SAS and aggregate-related N and C respond to external environmental factors (e.g., land use, water level fluctuation) in the newly formed reservoirs' buffer strips. In this work, we examined the impacts of the distance from the watercourse and different land use types on SAS and the patterns of organic matter distribution before and after seasonal water level fluctuations (in April and June 2021) in the Chushandian Reservoir (CR) buffer strips. The research discovered that the mean weight diameter (MWD) and geometric mean diameter (GMD) of soil aggregates varied across land uses, ranking as: woodland > grassland > abandoned cropland. Moreover, both MWD and GMD increased as the distance from the watercourse grew. Based on variation partitioning analysis and partial least squares path modelling results, distance from the watercourse showed less effect on nutrient content and SAS than land uses, and aggregate fractions played a mediating role in this process. Furthermore, the relative contribution of coarse aggregate fractions (macroaggregates, > 0.25 mm) to soil N and C levels was greater than that of fine aggregate fractions (microaggregates, < 0.25 mm). The research findings illustrate that various protection measures should be considered for different land use strategies to enhance SAS and promote the accumulation of N and C associated with aggregates in the CR buffer strips. In addition, these findings can provide valuable references for ecological restoration efforts in regions with comparable situations.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11181"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insecticidal effects of nano-encapsulated lemongrass essential oil on the population parameters of Spodoptera frugiperda using two-sex life table.","authors":"Samar Sayed Ibrahim, Magdy Youssef El-Kholy, Shehata El-Sayed Mohamed Shalaby","doi":"10.1038/s41598-025-93779-8","DOIUrl":"https://doi.org/10.1038/s41598-025-93779-8","url":null,"abstract":"<p><p>Essential oils derived from plants have demonstrated their efficacy in controlling insects; however, their poor physical characteristics restrict their widespread application. In this study, nano-encapsulation employing emulsification followed by ultrasonication technique has improved the insecticidal efficacy of lemongrass oil (LGO) against Spodoptera frugiperda. Beta-cyclodextrin (β-CD) and gum Arabic (GA) were used as coating materials to protect the vital components of LGO. Different concentrations of LGO pure and nano-capsule formulations have been used to examine the biological development of S. frugiperda 1st larval instar using two sex life table. D-Limonene, E-Citral, and Z-Citral were identified as the main compounds in LGO by GC-MS analysis. The oil concentration affected the physical properties of nano-capsules. The LGO nano-capsules had particle sizes ranging from 279.63 to 471.93 nm and an encapsulation efficiency between 51.94 and 83.59%. Following 96 h, the LGO:β-CD/GA treatment of S. frugiperda had a lower LC₅₀ value (1.52%) than the LGO treatment (2.48%), indicating that the nano-capsules were more toxic. Nano-capsules had a significant adverse effect on the life table parameters of S. frugiperda. The results of this study indicate that encapsulating LGO with β-CD/GA inclusion enhanced the efficacy of lemongrass oil against S. frugiperda.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11138"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating single-cell RNA sequencing, WGCNA, and machine learning to identify key biomarkers in hepatocellular carcinoma.","authors":"Gang Wang, Jiaxing Zhang, Yirong Li, Yuyu Zhang, Weiwei Dong, Hengquan Wu, Jinglan Wang, Peiqing Liao, Ziqiang Yuan, Tao Liu, Wenting He","doi":"10.1038/s41598-025-95493-x","DOIUrl":"https://doi.org/10.1038/s41598-025-95493-x","url":null,"abstract":"<p><p>The microarray and single-cell RNA-sequencing (scRNA-seq) datasets of hepatocellular carcinoma (HCC) were downloaded from the Gene Expression Omnibus (GEO) database. Differential expression analysis and weighted gene co-expression network analysis (WGCNA) were used to identify HCC-related biomarkers. Based on an analysis of scRNA-seq data, several marker genes expressed on tumor cells have been identified. Three machine-learning algorithms were used to identify shared diagnostic genes. Furthermore, logistic regression analysis was conducted to re-evaluate and identify essential biomarkers, which were then employed to develop a diagnostic prediction model. Additionally, AutoDockTools were used for molecular docking to investigate the association between the most sensitive drug and the core proteins. 44 genes were obtained by intersecting the WGCNA results, marker genes from scRNA-seq data, and up-regulated DEGs. Three machine-learning algorithms refined CDKN3, PPIA, PRC1, GMNN, and CENPW as hub biomarkers. GMNN and PRC1 were further selected by logistic regression analysis to build a nomogram. The molecular docking results showed that the drug NPK76-II-72-1 had a good binding ability with the GMNN and PRC1 proteins. The results highlighted CDKN3, PPIA, PRC1, GMNN, and CENPW as potential detection biomarkers for HCC patients. Our research offers novel insights into the diagnosis and treatment of HCC.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11157"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cable vibration and girder displacement measurement via morphology recognition and feature matching methods in bridge structure.","authors":"Wenkang Du, Hao Wang, Dong Lei, Zongqing Hang, Can Cui, Jintao He","doi":"10.1038/s41598-025-93625-x","DOIUrl":"https://doi.org/10.1038/s41598-025-93625-x","url":null,"abstract":"<p><p>The operational safety of bridges plays an important role in national or regional economic development. Due to complex variation loads, many engineering structures experience degradation and damage with increased operating time. Therefore, it is necessary to provide effective support for the maintenance of engineering structures. Take the traffic structure as an example, a large number of bridges in cities still rely on periodic inspections. Due to limited cost, existing periodic maintenance focused on observing surface damage and paying less attention to dynamic response in operation. Therefore, a measurement system based on a smartphone for full-field deformation of the bridge was introduced. Firstly, many vision-based methods for multiple bridge members measurement were proposed and the accuracy of methods was verified through laboratory and field experiments. With the combination of template matching and feature detection methods, the displacements of the deck were measured with an error of less than 0.3%. The dynamic response can be obtained with an error of less than 1% with the mapping model. Moreover, the stability and accuracy of the proposed vision-based methods under various illumination conditions were further explored. Finally, the recommended system has significant advantages of automated processing, good portability and low cost, providing a new solution for the maintenance of the bridges in the city.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11131"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of sarcopenia in patients with unresectable colorectal liver metastases after drug-eluting beads transcatheter arterial chemoembolization: a single center retrospective study.","authors":"Fuquan Wang, Bingxin Gong, Lei Chen, Yanyan Cao, Licheng Zhu, Bin Chai, Jihua Wang, Guofeng Zhou, Chuansheng Zheng","doi":"10.1038/s41598-025-95782-5","DOIUrl":"https://doi.org/10.1038/s41598-025-95782-5","url":null,"abstract":"<p><p>Sarcopenia is a prevalent condition in tumor patients and can potentially impact the prognosis of tumor treatment. This retrospective study aimed to evaluate the correlations between sarcopenia and the prognosis of patients with unresectable colorectal liver metastases (CRLM) received drug-eluting beads transcatheter arterial chemoembolization (DEB-TACE) therapy. From December 2018 to December 2023, unresectable CRLM patients who had already received second-line therapy from the Wuhan Union Hospital were involved in our study. Skeletal muscle mass was evaluated on CT at the L3 vertebra, and the optimal cut-off point for skeletal muscle index classification was determined using x-tile software. Overall survival (OS) and progression-free survival (PFS) were estimated using Kaplan-Meier analysis and Cox regression analysis. Seventy-one patients were included in the study, 34 with sarcopenia (sarcopenia group) and 37 without sarcopenia (non-sarcopenia group), respectively. The median PFS and OS was elevated in the non-sarcopenia group compared with the sarcopenia group (6.1 months versus 4.3 months, p = 0.012; 14.8 months versus 10.2 months, p < 0.001). The multivariate Cox regression analysis revealed that sarcopenia, extrahepatic metastases, and neutrophil-to-lymphocyte ratio (NLR) ≥ 5 were identified as independent risk factors for both PFS and OS. The advantages of non-sarcopenia in terms of OS were consistent across all subgroups examined. Additionally, the sarcopenia group exhibited a higher incidence of vomiting/nausea, fatigue, and abdominal pain following the DEB-TACE operation compared to the non-sarcopenia group. Sarcopenia demonstrated a substantial predictive value for both PFS and OS in unresectable CRLM patients who underwent DEB-TACE treatments. Besides, NLR > 5 and extrahepatic metastases were independent risk factors linked to a poorer prognosis. Furthermore, patients with sarcopenia may face an increased likelihood of experiencing adverse events following DEB-TACE treatments.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11097"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on 3D LiDAR outdoor SLAM algorithm based on LiDAR/IMU tight coupling.","authors":"Darong Zhu, Qi Wang, Fangbin Wang, Xue Gong","doi":"10.1038/s41598-025-95730-3","DOIUrl":"https://doi.org/10.1038/s41598-025-95730-3","url":null,"abstract":"<p><p>Aiming at the problems of easy loss of GPS positioning signals in outdoor environments and inaccurate map construction and position drift of traditional SLAM algorithms in outdoor scenes, this paper proposes a 3D LiDAR and inertial guidance tightly coupled SLAM algorithm. Firstly, inertial measurement unit (IMU) forward propagation is used to predict the current position, then backward propagation is used to compensate the motion distortion in the LiDAR data, and the point cloud alignment residuals are constructed based on the GICP algorithm, and then the iterative error state Kalman filter (IESKF) algorithm is utilized to complete the fusion of the point cloud residuals and the a priori position obtained from the forward propagation of the IMU to complete the state updating, and then the front-end fusion odometer is constructed. Next, a sparse voxel near-neighbor structure, iVox-based method, is employed to select key frames and construct local maps, leveraging spatial information during frame-map matching. This approach reduces the computational time required for point cloud alignment. Finally, the proposed algorithm is validated in real-world scenarios and on the outdoor open-source dataset KITTI. It is compared against mainstream algorithms, including FAST-LIO2 and LIO-SAM. The results demonstrate that the proposed approach achieves lower cumulative error, higher localization accuracy, and improved visualization with greater robustness in outdoor environments.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11175"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and validation of optimal real-time PCR reference genes for Abelmoschus Manihot.","authors":"Qixuan Wu, Meixin Deng, Xiaolan Zhao, Jianmei Long, Jianxia Zhang","doi":"10.1038/s41598-025-96110-7","DOIUrl":"https://doi.org/10.1038/s41598-025-96110-7","url":null,"abstract":"<p><p>Abelmoschus Manihot is an important medicinal and edible plant known for its functional secondary metabolites. However, little is known about the key genes involved in production of secondary metabolites in A. manihot. This is largely due to the lack of effective gene expression detection systems for A. manihot, making the screening of real-time PCR reference genes a prerequisite. In this study, 11 candidate reference genes were screened and cloned from A. manihot, and their expression stability was evaluated in different tissues under different flowering stages using four algorithms: geNorm, NormFinder, BestKeeper, and RefFinder. The expression stability of eIF and PP2A1 was the highest, while that of tubulin alpha (TUA) was the lowest. The combined use of the two most stable reference genes, eIF and PP2A1, met the experimental requirements for normalizing gene expression in A. manihot. Furthermore, the gene expression of transcription factors bHLH147 and bHLH148 was further validated by data normalization. This study identified potential reference genes in different A. manihot tissues, paving the way for functional gene analysis and dissecting metabolite regulation mechanisms in A. manihot.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11045"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Creep behavior of frozen soil and creep analytical model of frozen wall.","authors":"ZhengDong Chen, Bin Lin, HaiJie Hou","doi":"10.1038/s41598-025-91817-z","DOIUrl":"https://doi.org/10.1038/s41598-025-91817-z","url":null,"abstract":"<p><p>Taking the Gubei Mine as the research object, indoor uniaxial creep tests were carried out at temperatures of -5, -10, and -15℃ under loads of 0.3, 0.5, and 0.7σs to study the creep characteristics of deep soil, Based on the Burgers rheological model and the auxiliary formula of viscoelastic rheological formula, the freezing pressure under the combined action mechanism of frozen wall, outer shaft wall and polystyrene foam board was theoretically analyzed. The results show that the creep deformation of frozen clay under a certain constant stress loading changes with time. At three temperatures, the creep is attenuated creep under constant stress of 0.3 and 0.5σs; it exhibits non-attenuated creep at a constant stress level of 0.7σs, and the growth rate increases over a certain period of time, and finally it is destroyed by excessive deformation over time. The calculated value of the freezing pressure calculated by the frozen wall model is roughly the same as the development law of the measured value, and the calculated value of the theoretical value of the freezing pressure is slightly larger than the measured value, indicating that the calculated value can be an effective reference for the creep of the frozen wall and can provide a guarantee for the construction safety.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11193"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Signal size and resolution of scanning thermal microscopy in air and vacuum.","authors":"Jabez J McClelland, Evgheni Strelcov, Ami Chand","doi":"10.1038/s41598-025-95648-w","DOIUrl":"https://doi.org/10.1038/s41598-025-95648-w","url":null,"abstract":"<p><p>We present measurements comparing scanning thermal microscopy in air and vacuum. Signal levels are compared and resolution is probed by scanning over the edge of a nanofabricated Ag square embedded in [Formula: see text]. Signals measured in air were seen to be 2.5-40 times larger than in vacuum. Furthermore, the air signals were stable while the vacuum signals varied significantly. Edge widths measured in air were approximately 39% larger than those measured in vacuum. Our observations are consistent with the air measurements experiencing heat transfer from the surrounding sample through the air as well as the formation of a water-related meniscus at the tip-sample junction. These results contribute to the understanding of the complex heat exchange effects that can occur in scanning thermal microscopy when it is conducted in an ambient atmosphere.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11142"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and bioactivity evaluation of a novel autotaxin inhibitor with anti-hepatic fibrosis effects.","authors":"Yuzheng Liu, Dan Luo, Hongwen Leng, Chaolun Gu, Qingrong Ding, Lin Zhao, Xinxin Chao, Hanxia Wang, Yueteng He, Silu Zhu, Guanhua Ai, Weijie Peng","doi":"10.1038/s41598-025-95464-2","DOIUrl":"https://doi.org/10.1038/s41598-025-95464-2","url":null,"abstract":"<p><p>Autotaxin (ATX) is considered as a serum marker of hepatic fibrosis, which is positively correlated with the degree of hepatic fibrosis. However, there are no clinical studies on anti-hepatic fibrosis drugs targeting ATX. This study attempts to find novel ATX small molecule inhibitors based on virtual screening methods including two-dimensional similarity search, pharmacophore screening, molecular docking, drug-like properties and ADMET filtration, combined with biological evaluation. An ATX inhibitor (IC50 = 43.05 µmol/L) is discovered by our screening strategy. In vivo result show that the novel ATX inhibitor represents excellent anti-hepatic fibrosis effects in mice. This screening strategy had potential significance for the discovery of ATX inhibitors in the future.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"11092"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}