Spatiotemporal dynamics of benzylisoquinoline alkaloid gene expression and co-expression networks during Papaver Somniferum developmental stages.

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-07-28 DOI:10.1038/s41598-025-11942-7

Zishi Wang, Quanzheng Yun, Jinyuan Hu, Zhen Wei, Deri Feng, Ning Li, He Xu, Lihong Fu, Zhenlong Wang, Shujin Li, Fangru Liu, Yan Wang, Bin Cong, Baishi Wang

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引用次数: 0

Abstract

Benzylisoquinoline alkaloids (BIAs) are essential secondary metabolites produced by Papaver somniferum, widely recognized for their pharmaceutical importance. This study employs transcriptome sequencing and weighted gene co-expression network analysis (WGCNA) to investigate the spatiotemporal expression patterns and regulatory networks of BIA-related genes across developmental stages and organs. A total of 23 co-expression modules were identified, revealing stage- and organ-specific dynamics in BIA biosynthesis. Key genes such as TYDC, PPO, and GsSRK demonstrated distinct regulatory roles during flowering and fruit maturation. Functional enrichment analysis uncovered critical pathways and transcription factors involved in alkaloid production. These findings enhance our understanding of the molecular regulation of BIAs and provide valuable insights for improving alkaloid yield through metabolic engineering and molecular breeding strategies.

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木瓜发育阶段苯基异喹啉生物碱基因表达及共表达网络的时空动态

苄基异喹啉生物碱（BIAs）是罂粟产生的重要次生代谢产物，具有重要的药用价值。本研究采用转录组测序和加权基因共表达网络分析（WGCNA）研究bia相关基因在发育阶段和器官间的时空表达模式和调控网络。共鉴定出23个共表达模块，揭示了BIA生物合成的阶段和器官特异性动力学。TYDC、PPO和GsSRK等关键基因在开花和果实成熟过程中表现出不同的调控作用。功能富集分析揭示了生物碱生产的关键途径和转录因子。这些发现增强了我们对生物碱的分子调控的认识，并为通过代谢工程和分子育种策略提高生物碱产量提供了有价值的见解。

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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