Sheng wu gong cheng xue bao = Chinese journal of biotechnology最新文献

{"title":"[Partial knockout of <i>NtPDK1a</i>/<i>1b</i>/<i>1c</i>/<i>1d</i> enhances the disease resistance of <i>Nicotiana tabacum</i>].","authors":"Qianwei Ren, Hujiao Lan, Tianyao Liu, Huanting Zhao, Yating Zhao, Rui Zhang, Jianzhong Liu","doi":"10.13345/j.cjb.240231","DOIUrl":"https://doi.org/10.13345/j.cjb.240231","url":null,"abstract":"<p><p>The protein kinase A/protein kinase G/protein kinase C-family (AGC kinase family) of eukaryotes is involved in regulating numerous biological processes. The 3-phosphoinositide- dependent protein kinase 1 (PDK1), is a conserved serine/threonine kinase in eukaryotes. To understand the roles of <i>PDK1</i> homologous genes in cell death and immunity in tetraploid <i>Nicotiana tabacum</i>, the previuosly generated transgenic CRISPR/Cas9 lines, in which 5-7 alleles of the 4 homologous <i>PDK1</i> genes (<i>NtPDK1a</i>/<i>1b</i>/<i>1c</i>/<i>1d</i> homologs) simultaneously knocked out, were used in this study. Our results showed that the hypersensitive response (HR) triggered by transient overexpression of active Pto (Pto^Y207D) or soybean <i>Gm</i>MEKK1 was significantly delayed, whereas the resistance to <i>Pseudomonas syrangae</i> pv. <i>tomato</i> DC3000 (<i>Pst</i> DC3000) and tobacco mosaic virus (TMV) was significantly elevated in these partial knockout lines. The elevated resistance to <i>Pst</i> DC3000 and TMV was correlated with the elevated activation of <i>Nt</i>MPK6, <i>Nt</i>MPK3, and <i>Nt</i>MPK4. Taken together, our results indicated that <i>NtPDK</i>1s play a positive role in cell death but a positive role in disease resistance, likely through negative regulation of the MAPK signaling cascade.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"670-679"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Regulatory roles of JAZ in the growth and development of horticultural plants].","authors":"Xinxin Zhang, Tao Tao, Hangchun Li, Zhi Qiao, Qinglin Tang, Dayong Wei, Yang Yang, Zhimin Wang","doi":"10.13345/j.cjb.240547","DOIUrl":"https://doi.org/10.13345/j.cjb.240547","url":null,"abstract":"<p><p>Jasmonic acid (JA) is a common plant hormone with regulatory effects on plant growth and development. The jasmonate ZIM-domain (JAZ) proteins (JAZs), as key regulators in the JA signaling pathway, are involved in multiple biological processes such as anthocyanin accumulation, flowering time modulation, and secondary metabolite synthesis in plants. JAZs are essential components of many regulatory signaling networks. The <i>JAZ</i> genes, members of the plant-specific TIFY family, have been identified in the genomes of a variety of horticultural plants. Here, we summarized the research progress in the roles of JAZs in horticultural plants, aiming to give insights into the further study of the biological functions and regulatory networks of <i>JAZ</i> genes in plants.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"530-545"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Cloning and expression analysis of the laccase gene <i>RcLAC15</i> from <i>Rosa chinensis</i>].","authors":"Qi Li, Yifang Peng, Qijing Dong, Qian Yang, Xiaoyu Liu, Yu Han","doi":"10.13345/j.cjb.240141","DOIUrl":"https://doi.org/10.13345/j.cjb.240141","url":null,"abstract":"<p><p>Laccases (LACs), belonging to the multicopper oxidase family, are closely associated with various biological functions including lignin synthesis and responses to biotic and abiotic stresses in plants. However, few studies have reported the laccase genes in China rose (<i>Rosa chinensis</i>). Prickles cause difficulties to the management and harvest of <i>R</i>. <i>chinensis</i> and have become a trait concerned in the breeding. To investigate the expression patterns of laccase genes in roses, we cloned a laccase gene from an ancient variety <i>R</i>. <i>chinensis</i> 'Old Blush' and named it <i>RcLAC15</i>. The expression level of <i>RcLAC15</i> in prickles was significantly higher than those in roots, stems, and leaves. Fifty-eight laccase genes were identified in the genome of <i>R</i>. <i>chinensis</i>, and bioinformatics analysis revealed that <i>RcLAC15</i> was a homolog of <i>AtLAC15</i>, predicting that RcLAC15 was a stable hydrophilic protein without transmembrane structures. The recombinant expression vector pBI121-proRcLAC15:: <i>GUS</i> was introduced into <i>Arabidopsis</i>, and GUS staining results showed that the <i>RcLAC15</i> promoter specifically drove <i>GUS</i> gene expression at the edges of <i>Arabidopsis</i> leaves. In summary, <i>RcLAC15</i> is a gene specifically expressed in the prickles of <i>R</i>. <i>chinensis</i>. This discovery provides a reference for exploring the biological functions of laccase genes in the prickles of <i>R</i>. <i>chinensis</i>.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"845-856"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Promotion of <i>Stenotrophomonas</i> sp. on the photosynthetic growth of microalgae exposed to high concentrations of formate].","authors":"Mengmeng Xing, Weijie Zheng, Wangyin Wang, Xupeng Cao, Can Li","doi":"10.13345/j.cjb.240242","DOIUrl":"https://doi.org/10.13345/j.cjb.240242","url":null,"abstract":"<p><p>Formate is an important solar fuel, with large application potential in bioconversion. Especially, the win-win collaboration is achieved when formate is applied to the cultivation of microalgae, which combines the advantages from both artificial and natural photosynthesis. However, the inhibition of formate on the photosynthetic electron transport hinders the application of formate at high concentrations. The engineering or directed evolution of the regulation pathway is a case-by-case and time-consuming strategy. Here, we developed a new strategy by introducing a <i>Stenotrophomonas</i> sp. strain which was isolated and identified from the long-term self-evolution process of <i>Chlamydomonas reinhardtii</i> for adapting to high concentrations of formate. The co-culture with the strain or the fermentation broth relieved the inhibition of formate (50 mmol/L) on <i>C</i>. <i>reinhardtii</i> and promoted the growth of the microalga. Especially, the protein content increased significantly to nearly 50% of the dried weight. In addition, the co-culture also benefited the growth of both <i>Chlorella pyrenoidesa</i> and <i>Synechocystis</i> sp. PCC 6803 exposed to formate, which indicated broader applicability of this strategy. This strategy provides the opportunity to overcome the bottleneck in the formate-mediated artificial-natural hybrid photosynthesis and to aid the development of technologies for solar energy-driven production of bulk biomass, including proteins, by carbon dioxide reduction.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 1","pages":"230-241"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143040855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[<i>Pseudomonas monteilii</i> ZMU-T06 produces 2-substituted quinolines by oxidative dehydroaromatization].","authors":"Min Yang, Lan Zou, Huimin Ran, Lei Qin","doi":"10.13345/j.cjb.240359","DOIUrl":"https://doi.org/10.13345/j.cjb.240359","url":null,"abstract":"<p><p>2-substituted quinolines are the building blocks for the synthesis of natural products and pharmaceuticals. In comparison with classical methods, dehydroaromatization of 2-substituted-1,2,3,4-tetrahydroquinolines has emerged in recent years as an efficient and straightforward method to synthesize quinolines due to its high atom economy and sustainability. However, existing chemical methods need transition metal catalysts and harsh reaction conditions. Biocatalysis with high efficiency, high selectivity, and mild reaction conditions has become an important method of organic synthesis. We mined a strain <i>Pseudomonas monteilii</i> ZMU-T06 capable of producing monoamine oxidase for the dehydroaromatization of 2-substituted-1,2,3,4-tetrahydroquinolines to synthesize 2-substituted quinolines (8 substrates, yields of 45.7%-48.4%) and then hypothesized the catalytic mechanism, providing a new method for green synthesis of 2-substituted quinolines.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 1","pages":"288-295"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Establishment and application of a genetic operating system in <i>Wickerhamomyces ciferrii</i> for the synthesis of tetraacetyl phytosphingosine].","authors":"Liu Liu, Zheng'an Yin, Li Pan","doi":"10.13345/j.cjb.240385","DOIUrl":"https://doi.org/10.13345/j.cjb.240385","url":null,"abstract":"<p><p><i>Wickerhamomyces ciferrii</i> (<i>W</i>.<i>c</i>), an unconventional heterothallic yeast species, is renowned for its high production of tetraacetyl phytosphingosine (TAPS). Due to its excellent performance in TAPS production, this study aimed to construct a genetic operating system of <i>W</i>.<i>c</i> to enhance the production of TAPS and to screen high-yielding strains by mutagenesis and genetic engineering, thus laying the foundation for further development of industrial production of sphingolipid metabolites. In this study, we selected two autonomous replication elements (CEN, 2μ) and mined 11 endogenous promoter elements to establish a genetic operating system in <i>W</i>. <i>ciferrii</i>. The overexpression of <i>Syr2</i> and <i>Lcb2</i> in the sphingolipid metabolism pathway significantly increased the production of TAPS. Meanwhile, we established a method for the identification of haploid mating types of <i>W</i>. <i>ciferrii</i> by combining RT-PCR and flow cytometry. Five strains of <i>W</i>. <i>ciferrii</i> with different mating types constructed from the standard diploid <i>W</i>. <i>ciferrii</i> ATCC 14091 were screened out. A-type haploid <i>W</i>.<i>c</i> 140 showcased the highest production of TAPS with a yield of 4.74 mg/g and a titer of 32.61 mg/L. Mutant strains <i>W</i>.<i>c</i> 140-A9 and <i>W</i>.<i>c</i> 140-A11 were induced by atmospheric pressure room temperature plasma mutagenesis. The recombinant strains <i>W</i>.<i>c</i> 140 OE<i>Lcb2</i> and <i>W</i>.<i>c</i> 140 OE<i>Syr2</i> with overexpression were constructed with the genetic operating system established in this study. The TAPS yields of the mutant strains increased by 61.39% and 67.09%, respectively, compared with that of starting strain <i>W</i>.<i>c</i> 140. The recombinant strains cultured in the LCBNB medium achieved yields of 10.60 mg/g and 12.14 mg/g, respectively, representing 2.24 and 2.56 times of that in strain <i>W</i>.<i>c</i> 140. Moreover, the yields of the two recombinant strains were significantly higher than that of the diploid strain ATCC 14091. The genetic operating system and the haploid strain <i>W</i>.<i>c</i> 140 established in this study provide a basis for the subsequent establishment of genetic engineering tools for <i>W</i>. <i>ciferrii</i>.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 1","pages":"397-415"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mining and characterization of new enzymes based on Phi29 DNA polymerase].","authors":"Mengyao Hao, Lingling Hu, Minghao Han, Congyu Li, Hong Chang, Jianmei Luo, Huifeng Jiang","doi":"10.13345/j.cjb.240222","DOIUrl":"https://doi.org/10.13345/j.cjb.240222","url":null,"abstract":"<p><p>In recent years, the bacteriophage Φ29 (Phi29) DNA polymerase has garnered increasing attention due to its high-fidelity amplification capacity at constant temperatures. To advance the industrial application of this type of isothermal polymerases, this study mined and characterized new enzymes from the microbial metagenome based on the known Phi29 DNA polymerase sequence. The results revealed that a new enzyme, Php29 DNA polymerase, was identified in the microbial metagenome with plants as the hosts. This enzyme exhibited higher strand displacement activity, with a 59.5% similarity to bacteriophage Φ29. Experimental validation demonstrated that the enzyme had 3'→5' exonuclease activity, and its amplification products can serve as substrates for further catalytic reactions. The discovery and validation of Php29 DNA polymerase gives insights into the future industrial application of isothermal polymerases.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 1","pages":"427-436"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Biomanufacturing driven by engineered organisms].","authors":"Huawei Zhu, Yin Li","doi":"10.13345/j.cjb.241014","DOIUrl":"https://doi.org/10.13345/j.cjb.241014","url":null,"abstract":"<p><p>This article reviews the review articles and research papers related to biomanufacturing driven by engineered organisms published in the Chinese Journal of Biotechnology from 2023 to 2024. The content covers 26 aspects, including chassis cells; gene (genome) editing; facilities, tools and methods; biosensors; protein design and engineering; peptides and proteins; screening, expression, characterization and modification of enzymes; biocatalysis; bioactive substances; plant natural products; microbial natural products; development of microbial resources and biopesticides; steroidal compounds; amino acids and their derivatives; vitamins and their derivatives; nucleosides; sugars, sugar alcohols, oligosaccharides, polysaccharides and glycolipids; organic acids and monomers of bio-based materials; biodegradation of polymeric materials and biodegradable materials; intestinal microorganisms, live bacterial drugs and synthetic microbiomes; microbial stress resistance engineering; biodegradation and conversion utilization of lignocellulose; C1 biotechnology; bioelectron transfer and biooxidation-reduction; biotechnological environmental protection; risks and regulation of biomanufacturing driven by engineered organisms, with hundreds of technologies and products commented. It is expected to provide a reference for readers to understand the latest progress in research, development and commercialization related to biomanufacturing driven by engineered organisms.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 1","pages":"1-78"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress in the design and application of whole-cell biosensors for antibiotics].","authors":"Yuke Luo, Yiling Zhu, Jianping Xu, Junfeng Liu, Jianhua Yin","doi":"10.13345/j.cjb.240400","DOIUrl":"https://doi.org/10.13345/j.cjb.240400","url":null,"abstract":"<p><p>Antibiotics are chemicals with bactericidal or bacteriostatic activity produced by microorganisms and artificially synthesized. Since the discovery of penicillin by Alexander Fleming in 1928, antibiotics have been widely used in clinical treatments as well as in the animal husbandry and aquaculture, leading to antibiotic residues in soil, water, food and other environments. At the same time, antibiotic resistance is increasingly serious, which necessitates the discovery of novel antibiotics. In recent years, with the development of synthetic biology, researchers have developed a variety of whole-cell biosensors that can respond to antibiotics. These whole-cell biosensors use microbial cells to convert antibiotic signals into readable signals, which can not only perform dynamic detection of antibiotics simply, quickly, sensitively and accurately but also effectively discover novel antibiotics. This review comprehensively summarizes the reported whole-cell biosensors for antibiotics, classifies them into two types (specific and general), and elaborates on the design principles and applications of the two types of antibiotic biosensors. This review will provide reference for the construction and application of other whole-cell biosensors for antibiotics.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 1","pages":"79-91"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Construction and application of an inducible transcriptional regulatory tool from <i>Medicago truncatula</i> in <i>Saccharomyces cerevisiae</i>].","authors":"Meilin Feng, Caifang Shi, Ying Wang, Chun Li","doi":"10.13345/j.cjb.240210","DOIUrl":"https://doi.org/10.13345/j.cjb.240210","url":null,"abstract":"<p><p>Transcriptional regulation based on transcription factors is an effective regulatory method widely used in microbial cell factories. Currently, few naturally transcriptional regulatory elements have been discovered from <i>Saccharomyces cerevisiae</i> and applied. Moreover, the discovered elements cannot meet the demand for specific metabolic regulation of exogenous compounds due to the high background expression or narrow dynamic ranges. There are abundant transcriptional regulatory elements in plants. However, the sequences and functions of most elements have not been fully characterized and optimized. Particularly, the applications of these elements in microbial cell factories are still in the infancy stage. In this study, natural regulatory elements from <i>Medicago truncatula</i> were selected, including the transcription factors MtTASR2 and MtTASR3, along with their associated promoter P_{<i>roHMGR1</i>}, for functional characterization and engineering modification. We constructed an inducible transcriptional regulation tool and applied it in the regulation of heterologous β-carotene synthesis in <i>S</i>. <i>cerevisiae</i>, which increased the β-carotene production by 7.31 folds compared with the original strain. This study demonstrates that plant-derived transcriptional regulatory elements can be used to regulate the expression of multiple genes in <i>S</i>. <i>cerevisiae</i>, providing new strategies and ideas for the specific regulation and application of these elements in microbial cell factories.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 1","pages":"363-375"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}