Sheng wu gong cheng xue bao = Chinese journal of biotechnology最新文献

{"title":"[Enzymatic intermolecular Stetter reaction catalyzed by benzaldehyde lyase].","authors":"Yuting Zhang, Yingjia Tong, Zhi Zhou","doi":"10.13345/j.cjb.260082","DOIUrl":"https://doi.org/10.13345/j.cjb.260082","url":null,"abstract":"<p><p>Biocatalysis, as a fundamental enabling technology of biomanufacturing, is indispensable for promoting the green and efficient transformation of industrial processes. The catalytic disorder of enzymes provides key support for the development of new chemical conversions that do not exist in nature. Therefore, we urgently need to expand the diverse new functions of enzymes. In this study, we report a case of an unnatural intermolecular Stetter reaction catalyzed by natural benzaldehyde lyase (<i>pf</i>BAL). Benzaldehyde lyase activates benzaldehyde to undergo an addition reaction with the imine substrate formed <i>in situ</i>, and thus an unnatural amino ketone is obtained. Through systematic screening and optimization of reaction conditions, the yield of the target product was enhanced, and a certain range of substrate applicability was demonstrated. This study demonstrates that the benzaldehyde lyase <i>pf</i>BAL exhibits catalytic activity in mediating the Stetter reaction between unnatural molecules, laying a theoretical foundation for the future expansion of diverse catalytic functions of this enzyme.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"4168-4178"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Protein engineering of PgsA combined with Bayesian optimization drives efficient poly-γ-glutamic acid synthesis by <i>Corynebacterium glutamicum</i>].","authors":"Wanjing Liu, Haozhe Zhou, Xiaogang Wang, Yuhang Chen, Jingjie Ma, Dejing Yin, Jianying Qian, Xiaomei Zhang, Guoqiang Xu, Jinsong Shi, Zhenghong Xu","doi":"10.13345/j.cjb.250895","DOIUrl":"https://doi.org/10.13345/j.cjb.250895","url":null,"abstract":"<p><p>Poly-γ-glutamic acid (γ-PGA) is a biopolymer polymerized from l-glutamic acid (l-Glu) and/or d-glutamic acid monomers, exhibiting broad application prospects in pharmaceuticals, cosmetics, and other fields. The low yields remain a key factor limiting large-scale production of γ-PGA in heterologous expression systems. To enhance the biosynthetic efficiency, this study employed <i>Corynebacterium glutamicum</i> as the chassis cell and utilized the γ-PGA synthase PgsBCA from <i>Bacillus licheniformis</i> to catalyze γ-PGA synthesis. First, a polycistron expression system was constructed, increasing the γ-PGA yield by 20.6% compared with the monocistron system. After site-directed and saturation mutagenesis of PgsA, the mutant K76C was identified, increasing the γ-PGA yield by 75.3%. On this basis, a Bayesian model was employed to optimize seven medium components including glucose and urea. The optimized medium achieved a γ-PGA yield of 14.52 g/L, which represented a 28.6% increase over that in the initial medium. Finally, the recombinant strain was scaled up in a 5 L fermenter. After 48 hours of fermentation, the γ-PGA yield reached 64.13 g/L, with a glucose conversion rate of 0.52 g/g and the l-Glu content of 100%. This study developed a high-yielding γ-PGA-producing engineered strain and established an efficient fermentation process, providing a novel strategy for the efficient production of γ-PGA.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"4106-4123"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Construction of tobacco BY-2 cell lines for heterologous synthesis of taxadiene and transcriptomic analysis].","authors":"Dairui Hou, Yingying Zhang, Hanqing Cong, Fei Qiao, Xingfen Miao","doi":"10.13345/j.cjb.260122","DOIUrl":"https://doi.org/10.13345/j.cjb.260122","url":null,"abstract":"<p><p>Paclitaxel is an active anticancer ingredient with extremely high medicinal value, whereas its natural plant resources are scarce. Taxadiene, as the first committed precursor in the biosynthesis of paclitaxel, is of great significance for the production of paclitaxel. This study aimed to establish an efficient heterologous synthesis system for taxadiene, so as to provide a theoretical basis and experimental support for its large-scale production. Using the wild-type tobacco BY-2 cell line and the plant expression vector <i>TS</i>-pk7FGW2, we constructed a tobacco BY-2 cell line heterologously expressing taxadiene synthase (TS) for synthesis of taxadiene. The accumulation of taxadiene in the BY-2 cell line heterologously expressing <i>TS</i> was the highest [0.55 μg/g fresh weight (FW)] under dark culture and reached 1.34 μg/g FW under light treatment, which was 1.4-fold higher than that under dark culture. Transcriptomic analysis was performed on 7-day-old wild-type and <i>TS</i>-expressing tobacco BY-2 cell line to identify differentially expressed genes and associated metabolic pathways. A total of 6 121 differentially expressed genes (DEGs) were identified in the <i>TS</i>-expressing line in comparison with the wild type. Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis showed that the MVA and MEP pathways in the terpenoid backbone biosynthesis pathway were affected in the tobacco BY-2 cells heterologously expressing <i>TS</i>. This study provides a theoretical basis for optimizing the efficiency of heterologous taxadiene synthesis in BY-2 cells and offers theoretical and data support for elucidating the molecular mechanism by which TS regulates the growth and development of BY-2 cells and the heterologous biosynthesis of taxadiene.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"4318-4331"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Machine learning-based optimization strategies for fermentation processes].","authors":"Faman Lu, Huayao Chen, Haiyan Zhou, Yuanshan Wang, Kun Niu, Zhiqiang Liu, Yuguo Zheng","doi":"10.13345/j.cjb.260303","DOIUrl":"https://doi.org/10.13345/j.cjb.260303","url":null,"abstract":"<p><p>Microbial fermentation technology is crucial in modern biomanufacturing processes, such as biopharmaceutical production. However, traditional fermentation process control faces bottlenecks including offline monitoring delay, reliance on empirical experience, and a lack of precise and real-time regulation. Machine learning (ML), with its powerful data extraction and predictive modeling capabilities, has emerged as a key tool for driving the transition from traditional fermentation processes to intelligent paradigms. This paper systematically reviewed the latest research progress on the closed-loop intelligent fermentation control systems, spanning from underlying data perception to high-level decision-making. First, the specific applications of ML in fermentation optimization were elaborated, with a focus on systematic modeling workflows in response to the inherent \"black-box\" nature and data-lag limitations of purely data-driven models. Second, by comparing the monitoring characteristics of various process analytical technologies, the critical supporting role of Raman spectroscopy in real-time fermentation monitoring was elucidated, and cutting-edge approaches employing semi-supervised learning and data augmentation strategies to address the scarcity of high-quality labeled samples were summarized. Furthermore, the ML-driven \"intelligent perception-feedback closed-loop\" control mechanism was thoroughly analyzed, and the transformation of fermentation control from traditional \"macroscopic feeding regulation\" to \"microscopic metabolic precise guidance\" was expounded. Finally, the future development trajectories for intelligent fermentation was envisioned from two perspectives, namely the construction of cross-genus universal predictive models and the application of digital twin technology. This review aims to provide valuable technical references for the intelligent upgrading of the biomanufacturing sector and the efficient development of fermentation processes.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"3846-3866"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Identification of key targets for ergothioneine biosynthesis based on random genomic transposition and high-throughput screening].","authors":"Weijie Tang, Yiming Zhao, Zun Tao, Bohan Cai, Yuan Zhang, Longtao Wang, Mengping Wu, Guangzhen Zhou, Jing Wu, Zhanzhi Liu","doi":"10.13345/j.cjb.260048","DOIUrl":"https://doi.org/10.13345/j.cjb.260048","url":null,"abstract":"<p><p>Ergothioneine (ERG), as a natural potent antioxidant, possesses excellent physicochemical properties and diverse physiological activities, thereby exhibiting broad application prospects. However, engineering of existing genetically modified strains is prone to encountering bottlenecks in yield improvement. To identify key regulatory genes involved in ERG biosynthesis and enhance its heterologous production efficiency in <i>Escherichia coli</i>, this study employed <i>E</i>. <i>coli</i> BL21(DE3) as the chassis and constructed a heterologous biosynthetic pathway comprising <i>Trichoderma reesei</i>-derived Tregt1 and Tregt2, yielding a foundational engineered strain P1 with a shake-flask fermentation titer of 650 mg/L. Subsequently, a genome-wide mutant strain library was constructed using a random integration system mediated by Himar1C9 transposase, and combined with a high-throughput screening method based on the mBBr fluorescent probe, four positive mutant strains exhibiting significant phenotypic differences were successfully obtained. Sequencing analysis revealed that the key mutated genes encode phosphoenolpyruvate synthase PpsA, vitamin B₁₂ transporter permease BtuC, DNA helicase RecG, and a bifunctional nicotinamidase/pyrazinamidase PncA. Shake-flask fermentation validation showed that overexpression of <i>recG</i> significantly increased the ERG titer to 797 mg/L, whereas overexpression of the other three genes reduced the yield. This study establishes a systemic strategy combining random transposon integration and high-throughput screening, thereby overcoming the limitations of conventional targeted engineering and laying a foundation for the construction of efficient ERG-producing engineered strains and the advancement of their industrial production.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"3986-3998"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Biosynthesis and application of curdlan].","authors":"Simei Zhang, Peisi Chen, Yuping Lin, Shuyao Yang, Liang Yin, Jiajia Li, Bin Liu, Xubin Fu, Jiansong Ju, Jinshan Li, Yanhe Ma","doi":"10.13345/j.cjb.260384","DOIUrl":"https://doi.org/10.13345/j.cjb.260384","url":null,"abstract":"<p><p>Curdlan is a water-insoluble <i>β</i>-1,3-glucan synthesized by microorganisms under nitrogen-limited conditions. It possesses unique physicochemical properties, such as gelation ability, freeze-thaw stability, water-holding capacity, and thickening capability, and is widely used in the food and pharmaceutical industries. In recent years, with the rapid development of the alternative protein (NeoProtein) sector, curdlan has demonstrated outstanding application potentials by significantly improving the fidelity and texture quality of meat analogues through matrix texture modification, animal fat mimicry, and flavor release modulation, and its efficient bio-manufacturing technology has thus become a research hotspot. This review analyzes and summarizes the structure and function, application areas, biosynthesis and regulatory mechanisms, and advances in biosynthesis technology of curdlan, and proposes future directions for trends for the development of curdlan biosynthesis technology, thereby providing theoretical support for future strain improvement, fermentation process optimization, and application exploration for curdlan biomanufacturing.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"3945-3969"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Microenvironmental regulation strategies and applications of yeast surface display systems].","authors":"Xin Luo, Weijie Cao, Jing Yang, Wenbin Jiang, Hu Liu, Chun Li","doi":"10.13345/j.cjb.260250","DOIUrl":"https://doi.org/10.13345/j.cjb.260250","url":null,"abstract":"<p><p>Yeast surface display (YSD) is an important technological platform integrating protein engineering, synthetic biology, and industrial biocatalysis. Its catalytic efficiency and stability highly depend on the dynamic synergy of the three-tier microenvironments: the cell wall, intracellular environment, and extracellular compartments. This review systematically elaborates on the core components of the YSD microenvironment, including the cell wall, anchor proteins, intracellular secretion pathways, and extracellular physicochemical properties, and clarifies their critical roles in the folding, activity, and anchoring efficiency of displayed proteins. In addition, this review summarizes targeted regulation strategies, including cell wall gene engineering, optimization of anchor proteins and linkers, enhancement of intracellular secretion pathways, adaptation of extracellular culture conditions, and spatial arrangement of multienzyme co-display. It reveals that synergistic optimization of the three-tier microenvironments can significantly improve display efficiency and catalytic performance. YSD technology based on microenvironment regulation has achieved substantial application progress in industrial production, biomedicine, and environmental remediation. However, current microenvironment regulation still faces challenges in terms of precision, compatibility, and stability. Future research should focus on developing corresponding regulation strategies to break through technical bottlenecks and promote the industrial application of YSD. Microenvironment regulation provides important theoretical and technical support for the performance optimization and industrial translation of YSD.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"3811-3828"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Advances in automated enrichment and directed domestication technologies and their applications in the development of environmental functional microorganisms].","authors":"Jiawei Jiang, Zhilin Xing, Tiantao Zhao, Ailing Chen","doi":"10.13345/j.cjb.260466","DOIUrl":"https://doi.org/10.13345/j.cjb.260466","url":null,"abstract":"<p><p>Environmental functional microorganisms play a key role in environmental engineering processes such as pollutant degradation, wastewater treatment, and resource utilization. Conventional enrichment and directed domestication technologies often have limitations such as long operational cycles and low throughput, being difficult to meet the demands for rapid screening, enhancement, and engineering application of functional microorganisms under complex environmental conditions. In recent years, advances in continuous cultivation, high-throughput cultivation, microfluidic chips, intelligent bioreactors, and automated adaptive laboratory evolution (ALE) have provided new approaches for the precise screening, targeted enhancement, and feedback-based process control of environmental functional microorganisms. This review systematically summarizes the basic principles and key platforms of automated enrichment and directed domestication technologies, as well as their application progress in the development of microorganisms for petroleum hydrocarbon and polycyclic aromatic hydrocarbon degradation, wastewater denitrification, heavy metal tolerance, and emerging contaminant degradation. In addition, by integrating our research group's work on targeted environmental microorganism isolation devices, programmable logic controller (PLC)-driven ALE systems, and the screening of functional strains for halogenated organic pollutant degradation, this review analyzes the current limitations of these technologies in <i>in situ</i> simulation, maintenance of mixed microbial community interactions, screening of non-growth-coupled phenotypes, and engineering scale-up. Finally, future directions are proposed for the application of automated enrichment and directed acclimation technologies in the exploration, functional enhancement, and engineering application of environmental functional microbial resources. This review is expected to provide theoretical and methodological support for improving the technological framework of environmental functional microorganism development and facilitating its translation into engineering practice.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"3970-3985"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Screening of highly active recombinant bovine enterokinase mutants mediated by green fluorescent protein and its soluble expression in <i>Escherichia coli</i>].","authors":"Changchun Han, Junhao Yue, Yuwen Tu, Zhuhua Chan, Lehe Mei, Changjiang Lyu","doi":"10.13345/j.cjb.260179","DOIUrl":"https://doi.org/10.13345/j.cjb.260179","url":null,"abstract":"<p><p>Bovine enterokinase light chain (EK_L), a serine protease that specifically recognizes the Asp-Asp-Asp-Asp-Lys (DDDDK) sequence, is widely used for the specific cleavage of affinity tags in recombinant proteins. However, EK_L contains multiple cysteine residues and four disulfide bonds, making it difficult to fold correctly, and it tends to form inactive inclusion bodies when expressed in <i>Escherichia coli</i>. To achieve efficient soluble expression of EK_L in <i>E</i>. <i>coli</i> and screen high-activity mutants, co-expression of the DnaK-DnaJ-GrpE and GroES-GroEL molecular chaperones was adopted to enhance its solubility. Subsequently, the DDDDK sequence was site-specifically introduced into the Loop region of superfolder green fluorescent protein (sfGFP) to generate the fluorescent substrate sfGFP^M for EK_L. Based on the principle of fluorescence loss, a high-throughput screening method for highly active EK_L variants was established. Using error-prone PCR, a random mutagenesis library of the <i>EK_L</i> gene was constructed, from which the EK_L-S42M/I139D mutant was obtained. This mutant showed a 2.56-fold increase in catalytic efficiency and significantly improved thermostability. Molecular dynamics simulations revealed that the S42M/I139D mutations synergistically optimized the stability and activity of EK_L<i>via</i> distal allosteric regulation. Meanwhile, fed-batch fermentation further enhanced the soluble expression level of EK_L-S42M/I139D, with a yield of 203 mg/L and an enzymatic activity of 7.45×10⁴ U/mL. This study not only achieves the soluble expression of EK_L in <i>E</i>. <i>coli</i> and obtains a highly active mutant, but also provides a visual tool for the rapid detection of EK_L activity.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"4038-4051"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Stability and viability optimization for filamentous microorganisms in droplet-based microfluidic screening].","authors":"Xueyan Liu, Chenggang Deng, Linlin Qi, Xiaomei Zheng, Ruitong Lv, Yanjiao Zhai, Ran Tu, Lixian Wang, Meng Wang, Jifeng Liu, Yue Zhang","doi":"10.13345/j.cjb.260126","DOIUrl":"https://doi.org/10.13345/j.cjb.260126","url":null,"abstract":"<p><p>Filamentous microorganisms-encompassing filamentous fungi and actinomycetes-are versatile cell factories for producing proteins and bioactive natural products, playing a critical role in the food and pharmaceutical industries. These microorganisms exhibit slow growth and complex morphological differentiation. Conventional screening methods for strain engineering are hindered by low throughput and an inability to resolve heterogeneity in strain cultivation and growth. Droplet-based microfluidics offers an attractive platform for high-throughput screening at the single-cell level, yet its utility is limited by poor droplet stability and low post-sorting viability. Given the aforementioned technical bottlenecks, this study aims to compare the compatibility between filamentous fungi and actinomycetes and droplet microfluidic technology, optimize post-sorting strain viability, and thereby establish a droplet microfluidic screening platform applicable to diverse filamentous microorganisms. Here, we systematically evaluated and optimized droplet-based microfluidic screening for filamentous fungi-<i>Acremonium chrysogenum</i> and two actinomycetes, <i>Streptomyces lividans</i> and <i>Saccharopolyspora erythraea</i>. Monodisperse spore-suspension preparation protocols and droplet incubation time were optimized for each organism, which revealed that actinomycete-generated droplets were significantly more stable than those of filamentous fungi, with optimal sorting windows dictated by strain-specific growth and morphological traits. Subsequently, we varied sorting voltage and recovery regimens (no treatment, demulsification, or demulsification followed by shaking incubation). Lower sorting voltage markedly improved viability, while demulsification effectively boosted viability. These targeted optimizations establish a practical workflow that converts droplet-based microfluidics into a robust tool for high-throughput screening of filamentous microorganisms.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"4225-4238"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}