Sheng wu gong cheng xue bao = Chinese journal of biotechnology最新文献

{"title":"[Genome-wide identification, characterization, and expression analysis of MAPK genes in response to <i>Plasmodiophora brassicae</i> infection in <i>Brassica juncea</i>].","authors":"Chu Xu, Haiping Wang, Jiangping Song, Xiaohui Zhang, Huixia Jia, Jiaqi Han, Zhijie Li, Sen Li, Wenlong Yang","doi":"10.13345/j.cjb.240516","DOIUrl":"https://doi.org/10.13345/j.cjb.240516","url":null,"abstract":"<p><p>In recent years, the spread of clubroot disease caused by <i>Plasmodiophora brassicae</i> infection has seriously affected the yield and quality of <i>Brassica juncea</i> (L.) Czern.. The cascade of mitogen-activated protein kinases (MAPKs), a highly conserved signaling pathway, plays an important role in plant responses to both biotic and abiotic stress conditions. To mine the MAPK genes related to clubroot disease resistance in <i>B</i>. <i>juncea</i>, we conducted a genome-wide analysis on this vegetable, and we analyzed the phylogenetic evolution and gene structure of the MAPK gene family in mustard. The 66 BjuMAPK genes identified by screening the whole genome sequence of <i>B</i>. <i>juncea</i> were unevenly distributed on 17 chromosomes. At the genomic scale, tandem repeats led to an increase in the number of MAPK genes in <i>B</i>. <i>juncea</i>. It was found that members of the same subfamily had similar gene structures, and there were great differences among different subfamilies. These predicted <i>cis</i>-acting elements were related to plant hormones, stress resistance, and plant growth and development. The expression of <i>BjuMAPK02</i>, <i>BjuMAPK15</i>, <i>BjuMAPK17</i>, and <i>BjuMAPK19</i> were down-regulated or up-regulated in response to <i>P</i>. <i>brassicae</i> infection. The above results lay a theoretical foundation for further studying the functions of BjuMAPK genes in <i>B</i>. <i>juncea</i> in response to the biotic stress caused by clubroot disease.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"736-752"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Identification and expression analysis of β-amylase gene family members in alfalfa under saline-alkali stress].","authors":"Hongyu Qu, Lishuang Zhang, Yahui Tang, Lei Liu, Rui Guo, Weileng Guo, Changhong Guo","doi":"10.13345/j.cjb.240801","DOIUrl":"https://doi.org/10.13345/j.cjb.240801","url":null,"abstract":"<p><p>Beta-amylases (BAMs), key enzymes in starch hydrolysis, play an important role in plant growth, development, and resistance to abiotic stress. To mine the saline-alkali tolerance-related BAM genes in alfalfa (<i>Medicago sativa</i> L.), we identified MsBAM genes in the whole genome. The physicochemical properties, phylogeny, gene structures, conserved motifs, secondary structures, promoter <i>cis</i>-acting elements, chromosome localization, and gene replication relationships of BAM gene family members were analyzed. RNA-seq and quantitative real-time PCR (qRT-PCR) were employed to analyze the expression patterns of BAM family members under saline-alkali stress. The results showed that 54 BAM genes were identified in the genome, which were classified into 8 subgroups according to the phylogenetic tree. The members of the same subgroup had similar gene structures except that those of subgroups 1 and 7 had large differences. Conserved motif analysis showed that all MsBAM proteins had a typical glycohydrolysis domain. The chromosome localization analysis showed that MsBAM gene family members were unevenly distributed on 27 chromosomes. The duplication of gene segments led to the increase in BAM gene number in alfalfa. The promoters of BAM genes contained a large number of elements in response to plant hormones and stress. Transcriptome data and qRT-PCR results showed that the expression levels of most MsBAM genes were up-regulated in response to saline-alkali stress. Under the saline-alkali stress, the expression levels of 28 genes, including <i>MsBAM6</i>, were up-regulated on days 1 and 7, and those of 5 genes, including <i>MsBAM9</i>, were up-regulated by over 2 folds. In addition, under salt-alkali stress, BAM activity and soluble sugar content were significantly increased. These results indicate that BAM genes play a key role in alfalfa in response to saline-alkali stress, laying a foundation for further research in this field.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"719-735"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Integrated transcriptomics and metabolomics analysis of flavonoid biosynthesis in <i>Ophiopogon japonicum</i> under cadmium stress].","authors":"Song Gao, Mengli Qiu, Qing Li, Qian Zhao, Erli Niu","doi":"10.13345/j.cjb.240655","DOIUrl":"https://doi.org/10.13345/j.cjb.240655","url":null,"abstract":"<p><p><i>Ophiopogon japonicus</i>, a precious medicinal plant endemic to Zhejiang Province. Its tuberous roots are rich in bioactive components such as flavonoids, possessing anti-inflammatory, antioxidant, and immunomodulatory properties. To elucidate the impact of cadmium (Cd) stress on the accumulation and biosynthetic pathway of flavonoids in <i>O</i>. <i>japonicus</i>, this study exposed <i>O</i>. <i>japonicus</i> to different concentrations of Cd stress and explored the changes through integrated transcriptomics and metabolomics analysis. The results demonstrated that Cd stress (1 mg/L and 10 mg/L) significantly increased the content of flavonoids in <i>O</i>. <i>japonicus</i> in a concentration-dependent manner. The metabolomics analysis revealed a total of 110 flavonoids including flavones, flavanols, flavonols, flavone and flavonol derivatives, flavanones, isoflavonoids, chalcones and dihydrochalcones, and anthocyanins in <i>O</i>. <i>japonicus</i>, among which flavones, flavonols, flavone and flavonol derivatives, and anthocyanins increased under Cd stress. The transcriptomics analysis identified several key flavonoid biosynthesis-associated genes with up-regulated expression under Cd stress, including 14 genes encoding 4-coumarate CoA ligase (4CL), 2 genes encoding chalcone isomerase (CHI), and 14 genes encoding phenylalanine ammonia lyase (PAL). The gene-metabolite regulatory network indicated significant positive correlations of 4CL (<i>Cluster-21637</i>.<i>5012</i>, <i>Cluster-21637</i>.<i>90648</i>, and <i>Cluster-21637</i>.<i>62637</i>) and CHI (<i>Cluster-21637</i>.<i>111909</i> and <i>Cluster-21637</i>.<i>123300</i>) with flavonoid metabolites, suggesting that these genes promoted the synthesis of specific flavonoid metabolites, which led to the accumulation of total flavonoids under Cd stress. These findings provide theoretical support for the cultivation and utilization of medicinal plants in Cd-contaminated environments and offered new perspectives for studying plant responses to heavy metal stress.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"588-601"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Creation of new glabrous and salt-tolerant rice germplasm along the Yellow River by CRISPR-Cas9-mediated editing of <i>OsSPL10</i>].","authors":"Qiangbing Xuan, Huigang Zhou, Minglan Zhu, Junjie Wang, Weihong Liang","doi":"10.13345/j.cjb.240308","DOIUrl":"https://doi.org/10.13345/j.cjb.240308","url":null,"abstract":"<p><p>The <i>OsSPL10</i> gene has previously been reported to positively regulate trichome development and negatively regulate salt and drought stress tolerance in rice. However, it is not clear whether this gene can be used for gene editing to create new germplasm of glabrous leaf and salt-tolerant rice. In this study, we created six rice mutants by CRISPR/Cas9-mediated editing of <i>OsSPL10</i> from 'Xinfeng 2', 'Xinkedao 31', and 'Xindao 25', the main rice cultivars along the Yellow River. Visual observation and scanning electron microscopy verified that the mutants lacked trichomes on the leaves and glumes, and the expression of glabrous marker genes <i>OsHL6</i>, <i>OsGL6</i>, and <i>OsWOX3B</i> in mutants was down-regulated compared with that in the wild type. The net photosynthetic rate, stomatal conductance, and transpiration rate of flag leaves in the mutants were significantly higher than those in the wild type. In addition, the survival rates of the mutants were much higher than that of the wild type after 7 days of treatment with 200 mmol/L NaCl. The results of quantitative real-time polymerase chain reaction (qRT-PCR) further verified that compared with the wild type, the mutants demonstrated down-regulated expression of the salt stress-related gene <i>OsGASR1</i> and up-regulated expression of <i>OsNHX2</i> and <i>OsIDS1</i>. Statistical analysis of agronomic traits showed that the mutants had increased plant height and no significant changes in yield-related traits compared with the wild type. The six <i>spl10</i> mutants created in this study not only had glabrous leaves and glumes but also demonstrated enhanced tolerance to salt stress, serving as new germplasm resources for directional breeding of rice along the Yellow River.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"706-718"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Cloning, prokaryotic expression, and functional validation of flavonoid 3-<i>O</i>-glycosyltransferase gene (<i>Rh3GT</i>) from <i>Rhododendron hybridum</i> Hort].","authors":"Yicheng Yan, Zehang Wu, Yuhang Jiang, Gaoyuan Hu, Yujie Yang, Xiaohong Xie, Yueyan Wu, Yonghong Jia","doi":"10.13345/j.cjb.240614","DOIUrl":"https://doi.org/10.13345/j.cjb.240614","url":null,"abstract":"<p><p>Flavonoid 3-<i>O</i>-glucosyltransferase (3GT) is a key enzyme in the glucosidation of anthocyanins. To investigate the <i>3GT</i> gene in rhododendron, we cloned an open reading frame (ORF) of <i>3GT</i> gene (named <i>Rh3GT</i>) from <i>Rhododendron hybridum</i> Hort (Red cultivar) and then characterized this gene and the deduced protein in terms of the biochemical characteristics, expression level, and enzymatic function. The results showed that <i>Rh3GT</i> had a full length of 993 bp and encoded 330 amino acid residues. The deduced protein was hydrophilic, stable, weak acid, belonging to the glycosyltransferase family (GT-B type), with glutamine (Q) at position 44 in the PSPG box. The phylogenetic analysis showed that <i>Rh3GT</i> was most closely related to <i>Vc3GT</i> from <i>Vaccinium corymbosum</i> and <i>Vm3GT</i> from <i>Vaccinium myrtillus</i>. <i>Rh3GT</i> was expressed in the stems, leaves, and flowers and almost not expressed in the roots, with the highest expression level in petals during full blooming stage. Introduction of pCAMBIAL1302-<i>Rh3GT</i> into petals significantly up-regulated the expression level of <i>Rh3GT</i> and increased the total anthocyanin accumulation. Rh3GT was successfully expressed in <i>Escherichia coli</i> BL21 in the form of inclusion bodies with a size of about 36 kDa. The results of HPLC showed that the recombinant Rh3GT after denaturation, purification, and dilution could catalyze the synthesis of cyanidin and UDP-glucose to synthesize cyanidin 3-<i>O</i>-glucoside, indicating that the expressed protein had 3GT activity. This study provides basic data for further studying the molecular regulation mechanism of anthocyanin biosynthesis and theoretical support for molecular breeding of rhododendron.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"881-895"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Functions and mechanisms of autophagy-related genes in plant responses to adversity stresses].","authors":"Yun'er Ren, Guoqiang Wu, Ming Wei","doi":"10.13345/j.cjb.240661","DOIUrl":"https://doi.org/10.13345/j.cjb.240661","url":null,"abstract":"<p><p>Autophagy is an evolutionarily conserved self-degradation process in eukaryotes. It not only plays a role in plant growth and development but also is involved in plant responses to biotic and abiotic stresses. Plants can initiate autophagy to degrade the surplus or damaged cytoplasmic materials and organelles, thus coping with abiotic and biotic stresses. The initiation of autophagy depends on autophagy-related genes (ATGs). The transcription factors can directly bind to the promoters of ATGs to activate autophagy and regulate their transcriptional levels and post-translational modifications. Furthermore, ATGs can directly or indirectly interact with plant hormones to regulate plant responses to stresses. When plants are exposed to salinity, drought, extreme temperatures, nutrient deficiencies, and pathogen stress, ATGs are significantly induced, which enhances the autophagy activity to facilitate the degradation of the denatured and misfolded proteins, thereby enhancing plant tolerance to adversity stresses. This article summarizes the discovery, structures, and classification of plant ATGs, reviews the research progress in the mechanisms of ATGs in plant responses to abiotic and biotic stresses, and prospects the future research directions. This review is expected to provide the genetic resources and a theoretical foundation for the genetic improvement of crops in responses to stress tolerance.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"510-529"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Genome-wide identification and expression analysis of TCP gene family in <i>Docynia delavayi</i> (Franch.) Schneid.]","authors":"Baoyue Zhang, Guoping Liu, Jinhong Tian, Dawei Wang","doi":"10.13345/j.cjb.240498","DOIUrl":"https://doi.org/10.13345/j.cjb.240498","url":null,"abstract":"<p><p><i>Docynia delavayi</i> (Franch.) Schneid. is an economic fruit plant with high medicinal and edible values. The TCP gene family plays a vital role in plant growth and development. To explore the function of the TCP gene family in the growth and development of <i>D</i>. <i>delavayi</i>. In this study, the TCP gene family (DdeTCP) members were identified from the <i>D</i>. <i>delavayi</i> genome and their expression levels at different stages of seed germination and fruit development were analyzed. The results showed that a total of 18 DdeTCP genes were identified from the <i>D</i>. <i>delavayi</i> genome, with uneven location on 11 chromosomes. The phylogenetic tree showed that the 18 DdeTCPs could be classified into class Ⅱ (3) and class Ⅱ (15), suggesting that functional differentiation occurred among the DdeTCP family members. <i>DdeTCP11</i> highly homologous to <i>AtTCP14</i> was highly expressed in the early stage of seed germination, which suggested that this gene played a key role in seed germination. In addition, <i>DdeTCP16</i> in class Ⅱ had a high expression level during the fruit ripening stage, which indicated that it might be related to fruit ripening. The findings lay a foundation for probing into the roles of the DdeTCP gene family in the growth and development of <i>D</i>. <i>delavayi</i>.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"809-824"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Evaluation of flavonoids in <i>Chimonanthus praecox</i> based on metabolomics and network pharmacology].","authors":"Dan Zhou, Yanbei Zhao, Zixu Wang, Qingwei Li","doi":"10.13345/j.cjb.240548","DOIUrl":"https://doi.org/10.13345/j.cjb.240548","url":null,"abstract":"<p><p>Flavonoids are key bioactive components for evaluating the pharmacological activities of <i>Chimonanthus praecox</i>. Exploring the potential flavonoids and pharmacological mechanisms of <i>C</i>. <i>praecox</i> lays a foundation for the rational development and efficient utilization of this plant. This study employed ultra-performance liquid chromatography-tandem mass spectrometry-based widely targeted metabolomics to comprehensively identify the flavonoids in <i>C</i>. <i>praecox</i>. Network pharmacology was employed to explore the bioactive flavonoids and their mechanisms of action. Molecular docking was adopted to validate the predicted results. Finally, the content of bioactive flavonoids in different varieties of <i>C</i>. <i>praecox</i> was measured. The widely targeted metabolomics analysis identified 387 flavonoids in <i>C</i>. <i>praecox</i>, and the flavonoids varied among different varieties. Network pharmacology predicted 96 chemical components including 19 bioactive compounds, 181 corresponding targets and 2 504 disease targets, among which 99 targets were shared by the active components and the disease. Thirty-three core targets were predicted, involving 229 gene ontology terms and 99 pathways (<i>P</i>≤0.05), which indicated that the flavonoids components of <i>C</i>. <i>praecox</i> exhibited pharmacological activities including antioxidant, anti-inflammatory, antimicrobial, and antiviral activities. Topological analysis screened out five core components (salvigenin, laricitrin, isorhamnetin, quercetin, and 6-hydroxyluteolin) and five core targets (SRC, PIK3R1, AKT1, ESR1, and AKR1C3). The predicted bioactive flavonoids from <i>C</i>. <i>praecox</i> stably bound to key targets, which indicated that these flavonoids possessed potential bioactivities in their interactions with the targets. The flavonoids in <i>C</i>. <i>praecox</i> exerted pharmacological activities in a multi-component, multi-target, and multi-pathway manner. The combined application of metabolomics and network pharmacology provides a theoretical basis for in-depth studies on the pharmacological effects and mechanisms of <i>C</i>. <i>praecox</i>.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"602-617"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Identification and expression pattern analysis of α-glucosidase and β-glucosidase gene family members in melon].","authors":"Yushan Liang, Zhaoyang Zhang, Tingru Yue, Lichao Zhang, Qingjie DU, Jiqing Wang, Huaijuan Xiao, Meng Li","doi":"10.13345/j.cjb.240616","DOIUrl":"https://doi.org/10.13345/j.cjb.240616","url":null,"abstract":"<p><p>Glucosidases are an indispensable class of enzymes in the sugar metabolism of organisms. To investigate the biological functions and expression patterns of α-glucosidases (AGLUs) and β-glucosidases (BGLUs), we identified the two family members in the genome of melon (<i>Cucumis melo</i>). The number, location on chromosomes, gene structure, subcellular localization, conserved motifs, and phylogenetic relationship of the two family members were analyzed. Based on the <i>cis</i>-acting elements in the promoter region and protein interaction models, their functions were preliminarily predicted. Furthermore, the gene expression of the two family members was determined by qRT-PCR. The results showed that the melon genome contained five AGLU family members on five chromosomes, and all of the five members were located in the extracellular matrix, with the amino acid sequence lengths ranging from 899 aa to 1 060 aa. The melon genome carried 18 BGLU family members on 8 chromosomes, and all the members were located in the cell membrane or cytoplasm, with the amino acid lengths ranging from 151 aa to 576 aa. The qRT-PCR results showed that the expression of about 50% of the genes was down-regulated upon cold stress. <i>CmAGLU5</i> and <i>CmBGLU7</i> may be key members of the two families, respectively, in response to cold stress. The expression of all members of the two families was up-regulated under abscisic acid (ABA), high salt, and drought stress. In the AGLU family, <i>CmAGLU3</i> was the key gene in response to ABA and high salt stress, while <i>CmAGLU4</i> was the key gene in response to drought stress. In the BGLU family, <i>CmBGLU18</i> was the key gene in response to ABA, while <i>CmBGLU6</i> was the key gene in response to high salt and drought stress.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"791-808"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Identification of HMA gene family and response to cadmium stress in <i>Ophiopogon japonicas</i>].","authors":"Zhihui Wang, Erli Niu, Yuanliang Gao, Qian Zhu, Zihong Ye, Xiaoping Yu, Qian Zhao, Jun Huang","doi":"10.13345/j.cjb.240475","DOIUrl":"https://doi.org/10.13345/j.cjb.240475","url":null,"abstract":"<p><p>Soil cadmium (Cd) pollution is one of the major environmental problems globally. <i>Ophiopogon japonicus</i>, a multifunctional plant extensively used in traditional Chinese medicine, has demonstrated potential in environmental remediation. This study investigated the Cd accumulation pattern of <i>O</i>. <i>japonicus</i> under cadmium stress and identified the heavy metal ATPase (HMA) family members in this plant. Our results demonstrated that <i>O</i>. <i>japonicus</i> exhibited a Cd enrichment factor (EF) of 2.75, demonstrating strong potential for soil Cd pollution remediation. Nine heavy metal ATPase (HMA) members of P1B-ATPases were successfully identified from the transcriptome data of <i>O</i>. <i>japonicus</i>, with OjHMA1-OjHMA6 classified as the Zn/Co/Cd/Pb-ATPases and OjHMA7-OjHMA9 as the Cu/Ag-ATPases. The expression levels of <i>OjHMA1</i>, <i>OjHMA2</i>, <i>OjHMA3</i>, and <i>OjHMA7</i> were significantly up-regulated under Cd stress, highlighting their crucial roles in cadmium ion absorption and transport. The topological analysis revealed that these proteins possessed characteristic transmembrane (TM) segments of the family, along with functional A, P, and N domains involved in regulating ion absorption and release. Metal ion-binding sites (M4, M5, and M6) existed on the TM segments. Based on the number of transmembrane domains and the residues at metal ion-binding sites, the plant HMA family members were categorized into three subgroups: P1B-1 ATPases, P1B-2 ATPases, and P1B-4 ATPases. Specifically, the P1B-1 ATPase subgroup included the motifs TM4(CPC), TM5(YN[X]₄P), and TM6(M[XX]SS); the P1B-2 ATPase subgroup featured the motifs TM4(CPC), TM5(K), and TM6(DKTGT); the P1B-4 ATPase subgroup contained the motifs TM4(SPC) and TM6(HE[X]GT), all of which were critical for protein functions. Molecular docking results revealed the importance of conserved sequences such as CPC/SPC, DKTGT, and HE[X]GT in metal ion coordination and stabilization. These findings provide potential molecular targets for enhancing Cd uptake and tolerance of <i>O</i>. <i>japonicus</i> by genetic engineering and lay a theoretical foundation for developing new cultivars with high Cd accumulation capacity.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"771-790"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}