Sheng wu gong cheng xue bao = Chinese journal of biotechnology最新文献

{"title":"[Recent progress in ergothioneine of edible fungi].","authors":"Linlei Yang, Zhenhui Shen, Xiangying Luo, Rongping Li, Rongchun Li","doi":"10.13345/j.cjb.240495","DOIUrl":"https://doi.org/10.13345/j.cjb.240495","url":null,"abstract":"<p><p>Ergothioneine is a natural antioxidant known for its potent anti-inflammatory and antioxidative properties. It has been applied in various sectors such as food, cosmetics, and pharmaceuticals. Edible fungi, both wild and cultivated, stand as the primary natural sources capable of synthesizing ergothioneine. This paper reviews the research progress in the content, physiological functions, extraction and detection methods, synthetic genes and pathways, mycelium fermentation, and engineering strain construction for ergothioneine production. The aim is to provide a comprehensive reference for advancing the research and industrial development related to ergothioneine in edible fungi.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"574-587"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Function identification of the mitogen-activated protein kinase gene <i>CsMPK4</i> in cucumber].","authors":"Guanghao Ji, Qianli Lu, Yue Yu, Hebing Wang, Qinglin Tang","doi":"10.13345/j.cjb.240474","DOIUrl":"https://doi.org/10.13345/j.cjb.240474","url":null,"abstract":"<p><p>Cucumber (<i>Cucumis sativus</i> L.) is one of the most widely cultivated vegetables in the world. High temperature and other stress conditions can affect the growth and development of this plant, even leading to the decreases in yield and quality. The mitogen-activated protein kinase (MAPK) family plays a crucial role in plant stress responses. However, the role of <i>MPK4</i> in the stress response of cucumber remains to be reported. In this study, we cloned <i>CsMPK4</i>, which encoded 383 amino acid residues. The qRT-PCR results showed that the expression level of <i>CsMPK4</i> was the highest in leaves and flowers, moderate in roots, and the lowest in stems and tendrils. CsMPK4 was located in the nucleus and cytoplasm, and it had a close relationship with CmMPK4 in muskmelon. The cucumber plants overexpressing <i>CsMPK4</i> became stronger and shorter, with reduced length and quantity of tendrils. Moreover, the transgenic seedlings were more resistant to high temperatures, with decreased malondialdehyde (MDA) content and increased activities of peroxidase (POD) and superoxide dismutase (SOD) in young leaves. Furthermore, the protein-protein interaction between CsMPK4 and CsVQ10, a member of the valine-glutamine family, was confirmed by yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays. The results suggested that CsVQ10 cooperated with CsMPK4 in response to the high temperature stress in cucumber. This study laid a foundation for the further study on the stress response mechanism of <i>CsMPK4</i> and the breeding of stress-resistant cucumber varieties.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"857-868"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Selenium nanoparticles synthesized by <i>Streptomyces avermitilis</i>: physical and chemical characteristics and inhibitory activity on a pathogen of <i>Lycium barbarum</i>].","authors":"Qi Zhang, Yani Li, Rongjuan Zhou, Jiayuan Qing, Sijun Yue","doi":"10.13345/j.cjb.240789","DOIUrl":"https://doi.org/10.13345/j.cjb.240789","url":null,"abstract":"<p><p>Biosynthesized selenium nanoparticles (SeNPs) have attracted much attention because of their unique physical, chemical, and biological properties. The microbial reduction of selenium salts to SeNPs has great potential, while there is a lack of elite strains. In this study, we explored the reduction of Na₂SeO₃ by <i>Streptomyces avermitilis</i> into SeNPs. The colonies and hyphae of the strain and the synthesized SeNPs were characterized by optical microscopy, scanning electron microscopy (SEM), transmission electron microscope (TEM), energy dispersive spectrometry (EDS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). At the same time, the inhibitory activity of SeNPs on <i>Fusarium oxysporum</i>, the main pathogen causing root rot of <i>Lycium barbarum</i>, was studied. The results showed that <i>S</i>. <i>avermitilis</i> converted Na₂SeO₃ into SeNPs and tolerated 300 mmol/L Na₂SeO₃, demonstrating strong tolerance. <i>S</i>. <i>avermitilis</i> synthesized spherical SeNPs in the cytoplasm, and most of SeNPs had a diameter of about 100 nm and were released by hyphal fracture. The SeNPs synthesized by <i>S</i>. <i>avermitilis</i> were amorphous, and their surfaces were dominated by C and Se, with the existence of O, N and other elements. SeNPs had functional groups such as -OH, C=O, C-N, and C-H, which were closely related to the stability and biological activity of SeNPs. The SeNPs synthesized by <i>S</i>. <i>avermitilis</i> showcased significant inhibitory activity on <i>F</i>. <i>oxysporum</i>, and 25.0 μmol/mL SeNPs showcased the inhibition rate of 77.61% and EC₅₀ of 0.556 μmol/mL. In conclusion, <i>S</i>. <i>avermitilis</i> can tolerate high Na₂SeO₃ stress and mediate the synthesis of SeNPs. The synthesized SeNPs have good stability and strong inhibitory activity, demonstrating the potential application value in the preparation of SeNPs and the control of <i>L</i>. <i>barbarum</i> root rot.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"693-705"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Genetic diversity and molecular identity of <i>Prunus mume</i> with both ornamental and edible values based on fluorescence-labeled simple sequence repeat (SSR) markers].","authors":"Zixu Wang, Dan Zhou, Yanbei Zhao, Yuhang Tong, Weijun Zheng, Qingwei Li","doi":"10.13345/j.cjb.240676","DOIUrl":"https://doi.org/10.13345/j.cjb.240676","url":null,"abstract":"<p><p>We studied the genetic diversity and established the DNA molecular identify for <i>Prunus mume</i> with both ornamental and edible values, aiming to collect, identify, evaluate, and breed new varities of this plant and promote the upgrading of the <i>P</i>. <i>mume</i> industry chain in northern China. We employed 13 pairs of primers with good polymorphism, clear bands, and good repeatability to analyze the genetic diversity and establish the molecular identify of 68 germplasm accessions of <i>P</i>. <i>mume</i> with both ornamental and edible values from Xingtai, Hebei Province. We then employed the unweighted pair-group method with arithmetic means (UPGMA) to perform the cluster analysis based on genetic distance. After that, we analyzed the genetic structure of the 68 germplasm accessions based on a Bayesian model. The 13 pairs of SSR primers amplified a total of 124 alleles from 68 <i>P</i>. <i>mume</i> germplasm accessions, with the mean number of alleles (<i>Na</i>) of 9.538 5, the minor allele frequency (<i>MAF</i>) of 0.369 3, the mean number of effective alleles (<i>Ne</i>) of 4.483 5, and the mean Shannon genetic diversity index (<i>I</i>) of 1.712 4. The mean Nei's gene diversity index (<i>H</i>) of 0.763 7, the mean observed heterozygosity (<i>Ho</i>) of 0.719 5, the mean expected heterozygosity (<i>He</i>) of 0.769 3, the mean polymorphism information content (<i>PIC</i>) of 0.733 6, and the mean genetic similarity (<i>GS</i>) of 0.772 9 suggested that there were significant genetic differences and rich genetic diversity among the studied <i>P</i>. <i>mume</i> germplasm accessions. The cluster analysis revealed that the 68 accessions were classified into three groups, with the mean genetic distance of 0.622 6. The population structure analysis classified the germplasm accessions into two populations. According to the <i>PIC</i> of primers, we selected primers for combination and constructed the combination with the fewest primers required for germplasm differentiation of <i>P</i>. <i>mume</i> with both ornamental and edible values. This study provides a theoretical basis for the innovation and industrial upgrading of <i>P</i>. <i>mume</i> with both ornamental and edible values in gardening and the improvement of breeding efficiency.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"639-656"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Identification of the MYB transcription factor family involved in response to salt stress in <i>Picea mongolica</i>].","authors":"Mingming Sui, Fuman Zhang, Tian Tian, Yanqiu Yan, Le Geng, Hui Li, Yu'e Bai","doi":"10.13345/j.cjb.240699","DOIUrl":"https://doi.org/10.13345/j.cjb.240699","url":null,"abstract":"<p><p><i>Picea mongolica</i>, known for its remarkable tolerance to cold, drought, and salinity, is a key species for ecological restoration and urban greening in the \"Three Norths\" region of China. MYB transcription factors are involved in plant responses to abiotic stress and synthesis of secondary metabolites. However, studies are limited regarding the MYB transcription factors in <i>P</i>. <i>mongolica</i> and their roles in salt stress tolerance. In this study, 196 MYBs were identified based on the genome of <i>Picea abies</i> and the transcriptome of <i>P</i>. <i>mongolica</i>. Phylogenetic analysis classified the MYB transcription factors into seven subclasses. The R2R3-MYB subclass contained the maximum number of genes (84.77%), while the R-R and R1R2R3 subclasses each represented the smallest proportion, at about 0.51%. The MYB transcription factors within the same subclass were highly conserved, exhibiting similar motifs and gene structures. Experiments with varying salt stress gradients revealed that <i>P</i>. <i>mongolica</i> could tolerate the salt concentration up to 1 000 mmol/L. From the transcriptome data of <i>P</i>. <i>mongolica</i> exposed to salt stress (1 000 mmol/L) for 0, 3, 6, 12, and 24 h, a total of 34 differentially expressed MYBs were identified, which suggested that these MYBs played a key role in regulating the response to salt stress. The proteins encoded by these differentially expressed genes varied in length from 89 aa to 731 aa, with molecular weights ranging from 10.19 kDa to 79.73 kDa, isoelectric points between 4.80 and 9.91, and instability coefficients from 41.20 to 70.99. Subcellular localization analysis indicated that most proteins were localized in the nucleus, while three were found in the chloroplasts. Twelve MYBs were selected for quantitative real-time PCR (qRT-PCR), which showed that their expression patterns were consistent with the RNA-seq data. This study provides valuable data for further investigation into the functions and mechanisms of MYB family members in response to salt stress in <i>P</i>. <i>mongolica</i>.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"825-844"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress in the engineering strains for producing double-stranded RNA].","authors":"Jincheng Cui, Jie Cui, Xiaoying Bian","doi":"10.13345/j.cjb.240571","DOIUrl":"https://doi.org/10.13345/j.cjb.240571","url":null,"abstract":"<p><p>Ensuring food security requires new green pesticides. Double-stranded RNA (dsRNA) pesticides trigger RNA interference by exogenous dsRNA specifically targeting pests and diseases. They can inhibit the expression of key genes in pathogens or pests, thereby achieving effective control of specific pests and diseases. DsRNA pesticides are environmentally friendly, with strong specificity and efficient gene silencing ability, while they have problems such as high production costs. Using engineering strains to produce dsRNA is a feasible strategy, whereas currently there is no cost-effective engineering strain for producing dsRNA. This article reviews the research progress and production strategies of using microorganisms to produce dsRNA, hoping to provide reference for dsRNA production.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"546-558"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Advances in genetic engineering and molecular modification of sweet-tasting proteins].","authors":"Shangyang Lu, Shiyu Chang, Yuqing Wang, Bo Liu","doi":"10.13345/j.cjb.240123","DOIUrl":"https://doi.org/10.13345/j.cjb.240123","url":null,"abstract":"<p><p>Sweet-tasting proteins demonstrate application potential in foods and beverages due to their high sweetness, low calorie, and non-toxicity. So far, eight natural sweet-tasting proteins have been obtained from natural plants. This paper briefs the sweetness properties of the eight proteins and the molecular mechanism of the sweetness, reviews the progress in the genetic engineering, heterologous expression, and molecular modification of three representative sweet-tasting proteins (monellin, brazzein, and thaumatin), and summarizes their expression yields in different hosts and sweetness properties. Lastly, this paper prospects the research, application, and industrial development of sweet-tasting proteins. This review provides a reference for further research and development of new proteinaceous sweeteners.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"559-573"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Cloning and functional characterization of <i>PhNAL1b</i> from <i>Petunia</i>× <i>hybrida</i> cv. Mitchell Diploid].","authors":"Xurong Yao, Tongrui Liu, Lili Dong, Xinyi Deng","doi":"10.13345/j.cjb.240466","DOIUrl":"https://doi.org/10.13345/j.cjb.240466","url":null,"abstract":"<p><p><i>Narrow leaf 1</i> (<i>NAL1</i>) plays an important role in plant branching, while little is known about the roles of this gene in petunias. In this study, <i>PhNAL1b</i> was cloned from <i>Petunia</i>×<i>hybrida</i> cv. Mitchell Diploid, with a total length of 1 767 bp, encoding a protein composed of 588 amino acid residues and containing the peptidase S64 domain. The <i>PhNAL1b</i> promoter region contained several elements involved in the responses to auxin, jasmonic acid, abscisic acid, and light. The expression analysis showed that <i>PhNAL1b</i> had the highest expression level in roots and the lowest expression level in flowers, and its transcription could be inhibited by decapitation and cytokinin. The subcellular localization analysis showed that PhNAL1b was located in the nucleus and was a nuclear protein. Virus-induced gene silencing was employed to downregulate the expression of <i>PhNAL1b</i>, which resulted in significant increases in branch number and plant height. The results indicated that <i>PhNAL1b</i> played an important role in regulating the branching of petunias. This study lays a foundation for revealing the mechanism of <i>NAL1</i> in regulating branch development and provides genetic resources for plant architecture improvement.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"869-880"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Functional analysis of a nitrate-induced GARP transcription factor AhNIGT1.2 in peanut nodulation].","authors":"Xiaoliang Li, Haitong He, Suqin He, Luyao Wang, Wei Zhang, Zhaosheng Kong, Lixiang Wang","doi":"10.13345/j.cjb.240407","DOIUrl":"https://doi.org/10.13345/j.cjb.240407","url":null,"abstract":"<p><p>Peanut, a major economic and oil crop known for the high protein and oil content, is extensively cultivated in China. Peanut plants have the ability to form nodules with rhizobia, where the nitrogenase converts atmospheric nitrogen into ammonia nitrogen that can be utilized by the plants. Analysis of nodule fixation is of positive significance for avoiding overapplication of chemical fertilizer and developing sustainable agriculture. In this study, <i>AhNIGT1</i>.<i>2</i>, a member of the <i>NIGT</i> family predominantly expressed in peanut nodules, was identified by bioinformatics analysis. Subsequent spatiotemporal expression analysis revealed that <i>AhNIGT1</i>.<i>2</i> was highly expressed in nodules and showed significant responses to high nitrogen, low nitrogen, high phosphorus, low phosphorus, and rhizobia treatments. Histochemical staining indicated that the gene was primarily expressed in developing nodules and at the connection region between mature nodules and peanut roots. The fusion protein AhNIGT1.2-GFP was located in the nucleus of tobacco epidermal cells. The <i>AhNIGT1</i>.<i>2</i>-OE significantly increased the number of peanut nodules, while <i>AhNIGT1</i>.<i>2</i>-RNAi reduced the number of nodules, which suggested a positive regulatory role of AhNIGT1.2 in peanut nodulation. The <i>AhNIGT1</i>.<i>2</i>-OE in roots down-regulated the expression levels of <i>NRT1</i>.<i>2</i>, <i>NRT2</i>.<i>4</i>, <i>NLP1</i>, and <i>NLP7</i>, which indicated that <i>AhNIGT1</i>.<i>2</i> influenced peanut nodulation by modulating nitrate transport and the expression of <i>NLP</i> genes. The transcriptome analysis of <i>AhNIGT1</i>.<i>2</i>-OE and control roots revealed that overexpressing <i>AhNIGT1</i>.<i>2</i> significantly enriched the differentially expressed genes associated with nitrate response, nodulation factor pathway, enzymes for triterpene biosynthesis, and carotenoid biosynthesis. These findings suggest that <i>AhNIGT1</i>.<i>2</i> play a key role in peanut nodulation by regulating nitrate transport and response and other related pathways. This study gives insights into the molecular mechanisms of nitrogen and phosphorus in regulating legume nodulation and nitrogen fixation, and sheds light on the development of legume crops that can efficiently fix nitrogen in high nitrogen environments.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"657-669"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Functions and mechanisms of Zn²⁺-dependent histone deacetylase in plant responses to abiotic stress].","authors":"Ming Wei, Meng Zhao, Xinrui Wu, Guoqiang Wu","doi":"10.13345/j.cjb.240570","DOIUrl":"https://doi.org/10.13345/j.cjb.240570","url":null,"abstract":"<p><p>The HDAs (a subfamily of histone deacetylases), a class of Zn²⁺-dependent histone deacetylases, are highly homologous to the reduced potassium dependency 3 (RPD3) in yeast. HDAs extensively regulate chromosome stability, gene transcription, and protein activity by catalyzing the removal of acetyl group from histone and non-histone lysine residues. HDA-mediated deacetylation is essential for plant growth, development, and responses to abiotic stress. We review the research progress in HDAs regarding the discovery, structures, classification, deacetylation process, and roles in regulating plant responses to abiotic stress. Furthermore, this paper prospects the future research on HDAs, aiming to provide theoretical support for the research on epigenetic regulation mediated by HDAs.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"41 2","pages":"491-509"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}