Sheng wu gong cheng xue bao = Chinese journal of biotechnology最新文献

{"title":"[Construction of an anti-phage network system based on synergistic mechanisms and its applications in synthetic biology].","authors":"Zan Zhang, Hengwei Zhang, Jiajia You, Zhiming Rao","doi":"10.13345/j.cjb.260142","DOIUrl":"https://doi.org/10.13345/j.cjb.260142","url":null,"abstract":"<p><p>In natural ecosystems, bacteriophages play a crucial role in bacterial lysis. Bacteriophage contamination frequently leads to fermentation failure, resulting in compromised product quality and purity, as well as substantial economic loss. Consequently, effective strategies are required in the fermentation industry to mitigate bacteriophage contamination. The use of a single anti-phage strategy is readily evaded by spontaneous phage mutations, resulting in limited and poorly applicable protection against phage infection. Synergistic defense mechanisms involving multiple systems suppress the spontaneous mutations that enable phage escape, thereby broadening phage resistance. This study is designed to tackle the bottleneck associated with single anti-phage strategies, which are susceptible to escape <i>via</i> spontaneous phage mutations, exhibit limited protective efficacy, and suffer from poor applicability. By integrating receptor engineering with synergistic defense mechanisms, a highly efficient, broad-spectrum, and non-toxic phage defense network system is constructed. This system is subsequently applied to industrial fermentation strains, with the aim of achieving effective resistance against phage contamination and improving the stability of fermentation processes. Initially, a synergistic resistance mechanism between Shedu and Septu was identified through laboratory screening of defense mechanisms. Further investigation revealed that the endonuclease activity of Shedu and the ATPase and HNH nuclease activities of Septu jointly contribute to synergistic resistance. Optimization of promoters was employed to adapt the Shedu and Septu mechanisms, thereby conferring resistance to phages T4, λ, P251, and P255. Subsequently, receptor engineering was applied to modify <i>fhuA</i>, a key adsorption site for phages T1 and T7, to achieve resistance against these phages and complete the construction of the anti-phage network system. Growth curve analysis demonstrated that the anti-phage network system exhibits minimal toxicity and high resistance efficiency, highlighting its strong industrial application potential. Finally, phage resistance was introduced into riboflavin- and ʟ-leucine-producing strains, enabling them to effectively counter phage contamination during fermentation. In a 5 L bioreactor, riboflavin production reached 11.2 g/L and ʟ-leucine production reached 64.0 g/L, thereby confirming the industrial potential of the anti-phage system. The resistance strategy reported herein facilitates the development of engineered <i>Escherichia coli</i> strains with robust and broad-spectrum phage resistance for using in the industrial fermentation of natural amino acids and high-value compounds.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"3999-4017"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Medium optimization and fermentation regulation strategies for enhancing CO₂ conversion to acetic acid coupled with H₂ by <i>Clostridium ljungdahlii</i>].","authors":"Shaochong Li, Hang Su, Xinyu Guo, Jingyan Chen, Fuli Li, Ming Lyu","doi":"10.13345/j.cjb.260275","DOIUrl":"https://doi.org/10.13345/j.cjb.260275","url":null,"abstract":"<p><p><i>Clostridium ljungdahlii</i> can reduce CO₂ to acetic acid <i>via</i> the Wood-Ljungdahl pathway using H₂ as an energy source and CO₂ as a carbon source, and it is a key chassis for realizing resource utilization of CO₂. Acetic acid, as a bulk basic organic chemical raw material, has a large market demand and wide application scenarios. Moreover, the syngas fermentation route is green and low-carbon, with mild reaction conditions, making it an ideal direction for replacing traditional petroleum-based processes. However, during the fermentation process, it encountered problems such as slow growth, low biomass, and low acetic acid production. Therefore, in this study, the strain <i>C</i>. <i>ljungdahlii</i> SL40, which was obtained through laboratory adaptive evolution, was selected as the object of investigation. Through optimization of the culture medium and regulation of key fermentation processes, its acetic acid synthesis capacity was enhanced. Single-factor experiments integrated with response surface methodology were used to optimize the culture conditions. The optimal parameters were determined as follows: H₂: CO₂=60%: 40% (<i>V</i>/<i>V</i>), yeast extract 4.0 g/L, FeSO₄ 16 mg/L, culture temperature 37℃. Further experiments were conducted in a 5 L fermentation tank to investigate the effects of aeration rate and pH on the product. The results showed that increasing the aeration rate from 0.8 L/min to 1.5 L/min significantly promoted the production of acetic acid, raising the acetic acid concentration from 20.740 g/L to 25.950 g/L. When pH was controlled at 5.85, the acetic acid concentration was further increased to 36.570 g/L. Through systematic optimization, this study effectively broke through the key bottlenecks such as low biomass and low gas utilization rate in the autotrophic fermentation of the strain and significantly improved the acetic acid yield. These findings provide a feasible path for the directed conversion of CO₂ to produce high value-added chemicals and has good industrial application potential.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"4271-4288"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Data-mechanism integrated optimization and preliminary scale-up study of feeding process in penicillin fermentation].","authors":"Yanjun Lu, Hongtao Zhu, Tao Feng, Lihuan Su, Hu Fan, Qingchao Jiang, Yingping Zhuang, Guan Wang","doi":"10.13345/j.cjb.260249","DOIUrl":"https://doi.org/10.13345/j.cjb.260249","url":null,"abstract":"<p><p>With the advancement of online monitoring and automation in industrial fermentation, large volumes of production operation data have been accumulated, while their potential value in fermentation regulation and process optimization has not been fully exploited. This study aims to investigate the key causes of titer variation in industrial-scale penicillin fermentation, thereby guiding the optimization of the feeding process. To this end, industrial historical data was analyzed to elucidate the relationships between key process variables and production performance, and to further investigate and validate their physiological regulatory significance. The production titer at harvest, maximum specific production rate (q P, m a x), and initiation timing of secondary metabolism were selected as characterization indicators. Curve similarity and correlation analyses were performed for staged screening of the data of 325 production batches from a 156 m³ industrial fermenter. The results indicated that cumulative glucose feeding amount and dilution rate were the key factors contributing to variations in fermentation performance. Further analysis revealed that a high dilution rate during the late fermentation stage maintained a high specific growth rate (μ), thereby limiting the accumulation of penicillin, a typical secondary metabolite. In contrast, low glucose feeding and reduced dilution rates promoted a decline in μ and enhanced production capacity, quantitatively demonstrating, at the industrial scale, the regulatory role of μ in secondary metabolism. On the basis of these findings, a viable cell electrode was introduced into a 50-L fermentation system to enable online biomass monitoring, and physiological metabolic parameters were used to reproduce industrial fermentation states. Subsequently, dynamic regulation of μ during the production phase was achieved by increasing the feed glucose concentration and optimizing the feeding rate to reduce late-stage dilution rate. Laboratory-scale experiments showed that the glucose conversion efficiency, titer, and fermentation index increased by 22.06%, 16.10%, and 16.42%, respectively. Preliminary scale-up results based solely on increasing feed glucose concentration demonstrated a 6.56% increase in titer, a 4%-5% improvement in overall production, and a 2.20% increase in fermentation index. This study developed a data-mechanism integrated strategy for fermentation process optimization, providing a new approach for precise regulation and optimization of large-scale biomanufacturing processes.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"4239-4255"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of combined CaO-CO₂ detoxification treatment on the conversion of sugarcane bagasse enzymatic hydrolysate to xanthan gum].","authors":"Zecheng Wan, Yunkai Zhang, Qingyan Wang, Xiaojuan Liang, Chunxiao Zhao, Guanghao Fu, Xiaobei Zhan, Hongtao Zhang","doi":"10.13345/j.cjb.260202","DOIUrl":"https://doi.org/10.13345/j.cjb.260202","url":null,"abstract":"<p><p>Replacing grain-derived carbon sources with non-grain biomass carbon sources for fermentation is a core research direction in the field of biomanufacturing. However, inhibitory byproducts present in the enzymatic hydrolysate of non-grain biomass severely restrict its practical application. We aim to reduce the dependence of xanthan gum fermentation on grain-based carbon sources, clarify the impacts of typical inhibitors from non-grain biomass enzymatic hydrolysate on xanthan gum synthesis, break through the existing application bottlenecks, and develop a green, low-loss and high-efficiency detoxification technology to overcome the inherent defects of traditional detoxification processes. In this study, we used sugarcane bagasse enzymatic hydrolysate as the fermentation carbon source, and the xanthan gum-producing strain <i>Xanthomonas campestris</i> 1.178 as the research object. We investigated the effects of furfural (FFR) and 5-hydroxymethylfurfural (HMF) on xanthan gum biosynthesis. To address the defects of high sugar loss and massive byproduct generation in the conventional CaO-H₃PO₄ detoxification process, we developed an innovative CaO-CO₂ combined detoxification process, and comparatively analyzed the physicochemical properties of xanthan gum produced <i>via</i> the two detoxification processes. We identified that acetic acid and isovaleric acid are the key inhibitory factors in sugarcane bagasse enzymatic hydrolysate that limit xanthan gum synthesis. After CaO-CO₂ treatment, we achieved retention rates of 98.23% for total sugar, 95.63% for reducing sugar, and 95.08% for glucose in the hydrolysate. We obtained a xanthan gum yield as high as 19.76 g/L, which was 19.75% higher than the yield from the CaO-H₃PO₄ treatment. The xanthan gum prepared in this study shared consistent infrared spectrum characteristics with commercially available xanthan gum, and we confirmed its excellent tolerance under low temperature, alkaline and high-salt conditions. The CaO-CO₂ detoxification process developed in this study realizes efficient conversion of sugarcane bagasse enzymatic hydrolysate to xanthan gum, and provides a brand-new green solution for the high-value utilization of non-grain biomass.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"4210-4224"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Advances and applications of microbial genome evolution engineering].","authors":"Zhimin Han, Lian Wang, Lixia Fang, Yingxiu Cao","doi":"10.13345/j.cjb.260147","DOIUrl":"https://doi.org/10.13345/j.cjb.260147","url":null,"abstract":"<p><p>The construction of microbial cell factories frequently encounters bottlenecks due to the complexity of metabolic pathways, insufficient enzyme functionality, and poor environmental tolerance. Directed evolution, as a pivotal technology for overcoming these engineering constraints, can significantly enhance the titer, productivity, and overall robustness of target-producing strains. Its development originated from traditional random mutagenesis, a method that generates genetic diversity through the imposition of artificial growth pressures and is currently in widespread use. However, owing to the uncontrollable direction of mutagenesis and the low frequency of beneficial mutations, this approach suffers from low screening efficiency and is both time-consuming and labor-intensive. To accelerate the evolutionary rate, global accelerated evolution strategies based on tools such as DNA repair deficiencies and cytidine deaminases have emerged, achieving orders-of-magnitude increases in genome-wide mutation rates. Targeted continuous evolution strategies centered on CRISPR/Cas and T7 RNA polymerase systems enable the precise introduction of mutations within predefined genomic regions, thereby greatly improving the enrichment efficiency of beneficial mutations while minimizing non-productive mutations. This review will focus on three core strategies-traditional random evolution, global accelerated evolution, and targeted continuous evolution-systematically elucidating their developmental trajectories, applications in the construction of microbial cell factories, and future directions. Collectively, this review aims to provide methodological guidance for overcoming the engineering bottlenecks in the construction of microbial cell factories.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"3905-3923"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Recent advances in the assembly and functional applications of synthetic genomes].","authors":"Long Zhao, Biyifan Wang, Bin Jia","doi":"10.13345/j.cjb.260267","DOIUrl":"https://doi.org/10.13345/j.cjb.260267","url":null,"abstract":"<p><p>With the development of DNA synthesis and genome engineering technology, life science research has gradually evolved from gene editing to higher-level genome writing. By designing and constructing large-scale DNA fragments and even complete genomes from scratch, researchers have been able to reconstruct the genetic structures of life systems at the genome scale, which enables the systematical study of the relationship between genome structure and function. Related studies have achieved remarkable progress in various biological systems such as prokaryotes, <i>Saccharomyces cerevisiae</i>, and mammalian cells and have shown broad application prospects of this technology in functional gene screening, biological manufacturing, medical research, and basic life science research. This paper systematically reviews the main progress in large-scale DNA design and assembly technology, summarizes the research progress of synthetic genomes in different biological systems and their potential in functional gene screening and biotechnology applications, and discusses the current technical challenges and future development directions, aiming to provide a reference for the research and application of synthetic genome engineering.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"3924-3944"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Recent progress in algal-bacterial symbiotic and mutualistic systems].","authors":"Shuxian Lu, Dailin Liu, Yubei Chen, Qiman Sun, Lei Chen, Tao Sun, Weiwen Zhang","doi":"10.13345/j.cjb.260133","DOIUrl":"https://doi.org/10.13345/j.cjb.260133","url":null,"abstract":"<p><p>Algal-bacterial symbiotic and mutualistic systems enhance community stability and functional performance through interspecies cooperation and signal exchange, demonstrating considerable potential in environmental remediation and sustainable biomanufacturing. This review systematically summarizes the fundamental interaction types and underlying mechanisms of algal-bacterial symbiosis and mutualism, with a particular focus on enabling technologies for the rational reconstruction of engineered mutualistic consortia and their emerging applications in environmental remediation and biomanufacturing. Different interaction modes, including mutualism, commensalism, parasitism, and endosymbiosis, are comparatively discussed, together with key regulatory mechanisms such as chemical signaling, nutrient exchange, horizontal gene transfer, and homeostasis maintenance under abiotic stress conditions. At the technical level, this paper introduces the recent advances in single-cell dynamic monitoring approaches, artificial intelligence-driven design platforms, and synthetic community engineering strategies integrating both top-down and bottom-up methodologies. Furthermore, this paper discusses the potential applications of algal-bacterial mutualistic systems in wastewater remediation, aquaculture, bioenergy recovery, and sustainable bioproduction and points out the current research challenges related to mechanism understanding, species specificity, long-term system stability, and ecological risk assessment. Finally, this review proposes that interdisciplinary approaches integrating multi-omics analyses, computational modeling, and controllable environmental validation will facilitate the development of efficient, robust, and sustainable algal-bacterial symbiotic systems, thereby providing theoretical guidance and technical support for advancing synthetic biology-driven resource utilization and green biomanufacturing.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"3884-3904"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Multidimensional metabolic engineering of <i>Yarrowia lipolytica</i> for β-carotene production].","authors":"Yue Li, Lu Shan, Min Chen, Qi Liu, Peipei Han, Zongjie Dai, Qinhong Wang","doi":"10.13345/j.cjb.260398","DOIUrl":"https://doi.org/10.13345/j.cjb.260398","url":null,"abstract":"<p><p>β-carotene is a natural lipid-soluble carotenoid widely used in the food and pharmaceutical industries. Although metabolic engineering efforts for the production of β-carotene <i>via</i> microbial fermentation have achieved certain progress, there remains room for further improvement. Compared with <i>Saccharomyces cerevisiae</i> or <i>Escherichia coli</i>, <i>Yarrowia lipolytica</i> exhibits a pronounced lipid-flux orientation in its core metabolic logic, conferring a natural advantage for the production of lipid-soluble compounds. This study aims to engineer <i>Y</i>. <i>lipolytica</i> strains for efficient β-carotene production. First, heterologous synthesis of β-carotene was achieved by replacing native promoters with strong ones, attenuating competing pathways, and introducing a bifunctional enzyme derived from <i>Mucor circinelloides</i>. Subsequently, β-carotene production was enhanced through morphological engineering to inhibit hyphal formation, optimization of key gene copy numbers, and improvement of acetyl-CoA supply. The resulting engineered strain LY20 achieved a β-carotene titer of 346.2 mg/L in shake-flask fermentation. Furthermore, in fed-batch fermentation with glucose as the carbon source in a 7.5-L bioreactor, the β-carotene titer reached 3.56 g/L. This study systematically optimizes the biosynthetic pathway for β-carotene through systems metabolic engineering, highlighting the potential of <i>Y</i>. <i>lipolytica</i> for the engineered synthesis of highly hydrophobic natural products and providing guidance for the efficient production of other lipid-soluble terpenoids.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"4052-4066"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Efficient production of l- lysine by <i>Escherichia coli</i> through systems metabolic engineering].","authors":"Haomiao Wang, Liming Liu, Jia Liu, Xiaomin Li","doi":"10.13345/j.cjb.260168","DOIUrl":"https://doi.org/10.13345/j.cjb.260168","url":null,"abstract":"<p><p>As an essential amino acid, l- lysine is widely used in the feed, food, and pharmaceutical industries. However, the production of l- lysine <i>via</i><i>Escherichia coli</i> fermentation is still plagued by inadequate substrate utilization, severe carbon atom waste, and a low sugar-to-acid conversion rate. To address these issues, this study used <i>E</i>. <i>coli</i> M2019435 as the chassis strain to generate strain HM-01 by knocking out the global regulatory factor gene <i>mlc</i> and heterologously expressing <i>malp</i> and <i>glvA</i> derived from <i>Bacillus subtilis</i> as well as the sucrose metabolism gene cluster <i>scrKYAB</i>. On this basis, carbon-13 labeled metabolic flux analysis (¹³C-MFA) was employed to identify the bottlenecks of pyruvate accumulation and carbon atom loss. The pyruvate carboxylase gene <i>pyc</i> and carbonic anhydrase gene <i>cynT</i> were then introduced to construct a CO₂ refixation loop. Meanwhile, feedback inhibition was relieved and the l- lysine degradation gene <i>cadA</i> was knocked out, resulting in the generation of strain HM-05. Further optimization of the residual sugar concentration (0.50 g/L) and carbon-to-nitrogen ratio (1:1 to 1:2) during the fermentation of strain HM-05 was conducted in a 5-L fermenter to achieve the precise supply of carbon and nitrogen sources. After 30 h of fermentation, the l- lysine titer, sugar-to-acid conversion rate, and productivity reached 210.8 g/L, 0.768 g/g, and 7.02 g/(L · h), which were 44.8%, 27.5%, and 42.7% higher than those of the original strain, respectively. The strategy of enhanced substrate utilization-targeted carbon flux optimization-upgraded process optimization developed in this study provides important support for the industrial production of l- lysine.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"4089-4105"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Efficient fermentation process for rhamnolipid production based on machine vision and Bayesian optimization].","authors":"Yang Yu, Xiaogang Wang, Jiayu Lei, Fusheng Lujin, Xiaohu Luo, Mingyu Deng, Zhenghong Xu, Jinsong Shi, Heng Li","doi":"10.13345/j.cjb.260221","DOIUrl":"https://doi.org/10.13345/j.cjb.260221","url":null,"abstract":"<p><p>Rhamnolipids, a class of biosurfactants, are widely applied in industries such as petrochemicals, environmental agriculture, daily chemicals and pharmaceuticals. However, their industrial application is severely constrained by the low rhamnolipid yield of natural strains and high overall production costs. To obtain microbial strains with high rhamnolipid-producing capacity and to enhance their fermentation yields, this study developed a screening method for rhamnolipid-producing microorganisms assisted by the oil spreading technique based on machine vision (MV). Using this approach, a <i>Pseudomonas aeruginosa</i> strain PA022 with favorable rhamnolipid-producing capacity was isolated from an environmental soil sample. Based on single-factor preliminary experiments, Bayesian optimization (BO) coupled with Latin hypercube sampling (LHS) was applied to perform global optimization of key fermentation parameters, thereby determining the medium composition and culture conditions for PA022 as follows: rapeseed oil 30 g/L, NaNO₃ 12 g/L, peptone 3 g/L, phosphate at a ratio of 3:1 with a total concentration of 1.2 g/L, initial pH 6.8, temperature 35℃, and inoculum size 2.5%. Under the optimal fermentation conditions, strain PA022 achieved a rhamnolipid titer of 20.01 g/L in shake-flask fermentation, and the yield reached 30.52 g/L at the 5 L fermenter scale. This study demonstrates that the small-sample global optimization strategy combining MV and BO can enhance the efficiency of strain screening and fermentation process development, thereby providing a reference for the optimization of rhamnolipid and analogous fermentation processes.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 9","pages":"4256-4270"},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}