Science AdvancesPub Date : 2025-04-23DOI: 10.1126/sciadv.adq6077
Laurent Guillaud, Anna Garanzini, Sarah Zakhia, Sandra De la Fuente, Dimitar Dimitrov, Susan Boerner, Marco Terenzio
{"title":"Loss of intracellular ATP affects axoplasmic viscosity and pathological protein aggregation in mammalian neurons","authors":"Laurent Guillaud, Anna Garanzini, Sarah Zakhia, Sandra De la Fuente, Dimitar Dimitrov, Susan Boerner, Marco Terenzio","doi":"10.1126/sciadv.adq6077","DOIUrl":"10.1126/sciadv.adq6077","url":null,"abstract":"<div >Neurodegenerative diseases display synaptic deficits, mitochondrial defects, and protein aggregation. We show that intracellular adenosine triphosphate (ATP) regulates axoplasmic viscosity and protein aggregation in mammalian neurons. Decreased intracellular ATP upon mitochondrial inhibition leads to axoterminal cytosol, synaptic vesicles, and active zone component condensation, modulating the functional organization of mouse glutamatergic synapses. Proteins involved in the pathogenesis of Parkinson’s disease (PD), Alzheimer’s disease (AD), and amyotrophic lateral sclerosis (ALS) condensed and underwent ATP-dependent liquid phase separation in vitro. Human inducible pluripotent stem cell–derived neurons from patients with PD and ALS displayed reduced axoplasmic fluidity and decreased intracellular ATP. Last, nicotinamide mononucleotide treatment successfully rescued intracellular ATP levels and axoplasmic viscosity in neurons from patients with PD and ALS and reduced TAR DNA-binding protein 43 (TDP-43) aggregation in human motor neurons derived from a patient with ALS. Thus, our data suggest that the hydrotropic activity of ATP contributes to the regulation of neuronal homeostasis under both physiological and pathological conditions.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 17","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adq6077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Science AdvancesPub Date : 2025-04-18DOI: 10.1126/sciadv.adt1836
Balaji Olety, Yoshiko Usami, Paul Peters, Yuanfei Wu, Heinrich Göttlinger
{"title":"The ectodomain sheddase ADAM10 restricts HIV-1 propagation and is counteracted by Nef","authors":"Balaji Olety, Yoshiko Usami, Paul Peters, Yuanfei Wu, Heinrich Göttlinger","doi":"10.1126/sciadv.adt1836","DOIUrl":"10.1126/sciadv.adt1836","url":null,"abstract":"<div >HIV-1 Nef enhances virus propagation by down-regulating CD4 and SERINC5. However, recent evidence points to the existence of an additional Nef-sensitive restriction mechanism. We now show that Nef suppresses the aberrant cleavage of HIV-1 gp41 by ADAM10, a virion-associated cellular ectodomain sheddase, and thus increases the amount of HIV-1 envelope glycoprotein (Env) on virions. Additionally, Nef inhibits the shedding of at least some cellular ADAM10 substrates, resulting in their accumulation on HIV-1 virions. Whereas Nef<sup>+</sup> HIV-1 replicated only marginally better in the absence of ADAM10, the propagation of Nef<sup>−</sup> HIV-1 was notably rescued in ADAM10<sup>−</sup> T cell lines. Crucially, Nef<sup>−</sup> HIV-1 also benefited from the absence of ADAM10 in primary CD4<sup>+</sup> T cells. Collectively, our results indicate that ADAM10 negatively affects both laboratory-adapted and primary HIV-1 strains by shedding the ectodomains of viral and cellular transmembrane proteins from virions and that Nef rescues virus replication by counteracting ADAM10.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 16","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adt1836","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pyrite stimulates the growth and sulfur oxidation capacity of anoxygenic phototrophic sulfur bacteria in euxinic environments","authors":"Runjie Li, Xiaolei Liu, Geng Wu, Gaoyuan Li, Jing-Hua Chen, Hongchen Jiang, Hailiang Dong","doi":"10.1126/sciadv.adu7080","DOIUrl":"10.1126/sciadv.adu7080","url":null,"abstract":"<div >Anoxygenic phototrophic sulfur bacteria flourish in contemporary and ancient euxinic environments, driving the biogeochemical cycles of carbon and sulfur. However, it is unclear how these strict anaerobes meet their high demand for iron in iron-depleted environments. Here, we report that pyrite, a widespread and highly stable iron sulfide mineral in anoxic, low-temperature environments, can support the growth and metabolic activity of anoxygenic phototrophic sulfur bacteria by serving as the sole iron source under iron-depleted conditions. Transcriptomic and proteomic analyses revealed that pyrite addition substantially up-regulated genes and protein expression involved in photosynthesis, sulfur metabolism, and biosynthesis of organics. Anoxic microbial oxidation of pyritic sulfur and consequent destabilization of the pyrite structure were postulated to facilitate microbial iron acquisition. These findings advance our understanding of the survival strategies of anaerobes in iron-depleted environments and are important for revealing the previously underappreciated bioavailability of pyritic iron in anoxic environments and anoxic weathering of pyrite.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 16","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adu7080","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Science AdvancesPub Date : 2025-04-18DOI: 10.1126/sciadv.adu1052
James Fong, Hannah K. Doyle, Congli Wang, Alexandra E. Boehm, Sofie R. Herbeck, Vimal Prabhu Pandiyan, Brian P. Schmidt, Pavan Tiruveedhula, John E. Vanston, William S. Tuten, Ramkumar Sabesan, Austin Roorda, Ren Ng
{"title":"Novel color via stimulation of individual photoreceptors at population scale","authors":"James Fong, Hannah K. Doyle, Congli Wang, Alexandra E. Boehm, Sofie R. Herbeck, Vimal Prabhu Pandiyan, Brian P. Schmidt, Pavan Tiruveedhula, John E. Vanston, William S. Tuten, Ramkumar Sabesan, Austin Roorda, Ren Ng","doi":"10.1126/sciadv.adu1052","DOIUrl":"10.1126/sciadv.adu1052","url":null,"abstract":"<div >We introduce a principle, Oz, for displaying color imagery: directly controlling the human eye’s photoreceptor activity via cell-by-cell light delivery. Theoretically, novel colors are possible through bypassing the constraints set by the cone spectral sensitivities and activating M cone cells exclusively. In practice, we confirm a partial expansion of colorspace toward that theoretical ideal. Attempting to activate M cones exclusively is shown to elicit a color beyond the natural human gamut, formally measured with color matching by human subjects. They describe the color as blue-green of unprecedented saturation. Further experiments show that subjects perceive Oz colors in image and video form. The prototype targets laser microdoses to thousands of spectrally classified cones under fixational eye motion. These results are proof-of-principle for programmable control over individual photoreceptors at population scale.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 16","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adu1052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Science AdvancesPub Date : 2025-04-18DOI: 10.1126/sciadv.ads4609
Hamed Karimi, Martin Laasmaa, Margus Pihlak, Marko Vendelin
{"title":"Statistical analysis of fluorescence intensity transients with Bayesian methods","authors":"Hamed Karimi, Martin Laasmaa, Margus Pihlak, Marko Vendelin","doi":"10.1126/sciadv.ads4609","DOIUrl":"10.1126/sciadv.ads4609","url":null,"abstract":"<div >Molecular movement and interactions at the single-molecule level, particularly in live cells, are often studied using fluorescence correlation spectroscopy (FCS). While powerful, FCS has notable drawbacks: It requires high laser intensities and long acquisition times, increasing phototoxicity, and often relies on problematic statistical assumptions in data fitting. We introduce fluorescence intensity trace statistical analysis (FITSA), a Bayesian method that directly analyzes fluorescence intensity traces. FITSA offers faster, more stable convergence than previous approaches and provides robust parameter estimation from far shorter measurements than conventional FCS. Our results demonstrate that FITSA achieves comparable precision to FCS while requiring substantially fewer photons. This advantage becomes even more pronounced when accounting for statistical dependencies in FCS analysis, which are often overlooked but necessary for accurate error estimation. By reducing laser exposure, FITSA minimizes phototoxicity effects, representing a major advancement in the quantitative analysis of molecular processes across fields.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 16","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ads4609","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Science AdvancesPub Date : 2025-04-18DOI: 10.1126/sciadv.adt6432
Phil Huss, Kristopher Kieft, Anthony Meger, Kyle Nishikawa, Karthik Anantharaman, Srivatsan Raman
{"title":"Engineering bacteriophages through deep mining of metagenomic motifs","authors":"Phil Huss, Kristopher Kieft, Anthony Meger, Kyle Nishikawa, Karthik Anantharaman, Srivatsan Raman","doi":"10.1126/sciadv.adt6432","DOIUrl":"10.1126/sciadv.adt6432","url":null,"abstract":"<div >Bacteriophages can adapt to new hosts by altering sequence motifs through recombination or convergent evolution. Where these motifs exist and what fitness advantage they confer remains largely unknown. We report a new method, Metagenomic Sequence Informed Functional Scoring (Meta-SIFT), to find sequence motifs in metagenomic datasets to engineer phage activity. Meta-SIFT uses experimental deep mutational scanning data to create sequence profiles to mine metagenomes for functional motifs invisible to other searches. We experimentally tested ~17,000 Meta-SIFT–derived sequence motifs in the receptor binding protein of the T7 phage. The screen revealed thousands of T7 variants with novel host specificity with motifs sourced from distant families. Position, substitution, and location preferences dictated specificity across a panel of 20 hosts and conditions. To demonstrate therapeutic utility, we engineered active T7 variants against foodborne pathogen <i>Escherichia coli</i> O121. Meta-SIFT is a powerful tool to unlock the potential encoded in phage metagenomes to engineer bacteriophages.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 16","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adt6432","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Science AdvancesPub Date : 2025-04-18DOI: 10.1126/sciadv.ads7903
Nathaniel P. Mohar, Christopher J. Langland, Zachary Darr, Jill Viles, Steven A. Moore, Benjamin W. Darbro, Lori L. Wallrath
{"title":"A genetic variant in SMAD7 acts as a modifier of LMNA-associated muscular dystrophy, implicating SMAD signaling as a therapeutic target","authors":"Nathaniel P. Mohar, Christopher J. Langland, Zachary Darr, Jill Viles, Steven A. Moore, Benjamin W. Darbro, Lori L. Wallrath","doi":"10.1126/sciadv.ads7903","DOIUrl":"10.1126/sciadv.ads7903","url":null,"abstract":"<div >Mutations in <i>LMNA</i> cause multiple types of muscular dystrophy (<i>LMNA</i>-MD). The symptoms of <i>LMNA</i>-MD are highly variable and sensitive to genetic background. To identify genetic contributions to this phenotypic variability, we performed whole-genome sequencing on four siblings possessing the same <i>LMNA</i> mutation with differing degrees of skeletal muscle disease severity. We identified a variant in <i>SMAD7</i> that segregated with severe muscle disease. To functionally test the <i>SMAD7</i> variant, we generated a <i>Drosophila</i> model possessing the <i>LMNA</i> mutation and the <i>SMAD7</i> variant in the orthologous fly genes. The <i>SMAD7</i> variant increased SMAD signaling and enhanced muscle defects caused by the mutant lamin. Conversely, overexpression of wild-type <i>SMAD7</i> rescued muscle function. These findings were extended to humans by showing that SMAD signaling is increased in muscle biopsy tissue from individuals with <i>LMNA</i>-MD compared to age-matched controls. Collectively, our findings support <i>SMAD7</i> as the first functionally tested genetic modifier for <i>LMNA</i>-MD and suggest components of the SMAD pathway as therapeutic targets.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 16","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ads7903","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Science AdvancesPub Date : 2025-04-18DOI: 10.1126/sciadv.adt6113
Emily A. McGlade, Jiude Mao, Kalli K. Stephens, Ryan M. Marquardt, Feyza Nur Arguc, Peter F. Lais, San-Pin Wu, Sarayut Winuthayanon, Haval Shirwan, Esma S. Yolcu, Mark I. Hunter, James K. Pru, John P. Lydon, Francesco J. DeMayo, Wipawee Winuthayanon
{"title":"Progesterone signaling in oviductal epithelial cells modulates the immune response to support preimplantation embryonic development","authors":"Emily A. McGlade, Jiude Mao, Kalli K. Stephens, Ryan M. Marquardt, Feyza Nur Arguc, Peter F. Lais, San-Pin Wu, Sarayut Winuthayanon, Haval Shirwan, Esma S. Yolcu, Mark I. Hunter, James K. Pru, John P. Lydon, Francesco J. DeMayo, Wipawee Winuthayanon","doi":"10.1126/sciadv.adt6113","DOIUrl":"10.1126/sciadv.adt6113","url":null,"abstract":"<div >More than 60% of pregnancy losses occur during the first trimester, highlighting the need to understand the role of the oviduct in early pregnancy. In this study, we conditionally ablated the classical progesterone receptor (<i>Pgr</i>) in oviductal epithelial cells, called the <i>Pgr</i><sup>d/d</sup> mouse model. We found that 40% of embryos collected from <i>Pgr</i><sup>d/d</sup> females were nonviable or developmentally delayed, indicating that epithelial PGR expression is crucial for embryonic development. Single-cell RNA sequencing revealed up-regulation of proinflammatory genes, including interleukin-22 (IL-22), in the epithelial cells of <i>Pgr</i><sup>d/d</sup> females. Pharmacological inhibition of inflammation using nonsteroidal anti-inflammatory drugs significantly reduced IL-22 levels in the oviducts and rescued embryonic developmental rates in <i>Pgr</i><sup>d/d</sup> females. Coculture of wild-type zygotes with IL-22 significantly decreased the number of expanded blastocysts. Our findings suggest that progesterone signaling is vital for immunoregulation and normal preimplantation development, potentially providing insights for developing diagnostic tools and therapeutic strategies to address pregnancy failures.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 16","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adt6113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Science AdvancesPub Date : 2025-04-18DOI: 10.1126/sciadv.ads7933
Tamanash Bhattacharya, Eva M. Alleman, Tiia S. Freeman, Alexander C. Noyola, Michael Emerman, Harmit S. Malik
{"title":"A conserved opal termination codon optimizes a temperature-dependent trade-off between protein production and processing in alphaviruses","authors":"Tamanash Bhattacharya, Eva M. Alleman, Tiia S. Freeman, Alexander C. Noyola, Michael Emerman, Harmit S. Malik","doi":"10.1126/sciadv.ads7933","DOIUrl":"10.1126/sciadv.ads7933","url":null,"abstract":"<div >Most mosquito-transmitted alphaviruses encode a premature opal termination codon upstream of their viral polymerase. We show that the Sindbis virus (SINV) opal codon outperforms other stop codons in primate cells at 37°C due to optimal translational readthrough. However, increased readthrough of all stop codons reduces opal preference at 28°C in primate and mosquito cells. Opal also outperforms all sense codons because opal-to-sense substitutions lead to excess polyprotein production at 37°C, disrupting orderly polyprotein processing and production of viral genomic RNAs (gRNAs) required for virus production. Increased readthrough at 28°C dampens the fitness advantages of opal codons. Unexpectedly, we find that a naturally occurring SINV mutation restores sense-codon fitness by further delaying polyprotein processing, allowing adequate time to produce gRNAs. Similar temperature-dependent mechanisms occur in the distantly related dual-host alphavirus, Ross River virus. Our work highlights sophisticated strategies dual-host alphaviruses use to optimize replication in divergent temperatures through a single codon.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 16","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ads7933","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Science AdvancesPub Date : 2025-04-18DOI: 10.1126/sciadv.ads8733
Abdullah H. Bukhamsin, Saptami S. Shetty, Esraa Fakeih, Mario Soto Martinez, Cecilia Lerma, Mufeeda Mundummal, Jian You Wang, Jürgen Kosel, Salim Al-Babili, Ikram Blilou, Khaled N. Salama
{"title":"In vivo dynamics of indole- and phenol-derived plant hormones: Long-term, continuous, and minimally invasive phytohormone sensor","authors":"Abdullah H. Bukhamsin, Saptami S. Shetty, Esraa Fakeih, Mario Soto Martinez, Cecilia Lerma, Mufeeda Mundummal, Jian You Wang, Jürgen Kosel, Salim Al-Babili, Ikram Blilou, Khaled N. Salama","doi":"10.1126/sciadv.ads8733","DOIUrl":"10.1126/sciadv.ads8733","url":null,"abstract":"<div >Specific phytohormone combinations regulate plant growth and responses to environmental stimuli. Monitoring their distribution is key for understanding signaling cross-talk and detecting plant stress early. However, typical means of monitoring these chemicals are often laborious, destructive, or limited to model plants. In this study, we present an amperometric and minimally invasive sensing platform that can be attached to plant leaves for the simultaneous detection of two key phytohormones, auxin [indole-3-acetic acid (IAA)] and salicylic acid (SA). The platform incorporates magnetized microneedles coated with superparamagnetic Fe<sub>3</sub>O<sub>4</sub> intercalated into a scaffold of multiwalled carbon nanotubes (MWCNTs). It achieves detection limits of 1.41 μM (IAA) and 1.15 μM (SA) with a strong correlation (<i>R</i><sup>2</sup> ≥ 0.7) to ultrahigh-performance liquid chromatography–tandem mass spectrometry measurements. Furthermore, implementing cyclical amperometric cleaning extends the sensor lifespan by preventing electrode passivation. Last, the sensor’s capability to monitor the real-time plant responses to several stressors is validated, showcasing its potential for phytodiagnostics and precision farming.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 16","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ads8733","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}