Molecular Nutrition & Food Research最新文献_第3页

Effects of Dietary Fiber and Acetate on Alcoholic Heart Disease and Intestinal Microbes in Mice 膳食纤维和醋酸酯对小鼠酒精性心脏病和肠道微生物的影响

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-18 DOI: 10.1002/mnfr.70040

Wei Siang, Jiang Li Jin, Jiao Yinming, Lin Wenji, Feng Yan

{"title":"Effects of Dietary Fiber and Acetate on Alcoholic Heart Disease and Intestinal Microbes in Mice","authors":"Wei Siang, Jiang Li Jin, Jiao Yinming, Lin Wenji, Feng Yan","doi":"10.1002/mnfr.70040","DOIUrl":"https://doi.org/10.1002/mnfr.70040","url":null,"abstract":"Alcoholic heart disease (AHD) is a severe cardiovascular condition linked to chronic alcohol consumption. This study investigates the effects of a high-fiber diet and acetate on gut microbiota and cardiac function in AHD mouse models. Sixty male C57BL/6 mice were divided into six groups, receiving either a control diet, high-fiber diet, or acetate supplementation alongside alcohol treatment. Results revealed that cardiac fibrosis and heart failure were notably improved in the AHD mice receiving high-fiber or acetate diets. Transcriptomic analyses indicated that dietary interventions modulated the expression of genes involved in lipid metabolism and the TGF-β signaling pathway. Additionally, 16S rRNA sequencing showed that the high-fiber diet and acetate altered gut microbiota composition, enhancing the abundance of beneficial bacteria such as Akkermansia muciniphila, Lactobacillus intestinalis, and Bacteroides acidifaciens. These microbes exhibited positive correlations with genes related to fat metabolism and TGF-β signaling, suggesting a potential mechanism for gut microbiota's role in AHD pathology. ROC analysis identified these bacteria as promising biomarkers for AHD detection. Overall, our findings underscore the therapeutic potential of dietary fiber and acetate in modulating gut microbiota and improving cardiac function in AHD, highlighting the intricate relationship between gut health and cardiovascular disease management.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"37 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the Fatty Acid Intake on Cognitive Function Through Gut Microbiota and Circulating microRNAs Remodeling in Middle-Aged and Elderly Overweight and Obese Population 脂肪酸摄入通过肠道菌群和循环microrna重塑对中老年超重和肥胖人群认知功能的影响

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-18 DOI: 10.1002/mnfr.70027

Jinchen Li, Mengdi Lu, Hongying Huang, Tong Zhao, Huiyan Yu, Cui Zhou, Weiwei Ma

{"title":"Effect of the Fatty Acid Intake on Cognitive Function Through Gut Microbiota and Circulating microRNAs Remodeling in Middle-Aged and Elderly Overweight and Obese Population","authors":"Jinchen Li, Mengdi Lu, Hongying Huang, Tong Zhao, Huiyan Yu, Cui Zhou, Weiwei Ma","doi":"10.1002/mnfr.70027","DOIUrl":"https://doi.org/10.1002/mnfr.70027","url":null,"abstract":"This study aimed to explore the role of fatty acids in regulating cognitive function through the gut microbiota (GM) and circulating microRNAs (miRNAs) by comprehensive analysis of dietary fatty acid intake (erythrocyte membrane fatty acid composition) and the GM and miRNAs in people with different body mass index (BMI). Participants with different BMIs in Beijing (normal weight [NW], overweight [OW], and obese [OB]) were recruited according to the Chinese obesity diagnostic criteria. Compared with the NW group, cognitive decline was observed in the OW and OB groups. Dietary saturated fatty acid (SFA) intake increased and dietary unsaturated fatty acid intake decreased in NW people. In the OW group, C18:3n-3 had beneficial mediation effects for cognition. Conversely, C18:3n-6 showed adverse cognitive effects in the mediating analysis. In the OW group, hsa-miR-142-5p presented a negative correlation with cognitive function. Erythrocyte membrane C23:0, as a good indicator of dietary fat intake, was found to influence cognitive function through Fusobacteriota, Proteobacteria, and plasma hsa-miR-144-3p in OB individuals. Different types of dietary fatty acid intake may affect the interaction between GM and miRNAs, and exert cognitive effects on middle-aged and elderly population, forming an interactive network of fatty acids-GM/miRNAs-cognition.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"43 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus Coagulans BC99 Protects Ionizing Radiation-Induced Intestinal Injury and Modulates Gut Microbiota and Metabolites in Mice 凝固芽孢杆菌BC99保护电离辐射引起的小鼠肠道损伤并调节肠道微生物群和代谢物

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-17 DOI: 10.1002/mnfr.70057

Lin-Feng Tang, Feng-Ling Tang, Hao Zhou, Ze-Kun Li, Chao-Qun Pi, Yang He, Ming Li

{"title":"Bacillus Coagulans BC99 Protects Ionizing Radiation-Induced Intestinal Injury and Modulates Gut Microbiota and Metabolites in Mice","authors":"Lin-Feng Tang, Feng-Ling Tang, Hao Zhou, Ze-Kun Li, Chao-Qun Pi, Yang He, Ming Li","doi":"10.1002/mnfr.70057","DOIUrl":"https://doi.org/10.1002/mnfr.70057","url":null,"abstract":"The gastrointestinal tract is highly sensitive to ionizing radiation (IR), which causes radiation-induced intestinal injury (RIII). There are no effective drugs available for RIII in routine clinical treatment, which is a major limiting factor during the process of radiotherapy for pelvic abdominal malignancies. In this study, we aimed to elucidate the potential of probiotic Bacillus coagulans BC99 (B.coagulans BC99) in preventing RIII. C57BL/6J mice were gavage-administered with B.coagulans BC99 for 30 days and then exposed to a single dose of 12 Gy x-ray whole abdominal irradiation (WAI). B.coagulans BC99 treatment could mitigate RIII by preventing weight loss, maintaining the integrity of intestinal structure and barrier, improving inflammatory symptoms, modulating oxidative stress, and regulating the composition of gut microbiota, thereby reestablishing intestinal homeostasis. In addition, the potential radioprotective mechanism of B.coagulans BC99 was closely related to the gut microbiota-derived metabolites. This study offers a novel perspective for advancing probiotic-based treatments for RIII and enhancing strategies for the prevention of RIII.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"108 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Insights Into Naringenin: A Multifaceted Exploration of Production, Synthesis, Health Effects, Nanodelivery Systems, and Molecular Simulation 柚皮素的新见解：生产、合成、健康影响、纳米给药系统和分子模拟的多方面探索

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-14 DOI: 10.1002/mnfr.70066

Yuanqiang Jia, Xinjing Zhou, Yanan Liu, Xiaoyong Liu, Feiyue Ren, Hongzhi Liu

{"title":"Novel Insights Into Naringenin: A Multifaceted Exploration of Production, Synthesis, Health Effects, Nanodelivery Systems, and Molecular Simulation","authors":"Yuanqiang Jia, Xinjing Zhou, Yanan Liu, Xiaoyong Liu, Feiyue Ren, Hongzhi Liu","doi":"10.1002/mnfr.70066","DOIUrl":"https://doi.org/10.1002/mnfr.70066","url":null,"abstract":"Naringenin, a flavonoid widely present in citrus fruits, has garnered considerable attention due to its diverse biological activities and health-promoting benefits. As research on naringenin advances, the application scope of naringenin has significantly expanded. This paper provides a systematic overview of the production and synthesis methods of naringenin, focusing especially on the application of green extraction techniques and the strategies for constructing microbial metabolic engineering. Naringenin not only achieves its diverse biological activities including antioxidant, antiinflammatory, and glucolipid metabolism regulation through multiple mechanisms but also modulates the balance of gut microbiota, thereby mediating synergistic health effects via the host–microbial metabolic axis. Given the low oral bioavailability of naringenin, various nanodelivery systems have been developed to improve its bioavailability. Meanwhile, molecular simulation techniques elucidate the binding conformation characteristics with receptors at the molecular level, providing novel insights into its mechanisms of action. In conclusion, this review seeks to offer a theoretical basis and future directions for further research and application of naringenin.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"6 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma Metabolite Profiles Linked to EAT-Lancet Diet and Risk of Inflammatory Bowel Disease: A Prospective Cohort Study From UK Biobank 血浆代谢物谱与柳叶刀饮食和炎症性肠病风险相关：来自英国生物银行的前瞻性队列研究

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-13 DOI: 10.1002/mnfr.70059

Xiaohua Ye, Chunmin Lou, Zhe Shen

{"title":"Plasma Metabolite Profiles Linked to EAT-Lancet Diet and Risk of Inflammatory Bowel Disease: A Prospective Cohort Study From UK Biobank","authors":"Xiaohua Ye, Chunmin Lou, Zhe Shen","doi":"10.1002/mnfr.70059","DOIUrl":"https://doi.org/10.1002/mnfr.70059","url":null,"abstract":"The EAT-Lancet Commission recommends a sustainable, health-focused diet, but its impact on inflammatory bowel disease (IBD) and metabolic mechanisms remains unclear. This study explores the association between the EAT-Lancet diet and IBD risk. We included 187 558 participants from the UK Biobank, free from IBD at baseline. Dietary intake was assessed using a validated 24-h dietary recall, and an EAT-Lancet diet score was calculated. Incident IBD cases were identified through primary care, hospital data, and death registries. Cox proportional hazards models were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs). Over a mean follow-up of 13.15 years, 571 cases of Crohn's disease (CD) and 1066 of ulcerative colitis (UC) were recorded. HRs per 1-point increase in the EAT-Lancet score were 0.95 (95% CI: 0.91–0.98) for IBD, 0.95 (95% CI: 0.91–1.00) for UC, and 0.93 (95% CI: 0.87–0.98) for CD. Seven metabolites were identified as mediators, accounting for 10.7% of the association. Greater adherence to the EAT-Lancet diet was associated with a trend toward reduced IBD risk, with identified metabolites mediating this association. These findings emphasize the nutritional relevance of the EAT-Lancet diet in reducing IBD risk.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"112 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenols From Mallotus peltatus (Geiseler) Mull. Arg. Ameliorates FFA-Induced Hepatic Steatosis in L02 Cells and Reduces Lipid Accumulation in Caenorhabditis elegans 马蹄莲多酚。参数。改善ffa诱导的L02细胞肝脂肪变性，减少秀丽隐杆线虫的脂质积累

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-10 DOI: 10.1002/mnfr.202400689

Ke-Xin Hao, Ying-Jing Zhang, Yi-Meng Li, Rui-Fang Zhong, Ling Wang, Xiao Chang, Jian-Guo Jiang, Wei Zhu

{"title":"Polyphenols From Mallotus peltatus (Geiseler) Mull. Arg. Ameliorates FFA-Induced Hepatic Steatosis in L02 Cells and Reduces Lipid Accumulation in Caenorhabditis elegans","authors":"Ke-Xin Hao, Ying-Jing Zhang, Yi-Meng Li, Rui-Fang Zhong, Ling Wang, Xiao Chang, Jian-Guo Jiang, Wei Zhu","doi":"10.1002/mnfr.202400689","DOIUrl":"https://doi.org/10.1002/mnfr.202400689","url":null,"abstract":"Mallotus peltatus (Geiseler) Mull. Arg. (MPMA) is a specialty plant used to make tea in Hainan Province, China. However, its hypolipidemic activity has been rarely studied. In this study, three polyphenol fractions (MPMAP-1, MPMAP-2, and MPMAP-3) were purified from a 60% ethanol extract of MPMA, and the hypolipidemic activities were evaluated by establishing an FFA-induced L02 cell model to determine lipid accumulation, antioxidant enzyme activities, and gene levels related to the Nrf2/ARE pathway and lipid metabolism. In addition, noninduced and high glucose-induced models were established using Caenorhabditis elegans (C. elegans) to evaluate the lipid-lowering activity of MPMAP-1. The results showed that all three polyphenols could significantly inhibit lipid accumulation, reduce intracellular MDA content, and enhance the activities of CAT, SOD, and GPx in FFA-induced L02 cells. The qRT-PCR results indicated that the amount of fat accumulation in L02 cells could be regulated by modulating the relative expression of mRNA in the Nrf2/ARE signaling pathway and lipid metabolism pathway. The noninduced model and high glucose-induced model demonstrated that MPMAP-1 was able to reduce lipid accumulation and ROS levels and increase the activities of antioxidant enzymes in C. elegans. In summary, our results suggested that polyphenol compounds of MPMA may be a promising natural product for lipid-lowering.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"100 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143814193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Antimicrobial Potential of Lactiplantibacillus plantarum and Their Derived Extracellular Vesicles 植物乳杆菌及其衍生细胞外囊泡的抑菌潜力评价

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-10 DOI: 10.1002/mnfr.70035

Pooja Yadav, Nabendu Debnath, Praveen Kumar Mehta, Ashwani Kumar, Ashok Kumar Yadav

引用次数: 0

Effects of Natural Polysaccharides on the Gut Microbiota Related to Human Metabolic Health 天然多糖对人体代谢健康相关肠道菌群的影响

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-10 DOI: 10.1002/mnfr.202400792

Yigang Huang, Hongfei Guo, Yuchen Liu, Wengang Jin, Chella Perumal Palanisamy, Jinjin Pei, Fatih Oz, A. M. Abd El-Aty

{"title":"Effects of Natural Polysaccharides on the Gut Microbiota Related to Human Metabolic Health","authors":"Yigang Huang, Hongfei Guo, Yuchen Liu, Wengang Jin, Chella Perumal Palanisamy, Jinjin Pei, Fatih Oz, A. M. Abd El-Aty","doi":"10.1002/mnfr.202400792","DOIUrl":"https://doi.org/10.1002/mnfr.202400792","url":null,"abstract":"Natural polysaccharides (NPs) are sugar chains bound by glycosidic bonds that are composed of at least 10 monosaccharides and have broad biological activity. The human body microbiome is a complex ecosystem that plays a role in host metabolism, immunity, and other important life activities. Numerous studies have demonstrated an obvious relationship between the gut flora and the occurrence of many human diseases. Many studies have reviewed and investigated the effects of polysaccharides on the microbiome, but the underlying mechanisms remain unclear. Most of these studies have focused on the effects of NPs on microbes, as they are important “foods” for the intestinal flora. However, polysaccharides can also affect microbes by improving gut homeostasis. Therefore, the purpose of this review is to introduce recent research that looks at how NPs affect microbiomes by directly acting as fermentation substrates and enhancing gut homeostasis. In addition, this study provides a succinct summary of NP extraction, purification, and structural characteristics, as well as a discussion of their structure‒activity correlations. This study also sheds light on future directions and obstacles in the use of NPs with protective properties, with the aim of providing insights into their potential applications in disease treatment.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"101 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143814195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the Nutraceutical Properties of Wild Strawberry (Fragaria vesca L.) Extracts on Human Colorectal Cell Lines 野生草莓（Fragaria vesca L.）营养保健特性的评价人结肠直肠癌细胞系提取物

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-10 DOI: 10.1002/mnfr.70018

Monica Oldani, Lorenzo Guzzetti, Emiliano Pioltelli, Davide Sala, Davide Panzeri, Maura Brioschi, Matilde Forcella, Paola Fusi

{"title":"Assessment of the Nutraceutical Properties of Wild Strawberry (Fragaria vesca L.) Extracts on Human Colorectal Cell Lines","authors":"Monica Oldani, Lorenzo Guzzetti, Emiliano Pioltelli, Davide Sala, Davide Panzeri, Maura Brioschi, Matilde Forcella, Paola Fusi","doi":"10.1002/mnfr.70018","DOIUrl":"https://doi.org/10.1002/mnfr.70018","url":null,"abstract":"Colorectal cancer is the third most common cancer worldwide; its higher incidence in the Western world, compared to rural areas of Africa and Asia, led to its classification among the so-called Westernized diseases. The wild strawberry Fragaria vesca L. is endowed with several bioactive components, such as polyphenols, vitamins, terpenes, and organic acids that can contribute to cancer prevention. In this study, we chemically characterized a wild strawberry extract through high-resolution mass spectrometry and evaluated its antioxidant properties on two human colorectal cancer cell lines: KRAS mutated SW480 cells and E705 cells. We found that treatment with the extract induced cell cycle arrest in the G2 phase in SW480 cells, while it led E705 cells to apoptosis through a significant increase in the reactive oxygen species level. Wild strawberry extract is a promising dietary supplement for both wild-type and KRAS-mutated patients who exhibit a more aggressive cancer phenotype. In addition, the lack of toxicity of wild strawberry extract toward healthy colorectal cells makes this food a promising chemopreventive nutritional supplement.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"23 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143814192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Milk Exosomes From Gestational Diabetes Mellitus Parturients Demonstrate Weaker Ability to Promote Intestinal Development in Offspring 妊娠期糖尿病患者乳外泌体促进后代肠道发育的能力较弱

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-04-10 DOI: 10.1002/mnfr.70026

Jiaqi Mo, Yudi Ding, Junyi Yang, Zhongdaixi Zheng, Jiazhi Lu, Huiyu Luo, Jiexian Wang, Fengjuan Lin, Junbin Chen, Qing Li, Xiangyi Zheng, Longying Zha

{"title":"Milk Exosomes From Gestational Diabetes Mellitus Parturients Demonstrate Weaker Ability to Promote Intestinal Development in Offspring","authors":"Jiaqi Mo, Yudi Ding, Junyi Yang, Zhongdaixi Zheng, Jiazhi Lu, Huiyu Luo, Jiexian Wang, Fengjuan Lin, Junbin Chen, Qing Li, Xiangyi Zheng, Longying Zha","doi":"10.1002/mnfr.70026","DOIUrl":"https://doi.org/10.1002/mnfr.70026","url":null,"abstract":"This study aims to investigate whether human milk exosomes from gestational diabetes mellitus (GDM-EXO) and healthy (HEA-EXO) parturients differ in regulating intestinal development in offspring. The differential miRNAs associated with intestinal development in GDM-EXO and HEA-EXO were verified by using qPCR and their relationships with gut microbiota (GM) in infants were analyzed. C57BL/6J mice were gavaged with 50 mg/kg·BW HEA-EXO or GDM-EXO. The intestinal morphology, gut barriers, ZO-1 and Occludin, and GM were determined by histological staining, Western blotting, and 16S rDNA amplicon sequencing, respectively. Hsa-miR-19b-3p, hsa-miR-148a-3p, and hsa-miR-320a-3p were upregulated, and hsa-miR-429 was decreased in GDM-EXO compared to HEA-EXO. The GDM parturients’ infants had increased intestinal Coriobacteriaceae, Clostridiaceae, Erysipelotrichaceae, Erysipelatoclostridiaceae, and fewer Lactobacillaceae than the healthy parturient's infants. The four differential miRNAs in GDM-EXO all correlated with the infants’ GM. GDM-EXO- and HEA-EXO-fed mice had greater villus lengths, villus length-to-crypt depth ratios, goblet cell numbers, elevated ZO-1 and Occludin, and lower crypt depths than control mice. HEA-EXO-fed mice had better intestinal morphology and gut barrier integrity than GDM-EXO-fed mice. GDM-EXO-fed mice had significantly decreased Lachnospiraceae and Oscillospiraceae than HEA-EXO-fed mice. GDM-EXO demonstrate weaker ability to promote intestinal development in offspring than HEA-EXO.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"25 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143814194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0