Revista Iberoamericana De Micologia最新文献

{"title":"Cyberlindnera fabianii: primer aislamiento clínico en Chile","authors":"Eduardo Álvarez Duarte ,&nbsp;Nicolás Cepeda","doi":"10.1016/j.riam.2021.04.009","DOIUrl":"https://doi.org/10.1016/j.riam.2021.04.009","url":null,"abstract":"<div><h3>Background</h3><p>Although considered an unusual etiological agent, <em>Cyberlindnera</em> <em>(Candida)</em> <em>fabianii</em> has been related to septicemia in several reports in recent years. Its doubtful or uncertain identification when using tests such as CHROMagar Candida, API® Candida, API® ID32C or VITEK® MS, leads to an underestimation of the cases produced by this yeast.</p></div><div><h3>Aims</h3><p>To report the first isolation of <em>C</em>. <em>fabianii</em> in Chile and its identification.</p></div><div><h3>Methods</h3><p>The sequencing of the internal transcribed spacer region (ITS) was performed. Antifungal susceptibility profiles were obtained by means of the broth microdilution technique.</p></div><div><h3>Results</h3><p>The identification was only reached by sequencing the ITS regions, which shows the limited usefulness of the conventional techniques in the identification of some yeast species. A dendrogram shows the phylogenetic relationship of the isolated strain with some other yeast species.</p></div><div><h3>Conclusion</h3><p>In the identification of fastidious microorganisms or microorganisms whose identification is not completely reliable when using classical or even advanced methodologies, such as mass spectrometry, sequencing techniques are essential.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 180-183"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2021.04.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90023490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, enzymatic activity and biofilm formation of Candida species isolated from goat milk","authors":"Carolina Segundo Zaragoza ,&nbsp;Itzel López Ortiz ,&nbsp;David Alejandro Contreras Caro del Castillo ,&nbsp;Yesmín María Domínguez Hernández ,&nbsp;Juan Antonio Rodríguez García","doi":"10.1016/j.riam.2021.06.001","DOIUrl":"10.1016/j.riam.2021.06.001","url":null,"abstract":"<div><h3>Background</h3><p>Data regarding yeast microbiota in goat milk is scarce.</p></div><div><h3>Aims</h3><p>To isolate and identify species of the genus <em>Candida</em> in milk samples from clinically healthy goats, and evaluate their enzymatic activity and biofilm formation.</p></div><div><h3>Methods</h3><p>1092 milk samples from clinically healthy goats were collected and processed. The yeast isolates were identified by phenotypic, methods and their enzymatic activity (phospholipase, hemolysin and protease) and biofilm formation evaluated.</p></div><div><h3>Results</h3><p>We obtained 221 <em>Candida</em> isolates belonging to six species: <em>Candida kefyr</em> (35.7%), <em>Candida guilliermondii</em> (33%), <em>Candida famata</em> (23.5%), <em>Candida glabrata</em> (5.9%), <em>Candida albicans</em> (1.35%) and <em>Candida parapsilosis</em> <em>sensu lato</em> (0.45%). Protease activity was detected in all <em>Candida</em> species while hemolysin activity was only present in <em>C. kefyr, C. guilliermondii</em>, <em>C. famata</em> and <em>C. albicans</em>. Only <em>C. albicans</em> showed phospholipase activity. With the exception of <em>C. parapsilosis sensu lato</em>, all <em>Candida</em> species formed biofilm, with 60.19% of the isolates being poor producers, 9.93% moderate producers, and 1.35% strong producers.</p></div><div><h3>Conclusions</h3><p>The milk of clinically healthy goats contains several species of the genus <em>Candida</em> that could play a role as opportunistic pathogens in mastitis.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 175-179"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2021.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39407771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Super moulds and Scedosporium species","authors":"F. Javier Cabañes","doi":"10.1016/j.riam.2021.05.001","DOIUrl":"10.1016/j.riam.2021.05.001","url":null,"abstract":"","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 157-158"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2021.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39172862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentially expressed proteins in the interaction of Paracoccidioides lutzii with human monocytes","authors":"Flamélia Carla Silva Oliveira ,&nbsp;Wallace Felipe Blohem Pessoa ,&nbsp;Joise Hander Mares ,&nbsp;Herbert Pina Silva Freire ,&nbsp;Ednara Almeida de Souza ,&nbsp;Carlos Priminho Pirovani ,&nbsp;Carla Cristina Romano","doi":"10.1016/j.riam.2020.09.006","DOIUrl":"10.1016/j.riam.2020.09.006","url":null,"abstract":"<div><h3>Background</h3><p>Fungi of the genus <span><em>Paracoccidioides</em></span> are the etiological agents of paracoccidioidomycosis, a highly prevalent mycosis in Latin America. Infection in humans occurs by the inhalation of conidia, which later revert to the form of yeast. In this context, macrophages are positioned as an important line of defense, assisting in the recognition and presentation of antigens, as well as producing reactive oxygen species that inhibit fungal spreading.</p></div><div><h3>Aims</h3><p>The objective of this study was to identify differentially expressed proteins during the interaction between <em>Paracoccidioides lutzii</em> Pb01 strain and human U937 monocytes.</p></div><div><h3>Methods</h3><p>Two-dimensional electrophoresis, combined with mass spectrometry, was used to evaluate the differential proteomic profiles of the fungus <em>P. lutzii</em> (Pb01) interacting with U937 monocytes.</p></div><div><h3>Results</h3><p>It was possible to identify 25 proteins differentially expressed by Pb01 alone and after interacting with U937 monocytes. Most of these proteins are directly associated with fungal metabolism for energy generation, such as glyceraldehyde-3-phosphate dehydrogenase, and intracellular adaptation to monocytes. Antioxidant proteins involved in the response to oxidative stress, such as peroxiredoxin, cytochrome, and peroxidase, were expressed in greater quantity in the interaction with monocytes, suggesting their association with survival mechanisms inside phagocytic cells. We also identified 12 proteins differentially expressed in monocytes before and after the interaction with the fungus; proteins involved in the reorganization of the cytoskeleton, such as vimentin, and proteins involved in the response to oxidative stress, such as glioxalase 1, were identified.</p></div><div><h3>Conclusions</h3><p>The results of this proteomic study of a <em>P. lutzii</em> isolate are novel, mimicking in vitro what occurs in human infections. In addition, the proteins identified may aid to understand fungal–monocyte interactions and the pathogenesis of paracoccidioidomycosis.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 159-167"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39642633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anidulafungin versus fluconazole in the treatment of Candida albicans chorioretinitis","authors":"Celia Ruiz-Arranz ,&nbsp;Eugenio Pérez-Blázquez ,&nbsp;Almudena De Pablo-Cabrera ,&nbsp;Manuel Ferro-Osuna","doi":"10.1016/j.riam.2021.05.002","DOIUrl":"10.1016/j.riam.2021.05.002","url":null,"abstract":"<div><h3>Background</h3><p><span><em>Candida albicans</em></span> chorioretinitis is the most common cause of endogenous fungal endophthalmitis. Echinocandins are recommended as first-line therapy in the treatment of invasive candidiasis (IC), but in clinically stable patients with IC and endophthalmitis caused by <em>Candida</em> species susceptible to azole compounds these are the first-line treatment due to their better intraocular penetration.</p></div><div><h3>Case <em>report</em></h3><p>A 42-year-old woman admitted to hospital for duodenal perforation after gastrointestinal surgery and treated with broad-spectrum antibiotics developed <em>C. albicans</em><span> candidemia<span>. According to protocol, an antifungal treatment with anidulafungin was given. The patient presented no visual symptoms but on routinary ophthalmoscopic examination multiple bilateral chorioretinal lesions were observed. Systemic therapy was changed to fluconazole, with good systemic and ocular results.</span></span></p></div><div><h3>Conclusions</h3><p>Azole compounds are the first-line therapy for endophthalmitis associated with candidemia. However, clinical guidelines often propose echinocandins as the first option for IC. In some cases, <em>C. albicans</em> chorioretinitis will require a change in the systemic treatment to assure better intraocular penetration. According to the current evidence and our own experience, routine funduscopy is not necessary in all IC patients. However, we do recommend fundus examination in patients with visual symptoms or those unable to report them (paediatric patients and patients with an altered level of consciousness), and in those who are being treated with echinocandins in monotherapy.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 188-191"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39718160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kerion Celsi caused by Trichophyton tonsurans with dermatophytid reaction","authors":"Elisabeth Gómez-Moyano ,&nbsp;Ana María Fernández-Sánchez ,&nbsp;Vicente Crespo-Erchiga ,&nbsp;Leandro Martínez-Pilar","doi":"10.1016/j.riam.2020.12.002","DOIUrl":"10.1016/j.riam.2020.12.002","url":null,"abstract":"","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 3","pages":"Pages 151-152"},"PeriodicalIF":1.9,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.12.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25387954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nosemosis and the collapse of beehives","authors":"F. Javier Cabañes","doi":"10.1016/j.riam.2020.03.003","DOIUrl":"10.1016/j.riam.2020.03.003","url":null,"abstract":"","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 3","pages":"Pages 107-108"},"PeriodicalIF":1.9,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.03.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38077108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal activity of amniotic fluid against different Candida species","authors":"Sofia Maraki ,&nbsp;Dimitra Stafylaki ,&nbsp;Spyridon A. Karageorgos ,&nbsp;Filippos Koutroumpakis ,&nbsp;George Hamilos ,&nbsp;Antonis Makrigiannakis ,&nbsp;George Samonis","doi":"10.1016/j.riam.2021.02.002","DOIUrl":"10.1016/j.riam.2021.02.002","url":null,"abstract":"<div><h3>Background</h3><p>Although <em>Candida</em> is a commensal of the urogenital tract, intrauterine fungal infections are extremely uncommon in clinical practice.</p></div><div><h3>Aims</h3><p>In the present work we evaluated whether amniotic fluid (AF) possesses direct antifungal activity against clinical isolates of <em>Candida</em> <em>albicans</em> and other <em>Candida</em> species.</p></div><div><h3>Methods</h3><p>A total of 23 AF samples from pregnant women with gestational age of 38–41 weeks were obtained under aseptic conditions by the aspiration of the amniotic sac during cesarean section. Different <em>Candida</em> species were inoculated in amniotic fluid and Sabouraud broth, used as control, and were incubated at 37<!--> <!-->°C for 48<!--> <!-->h. Quantitative cultures of test samples and controls were performed at 0, 4, 8, 12, 24, and 48<!--> <!-->h.</p></div><div><h3>Results</h3><p>AF collected from 23 pregnant women had consistent and significant inhibitory activity against all <em>Candida</em> isolates tested. Nonetheless, a complete inhibition of growth by all 23 AF samples tested was observed only against <span><em>Candida glabrata</em></span>.</p></div><div><h3>Conclusions</h3><p>It is likely that the antifungal activity of the AF against <em>C. albicans</em>, <em>C. glabrata</em> and <span><em>Candida parapsilosis</em></span> observed <em>in vitro</em> also exists <em>in vivo</em>, contributing to protect against intrauterine fungal infections.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 3","pages":"Pages 148-150"},"PeriodicalIF":1.9,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2021.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39003340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic recurrent vulvovaginitis is not only due to Candida","authors":"Alicia Arechavala ,&nbsp;Ricardo Negroni ,&nbsp;Gabriela Santiso ,&nbsp;Roxana Depardo ,&nbsp;Pablo Bonvehí","doi":"10.1016/j.riam.2021.03.002","DOIUrl":"10.1016/j.riam.2021.03.002","url":null,"abstract":"<div><h3>Background</h3><p><span>Recurrent vulvovaginitis is a growing problem that affects millions of women worldwide. In many cases it is treated as vulvovaginal </span>candidiasis, but there is not always microbiological confirmation.</p></div><div><h3>Aims</h3><p>To determine the etiology of vulvovaginitis in a group of patients.</p></div><div><h3>Methods</h3><p>This is a cross-sectional study in which the data from the medical records of 316 adult patients who consulted for vulvovaginitis were analyzed. Eighty nine percent of the cases had already suffered previous episodes.</p></div><div><h3>Results</h3><p>The median age was 34 (265 patients were between 16 and 45 years old). Yeasts were isolated in culture from 211 (66.8%) patients, although pseudo-hyphae and yeasts were observed in only 166 samples (52.5%) in the direct microscopic examination. Multiple predisposing factors were found, among which the use of contraceptives or previous antibiotics stand out. Most of the patients (almost 90%) had been treated with antifungals, with or without microbiological confirmation. <span><em>Candida albicans</em></span> was isolated in 187 (88.6%) patients, followed by <span><em>Candida glabrata</em></span><span><span> in 6 (2.8%) patients. Association with bacterial vaginosis was found in 35.1% and with intermediate bacterial </span>microbiota in 33.2% of the cases. A remarkably high proportion of </span><em>C. albicans</em> isolates resistant to fluconazole (80.1%) and itraconazole (58.8%) was found.</p></div><div><h3>Conclusions</h3><p>A microbiological analysis is essential to confirm the diagnosis of vulvovaginal candidiasis, whether simple, complicated, or recurrent. Identifying the isolated yeast species and determining its susceptibility to antifungal agents are particularly important.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 3","pages":"Pages 132-137"},"PeriodicalIF":1.9,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2021.03.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38997786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of pulmonary mycoses in smear-negative patients with suspected tuberculosis in the Brazilian Amazon","authors":"Joycenea da Silva Matsuda ,&nbsp;Bodo Wanke ,&nbsp;Antonio Alcirley da Silva Balieiro ,&nbsp;Carla Silvana da Silva Santos ,&nbsp;Regia Cristina dos Santos Cavalcante ,&nbsp;Mauro de Medeiros Muniz ,&nbsp;Daiana Rodrigues Torres ,&nbsp;Silviane Bezerra Pinheiro ,&nbsp;Hagen Frickmann ,&nbsp;João Vicente Braga Souza ,&nbsp;Flor Ernestina Martinez-Espinosa","doi":"10.1016/j.riam.2020.12.004","DOIUrl":"10.1016/j.riam.2020.12.004","url":null,"abstract":"<div><h3>Background</h3><p>Pulmonary mycoses resemble clinically and radiologically chronic pulmonary tuberculosis. Studies describing the prevalence, etiology and clinical features of pulmonary mycosis are of crucial importance in the Brazilian Amazon.</p></div><div><h3>Aims</h3><p>To estimate the frequency of pulmonary mycoses in smear-negative tuberculosis patients; to describe their demographic, epidemiological, and clinical characteristics; and to evaluate diagnostic methods.</p></div><div><h3>Methods</h3><p>A cross-sectional study was conducted at two tuberculosis reference institutions in Amazonas, Brazil. We included 213 patients and collected clinical data, blood and induced sputum to perform serological, direct microscopy, microbiologic culture and PCR-based assays to identify infections caused by <span><em>Aspergillus fumigatus</em></span>, <span><em>Paracoccidioides brasiliensis</em></span>, <span><em>Histoplasma capsulatum</em></span>, <span><em>Cryptococcus</em></span>, and HIV. Chest computed tomography was also performed.</p></div><div><h3>Results</h3><p>Pulmonary mycoses were diagnosed in 7% (15/213) of the cases, comprising ten aspergillosis cases, three cases of paracoccidioidomycosis and one case each of histoplasmosis and cryptococcosis. Among the patients with pulmonary mycoses, 86.7% were former tuberculosis patients. The most significant clinical characteristics associated with pulmonary mycoses were cavity-shaped lung injuries, prolonged chronic cough and hemoptysis.</p></div><div><h3>Conclusions</h3><p>Our study confirmed the high prevalence of pulmonary mycoses in smear-negative tuberculosis patients in the Brazilian Amazon.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 3","pages":"Pages 111-118"},"PeriodicalIF":1.9,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.12.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25523218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}