Revista Iberoamericana De Micologia最新文献

{"title":"Pneumocystis jirovecii in the lower respiratory tract of immunocompetent individuals.","authors":"Beatriz Gálvez, Consuelo Ferrer, Violeta Esteban, José Noberto Sancho-Chust, Beatriz Amat, Eusebi Chiner, Maria Francisca Colom","doi":"10.1016/j.riam.2024.10.002","DOIUrl":"https://doi.org/10.1016/j.riam.2024.10.002","url":null,"abstract":"<p><strong>Background: </strong>Pneumocystis jirovecii colonization rates in healthy patients are unclear. Previously published studies suggest that the fungus could play a role in the physiopathology and progression of chronic respiratory diseases.</p><p><strong>Aims: </strong>The goal of this study was to determine the prevalence of colonization by this fungus in the lower respiratory tract of immunocompetent patients who are not at risk of dysbiosis.</p><p><strong>Methods: </strong>The presence of P. jirovecii was confirmed in the bronchoalveolar lavage (BAL) samples from adults who underwent bronchoscopy for non-infectious reasons, had no immunosuppressive factors, and had not been on antibiotic treatment for at least one month. The results were compared with those obtained in the study on the presence of Pneumocystis in environmental dust samples obtained by swabbing surfaces in the participating subjects' domestic settings. Real-time PCR was the technique used for detecting the fungus in both types of samples.</p><p><strong>Results: </strong>A total of 97 BAL samples and 49 domestic environment samples were studied. The medical reasons for needing a bronchoscopy were, mainly, the examination of both pulmonary neoplasm in 55 patients (57%) and diffuse interstitial lung disease in 21 patients (22%). The overall prevalence of P. jirovecii in our population was 7.22% in BAL samples and 0% in domestic samples.</p><p><strong>Conclusions: </strong>The presence of P. jirovecii in the lower respiratory tract is relevantly linked with the patient's immune status, not with an underlying pathology. Prevalence is low in immunocompetent individuals who do not have any infectious pathology and are not having antimicrobial treatments. Our results do not enable us to figure out which the environmental niche of P. jirovecii is.</p>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal ball due to a species of the Scedosporium apiospermum complex in a post-tuberculosis patient","authors":"Gabriela López Daneri ,&nbsp;Natali Niebles ,&nbsp;Agustina Bustos ,&nbsp;Pablo Martin Bravo ,&nbsp;Edith Susana Paterno ,&nbsp;Fernando Picco ,&nbsp;Alejandra Margari ,&nbsp;María Teresa Mujica","doi":"10.1016/j.riam.2024.07.001","DOIUrl":"10.1016/j.riam.2024.07.001","url":null,"abstract":"<div><h3>Background</h3><div><em>Scedosporium</em> species are considered emerging pathogens causing illness in immunocompetent and immunocompromised hosts.</div></div><div><h3>Case report</h3><div>A case of non-invasive pulmonary (fungal ball) infection by <em>Scedosporium apiospermum</em> complex in a 49-year-old female with bronchiectasis and cavities secondary to tuberculosis is described. The patient had a history of three years of cough and hemoptysis. A computed tomography scan of the thorax revealed the presence of a cavity in the lower lobe of the right lung, associated with bronchiectasis. A combination of surgical debridement and antifungal therapy (voriconazole) was the treatment of choice. Pulmonary resection (right lower lobectomy) was performed, and samples were sent for microbiological culture and histopathological examination; by means of the latter technique, hyphae were shown. The identification of <em>Scedosporium angustum</em>, a phylogenetic species of the <em>S. apiospermum</em> complex, was obtained by amplifying and sequencing the β-tubulin locus. Voriconazole therapy was started at a loading dose of 800<!--> <!-->mg/12<!--> <!-->h for the first 24<!--> <!-->h, followed by 200<!--> <!-->mg/12<!--> <!-->h for 6 months. The patient responded favorably to the treatment and remained asymptomatic.</div></div><div><h3>Conclusions</h3><div>This case emphasizes the importance of considering <em>Scedosporium</em> species in the differential diagnosis of fungal balls by <em>Aspergillus</em>.</div><div>.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"41 2","pages":"Pages 43-47"},"PeriodicalIF":1.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nails and skin co-infection by Fusarium verticillioides and Proteus vulgaris secondary to arterial occlusion of lower extremity","authors":"Yahui Feng ,&nbsp;Zhiya Yang ,&nbsp;Dengli Li ,&nbsp;Juanjuan Li ,&nbsp;Dongmei Li ,&nbsp;Sybren de Hoog ,&nbsp;Dongmei Shi","doi":"10.1016/j.riam.2021.07.003","DOIUrl":"10.1016/j.riam.2021.07.003","url":null,"abstract":"<div><h3>Background</h3><div>Post-traumatic <em>Fusarium</em> infection is rare. Arterial occlusive disease, a common vascular disorder in the elderly, often leads to ischemic necrosis of the lower extremities, which in turn increases the likelihood of secondary infections. Those secondary infections can be caused by bacteria, virus, or fungi.</div></div><div><h3>Case report</h3><div>We present the case of a 64-year-old male patient with a co-infection by <em>Fusarium verticillioides</em> and <em>Proteus vulgaris</em> on nails and foot skin, secondary to senile arterial occlusion on the lower extremities. The skin and nails recovered well after following stent implantation and a combination treatment of itraconazole and a macrolide antibiotic. A retrospective analysis of the literature identified 17 patients with <em>Fusarium</em> infection, all of whom were immunocompetent.</div></div><div><h3>Conclusions</h3><div>Trauma may be a predisposing cause of <em>Fusarium</em> infection. Combination of oral itraconazole and terbinafine, or amphotericin B and surgical means, are all effective treatments.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"41 2","pages":"Pages 37-42"},"PeriodicalIF":1.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aspergillus hiratsukae as a causative agent of endophthalmitis: A case report","authors":"Cristian Mateo León ,&nbsp;Ángeles Sampere Martínez ,&nbsp;Isabel Fabelo Hidalgo ,&nbsp;Diego García Martínez de Artola","doi":"10.1016/j.riam.2024.10.001","DOIUrl":"10.1016/j.riam.2024.10.001","url":null,"abstract":"<div><h3>Background</h3><div><em>Aspergillus flavus</em> and <em>Aspergillus fumigatus</em> are commonly involved in fungal endophthalmitis. However, other less pathogenic species of the genus are also causal agents of opportunistic infections. <em>Aspergillus hiratsukae</em>, a species within <em>Aspergillus</em> section <em>Fumigati</em>, is an uncommon fungus responsible for a few infectious cases worldwide.</div></div><div><h3>Case report</h3><div>We present a case of post-surgical endophthalmitis in a man caused by <em>A. hiratsukae</em> after phacoemulsification surgery and intraocular lens implantation. The patient underwent an urgent vitrectomy in which infectious foci were observed. Due to adverse clinical evolution, evisceration was performed five days after admission. In the study of the intraoperative samples fungal growth was observed. Microscopic analysis showed conidial heads resembling <em>Aspergillus</em> species. The final identification of <em>A. hiratsukae</em> was achieved by amplifying and sequencing the ITS1-5.8S-ITS2 spacer region. Antifungal susceptibility testing was performed using the method described by EUCAST. The isolate exhibited sensitivity to azoles and polyenes.</div></div><div><h3>Conclusions</h3><div>Due to the morphological similarity of the <em>Aspergillus</em> species, molecular techniques are essential for discriminating among them, as there are different antifungal susceptibility profiles within the same complex. Further studies to improve clinical surveillance, make an early diagnosis and start a targeted therapy are necessary.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"41 2","pages":"Pages 48-50"},"PeriodicalIF":1.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, virulence factors and antifungal susceptibility of oral isolates of Candida albicans from patients with cystic fibrosis in Mexico","authors":"Ana L. Ríos-López,&nbsp;María Fernanda Garza-Velásquez,&nbsp;Gloria M. González,&nbsp;Miguel A. Becerril-García,&nbsp;Orlando Flores-Maldonado","doi":"10.1016/j.riam.2024.09.001","DOIUrl":"10.1016/j.riam.2024.09.001","url":null,"abstract":"<div><h3>Background</h3><div><em>Candida</em> species are frequently isolated from the oral cavity of patients with cystic fibrosis. However, the information on the role of <em>Candida</em> in cystic fibrosis is scarce.</div></div><div><h3>Aims</h3><div>This study aimed to evaluate the prevalence, virulence profile and antifungal susceptibility of oral isolates of <em>Candida albicans</em> recovered from patients with cystic fibrosis.</div></div><div><h3>Methods</h3><div>Oropharyngeal swab samples were collected from sixty-five cystic fibrosis patients and sixty-five healthy individuals. <em>Candida</em> isolates were identified by MALDI-TOF VITEK-MS. Proteinase, phospholipase and esterase activity, biofilm production and level expression of <em>ALS</em>, <em>SAP</em> and <em>PLB</em> genes in <em>C. albicans</em> were evaluated. Minimal inhibitory concentration values were determined by means of an antifungal susceptibility test.</div></div><div><h3>Results</h3><div>Oral <em>Candida</em> colonization in cystic fibrosis patients was 66.15%, while in healthy individuals was 36.92%. <em>C. albicans</em> was the most frequently isolated species. <em>C. albicans</em> strains from cystic fibrosis patients were high producers of protease and biofilm, and had higher expression levels of adhesin and protease-associated genes in comparison with healthy subjects. Among the <em>C. albicans</em> strains isolated from cystic fibrosis patients, 18.91% were resistant to itraconazole, while 16.21% exhibited resistance to ketoconazole and fluconazole, and only one strain was resistant to voriconazole.</div></div><div><h3>Conclusions</h3><div>This work represents a surveillance study on virulence patterns and antifungal susceptibility of <em>Candida</em> from the oropharyngeal tract in cystic fibrosis.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"41 2","pages":"Pages 31-36"},"PeriodicalIF":1.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pneumocystis carinii infection drives upregulation of Fn1 expression that causes pulmonary fibrosis with an inflammatory response","authors":"Wenwen Yu ,&nbsp;Hua Ye ,&nbsp;Yunlei Li ,&nbsp;Xiaoqiong Bao,&nbsp;Yangyang Ni,&nbsp;Xiangxiang Chen,&nbsp;Yangjie Sun,&nbsp;Ali Chen,&nbsp;Weilong Zhou,&nbsp;Jifa Li","doi":"10.1016/j.riam.2024.04.002","DOIUrl":"10.1016/j.riam.2024.04.002","url":null,"abstract":"<div><h3>Background</h3><div><em>Pneumocystis carinii</em> is an opportunistic fungal pathogen that may cause pneumonia and lead to pulmonary fibrosis.</div></div><div><h3>Aims</h3><div>This study attempted to investigate the role of <em>P. carinii</em> infection-related genes in regulating lung fibrosis in mice.</div></div><div><h3>Methods</h3><div>A screening of <em>P. carinii</em> infection-related differential mRNAs was performed using the GEO database, followed by protein–protein interaction (PPI) network construction using the STRING website in order to obtain <em>P. carinii</em> infection-related key genes. The development of a mouse model with gene aberrant expression was achieved by utilizing mice carrying the Cre-LoxP recombinase system. Dexamethasone was employed to induce tracheal infection in order to develop a model of pulmonary fibrosis, and the magnitude of lung injury was assessed by performing hematoxylin–eosin (H&amp;E) staining and Masson staining. Lung coefficient and hydroxyproline level were assessed on sections of lung tissue as well. Finally, the magnitude of lung fibrosis and inflammation in mice was determined based on immunofluorescence and on the expression of genes associated with lung fibrosis and inflammation.</div></div><div><h3>Results</h3><div>Fn1 was found by PPI with the highest connectivity in the PPI network associated with immunity and inflammation. Besides, Fn1 was significantly highly expressed in <em>P. carinii</em>-infected mice samples. The <em>P carinii</em> pneumonia (PCP)+Fn1^fl/fl group had significantly higher lung coefficients, hydroxyproline levels and TNF-α, IL-6, IL-1β, IL-8 and NLRP3 expression levels, and significantly lower IL-10 expression levels. The results found in PCP+SPC-Cre:Fn1^fl/fl group were the opposite. The results of the pulmonary fibrosis level study showed that the PCP+Fn1^fl/fl group had the most intense H&amp;E and Masson staining, and significantly higher expression levels of Col1A2, Col3A1 and α-SMA, which were lower in the PCP+SPC-Cre:Fn1^fl/fl group.</div></div><div><h3>Conclusions</h3><div><em>P. carinii</em> infection may promote the upregulation of Fn1, which causes pulmonary fibrosis with an inflammatory response.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"41 1","pages":"Pages 17-26"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical response to prednisone in a severe case of infant-juvenile paracoccidioidomycosis","authors":"","doi":"10.1016/j.riam.2024.04.001","DOIUrl":"10.1016/j.riam.2024.04.001","url":null,"abstract":"<div><h3>Background</h3><div>Paracoccidioidomycosis is a neglected tropical disease caused by fungi of the genus <em>Paracoccidioides</em>. A wide range of symptoms is related to the disease; however, lungs and skin are the sites predominantly affected. The disease is mostly seen in people living in rural areas in Latin America.</div></div><div><h3>Case report</h3><div>We present a pediatric case of severe disseminated paracoccidioidomycosis that slowly responded to the antifungal treatment. Within three months, symptoms evolved into hepatosplenomegaly, necrotic cervical and abdominal lymph nodes, and splenic abscess. Clinical response to amphotericin B deoxycholate and itraconazole was slow, resulting in pleural and peritoneal cavity effusions, heart failure and shock. Amphotericin B deoxycholate was replaced by the liposomal formulation, with no response. Subsequently, prednisone was added to the treatment, which led to improvement in the clinical response. Serological <em>Paracoccidioides</em> antibody titers were atypical, with very low titers in the critical phase and significant increase during the convalescence phase. The infection was finally cleared up with amphotericin B deoxycholate, liposomal amphotericin B and the use of corticosteroids. Paracoccidioidomycosis serology was non-reactive two years post-discharge.</div></div><div><h3>Conclusions</h3><div>Due to the intense inflammatory response triggered by <em>Paracoccidioides</em> cells, giving low-dose prednisone for a short period of time modulated the inflammatory response and supported antifungal treatment.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"41 1","pages":"Pages 27-30"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungemia due to opportunistic fungi in patients with cancer","authors":"","doi":"10.1016/j.riam.2024.01.001","DOIUrl":"10.1016/j.riam.2024.01.001","url":null,"abstract":"<div><h3>Background</h3><div>Fungemia due to uncommon fungi and secondary to multiple risk factors has become an emergent health problem, particularly in oncology patients.</div></div><div><h3>Aims</h3><div>This study shows the following data collected during an 11-year period in a tertiary care oncologic center from patients with fungemia: demographic data, clinical characteristics, and outcome.</div></div><div><h3>Methods</h3><div>A retrospective study was performed at Instituto Nacional de Cancerología, a 135-bed referral cancer center in Mexico City, from July 2012 to June 2023. All episodes of non-<em>Candida</em> fungemia were included.</div></div><div><h3>Results</h3><div>Sixteen cases with uncommon fungemia were found in the database, representing 0.3% from all the blood cultures positive during the study period, and 8.5% from all the fungi isolated. The most common pathogens identified in our series were <span><span>Histoplasma capsulatum</span></span>, <span><span>Acremonium</span></span> spp., <span><span>Trichosporon asahii</span></span>, and <span><span>Saccharomyces cerevisiae</span></span><span>. Eight patients had hematologic malignancies, and five had severe neutropenia. In eight cases fungemia was considered catheter-related, in four cases was classified as primary, and in the last four it was diagnosed as disseminated fungal diseases. Mortality at 30 days was 43.8%.</span></div></div><div><h3>Conclusions</h3><div>The improved diagnostic tools have led to a better diagnosis of uncommon fungal infections. More aggressive therapeutic approaches, particularly in patients with malignancies, would increase survival rates in these potentially fatal diseases.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"41 1","pages":"Pages 1-6"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Azole resistance in a clinical isolate of Aspergillus fumigatus from Chile","authors":"Eduardo Álvarez Duarte ,&nbsp;Nicolás Cepeda ,&nbsp;Jean Miranda","doi":"10.1016/j.riam.2024.04.003","DOIUrl":"10.1016/j.riam.2024.04.003","url":null,"abstract":"<div><h3>Background</h3><div><em>Aspergillus fumigatus</em> is a ubiquitous opportunistic pathogen. This fungus can acquire resistance to azole antifungals due to different mutations in the cyp51A gene. Azole resistance has been observed in several continents and appears to be a globally distributed phenomenon. Specific mutations in cyp51A that lead to azole resistance, such as the TR34/L98H modification, have been reported.</div></div><div><h3>Aims</h3><div>To evaluate the azole resistance in clinically isolated <em>A. fumigatus</em> strains.</div></div><div><h3>Methods</h3><div>As a result of our passive surveillance strategy, a total of 23 <em>A. fumigatus</em> isolates from clinical origins were identified through a phylogenetic analysis using the ITS region and β-tubulin gene fragments, and typed with the CSP microsatellite. Azole susceptibility profiles were performed by disk diffusion and microdilution broth methodologies according to CLSI guidelines.</div></div><div><h3>Results</h3><div>Here we describe, for the first time, the detection of azole-resistant <em>A. fumigatus</em> isolates from clinical origins in Chile with mutations in the cyp51A gene. In addition to the TR34/L98H mutation, one isolate exhibited an F46Y/M172V/E427K-type mutation. Furthermore, microsatellite typing based on cell surface protein (CSP) was performed, showing the t02 (TR34/L98H), t15 (F46Y/M172V/E427K) and t01 (susceptible clinical isolates) genotypes.</div></div><div><h3>Conclusions</h3><div>Our study demonstrates the presence of mutations related to azole resistance in <em>A. fumigatus</em> strains isolated from clinical samples in Chile. In order to obtain information that may help to tackle the spread of antifungal resistance among <em>A. fumigatus</em> populations, and to ensure the efficacy of future treatments against aspergillosis, a further research is necessary.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"41 1","pages":"Pages 7-12"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental study of specific and nonspecific blood culture bottles for the diagnosis of candidemia","authors":"Leandre Carmem Wilot ,&nbsp;Vanice Rodrigues Poester ,&nbsp;Cecília Bittencourt Severo ,&nbsp;Karine Ortiz Sanchotene ,&nbsp;Bruna Muradás Esperon ,&nbsp;Mariana Rodrigues Trápaga ,&nbsp;David A. Stevens ,&nbsp;Melissa Orzechowski Xavier","doi":"10.1016/j.riam.2024.06.001","DOIUrl":"10.1016/j.riam.2024.06.001","url":null,"abstract":"<div><h3>Background</h3><div>Early diagnosis of candidemia is critical for the correct management and treatment of patients.</div></div><div><h3>Aims</h3><div>To test the efficacy of different blood culture bottles in the growth of <em>Candida</em> strains.</div></div><div><h3>Methods</h3><div>We compared the performance of BD BACTEC™ Plus Aerobic/F (Aero) culture bottles with the specific BD BACTEC™ Mycosis IC/F Lytic (Myco) culture bottles using the BD BACTEC™ FX 40 automated blood culture system to determine the mean time-to-detection (TTD) in <em>Candida</em> species. One isolate each of six <em>Candida</em> species was inoculated into blood culture bottles (final concentration, 1–5<!--> <!-->CFU<!--> <!-->ml⁻¹) and incubated at 37<!--> <!-->°C until automated growth detection.</div></div><div><h3>Results</h3><div><em>Candida albicans</em> and <em>Nakaseomyces glabratus</em> (<em>Candida glabrata</em>) were detected earlier in the specific culture bottle, whereas <em>Candida tropicalis</em> was detected earlier in the nonspecific bottle; <em>Candida parapsilosis</em>, <em>Pichia kudriavzevii</em> (<em>Candida krusei</em>), and <em>Meyerozyma guilliermondii</em> (<em>Candida guilliermondii</em>) presented similar TTD in both bottles.</div></div><div><h3>Conclusions</h3><div>Our study suggests the suitability of using both bottles in clinical laboratories for a faster diagnosis and prompt starting of any treatment.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"41 1","pages":"Pages 13-16"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}