Revista Iberoamericana De Micologia最新文献_第10页

Infección del torrente sanguíneo por Candida glabrata en una unidad de cuidados intensivos neonatales con prescripción de profilaxis con fluconazol 新生儿重症监护病房使用氟康唑预防的光滑念珠菌血流感染

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2020-07-01 DOI: 10.1016/j.riam.2020.06.002

Juan Carlos Lona-Reyes , Larissa María Gómez-Ruiz , Araceli Cordero-Zamora , Mariana Chávez-Rodríguez , Juan Antonio Gallegos-Marín , Denisse Sinaí Jiménez-Hernández

{"title":"Infección del torrente sanguíneo por Candida glabrata en una unidad de cuidados intensivos neonatales con prescripción de profilaxis con fluconazol","authors":"Juan Carlos Lona-Reyes , Larissa María Gómez-Ruiz , Araceli Cordero-Zamora , Mariana Chávez-Rodríguez , Juan Antonio Gallegos-Marín , Denisse Sinaí Jiménez-Hernández","doi":"10.1016/j.riam.2020.06.002","DOIUrl":"https://doi.org/10.1016/j.riam.2020.06.002","url":null,"abstract":"<div><h3>Background</h3><p><em>Candida glabrata</em> is an emerging pathogen with the ability to develop tolerance and resistance to azole antifungals, which creates uncertainty about the usefulness of antifungal prophylaxis in newborns.</p></div><div><h3>Aims</h3><p>The aim of this study was to describe the factors associated with <em>C. glabrata</em> infection in a NICU that uses prophylaxis with fluconazole.</p></div><div><h3>Methods</h3><p>A case-control study paired by gestational age was designed and conducted at the Civil Hospital of Guadalajara Dr. Juan I. Menchaca. Newborns with <em>C. glabrata</em> infection were studied and for each one a matched control was selected by gestational age. Odds ratios (OR) were estimated with 95% confidence intervals (95% CI) and McNemar test for contrast of hypothesis was applied.</p></div><div><h3>Results</h3><p>Twenty-one infected patients were identified, from whom 66.7% were male; the median gestational age was 31.5 weeks. Increased risk of infection with <em>C. glabrata</em> was observed when there was a prescription of more than one antimicrobial scheme (OR 21, 95% CI, 1.23 - 358.3; <em>p</em>  =0.04). During the study period, exposure to fluconazole showed no difference in the risk of infection.</p></div><div><h3>Conclusions</h3><p>Neonates with more than one antimicrobial regimen and those with surgical comorbidities had a higher risk of <em>C. glabrata</em> infection.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"37 3","pages":"Pages 100-103"},"PeriodicalIF":1.9,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.06.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92027428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Identification of Mucor circinelloides antigens recognized by sera from immunocompromised infected mice 免疫功能低下感染小鼠血清中环状毛霉抗原的鉴定

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2020-07-01 DOI: 10.1016/j.riam.2020.07.002

Maialen Areitio , Adela Martin-Vicente , Aitana Arbizu , Aitziber Antoran , Leire Aparicio-Fernandez , Idoia Buldain , Leire Martin-Souto , Aitor Rementeria , Javier Capilla , Fernando L. Hernando , Andoni Ramirez-Garcia

{"title":"Identification of Mucor circinelloides antigens recognized by sera from immunocompromised infected mice","authors":"Maialen Areitio , Adela Martin-Vicente , Aitana Arbizu , Aitziber Antoran , Leire Aparicio-Fernandez , Idoia Buldain , Leire Martin-Souto , Aitor Rementeria , Javier Capilla , Fernando L. Hernando , Andoni Ramirez-Garcia","doi":"10.1016/j.riam.2020.07.002","DOIUrl":"10.1016/j.riam.2020.07.002","url":null,"abstract":"<div><h3>Background</h3><p><span><em>Mucor circinelloides</em></span> is an opportunistic fungus capable of causing mucormycosis, a highly aggressive infection of quick spreading. Besides, it also has a high mortality rate due to late diagnosis and difficult treatment.</p></div><div><h3>Aims</h3><p>In this study we have identified the most immunoreactive proteins of the secretome and the total protein extract of <em>M. circinelloides</em> using sera from immunocompromised infected mice.</p></div><div><h3>Methods</h3><p><span>The proteins of the secretome and the total extract were analyzed by two-dimensional electrophoresis and the most immunoreactive antigens were detected by Western Blot, facing the sera of immunocompromised infected mice to the proteins obtained in both extracts of </span><em>M. circinelloides</em>.</p></div><div><h3>Results</h3><p>Seven antigens were detected in the secretome extract, and two in the total extract, all of them corresponding only to three proteins. The enzyme enolase was detected in both extracts, while triosephosphate isomerase was detected in the secretome, and heat shock protein HSS1 in the total extract.</p></div><div><h3>Conclusions</h3><p>In this work the most immunoreactive antigens of the secretome and the total extract of <em>M. circinelloides</em> were identified. The identified proteins are well known fungal antigens and, therefore, these findings can be useful for future research into alternatives for the diagnosis and treatment of mucormycosis.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"37 3","pages":"Pages 81-86"},"PeriodicalIF":1.9,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.07.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38679094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Guttural pouch mycosis, sympathy for Aspergillus nidulans 咽喉袋真菌病，与细曲霉同感

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2020-07-01 DOI: 10.1016/j.riam.2020.01.002

F. Javier Cabañes

引用次数: 1

Synergistic interactions between β-lapachone and fluconazole in the inhibition of CaCdr2p and CaMdr1p in Candida albicans β-拉帕酮与氟康唑对白色念珠菌CaCdr2p和CaMdr1p抑制的协同作用

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2020-07-01 DOI: 10.1016/j.riam.2020.09.002

Daniel Clemente Moraes , Leandro Figueira Reis de Sá , Levy Tenorio Sousa Domingos , Maria do Carmo Freire Ribeiro Pinto , Rosangela Maria de Araújo Soares , Antônio Ferreira-Pereira

{"title":"Synergistic interactions between β-lapachone and fluconazole in the inhibition of CaCdr2p and CaMdr1p in Candida albicans","authors":"Daniel Clemente Moraes , Leandro Figueira Reis de Sá , Levy Tenorio Sousa Domingos , Maria do Carmo Freire Ribeiro Pinto , Rosangela Maria de Araújo Soares , Antônio Ferreira-Pereira","doi":"10.1016/j.riam.2020.09.002","DOIUrl":"10.1016/j.riam.2020.09.002","url":null,"abstract":"<div><h3>Background</h3><p>Mortality rate of invasive <em>Candida</em> infections is raising mainly amongst immunocompromised patients. These infections are hard-to-treat mainly due to the increasing incidence of resistance. The overexpression of ATP-binding cassette and major facilitator superfamily transporters is the main responsible for the failure of antifungal therapies. In a <span><em>Saccharomyces cerevisiae</em></span> model, β-lapachone inhibited Pdr5p, a transporter homologous to those found in <span><em>Candida albicans</em></span>.</p></div><div><h3>Aims</h3><p>To determine whether β-lapachone reverses the resistance phenotype mediated by efflux transporters in <em>C</em>. <em>albicans</em> clinical isolates.</p></div><div><h3>Methods</h3><p>The antifungal activity of β-lapachone combined with fluconazole was measured by agarose chemosensitization and microdilution assays. CaCdr2p and CaMdr1p activities were evaluated through fluorescent dyes accumulation. ATPase activity was assessed using transporter-enriched plasma membranes.</p></div><div><h3>Results</h3><p>β-lapachone reverted antifungal resistance of <em>S. cerevisiae</em> and <em>C. albicans</em> strains overexpressing CaCdr2p and CaMdr1p transporters by inhibiting these proteins activities. CaCdr2p ATPase activity was not impaired by the compound.</p></div><div><h3>Conclusions</h3><p><span>β-lapachone is a promising drug candidate to be used as an adjuvant in the treatment of candidiasis caused by fluconazole-resistant </span><em>C. albicans</em> strains.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"37 3","pages":"Pages 104-106"},"PeriodicalIF":1.9,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.09.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40455514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Bat white-nose syndrome: A devastating epizootic in North America 蝙蝠白鼻综合征:北美一种毁灭性的动物流行病

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2020-07-01 DOI: 10.1016/j.riam.2020.01.003

F. Javier Cabañes

引用次数: 0

Recent changes in candidemia trends in a tertiary hospital (2011–2018) 某三级医院念珠菌流行趋势近期变化(2011-2018年)

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2020-07-01 DOI: 10.1016/j.riam.2020.09.005

Ana I. Piqueras , Thomas Rubio , Eva M. Lopez-Medina , Ana Gimeno , Vicent Modesto , Emilia Cantón , Javier Pemán

{"title":"Recent changes in candidemia trends in a tertiary hospital (2011–2018)","authors":"Ana I. Piqueras , Thomas Rubio , Eva M. Lopez-Medina , Ana Gimeno , Vicent Modesto , Emilia Cantón , Javier Pemán","doi":"10.1016/j.riam.2020.09.005","DOIUrl":"10.1016/j.riam.2020.09.005","url":null,"abstract":"<div><h3>Background</h3><p>The epidemiology of candidemia has changed over the last decades and varies widely among geographic areas.</p></div><div><h3>Aims</h3><p>We examined in children (aged 0–14) with candidemia the trends in the incidence rate of this infection, as well as the clinical characteristics of the patients, in order to optimize the prognosis and the control measures of this serious disease.</p></div><div><h3>Methods</h3><p>A retrospective cohort study of candidemia in the period 2011–2018 in the neonatal intensive care unit (NICU), pediatric ICU (PICU) and pediatric wards of a tertiary hospital, was conducted. The clinical course, <em>Candida</em> species isolated, antifungal susceptibility, outcome and incidence rates were analyzed and compared.</p></div><div><h3>Results</h3><p>We diagnosed 68 episodes of candidemia in 62 children, 48% occurred in the NICU, 31% in the PICU and 21% in pediatric wards. <span><em>Candida albicans</em></span> was the most frequent species isolated in NICU infants (53%), and <span><em>Candida parapsilosis</em></span><span> predominated among PICU patients (59%) and pediatric wards (50%). One third of NICU infants had invasive candidiasis (IC), most of them having extremely low birth weight (ELBW) (35%). All isolates were susceptible to the antifungal administered. Over time, the incidence of candidemia decreased in the PICU (from 2.2 to 0.3 episodes/1000 patient-days, OR</span>  <!-->0.6; 95%CI 0.5–0.8), whereas in the NICU and in the wards remained stable. Mortality occurred mostly in NICU patients (26%), predominated in ELBW infants and did not change over time.</p></div><div><h3>Conclusions</h3><p>The higher incidence and mortality of candidemia and IC observed in preterm infants requires a continuous evaluation of practices and diagnostic methods which will allow improving the prognosis of this most vulnerable population.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"37 3","pages":"Pages 87-93"},"PeriodicalIF":1.9,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.09.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38339678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Infección fúngica sinusal por Schizophyllum commune: a propósito de dos casos clínicos 常见裂叶菌鼻窦真菌感染:两例临床病例的综述

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2020-04-01 DOI: 10.1016/j.riam.2020.02.004

Belén Fernández-Caso , Arturo Manuel Fraile , Marta Soledad Rodríguez , Inmaculada Fernández , Buenaventura Buendía

{"title":"Infección fúngica sinusal por Schizophyllum commune: a propósito de dos casos clínicos","authors":"Belén Fernández-Caso , Arturo Manuel Fraile , Marta Soledad Rodríguez , Inmaculada Fernández , Buenaventura Buendía","doi":"10.1016/j.riam.2020.02.004","DOIUrl":"10.1016/j.riam.2020.02.004","url":null,"abstract":"<div><h3>Background</h3><p><em>Schizophyllum commune</em> is a basidiomycete fungus which is widely distributed in nature. Its role as responsible for disease in humans is not well known, partly due to its difficult identification. The incorporation of mass spectrometry techniques (MALDI-TOF) and molecular biology to the laboratories has allowed the description of a greater number of cases.</p></div><div><h3>Case report</h3><p>In this paper, we present two cases in which <em>S. commune</em> was identified as the causative agent of disease: in the first case an immunocompetent patient suffered from chronic rhinosinusitis, and in the second one a sphenoid sinus infection was diagnosed in an immunocompromised patient. In both cases, <em>S. commune</em> was isolated. Its identification was possible by means of MALDI-TOF and this was confirmed in both patients by amplification and sequencing of the ITS region.</p></div><div><h3>Conclusions</h3><p>In conclusion, <em>S. commune</em> should be considered a potential causative agent of fungal disease. Currently, MALDI-TOF and sequencing techniques are necessary for its identification.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"37 2","pages":"Pages 65-67"},"PeriodicalIF":1.9,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.02.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37909114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micosis pulmonares en pacientes con diabetes mellitus. Características clínicas y factores de riesgo 糖尿病患者肺真菌病。临床特征及危险因素

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2020-04-01 DOI: 10.1016/j.riam.2020.04.002

Alejandro Hernández-Solís , Alejandra Camerino Guerrero , Yesenia Colín Muñoz , Saret Bazán Cuervo , Raúl Cícero Sabido , Arturo Reding-Bernal

{"title":"Micosis pulmonares en pacientes con diabetes mellitus. Características clínicas y factores de riesgo","authors":"Alejandro Hernández-Solís , Alejandra Camerino Guerrero , Yesenia Colín Muñoz , Saret Bazán Cuervo , Raúl Cícero Sabido , Arturo Reding-Bernal","doi":"10.1016/j.riam.2020.04.002","DOIUrl":"10.1016/j.riam.2020.04.002","url":null,"abstract":"<div><h3>Background</h3><p>Diabetes mellitus is a public health problem in Mexico, and the trend of the disease is increasing. From 2000 to 2017, 7.32 million new cases were diagnosed, with pulmonary mycoses being one of the most serious complications.</p></div><div><h3>Aims</h3><p>To describe the frequency and the clinical characteristics of patients diagnosed with pulmonary mycoses, and to identify the risk factors associated with this entity.</p></div><div><h3>Methods</h3><p>Case–control study, paired by gender (1:1-3) and age (± 5 years), that analyzed patients with pulmonary mycosis (mucormycosis, histoplasmosis, coccidioidomycosis, blastomycosis, aspergillosis, cryptococcosis, paracoccidioidomycosis) and studied the risk factors present in each patient.</p></div><div><h3>Results</h3><p>From the 162 patients studied, 56 suffered pulmonary mycosis and 106 were controls. The median of the age was 51 and 50 years for the cases and for the controls, respectively. Multiple logistic regression analysis showed that patients with diabetes mellitus had an odds ratio of 8,3 <em>(p</em> < 0.001), and patients with a history of tuberculosis had an odds ratio of 8,8 <em>(p</em> < 0.001).</p></div><div><h3>Conclusions</h3><p>Our results show that 52% of the patients with pulmonary mycoses had a history of diabetes mellitus. Diabetes mellitus is a relevant risk factor for pulmonary mycoses, which are usually diagnosed in advanced stages and have a high mortality.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"37 2","pages":"Pages 53-57"},"PeriodicalIF":1.9,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.04.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38113315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Actividad antifúngica de los enjuagues bucales frente a Candida albicans y Rhodotorula mucilaginosa: un estudio in vitro 漱口水对白色念珠菌和黏液红藻的抗真菌活性:一项体外研究

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2020-04-01 DOI: 10.1016/j.riam.2019.10.006

Rodrigo Alejandro Handschuh Briones , Evelyn Nicole Silva Arcos , Milton Urrutia , Patricio Godoy-Martínez

{"title":"Actividad antifúngica de los enjuagues bucales frente a Candida albicans y Rhodotorula mucilaginosa: un estudio in vitro","authors":"Rodrigo Alejandro Handschuh Briones , Evelyn Nicole Silva Arcos , Milton Urrutia , Patricio Godoy-Martínez","doi":"10.1016/j.riam.2019.10.006","DOIUrl":"10.1016/j.riam.2019.10.006","url":null,"abstract":"<div><h3>Background</h3><p><em>Candida albicans</em> and <em>Rhodotorula mucilaginosa</em> are yeasts of clinical importance in the oral cavity. In immunocompromised patients they can cause some pathologies that must be controlled with antimicrobials.</p></div><div><h3>Aims</h3><p>To evaluate and compare the antimicrobial efficacy of commercially available mouthrinses against strains of <em>C. albicans</em> and <em>R. mucilaginosa</em>.</p></div><div><h3>Methods</h3><p>The six mouthwashes studied <em>in vitro</em> were formulated (alone or in combination) with chlorhexidine (CHX) 0.12%, CHX 0.1%, CHX 0.05%, cetylpyridinium chloride (CPC) 0.075%, CPC 0.05%, and essential oils. Ten <em>C. albicans</em> and <em>R. mucilaginosa</em> isolates each were studied. The agar diffusion method (Mueller Hinton II), with incubation at 32°C was used to evaluate the antifungal activity.</p></div><div><h3>Results</h3><p>The results of this study indicate that mouthwashes with CHX 0.1%, CHX 0.12%, CHX 0.05% + CPC 0.05%, CHX 0.12% + CPC 0.05% and CPC 0.075% have an antifungal effect against <em>C. albicans</em> and <em>R. mucilaginosa</em>. CHX 0.1% led to the broadest inhibition zone for <em>C. albicans</em> and <em>R. mucilaginosa</em> (25.65±2.39mm and 40.05±3.31mm). Essential oils did not show any antifungal activity. Statistical analysis showed no statistical difference between mouth rinses CHX 0.1%, CHX 0.12% and CHX 0.12% + CPC 0.05% (<em>p</em>  <!-->0.0001) against <em>C. albicans</em> and <em>R. mucilaginosa</em>.</p></div><div><h3>Conclusions</h3><p>Mouthwashes with CHX showed higher antifungal activity against <em>C. albicans</em> and <em>R. mucilaginosa</em> than other mouthwashes studied.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"37 2","pages":"Pages 47-52"},"PeriodicalIF":1.9,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2019.10.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38198998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tsunamis and changes in the etiology of the canine and feline cryptococcosis 海啸和犬猫隐球菌病病因学的变化

IF 1.9 4区生物学

Revista Iberoamericana De Micologia Pub Date : 2020-04-01 DOI: 10.1016/j.riam.2019.12.002

F. Javier Cabañes

引用次数: 0