念珠菌的基因型与毒力之间缺乏相关性

IF 1.5 4区 生物学 Q4 MYCOLOGY
Judith Díaz-García , Maiken C. Arendrup , Rafael Cantón , Julio García-Rodríguez , Elia Gómez García de la Pedrosa , Gabriella Parisi , Javier Pemán , Brunella Posteraro , Maurizio Sanguinetti , Daniel Archimedes Da Matta , Arnaldo L. Colombo , Patricia Muñoz , Carlos Sánchez-Carrillo , Jesús Guinea , Pilar Escribano
{"title":"念珠菌的基因型与毒力之间缺乏相关性","authors":"Judith Díaz-García ,&nbsp;Maiken C. Arendrup ,&nbsp;Rafael Cantón ,&nbsp;Julio García-Rodríguez ,&nbsp;Elia Gómez García de la Pedrosa ,&nbsp;Gabriella Parisi ,&nbsp;Javier Pemán ,&nbsp;Brunella Posteraro ,&nbsp;Maurizio Sanguinetti ,&nbsp;Daniel Archimedes Da Matta ,&nbsp;Arnaldo L. Colombo ,&nbsp;Patricia Muñoz ,&nbsp;Carlos Sánchez-Carrillo ,&nbsp;Jesús Guinea ,&nbsp;Pilar Escribano","doi":"10.1016/j.riam.2020.10.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The virulence of isolates among different <em>Candida</em><span> species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied.</span></p></div><div><h3>Aims</h3><p>We aim to assess the relationship between genotype and virulence in <em>Candida</em> species using a <span><em>Galleria mellonella</em></span> larvae infection model.</p></div><div><h3>Methods</h3><p>One hundred and ninety-four isolates from 68 clusters (<span><em>Candida albicans</em></span>, 114/41; <span><em>Candida parapsilosis</em></span>, 74/24; <span><em>Candida tropicalis</em></span>, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of <em>G. mellonella</em> larvae.</p></div><div><h3>Results</h3><p>The median of survival and the IQR ranges of clusters and singleton were as follows: <em>C. albicans</em> (2 days, IQR 1.5–2 <em>vs.</em> 2 days, IQR 1–2.25), <em>C. parapsilosis</em> (2 days, IQR 1.5–2.6 <em>vs.</em> 2 days, IQR 2–3.3), and <em>C. tropicalis</em> (1 day, IQR 1–3.5 <em>vs.</em> 2 days, IQR 2–3.5; <em>p</em> <!-->&lt;<!--> <!-->0.05). High intra-cluster variability in terms of median of survival was found regardless the species.</p></div><div><h3>Conclusions</h3><p>No relationship between genotype and virulence in <em>Candida</em> was observed with the <em>G. mellonella</em> model.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.10.002","citationCount":"0","resultStr":"{\"title\":\"Lack of relationship between genotype and virulence in Candida species\",\"authors\":\"Judith Díaz-García ,&nbsp;Maiken C. Arendrup ,&nbsp;Rafael Cantón ,&nbsp;Julio García-Rodríguez ,&nbsp;Elia Gómez García de la Pedrosa ,&nbsp;Gabriella Parisi ,&nbsp;Javier Pemán ,&nbsp;Brunella Posteraro ,&nbsp;Maurizio Sanguinetti ,&nbsp;Daniel Archimedes Da Matta ,&nbsp;Arnaldo L. Colombo ,&nbsp;Patricia Muñoz ,&nbsp;Carlos Sánchez-Carrillo ,&nbsp;Jesús Guinea ,&nbsp;Pilar Escribano\",\"doi\":\"10.1016/j.riam.2020.10.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>The virulence of isolates among different <em>Candida</em><span> species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied.</span></p></div><div><h3>Aims</h3><p>We aim to assess the relationship between genotype and virulence in <em>Candida</em> species using a <span><em>Galleria mellonella</em></span> larvae infection model.</p></div><div><h3>Methods</h3><p>One hundred and ninety-four isolates from 68 clusters (<span><em>Candida albicans</em></span>, 114/41; <span><em>Candida parapsilosis</em></span>, 74/24; <span><em>Candida tropicalis</em></span>, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of <em>G. mellonella</em> larvae.</p></div><div><h3>Results</h3><p>The median of survival and the IQR ranges of clusters and singleton were as follows: <em>C. albicans</em> (2 days, IQR 1.5–2 <em>vs.</em> 2 days, IQR 1–2.25), <em>C. parapsilosis</em> (2 days, IQR 1.5–2.6 <em>vs.</em> 2 days, IQR 2–3.3), and <em>C. tropicalis</em> (1 day, IQR 1–3.5 <em>vs.</em> 2 days, IQR 2–3.5; <em>p</em> <!-->&lt;<!--> <!-->0.05). High intra-cluster variability in terms of median of survival was found regardless the species.</p></div><div><h3>Conclusions</h3><p>No relationship between genotype and virulence in <em>Candida</em> was observed with the <em>G. mellonella</em> model.</p></div>\",\"PeriodicalId\":21291,\"journal\":{\"name\":\"Revista Iberoamericana De Micologia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.riam.2020.10.002\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Iberoamericana De Micologia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1130140620300504\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Iberoamericana De Micologia","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1130140620300504","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

背景:不同念珠菌种类引起念珠菌病的分离株的毒力可能影响患者的预后。此外,基因型与毒力之间的潜在关系尚不清楚,需要进一步研究。目的通过建立成熟Galleria mellonella幼虫感染模型,研究念珠菌的基因型与毒力之间的关系。方法从68个菌群中分离194株(白色念珠菌114/41;假丝酵母菌,74/24;将热带假丝酵母(6/3)与相同数量的单基因型菌株进行比较。结果聚集性和单例病例的生存中位数和IQR范围分别为:白色念珠菌(2天,IQR 1.5-2比2天,IQR 1 - 2.25),傍肺念珠菌(2天,IQR 1.5-2.6比2天,IQR 2 - 3.3),热带念珠菌(1天,IQR 1 - 3.5比2天,IQR 2 - 3.5;p & lt;0.05)。无论物种如何,在生存中位数方面都发现了高簇内变异性。结论假丝酵母基因型与毒力之间无相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lack of relationship between genotype and virulence in Candida species

Background

The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied.

Aims

We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model.

Methods

One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae.

Results

The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5–2 vs. 2 days, IQR 1–2.25), C. parapsilosis (2 days, IQR 1.5–2.6 vs. 2 days, IQR 2–3.3), and C. tropicalis (1 day, IQR 1–3.5 vs. 2 days, IQR 2–3.5; p < 0.05). High intra-cluster variability in terms of median of survival was found regardless the species.

Conclusions

No relationship between genotype and virulence in Candida was observed with the G. mellonella model.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
81 days
期刊介绍: Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信