Revista Iberoamericana De Micologia最新文献

{"title":"Pneumocystis in dogs: A protozoan knockin’ on Fungi's door","authors":"Francisco Javier Cabañes","doi":"10.1016/j.riam.2021.04.001","DOIUrl":"10.1016/j.riam.2021.04.001","url":null,"abstract":"","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 155-156"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2021.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38968859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Filamentous fungi in the airway of patients with cystic fibrosis: Just spectators?","authors":"Clara Viñado ,&nbsp;Rosa María Girón ,&nbsp;Elisa Ibáñez ,&nbsp;Alberto García-Ortega ,&nbsp;Inés Pérez ,&nbsp;Dinora Polanco ,&nbsp;Javier Pemán ,&nbsp;Amparo Solé","doi":"10.1016/j.riam.2021.03.005","DOIUrl":"10.1016/j.riam.2021.03.005","url":null,"abstract":"<div><h3>Background</h3><p>There are important advances in the management of bacterial infection in patients with cystic fibrosis (CF), but there are many gaps in the field of fungal infections.</p></div><div><h3>Aims</h3><p>The aim of this study was to analyse whether chronic respiratory filamentous fungal colonization had clinical impact and whether antifungal treatment can change the disease.</p></div><div><h3>Methods</h3><p>The prospective, bicentric and descriptive study was carried out within a 3-year follow-up period, with four-month periodicity medical controls. Adult patients from two CF units of tertiary hospitals were included. Clinical, microbiological, analytical and spirometric variables were collected. Quality of life was evaluated in a subgroup, using the Spanish version of the Revised Cystic Fibrosis Quality of Life Questionnaire (CFQ-R). To statistically analyze the evolution of forced expiratory along time (volume of air blown out in 1 second -FEV₁-) and the forced vital capacity (FVC), mixed linear models were carried out.</p></div><div><h3>Results</h3><p>From the ninety-eight patients under study, 40 suffered chronic filamentous fungal colonization. The presence of filamentous fungi in airway was associated to an annual fall of FEV₁<span> and FVC of 0.029 and 0.017 litres, respectively (p&lt;0.001). In addition, worse quality of life based on CFQ-R, significant when concerning physical condition and emotional state, was also linked with the fungal colonization. Protocolized antifungal therapy, nebulized or oral, improved FEV</span>₁ in 0.023 and 0.024 litres per year, respectively (p&lt;0.001).</p></div><div><h3>Conclusions</h3><p>Chronic filamentous fungal colonization in patients with CF is associated with a significant annual decline of lung function that persists over time. Chronic antifungal therapy slows down this progression, mainly in the patient with more advanced disease.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 168-174"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39427063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasive scedosporiosis in lung transplant recipients: A nine-year retrospective study in a tertiary care hospital","authors":"Elisa Ibáñez-Martínez ,&nbsp;Amparo Solé ,&nbsp;Antonio Cañada-Martínez ,&nbsp;Carlos F. Muñoz-Núñez ,&nbsp;Amparo Pastor ,&nbsp;Beatriz Montull ,&nbsp;Patricia Falomir-Salcedo ,&nbsp;Amparo Valentín ,&nbsp;José Luis López-Hontangas ,&nbsp;Javier Pemán","doi":"10.1016/j.riam.2021.06.002","DOIUrl":"10.1016/j.riam.2021.06.002","url":null,"abstract":"<div><h3>Background</h3><p><em>Scedosporium</em> species and <em>Lomentospora prolificans</em> (<em>Sc/Lp</em>) are emerging molds that cause invasive disease associated with a high mortality rate. After <em>Aspergillus</em>, these molds are the second filamentous fungi recovered in lung transplant (LT) recipients.</p></div><div><h3>Aims</h3><p>Our objective was to evaluate the incidence, risk factors and outcome of <em>Sc/Lp</em> infections in LT recipients at a tertiary care hospital with a national reference LT program.</p></div><div><h3>Methods</h3><p>A nine-year retrospective study was conducted.</p></div><div><h3>Results</h3><p>During this period, 395 LT were performed. Positive cultures for <em>Sc/Lp</em> were obtained from twenty-one LT recipients. Twelve patients (incidence 3.04%) developed invasive scedosporiosis (IS). In 66.7% of the patients with IS the invasive infection was defined as a breakthrough one. The main sites of infection were lungs and paranasal sinuses. Most of the patients received combination antifungal therapy. The IS crude mortality rate after 30 days was 16.7%, and 33.3% after a year.</p></div><div><h3>Conclusions</h3><p>Our study highlights improved survival rates associated with combination antifungal therapy in LT recipients and underlines the risk of breakthrough infections in patients with allograft dysfunction on nebulized lipidic amphotericin B prophylaxis. In addition to pretransplant colonization, acute or chronic organ dysfunctions seem to be the main risk factors for IS.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 184-187"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39512509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, enzymatic activity and biofilm formation of Candida species isolated from goat milk","authors":"Carolina Segundo Zaragoza ,&nbsp;Itzel López Ortiz ,&nbsp;David Alejandro Contreras Caro del Castillo ,&nbsp;Yesmín María Domínguez Hernández ,&nbsp;Juan Antonio Rodríguez García","doi":"10.1016/j.riam.2021.06.001","DOIUrl":"10.1016/j.riam.2021.06.001","url":null,"abstract":"<div><h3>Background</h3><p>Data regarding yeast microbiota in goat milk is scarce.</p></div><div><h3>Aims</h3><p>To isolate and identify species of the genus <em>Candida</em> in milk samples from clinically healthy goats, and evaluate their enzymatic activity and biofilm formation.</p></div><div><h3>Methods</h3><p>1092 milk samples from clinically healthy goats were collected and processed. The yeast isolates were identified by phenotypic, methods and their enzymatic activity (phospholipase, hemolysin and protease) and biofilm formation evaluated.</p></div><div><h3>Results</h3><p>We obtained 221 <em>Candida</em> isolates belonging to six species: <em>Candida kefyr</em> (35.7%), <em>Candida guilliermondii</em> (33%), <em>Candida famata</em> (23.5%), <em>Candida glabrata</em> (5.9%), <em>Candida albicans</em> (1.35%) and <em>Candida parapsilosis</em> <em>sensu lato</em> (0.45%). Protease activity was detected in all <em>Candida</em> species while hemolysin activity was only present in <em>C. kefyr, C. guilliermondii</em>, <em>C. famata</em> and <em>C. albicans</em>. Only <em>C. albicans</em> showed phospholipase activity. With the exception of <em>C. parapsilosis sensu lato</em>, all <em>Candida</em> species formed biofilm, with 60.19% of the isolates being poor producers, 9.93% moderate producers, and 1.35% strong producers.</p></div><div><h3>Conclusions</h3><p>The milk of clinically healthy goats contains several species of the genus <em>Candida</em> that could play a role as opportunistic pathogens in mastitis.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 175-179"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2021.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39407771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyberlindnera fabianii: primer aislamiento clínico en Chile","authors":"Eduardo Álvarez Duarte ,&nbsp;Nicolás Cepeda","doi":"10.1016/j.riam.2021.04.009","DOIUrl":"https://doi.org/10.1016/j.riam.2021.04.009","url":null,"abstract":"<div><h3>Background</h3><p>Although considered an unusual etiological agent, <em>Cyberlindnera</em> <em>(Candida)</em> <em>fabianii</em> has been related to septicemia in several reports in recent years. Its doubtful or uncertain identification when using tests such as CHROMagar Candida, API® Candida, API® ID32C or VITEK® MS, leads to an underestimation of the cases produced by this yeast.</p></div><div><h3>Aims</h3><p>To report the first isolation of <em>C</em>. <em>fabianii</em> in Chile and its identification.</p></div><div><h3>Methods</h3><p>The sequencing of the internal transcribed spacer region (ITS) was performed. Antifungal susceptibility profiles were obtained by means of the broth microdilution technique.</p></div><div><h3>Results</h3><p>The identification was only reached by sequencing the ITS regions, which shows the limited usefulness of the conventional techniques in the identification of some yeast species. A dendrogram shows the phylogenetic relationship of the isolated strain with some other yeast species.</p></div><div><h3>Conclusion</h3><p>In the identification of fastidious microorganisms or microorganisms whose identification is not completely reliable when using classical or even advanced methodologies, such as mass spectrometry, sequencing techniques are essential.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 180-183"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2021.04.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90023490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Super moulds and Scedosporium species","authors":"F. Javier Cabañes","doi":"10.1016/j.riam.2021.05.001","DOIUrl":"10.1016/j.riam.2021.05.001","url":null,"abstract":"","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 157-158"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2021.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39172862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentially expressed proteins in the interaction of Paracoccidioides lutzii with human monocytes","authors":"Flamélia Carla Silva Oliveira ,&nbsp;Wallace Felipe Blohem Pessoa ,&nbsp;Joise Hander Mares ,&nbsp;Herbert Pina Silva Freire ,&nbsp;Ednara Almeida de Souza ,&nbsp;Carlos Priminho Pirovani ,&nbsp;Carla Cristina Romano","doi":"10.1016/j.riam.2020.09.006","DOIUrl":"10.1016/j.riam.2020.09.006","url":null,"abstract":"<div><h3>Background</h3><p>Fungi of the genus <span><em>Paracoccidioides</em></span> are the etiological agents of paracoccidioidomycosis, a highly prevalent mycosis in Latin America. Infection in humans occurs by the inhalation of conidia, which later revert to the form of yeast. In this context, macrophages are positioned as an important line of defense, assisting in the recognition and presentation of antigens, as well as producing reactive oxygen species that inhibit fungal spreading.</p></div><div><h3>Aims</h3><p>The objective of this study was to identify differentially expressed proteins during the interaction between <em>Paracoccidioides lutzii</em> Pb01 strain and human U937 monocytes.</p></div><div><h3>Methods</h3><p>Two-dimensional electrophoresis, combined with mass spectrometry, was used to evaluate the differential proteomic profiles of the fungus <em>P. lutzii</em> (Pb01) interacting with U937 monocytes.</p></div><div><h3>Results</h3><p>It was possible to identify 25 proteins differentially expressed by Pb01 alone and after interacting with U937 monocytes. Most of these proteins are directly associated with fungal metabolism for energy generation, such as glyceraldehyde-3-phosphate dehydrogenase, and intracellular adaptation to monocytes. Antioxidant proteins involved in the response to oxidative stress, such as peroxiredoxin, cytochrome, and peroxidase, were expressed in greater quantity in the interaction with monocytes, suggesting their association with survival mechanisms inside phagocytic cells. We also identified 12 proteins differentially expressed in monocytes before and after the interaction with the fungus; proteins involved in the reorganization of the cytoskeleton, such as vimentin, and proteins involved in the response to oxidative stress, such as glioxalase 1, were identified.</p></div><div><h3>Conclusions</h3><p>The results of this proteomic study of a <em>P. lutzii</em> isolate are novel, mimicking in vitro what occurs in human infections. In addition, the proteins identified may aid to understand fungal–monocyte interactions and the pathogenesis of paracoccidioidomycosis.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 159-167"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39642633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anidulafungin versus fluconazole in the treatment of Candida albicans chorioretinitis","authors":"Celia Ruiz-Arranz ,&nbsp;Eugenio Pérez-Blázquez ,&nbsp;Almudena De Pablo-Cabrera ,&nbsp;Manuel Ferro-Osuna","doi":"10.1016/j.riam.2021.05.002","DOIUrl":"10.1016/j.riam.2021.05.002","url":null,"abstract":"<div><h3>Background</h3><p><span><em>Candida albicans</em></span> chorioretinitis is the most common cause of endogenous fungal endophthalmitis. Echinocandins are recommended as first-line therapy in the treatment of invasive candidiasis (IC), but in clinically stable patients with IC and endophthalmitis caused by <em>Candida</em> species susceptible to azole compounds these are the first-line treatment due to their better intraocular penetration.</p></div><div><h3>Case <em>report</em></h3><p>A 42-year-old woman admitted to hospital for duodenal perforation after gastrointestinal surgery and treated with broad-spectrum antibiotics developed <em>C. albicans</em><span> candidemia<span>. According to protocol, an antifungal treatment with anidulafungin was given. The patient presented no visual symptoms but on routinary ophthalmoscopic examination multiple bilateral chorioretinal lesions were observed. Systemic therapy was changed to fluconazole, with good systemic and ocular results.</span></span></p></div><div><h3>Conclusions</h3><p>Azole compounds are the first-line therapy for endophthalmitis associated with candidemia. However, clinical guidelines often propose echinocandins as the first option for IC. In some cases, <em>C. albicans</em> chorioretinitis will require a change in the systemic treatment to assure better intraocular penetration. According to the current evidence and our own experience, routine funduscopy is not necessary in all IC patients. However, we do recommend fundus examination in patients with visual symptoms or those unable to report them (paediatric patients and patients with an altered level of consciousness), and in those who are being treated with echinocandins in monotherapy.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 188-191"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39718160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kerion Celsi caused by Trichophyton tonsurans with dermatophytid reaction","authors":"Elisabeth Gómez-Moyano ,&nbsp;Ana María Fernández-Sánchez ,&nbsp;Vicente Crespo-Erchiga ,&nbsp;Leandro Martínez-Pilar","doi":"10.1016/j.riam.2020.12.002","DOIUrl":"10.1016/j.riam.2020.12.002","url":null,"abstract":"","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 3","pages":"Pages 151-152"},"PeriodicalIF":1.9,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.12.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25387954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nosemosis and the collapse of beehives","authors":"F. Javier Cabañes","doi":"10.1016/j.riam.2020.03.003","DOIUrl":"10.1016/j.riam.2020.03.003","url":null,"abstract":"","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 3","pages":"Pages 107-108"},"PeriodicalIF":1.9,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.03.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38077108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}