Azole resistance in a clinical isolate of Aspergillus fumigatus from Chile

IF 1.5 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia Pub Date : 2024-01-01 DOI:10.1016/j.riam.2024.04.003

Eduardo Álvarez Duarte , Nicolás Cepeda , Jean Miranda

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引用次数: 0

Abstract

Background

Aspergillus fumigatus is a ubiquitous opportunistic pathogen. This fungus can acquire resistance to azole antifungals due to different mutations in the cyp51A gene. Azole resistance has been observed in several continents and appears to be a globally distributed phenomenon. Specific mutations in cyp51A that lead to azole resistance, such as the TR34/L98H modification, have been reported.

Aims

To evaluate the azole resistance in clinically isolated A. fumigatus strains.

Methods

As a result of our passive surveillance strategy, a total of 23 A. fumigatus isolates from clinical origins were identified through a phylogenetic analysis using the ITS region and β-tubulin gene fragments, and typed with the CSP microsatellite. Azole susceptibility profiles were performed by disk diffusion and microdilution broth methodologies according to CLSI guidelines.

Results

Here we describe, for the first time, the detection of azole-resistant A. fumigatus isolates from clinical origins in Chile with mutations in the cyp51A gene. In addition to the TR34/L98H mutation, one isolate exhibited an F46Y/M172V/E427K-type mutation. Furthermore, microsatellite typing based on cell surface protein (CSP) was performed, showing the t02 (TR34/L98H), t15 (F46Y/M172V/E427K) and t01 (susceptible clinical isolates) genotypes.

Conclusions

Our study demonstrates the presence of mutations related to azole resistance in A. fumigatus strains isolated from clinical samples in Chile. In order to obtain information that may help to tackle the spread of antifungal resistance among A. fumigatus populations, and to ensure the efficacy of future treatments against aspergillosis, a further research is necessary.

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智利烟曲霉临床分离株的唑类抗药性。

背景：烟曲霉是一种无处不在的机会性病原体。由于 cyp51A 基因的不同突变，这种真菌可获得对唑类抗真菌药的抗药性。唑类抗真菌剂的抗药性已在几大洲被观察到，而且似乎是一种全球分布现象。目的：评估临床分离的烟曲霉菌株的唑类抗药性：方法：通过被动监测策略，利用 ITS 区域和 β-管蛋白基因片段进行系统发育分析，确定了 23 株临床分离的烟曲霉菌株，并用 CSP 微卫星进行了分型。根据 CLSI 指南，通过盘式扩散和微量稀释肉汤法进行了唑类药敏性分析：结果：我们在此首次发现了来自智利临床的耐唑烟曲霉分离物，其cyp51A基因发生了突变。除了 TR34/L98H 突变外，其中一个分离株还出现了 F46Y/M172V/E427K 型突变。此外，还进行了基于细胞表面蛋白（CSP）的微卫星分型，显示出 t02（TR34/L98H）、t15（F46Y/M172V/E427K）和 t01（易感临床分离株）基因型：我们的研究表明，从智利临床样本中分离出的烟曲霉菌株中存在与唑类抗性相关的突变。为了获得有助于解决烟曲霉菌抗药性扩散问题的信息，并确保未来治疗曲霉菌病的疗效，有必要开展进一步的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Iberoamericana De Micologia MYCOLOGY-

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

81 days

期刊介绍： Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.