用于诊断念珠菌血症的特异性和非特异性血液培养瓶的实验研究。

IF 1.5 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia Pub Date : 2024-01-01 DOI:10.1016/j.riam.2024.06.001

Leandre Carmem Wilot , Vanice Rodrigues Poester , Cecília Bittencourt Severo , Karine Ortiz Sanchotene , Bruna Muradás Esperon , Mariana Rodrigues Trápaga , David A. Stevens , Melissa Orzechowski Xavier

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引用次数: 0

摘要

背景：念珠菌血症的早期诊断对于患者的正确治疗至关重要：目的：测试不同血液培养瓶对念珠菌菌株生长的功效：我们使用 BD BACTEC™ FX 40 自动血液培养系统比较了 BD BACTEC™ Plus Aerobic/F (Aero) 培养瓶和特定的 BD BACTEC™ Mycosis IC/F Lytic (Myco) 培养瓶的性能，以确定念珠菌菌株的平均检测时间 (TTD)。将六种念珠菌中的每一种接种到血液培养瓶中（最终浓度为 1-5CFUml-1），并在 37°C 温度下培养至自动生长检测：结果：白色念珠菌和光滑念珠菌（Nakaseomyces glabratus）在特异性培养瓶中更早被检测到，而热带念珠菌在非特异性培养瓶中更早被检测到；副丝状念珠菌、Pichia kudriavzevii（克鲁塞念珠菌）和Meyerozyma guilliermondii（吉利蒙念珠菌）在两种培养瓶中的TTD相似：我们的研究表明，在临床实验室中使用这两种瓶子可以更快地做出诊断并迅速开始治疗。

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Experimental study of specific and nonspecific blood culture bottles for the diagnosis of candidemia

Background

Early diagnosis of candidemia is critical for the correct management and treatment of patients.

Aims

To test the efficacy of different blood culture bottles in the growth of Candida strains.

Methods

We compared the performance of BD BACTEC™ Plus Aerobic/F (Aero) culture bottles with the specific BD BACTEC™ Mycosis IC/F Lytic (Myco) culture bottles using the BD BACTEC™ FX 40 automated blood culture system to determine the mean time-to-detection (TTD) in Candida species. One isolate each of six Candida species was inoculated into blood culture bottles (final concentration, 1–5 CFU ml⁻¹) and incubated at 37 °C until automated growth detection.

Results

Candida albicans and Nakaseomyces glabratus (Candida glabrata) were detected earlier in the specific culture bottle, whereas Candida tropicalis was detected earlier in the nonspecific bottle; Candida parapsilosis, Pichia kudriavzevii (Candida krusei), and Meyerozyma guilliermondii (Candida guilliermondii) presented similar TTD in both bottles.

Conclusions

Our study suggests the suitability of using both bottles in clinical laboratories for a faster diagnosis and prompt starting of any treatment.

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来源期刊

Revista Iberoamericana De Micologia MYCOLOGY-

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

81 days

期刊介绍： Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.