Revista Argentina de microbiologia最新文献

{"title":"Detection of Campylobacter spp. and Yersina spp. in chicken burgers: Critical evaluation of CIN medium and characterization of psychrotolerant microbiota in commercial samples from Reus (Tarragona, Spain)","authors":"Marta Sanchis ,&nbsp;Laura Camuña-Pardo ,&nbsp;Laia Martínez Quijada ,&nbsp;Altea Pérez Escobar ,&nbsp;Frederic Gómez-Bertomeu ,&nbsp;Alberto Miguel Stchigel Glikman","doi":"10.1016/j.ram.2025.06.002","DOIUrl":"10.1016/j.ram.2025.06.002","url":null,"abstract":"<div><div>Two of the three most prevalent bacterial genera associated with human diseases caused by meat consumption are <em>Campylobacter</em> (mainly in poultry meat) and <em>Yersinia</em> (in pork meat). As its detection and quantification is not regulated by the European legislation (except for the quantification of <em>Campylobacter</em> in poultry channels), several samples of chicken burgers from different establishments including supermarkets and retail trade outlets in Reus (Spain) were processed to ensure microbiological safety. Microbiological criteria and procedures have been those included in the European standards for <em>Campylobacter</em> (Regulation No. 2017/1495) and <em>Yersinia</em> (ISO 10273:2917). Results showed the absence of <em>Campylobacter</em> spp. in all samples, but high counts (10⁴ to 5<!--> <!-->×<!--> <!-->10⁵ <!-->CFU/g) of typical colonies (“red bull's eye” morphology) on CIN medium compatible with <em>Yersinia</em> spp. The biochemical profile of the strains from the typical colonies, and their subsequent molecular identification using MALDI-TOF MS enabled us to identify <em>Yersinia intermedia</em> in just one sample, and <em>Pseudomonas</em> and <em>Serratia liquefaciens</em> in the remaining ones. These findings call into question the usefulness of CIN medium for detecting <em>Yersinia</em> spp. in food. Moreover, the presence of high counts of psychrotolerant bacteria from the genera <em>Pseudomonas</em> and <em>Serratia</em> highlight the need to improve hygienic conditions in the procedures used to produce meat derivatives.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 391-398"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variación de la microbiota oral facultativa durante períodos de estrés académico","authors":"Victor Ludeña-Meléndez ,&nbsp;Jussara Malo-Castillo ,&nbsp;Paula Ishikawa-Arias ,&nbsp;Harold Jimenez-Alvarez ,&nbsp;Criss Ocampo ,&nbsp;Cristhian Guevara-Coronel ,&nbsp;Leonardo Ledesma Chavarria ,&nbsp;Meilyn Lujan-Benites ,&nbsp;Cristhian Terrones ,&nbsp;Christian Eduardo Vilca Avalos ,&nbsp;Maria Soledad Ayala Ravelo","doi":"10.1016/j.ram.2025.09.005","DOIUrl":"10.1016/j.ram.2025.09.005","url":null,"abstract":"<div><div>Academic stress is a factor that impacts the health of medical students by altering their body's internal environment and its various constituent systems, including the microbiota of the digestive system, particularly that of the oral cavity. Therefore, the objective of this study was to evaluate the variation in the facultative oral microbiota of medical students during periods of perceived academic stress during evaluation periods. This was an observational, analytical study that used mouthwash samples and microbiological colony-forming units counts per ml (CFU/ml) of oral bacterial and fungal microorganisms. Forty-seven participants were included: 59.57% were male, 55.32% had good oral hygiene habits, and 93.62% belonged to a middle or low socioeconomic status. The academic stress score was higher in response to the stressful event (UAE<!--> <!-->=<!--> <!-->62.5<!--> <!-->±<!--> <!-->12.3) and was significantly associated with a higher CFU/ml count <em>(p</em> &lt;0.05). Yeast colonies corresponding to <em>Candida albicans</em>, <em>C. tropicalis, C. glabrata</em>, and <em>Candida</em> spp. were identified on CHROMagar^TM. It is concluded that there is an association between perceived academic stress and socioeconomic status with the variation in the bacterial and fungal oral microbiota, respectively.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 318-326"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity assessment of Bacillus thuringiensis strains for the control of the lesser mealworm beetle Alphitobius diaperinus (Coleoptera: Tenebrionidae)","authors":"Melisa P. Pérez ,&nbsp;Graciela B. Benintende ,&nbsp;Diego H. Sauka","doi":"10.1016/j.ram.2025.03.003","DOIUrl":"10.1016/j.ram.2025.03.003","url":null,"abstract":"<div><div>This study addresses the pervasive challenge of lesser mealworm <em>Alphitobius diaperinus</em> (Coleoptera: Tenebrionidae) infestations in poultry farming. Our aim was to select toxic <em>Bacillus thuringiensis</em> strains against <em>A. diaperinus</em> larvae and determine the fraction(s) within the bacterial cultures harboring the active metabolites responsible for this insecticidal activity. Among the 41 strains evaluated, the Argentine strain INTA Mo4-4 showed the highest toxicity. Bioassays revealed that the main virulence factors reside within the spore-crystal pellet associated with the Cry protein. These results set the basis for the development of a targeted bioinsecticide. Further research is needed to elucidate the underlying mechanisms and validate these strategies in field trials. By harnessing <em>B. thuringiensis</em>-based bioinsecticides, we offer a sustainable solution to mitigate <em>A. diaperinus</em> infestations.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 375-379"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transmission of viral diseases in wild boar using Rutherford's atomic model","authors":"Germán Ernesto Metz,&nbsp;María Gabriela Echeverría","doi":"10.1016/j.ram.2025.11.001","DOIUrl":"10.1016/j.ram.2025.11.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 307-308"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145610448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mosquito-borne pathogens … Now Rickettsias?","authors":"Silvina Goenaga ,&nbsp;Estefania Raquel Boaglio ,&nbsp;Lucia Victoria Martinez ,&nbsp;Juliana Patricia Sanchez","doi":"10.1016/j.ram.2025.03.002","DOIUrl":"10.1016/j.ram.2025.03.002","url":null,"abstract":"<div><div>In the last few decades, the emergence of several infectious diseases has increased. Mosquitoes are the most important vectors due to their role in transmitting several pathogens, such as <em>dengue, chikungunya</em> and <em>Plasmodium</em> spp. Recently, mosquitoes have been suspected of transmitting Rickettsias, another emerging pathogen. However, their role in the transmission of this agent remains unclear. In this study, we report the detection of a <em>Rickettsia</em> spp. in <em>Coquillettidia venezuelensis</em> mosquitoes collected from Corrientes Province, Argentina. The phylogenetic analyses show that this <em>Rickettsia</em> is related to <em>Rickettsia felis</em>, and the <em>ompB</em> sequence shows high homology with <em>Rickettsia tillamookensis.</em> The evidence of the detection of <em>Rickettsia</em> in mosquitoes shows that several infectious diseases could be transmitted by mosquitoes, and the interaction between them is important to investigate. More studies should be done in order to analyze the impact on public health.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 364-370"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The re-emergence of vaccine-preventable diseases: The case of measles","authors":"Andrea Pontoriero ,&nbsp;Maria Laura Minassian","doi":"10.1016/j.ram.2025.07.001","DOIUrl":"10.1016/j.ram.2025.07.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 205-207"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of intestinal microbiota diversity in children with non-organic anorexia","authors":"Wei Lu,&nbsp;Zong-long Li,&nbsp;De-yong Xu,&nbsp;Guo-ping Yu","doi":"10.1016/j.ram.2025.02.001","DOIUrl":"10.1016/j.ram.2025.02.001","url":null,"abstract":"<div><div>The pathogenesis of non-organic anorexia in children is not clear. This study intends to analyze intestinal bacteria to provide a relevant theoretical basis for the clinical rational selection of microecological agents. In the present study, children with non-organic anorexia were included in the anorexia group and normal healthy children in the control group. Stool samples were collected for the bioinformatics analysis after PCR and high-throughput sequencing. The results showed that the Ace, Chao, and Shannon indexes in the anorexia group were higher than those in the control group, while the Simpson index in the control group was lower than in the anorexia group. There were 14 taxa in the anorexia group and 11 taxa in the healthy control group at the phylum level, and 193 taxa in the anorexia group and 180 in the control group at the genus level. The dominant bacteria at the phylum level of the two groups were the same, while there were 16 dominant bacteria taxa in the anorexia group and 17 in the control group at the genus level. The ratio of percentage abundance of <em>Bacteroidetes</em> to that of <em>Firmicutes</em> (the B/F index) in the anorexia group was higher than in the control group. The abundance of <em>Bacteroidetes</em> in the anorexia group was higher than that in the control group, and the abundance of <em>Actinomycetes</em> in the control group was higher than that in the anorexia group. There were significant differences in 14 dominant genera between the two groups at the genus classification level. The LEfSe multilevel species difference analysis showed that at the phylum level, the significant influential bacterial taxa in the anorexia group were <em>Bacteroidetes</em> and <em>Actinobacteria</em> in the control group. At the genus level, the significant influential bacterial taxa in the anorexia group were <em>Bacteroides</em>, <em>Faecalibacterium</em>, and <em>Subdoligranulum</em>, and <em>Bifidobacterium</em>, <em>Blautia</em>, <em>Streptococcus</em>, <em>Lachnoclostridium</em>, and <em>Erysipelatoclostridium</em> in the control group. We conclude that the increase in <em>Bacteroides</em> abundance or in the B/F index and the reduction in <em>Bifidobacterium</em> abundance were related to the pathogenesis of anorexia.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 221-230"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic resistance to infectious diseases: A complementary tool to antimicrobial drugs in livestock?","authors":"Carlos A. Rossetti ,&nbsp;Ursula A. Rossi","doi":"10.1016/j.ram.2025.06.001","DOIUrl":"10.1016/j.ram.2025.06.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 203-204"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reporte de una experiencia de control del virus de la diarrea viral bovina en 2 tambos de Argentina aplicando herramientas de manejo, diagnóstico y vacunación","authors":"Rodrigo Pereyra ,&nbsp;Fernando Martino ,&nbsp;Mariángeles Castillo ,&nbsp;Juan Manuel Sala ,&nbsp;Lucas José Barone ,&nbsp;Claudio Paolazzi ,&nbsp;Alejandra Victoria Capozzo","doi":"10.1016/j.ram.2024.12.001","DOIUrl":"10.1016/j.ram.2024.12.001","url":null,"abstract":"<div><div>Bovine viral diarrhea virus (BVDV) causes significant economic losses in the international livestock industry and in Argentina, where it circulates at high prevalence. Under high prevalence conditions, BVDV infections are controlled through vaccination once persistently infected animals are identified and segregated. This study evaluated the feasibility of controlling BVDV circulation under field conditions by combining diagnosis, management measures, and vaccination in 2 dairy farms in the province of Santa Fe. Commercial ELISAs were used for the detection of the NS3 (P80) protein or antibodies against this protein as well as an RT-nested PCR for the detection of the viral genome, and viral seroneutralization to assess vaccine efficacy. The average seroprevalence of the farms was 58.4%, with a persistently infected animal rate of 2.4%. After segregating the persistently infected animals and vaccinating them with a commercial combined vaccine containing inactivated BVDV, abortion rates significantly decreased (<em>p</em> <!-->&lt;<!--> <!-->0.05) in farm 1 (from 20.5 to 11.6%) and in farm 2 (from 34 to 23.4%) during the second year of the control strategy. Conception rates increased from 29 to 33% in farm 1 during the first year, while in farm 2, the increase was 7 points during the second year. This methodology achieved conditions in which BVDV ceased to circulate, constituting the first controlled report on BVDV management in dairy farms using tools available to producers in Argentina.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 241-250"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wheat straw amendment to an oily sludge pretreated with ammonium persulfate and its impact on the indigenous mycobiota","authors":"Natalia Andrea Di Clemente ,&nbsp;Marina Peluffo ,&nbsp;Ana Carolina Agnello ,&nbsp;Deborah Colman ,&nbsp;Norma Buceta ,&nbsp;María Teresa Del Panno ,&nbsp;Mario Carlos Nazareno Saparrat","doi":"10.1016/j.ram.2025.05.002","DOIUrl":"10.1016/j.ram.2025.05.002","url":null,"abstract":"<div><div>Numerous studies have addressed the dynamics of bacterial communities in response to remediation strategies, while fungal communities, despite their potential as bioremediation agents, remain comparatively understudied. The aim of this work was to evaluate the impact of wheat straw amendment (WSA) on an oily sludge untreated and treated with ammonium persulfate on indigenous mycobiota after 60 days. Culture-independent 18S rRNA amplicon sequencing, microbial enzymatic activity and chemical parameters were analyzed. WSA on a preoxidized oily sludge promoted high total petroleum hydrocarbon (TPH) removal after 60 days, exhibiting the highest laccase activity. Fungal diversity and equitability were also recovered until reaching similar index values to those of the untreated microcosms. However, changes in fungal taxonomical groups were detected, with Eurotiales (93.4%) being replaced by Microascales (57.9%) and Sordariales (32.8%) at the end of the treatment. Our results suggest that inputs of easily assimilable organic matter in oily sludge might accelerate changes and replacement of fungal taxa, also affecting microbial colonization and, consequently, pollutant removal. These findings highlight the relevance of incorporating fungal dynamics into bioremediation strategies as a complementary approach to oily sludge treatment.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 288-297"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}