Revista Argentina de microbiologia最新文献_第4页

La deficiencia en el transporte de N-acetilglucosamina en Lysinibacillus sphaericus ASB13052 afecta el proceso de esporulación e incrementa la actividad hemolítica de su S-layer [N-乙酰葡糖胺转运缺陷会影响孢子的形成过程，并增加莱氏裂殖杆菌 ASB13052 中 S 层蛋白的溶血活性】。］

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-07-01 DOI: 10.1016/j.ram.2024.05.006

Julián Tarsitano , Sabrina Sol Bockor , María Mercedes Palomino , Joaquina Fina Martin , Sandra Mónica Ruzal , Mariana Claudia Allievi

{"title":"La deficiencia en el transporte de N-acetilglucosamina en Lysinibacillus sphaericus ASB13052 afecta el proceso de esporulación e incrementa la actividad hemolítica de su S-layer","authors":"Julián Tarsitano , Sabrina Sol Bockor , María Mercedes Palomino , Joaquina Fina Martin , Sandra Mónica Ruzal , Mariana Claudia Allievi","doi":"10.1016/j.ram.2024.05.006","DOIUrl":"10.1016/j.ram.2024.05.006","url":null,"abstract":"<div><p><em>Lysinibacillus sphaericus</em> is a bacterium that, along with <em>Bacillus thuringiensis</em> var. <em>israelensis</em>, is considered the best biological insecticide for controlling mosquito larvae and an eco-friendly alternative to chemical insecticides. It depends on peptidic molecules such as N-acetylglucosamine to obtain carbon sources and possesses a phosphotransferase system (PTS) for their incorporation. Some strains carry S-layer proteins, whose involvement in metal retention and larvicidal activity against disease-carrying mosquitoes has been demonstrated. Alterations in the amino sugar incorporation system could affect the protein profile and functionality. Strain ASB13052 and the isogenic mutant in the <em>ptsH</em> gene, which is predominant in the PTS signaling pathway, were used in this study. For the first time, the presence of N-glycosylated S-layer proteins was confirmed in both strains, with a variation in their molecular weight pattern depending on the growth phase. In the exponential phase, an S-layer protein greater than 130 kDa was found in the <em>ptsH</em> mutant, which was absent in the wild-type strain. The mutant strain exhibited altered and incomplete low quality sporulation processes. Hemolysis analysis, associated with larvicidal activity, showed that the <em>ptsH</em> mutant has higher lytic efficiency, correlating with the high molecular weight protein. The results allow us to propose the potential effects that arise as a result of the absence of amino sugar transport on hemolytic activity, S-layer isoforms, and the role of N-acetylglucosamine in larvicidal activity.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S032575412400083X/pdfft?md5=7c35b9d4ac7b41a49f9e26ab51490125&pid=1-s2.0-S032575412400083X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142111472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycobacterium tuberculosis Beijing in the State of Nuevo Leon, Mexico. 墨西哥新莱昂州的北京结核分枝杆菌。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-06-27 DOI: 10.1016/j.ram.2023.12.007

Nohemí Catana-Botello, Pola Becerril-Montes, Jorge Castro-Garza, Francisco González-Salazar, Horacio Almanza-Reyes, María De Los Ángeles Del Bosque-Moncayo, Alejandro Morales-Vargas, Víctor Manuel Velázquez-Moreno

{"title":"Mycobacterium tuberculosis Beijing in the State of Nuevo Leon, Mexico.","authors":"Nohemí Catana-Botello, Pola Becerril-Montes, Jorge Castro-Garza, Francisco González-Salazar, Horacio Almanza-Reyes, María De Los Ángeles Del Bosque-Moncayo, Alejandro Morales-Vargas, Víctor Manuel Velázquez-Moreno","doi":"10.1016/j.ram.2023.12.007","DOIUrl":"https://doi.org/10.1016/j.ram.2023.12.007","url":null,"abstract":"<p><p>Tuberculosis remains a serious threat to human health as an infectious disease in Mexico. Data about the genotypes of circulating Mycobacterium tuberculosis isolates (MTB) in the State of Nuevo Leon, Mexico are scarce. We aimed to determine the genotypes of circulating MTB belonging to the Beijing lineage recovered from patients in the State of Nuevo Leon, Mexico. A total of 406 MTB isolates from this state were genotyped using the spoligotyping method and 18-locus MIRU-VNTR. Lineage classification and MTB transmission analysis were performed. Based on the spoligotyping analysis, we found 24 strains belonging to the Beijing genotype that were characterized phylogenetically. The MIRUs showed greater discriminatory power than the standard RFLP-IS6110 method; therefore, the greatest allelic diversity among the Beijing strains was observed with MIRU10, MIRU31, MIRU39, MRU40, and MIRU 26. MVLA analysis showed a profile variation between Beijing and non-Beijing strains. The minimum spanning tree (MST) showed that 79% (19) of the strains are related. All Beijing strains exhibited the deletion of region TbD1, which is a characteristic of modern strains. The application of spoligotyping and MIRU-VNTR-18 methods together proved to be more sensitive, discriminatory, and rapid than the standard method for the epidemiological analysis of Mycobacterium Beijing isolates. This study is one of the first to describe the genomic diversity of M. Beijing in the State of Nuevo Leon, Mexico.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution, seroprevalence and risk factors for bovine brucellosis in Brazil: Official data, systematic review and meta-analysis 巴西牛布氏杆菌病的分布、血清流行率和风险因素：官方数据、系统回顾和荟萃分析。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.08.002

Isis Daniele dos Santos Rocha , Inácio José Clementino , Débora Luise Canuto de Sousa , Clebert José Alves , Carolina de Sousa Américo Batista Santos , Sérgio Santos de Azevedo

{"title":"Distribution, seroprevalence and risk factors for bovine brucellosis in Brazil: Official data, systematic review and meta-analysis","authors":"Isis Daniele dos Santos Rocha , Inácio José Clementino , Débora Luise Canuto de Sousa , Clebert José Alves , Carolina de Sousa Américo Batista Santos , Sérgio Santos de Azevedo","doi":"10.1016/j.ram.2023.08.002","DOIUrl":"10.1016/j.ram.2023.08.002","url":null,"abstract":"<div><p>Bovine brucellosis is an endemic disease in Brazil, and evidence-based assessments of the available literature on its seroprevalence and risk factors are limited. The aim of this study was to systematically review and summarize studies related to seroprevalence and risk factors of bovine brucellosis in the entire Brazil, in addition to comparing published data with the most recent official reports. Articles available in scientific databases and published between October 2006 and October 2021 were evaluated. Forty-five publications were included in the meta-analysis on the seroprevalence of brucellosis and 29 publications in the review on risk factors. The largest number of publications was found for the State of Mato Grosso do Sul (n=4), and the highest and lowest seroprevalences were observed in Acre (11%; 95% CI: 8.0–14.0%) and in the Federal District (0.4%; 95% CI: 0.2–0.7%). The main risk factors were the purchase of animals for breeding, vaccination, the number of heifers (female ≥2 years), the presence of calving paddocks and the occurrence of abortions. The need for new official studies has been suggested to determine the true prevalence of bovine brucellosis in Brazil, supported by the National Program for the Control and Eradication of Animal Brucellosis and Tuberculosis.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123001098/pdfft?md5=e16b23c9849da0007966bfe65aa14673&pid=1-s2.0-S0325754123001098-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139098560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional study of genes involved in the passage from teliospore to hyphae stage in the fungus Thecaphora frezii, the causal agent of peanut smut 花生烟粉虱病原真菌 Thecaphora frezii 从端孢子到菌丝阶段所涉及基因的转录研究。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.10.002

María S. Díaz , Néstor W. Soria , Ana C. Figueroa , Pablo Yang , Esteban H. Badariotti , Valeria R. Alasino , Pablo Vélez , Dante M. Beltramo

{"title":"Transcriptional study of genes involved in the passage from teliospore to hyphae stage in the fungus Thecaphora frezii, the causal agent of peanut smut","authors":"María S. Díaz , Néstor W. Soria , Ana C. Figueroa , Pablo Yang , Esteban H. Badariotti , Valeria R. Alasino , Pablo Vélez , Dante M. Beltramo","doi":"10.1016/j.ram.2023.10.002","DOIUrl":"10.1016/j.ram.2023.10.002","url":null,"abstract":"<div><p>Peanuts (<em>Arachis hypogaea</em> L.) are among the most important leguminous crops in Argentina. During the growing season, they are frequently attacked by fungal diseases, including <em>Thecaphora frezii</em>. The spores of <em>T. frezii</em> are structures that confer resistance to this phytopathogen. The transition from teliospore to hypha is a characteristic process of some fungi, which is essential for completing their life cycle. Using the transcriptomes of teliospores and hyphae of <em>T. frezii</em>, we aimed to identify genes that were differentially expressed during this transition, and we found 134 up-regulated and 66 down-regulated genes, which would participate in different cellular processes such as: (a) cell cycle and DNA processing; (b) cell fate; (c) rescue, defense and cellular virulence; (d) detoxification by CYP450; (e) energy; (f) nutrient interaction and nutritional adaptation; (g) metabolism; (g) proteins with binding functions or cofactor requirements; (h) stress, cell differentiation and biogenesis of cell components; and (i) transport, cell communication and transcription. The identification of genes in <em>T. frezii</em> and their expression levels during different stages of differentiation could contribute to our understanding of the biological mechanisms in this fungus.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000038/pdfft?md5=11d862ad8a5aea638a6c5f4342331d5a&pid=1-s2.0-S0325754124000038-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139712941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbapenemen-resistant Enterobacterales: An issue of global concern 耐碳青霉烯类肠杆菌：全球关注的问题

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2024.05.001

Marcela Nastro

引用次数: 0

Prevalencia y distribución geográfica de las enfermedades de transmisión sexual de los bovinos en la provincia de Formosa, Argentina [阿根廷福尔摩沙省牛性传播疾病的流行率和地理分布]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.07.004

María Nair Viola , Iris Carolina Elías , Marcelo Signorini , Ana Ines Molineri , Ana María Russo , Patricia Andrea Zimmer , Laura Analía Lozina , Juana Noemí Gimenez

{"title":"Prevalencia y distribución geográfica de las enfermedades de transmisión sexual de los bovinos en la provincia de Formosa, Argentina","authors":"María Nair Viola , Iris Carolina Elías , Marcelo Signorini , Ana Ines Molineri , Ana María Russo , Patricia Andrea Zimmer , Laura Analía Lozina , Juana Noemí Gimenez","doi":"10.1016/j.ram.2023.07.004","DOIUrl":"10.1016/j.ram.2023.07.004","url":null,"abstract":"<div><p>Bovine genital campylobacteriosis (BGC) and bovine trichomonosis (BT) are sexually transmitted diseases (STDs) that affect bovine breeding herds, decreasing their reproductive efficiency. The objective of this work was to estimate the prevalence of these diseases and their temporal-spatial distribution in the province of Formosa, Argentina. The cross-sectional study conducted between 2018 and 2021 included a total of 15,571 bulls, inter-herd prevalence being 29.62% and 17.23% for BGC and BT, respectively. The prevalence of positive animals was 2.05% for BGC and 0.43% for BT. The temporal-spatial analysis of BGC showed two distinct spatial groupings, one group had a low risk of contracting the disease (RR = 0.13; <em>p</em> < 0.001; 2018–2021) while the other group had a high risk (RR = 2.84; <em>p</em> < 0.001; 2020–2021). BT had a high-risk group for the disease (RR = 35.24<em>; p</em> < 0.001; 2019). This study shows that STDs are endemic in the region, providing updated and valuable information as a tool for the health management of these diseases.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000627/pdfft?md5=756acbfa3de90a2c6d1cbd1f7ba2b321&pid=1-s2.0-S0325754123000627-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72015171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The microPIXE technique to understand the distribution of heavy metals in arbuscular mycorrhizal symbiosis 利用微PIXE技术了解重金属在假根菌根共生中的分布。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.12.006

M. Statello , R.P. Colombo , E.M. de la Fournière , M.E. Debray , A.M. Godeas , V.A. Silvani

引用次数: 0

Carpogenic germination of Sclerotinia sclerotiorum sclerotia in Brassica oleracea var gemmifera (VN: Brussels sprouts) in North Patagonia, Argentina 阿根廷北巴塔哥尼亚甘蓝菜（Brassica oleracea var gemmifera）（VN：球芽甘蓝）中硬粒菌的鲤鱼发芽。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.12.005

A.G. Scarso , M.C. Sosa , M.J. Ousset , M.C. Lutz

引用次数: 0

In house-development of a rapid immunochromatographic test for the detection of Escherichia coli in urine samples 内部开发用于检测尿液样本中大肠杆菌的快速免疫层析检测法

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.10.001

Paula Da Cunda , Amy Mónaco , María Moreno , María José Gonzalez , Paola Scavone , Luciana Robino

{"title":"In house-development of a rapid immunochromatographic test for the detection of Escherichia coli in urine samples","authors":"Paula Da Cunda , Amy Mónaco , María Moreno , María José Gonzalez , Paola Scavone , Luciana Robino","doi":"10.1016/j.ram.2023.10.001","DOIUrl":"10.1016/j.ram.2023.10.001","url":null,"abstract":"<div><p>Urinary tract infections (UTIs) are a common health concern. Urine culture is the “gold standard” for UTI diagnosis but takes 48h. Rapid methods like dipstick tests are used as point-of-care tests. However, their sensitivity and specificity are variable. In this work, a rapid immunochromatographic test (IT) for detecting <em>Escherichia coli</em> in urine was developed, and its performance was evaluated in urine samples from patients with suspected UTI. The “universal lateral flow assay kit” was employed using an <em>E. coli</em> capture antibody. One hundred and five (105) urine samples were analyzed using the IT, dipstick test, and urine culture. The sensitivity of the IT was 74.5%, specificity 88.9%, positive predictive value (PPV) 86.3%, and negative predictive value (NPV) 78.7%. The combination of the IT with the dipstick test increases sensitivity to 94.1%, specificity to 66.7%, PPV to 72.7%, and NPV to 92.3%. Using the IT for detecting <em>E. coli</em> in urine could be a valuable technique for UTI screening, showing better specificity and diagnostic precision but lower sensitivity than the dipstick test. Based on these results, we propose that the combined use of both screening techniques would allow a rapid and more precise diagnosis of UTI, rationalizing the indication for empirical antibiotics.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000846/pdfft?md5=5dd878bb5bfa171fb4bec9b4b72511e8&pid=1-s2.0-S0325754123000846-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138558474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory action of antibiotics on Kluyveromyces marxianus 抗生素对 Kluyveromyces marxianus 的抑制作用。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.12.004

Rafael Lisandro Althaus, Orlando Guillermo Nagel, Dafna Eluk

{"title":"Inhibitory action of antibiotics on Kluyveromyces marxianus","authors":"Rafael Lisandro Althaus, Orlando Guillermo Nagel, Dafna Eluk","doi":"10.1016/j.ram.2023.12.004","DOIUrl":"10.1016/j.ram.2023.12.004","url":null,"abstract":"<div><p>A bioassay containing <em>Kluyveromyces marxianus</em> in microtiter plates was used to determine the inhibitory action of 28 antibiotics (aminoglycosides, beta-lactams, macrolides, quinolones, tetracyclines and sulfonamides) against this yeast in whey. For this purpose, the dose–response curve for each antibiotic was constructed using 16 replicates of 12 different concentrations of the antibiotic. The plates were incubated at 40°C until the negative samples exhibited their indicator (5–7h). Subsequently, the absorbances of the yeast cells in each plate were measured by the turbidimetric method (<em>λ</em>   <!-->nm) and the logistic regression model was applied. The concentrations causing 10% (IC10) and 50% (IC50) of growth inhibition of the yeast were calculated. The results allowed to conclude that whey contaminated with cephalosporins, quinolones and tetracyclines at levels close to the Maximum Residue Limits inhibits the growth of <em>K. marxianus</em>. Therefore, previous inactivation treatments should be implemented in order to re-use this contaminated whey by fermentation with <em>K. marxianus</em>.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000063/pdfft?md5=8cbf2798c3ebf9d3e8961caef23ab7af&pid=1-s2.0-S0325754124000063-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0