Revista Argentina de microbiologia最新文献_第4页

Epidemiology of colistin resistance mediated by mcr-1 in carbapenemase-producing Enterobacterales in Ecuador in three periods from 2016 to 2022

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-01-01 DOI: 10.1016/j.ram.2024.12.007

Claudia Soria-Segarra , Carmen Soria-Segarra , José Gutierrez-Fernandez

{"title":"Epidemiology of colistin resistance mediated by mcr-1 in carbapenemase-producing Enterobacterales in Ecuador in three periods from 2016 to 2022","authors":"Claudia Soria-Segarra , Carmen Soria-Segarra , José Gutierrez-Fernandez","doi":"10.1016/j.ram.2024.12.007","DOIUrl":"10.1016/j.ram.2024.12.007","url":null,"abstract":"<div><div>Carbapenemase-producing Enterobacterales (CPE) have increased in the last decade. In low-income countries, colistin is considered a last resort antimicrobial to treat CPE infections, whose most worrisome mechanism of resistance is MCR-1 production. This study aims to understand the epidemiology of colistin resistance in CPE in the region, through the surveillance of the mcr-1 gene in CPE isolates in Ecuador. A total of 361 CPE isolates were collected across three periods, from 2016 to 2022. Colistin resistance was assessed using the broth microdilution method and the most frequent carbapenemase-encoding genes and the mcr-1 gene were studied. Colistin resistance rate increased from 3.76% to 23.74% during the study period. The mcr-1 gene was not identified in any of the isolates studied and Klebsiella pneumoniae blaKPC was the most prevalent microorganism (n=322; 89.20%). In conclusion, colistin resistance increased in CPE in Ecuador and was not mediated by the mcr-1 gene. Our results highlight the need to closely monitor national politics on antimicrobial resistance under the One Health Approach.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 33-38"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Identification and antibiotic susceptibility of Aeromonas spp. in a University Hospital in the city of Buenos Aires]. [布宜诺斯艾利斯某大学医院气单胞菌的鉴定及抗生素敏感性]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-12-21 DOI: 10.1016/j.ram.2024.11.001

Eduardo Montalvo, Florencia Veiga, Hernán Rodríguez, German Traglia, Carlos Vay, Marisa Almuzara

{"title":"[Identification and antibiotic susceptibility of Aeromonas spp. in a University Hospital in the city of Buenos Aires].","authors":"Eduardo Montalvo, Florencia Veiga, Hernán Rodríguez, German Traglia, Carlos Vay, Marisa Almuzara","doi":"10.1016/j.ram.2024.11.001","DOIUrl":"https://doi.org/10.1016/j.ram.2024.11.001","url":null,"abstract":"Aeromonas spp. are opportunistic pathogens that cause both intra- and extraintestinal infections. The objective of this work was the phenotypic and genotypic characterization of a collection of Aeromonas strains, in addition to determining their sensitivity to different antimicrobials. Thirty seven isolates were analyzed. 54% were of intra-abdominal origin, 22% from skin and soft tissues, 19% from the bloodstream, among other less frequent sites. By amplification and sequencing of the gyrB gene, which was considered the reference method, the following were identified: 37,8% as species of the Aeromonas hydrophila complex, 32,4% as species of the Aeromonas veronii complex, and 29,7% as species of the complex Aeromonas caviae. Identification by traditional biochemical tests presented a better correlation with molecular identification than mass spectrometry (MALDI TOF MS). Regarding antibiotic sensitivity, cefotaxime, ceftazidime, cefepime, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, ciprofloxacin, amikacin, gentamicin and nitrofurantoin showed activity on more than 80.0% of the isolates tested. The sensitivity and specificity of the phenotypic methods to determine the presence of carbapenemases in relation to the detection of the cphAgene, the reference method, was 60,9% and 100%, respectively, for the colorimetric assay (Blue Carba), and of 91,3% and 50,0% respectively, for the modified Hodge test. The overall resistance to colistin was 32,4%. The automated method showed a very higher error (VME) of 16,2%, while the rapid colorimetric screening method (CRTc) showed an excellent correlation (VME 0%) with the reference method, broth microdilution.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro and in vivo study of the antifungal activity of extracellular products of cyanobacterium Neowestiellopsis persica strain A1387 against Fusarium wilt disease of cucumber. 蓝藻新芽胞外产物A1387对黄瓜枯萎病的体外和体内抑菌活性研究

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-12-19 DOI: 10.1016/j.ram.2024.10.011

Bahareh Nowruzi, Hassan Beiranvand

{"title":"In vitro and in vivo study of the antifungal activity of extracellular products of cyanobacterium Neowestiellopsis persica strain A1387 against Fusarium wilt disease of cucumber.","authors":"Bahareh Nowruzi, Hassan Beiranvand","doi":"10.1016/j.ram.2024.10.011","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.011","url":null,"abstract":"Fusarium wilt of cucumber, caused by the fungus Fusarium oxysporum, is a major plant disease that causes significant economic losses. The extensive use of chemical fungicides for its control poses environmental and health risks. Due to growing concerns about the detrimental effects of chemical fungicides, finding safe and effective bio-based alternatives for plant disease control is of high importance. In this study, the potential of Neowestiellopsis persica A1387 cyanobacterial metabolites as a promising substitute for chemical fungicides in controlling this disease was investigated. The antifungal activity of N. persica A1387 cyanobacterial exopolysaccharide (EPS) extract was evaluated against F. oxysporum under in vitro and in vivo conditions. Cucumber plants infected with the fungus were treated with cyanobacterial EPS extract and then assessed for disease severity, antioxidant enzyme activity, and growth parameters. Both biomass and EPS extracts of N. persica A1387 cyanobacteria significantly increased the diameter of the F. oxysporum growth inhibition zone under in vitro conditions. Treatment with cyanobacterial EPS extract resulted in increased dry and fresh weight of stem and roots, and a significant reduction in disease severity and percentage in F. oxysporum-infected plants. Peroxidase, superoxide dismutase (SOD), and catalase enzyme activities in fungus-infected plants treated with cyanobacterial EPS extract were significantly lower on day 42 of infection compared to untreated and infected control plants. These findings demonstrate the potential of N. persica A1387 cyanobacterial extracts as natural and safe alternatives to chemical fungicides for controlling cucumber Fusarium wilt disease.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Broaden properties of ambroxol hydrochloride as an antibiofilm compound. 盐酸氨溴索抗生物膜化合物的拓宽性质。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-12-17 DOI: 10.1016/j.ram.2024.10.010

María J González, Michela Lain, Victoria Iribarnegaray, Luciana Robino, Paola Scavone

{"title":"Broaden properties of ambroxol hydrochloride as an antibiofilm compound.","authors":"María J González, Michela Lain, Victoria Iribarnegaray, Luciana Robino, Paola Scavone","doi":"10.1016/j.ram.2024.10.010","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.010","url":null,"abstract":"Biofilm-associated microorganisms can cause many infections and are an important cause of resistance to several antimicrobials. The antibiotic crisis has led to a pressing need for new therapeutic tools. Ambroxol is frequently used as a mucolytic agent in respiratory diseases with increased mucus production. In addition, a wide range of properties has been described, including the effect on biofilms. In this work, we evaluate the anti-biofilm effect of ambroxol on four strains with clinical relevance: Proteus mirabilis, Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. In vitro, biofilm formation was assessed using the crystal violet quantification technique in microplate and glass coverslip. The inhibition of biofilm formation was evaluated by adding ambroxol at the initial time. Ambroxol hydrochloride was evaluated over the preformed biofilm and live/dead bacteria were quantified. The effect of ambroxol in the ethidium bromide efflux assay and the relative expression of the five major P. mirabilis efflux pump family genes were analyzed. Ambroxol inhibited biofilm formation in all the bacteria tested. Moreover, ambroxol significantly reduces both biofilm biomass and viable bacteria. Ambroxol was able to affect P. mirabilis efflux pumps depending on the concentration used and induced the overexpression of several efflux pump genes. In summary, ambroxol kills planktonic cells, reduce biofilm biomass as it increases cell death, and affect the expression of efflux pumps. Furthermore, it presents a viable alternative for the treatment of biofilm infection alone or in combination with antibiotic therapy.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial richness assessment in water and sediments in the northern coast of the Yucatan Peninsula. 尤卡坦半岛北部海岸水和沉积物中的细菌丰富度评估。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-12-05 DOI: 10.1016/j.ram.2024.10.009

Mariela Beatriz Reyes-Sosa, Raúl Valle-Gough, María Del Carmen Ponce-Caballero, María Leticia Arena-Ortiz

{"title":"Bacterial richness assessment in water and sediments in the northern coast of the Yucatan Peninsula.","authors":"Mariela Beatriz Reyes-Sosa, Raúl Valle-Gough, María Del Carmen Ponce-Caballero, María Leticia Arena-Ortiz","doi":"10.1016/j.ram.2024.10.009","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.009","url":null,"abstract":"Given the importance of the coastal environments and the multiple ecological services that they provide, it is important to explore and understand the interactions that occur within them. The microbiome is a key factor for the understanding of the dynamics of these fragile sites. A metagenomic study based on the profiling of the 16S ribosomal gene was carried out in order to assess the bacterial diversity present in the northern coastal zone of the Yucatan Peninsula. The results showed that water and sediment samples share some similarities regarding the bacterial genera found, only differing in the quantitative part. Through a PCO (principal coordinates) analysis clear differences between sediment and water samples could be observed. The highest relative diversity was found in wetland and lagoon sediment samples, respectively. It was observed that 3-8% of the total sequence reads belonged to opportunistic genera such as: Vibrio in the sea samples and Capnocytophaga in the other environments. Salinity and pH were the factors which contributed the most to the differences among the communities in the various environments in the coastal zone. There is an important similarity in the sediments across the different environments within the studied coastal zone. The data presented herein contribute to setting a baseline for research in the coastal region of the Yucatan Peninsula.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of stool molecular panel in hospitalized patients with diarrhea. Experience in a tertiary care center. 粪便分子板在住院腹泻患者中的应用。有三级医疗中心工作经验。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-12-05 DOI: 10.1016/j.ram.2024.10.005

Nicolás Cimmino, Maria Josefina Etchevers, Astrid Smud, Patricia Yolleth Andrade Pino, Marisa Del Lujan Sanchez, Maria Laura Gonzalez, Maria Victoria Acosta, Gissel Zuñiga, Karina Alejandra Perez, Juana Freggiaro, Valeria Rosa Alexander, Maria Florencia Veiga, Mariangeles Visus, Mariano Martin Marcolongo

{"title":"Use of stool molecular panel in hospitalized patients with diarrhea. Experience in a tertiary care center.","authors":"Nicolás Cimmino, Maria Josefina Etchevers, Astrid Smud, Patricia Yolleth Andrade Pino, Marisa Del Lujan Sanchez, Maria Laura Gonzalez, Maria Victoria Acosta, Gissel Zuñiga, Karina Alejandra Perez, Juana Freggiaro, Valeria Rosa Alexander, Maria Florencia Veiga, Mariangeles Visus, Mariano Martin Marcolongo","doi":"10.1016/j.ram.2024.10.005","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.005","url":null,"abstract":"Infectious diarrhea is a common health issue that affects a large number of individuals each year. It causes significant morbidity and mortality, greatly impacting healthcare system costs. Rapid detection of the causative organism and timely treatment alters the management and outcome of the condition. Molecular panels in stool allow to analyze a wide range of pathogens quickly and easily. For this study, a cross-sectional cohort analysis with a retrospective analysis of adult patients hospitalized with diarrhea and negative conventional stool bacteriological studies was conducted. Data obtained from the use of molecular panels in stool and the role of endoscopy in the diagnostic pathway were analyzed. A positivity rate of 52% (n=41) out of a total of 79 samples was reported. The test contributed to a change in therapeutic approach in 58% (n=46) of the patients. Among the patients with a negative molecular panel, 39.5% underwent further evaluation with colonoscopy involving biopsies, resulting in a diagnostic yield of 87%. Based on these results, we can conclude that molecular techniques contribute to the diagnosis and change in therapeutic approach in hospitalized patients with diarrhea.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Risk factors associated with the presence of brucellosis in mixed establishments (caprine and bovine) in the province of Formosa, Argentina]. [阿根廷福莫萨省混合饲养场（山羊和牛）出现布鲁氏菌病的相关风险因素]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-11-20 DOI: 10.1016/j.ram.2024.10.003

Iris Carolina Elías, María Nair Viola, Ana Maria Russo, Marcelo Signorini Porchietto

{"title":"[Risk factors associated with the presence of brucellosis in mixed establishments (caprine and bovine) in the province of Formosa, Argentina].","authors":"Iris Carolina Elías, María Nair Viola, Ana Maria Russo, Marcelo Signorini Porchietto","doi":"10.1016/j.ram.2024.10.003","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.003","url":null,"abstract":"Brucellosis is a zoonotic disease with a worldwide distribution that causes significant productive losses and negative impacts on public health. The aim of this work was to identify the risk factors associated with the presence of brucellosis in mixed farms (cattle/goats) in the central-western region of the province of Formosa. Sixty seven mixed farms with 7,855 animals (2,943 cattle and 4,912 goats) were randomly sampled during 2022/2023. The samples were serologically analyzed (BPA + FPA) to determine which animals were positive. Likewise, in each farm, a confidential survey was carried out to evaluate potential associated variables. The statistical analysis was performed using Generalized Linear Mixed Models (GLMM) with binomial distribution, logarithmic link function, and farm as a random variable. The estimated prevalence of brucellosis in mixed farms was 9%, while the joint prevalence per animal (cattle and goats) was 2.75%. The prevalence of brucellosis in cattle and goats was explained by the presence of animals with reproductive signs (odds ratio, OR=40), the occurrence of abortions in the last term of gestation and the birth of weak offspring (OR=5.3), incorrect treatment of abortions (OR=8), the introduction of animals from other farms (OR=5.9), and not having a negative brucellosis certificate (OR=9.6). The management practices conducted in each farm such as preventive measures, sanitation practices for the pens, and prevention measures for the workers, are important for the control of brucellosis.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Atypical non-Typhi Salmonella enterica bacteremia, lactose-positive, lysine decarboxylase-negative]. [非典型非Typhi肠炎沙门氏菌菌血症，乳糖阳性，赖氨酸脱羧酶阴性]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-11-16 DOI: 10.1016/j.ram.2024.09.007

Susana D García, María I Caffer, Marisa N Almuzara, Ángela M R Famiglietti, Cristina Myburg, María R Viñas, Jorge Risso, Carlos A Vay

引用次数: 0

Detection and characterization of highly pathogenic avian influenza A (H5N1) clade 2.3.4.4b virus circulating in Argentina in 2023. 2023 年在阿根廷流行的高致病性甲型禽流感（H5N1）2.3.4.4b 支系病毒的检测和特征描述。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-11-11 DOI: 10.1016/j.ram.2024.08.002

María Carolina Artuso, Vanina Daniela Marchione, Estefanía Benedetti, Paula Bonastre, Ana María Alvarez, Luana Piccini, Angeles Ponde, Evelyn Barrios Benito, Marcos Fabeiro, Karen Waisman, Luciano Coppola, Tomás Poklepovich, Ariana Chamorro, Martín Avaro, Diego Ariel Riva, Andrea Pontoriero, María Eugenia Ferrer, Andrea Marcos, Lorena Dassa, Daniel Caria, Ximena Melon, Rodrigo Emmanuel Balzano Parodi, Ana María Nicola

{"title":"Detection and characterization of highly pathogenic avian influenza A (H5N1) clade 2.3.4.4b virus circulating in Argentina in 2023.","authors":"María Carolina Artuso, Vanina Daniela Marchione, Estefanía Benedetti, Paula Bonastre, Ana María Alvarez, Luana Piccini, Angeles Ponde, Evelyn Barrios Benito, Marcos Fabeiro, Karen Waisman, Luciano Coppola, Tomás Poklepovich, Ariana Chamorro, Martín Avaro, Diego Ariel Riva, Andrea Pontoriero, María Eugenia Ferrer, Andrea Marcos, Lorena Dassa, Daniel Caria, Ximena Melon, Rodrigo Emmanuel Balzano Parodi, Ana María Nicola","doi":"10.1016/j.ram.2024.08.002","DOIUrl":"https://doi.org/10.1016/j.ram.2024.08.002","url":null,"abstract":"In 2021, avian influenza A (H5N1) clade 2.3.4.4b virus spread to North America and then to Central and South America in October 2022, extending from Colombia to Chile in three months. During 2023, several countries, mostly in the Americas, reported outbreaks in poultry, wild birds and mammals, as well as the emergence of two cases in humans (one in Ecuador in January and one in Chile in March). As of September 20th, 2023, 17 countries in the Americas Region have recorded cases of A (H5N1) in birds and mammals. On February 14th, 2023, Argentina confirmed the first case of avian influenza in wild birds, which was later detected in backyard and commercial poultry, and in the South-American sea lion (Otaria flavescens) in Tierra del Fuego, in the south of the country. So far, 21 suspected cases have been recorded in humans; however, all of them tested negative for Influenza A virus. Hemagglutinin sequence data of animal viruses analyzed in this report showed that Argentinian viruses clustered together with those isolated in other countries of the region. Epidemiological data suggested the possibility of multiple simultaneous entries of the avian virus, highlighting the role of migratory avian populations in the introduction and dissemination of the disease in Argentina. Continued comprehensive surveillance of these viruses in animals and people worldwide, along with ongoing preparedness efforts, are critical to determine the public health risk.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential use of arginine kinase from the brown tick Rhipicephalus sanguineus as a biomarker for vector exposure in the surveillance of Rocky Mountain spotted fever 将棕色蜱 Rhipicephalus sanguineus 的精氨酸激酶用作监测落基山斑疹热的病媒接触生物标志物的可能性。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.08.001

Ana C. Gomez-Yanes , Karina D. Garcia-Orozco , Veronica Vazquez-Villarce , Elena N. Moreno-Cordova , Veronica Mata-Haro , Marcia Leyva-Gastelum , Adan Valenzuela-Castillo , Alonso A. Lopez-Zavala , Ana M. Calderon de la Barca , Jesus G. Valenzuela , Rogerio R. Sotelo-Mundo

{"title":"The potential use of arginine kinase from the brown tick Rhipicephalus sanguineus as a biomarker for vector exposure in the surveillance of Rocky Mountain spotted fever","authors":"Ana C. Gomez-Yanes , Karina D. Garcia-Orozco , Veronica Vazquez-Villarce , Elena N. Moreno-Cordova , Veronica Mata-Haro , Marcia Leyva-Gastelum , Adan Valenzuela-Castillo , Alonso A. Lopez-Zavala , Ana M. Calderon de la Barca , Jesus G. Valenzuela , Rogerio R. Sotelo-Mundo","doi":"10.1016/j.ram.2024.08.001","DOIUrl":"10.1016/j.ram.2024.08.001","url":null,"abstract":"<div><div>The brown dog tick (Rhipicephalus sanguineus) is the vector of Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever (RMSF) in Northern Mexico and Southwestern United States. The immune response to a tick protein in the sera of humans or animals may reveal the zones with a high propensity to acquire RMSF, and vector control strategies may be focused on these zones. Arginine kinase (AK) is a highly antigenic invertebrate protein that may serve as a marker for tick exposure. We used R. sanguineus recombinant AK in an indirect ELISA assay with RMSF-positive patient sera. The response to AK was significantly higher against the sera of RMSF patients than the control sera from healthy participants without contact with dogs. To validate the antigenicity of tick AK, we mutated one predicted conformational epitope to alanine residues, which reduced the recognition by RMSF patients’ immunoglobulins. This preliminary result opens a perspective towards the development of a complimentary technique based on RsAK as an antigen biomarker for vector serological surveillance for Rickettsia RMSF prevention.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 364-367"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0