Revista Argentina de microbiologia最新文献_第3页

Report of a novel pathogenic orthohantavirus in Tucuman, Argentina. 阿根廷图库曼一种新型致病性正汉坦病毒报告。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-03-04 DOI: 10.1016/j.ram.2025.01.001

Julia Brignone, Carina Sen, María Laura Martin, Natalia Fernandez, Gabriela Delgado, Anabel Sinchi, Yael Nazar, María Cecilia Monzani, María Laura Cisneros, Patricia Clua, Rocío Coronel, Jorge García

{"title":"Report of a novel pathogenic orthohantavirus in Tucuman, Argentina.","authors":"Julia Brignone, Carina Sen, María Laura Martin, Natalia Fernandez, Gabriela Delgado, Anabel Sinchi, Yael Nazar, María Cecilia Monzani, María Laura Cisneros, Patricia Clua, Rocío Coronel, Jorge García","doi":"10.1016/j.ram.2025.01.001","DOIUrl":"https://doi.org/10.1016/j.ram.2025.01.001","url":null,"abstract":"The confirmation of two cases of hantavirus pulmonary syndrome (HPS) in the city of San Miguel de Tucuman, province of Tucuman, is described in the present study. In 2021, the diagnosis of two patients with HPS with no history of travel outside the province was confirmed. The infection was determined by serology in both cases. Case 1 had an unfavorable outcome, was confirmed by the detection of specific IgM antibodies for orthohantavirus and the identification in a serum sample from three days of evolution of a viral genotype, which was previously unreported to be in circulation in Argentina. In Case 2, specific IgM and IgG type antibodies were detected in two serum samples, with no amplification of the viral genome. Due to the recreational habits of the patients, it is possible to infer exposure to infected rodent fluids during outdoor activities in their areas of residence. In conclusion, the circulation of a new viral genotype of orthohantavirus in Argentina is confirmed.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro activity of ceftobiprole against Staphylococcus aureus: Current experience. 头孢替比罗对金黄色葡萄球菌的体外活性：目前的经验。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-03-04 DOI: 10.1016/j.ram.2024.12.011

Manuel Paz-Infanzon, Adrian Camacho-Ortiz, Eduardo Perez-Alba, Laura Nuzzolo-Shihadeh, Rayo Morfin-Otero, Paola Bocanegra-Ibarias

引用次数: 0

Clinical and demographic characteristics of spondylodiscitis in a Mexican population: A retrospective study. 墨西哥人群脊柱炎的临床和人口学特征：一项回顾性研究。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-02-25 DOI: 10.1016/j.ram.2025.01.002

Mario Alberto Dueñas-Espinosa, Julio César López-Valdés, Daniel Alejandro Vega-Moreno, María Fernanda Guzmán-Del Río, Rafael Sánchez-Mata, María Elena Córdoba-Mosqueda, Laura Mestre-Orozco, Erick Alberto Castañeda-Ramírez, Alejandro Jacob Madrid-Sánchez, Ulises García-González

{"title":"Clinical and demographic characteristics of spondylodiscitis in a Mexican population: A retrospective study.","authors":"Mario Alberto Dueñas-Espinosa, Julio César López-Valdés, Daniel Alejandro Vega-Moreno, María Fernanda Guzmán-Del Río, Rafael Sánchez-Mata, María Elena Córdoba-Mosqueda, Laura Mestre-Orozco, Erick Alberto Castañeda-Ramírez, Alejandro Jacob Madrid-Sánchez, Ulises García-González","doi":"10.1016/j.ram.2025.01.002","DOIUrl":"https://doi.org/10.1016/j.ram.2025.01.002","url":null,"abstract":"The aim of this study was to identify the clinical and demographic characteristics of patients with a confirmed diagnosis of spondylodiscitis through microbiological cultures. A descriptive, observational, and retrospective study was conducted. Patients were included based on clinical and radiological evidence of vertebral infection, unspecified discitis, and/or positive microbiological cultures consistent with spondylodiscitis. For the comparison between men and women, the Student's t-test and odds ratio were employed. The Chi-square test was used to examine correlations between affected spinal levels, isolated microorganisms, and associated comorbidities. A total of 86 cases of discitis were identified, 65% of which involved male patients. The mean age was 59.0±11.5 years (range: 38-83), and the average body mass index (BMI) was 28±4.05kg/m2. Primary discitis predominated in 68% of cases, mainly at the thoracic level. Seventeen patients presented with spondylodiscitis not associated with chronic degenerative diseases. The most frequently isolated microorganisms were Staphylococcus aureus (28 cases) and Escherichia coli (21 cases). In 16 cases, intracellular pathogens such as Mycobacterium tuberculosis and Brucella spp. were identified, leading to an average hospital stay of 30 days. Spondylodiscitis is a serious complication, and this study highlights differences from previously published data, particularly in terms of the microorganisms involved and the demographic profile of the population.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colistin-resistant Escherichia coli mediated by the mcr-1 gene from pigs in northeastern Argentina. 阿根廷东北部猪的mcr-1基因介导的耐粘菌素大肠杆菌。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-02-20 DOI: 10.1016/j.ram.2024.12.013

Juan Leandro Pellegrini, María de Los Ángeles González, Liliana Silvina Lösch, Luis Antonio Merino, José Alejandro Di Conza

{"title":"Colistin-resistant Escherichia coli mediated by the mcr-1 gene from pigs in northeastern Argentina.","authors":"Juan Leandro Pellegrini, María de Los Ángeles González, Liliana Silvina Lösch, Luis Antonio Merino, José Alejandro Di Conza","doi":"10.1016/j.ram.2024.12.013","DOIUrl":"https://doi.org/10.1016/j.ram.2024.12.013","url":null,"abstract":"The emergence and spread of multidrug-resistant Escherichia coli carrying mcr-1 is recognized as a threat to public health. The aim of this study was to determine the prevalence of the mcr-1 gene in colistin-resistant E. coli isolates from commercial pig farms in Chaco, Argentina from 2020 to 2021. A total of 140 rectal swab samples were collected from pigs in six different pig production farms. Antimicrobial susceptibility was determined by broth microdilution. mcr-1 to mcr-5 genes were identified by multiplex PCR and clonality was assessed by ERIC and REP-PCR. The prevalence of mcr-1 was 16.4% and mcr-2, mcr-3, mcr-4 and mcr-5 genes were not detected. Colistin MIC values showed a bimodal distribution with a MIC50, MIC90 and a range of 4, 8 and 4-8μg/ml, respectively. The resistance profile to other antimicrobials was: ampicillin, 87% (20); ampicillin-sulbactam, 47.8% (11); amoxicillin-clavulanic, 13% (3); chloramphenicol, 82.6% (19); ciprofloxacin, 60.9% (14); minocycline, 26.1% (5) and trimethoprim/sulfamethoxazole, 43.5% (10). Eighty-seven percent (87%) of the strains were categorized as MDR and 12 phenotypic resistance patterns with different clonality profiles were observed. A high prevalence of mcr-1 is demonstrated in colistin-free pig farms from Chaco, Argentina. The mcr-1 positive E. coli isolates showed an alarming level of multidrug resistance and high clonal diversity. It is necessary to continuously monitor the presence of the mcr-1 gene not only in pig production, but also in humans and the environment.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of canine protothecosis caused by Prototheca wickerhamii in Argentina. Brief literature review. 阿根廷首次报道犬科原苔虫引起的犬科原苔病。简要的文献综述。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-02-15 DOI: 10.1016/j.ram.2024.12.010

Silvana Ramadán, Lucía Bulacio, Hernán Dalmaso, Graciela Sepúlveda, Maximiliano Sortino, Fabián Fay, Claudia Misto, María Fernanda Salvador, Alejandro Etchecopaz, María Luján Cuestas

{"title":"First report of canine protothecosis caused by Prototheca wickerhamii in Argentina. Brief literature review.","authors":"Silvana Ramadán, Lucía Bulacio, Hernán Dalmaso, Graciela Sepúlveda, Maximiliano Sortino, Fabián Fay, Claudia Misto, María Fernanda Salvador, Alejandro Etchecopaz, María Luján Cuestas","doi":"10.1016/j.ram.2024.12.010","DOIUrl":"https://doi.org/10.1016/j.ram.2024.12.010","url":null,"abstract":"Protothecosis is an infectious disease caused by microalgae of the genus Prototheca. Prototheca can be found in soil and water and transiently colonize animals. Cutaneous protothecosis can involve not only the skin but also the underlying subcutaneous tissue and lymph nodes. This can lead to clinical signs and a microscopic tissue image that closely resembles a fungal infection. Treatment typically involves antifungal medications. We report the first case of fatal disseminated protothecosis caused by Prototheca wickerhamii in the city of Rosario, Argentina in a 5-year-old female poodle dog. The dog exhibited ocular signs of uveitis and lymphadenitis. To reach a clinical and etiological diagnosis, imaging studies, routine laboratory tests and serological tests were performed. A mycological analysis was conducted on the material obtained by puncturing three lymph nodes. Additionally, morphological, metabolic, and molecular analyses were performed. Antifungal susceptibility testing was also conducted using broth microdilution and diffusion methods. Phenotypic, metabolic, and sequencing techniques identified the isolated organism as P. wickerhamii. This isolate displayed susceptibility to amphotericin, variable susceptibility to itraconazole and voriconazole, and resistance to fluconazole and caspofungin. The frequent presence of pets in our homes highlights the need for a more comprehensive diagnostic approach. This is important because, from a public health perspective, dogs could serve as indicators of algal presence in the environments they frequently share with humans.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Do psychrotolerant Patagonian soil yeasts produce 3-indole acetic acid? 耐寒的巴塔哥尼亚土壤酵母会产生3-吲哚乙酸吗？

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-02-10 DOI: 10.1016/j.ram.2024.12.004

María Cecilia Mestre, Mariana E Langenheim, María Elena Severino, Sonia Fontenla

引用次数: 0

Diversity of hypervirulent Klebsiella pneumoniae clones causing cryptogenic liver abscesses and metastatic complications in Argentina. 阿根廷引起隐源性肝脓肿和转移性并发症的高毒力肺炎克雷伯菌克隆的多样性

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-02-07 DOI: 10.1016/j.ram.2024.11.006

Esteban C Nannini, Matías Lahitte, Pablo Scapellato, Corina Nemirosvky, Marcelo Zylberman, Andrea Vila, Viviana Rodríguez, Roman Zucchi, Analia Mykietiuk, Valeria David, Adriana Limansky, Patricia Marchiaro, Mariángel Rinaudo

{"title":"Diversity of hypervirulent Klebsiella pneumoniae clones causing cryptogenic liver abscesses and metastatic complications in Argentina.","authors":"Esteban C Nannini, Matías Lahitte, Pablo Scapellato, Corina Nemirosvky, Marcelo Zylberman, Andrea Vila, Viviana Rodríguez, Roman Zucchi, Analia Mykietiuk, Valeria David, Adriana Limansky, Patricia Marchiaro, Mariángel Rinaudo","doi":"10.1016/j.ram.2024.11.006","DOIUrl":"https://doi.org/10.1016/j.ram.2024.11.006","url":null,"abstract":"Cryptogenic liver abscesses (CLA) caused by hypervirulent Klebsiella pneumoniae (hvKP) strains are emerging in Western countries. The aim of the study was to describe the clinical characteristics of patients from Argentina with hvKP-related CLA as well as the molecular analysis of isolated strains. A retrospective chart review of 15 patients hospitalized in 8 hospitals of Argentina between October 2015 and November 2018 was performed. PCR assays for genes associated with capsular and multilocus sequence typing (MLST) determination and virulence factors were conducted in 8 hvKP isolates from these patients. We found that the mean age of patients was 60 years, 73% of them were men and 40% suffered from diabetes. Bacteremia was detected in 60% of them and 73% had ≥1 metastatic foci of infection. There were no in-hospital deaths, but two patients with endophthalmitis required eye enucleation. Of the 8 studied isolates, 4 belonged to K1 and 4 to K2 serotypes, with the rpmA and iroB genes being present in all of them, and isolates 7 and 5 also harboring the iucA and the rmpA2 genes, respectively. MSLT analysis showed that most of the K1 serotypes belonged to ST23 while a diverse MLST pattern was observed among the K2 strains. In addition, the four hvKP strains associated with metastatic complications and belonging to three distinct sequence types, exhibited the rpmA, iroB and iuc virulence genes. We were able to demonstrate important morbidity associated with this syndrome, a significant diversity in the hvKP clones causing CLA in Argentina, and the potential utility of the rpmA and iroB genes as predictors of virulence.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of DNA extraction method in gut fungal community assessment. DNA提取方法在肠道真菌群落评价中的作用。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-02-06 DOI: 10.1016/j.ram.2024.12.005

Néstor D Portela, Cristian Mena, Mauricio G Martín, Verónica L Burstein, Laura S Chiapello, Susana A Pesoa

{"title":"Effect of DNA extraction method in gut fungal community assessment.","authors":"Néstor D Portela, Cristian Mena, Mauricio G Martín, Verónica L Burstein, Laura S Chiapello, Susana A Pesoa","doi":"10.1016/j.ram.2024.12.005","DOIUrl":"https://doi.org/10.1016/j.ram.2024.12.005","url":null,"abstract":"Understanding the gut mycobiota composition and its impact on health requires reliable methods for fungal community assessment. This study explores the influence of DNA extraction methods in GM analysis. Three protocols were evaluated: Qiagen DNeasy blood and tissue kit with mechanical glass bead lysis (DNgb), Thermofisher MagMax Microbiome ultra-nucleic isolation kit automated method (MM), and MM combined with glass beads lysis (MMgb). Fecal samples from healthy volunteers were collected, DNA extracted and ITS2 amplicon library preparation and sequencing performed. Results showed that DNA yields did not significantly differ among methods and the addition of glass bead beating favored the recovery of DNA more appropriate for fungal analysis. Beta diversity revealed distinct clusters, with MMgb showing the most pronounced variation in mycobiota composition, exposing particularly the low abundance taxa. LEfSe analysis identified significant differences in the abundance of fungal species among the extraction methods. Samples extracted with bead beating were enriched in filamentous species, while those without this step showed higher relative abundance of yeast fungi. This study underscores the importance of selecting appropriate DNA extraction methods for accurate characterization of the gut mycobiota, emphasizing the need for standardized methodologies to ensure reproducibility and reliability in microbial data acquisition.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Report of a field control experience of bovine viral diarrhea virus in 2 dairy farms of Argentina applying management, diagnostic, and vaccination tools]. [阿根廷2个奶牛场应用管理、诊断和疫苗接种工具对牛病毒性腹泻病毒的现场控制经验报告]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-01-27 DOI: 10.1016/j.ram.2024.12.001

Rodrigo Pereyra, Fernando Martino, Mariángeles Castillo, Juan Manuel Sala, Lucas José Barone, Claudio Paolazzi, Alejandra Victoria Capozzo

{"title":"[Report of a field control experience of bovine viral diarrhea virus in 2 dairy farms of Argentina applying management, diagnostic, and vaccination tools].","authors":"Rodrigo Pereyra, Fernando Martino, Mariángeles Castillo, Juan Manuel Sala, Lucas José Barone, Claudio Paolazzi, Alejandra Victoria Capozzo","doi":"10.1016/j.ram.2024.12.001","DOIUrl":"https://doi.org/10.1016/j.ram.2024.12.001","url":null,"abstract":"Bovine viral diarrhea virus (BVDV) causes significant economic losses in the international livestock industry and in Argentina, where it circulates at high prevalence. Under high prevalence conditions, BVDV infections are controlled through vaccination once persistently infected animals are identified and segregated. This study evaluated the feasibility of controlling BVDV circulation under field conditions by combining diagnosis, management measures, and vaccination in 2 dairy farms in the province of Santa Fe. Commercial ELISAs were used for the detection of the NS3 (P80) protein or antibodies against this protein as well as an RT-nested PCR for the detection of the viral genome, and viral seroneutralization to assess vaccine efficacy. The average seroprevalence of the farms was 58.4%, with a persistently infected animal rate of 2.4%. After segregating the persistently infected animals and vaccinating them with a commercial combined vaccine containing inactivated BVDV, abortion rates significantly decreased (p<0.05) in farm 1 (from 20.5 to 11.6%) and in farm 2 (from 34 to 23.4%) during the second year of the control strategy. Conception rates increased from 29 to 33% in farm 1 during the first year, while in farm 2, the increase was 7 points during the second year. This methodology achieved conditions in which BVDV ceased to circulate, constituting the first controlled report on BVDV management in dairy farms using tools available to producers in Argentina.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized DNA extraction protocol for Staphylococcus aureus strains utilizing liquid nitrogen. 利用液氮优化金黄色葡萄球菌DNA提取工艺。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-01-27 DOI: 10.1016/j.ram.2024.12.006

Mariana B Galeano, Stefania A Robaldi, Tania B Gordillo, Martiniano M Ricardi, Pablo M Cassanelli, Rosana O Pereda, Maria Mercedes Palomino, Paula Maria Tribelli

引用次数: 0