Revista Argentina de microbiologia最新文献

{"title":"Microbial diversity investigation using 16S metagenomics in Tunisian patients with systemic lupus erythematosus","authors":"Amira Messadi ,&nbsp;Sameh Sayhi ,&nbsp;Kais Ghedira ,&nbsp;Chadia Zaouaoui ,&nbsp;Bilel Arfaoui ,&nbsp;Soumaya Khouikhi ,&nbsp;Maha Rebai ,&nbsp;Nour El Houda Guediche ,&nbsp;Nadia Abdelhafidh ,&nbsp;Bassem Louzir ,&nbsp;Ajili Faida","doi":"10.1016/j.ram.2025.04.002","DOIUrl":"10.1016/j.ram.2025.04.002","url":null,"abstract":"<div><div>Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease associated with significant morbidity and mortality. It is characterized by a loss of self-immune tolerance and autoantibody production, leading to multiple organ damage. Emerging investigations have confirmed the role of gut microbiota dysbiosis in patients with SLE, although the underlying mechanisms remain unclear to date. In this study, we aim to investigate the bacterial profile of SLE including phylum/class/genus relative abundance and diversity, to compare them with healthy controls and to study the correlation of relative abundance of different patterns with clinical/biological parameters. In this case–control study, the bacterial profile was investigated in 7 SLE patients and 7 healthy controls using 16S metagenomics clustering. The present study reported a low abundance of the class Bacilli (0.58% in SLE vs 1.26% in the controls), the genus <em>Lactobacillus</em> (0.43% vs 0.74%), as well as a higher abundance of the genera <em>Gammaproteobacteria</em> (2.37% vs 0.77%) and <em>Escherichia</em>–<em>Shigella</em> (2.04% vs 0.51%) in SLE samples compared to the controls (<em>p</em> <!-->&lt;<!--> <!-->0.05). We also found an association between the class Betaproteobacteria (4.42% vs 1.57%) and the genus <em>Faecalibacterium</em> (11.34% vs 3.35%) and renal manifestations (<em>p</em> <!-->&lt;<!--> <!-->0.05). The phylum Actinobacteria (0.21% vs 3.8%, <em>p</em> <!-->=<!--> <!-->0.036) and the genus <em>Bifidobacterium</em> levels were lower in active SLE compared to the healthy controls. This study is the first report on the gut microbiota of SLE and the first case–control study in Tunisia and North Africa. We obtained a particular profile of bacterial gut microbiota for the SLE group. We found a specific clustering when compared to the healthy controls.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 275-287"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Análisis de muestras de kéfir de agua para el desarrollo de un marco regulatorio en el Código Alimentario Argentino","authors":"Manuel Teijeiro ,&nbsp;Analía Graciela Abraham ,&nbsp;Josefina Cabrera ,&nbsp;Galia Ana Suchowlanski ,&nbsp;Ana Losada ,&nbsp;Silvana Ruarte ,&nbsp;Mónica López ,&nbsp;Gabriel Vinderola","doi":"10.1016/j.ram.2025.02.002","DOIUrl":"10.1016/j.ram.2025.02.002","url":null,"abstract":"<div><div>Water kefir is a fermented beverage that has experienced a growing popular interest in its production, commercialization and consumption. However, it was not contemplated as such in the Argentine Food Code (CAA, Spanish acronym). The aim of this work was to determine some microbiological and physicochemical characteristics of water kefir samples produced by Argentine entrepreneurs, to have the necessary information for the National Food Commission (CONAL, Spanish acronym) to generate an article incorporating water kefir in the CAA. For this purpose, 31 water kefir producers from different cities of the country were invited to send samples of their products for microbiological and physicochemical analysis to the National Reference Laboratory of the National Food Institute (INAL, Spanish acronym) (Buenos Aires, Argentina). In the period December 2021-February 2022, a total of 13 samples were received, together with the corresponding information on their production process. The samples analyzed showed a pH between 2.9 and 3.7, a titratable acidity between 6 and 16.6 (ml of NaOH 1N/100<!--> <!-->ml of sample), an alcohol content between 0.58 and 2.55 (%v/v), a lactic acid bacteria count of 1<!--> <!-->×<!--> <!-->10⁷ <!-->CFU/ml and a yeast count of 1<!--> <!-->×<!--> <!-->10⁶ <!-->CFU/ml, with enterobacteria counts lower than 10<!--> <!-->CFU/ml). The results provided local data that culminated in the publication, on August 12, 2024, of the joint resolution 7/2024 of the Secretariat of Quality in Health and Secretariat of Bioeconomy by which article 1084 tris was incorporated to the CAA in the chapter XIII of fermented beverages, referring to water kefir.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 298-303"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospective evaluation of Leptospira spp. infection in goats maintained in field conditions in the Caatinga biome, Brazil","authors":"Mayla de Lisbôa Padilha,&nbsp;Lídio Ricardo Bezerra de Melo,&nbsp;Clécio Henrique Limeira,&nbsp;Nathália Maria de Andrade Magalhães,&nbsp;Maria Luana Cristiny Rodrigues Silva,&nbsp;Rafael Rodrigues Soares,&nbsp;Clebert José Alves,&nbsp;Severino Silvano dos Santos Higino,&nbsp;Carolina de Sousa Américo Batista Santos,&nbsp;Sérgio Santos de Azevedo","doi":"10.1016/j.ram.2025.03.001","DOIUrl":"10.1016/j.ram.2025.03.001","url":null,"abstract":"<div><div>The Caatinga biome occurs only in Brazil; however, there is no survey on leptospirosis using direct and indirect diagnostic tests in this region with goats maintained in field conditions. We conducted a prospective survey with paired sampling to evaluate the importance of carrier goats in the maintenance of disease. Based on sampling calculation, 60 goats (30 confined and 30 extensively reared) were randomly selected and monitored for three months during the rainy season with paired monthly biological sample collections. The animals underwent urine and vaginal fluid collection for microbiological and molecular diagnosis, and serum samples for serological diagnosis using the microscopic agglutination test (MAT). Overall, 45 (75%) animals were positive in at least one diagnostic method. Anti-<em>Leptospira</em> spp. antibodies were detected in all three sampling moments in 43 (71.7%) animals, antibody titers varied from 25 to 800, and most frequent serogroups were <em>Australis</em> in the 1st and 2nd blood collections (66.7% and 40.7%, respectively) and <em>Cynopteri</em> in the 3rd collection (52.6%). None of the animals tested positive in the microbiological diagnosis or the vaginal fluid PCR; however, five (16.7%) animals were positive in the urine PCR only in the confined group in the first collection. Two DNA urine samples were sequenced, demonstrating 99% similarity with <em>Leptospira interrogans</em>. Different diagnostic techniques for leptospirosis in goats raised under Caatinga biome field conditions are suggested, as well as further studies over a longer period with monthly collections to better understand the prevalence of <em>Leptospira</em> spp. and its variation over time.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 265-274"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an enzyme-linked immunosorbent assay for detection of antibodies against infectious bursal disease virus based on a version of the recombinant viral protein 2","authors":"Leticia Keller,&nbsp;Carina Romanutti,&nbsp;Flavia Adriana Zanetti","doi":"10.1016/j.ram.2024.09.003","DOIUrl":"10.1016/j.ram.2024.09.003","url":null,"abstract":"<div><div>Infectious bursal disease virus (IBDV) is the etiological agent of a highly contagious and immunosuppressive disease in chickens. In poultry farms, the level of anti-IBDV antibodies of numerous serum samples must be monitored using fast and simple methodologies. Therefore, the aim of this study was to develop an enzyme-linked immunosorbent assay (ELISA), in an indirect format, using a version of mature viral protein 2 (VP2) of IBDV as coating agent. This recombinant fusion protein (His-VP2) was expressed at high levels in <em>Escherichia coli</em>. Bacterial inclusion bodies containing His-VP2 were successfully recovered using a simple, inexpensive and efficient method, a further purification of recombinant protein by affinity chromatography using immobilized metal chelates being unnecessary. After the VP2-ELISA was optimized, its performance was evaluated using preanalyzed sera from uninfected specific pathogen-free chickens and broilers vaccinated against IBDV in poultry farms, using a commercial ELISA kit. Based on these results, the developed assay proved to be sensitive, specific and in high agreement with the kit available on the market. In addition, the in-house ELISA demonstrated to be reproducible by intra-assay and inter-assay variability studies. In conclusion, VP2-ELISA could be an efficient and low-cost alternative diagnostic method to detect antibodies to IBDV in the poultry industry.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 208-216"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized DNA extraction protocol for Staphylococcus aureus strains utilizing liquid nitrogen","authors":"Mariana B. Galeano ,&nbsp;Stefania A. Robaldi ,&nbsp;Tania B. Gordillo ,&nbsp;Martiniano M. Ricardi ,&nbsp;Pablo M. Cassanelli ,&nbsp;Rosana O. Pereda ,&nbsp;Maria Mercedes Palomino ,&nbsp;Paula Maria Tribelli","doi":"10.1016/j.ram.2024.12.006","DOIUrl":"10.1016/j.ram.2024.12.006","url":null,"abstract":"<div><div>DNA extraction is crucial for conducting procedures, such as whole-genome sequencing, which demand methods that are reproducible and cost-effective. Lysing <em>Staphylococcus aureus</em> cells is particularly challenging due to their peptidoglycan layer that is resistant to common treatments. Traditional methods involve costly enzymatic lysis using lysostaphin. Here, we developed a novel approach for lysis utilizing liquid nitrogen and mechanical disruption in a mortar. DNA from <em>S. aureus</em> USA300 and related clinical isolates were purified using phenol–chloroform extraction followed by precipitation. The integrity and purity of DNA were confirmed, obtaining suitable concentration and purity for various molecular biology techniques. The quality of the employed DNA was validated by amplifying fragments of different genes using PCR. This method circumvents lysostaphin, yielding DNA that is suitable for use in other techniques.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 217-220"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition in phyla from breast milk: Effect of the mode of delivery","authors":"Shiva Aslani ,&nbsp;Seyedeh Neda Mousavi ,&nbsp;Atefeh Kazemi Robati ,&nbsp;Siamak Heidarzadeh ,&nbsp;Somaye Abdollahi Sabet","doi":"10.1016/j.ram.2025.04.001","DOIUrl":"10.1016/j.ram.2025.04.001","url":null,"abstract":"<div><div>Breast milk is considered as a living ecosystem. Maternal, environmental and neonatal factors affect milk bacterial composition. The aim of present study was to assess the phyla from breast milk of mothers with vaginal delivery compared to the cesarean section. In this single-center case–control study, sixty women were participated. Half of them had vaginal delivery and others experienced cesarean section. The breast milk samples were collected three months after delivery for the DNA extraction to measure Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria using quantitative real time chain polymerase reaction. Daily intake of calories, protein, fat, carbohydrate and fiber did not differ significantly between the two groups. The proportion of Bacteroidetes, Firmicutes and Proteobacteria were significantly higher in milk of mothers with a cesarean section than the vaginal delivery (<em>p</em> <!-->=<!--> <!-->0.03, <em>p</em> <!-->=<!--> <!-->0.02 and <em>p</em> <!-->=<!--> <!-->0.042). Similarly, the Firmicutes to Bacteroides ratio was significantly increased (<em>p</em> <!-->=<!--> <!-->0.02). The Actinobacteria population was significantly higher in milk of vaginally-delivered mothers who had male infant than females (<em>p</em> <!-->=<!--> <!-->0.015). Breast milk of mothers with cesarean section showed alterations in the main bacterial phyla population compared to the vaginal delivery. Moreover, our results suggest that the sex of infant is an effective factor on some phyla quantity.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 231-238"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriemia por Salmonella enterica no Typhi atípica, lactosa positiva, lisina decarboxilasa negativa","authors":"Susana D. García ,&nbsp;María I. Caffer ,&nbsp;Marisa N. Almuzara ,&nbsp;Ángela M.R. Famiglietti ,&nbsp;Cristina Myburg ,&nbsp;María R. Viñas ,&nbsp;Jorge Risso ,&nbsp;Carlos A. Vay","doi":"10.1016/j.ram.2024.09.007","DOIUrl":"10.1016/j.ram.2024.09.007","url":null,"abstract":"<div><div>We describe here the first isolation in Argentina of a lactose-fermenting and lysine decarboxylase-negative non-Typhi <em>Salmonella enterica</em> from a blood culture of a patient with systemic lupus erythematosus. The microorganism was identified as <em>S. enterica</em> (score: 2.148) by mass spectrometry, as “<em>Salmonella</em> group” (discrimination: 86%) by the VITEK® 2 system and as <em>Citrobacter freundii complex</em> by biochemical tests. The serological test using the OMA antiserum for the somatic antigen was positive. The identification was confirmed as <em>Salmonella</em> subsp. I 4,[5],12:i:- atypical at the National Reference Center ANLIS Dr. Carlos G. Malbrán. Mass spectrometry is especially useful in microbial identification in the presence of atypia, while biochemical tests can lead to misidentification in these cases. This study warns about the circulation of this strain in Argentina.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 251-255"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro activity of ceftobiprole against Staphylococcus aureus: Current experience","authors":"Manuel Paz-Infanzon ,&nbsp;Adrian Camacho-Ortiz ,&nbsp;Eduardo Perez-Alba ,&nbsp;Laura Nuzzolo-Shihadeh ,&nbsp;Rayo Morfin-Otero ,&nbsp;Paola Bocanegra-Ibarias","doi":"10.1016/j.ram.2024.12.011","DOIUrl":"10.1016/j.ram.2024.12.011","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 239-240"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Escherichia coli isolated from minced meat: Implications for public health in Tierra del Fuego, Argentina.","authors":"Elisa Crespi, Bárbara Ghiglione, Ximena Blanco Crivelli, Cecilia Cundon, Roque Figueroa Espinosa, Pedro Penzotti, Ezequiel Nuske, Alicia Broglio, Luis Ambros, María Valeria Rumi, Gabriela Albarellos, José Di Conza, Adriana Bentancor","doi":"10.1016/j.ram.2025.05.001","DOIUrl":"10.1016/j.ram.2025.05.001","url":null,"abstract":"<p><p>Escherichia coli contamination in minced meat poses a significant public health concern and may serve as a reservoir for antimicrobial-resistant strains. This study investigated the presence of E. coli resistant to ciprofloxacin and/or gentamicin in minced meat samples from Tierra del Fuego, Argentina. Resistant E. coli isolates were identified using MALDI-TOF. A total of 109 samples were analyzed, yielding 97 E. coli isolates (23 from AMC-CIP and 74 from AMC-GEN). Twenty-three isolates carried the ant(2″) gene, ten the ant(3″) gene, and one the blaCTX-M gene. Resistance was observed to gentamicin (6/25), streptomycin (1/25), ceftazidime (1/25), and cefotaxime (4/25). Eleven isolates carried the aggR gene, and another three were identified as biofilm producers. Although phenotypic resistance was low, we detected genetic potential for resistance which, combined with biofilm formation, represents a risk to food safety.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors associated with neonatal sepsis due to Serratia marcescens in a public hospital in Mexico: A case-control study.","authors":"Juan Carlos Lona-Reyes, Lucia Miramontes-Buiza, Luis Fernando Frías-Flores, Jose Angel Regla-Nava, Jesús González-Carmona, Juan Antonio Gallegos-Marín, Mariana Chávez-Rodríguez","doi":"10.1016/j.ram.2025.04.006","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.006","url":null,"abstract":"<p><p>This study aimed to identify factors associated with neonatal sepsis due to Serratia marcescens in a Neonatal Intensive Care Unit (NICU) in Mexico. A case-control study was conducted, matched by gestational age and birth weight. The analysis was performed using logistic regression. Seventy newborns with S. marcescens sepsis were identified; the median gestational age was 34.4 weeks, and the median birth weight was 1680g. Microbial isolates were identified in the bloodstream in 98.6% of cases, and 88.6% of these were diagnosed after 72h of life. Independent variables associated with infection were the presence of a central venous catheter (OR 5.17; 95% CI 1.57-17.1), mechanical ventilation (OR 3.23; 95% CI 1.18-8.87), and prior use of meropenem (OR 3.38; 95% CI 1.01-11.3). The case fatality rate of the infection was 32.9%.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}