Revista Argentina de microbiologia最新文献

{"title":"Health-promoting peptides in fermented beverages","authors":"","doi":"10.1016/j.ram.2024.02.003","DOIUrl":"10.1016/j.ram.2024.02.003","url":null,"abstract":"<div><p>Since ancient times, the consumption of fermented low-alcoholic beverages has enjoyed widespread popularity in various countries, because of their distinct flavors and health benefits. Several studies have demonstrated that light to moderate alcohol consumption is associated with beneficial effects on human health, mainly in cardiovascular disease prevention. Fermented beverages have different non-ethanol components that confer beneficial health effects. These bioactive compounds are mainly peptides that have often been overlooked or poorly explored in numerous fermented beverages. The aim of this review is to provide knowledge and generate interest in the biological activities of peptides that are present and/or released during the fermentation process of widely consumed traditional fermented beverages. Additionally, a brief description of the microorganisms involved in these beverages is provided. Furthermore, this review also explores topics related to the detection, isolation, and identification of peptides, addressing the structure–activity relationships of both antioxidant and angiotensin-converting enzyme inhibitory (ACE-I) activities.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000221/pdfft?md5=082941b8b422c8e0bc4609d3af1fda8d&pid=1-s2.0-S0325754124000221-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140790968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primer aislamiento de Acanthamoeba spp. en agua de mar del sudeste bonaerense, Argentina","authors":"","doi":"10.1016/j.ram.2024.02.006","DOIUrl":"10.1016/j.ram.2024.02.006","url":null,"abstract":"<div><p>Free-living amoebae (FLA) of the genus <em>Acanthamoeba</em> are ubiquitous and amphizoic protozoa that colonize aquatic and terrestrial habitats and can serve as reservoirs for other microorganisms. They are considered econoses that can cause severe and rare pathologies. Due to limited epidemiological data available, the objective of this study was to investigate the presence of <em>Acanthamoeba</em> in coastal wetlands of the southeast of Buenos Aires province and evaluate their association with bacteriological and environmental variables. From February 2021 to July 2022, 22 seawater samples were collected at different points along the coast of the city of Mar del Plata (Buenos Aires, Argentina). Environmental parameters were determined and physicochemical and bacteriological studies, morphological identification, cultures and molecular typification were conducted. Regardless of the environmental and bacteriological variables, the presence of <em>Acanthamoeba</em> spp. was molecularly confirmed in 54.54% of the samples, being the first report of these protozoa in seawater in Argentina.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000385/pdfft?md5=8a7ab49ad87cd955e7725eb083d57278&pid=1-s2.0-S0325754124000385-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140791714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of monkeypox virus in central Argentina: Epidemiological features and first complete genome sequences in the country","authors":"Gonzalo M. Castro ,&nbsp;Paola E. Sicilia ,&nbsp;Ana Willington ,&nbsp;Laura López ,&nbsp;Tomás Poklepovich ,&nbsp;Josefina Campos ,&nbsp;María G. Barbás","doi":"10.1016/j.ram.2024.05.003","DOIUrl":"10.1016/j.ram.2024.05.003","url":null,"abstract":"<div><p>Monkeypox (Mpox) is a zoonotic disease caused by the monkeypox virus (MPXV). MPXV can be transmitted by close contact with lesions, body fluids, respiratory droplets, and contaminated materials. A new pattern of spread among sexual networks has been recently described. The present work aimed to report the epidemiological and genomic characterization of the 2022 MPXV outbreak in central Argentina. A total of 113 scabs and/or lesion swab specimens were studied. MPXV infection was confirmed in 46.0% of the studied patients, all of whom were men. Varicella-zoster virus infection was the most frequent differential diagnosis. Eight complete viral genomes were obtained by next-generation sequencing. The Argentinian sequences were grouped intermingled with other sequences from the 2022 MPXV outbreak, related to samples from the USA, Europe, and Peru. Taken together, our study provided an initial assessment of the genetic and epidemiological characteristics of the 2022 MPXV outbreak in Córdoba, Argentina.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000774/pdfft?md5=19901670a2a3199969206463fb17a33b&pid=1-s2.0-S0325754124000774-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a mixed additive based on Saccharomyces cerevisiae and Lactobacillus rhamnosus on broilers exposed to aflatoxin B1 by contaminated feed","authors":"Analía Silvia Fochesato ,&nbsp;María Pia Martínez ,&nbsp;Débora Cuello ,&nbsp;Valeria Lorena Poloni ,&nbsp;María Julieta Luna ,&nbsp;Alejandra Paola Magnoli ,&nbsp;Cristian Fernández ,&nbsp;Lilia Renée Cavaglieri","doi":"10.1016/j.ram.2023.11.006","DOIUrl":"10.1016/j.ram.2023.11.006","url":null,"abstract":"<div><p>The objective of the present study was to explore the influence of dietary supplementation with a mixed additive (MA) containing a probiotic and anti-mycotoxin (<em>Saccharomyces cerevisiae</em> RC016 and <em>Lactobacillus rhamnosus</em> RC007) and its interaction on the performance and health (biochemistry and liver/intestine histopathology) of broilers fed diets contaminated with aflatoxin B₁ (AFB₁) at 506<!--> <!-->000<!--> <!-->±<!--> <!-->22.1<!--> <!-->ng/kg. The MA contained <em>S. cerevisiae</em> RC016 (1<!--> <!-->×<!--> <!-->10⁷ <!-->cells/g) and <em>L. rhamnosus</em> RC007 (1<!--> <!-->×<!--> <!-->10⁸ <!-->cells/g) in relation 1:1. A total of sixty-one-day-old Cobb broilers were randomly allocated into four treatment groups with three replicates of 5 birds each for a five-week-old feeding experiment. The experimental diet for each treatment (T) was formulated as follows: T1, a commercial diet (CD); T2, CD<!--> <!-->+<!--> <!-->AFB₁; T3, CD<!--> <!-->+<!--> <!-->0.1% MA; T4, CD<!--> <!-->+<!--> <!-->AFB₁ <!-->+<!--> <!-->0.1% MA. The MA improved (<em>p</em> <!-->&lt;<!--> <!-->0.01) production parameters (weight gain, conversion rate, and carcass yield) and reduced (<em>p</em> <!-->&lt;<!--> <!-->0.01) the toxic effect of AFB₁ on the relative weight of the livers. In addition, the macro and microscopic alterations of livers and the possible intestinal injury related to histological damage in the presence of mycotoxin were reduced. The use of probiotic MA based on <em>S. cerevisiae</em> RC016 and <em>L. rhamnosus</em> RC007 in animal feed provides greater protection against mycotoxin contamination and is safe for use as a supplement in animal feed, providing beneficial effects that improve animal health and productivity. This is of great importance at the economic level for the avian production system.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000804/pdfft?md5=68ff0cace867ee2cdecf71f3b49554e1&pid=1-s2.0-S0325754124000804-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of vaccination with Brucella melitensis, strains Rev 1 ΔeryCD and Rev 1, on the reproductive system of young male goats","authors":"","doi":"10.1016/j.ram.2023.11.005","DOIUrl":"10.1016/j.ram.2023.11.005","url":null,"abstract":"<div><p>The present study evaluates the effects of vaccination with <em>Brucella melitensis</em> strains Rev 1 <em>ΔeryCD</em> and Rev 1 on the reproductive system of male goats. Three groups, each of them consisting of 15 six-month-old brucellosis-free male goats, were studied. The first group was vaccinated with the Rev 1 <em>ΔeryCD</em> strain, the second group received Rev 1 and the third group was inoculated with sterile physiological saline solution. The dose of both strains was of 1<!--> <!-->×<!--> <!-->10⁹ <!-->CFU/ml. Over the course of the five months of this study, three males from each group were euthanized every month. Their reproductive tracts, spleens, and lymph nodes were collected to analyze serology, bacteriology PCR, histology, and immunohistochemistry. Results show that vaccination with <em>B. melitensis</em> strains Rev 1 ΔeryCD and Rev 1 does not harm the reproductive system of male goats. Strain <em>B. melitensis</em> Rev 1 <em>ΔeryCD</em> displayed a lower capacity to colonize the reproductive tract than strain Rev 1, which was attributed to its limited catabolic action toward erythritol.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000051/pdfft?md5=adfb14f9a36ad3c95ab9bd88af08083b&pid=1-s2.0-S0325754124000051-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140055899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identificación y actividad antifúngica de bacterias halófilas aisladas de suelos salinos en Campeche, México","authors":"","doi":"10.1016/j.ram.2024.02.004","DOIUrl":"10.1016/j.ram.2024.02.004","url":null,"abstract":"<div><p>Phytopathogenic fungi <em>Alternaria alternata</em> and <em>Colletotrichum gloeosporioide</em>s cause diseases in plant tissues as well as significant postharvest losses. The use of chemical fungicides for their control has negative effects on health and the environment. Secondary metabolites from halophilic bacteria are a promising alternative for new antifungal compounds. In the present study, halophilic bacteria were isolated and characterized from two sites with saline soils called branquizales in Campeche, Mexico. A total of 64 bacteria were isolated. <em>Agrobacterium</em>, <em>Bacillus</em>, <em>Inquilinus</em>, <em>Gracilibacillus</em>, <em>Metabacillus</em>, <em>Neobacillus</em>, <em>Paenibacillus</em>, <em>Priestia</em>, <em>Staphylococcus</em>, <em>Streptomyces</em> and <em>Virgibacillus</em> were among the identified genera. The antifungal potential of the culture supernatant (CS) of 39 halophilic bacteria was investigated against <em>C</em>. <em>gloeosporioides</em> and <em>A</em>. <em>alternata</em>. The bacteria showing the greatest inhibition of mycelial growth corresponded to <em>Bacillus subtilis</em> CPO 4292, <em>Metabacillus</em> sp. CPO 4266, <em>Bacillus</em> sp. CPO 4295 and <em>Bacillus</em> sp. CPO 4279. The CS of <em>Bacillus</em> sp. CPO 4279 exhibited the highest activity and its ethyl acetate extract (AcOEt) inhibited the germination of <em>C</em>. <em>gloeosporioides</em>, with IC₅₀ values of 8,630<!--> <!-->μg/ml and IC₉₀ of 10,720<!--> <!-->μg/ml. The organic partition of the AcOEt extract led to three fractions, with acetonitrile (FAcB9) showing the highest antifungal activity, with values exceeding 66%. Halophilic bacteria from ‘blanquizales’ soils of the genus <em>Bacillus</em> sp. produce metabolites with antifungal properties that inhibit the phytopathogenic fungus <em>C</em>. <em>gloeosporioides</em>.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000233/pdfft?md5=9262279ae0a4f9cff2fa928452c96f9c&pid=1-s2.0-S0325754124000233-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140788291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabiotics: do we need a new definition?","authors":"Gabriel Vinderola ,&nbsp;Marcela Carina Audisio","doi":"10.1016/j.ram.2024.09.002","DOIUrl":"10.1016/j.ram.2024.09.002","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124001081/pdfft?md5=6a11b3b1aa76572fb8cf890bcd0d81b2&pid=1-s2.0-S0325754124001081-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142238802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bartonella spp. in different species of bats from Misiones (Argentina)","authors":"","doi":"10.1016/j.ram.2024.04.001","DOIUrl":"10.1016/j.ram.2024.04.001","url":null,"abstract":"<div><p>The aim of this study was to detect vector-borne pathogens (Anaplasmataceae family, <em>Rickettsia</em> genus, and <em>Bartonella</em> genus) in bats from Misiones (Argentina). Thirty-three specimens were captured over 8 days using mist nets. Twenty (60.6%) blood samples were positive (11/13 <em>Artibeus lituratus</em>, 4/10 <em>Desmodus rotundus</em>, 4/8 <em>Carollia perspicillata</em>, and 1/2 <em>Myotis nigricans</em>) by PCR for the <em>gltA</em> gene fragment of <em>Bartonella</em>. All samples were negative by PCR for the <em>Anaplasmataceae</em> family and <em>Rickettsia</em> genus.</p><p>The phylogenetic analysis showed seven <em>Bartonella</em> genotypes. The three genotypes obtained from <em>A. lituratus</em>, 2 from <em>C. perspicillata</em>, and 1 from <em>D. rotundus</em> were related to <em>Bartonella</em> spp. from New World bats, while the sequence obtained from <em>M. nigricans</em> was related to Old World bats.</p><p>We identified a considerable diversity of <em>Bartonella</em> genotypes in a small number of bats, thus further research is required to better understand the complex bat–pathogen interaction.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000452/pdfft?md5=d4d6bdb92e2b6e7dc7c4ee7c253ddfed&pid=1-s2.0-S0325754124000452-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141318178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsporum canis pseudomycetoma and disseminated Mycobacterium genavense infection in an HIV/AIDS patient, an unusual combination","authors":"","doi":"10.1016/j.ram.2024.02.005","DOIUrl":"10.1016/j.ram.2024.02.005","url":null,"abstract":"<div><p>Pseudomycetomas are rare fungal subcutaneous infections caused by dermatophytes, which are mainly observed in immunocompromised patients. <em>Mycobacterium genavense</em> is considered an opportunistic pathogen in people living with HIV/AIDS (PLWHA), clinically resembling the presentation of <em>Mycobacterium avium</em> complex (MAC). Here, we describe the case of a 26-year-old PLWHA with a 3-month history of a 4<!--> <!-->cm tumoral, duroelastic and painful lesion located on the back. Histopathology of the tumoral lesion revealed chronic granulomatous inflammation with grains composed of PAS-positive and Grocott-positive septate hyphae, as well as acid-fast bacilli (AFB). Culture on Sabouraud and lactrimel agar developed colonies that were later identified as <em>Microsporum canis</em>. In successive samples, the AFB were identified as <em>M. genavense</em> by restriction analysis of PCR products. Immunocompromised PLWHA not only suffer increased susceptibility to diseases due to unusual pathogens but also atypical clinical presentation of frequently encountered pathogens.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000373/pdfft?md5=3e3d27183b4c9a9e346534784754f925&pid=1-s2.0-S0325754124000373-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140781874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detección de microorganismos y genes de resistencia en hemocultivos positivos mediante FilmArray® panel BCID2: experiencia en un hospital pediátrico","authors":"Marilina Kuzawka,&nbsp;Martín Cassanelli,&nbsp;Fabrina Capecce,&nbsp;Rosana Pereda","doi":"10.1016/j.ram.2023.07.007","DOIUrl":"10.1016/j.ram.2023.07.007","url":null,"abstract":"<div><p>The rapid identification of microorganisms that cause bacteremia and their possible resistance markers are extremely important for the timely initiation of effective antibiotic therapy.</p><p>The FilmArray® panel BCID2 (an automated rapid <em>multiplex</em> PCR assay) detects microorganisms and resistance genes from positive blood cultures within one hour. The aim of this study was to compare the results obtained from the FilmArray® Panel BCID2 and conventional culture in pediatric patients, as well as the reporting times of both methods. Sixty (60) FilmArray® results were included in the analysis. BCID2 showed high agreement with culture in the identification of microorganisms in monomicrobial bacteremias. However, in polymicrobial blood cultures, BCID2 detected a greater number of microorganisms compared to conventional culture, specifically,1 <em>Staphylococcus aureus</em>, 3 <em>Staphylococcus epidermidis</em>, 1 <em>Enterococcus faecium</em>, 2 <em>Klebsiella oxytoc</em>a, 1 <em>Acinetobacter calcoaceticus-baumannii</em> complex, 1 <em>Bacteroides fragilis</em> and 1 <em>Haemophilus influenzae</em>. Furthermore, 88.3% (95%<span>C</span>I<span>:</span> 78.7-94.8) of the FilmArray® results coincided with conventional culture, while in polymicrobial bacteremias, BCID2 detected a greater number of microorganisms with respect to conventional culture [70% (95%CI: 39.3-91.5)]. The agreement of resistance genes was good with a few exceptions (one ESBL was not detected by FilmArray® and one <em>S. aureus</em> strain was characterized as methicillin-resistant by BCID2 and methicillin-sensitive by culture). When comparing the time elapsing since the blood culture was reported as positive up to the results were obtained, BCID2 had a median of 2<!--> <!-->h 50<!--> <!-->min (IQR of 1 h 58<!--> <!-->min to 9<!--> <!-->h 27<!--> <!-->min) while the conventional culture had a median of 45<!--> <!-->h 20<!--> <!-->min (IQR of 24<!--> <!-->h 57<!--> <!-->min to 63<!--> <!-->h 50<!--> <!-->min).</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000786/pdfft?md5=27adedd3b1f845cddbdc1d92d35b565f&pid=1-s2.0-S0325754124000786-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141856316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}