Revista Argentina de microbiologia最新文献

{"title":"Effect of probiotic bacteria, phycocyanin and tarragon essential oil on the physiochemical, microbiological, and sensory properties of a mouth freshener tablet","authors":"Fatemeh Habibi ,&nbsp;Bahareh Nowruzi ,&nbsp;Maryam Ghane","doi":"10.1016/j.ram.2025.04.004","DOIUrl":"10.1016/j.ram.2025.04.004","url":null,"abstract":"<div><div>Microbial pigments are extensively used in industry as food coloring, dyes, and medicinal substances. This study aimed to develop a probiotic mouth freshener tablet containing PC, tarragon essential oil, and microencapsulated <em>Lactobacillus plantarum</em> and investigate its antimicrobial properties against <em>Streptococcus mutans</em>. First, cyanobacterium <em>Spirulina</em> sp. was cultured and PC extracted. Then, microbial suspension and microencapsulation of probiotic bacteria, tarragon essential oil extraction, as well as mouth freshener tablets were prepared. Four treatments (T) under Co: control sample, T₁, T₂, T₃, and T₄ contained 1% PC, 1% microcoating probiotic, 0.8% tarragon essential oil, and 1% PC<!--> <!-->+<!--> <!-->1% microcoating probiotic<!--> <!-->+<!--> <!-->0.8% tarragon essential oil, were prepared respectively. The results of the inhibition zone diameter of samples also indicated that the highest and lowest sensitivity were observed in T₄ and T₁, respectively. Based on the results, the addition of PC and tarragon essential oil significantly increased the antioxidant activity of the samples (<em>p</em> <!-->&lt;<!--> <!-->0.05). The results indicated that the release rate of probiotic bacteria under gastric conditions in T₄ was lower compared to that in treatment T₂ (<em>p</em> <!-->&lt;<!--> <!-->0.05). Sensory evaluation results showed that the highest values were observed in T₃ and T₄ (<em>p</em> <!-->&lt;<!--> <!-->0.05).</div></div><div><h3>Conclusion</h3><div>Overall, it can be concluded that the prepared mouth freshener tablets (T₄) can be effective agents for oral disinfection and the promotion of public health.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"58 1","pages":"Pages 6-17"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and genomic surveillance of invasive Neisseria meningitidis isolates in Argentina (2015–2022): Clonal structure and vaccine implications","authors":"Adriana Efron ,&nbsp;Luciana Moreira ,&nbsp;Federico Lorenzo ,&nbsp;Denise De Belder ,&nbsp;María Moscoloni ,&nbsp;Alejandra Corso ,&nbsp;Mauricio Santos ,&nbsp;Tomás Poklepovich ,&nbsp;Josefina Campos ,&nbsp;Argentinean N. meningitidis Working Group ,&nbsp;Paula Gagetti ,&nbsp;María Sol Haim","doi":"10.1016/j.ram.2025.04.005","DOIUrl":"10.1016/j.ram.2025.04.005","url":null,"abstract":"<div><div>Invasive meningococcal disease is associated with high fatality rates and disabling sequelae. The aims of this study were to describe the phenotypic and genotypic characteristics of invasive <em>Neisseria meningitidis</em> isolates in Argentina, determine the genomic population structure and evaluate the potential coverage of vaccines targeting <em>N. meningitidis</em> serogroup B (MenB). During the period 2015–2022, a total of 444 isolates of <em>N. meningitidis</em> causing invasive disease were assessed through laboratory surveillance; 344 isolates were available for whole genome sequencing characterization. The presence of antimicrobial resistance genes, and the distribution of clonal complexes and vaccine antigens were analyzed. MenB was the most frequent serogroup (53.2%), followed by MenW (32.0%). Most isolates were susceptible to the antimicrobial agents tested; however, 56.7% exhibited intermediate resistance to penicillin. MenB showed wide genetic diversity and was mainly associated with ST-865 cc, ST-35 cc, ST-41/44 cc, and ST-32 cc. Unlike other countries, in Argentina, ST-865 cc was one of the major clonal complexes associated with MenB, MenW was only associated with ST-11 cc. To study the potential coverage of vaccines targeting MenB, we used the MenDeVAR (Meningococcal Deduced Vaccine Antigen Reactivity) index, which provides information on the presence and possible immunological cross-reactivity of the different vaccine antigenic variants. MenDeVar showed 19.3% vaccine reactivity for 4CMenB and 40.0% for the bivalent vaccine. Laboratory surveillance is essential for generating evidence-based decisions for updating vaccination strategies.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"58 1","pages":"Pages 40-56"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salmonella ser. Enteritidis, Salmonella ser. Typhimurium/4,[5],12:i:- and Salmonella ser. Typhi biomarkers detection using Biotyper MicroFlex LT and VITEK MS PRIME platforms in Argentina","authors":"Agustina Costa ,&nbsp;Andrea Alcain ,&nbsp;Marcela Panagopulo ,&nbsp;Jesús Emmanuel Riquel Moyelak ,&nbsp;Mirian Moroni ,&nbsp;Florencia Catalano ,&nbsp;Silvina Brengi ,&nbsp;María Florencia Rocca ,&nbsp;María Rosa Viñas","doi":"10.1016/j.ram.2025.09.007","DOIUrl":"10.1016/j.ram.2025.09.007","url":null,"abstract":"<div><div><em>Salmonella</em> genus includes around 2600 serovars, causing important zoonotic diseases and enteric fever, with significant impact on public health worldwide. Serovar characterization is commonly achieved by phenotypic and genotypic tests, requiring several days to obtain a result. Mass spectrometry (MS) only allows identification of <em>Salmonella</em> isolates at the genus level. Our aim was to evaluate the performance of MS for <em>Salmonella</em> ser. Enteritidis (SE), <em>Salmonella</em> ser. Typhimurium (STM)/4,[5],12:i:- and <em>Salmonella</em> ser. Typhi (ST) specific-serovar biomarker detection. We included 257 isolates from 34 different serovars (62 SE, 69 STM/4,[5],12:i:-, 17 ST) for analysis of spectra obtained by the direct method, using CHCA as matrix. For acquisition, we used Biotyper MicroFlex LT (Bruker Daltonics) and VITEK MS PRIME (BioMérieux) platforms, and performed a manual search for previously described biomarkers. Using Biotyper MicroFlex LT, sensitivity (Se) for the 3016<!--> <!-->Da and 6036<!--> <!-->Da SE biomarkers was 85% and 95%, respectively (specificity (Sp)<!--> <!-->=<!--> <!-->100%). The Se for the STM/4,[5],12:i:- biomarker (7095<!--> <!-->Da) was 100% and the Sp<!--> <!-->=<!--> <!-->98%. For ST biomarker (5710<!--> <!-->Da), Se and Sp were 88% and 100%, respectively. For VITEK MS PRIME, Se for the 3018<!--> <!-->Da SE biomarker was 42% and for the 6036<!--> <!-->Da was 79% (Sp<!--> <!-->=<!--> <!-->100% for both); for the STM/4,[5],12:i:- biomarker (7096<!--> <!-->Da), Se was 83% and Sp<!--> <!-->=<!--> <!-->97%; and for the ST biomarker (5712<!--> <!-->Da), Se<!--> <!-->=<!--> <!-->94% and Sp<!--> <!-->=<!--> <!-->100%. The identification of these frequent serovars can be expedited using MALDI-TOF MS technology. The rapid and reliable diagnosis of salmonellosis can have a positive impact on patient outcomes and public health.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"58 1","pages":"Pages 66-73"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145638144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caracterización molecular del gen ORF5 (GP5) del agente causal del síndrome respiratorio y reproductivo porcino (PRRS) detectado en granjas porcinas de Lima, Perú","authors":"Rony Cotaquispe Nalvarte ,&nbsp;Miriam Legua Barrios ,&nbsp;Patricia Escajadillo Luján ,&nbsp;Edgard De la Cruz Vásquez ,&nbsp;Jennifer Toskano Hurtado","doi":"10.1016/j.ram.2025.07.005","DOIUrl":"10.1016/j.ram.2025.07.005","url":null,"abstract":"<div><div>The porcine reproductive and respiratory syndrome (PRRS) is an endemic disease in pork-producing regions of the world, and its control remains poor. Rapid identification of PRRSV-1 and PRRSV-2 species is of great importance for molecular epidemiological surveillance of the virus. The objective of this study was the molecular characterization of the <em>ORF5</em> gene that synthesizes glycosylated protein 5 (GP5) from PRRS virus detected in pig farms in Lima, Perú. One hundred serum samples were used, obtaining 24 PRRSV-2-positive strains confirmed by two molecular techniques. Phylogenetic inference demonstrated the parental relationship with the NADC34 variant, and we achieved a better phylogenetic relationship of the study strains with Peruvian reference isolates in lineage 1 and sublineage 1.5 and pathogenic strains from various parts of the world. Homology analysis showed that strains 25 to 35, 40 to 41, and 43 to 48 obtained high nucleotide homology values of 95-98% and amino acid homology of 92-98%; strains 36, 37, 38, and 39 with high genetic variability with low nucleotide homology values of 83% and amino acid homology of 79%, and strain 42 with homology of 89 and 84% in relation to the NADC34 variant. In conclusion, we demonstrated the effectiveness of the validated in-house primers for identification compared to a commercial kit, in addition to the evolution and dispersion of lineage 1, sublineage 1.5 and the phylogenetic relationship with the NADC34 variant of the PRRSV-2 species in Lima, Perú.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"58 1","pages":"Pages 23-30"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tuberculosis after solid organ transplantation: Clinical experience in a tertiary hospital from Argentina","authors":"Patricia Olga Andres ,&nbsp;Elizabeth Madsen ,&nbsp;Analía Laura Fernández","doi":"10.1016/j.ram.2025.09.001","DOIUrl":"10.1016/j.ram.2025.09.001","url":null,"abstract":"<div><div>Tuberculosis in solid organ transplant (SOT) recipients is a clinical challenge. This opportunistic infection has atypical presentations and raises concerns due to both the toxicity of antifimic drugs and their interaction with immunosuppressive therapy that may result in graft loss or death. This retrospective review of cases of active tuberculosis after SOT describes the management of this infection in a hospital in Argentina. Between January 2006 and June 2022, 27 transplanted patients had positive cultures for the <em>Mycobacterium tuberculosis</em> complex. Their median age was 56 years; 78% were male. Ten (37%) patients had extra-pulmonary or disseminated tuberculosis. Twenty-five (93%) patients required invasive procedures to reach a diagnosis. In 17 (63%) patients, the initial diagnosis was based on a positive Ziehl-Neelsen smear. Twenty-four patients received a four-drug induction treatment without rifampin. Clinical cure was 80% and crude mortality was 20%.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"58 1","pages":"Pages 60-65"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estrategias para el diagnóstico y monitoreo de citomegalovirus en pacientes trasplantados","authors":"María Daniela Borgnia ,&nbsp;María Vanesa Romano ,&nbsp;Marilina Rahhal ,&nbsp;Ana María Zamora ,&nbsp;Martin Cañete ,&nbsp;Marina Medina ,&nbsp;María Paula Eguiguren ,&nbsp;Belen Marti ,&nbsp;Maria de los Ángeles Baridon ,&nbsp;Cristina Videla","doi":"10.1016/j.ram.2025.10.006","DOIUrl":"10.1016/j.ram.2025.10.006","url":null,"abstract":"<div><div>CMV is one of the main complications associated with transplantation, whether of solid organs or hematopoietic stem cells, and is related to an increase in post-transplant morbidity and mortality. Due to its significant impact, prevention is recommended, either through prophylaxis or other preventive therapies. The latter require viral monitoring of the patient, as viremia serves as a predictor of of the disease, allowing for an early detection and timely preventive treatment. The gold standard for viral monitoring is the quantitative determination of nucleic acids. Laboratories often face practical challenges regarding the type of sample to be used, its transport and storage conditions, the appropriate methods of nucleic acid extraction and quantification, the frequency of monitoring and establishment of cut-off points to ensure the accuracy and reproducibility of CMV viral load test results. The Argentine Society of Virology has formed a working group specifically dedicated to addressing these difficulties.</div><div>This document aims to provide recommendations on the aforementioned points based on the available scientific evidence and the expertise of the working group.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"58 1","pages":"Pages 83-100"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145638146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond human-centered surveillance: rethinking antimicrobial resistance monitoring in a One Health framework","authors":"Rafael Vignoli ,&nbsp;José Di Conza ,&nbsp;Nilton Lincopan","doi":"10.1016/j.ram.2026.01.002","DOIUrl":"10.1016/j.ram.2026.01.002","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"58 1","pages":"Pages 3-5"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147309598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracts from Argentinian native plants exhibit antifungal activity against multidrug-resistant Candida species","authors":"Alejandro N. Etchecopaz ,&nbsp;Lucia S. Di Ciaccio ,&nbsp;Katherine Hermida Alava ,&nbsp;Reneé H. Fortunato ,&nbsp;Adriana Salvat ,&nbsp;Alejandro D. Nusblat ,&nbsp;María L. Cuestas","doi":"10.1016/j.ram.2025.07.006","DOIUrl":"10.1016/j.ram.2025.07.006","url":null,"abstract":"<div><div>Infections caused by the genus <em>Candida</em> have acquired considerable significance in recent years due to the enhanced susceptibility of immunocompromised hosts. There have been increasing reports of multidrug resistance (MDR) in several <em>Candida</em> species, posing a major hurdle to antifungal therapy. Accordingly, exploring and developing novel anti-<em>Candida</em> agents has become a priority. In this study, we assessed the antifungal activity of seven methanolic extracts from the Argentinian native plants <em>Peltophorum dubium</em>, <em>Schinus areira</em>, <em>Parastrephia quadrangularis</em> and <em>Lantana balansae</em> against clinical isolates of different wild-type and MDR isolates of <em>Candida</em>. Synergism with fluconazole was also evaluated. All plant extracts showed antifungal activities against different <em>Candida</em> species, including MDR isolates such as <em>C. haemulonii</em>. Highly active extracts from these native plants provide promising sources of compounds for potentiating the antifungal effect of fluconazole. Further investigation of the chemical constituents of the extracts and their cytotoxicity is needed to develop plant-derived anti-<em>Candida</em> agents.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"58 1","pages":"Pages 31-35"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal degradation of PLA/PBAT bioplastics and plastic polymers by Aspergillus sp.","authors":"María Victoria Valerga Fernández ,&nbsp;Sofía Yasmín Utge Perri ,&nbsp;Roxana Paula Colombo ,&nbsp;Laura Moyano ,&nbsp;Alicia Margarita Godeas ,&nbsp;Vanesa Analía Silvani","doi":"10.1016/j.ram.2025.10.003","DOIUrl":"10.1016/j.ram.2025.10.003","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"58 1","pages":"Pages 133-134"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145550341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical management of Lancefieldella parvula bacteremia: A case study and review of the literature","authors":"Cynthia Wenglinski ,&nbsp;Delphine Martiny ,&nbsp;Michael Watchi ,&nbsp;Fabio Silvio Taccone ,&nbsp;Charles-Hervé Vacheron","doi":"10.1016/j.ram.2025.06.004","DOIUrl":"10.1016/j.ram.2025.06.004","url":null,"abstract":"<div><div>This report presented the case of a 55-year-old patient who was admitted to the intensive care unit following a ruptured bronchial arteriovenous malformation (AVM). The patient, with no significant medical history, experienced massive hemoptysis leading to cardiac arrest. After successful resuscitation and embolization of the AVM, the patient developed fever and acute respiratory distress syndrome, requiring deep sedation, muscle paralysis, and prone positioning. Blood culture sampled on the day of admission tested positive for <em>Lancefieldella parvula</em> on day 4. The patient received targeted antimicrobial therapy, resulting in a favorable hemodynamic and respiratory outcome. No source of the bacteremia was found. We reported the general management of this rare cause of bacteremia and provided a review of the existing literature.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"58 1","pages":"Pages 57-59"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}