Revista Argentina de microbiologia最新文献

{"title":"Wheat straw amendment to an oily sludge pretreated with ammonium persulfate and its impact on the indigenous mycobiota.","authors":"Natalia Andrea Di Clemente, Marina Peluffo, Ana Carolina Agnello, Deborah Colman, Norma Buceta, María Teresa Del Panno, Mario Carlos Nazareno Saparrat","doi":"10.1016/j.ram.2025.05.002","DOIUrl":"https://doi.org/10.1016/j.ram.2025.05.002","url":null,"abstract":"<p><p>Numerous studies have addressed the dynamics of bacterial communities in response to remediation strategies, while fungal communities, despite their potential as bioremediation agents, remain comparatively understudied. The aim of this work was to evaluate the impact of wheat straw amendment (WSA) on an oily sludge untreated and treated with ammonium persulfate on indigenous mycobiota after 60 days. Culture-independent 18S rRNA amplicon sequencing, microbial enzymatic activity and chemical parameters were analyzed. WSA on a preoxidized oily sludge promoted high total petroleum hydrocarbon (TPH) removal after 60 days, exhibiting the highest laccase activity. Fungal diversity and equitability were also recovered until reaching similar index values to those of the untreated microcosms. However, changes in fungal taxonomical groups were detected, with Eurotiales (93.4%) being replaced by Microascales (57.9%) and Sordariales (32.8%) at the end of the treatment. Our results suggest that inputs of easily assimilable organic matter in oily sludge might accelerate changes and replacement of fungal taxa, also affecting microbial colonization and, consequently, pollutant removal. These findings highlight the relevance of incorporating fungal dynamics into bioremediation strategies as a complementary approach to oily sludge treatment.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors associated with neonatal sepsis due to Serratia marcescens in a public hospital in Mexico: A case-control study.","authors":"Juan Carlos Lona-Reyes, Lucia Miramontes-Buiza, Luis Fernando Frías-Flores, Jose Angel Regla-Nava, Jesús González-Carmona, Juan Antonio Gallegos-Marín, Mariana Chávez-Rodríguez","doi":"10.1016/j.ram.2025.04.006","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.006","url":null,"abstract":"<p><p>This study aimed to identify factors associated with neonatal sepsis due to Serratia marcescens in a Neonatal Intensive Care Unit (NICU) in Mexico. A case-control study was conducted, matched by gestational age and birth weight. The analysis was performed using logistic regression. Seventy newborns with S. marcescens sepsis were identified; the median gestational age was 34.4 weeks, and the median birth weight was 1680g. Microbial isolates were identified in the bloodstream in 98.6% of cases, and 88.6% of these were diagnosed after 72h of life. Independent variables associated with infection were the presence of a central venous catheter (OR 5.17; 95% CI 1.57-17.1), mechanical ventilation (OR 3.23; 95% CI 1.18-8.87), and prior use of meropenem (OR 3.38; 95% CI 1.01-11.3). The case fatality rate of the infection was 32.9%.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial diversity investigation using 16S metagenomics in Tunisian patients with systemic lupus erythematosus.","authors":"Amira Messadi, Sameh Sayhi, Kais Ghedira, Chadia Zaouaoui, Bilel Arfaoui, Soumaya Khouikhi, Maha Rebai, Nour El Houda Guediche, Nadia Abdelhafidh, Bassem Louzir, Ajili Faida","doi":"10.1016/j.ram.2025.04.002","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.002","url":null,"abstract":"<p><p>Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease associated with significant morbidity and mortality. It is characterized by a loss of self-immune tolerance and autoantibody production, leading to multiple organ damage. Emerging investigations have confirmed the role of gut microbiota dysbiosis in patients with SLE, although the underlying mechanisms remain unclear to date. In this study, we aim to investigate the bacterial profile of SLE including phylum/class/genus relative abundance and diversity, to compare them with healthy controls and to study the correlation of relative abundance of different patterns with clinical/biological parameters. In this case-control study, the bacterial profile was investigated in 7 SLE patients and 7 healthy controls using 16S metagenomics clustering. The present study reported a low abundance of the class Bacilli (0.58% in SLE vs 1.26% in the controls), the genus Lactobacillus (0.43% vs 0.74%), as well as a higher abundance of the genera Gammaproteobacteria (2.37% vs 0.77%) and Escherichia-Shigella (2.04% vs 0.51%) in SLE samples compared to the controls (p<0.05). We also found an association between the class Betaproteobacteria (4.42% vs 1.57%) and the genus Faecalibacterium (11.34% vs 3.35%) and renal manifestations (p<0.05). The phylum Actinobacteria (0.21% vs 3.8%, p=0.036) and the genus Bifidobacterium levels were lower in active SLE compared to the healthy controls. This study is the first report on the gut microbiota of SLE and the first case-control study in Tunisia and North Africa. We obtained a particular profile of bacterial gut microbiota for the SLE group. We found a specific clustering when compared to the healthy controls.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of probiotic bacteria, phycocyanin and tarragon essential oil on the physiochemical, microbiological, and sensory properties of a mouth freshener tablet.","authors":"Fatemeh Habibi, Bahareh Nowruzi, Maryam Ghane","doi":"10.1016/j.ram.2025.04.004","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.004","url":null,"abstract":"<p><p>Microbial pigments are extensively used in industry as food coloring, dyes, and medicinal substances. This study aimed to develop a probiotic mouth freshener tablet containing PC, tarragon essential oil, and microencapsulated Lactobacillus plantarum and investigate its antimicrobial properties against Streptococcus mutans. First, cyanobacterium Spirulina sp. was cultured and PC extracted. Then, microbial suspension and microencapsulation of probiotic bacteria, tarragon essential oil extraction, as well as mouth freshener tablets were prepared. Four treatments (T) under Co: control sample, T₁, T₂, T₃, and T₄ contained 1% PC, 1% microcoating probiotic, 0.8% tarragon essential oil, and 1% PC+1% microcoating probiotic+0.8% tarragon essential oil, were prepared respectively. The results of the inhibition zone diameter of samples also indicated that the highest and lowest sensitivity were observed in T₄ and T₁, respectively. Based on the results, the addition of PC and tarragon essential oil significantly increased the antioxidant activity of the samples (p<0.05). The results indicated that the release rate of probiotic bacteria under gastric conditions in T₄ was lower compared to that in treatment T₂ (p<0.05). Sensory evaluation results showed that the highest values were observed in T₃ and T₄ (p<0.05). CONCLUSION: Overall, it can be concluded that the prepared mouth freshener tablets (T₄) can be effective agents for oral disinfection and the promotion of public health.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mosquito-borne pathogens … Now Rickettsias?","authors":"Silvina Goenaga, Estefania Raquel Boaglio, Lucia Victoria Martinez, Juliana Patricia Sanchez","doi":"10.1016/j.ram.2025.03.002","DOIUrl":"https://doi.org/10.1016/j.ram.2025.03.002","url":null,"abstract":"<p><p>In the last few decades, the emergence of several infectious diseases has increased. Mosquitoes are the most important vectors due to their role in transmitting several pathogens, such as dengue, chikungunya and Plasmodium spp. Recently, mosquitoes have been suspected of transmitting Rickettsias, another emerging pathogen. However, their role in the transmission of this agent remains unclear. In this study, we report the detection of a Rickettsia spp. in Coquillettidia venezuelensis mosquitoes collected from Corrientes Province, Argentina. The phylogenetic analyses show that this Rickettsia is related to Rickettsia felis, and the ompB sequence shows high homology with Rickettsia tillamookensis. The evidence of the detection of Rickettsia in mosquitoes shows that several infectious diseases could be transmitted by mosquitoes, and the interaction between them is important to investigate. More studies should be done in order to analyze the impact on public health.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition in phyla from breast milk: Effect of the mode of delivery.","authors":"Shiva Aslani, Seyedeh Neda Mousavi, Atefeh Kazemi Robati, Siamak Heidarzadeh, Somaye Abdollahi Sabet","doi":"10.1016/j.ram.2025.04.001","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.001","url":null,"abstract":"<p><p>Breast milk is considered as a living ecosystem. Maternal, environmental and neonatal factors affect milk bacterial composition. The aim of present study was to assess the phyla from breast milk of mothers with vaginal delivery compared to the cesarean section. In this single-center case-control study, sixty women were participated. Half of them had vaginal delivery and others experienced cesarean section. The breast milk samples were collected three months after delivery for the DNA extraction to measure Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria using quantitative real time chain polymerase reaction. Daily intake of calories, protein, fat, carbohydrate and fiber did not differ significantly between the two groups. The proportion of Bacteroidetes, Firmicutes and Proteobacteria were significantly higher in milk of mothers with a cesarean section than the vaginal delivery (p=0.03, p=0.02 and p=0.042). Similarly, the Firmicutes to Bacteroides ratio was significantly increased (p=0.02). The Actinobacteria population was significantly higher in milk of vaginally-delivered mothers who had male infant than females (p=0.015). Breast milk of mothers with cesarean section showed alterations in the main bacterial phyla population compared to the vaginal delivery. Moreover, our results suggest that the sex of infant is an effective factor on some phyla quantity.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity assessment of Bacillus thuringiensis strains for the control of the lesser mealworm beetle Alphitobius diaperinus (Coleoptera: Tenebrionidae).","authors":"Melisa P Pérez, Graciela B Benintende, Diego H Sauka","doi":"10.1016/j.ram.2025.03.003","DOIUrl":"https://doi.org/10.1016/j.ram.2025.03.003","url":null,"abstract":"<p><p>This study addresses the pervasive challenge of lesser mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae) infestations in poultry farming. Our aim was to select toxic Bacillus thuringiensis strains against A. diaperinus larvae and determine the fraction(s) within the bacterial cultures harboring the active metabolites responsible for this insecticidal activity. Among the 41 strains evaluated, the Argentine strain INTA Mo4-4 showed the highest toxicity. Bioassays revealed that the main virulence factors reside within the spore-crystal pellet associated with the Cry protein. These results set the basis for the development of a targeted bioinsecticide. Further research is needed to elucidate the underlying mechanisms and validate these strategies in field trials. By harnessing B. thuringiensis-based bioinsecticides, we offer a sustainable solution to mitigate A. diaperinus infestations.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of intestinal microbiota diversity in children with non-organic anorexia.","authors":"Wei Lu, Zong-Long Li, De-Yong Xu, Guo-Ping Yu","doi":"10.1016/j.ram.2025.02.001","DOIUrl":"https://doi.org/10.1016/j.ram.2025.02.001","url":null,"abstract":"<p><p>The pathogenesis of non-organic anorexia in children is not clear. This study intends to analyze intestinal bacteria to provide a relevant theoretical basis for the clinical rational selection of microecological agents. In the present study, children with non-organic anorexia were included in the anorexia group and normal healthy children in the control group. Stool samples were collected for the bioinformatics analysis after PCR and high-throughput sequencing. The results showed that the Ace, Chao, and Shannon indexes in the anorexia group were higher than those in the control group, while the Simpson index in the control group was lower than in the anorexia group. There were 14 taxa in the anorexia group and 11 taxa in the healthy control group at the phylum level, and 193 taxa in the anorexia group and 180 in the control group at the genus level. The dominant bacteria at the phylum level of the two groups were the same, while there were 16 dominant bacteria taxa in the anorexia group and 17 in the control group at the genus level. The ratio of percentage abundance of Bacteroidetes to that of Firmicutes (the B/F index) in the anorexia group was higher than in the control group. The abundance of Bacteroidetes in the anorexia group was higher than that in the control group, and the abundance of Actinomycetes in the control group was higher than that in the anorexia group. There were significant differences in 14 dominant genera between the two groups at the genus classification level. The LEfSe multilevel species difference analysis showed that at the phylum level, the significant influential bacterial taxa in the anorexia group were Bacteroidetes and Actinobacteria in the control group. At the genus level, the significant influential bacterial taxa in the anorexia group were Bacteroides, Faecalibacterium, and Subdoligranulum, and Bifidobacterium, Blautia, Streptococcus, Lachnoclostridium, and Erysipelatoclostridium in the control group. We conclude that the increase in Bacteroides abundance or in the B/F index and the reduction in Bifidobacterium abundance were related to the pathogenesis of anorexia.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospective evaluation of Leptospira spp. infection in goats maintained in field conditions in the Caatinga biome, Brazil.","authors":"Mayla de Lisbôa Padilha, Lídio Ricardo Bezerra de Melo, Clécio Henrique Limeira, Nathália Maria de Andrade Magalhães, Maria Luana Cristiny Rodrigues Silva, Rafael Rodrigues Soares, Clebert José Alves, Severino Silvano Dos Santos Higino, Carolina de Sousa Américo Batista Santos, Sérgio Santos de Azevedo","doi":"10.1016/j.ram.2025.03.001","DOIUrl":"https://doi.org/10.1016/j.ram.2025.03.001","url":null,"abstract":"<p><p>The Caatinga biome occurs only in Brazil; however, there is no survey on leptospirosis using direct and indirect diagnostic tests in this region with goats maintained in field conditions. We conducted a prospective survey with paired sampling to evaluate the importance of carrier goats in the maintenance of disease. Based on sampling calculation, 60 goats (30 confined and 30 extensively reared) were randomly selected and monitored for three months during the rainy season with paired monthly biological sample collections. The animals underwent urine and vaginal fluid collection for microbiological and molecular diagnosis, and serum samples for serological diagnosis using the microscopic agglutination test (MAT). Overall, 45 (75%) animals were positive in at least one diagnostic method. Anti-Leptospira spp. antibodies were detected in all three sampling moments in 43 (71.7%) animals, antibody titers varied from 25 to 800, and most frequent serogroups were Australis in the 1st and 2nd blood collections (66.7% and 40.7%, respectively) and Cynopteri in the 3rd collection (52.6%). None of the animals tested positive in the microbiological diagnosis or the vaginal fluid PCR; however, five (16.7%) animals were positive in the urine PCR only in the confined group in the first collection. Two DNA urine samples were sequenced, demonstrating 99% similarity with Leptospira interrogans. Different diagnostic techniques for leptospirosis in goats raised under Caatinga biome field conditions are suggested, as well as further studies over a longer period with monthly collections to better understand the prevalence of Leptospira spp. and its variation over time.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall structure and its influence on aflatoxin B1 binding","authors":"Carina Pereyra ,&nbsp;María del Pilar Monge ,&nbsp;Silvestre Bongiovanni ,&nbsp;Andrea Cristofolini ,&nbsp;Sergio Campos ,&nbsp;Lilia Cavaglieri","doi":"10.1016/j.ram.2024.07.004","DOIUrl":"10.1016/j.ram.2024.07.004","url":null,"abstract":"<div><div>The aim of this study was to determine the impact of <em>Kluyveromyces marxianus</em> VM004 culture conditions on the cell wall (CW) structure and its influence on aflatoxin B₁ binding. The yeast was inoculated into two types of culture media: yeast extract-peptone-dextrose (YPD) broth and dried distiller's grains with solubles (DDG). The CW was extracted from the biomass produced in these media. AFB₁ (150<!--> <!-->ng/ml) adsorption tests using the biomass (1<!--> <!-->×<!--> <!-->10⁷ <!-->cells/ml) and the CW (0.001<!--> <!-->g) were performed at pH 2 and pH 8. Transmission electron microscopy (TEM) evaluated the CW thickness, and infrared spectroscopy (IR) determined the CW composition. Biomass production in YPD was higher than that in DDG. Cell diameter (μm) and CW thickness (μm) increased in the DDG medium. The CW percentage obtained in DDG was higher than that in YPD. The absorbance of carbohydrates by IR was higher in YPD. pH influenced AFB₁ adsorption, which was lower at pH 8. The proportion of β-glucan and chitin present in CW was higher in the YPD medium. The IR method allowed to study the CW carbohydrate variation under the influence of these carbon sources. In conclusion, the culture media composition influenced the β-glucan and chitin composition and consequently, AFB₁ adsorption.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 89-97"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}