Revista Argentina de microbiologia最新文献

Phenotypic and genomic surveillance of invasive Neisseria meningitidis isolates in Argentina (2015-2022): Clonal structure and vaccine implications. 阿根廷侵袭性脑膜炎奈瑟菌分离株的表型和基因组监测（2015-2022）：克隆结构和疫苗意义

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-07-10 DOI: 10.1016/j.ram.2025.04.005

Adriana Efron, Luciana Moreira, Federico Lorenzo, Denise De Belder, María Moscoloni, Alejandra Corso, Mauricio Santos, Tomás Poklepovich, Josefina Campos, Paula Gagetti, María Sol Haim

{"title":"Phenotypic and genomic surveillance of invasive Neisseria meningitidis isolates in Argentina (2015-2022): Clonal structure and vaccine implications.","authors":"Adriana Efron, Luciana Moreira, Federico Lorenzo, Denise De Belder, María Moscoloni, Alejandra Corso, Mauricio Santos, Tomás Poklepovich, Josefina Campos, Paula Gagetti, María Sol Haim","doi":"10.1016/j.ram.2025.04.005","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.005","url":null,"abstract":"Invasive meningococcal disease is associated with high fatality rates and disabling sequelae. The aims of this study were to describe the phenotypic and genotypic characteristics of invasive Neisseria meningitidis isolates in Argentina, determine the genomic population structure and evaluate the potential coverage of vaccines targeting N. meningitidis serogroup B (MenB). During the period 2015-2022, a total of 444 isolates of N. meningitidis causing invasive disease were assessed through laboratory surveillance; 344 isolates were available for whole genome sequencing characterization. The presence of antimicrobial resistance genes, and the distribution of clonal complexes and vaccine antigens were analyzed. MenB was the most frequent serogroup (53.2%), followed by MenW (32.0%). Most isolates were susceptible to the antimicrobial agents tested; however, 56.7% exhibited intermediate resistance to penicillin. MenB showed wide genetic diversity and was mainly associated with ST-865 cc, ST-35 cc, ST-41/44 cc, and ST-32 cc. Unlike other countries, in Argentina, ST-865 cc was one of the major clonal complexes associated with MenB, MenW was only associated with ST-11 cc. To study the potential coverage of vaccines targeting MenB, we used the MenDeVAR (Meningococcal Deduced Vaccine Antigen Reactivity) index, which provides information on the presence and possible immunological cross-reactivity of the different vaccine antigenic variants. MenDeVar showed 19.3% vaccine reactivity for 4CMenB and 40.0% for the bivalent vaccine. Laboratory surveillance is essential for generating evidence-based decisions for updating vaccination strategies.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic resistance to infectious diseases: A complementary tool to antimicrobial drugs in livestock? 对传染病的遗传抗性：家畜抗微生物药物的补充工具？

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-07-07 DOI: 10.1016/j.ram.2025.06.001

Carlos A Rossetti, Ursula A Rossi

引用次数: 0

Analysis of Escherichia coli isolated from minced meat: Implications for public health in Tierra del Fuego, Argentina. 从肉糜中分离的大肠杆菌分析：对阿根廷火地岛公共卫生的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-06-19 DOI: 10.1016/j.ram.2025.05.001

Elisa Crespi, Bárbara Ghiglione, Ximena Blanco Crivelli, Cecilia Cundon, Roque Figueroa Espinosa, Pedro Penzotti, Ezequiel Nuske, Alicia Broglio, Luis Ambros, María Valeria Rumi, Gabriela Albarellos, José Di Conza, Adriana Bentancor

{"title":"Analysis of Escherichia coli isolated from minced meat: Implications for public health in Tierra del Fuego, Argentina.","authors":"Elisa Crespi, Bárbara Ghiglione, Ximena Blanco Crivelli, Cecilia Cundon, Roque Figueroa Espinosa, Pedro Penzotti, Ezequiel Nuske, Alicia Broglio, Luis Ambros, María Valeria Rumi, Gabriela Albarellos, José Di Conza, Adriana Bentancor","doi":"10.1016/j.ram.2025.05.001","DOIUrl":"10.1016/j.ram.2025.05.001","url":null,"abstract":"Escherichia coli contamination in minced meat poses a significant public health concern and may serve as a reservoir for antimicrobial-resistant strains. This study investigated the presence of E. coli resistant to ciprofloxacin and/or gentamicin in minced meat samples from Tierra del Fuego, Argentina. Resistant E. coli isolates were identified using MALDI-TOF. A total of 109 samples were analyzed, yielding 97 E. coli isolates (23 from AMC-CIP and 74 from AMC-GEN). Twenty-three isolates carried the ant(2″) gene, ten the ant(3″) gene, and one the blaCTX-M gene. Resistance was observed to gentamicin (6/25), streptomycin (1/25), ceftazidime (1/25), and cefotaxime (4/25). Eleven isolates carried the aggR gene, and another three were identified as biofilm producers. Although phenotypic resistance was low, we detected genetic potential for resistance which, combined with biofilm formation, represents a risk to food safety.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wheat straw amendment to an oily sludge pretreated with ammonium persulfate and its impact on the indigenous mycobiota. 麦秸改性过硫酸铵预处理含油污泥及其对本地真菌群的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-06-18 DOI: 10.1016/j.ram.2025.05.002

Natalia Andrea Di Clemente, Marina Peluffo, Ana Carolina Agnello, Deborah Colman, Norma Buceta, María Teresa Del Panno, Mario Carlos Nazareno Saparrat

{"title":"Wheat straw amendment to an oily sludge pretreated with ammonium persulfate and its impact on the indigenous mycobiota.","authors":"Natalia Andrea Di Clemente, Marina Peluffo, Ana Carolina Agnello, Deborah Colman, Norma Buceta, María Teresa Del Panno, Mario Carlos Nazareno Saparrat","doi":"10.1016/j.ram.2025.05.002","DOIUrl":"10.1016/j.ram.2025.05.002","url":null,"abstract":"Numerous studies have addressed the dynamics of bacterial communities in response to remediation strategies, while fungal communities, despite their potential as bioremediation agents, remain comparatively understudied. The aim of this work was to evaluate the impact of wheat straw amendment (WSA) on an oily sludge untreated and treated with ammonium persulfate on indigenous mycobiota after 60 days. Culture-independent 18S rRNA amplicon sequencing, microbial enzymatic activity and chemical parameters were analyzed. WSA on a preoxidized oily sludge promoted high total petroleum hydrocarbon (TPH) removal after 60 days, exhibiting the highest laccase activity. Fungal diversity and equitability were also recovered until reaching similar index values to those of the untreated microcosms. However, changes in fungal taxonomical groups were detected, with Eurotiales (93.4%) being replaced by Microascales (57.9%) and Sordariales (32.8%) at the end of the treatment. Our results suggest that inputs of easily assimilable organic matter in oily sludge might accelerate changes and replacement of fungal taxa, also affecting microbial colonization and, consequently, pollutant removal. These findings highlight the relevance of incorporating fungal dynamics into bioremediation strategies as a complementary approach to oily sludge treatment.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk factors associated with neonatal sepsis due to Serratia marcescens in a public hospital in Mexico: A case-control study. 墨西哥公立医院粘质沙雷菌所致新生儿败血症的相关危险因素：一项病例对照研究

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-06-11 DOI: 10.1016/j.ram.2025.04.006

Juan Carlos Lona-Reyes, Lucia Miramontes-Buiza, Luis Fernando Frías-Flores, Jose Angel Regla-Nava, Jesús González-Carmona, Juan Antonio Gallegos-Marín, Mariana Chávez-Rodríguez

引用次数: 0

Microbial diversity investigation using 16S metagenomics in Tunisian patients with systemic lupus erythematosus. 突尼斯系统性红斑狼疮患者16S宏基因组学微生物多样性研究。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-06-03 DOI: 10.1016/j.ram.2025.04.002

Amira Messadi, Sameh Sayhi, Kais Ghedira, Chadia Zaouaoui, Bilel Arfaoui, Soumaya Khouikhi, Maha Rebai, Nour El Houda Guediche, Nadia Abdelhafidh, Bassem Louzir, Ajili Faida

{"title":"Microbial diversity investigation using 16S metagenomics in Tunisian patients with systemic lupus erythematosus.","authors":"Amira Messadi, Sameh Sayhi, Kais Ghedira, Chadia Zaouaoui, Bilel Arfaoui, Soumaya Khouikhi, Maha Rebai, Nour El Houda Guediche, Nadia Abdelhafidh, Bassem Louzir, Ajili Faida","doi":"10.1016/j.ram.2025.04.002","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.002","url":null,"abstract":"Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease associated with significant morbidity and mortality. It is characterized by a loss of self-immune tolerance and autoantibody production, leading to multiple organ damage. Emerging investigations have confirmed the role of gut microbiota dysbiosis in patients with SLE, although the underlying mechanisms remain unclear to date. In this study, we aim to investigate the bacterial profile of SLE including phylum/class/genus relative abundance and diversity, to compare them with healthy controls and to study the correlation of relative abundance of different patterns with clinical/biological parameters. In this case-control study, the bacterial profile was investigated in 7 SLE patients and 7 healthy controls using 16S metagenomics clustering. The present study reported a low abundance of the class Bacilli (0.58% in SLE vs 1.26% in the controls), the genus Lactobacillus (0.43% vs 0.74%), as well as a higher abundance of the genera Gammaproteobacteria (2.37% vs 0.77%) and Escherichia-Shigella (2.04% vs 0.51%) in SLE samples compared to the controls (p<0.05). We also found an association between the class Betaproteobacteria (4.42% vs 1.57%) and the genus Faecalibacterium (11.34% vs 3.35%) and renal manifestations (p<0.05). The phylum Actinobacteria (0.21% vs 3.8%, p=0.036) and the genus Bifidobacterium levels were lower in active SLE compared to the healthy controls. This study is the first report on the gut microbiota of SLE and the first case-control study in Tunisia and North Africa. We obtained a particular profile of bacterial gut microbiota for the SLE group. We found a specific clustering when compared to the healthy controls.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of probiotic bacteria, phycocyanin and tarragon essential oil on the physiochemical, microbiological, and sensory properties of a mouth freshener tablet. 益生菌、藻蓝蛋白和龙蒿精油对口腔清新片理化、微生物学和感官特性的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-05-28 DOI: 10.1016/j.ram.2025.04.004

Fatemeh Habibi, Bahareh Nowruzi, Maryam Ghane

{"title":"Effect of probiotic bacteria, phycocyanin and tarragon essential oil on the physiochemical, microbiological, and sensory properties of a mouth freshener tablet.","authors":"Fatemeh Habibi, Bahareh Nowruzi, Maryam Ghane","doi":"10.1016/j.ram.2025.04.004","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.004","url":null,"abstract":"Microbial pigments are extensively used in industry as food coloring, dyes, and medicinal substances. This study aimed to develop a probiotic mouth freshener tablet containing PC, tarragon essential oil, and microencapsulated Lactobacillus plantarum and investigate its antimicrobial properties against Streptococcus mutans. First, cyanobacterium Spirulina sp. was cultured and PC extracted. Then, microbial suspension and microencapsulation of probiotic bacteria, tarragon essential oil extraction, as well as mouth freshener tablets were prepared. Four treatments (T) under Co: control sample, T1, T2, T3, and T4 contained 1% PC, 1% microcoating probiotic, 0.8% tarragon essential oil, and 1% PC+1% microcoating probiotic+0.8% tarragon essential oil, were prepared respectively. The results of the inhibition zone diameter of samples also indicated that the highest and lowest sensitivity were observed in T4 and T1, respectively. Based on the results, the addition of PC and tarragon essential oil significantly increased the antioxidant activity of the samples (p<0.05). The results indicated that the release rate of probiotic bacteria under gastric conditions in T4 was lower compared to that in treatment T2 (p<0.05). Sensory evaluation results showed that the highest values were observed in T3 and T4 (p<0.05). CONCLUSION: Overall, it can be concluded that the prepared mouth freshener tablets (T4) can be effective agents for oral disinfection and the promotion of public health.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mosquito-borne pathogens … Now Rickettsias? 蚊子传播的病原体…现在是立克次体？

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-05-26 DOI: 10.1016/j.ram.2025.03.002

Silvina Goenaga, Estefania Raquel Boaglio, Lucia Victoria Martinez, Juliana Patricia Sanchez

引用次数: 0

Composition in phyla from breast milk: Effect of the mode of delivery. 母乳中细菌门的组成：分娩方式的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-05-22 DOI: 10.1016/j.ram.2025.04.001

Shiva Aslani, Seyedeh Neda Mousavi, Atefeh Kazemi Robati, Siamak Heidarzadeh, Somaye Abdollahi Sabet

{"title":"Composition in phyla from breast milk: Effect of the mode of delivery.","authors":"Shiva Aslani, Seyedeh Neda Mousavi, Atefeh Kazemi Robati, Siamak Heidarzadeh, Somaye Abdollahi Sabet","doi":"10.1016/j.ram.2025.04.001","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.001","url":null,"abstract":"Breast milk is considered as a living ecosystem. Maternal, environmental and neonatal factors affect milk bacterial composition. The aim of present study was to assess the phyla from breast milk of mothers with vaginal delivery compared to the cesarean section. In this single-center case-control study, sixty women were participated. Half of them had vaginal delivery and others experienced cesarean section. The breast milk samples were collected three months after delivery for the DNA extraction to measure Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria using quantitative real time chain polymerase reaction. Daily intake of calories, protein, fat, carbohydrate and fiber did not differ significantly between the two groups. The proportion of Bacteroidetes, Firmicutes and Proteobacteria were significantly higher in milk of mothers with a cesarean section than the vaginal delivery (p=0.03, p=0.02 and p=0.042). Similarly, the Firmicutes to Bacteroides ratio was significantly increased (p=0.02). The Actinobacteria population was significantly higher in milk of vaginally-delivered mothers who had male infant than females (p=0.015). Breast milk of mothers with cesarean section showed alterations in the main bacterial phyla population compared to the vaginal delivery. Moreover, our results suggest that the sex of infant is an effective factor on some phyla quantity.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicity assessment of Bacillus thuringiensis strains for the control of the lesser mealworm beetle Alphitobius diaperinus (Coleoptera: Tenebrionidae). 苏云金芽孢杆菌菌株对小粉虫甲（鞘翅目：拟粉虫科）的毒力评价。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-05-22 DOI: 10.1016/j.ram.2025.03.003

Melisa P Pérez, Graciela B Benintende, Diego H Sauka

引用次数: 0