Revista Argentina de microbiologia最新文献

{"title":"Diversity of hypervirulent Klebsiella pneumoniae clones causing cryptogenic liver abscesses and metastatic complications in Argentina.","authors":"Esteban C Nannini, Matías Lahitte, Pablo Scapellato, Corina Nemirosvky, Marcelo Zylberman, Andrea Vila, Viviana Rodríguez, Roman Zucchi, Analia Mykietiuk, Valeria David, Adriana Limansky, Patricia Marchiaro, Mariángel Rinaudo","doi":"10.1016/j.ram.2024.11.006","DOIUrl":"https://doi.org/10.1016/j.ram.2024.11.006","url":null,"abstract":"<p><p>Cryptogenic liver abscesses (CLA) caused by hypervirulent Klebsiella pneumoniae (hvKP) strains are emerging in Western countries. The aim of the study was to describe the clinical characteristics of patients from Argentina with hvKP-related CLA as well as the molecular analysis of isolated strains. A retrospective chart review of 15 patients hospitalized in 8 hospitals of Argentina between October 2015 and November 2018 was performed. PCR assays for genes associated with capsular and multilocus sequence typing (MLST) determination and virulence factors were conducted in 8 hvKP isolates from these patients. We found that the mean age of patients was 60 years, 73% of them were men and 40% suffered from diabetes. Bacteremia was detected in 60% of them and 73% had ≥1 metastatic foci of infection. There were no in-hospital deaths, but two patients with endophthalmitis required eye enucleation. Of the 8 studied isolates, 4 belonged to K1 and 4 to K2 serotypes, with the rpmA and iroB genes being present in all of them, and isolates 7 and 5 also harboring the iucA and the rmpA2 genes, respectively. MSLT analysis showed that most of the K1 serotypes belonged to ST23 while a diverse MLST pattern was observed among the K2 strains. In addition, the four hvKP strains associated with metastatic complications and belonging to three distinct sequence types, exhibited the rpmA, iroB and iuc virulence genes. We were able to demonstrate important morbidity associated with this syndrome, a significant diversity in the hvKP clones causing CLA in Argentina, and the potential utility of the rpmA and iroB genes as predictors of virulence.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of DNA extraction method in gut fungal community assessment.","authors":"Néstor D Portela, Cristian Mena, Mauricio G Martín, Verónica L Burstein, Laura S Chiapello, Susana A Pesoa","doi":"10.1016/j.ram.2024.12.005","DOIUrl":"https://doi.org/10.1016/j.ram.2024.12.005","url":null,"abstract":"<p><p>Understanding the gut mycobiota composition and its impact on health requires reliable methods for fungal community assessment. This study explores the influence of DNA extraction methods in GM analysis. Three protocols were evaluated: Qiagen DNeasy blood and tissue kit with mechanical glass bead lysis (DNgb), Thermofisher MagMax Microbiome ultra-nucleic isolation kit automated method (MM), and MM combined with glass beads lysis (MMgb). Fecal samples from healthy volunteers were collected, DNA extracted and ITS2 amplicon library preparation and sequencing performed. Results showed that DNA yields did not significantly differ among methods and the addition of glass bead beating favored the recovery of DNA more appropriate for fungal analysis. Beta diversity revealed distinct clusters, with MMgb showing the most pronounced variation in mycobiota composition, exposing particularly the low abundance taxa. LEfSe analysis identified significant differences in the abundance of fungal species among the extraction methods. Samples extracted with bead beating were enriched in filamentous species, while those without this step showed higher relative abundance of yeast fungi. This study underscores the importance of selecting appropriate DNA extraction methods for accurate characterization of the gut mycobiota, emphasizing the need for standardized methodologies to ensure reproducibility and reliability in microbial data acquisition.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Report of a field control experience of bovine viral diarrhea virus in 2 dairy farms of Argentina applying management, diagnostic, and vaccination tools].","authors":"Rodrigo Pereyra, Fernando Martino, Mariángeles Castillo, Juan Manuel Sala, Lucas José Barone, Claudio Paolazzi, Alejandra Victoria Capozzo","doi":"10.1016/j.ram.2024.12.001","DOIUrl":"https://doi.org/10.1016/j.ram.2024.12.001","url":null,"abstract":"<p><p>Bovine viral diarrhea virus (BVDV) causes significant economic losses in the international livestock industry and in Argentina, where it circulates at high prevalence. Under high prevalence conditions, BVDV infections are controlled through vaccination once persistently infected animals are identified and segregated. This study evaluated the feasibility of controlling BVDV circulation under field conditions by combining diagnosis, management measures, and vaccination in 2 dairy farms in the province of Santa Fe. Commercial ELISAs were used for the detection of the NS3 (P80) protein or antibodies against this protein as well as an RT-nested PCR for the detection of the viral genome, and viral seroneutralization to assess vaccine efficacy. The average seroprevalence of the farms was 58.4%, with a persistently infected animal rate of 2.4%. After segregating the persistently infected animals and vaccinating them with a commercial combined vaccine containing inactivated BVDV, abortion rates significantly decreased (p<0.05) in farm 1 (from 20.5 to 11.6%) and in farm 2 (from 34 to 23.4%) during the second year of the control strategy. Conception rates increased from 29 to 33% in farm 1 during the first year, while in farm 2, the increase was 7 points during the second year. This methodology achieved conditions in which BVDV ceased to circulate, constituting the first controlled report on BVDV management in dairy farms using tools available to producers in Argentina.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized DNA extraction protocol for Staphylococcus aureus strains utilizing liquid nitrogen.","authors":"Mariana B Galeano, Stefania A Robaldi, Tania B Gordillo, Martiniano M Ricardi, Pablo M Cassanelli, Rosana O Pereda, Maria Mercedes Palomino, Paula Maria Tribelli","doi":"10.1016/j.ram.2024.12.006","DOIUrl":"https://doi.org/10.1016/j.ram.2024.12.006","url":null,"abstract":"<p><p>DNA extraction is crucial for conducting procedures, such as whole-genome sequencing, which demand methods that are reproducible and cost-effective. Lysing Staphylococcus aureus cells is particularly challenging due to their peptidoglycan layer that is resistant to common treatments. Traditional methods involve costly enzymatic lysis using lysostaphin. Here, we developed a novel approach for lysis utilizing liquid nitrogen and mechanical disruption in a mortar. DNA from S. aureus USA300 and related clinical isolates were purified using phenol-chloroform extraction followed by precipitation. The integrity and purity of DNA were confirmed, obtaining suitable concentration and purity for various molecular biology techniques. The quality of the employed DNA was validated by amplifying fragments of different genes using PCR. This method circumvents lysostaphin, yielding DNA that is suitable for use in other techniques.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endemic coronavirus in children and adults with acute respiratory infection before the COVID-19 pandemic.","authors":"Raquel Jara, Dafne Santos, Noelia Soledad Reyes, Eliana Hermida, Alejandro Seoane, Martin Ypas, Gisela Andres, Marcela Echavarría","doi":"10.1016/j.ram.2024.11.003","DOIUrl":"https://doi.org/10.1016/j.ram.2024.11.003","url":null,"abstract":"<p><p>Acute respiratory infection (ARI) is one of the principal causes of morbidity worldwide, with respiratory viruses being common etiological agents. Among them, endemic human coronaviruses (hCoVs) including CoV-229E, CoV-OC43, CoV-NL63, and CoV-HKU1 can cause mild ARI but are usually not evaluated in the clinical setting. The aim of this work was to determine the prevalence of all respiratory pathogens, with the focus placed on endemic hCoVs in the pre-pandemic period. Circulating species, clinical associations and coinfections with other respiratory pathogens were evaluated in 510 immunocompetent patients (children and adults) with ARI using the FilmArray® Respiratory Panel (BioFire/bioMérieux). A total of 399 children (252 outpatients and 147 hospitalized) and 111 adult outpatients were enrolled in the pre-pandemic period (2008-2010 and 2016). Endemic hCoVs were the third and fifth more frequently detected viruses among adults and outpatient children, respectively, with an overall frequency close to 10%. The most prevalent species were CoV-OC43 (42.8%) and CoV-HKU1 (40.5%), followed by CoV-NL63 (19.0%) and CoV-229E (4.8%). Tachypnea, wheezing and chest indrawing were more frequent in hospitalized children compared to outpatients. All adult patients presented with symptoms of a common cold. Endemic hCoVs were detected year-round, primarily between June and November. Our results highlight their clinical relevance, and the need to include endemic hCoVs in routine screening. In the post-pandemic period, further long-term surveillance is needed for understanding the epidemiology of endemic hCoVs and their evolution, as a tool to anticipate the possible emergence of new species.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intervención educativa como estrategia de prevención de parasitosis intestinales y valoración del estado enteroparasitológico en centros de desarrollo infantil de la ciudad de Santa Fe, Argentina","authors":"César Ernesto Gutiérrez ,&nbsp;María Agustina Jaras Horno ,&nbsp;Carolina Aro ,&nbsp;Andrea Florencia Gómez Colussi ,&nbsp;María Belén Cheirano ,&nbsp;Érica Soledad Rodríguez ,&nbsp;María Verónica Vera Garate ,&nbsp;Guillermo García Effron ,&nbsp;Sergio Adrián Guerrero","doi":"10.1016/j.ram.2024.07.002","DOIUrl":"10.1016/j.ram.2024.07.002","url":null,"abstract":"<div><div>Intestinal parasitic diseases constitute a cosmopolitan public health concern, with greater prevalence in developing countries, and mainly affecting children. The aim of this study was to develop an educational intervention aimed at mothers/guardians of children attending three child development centers (CDI) in Santa Fe, Argentina, during 2018. An educational intervention was conducted using a descriptive, quasi-experimental design, with pre- and post-intervention assessment. This intervention included 36 mothers, and was carried out in three stages: diagnosis, intervention and final evaluation. Simultaneously, a coproparasitological study was performed on 48 children under 5 years of age, who were assisted in the CDI, with the aim to understand and address the parasitic infections prevalent in the child population. It was possible to compare the correct answers before and after the educational intervention, observing a statistically significant increase (<em>p</em> <!-->=<!--> <!-->0.008742) in the average number of correct answers. Enteroparasites were identified in 54% of the population of children analyzed, 10% were biparasitized and 6% had more than two parasitic species. The most frequently detected parasites were <em>Blastocystis</em> spp., <em>Giardia intestinalis</em> and <em>Ascaris lumbricoides</em>. The educational intervention was positive, resulting in an improvement in the level of knowledge related to intestinal parasitosis and its prevention. This educational intervention experience highlights the importance of ongoing education in promoting a healthy lifestyle and preventing parasitosis in vulnerable populations.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 8-13"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bartolinitis por Neisseria meningitidis: caso clínico","authors":"Elena Berardinelli ,&nbsp;Marcela Sokol ,&nbsp;Laura Dufranc ,&nbsp;Vanina Diaz ,&nbsp;Viviana Eiras ,&nbsp;Ariel Gianecini ,&nbsp;Patricia Galarza ,&nbsp;Paula Gagetti ,&nbsp;Federico Lorenzo ,&nbsp;Adriana Efron","doi":"10.1016/j.ram.2024.10.004","DOIUrl":"10.1016/j.ram.2024.10.004","url":null,"abstract":"<div><div>Bartholinitis is the inflammation and infection of the Bartholin's glands that results from the accumulation of mucus in their ducts, the most frequent causal microorganisms being anaerobic and aerobic bacteria and those responsible for sexually transmitted infections. Those caused by agents not belonging to the genital microbiota are less frequent. Likewise, in most cases the diagnosis is clinical. Here, we describe the case of a 42-year-old heterosexual woman with a Bartholin's gland abscess caused by <em>Neisseria meningitidis</em>, isolated through culture and identified by VITEK®2. For molecular characterization and subtyping, the sequence type and clonal complex were determined using the multiple locus sequence typing technique. The antigenic profile was studied by amplification and sequencing of the genes coding for PorA, fHbp, NHBA and NadA and the susceptibility profile was assessed by MIC. Molecular diagnosis led to the confirmation of <em>N. meningitidis</em> as the pathogen responsible for bartholinitis.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 63-65"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced antimicrobial effects of carvacrol against methicillin-resistant Staphylococcus aureus strains using niosome formulations","authors":"Ronak Bakhtiari ,&nbsp;Maliheh Shiri ,&nbsp;Mohammad Reza Mohammadi ,&nbsp;Mohammad Reza Pourmand ,&nbsp;Amir Mirzaie ,&nbsp;Zahra Taghiabadi","doi":"10.1016/j.ram.2024.10.001","DOIUrl":"10.1016/j.ram.2024.10.001","url":null,"abstract":"<div><div>Methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) causes a wide range of infections and contributes to elevated morbidity, mortality, and healthcare costs. Herbal compounds combined with drug delivery systems could be an effective alternative option for treating resistant bacteria. This study evaluates the antimicrobial prowess of carvacrol-loaded niosomes against MRSA strains. In this study, six carvacrol–niosome formulations with different ratios of Span and Tween were prepared. The physicochemical attributes of the optimized synthesized niosomes were assessed using Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) as well as DLS Zetasizer. Encapsulation efficiency (EE) and <em>in vitro</em> drug release were studied. The antibacterial activity of the synthesized carvacrol–niosomes, in concentrations varying between 7.8 and 1000<!--> <!-->μg/ml, was evaluated using microdilution broth methods. The optimized niosomes, with a size of 207.3<!--> <!-->nm and an impressive EE of 91%, exhibited a spherical structure as confirmed by the electron microscopy analysis. Impressively, these carvacrol–niosomes demonstrated superior antimicrobial effectiveness against <em>S. aureus</em>, reducing MIC levels 4-fold to 62.5<!--> <!-->±<!--> <!-->0.0<!--> <!-->μg/ml and MBC to 125<!--> <!-->±<!--> <!-->0.0<!--> <!-->μg/ml, a significant improvement over the 250<!--> <!-->±<!--> <!-->0.0<!--> <!-->μg/ml MIC and 500<!--> <!-->±<!--> <!-->0.0<!--> <!-->μg/ml MBC of free carvacrol. Additionally, while empty niosomes showed minimal cytotoxicity with 88.32<!--> <!-->±<!--> <!-->1.32% cell viability at 100<!--> <!-->μg/ml, free carvacrol led to a marked reduction in viability to 39.46<!--> <!-->±<!--> <!-->1.26%. However, niosomes encapsulating carvacrol notably increased cell survival to 59.67<!--> <!-->±<!--> <!-->1.62% at this concentration. These findings underscore the enhanced antimicrobial potency of carvacrol when enclosed within niosomes, suggesting its potential as a potent herbal remedy for combating methicillin-resistant <em>S. aureus</em>.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 39-48"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staphylococcus aureus resistentes a meticilina aislados de teléfonos móviles de estudiantes de Enfermería en Cuenca, Ecuador","authors":"Nube María Tenezaca Lliguin ,&nbsp;Paola Patricia Orellana Bravo ,&nbsp;Carlos Fernando Andrade Tacuri ,&nbsp;Jonnathan Gerardo Ortiz Tejedor","doi":"10.1016/j.ram.2024.12.002","DOIUrl":"10.1016/j.ram.2024.12.002","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> is an important pathogen in healthcare facilities, with its resistance to a number of antibiotics currently being a global concern. In this report the presence of <em>S.<!--> <!-->aureus</em>, resistance gene virulence and antibiotic susceptibility profiles were determined in the mobile phones of senior nursing students. <em>S.<!--> <!-->aureus</em> was isolated in 11.84% (9/76) of the samples. Furthermore, 44.44% of the mobile phones carried the <em>mecA</em> (MRSA) gene, while none carried the <em>vanA</em> gene. Virulence genes identified were 100% <em>hla</em>, 88.89% <em>hlb</em>, 22.22% <em>tst</em> and <em>sec</em>, and 11.11% <em>sea</em>. The antibiogram revealed that 33.33% of the strains were resistant to cefoxitin and 44.44% showed inducible resistance to clindamycin (ICRSA). The mobile phones of senior nursing students represent an important reservoir of drug-resistant and virulent strains of <em>S.<!--> <!-->aureus</em>, which could act as infectious foci for the transmission of this pathogen.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 54-58"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symbiotic nitrogen-fixing rhizobia as a potential source of nitrous oxide emissions","authors":"Aníbal R. Lodeiro","doi":"10.1016/j.ram.2025.01.003","DOIUrl":"10.1016/j.ram.2025.01.003","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 1-2"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}