Revista Argentina de microbiologia最新文献

{"title":"Composition in phyla from breast milk: Effect of the mode of delivery.","authors":"Shiva Aslani, Seyedeh Neda Mousavi, Atefeh Kazemi Robati, Siamak Heidarzadeh, Somaye Abdollahi Sabet","doi":"10.1016/j.ram.2025.04.001","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.001","url":null,"abstract":"<p><p>Breast milk is considered as a living ecosystem. Maternal, environmental and neonatal factors affect milk bacterial composition. The aim of present study was to assess the phyla from breast milk of mothers with vaginal delivery compared to the cesarean section. In this single-center case-control study, sixty women were participated. Half of them had vaginal delivery and others experienced cesarean section. The breast milk samples were collected three months after delivery for the DNA extraction to measure Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria using quantitative real time chain polymerase reaction. Daily intake of calories, protein, fat, carbohydrate and fiber did not differ significantly between the two groups. The proportion of Bacteroidetes, Firmicutes and Proteobacteria were significantly higher in milk of mothers with a cesarean section than the vaginal delivery (p=0.03, p=0.02 and p=0.042). Similarly, the Firmicutes to Bacteroides ratio was significantly increased (p=0.02). The Actinobacteria population was significantly higher in milk of vaginally-delivered mothers who had male infant than females (p=0.015). Breast milk of mothers with cesarean section showed alterations in the main bacterial phyla population compared to the vaginal delivery. Moreover, our results suggest that the sex of infant is an effective factor on some phyla quantity.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity assessment of Bacillus thuringiensis strains for the control of the lesser mealworm beetle Alphitobius diaperinus (Coleoptera: Tenebrionidae).","authors":"Melisa P Pérez, Graciela B Benintende, Diego H Sauka","doi":"10.1016/j.ram.2025.03.003","DOIUrl":"https://doi.org/10.1016/j.ram.2025.03.003","url":null,"abstract":"<p><p>This study addresses the pervasive challenge of lesser mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae) infestations in poultry farming. Our aim was to select toxic Bacillus thuringiensis strains against A. diaperinus larvae and determine the fraction(s) within the bacterial cultures harboring the active metabolites responsible for this insecticidal activity. Among the 41 strains evaluated, the Argentine strain INTA Mo4-4 showed the highest toxicity. Bioassays revealed that the main virulence factors reside within the spore-crystal pellet associated with the Cry protein. These results set the basis for the development of a targeted bioinsecticide. Further research is needed to elucidate the underlying mechanisms and validate these strategies in field trials. By harnessing B. thuringiensis-based bioinsecticides, we offer a sustainable solution to mitigate A. diaperinus infestations.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of intestinal microbiota diversity in children with non-organic anorexia.","authors":"Wei Lu, Zong-Long Li, De-Yong Xu, Guo-Ping Yu","doi":"10.1016/j.ram.2025.02.001","DOIUrl":"https://doi.org/10.1016/j.ram.2025.02.001","url":null,"abstract":"<p><p>The pathogenesis of non-organic anorexia in children is not clear. This study intends to analyze intestinal bacteria to provide a relevant theoretical basis for the clinical rational selection of microecological agents. In the present study, children with non-organic anorexia were included in the anorexia group and normal healthy children in the control group. Stool samples were collected for the bioinformatics analysis after PCR and high-throughput sequencing. The results showed that the Ace, Chao, and Shannon indexes in the anorexia group were higher than those in the control group, while the Simpson index in the control group was lower than in the anorexia group. There were 14 taxa in the anorexia group and 11 taxa in the healthy control group at the phylum level, and 193 taxa in the anorexia group and 180 in the control group at the genus level. The dominant bacteria at the phylum level of the two groups were the same, while there were 16 dominant bacteria taxa in the anorexia group and 17 in the control group at the genus level. The ratio of percentage abundance of Bacteroidetes to that of Firmicutes (the B/F index) in the anorexia group was higher than in the control group. The abundance of Bacteroidetes in the anorexia group was higher than that in the control group, and the abundance of Actinomycetes in the control group was higher than that in the anorexia group. There were significant differences in 14 dominant genera between the two groups at the genus classification level. The LEfSe multilevel species difference analysis showed that at the phylum level, the significant influential bacterial taxa in the anorexia group were Bacteroidetes and Actinobacteria in the control group. At the genus level, the significant influential bacterial taxa in the anorexia group were Bacteroides, Faecalibacterium, and Subdoligranulum, and Bifidobacterium, Blautia, Streptococcus, Lachnoclostridium, and Erysipelatoclostridium in the control group. We conclude that the increase in Bacteroides abundance or in the B/F index and the reduction in Bifidobacterium abundance were related to the pathogenesis of anorexia.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospective evaluation of Leptospira spp. infection in goats maintained in field conditions in the Caatinga biome, Brazil.","authors":"Mayla de Lisbôa Padilha, Lídio Ricardo Bezerra de Melo, Clécio Henrique Limeira, Nathália Maria de Andrade Magalhães, Maria Luana Cristiny Rodrigues Silva, Rafael Rodrigues Soares, Clebert José Alves, Severino Silvano Dos Santos Higino, Carolina de Sousa Américo Batista Santos, Sérgio Santos de Azevedo","doi":"10.1016/j.ram.2025.03.001","DOIUrl":"https://doi.org/10.1016/j.ram.2025.03.001","url":null,"abstract":"<p><p>The Caatinga biome occurs only in Brazil; however, there is no survey on leptospirosis using direct and indirect diagnostic tests in this region with goats maintained in field conditions. We conducted a prospective survey with paired sampling to evaluate the importance of carrier goats in the maintenance of disease. Based on sampling calculation, 60 goats (30 confined and 30 extensively reared) were randomly selected and monitored for three months during the rainy season with paired monthly biological sample collections. The animals underwent urine and vaginal fluid collection for microbiological and molecular diagnosis, and serum samples for serological diagnosis using the microscopic agglutination test (MAT). Overall, 45 (75%) animals were positive in at least one diagnostic method. Anti-Leptospira spp. antibodies were detected in all three sampling moments in 43 (71.7%) animals, antibody titers varied from 25 to 800, and most frequent serogroups were Australis in the 1st and 2nd blood collections (66.7% and 40.7%, respectively) and Cynopteri in the 3rd collection (52.6%). None of the animals tested positive in the microbiological diagnosis or the vaginal fluid PCR; however, five (16.7%) animals were positive in the urine PCR only in the confined group in the first collection. Two DNA urine samples were sequenced, demonstrating 99% similarity with Leptospira interrogans. Different diagnostic techniques for leptospirosis in goats raised under Caatinga biome field conditions are suggested, as well as further studies over a longer period with monthly collections to better understand the prevalence of Leptospira spp. and its variation over time.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall structure and its influence on aflatoxin B1 binding","authors":"Carina Pereyra ,&nbsp;María del Pilar Monge ,&nbsp;Silvestre Bongiovanni ,&nbsp;Andrea Cristofolini ,&nbsp;Sergio Campos ,&nbsp;Lilia Cavaglieri","doi":"10.1016/j.ram.2024.07.004","DOIUrl":"10.1016/j.ram.2024.07.004","url":null,"abstract":"<div><div>The aim of this study was to determine the impact of <em>Kluyveromyces marxianus</em> VM004 culture conditions on the cell wall (CW) structure and its influence on aflatoxin B₁ binding. The yeast was inoculated into two types of culture media: yeast extract-peptone-dextrose (YPD) broth and dried distiller's grains with solubles (DDG). The CW was extracted from the biomass produced in these media. AFB₁ (150<!--> <!-->ng/ml) adsorption tests using the biomass (1<!--> <!-->×<!--> <!-->10⁷ <!-->cells/ml) and the CW (0.001<!--> <!-->g) were performed at pH 2 and pH 8. Transmission electron microscopy (TEM) evaluated the CW thickness, and infrared spectroscopy (IR) determined the CW composition. Biomass production in YPD was higher than that in DDG. Cell diameter (μm) and CW thickness (μm) increased in the DDG medium. The CW percentage obtained in DDG was higher than that in YPD. The absorbance of carbohydrates by IR was higher in YPD. pH influenced AFB₁ adsorption, which was lower at pH 8. The proportion of β-glucan and chitin present in CW was higher in the YPD medium. The IR method allowed to study the CW carbohydrate variation under the influence of these carbon sources. In conclusion, the culture media composition influenced the β-glucan and chitin composition and consequently, AFB₁ adsorption.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 89-97"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infecciones relacionadas con la asistencia sanitaria en una unidad de cuidados intensivos pediátricos en México: epidemiología y factores asociados","authors":"Juan Carlos Lona-Reyes ,&nbsp;Tania Alejandra Cruz-Chávez ,&nbsp;Juan Antonio Gallegos-Marín ,&nbsp;Ana María Chávez-Vázquez ,&nbsp;Fernando Alatorre-Rendón ,&nbsp;Jesús González-Carmona ,&nbsp;Bruno Moreno-Medina","doi":"10.1016/j.ram.2024.12.012","DOIUrl":"10.1016/j.ram.2024.12.012","url":null,"abstract":"<div><div>We conducted a prolective cohort study with the aim of estimating the incidence of healthcare-associated infections and identifying associated factors in an intensive care unit in Mexico. Diagnosis of central venous catheter-associated bacteremia, ventilator-associated pneumonia and urinary catheter-associated urinary tract infection was established according to the Centers for Disease Control and Prevention definitions; risk factors were analyzed by logistic regression. Four hundred twenty-six patients who had 486 admissions were studied, 55.9% were male and the median age was 4 years. The healthcare-associated infections incidence rate was 14.8 events/1000 patient-days. The prevalent microorganisms were gram negative bacilli. The factors associated with healthcare-associated infections were chronic conditions (<em>p</em> <!-->=<!--> <!-->0,01), the number of central venous catheters inserted and the days duration of central venous catheter, mechanical ventilation and the urinary catheter (the 4 variables with <em>p</em> <!-->≤<!--> <!-->0.001).</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 136-141"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial richness assessment in water and sediments in the northern coast of the Yucatan Peninsula","authors":"Mariela Beatriz Reyes-Sosa ,&nbsp;Raúl Valle-Gough ,&nbsp;María del Carmen Ponce-Caballero ,&nbsp;María Leticia Arena-Ortiz","doi":"10.1016/j.ram.2024.10.009","DOIUrl":"10.1016/j.ram.2024.10.009","url":null,"abstract":"<div><div>Given the importance of the coastal environments and the multiple ecological services that they provide, it is important to explore and understand the interactions that occur within them. The microbiome is a key factor for the understanding of the dynamics of these fragile sites. A metagenomic study based on the profiling of the 16S ribosomal gene was carried out in order to assess the bacterial diversity present in the northern coastal zone of the Yucatan Peninsula. The results showed that water and sediment samples share some similarities regarding the bacterial genera found, only differing in the quantitative part. Through a PCO (principal coordinates) analysis clear differences between sediment and water samples could be observed. The highest relative diversity was found in wetland and lagoon sediment samples, respectively. It was observed that 3–8% of the total sequence reads belonged to opportunistic genera such as: <em>Vibrio</em> in the sea samples and <em>Capnocytophaga</em> in the other environments. Salinity and pH were the factors which contributed the most to the differences among the communities in the various environments in the coastal zone. There is an important similarity in the sediments across the different environments within the studied coastal zone. The data presented herein contribute to setting a baseline for research in the coastal region of the Yucatan Peninsula.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 171-181"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Broaden properties of ambroxol hydrochloride as an antibiofilm compound","authors":"María J. González ,&nbsp;Michela Lain ,&nbsp;Victoria Iribarnegaray ,&nbsp;Luciana Robino ,&nbsp;Paola Scavone","doi":"10.1016/j.ram.2024.10.010","DOIUrl":"10.1016/j.ram.2024.10.010","url":null,"abstract":"<div><div>Biofilm-associated microorganisms can cause many infections and are an important cause of resistance to several antimicrobials. The antibiotic crisis has led to a pressing need for new therapeutic tools. Ambroxol is frequently used as a mucolytic agent in respiratory diseases with increased mucus production. In addition, a wide range of properties has been described, including the effect on biofilms. In this work, we evaluate the anti-biofilm effect of ambroxol on four strains with clinical relevance: <em>Proteus mirabilis</em>, <em>Escherichia coli</em>, <em>Staphylococcus aureus</em>, and <em>Acinetobacter baumannii</em>. In vitro, biofilm formation was assessed using the crystal violet quantification technique in microplate and glass coverslip. The inhibition of biofilm formation was evaluated by adding ambroxol at the initial time. Ambroxol hydrochloride was evaluated over the preformed biofilm and live/dead bacteria were quantified. The effect of ambroxol in the ethidium bromide efflux assay and the relative expression of the five major <em>P. mirabilis</em> efflux pump family genes were analyzed. Ambroxol inhibited biofilm formation in all the bacteria tested. Moreover, ambroxol significantly reduces both biofilm biomass and viable bacteria. Ambroxol was able to affect <em>P. mirabilis</em> efflux pumps depending on the concentration used and induced the overexpression of several efflux pump genes. In summary, ambroxol kills planktonic cells, reduce biofilm biomass as it increases cell death, and affect the expression of efflux pumps. Furthermore, it presents a viable alternative for the treatment of biofilm infection alone or in combination with antibiotic therapy.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 114-124"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo study of the antifungal activity of extracellular products of cyanobacterium Neowestiellopsis persica strain A1387 against Fusarium wilt disease of cucumber","authors":"Bahareh Nowruzi,&nbsp;Hassan Beiranvand","doi":"10.1016/j.ram.2024.10.011","DOIUrl":"10.1016/j.ram.2024.10.011","url":null,"abstract":"<div><div><em>Fusarium</em> wilt of cucumber, caused by the fungus <em>Fusarium oxysporum</em>, is a major plant disease that causes significant economic losses. The extensive use of chemical fungicides for its control poses environmental and health risks. Due to growing concerns about the detrimental effects of chemical fungicides, finding safe and effective bio-based alternatives for plant disease control is of high importance. In this study, the potential of <em>Neowestiellopsis persica</em> A1387 cyanobacterial metabolites as a promising substitute for chemical fungicides in controlling this disease was investigated. The antifungal activity of <em>N. persica</em> A1387 cyanobacterial exopolysaccharide (EPS) extract was evaluated against <em>F. oxysporum</em> under <em>in vitro</em> and <em>in vivo</em> conditions. Cucumber plants infected with the fungus were treated with cyanobacterial EPS extract and then assessed for disease severity, antioxidant enzyme activity, and growth parameters. Both biomass and EPS extracts of <em>N. persica</em> A1387 cyanobacteria significantly increased the diameter of the <em>F. oxysporum</em> growth inhibition zone under <em>in vitro</em> conditions. Treatment with cyanobacterial EPS extract resulted in increased dry and fresh weight of stem and roots, and a significant reduction in disease severity and percentage in <em>F. oxysporum</em>-infected plants. Peroxidase, superoxide dismutase (SOD), and catalase enzyme activities in fungus-infected plants treated with cyanobacterial EPS extract were significantly lower on day 42 of infection compared to untreated and infected control plants. These findings demonstrate the potential of <em>N. persica</em> A1387 cyanobacterial extracts as natural and safe alternatives to chemical fungicides for controlling cucumber <em>Fusarium</em> wilt disease.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 182-191"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal and antioxidant potential of Ocimum species against Ascochyta rabiei (Pass) Lab","authors":"Tooba Shafiq ,&nbsp;Khajista Jabeen ,&nbsp;Sumera Iqbal ,&nbsp;Aisha Umar ,&nbsp;Mohamed S. Elshikh ,&nbsp;Reem M. Aljowaie ,&nbsp;Laurent Dufossé ,&nbsp;Rashid Iqbal ,&nbsp;Soumya Ghosh","doi":"10.1016/j.ram.2025.02.003","DOIUrl":"10.1016/j.ram.2025.02.003","url":null,"abstract":"<div><div><em>Cicer arietinum</em> L. is a vital source of nutrients that suffers substantial annual losses due to Ascochyta blight, caused by the plant pathogen <em>Ascochyta rabiei</em>. This study aimed to investigate the antifungal potential of <em>Ocimum tenuiflorum</em> L. and <em>O. basilicum</em> L. shoots (leaves and stems) against <em>A. rabiei</em> (Pass) Lab. <em>In vitro</em> bioassays were conducted using methanolic extracts from leaves and stems at six different concentrations: 1%, 1.5%, 2%, 2.5%, 3%, and 3.5%. A total of eight compounds were identified through gas chromatography–mass spectrometry (GC–MS) analysis. The highest inhibition of <em>A. rabiei</em> growth was achieved with a 3.5% methanolic leaf extract of <em>O. basilicum</em>. Methanolic extracts from <em>O. tenuiflorum</em> shoots also reduced fungal growth by 6.18–73%. Additionally, the n-hexane fraction derived from <em>O. basilicum</em> inhibited fungal growth by 71–76% and was subsequently analyzed using GC–MS. This analysis identified eight compounds: (1) cyclopentane, methyl-, (2) cyclohexane, (3) 2,2-dimethylbutane, (4) 2,3-dimethylbutane, pentane, (5) 2,3-dimethyl-, (6) 2-bromoacetonitrile, (7) alpha-cadinol, and (8) phenylpropanolamine. The antioxidant activity of <em>O. tenuiflorum</em> and <em>O. basilicum</em> shoots was also assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The highest antioxidant activity, 98.58%, was recorded at a 3.5% methanolic stem extract concentration of <em>O. tenuiflorum</em>. The antioxidant activity potential was highest for <em>O. tenuiflorum</em> at 0.729<!--> <!-->mg/mL, followed by <em>O. basilicum</em> at 0.411<!--> <!-->mg/mL.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 125-135"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}