Revista Argentina de microbiologia最新文献

Impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall structure and its influence on aflatoxin B₁ binding. Kluyveromyces marxianus VM004 培养条件对细胞壁结构的影响及其对黄曲霉毒素 B1 结合力的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-09-20 DOI: 10.1016/j.ram.2024.07.004

Carina Pereyra, María Del Pilar Monge, Silvestre Bongiovanni, Andrea Cristofolini, Sergio Campos, Lilia Cavaglieri

{"title":"Impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall structure and its influence on aflatoxin B1 binding.","authors":"Carina Pereyra, María Del Pilar Monge, Silvestre Bongiovanni, Andrea Cristofolini, Sergio Campos, Lilia Cavaglieri","doi":"10.1016/j.ram.2024.07.004","DOIUrl":"https://doi.org/10.1016/j.ram.2024.07.004","url":null,"abstract":"The aim of this study was to determine the impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall (CW) structure and its influence on aflatoxin B1 binding. The yeast was inoculated into two types of culture media: yeast extract-peptone-dextrose (YPD) broth and dried distiller's grains with solubles (DDG). The CW was extracted from the biomass produced in these media. AFB1 (150ng/ml) adsorption tests using the biomass (1×107cells/ml) and the CW (0.001g) were performed at pH 2 and pH 8. Transmission electron microscopy (TEM) evaluated the CW thickness, and infrared spectroscopy (IR) determined the CW composition. Biomass production in YPD was higher than that in DDG. Cell diameter (μm) and CW thickness (μm) increased in the DDG medium. The CW percentage obtained in DDG was higher than that in YPD. The absorbance of carbohydrates by IR was higher in YPD. pH influenced AFB1 adsorption, which was lower at pH 8. The proportion of β-glucan and chitin present in CW was higher in the YPD medium. The IR method allowed to study the CW carbohydrate variation under the influence of these carbon sources. In conclusion, the culture media composition influenced the β-glucan and chitin composition and consequently, AFB1 adsorption.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptococcus spp. isolated from bovine mastitis: Antimicrobial sensitivity studies and disagreement evaluation between routine phenotypic diagnosis and molecular identification. 从牛乳腺炎中分离出的链球菌：抗菌药敏感性研究以及常规表型诊断与分子鉴定之间的分歧评估。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-09-19 DOI: 10.1016/j.ram.2024.07.006

Leticia Diana, Lucas Mastroianni, Virginia Diana, Rodrigo Puentes

{"title":"Streptococcus spp. isolated from bovine mastitis: Antimicrobial sensitivity studies and disagreement evaluation between routine phenotypic diagnosis and molecular identification.","authors":"Leticia Diana, Lucas Mastroianni, Virginia Diana, Rodrigo Puentes","doi":"10.1016/j.ram.2024.07.006","DOIUrl":"https://doi.org/10.1016/j.ram.2024.07.006","url":null,"abstract":"Bovine mastitis poses a significant threat to global dairy production, resulting in substantial losses in milk production. Streptococcus bacteria, particularly Streptococcus uberis, Streptococcus agalactiae, and Streptococcus dysgalactiae, are commonly implicated in this condition. An accurate diagnosis is crucial for implementing effective treatment and minimizing its impact on production. This study examined 115 Streptococcus strains isolated from bovine mastitis cases in Uruguay using PCR for species identification. Additionally, the resistance to tetracycline, erythromycin, and penicillin was assessed in 81 of the bacterial strains under study. Significant disparities between phenotypic and genotypic detection were evident across all three species, with only 31% of strains identified phenotypically aligning with PCR results. Phenotypic prevalence indicated S. dysgalactiae as the most prevalent (44.35%), followed by S. uberis (24.34%) and S. agalactiae (6.09%). However, the genotypic identification revealed S. uberis as the most prevalent, followed by S. dysgalactiae, while S. agalactiae remained the least prevalent. The high sensitivity and speed of PCR suggest its potential routine implementation for diagnosing bovine mastitis caused by Streptococcus in any laboratory. Although, penicillin resistance was practically nonexistent, tetracycline and erythromycin exhibit higher resistance levels across all three species studied. In conclusion, the study underlines the importance of early diagnosis, highlights variations in bacterial prevalence, and proposes PCR as a valuable diagnostic tool for Streptococcus species responsible for bovine mastitis.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaerobic digestates in agricultural soils: A systematic review of their effects on antibiotic resistance genes. 农业土壤中的厌氧消化物：系统回顾其对抗生素抗性基因的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-09-18 DOI: 10.1016/j.ram.2024.07.005

Marco Allegrini, María Celina Zabaloy

{"title":"Anaerobic digestates in agricultural soils: A systematic review of their effects on antibiotic resistance genes.","authors":"Marco Allegrini, María Celina Zabaloy","doi":"10.1016/j.ram.2024.07.005","DOIUrl":"https://doi.org/10.1016/j.ram.2024.07.005","url":null,"abstract":"Tackling the dissemination of antibiotic resistance is one of the main global challenges. Manures from animal production are a recognized source of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) requiring appropriate treatment methods. One of the main approaches for manure treatment is anaerobic digestion (AD). Meta-analyses have demonstrated that AD can significantly reduce the load of ARGs. However, antibiotics, ARGs and MGEs still remain in the final product (digestate). A sustainable agricultural use of digestates under the One Health framework requires wide assessments of their effects in the soil resistome. The objective of this review was to present the state of the art of digestate effects on ARGs of agricultural soils, focusing exclusively on digestates from animal manures. A systematic review was conducted. The examination of the resulting literature indicated that although temporal decays are observed for a variety of ARGs in single-application and repeated-applications experiments, for certain ARGs the pre-treatment or control levels are not restored. However, the low number of studies and the heterogeneous experimental conditions preclude a clear understanding of the fate of ARGs in soil and their risk for agroecosystems. The inclusion of multiple MGEs and the assessment of the long-term influence of digestates on soil properties and microbial communities could be keystones for a better understanding of the risks associated with digestate-induced changes in the soil resistome.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of EPEC and STEC strains isolated from children with diarrhea in Argentina. 检测从阿根廷腹泻儿童中分离出的 EPEC 和 STEC 菌株。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-09-13 DOI: 10.1016/j.ram.2024.07.003

Daniel Fernández Fellenz, Julia M Ruiz, Analía I Etcheverría, Rocio Colello, María V Velez, Marcelo E Sanz, Mónica D Sparo, Sabina Lissarrague, Josefina Pereyra, Gustavo Zanelli, Nora L Padola

引用次数: 0

[Educational intervention as a prevention strategy for intestinal parasitosis, and assessment of the enteroparasitological status in child development centers in the city of Santa Fe, Argentina]. [将教育干预作为预防肠道寄生虫病的策略，并评估阿根廷圣菲市儿童发展中心的肠道寄生虫病状况]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-09-02 DOI: 10.1016/j.ram.2024.07.002

César Ernesto Gutiérrez, María Agustina Jaras Horno, Carolina Aro, Andrea Florencia Gómez Colussi, María Belén Cheirano, Érica Soledad Rodríguez, María Verónica Vera Garate, Guillermo García Effron, Sergio Adrián Guerrero

{"title":"[Educational intervention as a prevention strategy for intestinal parasitosis, and assessment of the enteroparasitological status in child development centers in the city of Santa Fe, Argentina].","authors":"César Ernesto Gutiérrez, María Agustina Jaras Horno, Carolina Aro, Andrea Florencia Gómez Colussi, María Belén Cheirano, Érica Soledad Rodríguez, María Verónica Vera Garate, Guillermo García Effron, Sergio Adrián Guerrero","doi":"10.1016/j.ram.2024.07.002","DOIUrl":"https://doi.org/10.1016/j.ram.2024.07.002","url":null,"abstract":"Intestinal parasitic diseases constitute a cosmopolitan public health concern, with greater prevalence in developing countries, and mainly affecting children. The aim of this study was to develop an educational intervention aimed at mothers/guardians of children attending three child development centers (CDI) in Santa Fe, Argentina, during 2018. An educational intervention was conducted using a descriptive, quasi-experimental design, with pre- and post-intervention assessment. This intervention included 36 mothers, and was carried out in three stages: diagnosis, intervention and final evaluation. Simultaneously, a coproparasitological study was performed on 48 children under 5 years of age, who were assisted in the CDI, with the aim to understand and address the parasitic infections prevalent in the child population. It was possible to compare the correct answers before and after the educational intervention, observing a statistically significant increase (p=0.008742) in the average number of correct answers. Enteroparasites were identified in 54% of the population of children analyzed, 10% were biparasitized and 6% had more than two parasitic species. The most frequently detected parasites were Blastocystis spp., Giardia intestinalis and Ascaris lumbricoides. The educational intervention was positive, resulting in an improvement in the level of knowledge related to intestinal parasitosis and its prevention. This educational intervention experience highlights the importance of ongoing education in promoting a healthy lifestyle and preventing parasitosis in vulnerable populations.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Norovirus outbreaks in a nursery school in Buenos Aires, Argentina. 阿根廷布宜诺斯艾利斯一家幼儿园爆发诺如病毒。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-09-02 DOI: 10.1016/j.ram.2024.07.001

Karina A Gomes, Juan I Degiuseppe, Juan A Stupka

引用次数: 0

Low-cost antiseptic-impregnated tracheostomy tube for the prevention of ventilator-associated pneumonia caused by multidrug-resistant bacteria: In vitro and pilot study in humans. 用于预防耐多药细菌引起的呼吸机相关肺炎的低成本消毒浸渍气管造口管：体外和人体试验研究。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-07-26 DOI: 10.1016/j.ram.2024.05.004

Juliano Gasparetto, Leandro Bressianini Jurkonis, Leticia Ramos Dantas, Paula Hansen Suss, Felipe Francisco Tuon

引用次数: 0

Prokaryotes versus Eukaryotes: Which is the host and which is the guest? 原核生物与真核生物：哪个是主人，哪个是客人？

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-07-01 DOI: 10.1016/j.ram.2024.09.001

引用次数: 0

Caracterización de una comunidad microbiana aislada de colonias de abejas melíferas [从蜜蜂群中分离出来的微生物群落的特征]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-07-01 DOI: 10.1016/j.ram.2024.01.001

{"title":"Caracterización de una comunidad microbiana aislada de colonias de abejas melíferas","authors":"","doi":"10.1016/j.ram.2024.01.001","DOIUrl":"10.1016/j.ram.2024.01.001","url":null,"abstract":"<div>The microbial communities within honey bee colonies contribute to the defense against pathogens. The goal of this study was to isolate, identify, and lyophilize lactic acid bacteria and bifidobacteria from the gut of nurse bees and bee bread in Apis mellifera colonies. Bacterial cultures from the intestinal content were conducted, and subsequently identified, sequenced, and lyophilized. Cross-antagonism among them was also assessed. Studies based on 16 S rRNA gene Sanger sequencing revealed that the MC3 strain had 100% identity with Bifidobacterium choladohabitans, the PP2B strain showed 99.16% similarity with Enterococcus faecium, while the PP1 strain exhibited 99.49% similarity with Lacticaseibacillus sp. and the PP1B strain showed 99.32% similarity with Lacticaseibacillus sp. There was no evidence of cross-antagonism among the strains, and the lyophilization process showed good stability and conservation. This is the first report of the isolation of B. choladohabitans from honey bee gut in Argentina, and also associates the presence of E. faecium with bee bread.</div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000245/pdfft?md5=6493003c0502128f9f6bb57f28eb42bb&pid=1-s2.0-S0325754124000245-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140958925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obtaining more contaminant-resistant variants from a native Chlorella vulgaris strain 从本地绿藻菌株中获取更多抗污染变种。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-07-01 DOI: 10.1016/j.ram.2024.05.005

{"title":"Obtaining more contaminant-resistant variants from a native Chlorella vulgaris strain","authors":"","doi":"10.1016/j.ram.2024.05.005","DOIUrl":"10.1016/j.ram.2024.05.005","url":null,"abstract":"<div>Cildáñez stream (in Matanza-Riachuelo basin, Buenos Aires) is one of the most polluted watercourses of Argentina, containing a mixed contamination from agricultural and industrial wastes. The application of water bioremediation processes for this kind of effluent will require microorganisms with a high tolerance to contamination. In this sense, obtaining higher contaminant-resistant microalgae lines is widely desired. In this study, adaptive laboratory evolution (ALE) and random mutagenesis were used to obtain Chlorella vulgaris LMPA-40 strains adapted to grow in polluted water from the Cildáñez stream. The ALE process was performed by 22 successive subcultures under selective pressure (Cildáñez wastewater alone or with the addition of phenol or H2O2) while random mutagenesis was performed with UV-C radiation at 275nm. Not all the cell lines obtained after ALE could adapt enough to overcome the stress caused by the Cildáñez wastewater, indicating that the process is quite random and depends on the stressor used. The best results were obtained for the Cildáñez wastewater adapted cells (Cild 3 strain) that were more resistant than the original strain. The concentration of protein, Chlorophyll A, Chlorophyll B, and carotenoids in the Cild 3 ALE evolved strain was higher than that of the control strain. However, this strain exhibited half of the lipid content compared to the same control strain. Interestingly, these alterations and the acquired tolerance may be reversed over time during storage. These findings suggest that the acquisition of novel cell lines could not be permanent, a fact that must be considered for future trials.</div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000816/pdfft?md5=7d5205e469aa55049c1fcd77cc9f95be&pid=1-s2.0-S0325754124000816-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0