Revista Argentina de microbiologia最新文献

Prospective evaluation of Leptospira spp. infection in goats maintained in field conditions in the Caatinga biome, Brazil. 巴西Caatinga生物群系野外条件下山羊钩端螺旋体感染的前瞻性评价。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-07 DOI: 10.1016/j.ram.2025.03.001

Mayla de Lisbôa Padilha, Lídio Ricardo Bezerra de Melo, Clécio Henrique Limeira, Nathália Maria de Andrade Magalhães, Maria Luana Cristiny Rodrigues Silva, Rafael Rodrigues Soares, Clebert José Alves, Severino Silvano Dos Santos Higino, Carolina de Sousa Américo Batista Santos, Sérgio Santos de Azevedo

{"title":"Prospective evaluation of Leptospira spp. infection in goats maintained in field conditions in the Caatinga biome, Brazil.","authors":"Mayla de Lisbôa Padilha, Lídio Ricardo Bezerra de Melo, Clécio Henrique Limeira, Nathália Maria de Andrade Magalhães, Maria Luana Cristiny Rodrigues Silva, Rafael Rodrigues Soares, Clebert José Alves, Severino Silvano Dos Santos Higino, Carolina de Sousa Américo Batista Santos, Sérgio Santos de Azevedo","doi":"10.1016/j.ram.2025.03.001","DOIUrl":"https://doi.org/10.1016/j.ram.2025.03.001","url":null,"abstract":"<p><p>The Caatinga biome occurs only in Brazil; however, there is no survey on leptospirosis using direct and indirect diagnostic tests in this region with goats maintained in field conditions. We conducted a prospective survey with paired sampling to evaluate the importance of carrier goats in the maintenance of disease. Based on sampling calculation, 60 goats (30 confined and 30 extensively reared) were randomly selected and monitored for three months during the rainy season with paired monthly biological sample collections. The animals underwent urine and vaginal fluid collection for microbiological and molecular diagnosis, and serum samples for serological diagnosis using the microscopic agglutination test (MAT). Overall, 45 (75%) animals were positive in at least one diagnostic method. Anti-Leptospira spp. antibodies were detected in all three sampling moments in 43 (71.7%) animals, antibody titers varied from 25 to 800, and most frequent serogroups were Australis in the 1st and 2nd blood collections (66.7% and 40.7%, respectively) and Cynopteri in the 3rd collection (52.6%). None of the animals tested positive in the microbiological diagnosis or the vaginal fluid PCR; however, five (16.7%) animals were positive in the urine PCR only in the confined group in the first collection. Two DNA urine samples were sequenced, demonstrating 99% similarity with Leptospira interrogans. Different diagnostic techniques for leptospirosis in goats raised under Caatinga biome field conditions are suggested, as well as further studies over a longer period with monthly collections to better understand the prevalence of Leptospira spp. and its variation over time.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall structure and its influence on aflatoxin B1 binding Kluyveromyces marxianus VM004 培养条件对细胞壁结构的影响及其对黄曲霉毒素 B1 结合力的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.07.004

Carina Pereyra , María del Pilar Monge , Silvestre Bongiovanni , Andrea Cristofolini , Sergio Campos , Lilia Cavaglieri

{"title":"Impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall structure and its influence on aflatoxin B1 binding","authors":"Carina Pereyra , María del Pilar Monge , Silvestre Bongiovanni , Andrea Cristofolini , Sergio Campos , Lilia Cavaglieri","doi":"10.1016/j.ram.2024.07.004","DOIUrl":"10.1016/j.ram.2024.07.004","url":null,"abstract":"<div><div>The aim of this study was to determine the impact of <em>Kluyveromyces marxianus</em> VM004 culture conditions on the cell wall (CW) structure and its influence on aflatoxin B<sub>1</sub> binding. The yeast was inoculated into two types of culture media: yeast extract-peptone-dextrose (YPD) broth and dried distiller's grains with solubles (DDG). The CW was extracted from the biomass produced in these media. AFB<sub>1</sub> (150ng/ml) adsorption tests using the biomass (1×10<sup>7</sup>  <!-->g) were performed at pH 2 and pH 8. Transmission electron microscopy (TEM) evaluated the CW thickness, and infrared spectroscopy (IR) determined the CW composition. Biomass production in YPD was higher than that in DDG. Cell diameter (μm) and CW thickness (μm) increased in the DDG medium. The CW percentage obtained in DDG was higher than that in YPD. The absorbance of carbohydrates by IR was higher in YPD. pH influenced AFB<sub>1</sub> adsorption, which was lower at pH 8. The proportion of β-glucan and chitin present in CW was higher in the YPD medium. The IR method allowed to study the CW carbohydrate variation under the influence of these carbon sources. In conclusion, the culture media composition influenced the β-glucan and chitin composition and consequently, AFB<sub>1</sub> adsorption.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 89-97"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infecciones relacionadas con la asistencia sanitaria en una unidad de cuidados intensivos pediátricos en México: epidemiología y factores asociados [墨西哥儿童重症监护病房的医疗保健相关感染：流行病学和相关因素]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.12.012

Juan Carlos Lona-Reyes , Tania Alejandra Cruz-Chávez , Juan Antonio Gallegos-Marín , Ana María Chávez-Vázquez , Fernando Alatorre-Rendón , Jesús González-Carmona , Bruno Moreno-Medina

{"title":"Infecciones relacionadas con la asistencia sanitaria en una unidad de cuidados intensivos pediátricos en México: epidemiología y factores asociados","authors":"Juan Carlos Lona-Reyes , Tania Alejandra Cruz-Chávez , Juan Antonio Gallegos-Marín , Ana María Chávez-Vázquez , Fernando Alatorre-Rendón , Jesús González-Carmona , Bruno Moreno-Medina","doi":"10.1016/j.ram.2024.12.012","DOIUrl":"10.1016/j.ram.2024.12.012","url":null,"abstract":"<div><div>We conducted a prolective cohort study with the aim of estimating the incidence of healthcare-associated infections and identifying associated factors in an intensive care unit in Mexico. Diagnosis of central venous catheter-associated bacteremia, ventilator-associated pneumonia and urinary catheter-associated urinary tract infection was established according to the Centers for Disease Control and Prevention definitions; risk factors were analyzed by logistic regression. Four hundred twenty-six patients who had 486 admissions were studied, 55.9% were male and the median age was 4 years. The healthcare-associated infections incidence rate was 14.8 events/1000 patient-days. The prevalent microorganisms were gram negative bacilli. The factors associated with healthcare-associated infections were chronic conditions (<em>p</em>  ≤0.001).</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 136-141"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial richness assessment in water and sediments in the northern coast of the Yucatan Peninsula 尤卡坦半岛北部海岸水和沉积物中的细菌丰富度评估。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.10.009

Mariela Beatriz Reyes-Sosa , Raúl Valle-Gough , María del Carmen Ponce-Caballero , María Leticia Arena-Ortiz

{"title":"Bacterial richness assessment in water and sediments in the northern coast of the Yucatan Peninsula","authors":"Mariela Beatriz Reyes-Sosa , Raúl Valle-Gough , María del Carmen Ponce-Caballero , María Leticia Arena-Ortiz","doi":"10.1016/j.ram.2024.10.009","DOIUrl":"10.1016/j.ram.2024.10.009","url":null,"abstract":"<div><div>Given the importance of the coastal environments and the multiple ecological services that they provide, it is important to explore and understand the interactions that occur within them. The microbiome is a key factor for the understanding of the dynamics of these fragile sites. A metagenomic study based on the profiling of the 16S ribosomal gene was carried out in order to assess the bacterial diversity present in the northern coastal zone of the Yucatan Peninsula. The results showed that water and sediment samples share some similarities regarding the bacterial genera found, only differing in the quantitative part. Through a PCO (principal coordinates) analysis clear differences between sediment and water samples could be observed. The highest relative diversity was found in wetland and lagoon sediment samples, respectively. It was observed that 3–8% of the total sequence reads belonged to opportunistic genera such as: <em>Vibrio</em> in the sea samples and <em>Capnocytophaga</em> in the other environments. Salinity and pH were the factors which contributed the most to the differences among the communities in the various environments in the coastal zone. There is an important similarity in the sediments across the different environments within the studied coastal zone. The data presented herein contribute to setting a baseline for research in the coastal region of the Yucatan Peninsula.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 171-181"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Broaden properties of ambroxol hydrochloride as an antibiofilm compound 盐酸氨溴索抗生物膜化合物的拓宽性质。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.10.010

María J. González , Michela Lain , Victoria Iribarnegaray , Luciana Robino , Paola Scavone

{"title":"Broaden properties of ambroxol hydrochloride as an antibiofilm compound","authors":"María J. González , Michela Lain , Victoria Iribarnegaray , Luciana Robino , Paola Scavone","doi":"10.1016/j.ram.2024.10.010","DOIUrl":"10.1016/j.ram.2024.10.010","url":null,"abstract":"<div><div>Biofilm-associated microorganisms can cause many infections and are an important cause of resistance to several antimicrobials. The antibiotic crisis has led to a pressing need for new therapeutic tools. Ambroxol is frequently used as a mucolytic agent in respiratory diseases with increased mucus production. In addition, a wide range of properties has been described, including the effect on biofilms. In this work, we evaluate the anti-biofilm effect of ambroxol on four strains with clinical relevance: <em>Proteus mirabilis</em>, <em>Escherichia coli</em>, <em>Staphylococcus aureus</em>, and <em>Acinetobacter baumannii</em>. In vitro, biofilm formation was assessed using the crystal violet quantification technique in microplate and glass coverslip. The inhibition of biofilm formation was evaluated by adding ambroxol at the initial time. Ambroxol hydrochloride was evaluated over the preformed biofilm and live/dead bacteria were quantified. The effect of ambroxol in the ethidium bromide efflux assay and the relative expression of the five major <em>P. mirabilis</em> efflux pump family genes were analyzed. Ambroxol inhibited biofilm formation in all the bacteria tested. Moreover, ambroxol significantly reduces both biofilm biomass and viable bacteria. Ambroxol was able to affect <em>P. mirabilis</em> efflux pumps depending on the concentration used and induced the overexpression of several efflux pump genes. In summary, ambroxol kills planktonic cells, reduce biofilm biomass as it increases cell death, and affect the expression of efflux pumps. Furthermore, it presents a viable alternative for the treatment of biofilm infection alone or in combination with antibiotic therapy.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 114-124"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro and in vivo study of the antifungal activity of extracellular products of cyanobacterium Neowestiellopsis persica strain A1387 against Fusarium wilt disease of cucumber 蓝藻新芽胞外产物A1387对黄瓜枯萎病的体外和体内抑菌活性研究

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.10.011

Bahareh Nowruzi, Hassan Beiranvand

{"title":"In vitro and in vivo study of the antifungal activity of extracellular products of cyanobacterium Neowestiellopsis persica strain A1387 against Fusarium wilt disease of cucumber","authors":"Bahareh Nowruzi, Hassan Beiranvand","doi":"10.1016/j.ram.2024.10.011","DOIUrl":"10.1016/j.ram.2024.10.011","url":null,"abstract":"<div><div><em>Fusarium</em> wilt of cucumber, caused by the fungus <em>Fusarium oxysporum</em>, is a major plant disease that causes significant economic losses. The extensive use of chemical fungicides for its control poses environmental and health risks. Due to growing concerns about the detrimental effects of chemical fungicides, finding safe and effective bio-based alternatives for plant disease control is of high importance. In this study, the potential of <em>Neowestiellopsis persica</em> A1387 cyanobacterial metabolites as a promising substitute for chemical fungicides in controlling this disease was investigated. The antifungal activity of <em>N. persica</em> A1387 cyanobacterial exopolysaccharide (EPS) extract was evaluated against <em>F. oxysporum</em> under <em>in vitro</em> and <em>in vivo</em> conditions. Cucumber plants infected with the fungus were treated with cyanobacterial EPS extract and then assessed for disease severity, antioxidant enzyme activity, and growth parameters. Both biomass and EPS extracts of <em>N. persica</em> A1387 cyanobacteria significantly increased the diameter of the <em>F. oxysporum</em> growth inhibition zone under <em>in vitro</em> conditions. Treatment with cyanobacterial EPS extract resulted in increased dry and fresh weight of stem and roots, and a significant reduction in disease severity and percentage in <em>F. oxysporum</em>-infected plants. Peroxidase, superoxide dismutase (SOD), and catalase enzyme activities in fungus-infected plants treated with cyanobacterial EPS extract were significantly lower on day 42 of infection compared to untreated and infected control plants. These findings demonstrate the potential of <em>N. persica</em> A1387 cyanobacterial extracts as natural and safe alternatives to chemical fungicides for controlling cucumber <em>Fusarium</em> wilt disease.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 182-191"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal and antioxidant potential of Ocimum species against Ascochyta rabiei (Pass) Lab 茜草属植物抗蚜活性及抗氧化活性研究。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2025.02.003

Tooba Shafiq , Khajista Jabeen , Sumera Iqbal , Aisha Umar , Mohamed S. Elshikh , Reem M. Aljowaie , Laurent Dufossé , Rashid Iqbal , Soumya Ghosh

{"title":"Antifungal and antioxidant potential of Ocimum species against Ascochyta rabiei (Pass) Lab","authors":"Tooba Shafiq , Khajista Jabeen , Sumera Iqbal , Aisha Umar , Mohamed S. Elshikh , Reem M. Aljowaie , Laurent Dufossé , Rashid Iqbal , Soumya Ghosh","doi":"10.1016/j.ram.2025.02.003","DOIUrl":"10.1016/j.ram.2025.02.003","url":null,"abstract":"<div><div><em>Cicer arietinum</em> L. is a vital source of nutrients that suffers substantial annual losses due to Ascochyta blight, caused by the plant pathogen <em>Ascochyta rabiei</em>. This study aimed to investigate the antifungal potential of <em>Ocimum tenuiflorum</em> L. and <em>O. basilicum</em> L. shoots (leaves and stems) against <em>A. rabiei</em> (Pass) Lab. <em>In vitro</em> bioassays were conducted using methanolic extracts from leaves and stems at six different concentrations: 1%, 1.5%, 2%, 2.5%, 3%, and 3.5%. A total of eight compounds were identified through gas chromatography–mass spectrometry (GC–MS) analysis. The highest inhibition of <em>A. rabiei</em> growth was achieved with a 3.5% methanolic leaf extract of <em>O. basilicum</em>. Methanolic extracts from <em>O. tenuiflorum</em> shoots also reduced fungal growth by 6.18–73%. Additionally, the n-hexane fraction derived from <em>O. basilicum</em> inhibited fungal growth by 71–76% and was subsequently analyzed using GC–MS. This analysis identified eight compounds: (1) cyclopentane, methyl-, (2) cyclohexane, (3) 2,2-dimethylbutane, (4) 2,3-dimethylbutane, pentane, (5) 2,3-dimethyl-, (6) 2-bromoacetonitrile, (7) alpha-cadinol, and (8) phenylpropanolamine. The antioxidant activity of <em>O. tenuiflorum</em> and <em>O. basilicum</em> shoots was also assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The highest antioxidant activity, 98.58%, was recorded at a 3.5% methanolic stem extract concentration of <em>O. tenuiflorum</em>. The antioxidant activity potential was highest for <em>O. tenuiflorum</em> at 0.729mg/mL, followed by <em>O. basilicum</em> at 0.411mg/mL.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 125-135"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factores de riesgo asociados a la presentación de brucelosis en establecimientos mixtos de caprinos-bovinos de la provincia de Formosa, Argentina [阿根廷福莫萨省混合饲养场（山羊和牛）出现布鲁氏菌病的相关风险因素]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.10.003

Iris Carolina Elías , María Nair Viola , Ana Maria Russo , Marcelo Signorini Porchietto

{"title":"Factores de riesgo asociados a la presentación de brucelosis en establecimientos mixtos de caprinos-bovinos de la provincia de Formosa, Argentina","authors":"Iris Carolina Elías , María Nair Viola , Ana Maria Russo , Marcelo Signorini Porchietto","doi":"10.1016/j.ram.2024.10.003","DOIUrl":"10.1016/j.ram.2024.10.003","url":null,"abstract":"<div><div>Brucellosis is a zoonotic disease with a worldwide distribution that causes significant productive losses and negative impacts on public health. The aim of this work was to identify the risk factors associated with the presence of brucellosis in mixed farms (cattle/goats) in the central-western region of the province of Formosa. Sixty seven mixed farms with 7,855 animals (2,943 cattle and 4,912 goats) were randomly sampled during 2022/2023. The samples were serologically analyzed (BPA + FPA) to determine which animals were positive. Likewise, in each farm, a confidential survey was carried out to evaluate potential associated variables. The statistical analysis was performed using Generalized Linear Mixed Models (GLMM) with binomial distribution, logarithmic link function, and farm as a random variable. The estimated prevalence of brucellosis in mixed farms was 9%, while the joint prevalence per animal (cattle and goats) was 2.75%. The prevalence of brucellosis in cattle and goats was explained by the presence of animals with reproductive signs (<em>odds ratio</em>, OR=40), the occurrence of abortions in the last term of gestation and the birth of weak offspring (OR=5.3), incorrect treatment of abortions (OR=8), the introduction of animals from other farms (OR=5.9), and not having a negative brucellosis certificate (OR=9.6). The management practices conducted in each farm such as preventive measures, sanitation practices for the pens, and prevention measures for the workers, are important for the control of brucellosis.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 152-160"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacto de los marcadores microbiológicos en la evolución de la infección por Clostridioides difficile [微生物标志物在艰难梭菌感染进展中的作用]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.12.003

María Dolores Rojo-Martín , Juan Diego Ruiz-Mesa , Concepción Mediavilla-Gradolph , Mercedes Marín-Arriaza , Alexandra Halbardier-Carretero , José Serrano-Sánchez , Ignacio Márquez-Gómez , Inmaculada de Toro-Peinado , Luis Francisco Caballero-Martínez , Aurora García-Barrionuevo , Begoña Palop-Borrás

{"title":"Impacto de los marcadores microbiológicos en la evolución de la infección por Clostridioides difficile","authors":"María Dolores Rojo-Martín , Juan Diego Ruiz-Mesa , Concepción Mediavilla-Gradolph , Mercedes Marín-Arriaza , Alexandra Halbardier-Carretero , José Serrano-Sánchez , Ignacio Márquez-Gómez , Inmaculada de Toro-Peinado , Luis Francisco Caballero-Martínez , Aurora García-Barrionuevo , Begoña Palop-Borrás","doi":"10.1016/j.ram.2024.12.003","DOIUrl":"10.1016/j.ram.2024.12.003","url":null,"abstract":"<div><div>The main current diagnostic challenge in <em>Clostridioides difficile</em> infection (CDI) is to detect patients with the poorest prognoses. We have studied the association between the presence of toxin in the feces, the amplification of the toxin B gene at low cycles in PCR (Ct), and the detection of the binary toxin or ribotype with severity, recurrence, and poor prognosis. Toxigenic <em>C. difficile</em> has been investigated in adult diarrheal feces, regardless of the clinical request. Glutamate dehydrogenase (GDH), toxin A (TcdA) and toxin B (TcdB) have been assessed by immunoassay and the gene encoding toxin B (tcdB) and the binary toxin (cdtA) by PCR. The toxigenic strains recovered by toxigenic culture were typed by PCR-Ribotyping and subtyped by MLVA (multilocus-variable number tandem DNA repeat analysis). Eighty-two (82) cases of CDI were detected from 677 samples (12.1%). Patients with Ct ≤ 27.5 often presented criteria for severity, recurrence, and poor prognosis (59.3% versus 40.7%, <em>P</em>=.15; 80% versus 20%, <em>P</em>=.10; and 60.3% versus 39.7%, <em>P</em>=.055, respectively). The mean Ct was lower when recurrence was observed (25.48; SD=2.41; <em>P</em>=.058). By logistic regression, the variable most likely to indicate a poor prognosis was Ct ≤ 27.5 (OR=2.663; 95% CI=.983-7.636; <em>P</em>=.059). In summary, the cycle threshold of the PCR for toxin B is a possible marker of severity, recurrence, and poor prognosis in CDI. The diversity of ribotypes observed is remarkable, with no clonal relationship between isolates of the same ribotype.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 142-151"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gluconobacter spp. y Paenibacillus polymyxa causan pudrición en zanahorias comerciales aparentemente sanas [葡萄球菌和多粘类芽孢杆菌会导致看起来健康的商品胡萝卜变质]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.12.009

Thelma J. Parra-Aguilar, María de Jesús Yáñez-Morales, Alejandra Almaraz-Sánchez, Alfonsina Judith Hernández, Sergio Aranda-Ocampo

{"title":"Gluconobacter spp. y Paenibacillus polymyxa causan pudrición en zanahorias comerciales aparentemente sanas","authors":"Thelma J. Parra-Aguilar, María de Jesús Yáñez-Morales, Alejandra Almaraz-Sánchez, Alfonsina Judith Hernández, Sergio Aranda-Ocampo","doi":"10.1016/j.ram.2024.12.009","DOIUrl":"10.1016/j.ram.2024.12.009","url":null,"abstract":"<div><div>Postharvest diseases of carrots are one of the most important concerns in carrot storage, as they affect the shelf life of carrots and cause significant financial losses. In this study, ten commercial carrot samples from supermarkets and local markets in Texcoco, State of Mexico, Mexico, were analyzed for bacterial spoilage after incubation at 28°C in a humidity chamber. Carrots from seven samples developed bacterial spoilage after five days of incubation, of which baby carrots (industrially processed) showed the most severe rot. Twenty-three bacterial strains were isolated from the tissues of spoiled carrots. To identify and characterize the spoilage pathogens, the isolated strains were inoculated into healthy carrots in laboratory tests. Of the 23 strains analyzed, eight caused carrot tissue spoilage. Biochemical and molecular characterization by 16S ribosomal RNA gene sequencing identified <em>Gluconobacter cerinus</em> (1032.2, 1059 and 1070.1), <em>G.</em> <em>kondonii</em> (1027.1), <em>G.</em> <em>wancherniae</em> (1033.1) and <em>Paenibacillus polymyxa</em> (1074.2, 1076 and 1077) as the causal agents of the carrot rot evaluated in this study. In addition, these bacteria showed virulence in other plant pathogenicity tests; <em>Gluconobacter</em> strains induced a hypersensitivity reaction in tobacco leaves and <em>Paenibacillus</em> strains showed pectolytic activity in potato tubers. This study is the first to report <em>G.</em> <em>cerinus</em>, <em>G.</em> <em>kondonii</em>, <em>G.</em> <em>wancherniae</em> and <em>P.</em> <em>polymyxa</em> as causal agents of commercial carrot rot.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 192-201"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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