Revista Argentina de microbiologia最新文献

Microbial consortia as a biocontrol strategy for bacterial blight in basmati rice. 微生物群落作为巴斯马蒂稻白叶枯病的生物防治策略。

IF 2.1 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-10-08 DOI: 10.1016/j.ram.2025.08.003

Bestha Sai Teja, Vishal Gupta, Sushil Kumar Gupta, Ayushi Sharma, Zakir Amin, Fayaz Ahmad Mohiddin, Suhail Ashraf, Mona Saleh Al Tami, Khalid E Hamed

{"title":"Microbial consortia as a biocontrol strategy for bacterial blight in basmati rice.","authors":"Bestha Sai Teja, Vishal Gupta, Sushil Kumar Gupta, Ayushi Sharma, Zakir Amin, Fayaz Ahmad Mohiddin, Suhail Ashraf, Mona Saleh Al Tami, Khalid E Hamed","doi":"10.1016/j.ram.2025.08.003","DOIUrl":"https://doi.org/10.1016/j.ram.2025.08.003","url":null,"abstract":"Basmati rice is in high demand in the world market because of its fine, soft, long and distinctive aromatic grains. Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae, is a destructive disease, resulting in enormous yield loss in basmati rice. Chemical management against BLB is effective but not the preferred approach because of its detrimental effects on humans, environment and ecological disruption. In the present study, seed treatment, seedling treatment and foliar application with microbial antagonists, such as Trichoderma harzianum and Pseudomonas fluorescens, and a consortium encompassing both microorganisms plus Trichoderma asperellum, were assessed against BLB in basmati rice. Among all the bio-formulation treatments, seed treatment with the microbial consortium revealed significant (p<0.05) results, achieving maximum inhibition of BLB incidence, intensity and lesion length of 48.56, 52.42 and 45.41 percent respectively, compared to the untreated control. Simultaneously, the same treatment also resulted in significant (p<0.05) enhancement of growth attributes, such as panicle length (55.83%), plant fresh weight (15.55%), grains per panicle (38.23%), test weight (55.49%) and yield (47.68%). A maximum increase in superoxide dismutase and hydrogen peroxide activities by 81.58 and 84.29 percent respectively over the control was also recorded. The results of the study suggest that the effectiveness of BLB management under field conditions applying the microbial consortium (T. harzianum+T. asperellum+P. fluorescens) was similar to that of the chemical control. Further, efforts are being made to evaluate the biocontrol activity of the microbial consortium in large-scale field experiments and its commercial potential.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuberculosis after solid organ transplantation: Clinical experience in a tertiary hospital from Argentina. 实体器官移植后结核：阿根廷某三级医院的临床经验。

IF 2.1 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-10-07 DOI: 10.1016/j.ram.2025.09.001

Patricia Olga Andres, Elizabeth Madsen, Analía Laura Fernández

引用次数: 0

[Oral fluid PCR monitoring for Aujeszky's disease virus detection in a swine farm]. [某猪场口腔液PCR检测猪瘟病毒]。

IF 2.1 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-10-06 DOI: 10.1016/j.ram.2025.08.001

Melisa G Fossaroli, Mariana Biscia, Melisa V Spadaro, Lilian M Anthony, Paula S Tugores, Javier E Sarradell

引用次数: 0

Extracts from Argentinian native plants exhibit antifungal activity against multidrug-resistant Candida species. 阿根廷本土植物提取物对多重耐药念珠菌具有抗真菌活性。

IF 2.1 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-09-09 DOI: 10.1016/j.ram.2025.07.006

Alejandro N Etchecopaz, Lucia S Di Ciaccio, Katherine Hermida Alava, Reneé H Fortunato, Adriana Salvat, Alejandro D Nusblat, María L Cuestas

{"title":"Extracts from Argentinian native plants exhibit antifungal activity against multidrug-resistant Candida species.","authors":"Alejandro N Etchecopaz, Lucia S Di Ciaccio, Katherine Hermida Alava, Reneé H Fortunato, Adriana Salvat, Alejandro D Nusblat, María L Cuestas","doi":"10.1016/j.ram.2025.07.006","DOIUrl":"https://doi.org/10.1016/j.ram.2025.07.006","url":null,"abstract":"Infections caused by the genus Candida have acquired considerable significance in recent years due to the enhanced susceptibility of immunocompromised hosts. There have been increasing reports of multidrug resistance (MDR) in several Candida species, posing a major hurdle to antifungal therapy. Accordingly, exploring and developing novel anti-Candida agents has become a priority. In this study, we assessed the antifungal activity of seven methanolic extracts from the Argentinian native plants Peltophorum dubium, Schinus areira, Parastrephia quadrangularis and Lantana balansae against clinical isolates of different wild-type and MDR isolates of Candida. Synergism with fluconazole was also evaluated. All plant extracts showed antifungal activities against different Candida species, including MDR isolates such as C. haemulonii. Highly active extracts from these native plants provide promising sources of compounds for potentiating the antifungal effect of fluconazole. Further investigation of the chemical constituents of the extracts and their cytotoxicity is needed to develop plant-derived anti-Candida agents.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial resistance profiles in multidrug-resistant Pseudomonas aeruginosa isolates in a university hospital in Argentina. Activity of new antibiotic combinations. 阿根廷一所大学医院多重耐药铜绿假单胞菌分离株的抗微生物药物耐药性概况。新抗生素组合的活性。

IF 2.1 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-09-08 DOI: 10.1016/j.ram.2025.07.003

Carla Alvarez, Juliana Bello, Carlos Vay, Ángela Famiglietti, Marcela Nastro, Hernán Rodríguez

引用次数: 0

Endophytic Fusarium isolates from Ceratozamia mirandae enhance tomato growth, suppress pathogenic fungi, and induce protection against Botrytis cinerea. mirandae内生镰刀菌对番茄生长有促进作用，对病原菌有抑制作用，对番茄灰霉病有保护作用。

IF 2.1 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-09-06 DOI: 10.1016/j.ram.2025.07.004

Nidia Del C Ríos-De León, Miguel Á Salas-Marina, Vidal Hernández-García, Brenda Del R Saldaña-Morales, Luis A Rodríguez-Larramendi, Rubén Martínez-Camilo, Claudio Ríos-Velasco, Daniel A Pérez-Corral, Sergio Casas-Flores

{"title":"Endophytic Fusarium isolates from Ceratozamia mirandae enhance tomato growth, suppress pathogenic fungi, and induce protection against Botrytis cinerea.","authors":"Nidia Del C Ríos-De León, Miguel Á Salas-Marina, Vidal Hernández-García, Brenda Del R Saldaña-Morales, Luis A Rodríguez-Larramendi, Rubén Martínez-Camilo, Claudio Ríos-Velasco, Daniel A Pérez-Corral, Sergio Casas-Flores","doi":"10.1016/j.ram.2025.07.004","DOIUrl":"https://doi.org/10.1016/j.ram.2025.07.004","url":null,"abstract":"Fungal diseases in agricultural crops cause economic losses, with chemical control being the conventional method to manage them. However, this approach negatively impacts both the environment and human health. This study focused on endophytic fungi isolated from the roots of Ceratozamia mirandae in the Mexican locality of Juan Sabines (Villa Corzo, Chiapas). These fungi were identified morphologically and molecularly, biochemically characterized, and evaluated for their antagonistic activity against Colletotrichum karstii, Neopestalotiopsis sp. and Fusarium oxysporum. Their potential for promoting growth in Arabidopsis thaliana and tomato, as well as protecting against Botrytis cinerea, was also assessed. Fourteen fungal isolates were identified and grouped into six genera: Fusarium, Pestalotiopsis, Trichoderma, Umbelopsis, Nectria and Podospora. Among these Fusarium proliferatum JS311 and F. oxysporum JS239 exhibited strong inhibitory effects against the tested pathogens. Eight isolates were found to produce indole-3-acetic acid, promoting the growth of A. thaliana and tomato plants. Notably, F. oxysporum JS439 and F. solani (JS240, JS256 and JS4101) exhibited additional capabilities, including siderophore production and growth in nitrogen-free media. All Fusarium endophytic isolates induced systemic resistance against B. cinerea in tomato. Endophytic fungi from C. mirandae show promising potential as biofertilizers due to their combined mechanisms of anti-phytopathogenic activity, plant growth promotion, and systemic resistance induction through the production of beneficial metabolites.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Molecular characterization of the ORF5 (GP5) gene from porcine reproductive and respiratory syndrome virus (PRRSV) detected in pig farms in Lima, Peru]. [秘鲁利马猪场猪繁殖与呼吸综合征病毒（PRRSV） ORF5 （GP5）基因的分子特征分析]。

IF 2.1 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-09-04 DOI: 10.1016/j.ram.2025.07.005

Rony Cotaquispe Nalvarte, Miriam Legua Barrios, Patricia Escajadillo Luján, Edgard De la Cruz Vásquez, Jennifer Toskano Hurtado

{"title":"[Molecular characterization of the ORF5 (GP5) gene from porcine reproductive and respiratory syndrome virus (PRRSV) detected in pig farms in Lima, Peru].","authors":"Rony Cotaquispe Nalvarte, Miriam Legua Barrios, Patricia Escajadillo Luján, Edgard De la Cruz Vásquez, Jennifer Toskano Hurtado","doi":"10.1016/j.ram.2025.07.005","DOIUrl":"https://doi.org/10.1016/j.ram.2025.07.005","url":null,"abstract":"The porcine reproductive and respiratory syndrome (PRRS) is an endemic disease in pork-producing regions of the world, and its control remains poor. Rapid identification of PRRSV-1 and PRRSV-2 species is of great importance for molecular epidemiological surveillance of the virus. The objective of this study was the molecular characterization of the ORF5 gene that synthesizes glycosylated protein 5 (GP5) from PRRS virus detected in pig farms in Lima, Perú. One hundred serum samples were used, obtaining 24 PRRSV-2-positive strains confirmed by two molecular techniques. Phylogenetic inference demonstrated the parental relationship with the NADC34 variant, and we achieved a better phylogenetic relationship of the study strains with Peruvian reference isolates in lineage 1 and sublineage 1.5 and pathogenic strains from various parts of the world. Homology analysis showed that strains 25 to 35, 40 to 41, and 43 to 48 obtained high nucleotide homology values of 95-98% and amino acid homology of 92-98%; strains 36, 37, 38, and 39 with high genetic variability with low nucleotide homology values of 83% and amino acid homology of 79%, and strain 42 with homology of 89 and 84% in relation to the NADC34 variant. In conclusion, we demonstrated the effectiveness of the validated in-house primers for identification compared to a commercial kit, in addition to the evolution and dispersion of lineage 1, sublineage 1.5 and the phylogenetic relationship with the NADC34 variant of the PRRSV-2 species in Lima, Perú.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial activity of Streptomyces spp. isolated from bean rhizospheric soil against Xanthomonas sp. 大豆根际土壤链霉菌对黄单胞菌的抑菌活性研究。

IF 2.1 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-08-18 DOI: 10.1016/j.ram.2025.07.002

Jesús Rafael Trinidad-Cruz, Gabriel Rincón-Enríquez, Zahaed Evangelista-Martínez, Evangelina Esmeralda Quiñones-Aguilar

{"title":"Antimicrobial activity of Streptomyces spp. isolated from bean rhizospheric soil against Xanthomonas sp.","authors":"Jesús Rafael Trinidad-Cruz, Gabriel Rincón-Enríquez, Zahaed Evangelista-Martínez, Evangelina Esmeralda Quiñones-Aguilar","doi":"10.1016/j.ram.2025.07.002","DOIUrl":"10.1016/j.ram.2025.07.002","url":null,"abstract":"Species of the genus Streptomyces are a promising strategy for bacterial disease management in agriculture crops. The present study aimed to isolate and identify Streptomyces-like actinobacteria from rhizospheric soil using physical pretreatments and to evaluate their antimicrobial activity against Xanthomonas sp. A rhizospheric soil from a bean plantation was pretreated using dry heat or microwave radiation for isolating actinobacteria. Antimicrobial activity was evaluated using the double agar layer method; 73 isolates exhibiting Streptomyces colony characteristics were obtained from the soil after dry heat pre-treatment series (50 BVBZ) and microwaves (23 BVBZMW); 34 BVBZ (68%) isolates inhibited Xanthomonas sp. growth with 11.2-35.8mm halos in diameter. Fifteen (15) of 23 BVBZMW isolates (65%) recorded inhibition zones ranging from 15.7 to 73.6mm in diameter. The 16S rRNA gene sequence analysis confirmed three isolates with greater antimicrobial activity belonging to the genus Streptomyces. Streptomyces sp. BVBZ 35, BVBZ 47, and BVBZMW 18 shared greater 16S rRNA gene sequence identity with S. monomycini NRRL B-2409T (100%), S. nogalater JCM 4799T (99.38%) and S. leeuwenhoekii C34T (99.45%), respectively. Streptomyces sp. BVBZMW 18, which exhibited the highest antimicrobial activity, could only be cultured after microwave irradiation of the soil. Streptomyces species isolated from rhizospheric soil using physical pretreatments are a potentially novel antimicrobial source for Xanthomonas disease control.","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Severe disseminated nfections in cohabiting people: Genotypic description of methicillin-susceptible Staphylococcus aureus (MSSA) isolate belonging to CC398 clonal complex. 同居人群的严重播散性感染：属于CC398克隆复合体的甲氧西林敏感金黄色葡萄球菌（MSSA）分离物的基因型描述

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-07-24 DOI: 10.1016/j.ram.2025.06.003

Lucía Rago, Gabriela Rubinstein, Carlos Federico Spinelli, María Sol Haim, Tomás Poklépovich Caride, Sabrina Di Gregorio, Marta Mollerach

引用次数: 0

Clinical management of Lancefieldella parvula bacteremia: A case study and review of the literature. 小苗兰斯菲尔德菌血症的临床处理：个案研究及文献回顾。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-07-24 DOI: 10.1016/j.ram.2025.06.004

Cynthia Wenglinski, Delphine Martiny, Michael Watchi, Fabio Silvio Taccone, Charles-Hervé Vacheron

引用次数: 0