Revista Argentina de microbiologia最新文献

{"title":"Prevalence of Demodex spp. in patients with chronic blepharitis","authors":"Viviana Flores ,&nbsp;Marina Ruf ,&nbsp;Silvia Paola Abad Farfan ,&nbsp;Andrea Vanessa Suárez Echávez ,&nbsp;Dolores Pilar Bastard ,&nbsp;Maria Celeste Puga ,&nbsp;Luis Daniel Mazzuoccolo","doi":"10.1016/j.ram.2023.05.005","DOIUrl":"10.1016/j.ram.2023.05.005","url":null,"abstract":"<div><p>Blepharitis is a very common disease in ophthalmology, dermatology and allergy practice. It generally follows a chronic course and is frequently associated with objective and/or subjective symptoms such as epiphora, red eye, dandruff, gritty sensation, itching, burning, photophobia, and blurred vision. The purpose of this study is to analyze the prevalence of <em>Demodex</em> spp. in patients with symptoms of chronic blepharitis. An analytical cross-sectional study was conducted in the period between 2016 and 2020. All patients with symptoms of chronic blepharitis who underwent a parasitological test of eyelashes (Rapitest) in the Dermatology Department of the <em>Hospital Italiano de Buenos Aires</em> were included. Those with previously established blepharitis due to another infectious cause were excluded. We analyzed 972 patients. Sixty percent (n<!--> <!-->=<!--> <!-->585) underwent a positive Rapitest for the presence of <em>Demodex</em> spp. Seventy five percent (n<!--> <!-->=<!--> <!-->728) were women. There were no significant differences in the prevalence associated with sex (<em>p</em> <!-->=<!--> <!-->0.38). Among the patients positive for <em>Demodex</em> spp., 65% (n<!--> <!-->=<!--> <!-->628) were older than 60 years old. The most frequently associated symptom was itching, present in 35% (n<!--> <!-->=<!--> <!-->342). A statistically significant decrease in the number of consultations was observed during the cold months of the year (May–June–July–August). Our results show a high prevalence of <em>Demodex</em> spp. in patients with chronic blepharitis. As its presence reveals a direct association with age, we recommend looking for this parasite in this age group.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 1","pages":"Pages 74-78"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S032575412300055X/pdfft?md5=9580a5c6d93a63f3525313c2f6843ac5&pid=1-s2.0-S032575412300055X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9940067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of plasma anti-lipopolysaccharide (LPS) antibodies against enterohemorrhagic Escherichia coli (EHEC) in asymptomatic kindergarten teachers from Buenos Aires province","authors":"Romina J. Fernandez-Brando ,&nbsp;Flavia Sacerdoti ,&nbsp;María M. Amaral ,&nbsp;Alan M. Bernal ,&nbsp;Marcelo Da Rocha ,&nbsp;Marcela Belardo ,&nbsp;Marina S. Palermo ,&nbsp;Cristina A. Ibarra","doi":"10.1016/j.ram.2023.05.006","DOIUrl":"10.1016/j.ram.2023.05.006","url":null,"abstract":"<div><p>In Argentina, hemolytic uremic syndrome (HUS) caused by EHEC has the highest incidence in the world. EHEC infection has an endemo-epidemic behavior, causing 20–30% of acute bloody diarrhea syndrome in children under 5 years old. In the period 2016–2020, 272 new cases per year were notified to the National Health Surveillance System. Multiple factors are responsible for HUS incidence in Argentina including person-to-person transmission. In order to detect possible EHEC carriers, we carried out a preliminary study of the frequency of kindergarten teachers with anti-LPS antibodies against the most prevalent EHEC serotypes in Argentina. We analyzed 61 kindergarten teachers from 26 institutions from José C. Paz district, located in the suburban area of Buenos Aires province, Argentina. Fifty-one percent of the plasma samples had antibodies against O157, O145, O121 and O103 LPS: 6.4% of the positive samples had IgM isotype (n<!--> <!-->=<!--> <!-->2), 61.3% IgG isotype (n<!--> <!-->=<!--> <!-->19) and 32.3% IgM and IgG (n<!--> <!-->=<!--> <!-->10). Given that antibodies against LPS antigens are usually short-lived specific IgM detection may indicate a recent infection. In addition, the high percentage of positive samples may indicate a frequent exposure to EHEC strains in the cohort studied, as well as the existence of a large non-symptomatic population of adults carrying pathogenic strains that could contribute to the endemic behavior through person-to-person transmission. The improvement of continuous educational programs in kindergarten institutions could be a mandatory measure to reduce HUS cases not only in Argentina but also globally.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 1","pages":"Pages 25-32"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000561/pdfft?md5=d85b90f8e9aa64cd342e2669c9a1f6a0&pid=1-s2.0-S0325754123000561-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10229462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caracterización molecular de Escherichia coli diarreogénica proveniente de población pediátrica ambulatoria con diarrea, atendida en dos hospitales de Buenos Aires, Argentina","authors":"Nora Beatriz Molina ,&nbsp;Sebastián Oderiz ,&nbsp;Marisa Ana López ,&nbsp;Juan Ángel Basualdo ,&nbsp;Mónica Delfina Sparo","doi":"10.1016/j.ram.2023.06.002","DOIUrl":"10.1016/j.ram.2023.06.002","url":null,"abstract":"<div><p>Diarrheagenic <em>Escherichia coli</em> comprises a heterogeneous group of pathotypes or pathogenic variants that share phenotypic characteristics with marked differences in virulence genes, colonization sites, pathogenesis, clinical presentation, and epidemiology of infection. The most studied pathotypes are Shiga toxin-producing <em>E.</em> <em>coli</em> (STEC), enterotoxigenic <em>E.</em> <em>coli</em> (ETEC), enteropathogenic <em>E.</em> <em>coli</em> (EPEC), enteroaggregative <em>E.</em> <em>coli</em> (EAEC), and enteroinvasive <em>E.</em> <em>coli</em> (EIEC). The objective of the study was to characterize the isolates of diarrheagenic <em>E.</em> <em>coli</em> from an outpatient pediatric population with diarrhea attended in two public hospitals from Buenos Aires, Argentina. Diarrheagenic <em>E.</em> <em>coli</em> pathotypes were investigated by amplifying characteristic virulence gene fragments: intimin (<em>eae</em>), heat-labile toxin (<em>lt</em>), heat-stable toxins (<em>stp</em>, <em>sth</em>), invasion plasmid antigen H (<em>ipaH</em>), transcriptional activator R (<em>aggR</em>) and Shiga toxins (<em>stx1</em>, <em>stx2</em>). Molecular subtyping of isolates was performed using PFGE (XbaI). Diarrheagenic <em>E.</em> <em>coli</em> was detected in 14% (84/601) of cases. The EAEC pathotype was prevalent, while ETEC, STEC, EPEC and EIEC were found in a lower proportion. EAEC isolates exhibited a high degree of genetic diversity. All pathotypes were found in children under 5<!--> <!-->years of age, while only EAEC, EIEC and ETEC were detected in the older population. Future studies that include the characterization of isolates from a greater number of genes and populations from other geographical areas will be necessary to determine the relevance of diarrheagenic <em>E.</em> <em>coli</em> in Argentina.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 1","pages":"Pages 8-15"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000536/pdfft?md5=78f54a484a5c420995c5c6ecfb040477&pid=1-s2.0-S0325754123000536-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9886098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feline sporotrichosis due to Sporothrix globosa in the Metropolitan region of Buenos Aires: A case study","authors":"Alejandro N. Etchecopaz ,&nbsp;Katherine Hermida Alava ,&nbsp;Miguel Scarpa ,&nbsp;Adriana Duchene ,&nbsp;Diego García Re ,&nbsp;María Luján Cuestas","doi":"10.1016/j.ram.2023.07.001","DOIUrl":"10.1016/j.ram.2023.07.001","url":null,"abstract":"<div><p>We describe here the first case of feline sporotrichosis caused by <em>Sporothrix globosa</em>, occurring outside the epizootic area of Buenos Aires province, Argentina. Unlike cases reported with <em>Sporothrix brasiliensis</em>, on this occasion there was no clinical or serological evidence of zoonotic transmission through scratches or bites from the sick cat to the attending veterinarian or the person responsible for its care. This report aimed to improve the knowledge about the pathogenic profile of <em>S. globosa</em>.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 1","pages":"Pages 4-7"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000573/pdfft?md5=42b8b97cc09797198665ea00ece37acc&pid=1-s2.0-S0325754123000573-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10406304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth, tolerance, and enzyme activities of Trichoderma strains in culture media added with a pyrethroids-based insecticide","authors":"Caliope Mendarte-Alquisira,&nbsp;Alejandro Alarcón,&nbsp;Ronald Ferrera-Cerrato","doi":"10.1016/j.ram.2023.06.004","DOIUrl":"10.1016/j.ram.2023.06.004","url":null,"abstract":"<div><p>The application of pyrethroids and carbamates represents an environmental risk and may exert adverse effects on beneficial microorganisms such as <em>Trichoderma</em>, which contribute to the biocontrol of several fungal phytopathogens. This research evaluated the tolerance of several strains of <em>Trichoderma</em> to a selected culture medium contaminated with a commercial insecticide (H24®) composed of pyrethroids, permethrin and prallethrin, and carbamate propoxur, and determined the influence of this insecticide on the release of enzymes such as chitinases, peroxidases, and endoglucanases by a consortium of selected <em>Trichoderma</em> strains grown in liquid culture medium. Four out of 10 <em>Trichoderma</em> strains showed tolerance to 200<!--> <!-->ppm (∼48.3% of growth) of the commercial insecticide after 96<!--> <!-->h of exposure to a contaminated solid medium. After eight days of growth in liquid culture, the insecticide enhanced extracellular protein content and peroxidase activities in the <em>Trichoderma</em> consortium but decreased both chitinase and glucanase activities. These fungal responses should be considered when implementing strategies that combine alternative pesticides and fungal biocontrollers for managing fungal phytopathogens.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 1","pages":"Pages 79-89"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000597/pdfft?md5=4feb8875011e336f3a3fbce993f62818&pid=1-s2.0-S0325754123000597-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10466689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macroencapsulation of Limosilactobacillus reuteri DSPV002C as nutritional supplement for piglets: Storage stability and survival in gastrointestinal conditions","authors":"Jorge Alberto Zimmermann ,&nbsp;Noelí Sirini ,&nbsp;Carolina Raquel Olivero ,&nbsp;María Sol Renna ,&nbsp;Marcelo Lisandro Signorini ,&nbsp;María Virginia Zbrun ,&nbsp;Laureano Sebastián Frizzo ,&nbsp;Lorena Paola Soto","doi":"10.1016/j.ram.2023.07.005","DOIUrl":"10.1016/j.ram.2023.07.005","url":null,"abstract":"<div><p>The aim of this study was to evaluate the protective effect of the encapsulation of <em>Limosilactobacillus reuteri</em> DSPV002C in macrocapsules made from industrial materials during production, storage and under simulated gastrointestinal conditions <em>in vitro</em> and <em>in vivo</em>. The production of macrocapsules involved the evaluation of different wall materials (matrix), namely, gelatin and pregelatinized starch, different inoculums, matrix ratios, and diverse cryoprotectants (whey permeate and maltodextrin). The different macrocapsules were arranged in molds of similar size to pig pelleted food and lyophilized. Then, the viability of the macrocapsules was assessed over time during storage at different temperatures (freezing, refrigeration and room temperature) and atmospheres (vacuum and non-vaccum). The macrocapsules with 10% w/v gelatin<!--> <!-->+<!--> <!-->5% w/v pregelatinized starch, permeated (10%, w/v), with a 9:1 inoculum:matrix ratio (GS7.5P9), stored under freezing conditions and vacuum, exhibited the highest viability of <em>L. reuteri</em> DSPV002C (9.3 log CFU/cap until 210 d). Under simulated gastrointestinal conditions, the encapsulated inoculum showed less viability loss (0.58<!--> <!-->±<!--> <!-->0.09 log CFU/ml, 26.53%), compared to the free culture (1.56<!--> <!-->±<!--> <!-->0.16 log CFU/ml, 2.85%). Finally, by administering GS7.5P9 to pigs, the tolerance of the bacteria to the gastrointestinal environment was verified, with viable counts equal to or greater than 3.72 log CFU/g of fecal matter throughout the trial. In this study, a high-density carrier probiotic macrocapsule of <em>L. reuteri</em> DSPV002C was obtained, which displayed a long shelf life, a suitable shape to be included in pig feed and an adequate survival of viable cells at the site of action.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 1","pages":"Pages 90-101"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000822/pdfft?md5=c6ecab413d42605d383ba4cb98193446&pid=1-s2.0-S0325754123000822-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71485530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Similarities of Geobacillus bacteria based on their profiles of antimicrobial susceptibility in milk samples","authors":"Orlando G. Nagel,&nbsp;Maria L. Gasparotti,&nbsp;Selva I. Machado,&nbsp;Rafael L. Althaus","doi":"10.1016/j.ram.2023.07.003","DOIUrl":"10.1016/j.ram.2023.07.003","url":null,"abstract":"<div><p>The genus <em>Geobacillus</em> is composed of thermophilic bacteria that exhibit diverse biotechnological potentialities. Specifically, <em>Geobacillus stearothermophilus</em> is included as a test bacterium in commercial microbiological inhibition methods, although it exhibits limited sensitivity to aminoglycosides, macrolides, and quinolones. Therefore, this article evaluates the antibiotic susceptibility profiles of five test bacteria (<em>G. stearothermophilus</em> subsp. <em>calidolactis</em> C953, <em>Geobacillus thermocatenulatus</em> LMG 19007, <em>Geobacillus thermoleovorans</em> LMG 9823, <em>Geobacillus kaustophilus</em> DSM 7263 and <em>Geobacillus vulcani</em> 13174). For that purpose, the minimum inhibitory concentrations (MICs) of 21 antibiotics were determined in milk samples for five test bacteria using the radial diffusion microbiological inhibition method. Subsequently, the similarities between bacteria and antibiotics were analyzed using cluster analysis. The dendrogram of this multivariate analysis shows an association between a group formed by <em>G. thermocatenulatus</em> and <em>G. stearothermophilus</em> and another by <em>G. thermoleovorans</em>, <em>G. kaustophilus</em> and <em>G. vulcani</em>. Finally, future microbiological methods could be developed in microtiter plates using <em>G. thermocatenulatus</em> as test bacterium, as it exhibits similar sensitivities to <em>G. stearothermophilus</em>. Conversely, <em>G. vulcani</em>, <em>G. thermoleovorans</em> and <em>G. kaustophilus</em> show higher MICs than <em>G. thermocatenulatus</em>.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 1","pages":"Pages 102-111"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000615/pdfft?md5=0dccb4197d8cfe904e7f1af9fc0c047d&pid=1-s2.0-S0325754123000615-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10222827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asociación del consumo de drogas ilícitas en mujeres embarazadas y sífilis congénita en un Hospital Público de México","authors":"Lucía Miramontes-Buiza ,&nbsp;Juan Carlos Lona-Reyes ,&nbsp;Rene Oswaldo Pérez-Ramírez ,&nbsp;Diego Magallón-Picazo ,&nbsp;Araceli Cordero-Zamora ,&nbsp;Larissa María Gómez-Ruiz ,&nbsp;Tania Alejandra Cruz-Chávez","doi":"10.1016/j.ram.2023.07.002","DOIUrl":"10.1016/j.ram.2023.07.002","url":null,"abstract":"<div><p>We conducted a nested case–control study within a cohort with the aim of studying the association between illicit drug use and congenital syphilis (CS). Cases were diagnosed based on treponemal and non-treponemal tests conducted both in the mother and the newborn (NB). Multivariate analysis with logistic regression was performed. A total of 6171 births with a mean gestational age of 37.8 weeks were recorded and 62 CS events were diagnosed (incidence 10.5 events/1000 NB). Associated maternal factors were illicit drug use (OR 14.08, 95% <span>C</span>I 1.19–166.6), &lt;5 prenatal visits (OR 2.9, 95% CI 1.12–7.53), more than two sexual partners (OR 3.76, 95% CI 1.62–8.71) and professional education level (OR 0.06, 95% CI 0.005–0.85). Among the mothers of the cases presented, the prevalence of illicit drug use was 22.6% and the most frequent drugs were methamphetamines and cannabis.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 1","pages":"Pages 69-73"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000585/pdfft?md5=603170c7cc735f1b1d75dd5fac1e88f8&pid=1-s2.0-S0325754123000585-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10036679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological control of foodborne pathogens by lactic acid bacteria: A focus on juice processing industries","authors":"María Clara Tarifa ,&nbsp;María del Rosario Agustín ,&nbsp;Lorena Inés Brugnoni","doi":"10.1016/j.ram.2023.04.001","DOIUrl":"10.1016/j.ram.2023.04.001","url":null,"abstract":"<div><p>The use of lactic acid bacteria (LAB) in foods as biocontrol agents against foodborne pathogens has become increasingly known. Under the premise that controlling the adhesion of microorganisms to food contact surfaces is an essential step for meeting the goals of food processing, the aim of this work was to investigate the inhibitory and anti-biofilm effectiveness of <em>Lactobacillus rhamnosus</em> GG (ATCC 53103) and <em>Lactobacillus casei</em> (ATCC 393) against <em>Escherichia coli</em> O157:H7, <em>Salmonella enterica</em> and <em>Listeria monocytogenes</em>. <em>Lactobacillus</em> strains (10⁸ <!-->CFU/ml) and pathogens (10⁴ <!-->CFU/ml) were evaluated to monitor LAB anti-adhesive and antibiofilm effect, in two main scenarios: (i) co-adhesion and (ii) pathogen incorporation to stainless steel surfaces with a protective biofilm of <em>Lactobacillus</em> cells. In (i) the predominant effect was observed in <em>L. rhamnosus</em> against <em>S. enterica</em> and <em>L. monocytogenes,</em> whereas in (ii) both LAB significantly reduced the number of pathogenic adherent cells. The effect of pre-established LAB biofilms was more successful in displacing the three pathogens than when they were evaluated under co-adhesion. These findings show that both LAB can be considered good candidates to prevent or inhibit the adhesion and colonization of <em>L. monocytogenes</em>, <em>S. enterica</em> and <em>E. coli</em> O157:H7 on surfaces and conditions of relevance for juice processing industries, offering alternatives for improving the safety and quality of fruit-based products.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"55 4","pages":"Pages 378-386"},"PeriodicalIF":1.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000299/pdfft?md5=e02e9cc50d806ba75cb0e2ae728abcbc&pid=1-s2.0-S0325754123000299-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9612346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of serologically active Helicobacter pylori antigens related to alterations in serum pepsinogen levels","authors":"Rodolfo González Segovia ,&nbsp;Yolanda Romo Lozano ,&nbsp;Martín Gerardo Rodríguez ,&nbsp;Angelina Lizbeth Montañez Flores ,&nbsp;Juan González Macías","doi":"10.1016/j.ram.2023.04.003","DOIUrl":"10.1016/j.ram.2023.04.003","url":null,"abstract":"<div><p>Gastric adenocarcinoma is associated with <em>Helicobacter pylori</em> infection. The transition to a carcinogenic process is preceded by glandular atrophy and serum levels of pepsinogen I and II (PGI and PGII) correlate with this type of gastric lesions. Possible associations of serum PG levels in relation to the frequency of serological activity against <em>H. pylori</em> antigens were studied. Serum samples from patients with gastric pathology associated with <em>H. pylori</em> (n<!--> <!-->=<!--> <!-->26) and asymptomatic individuals as controls (n<!--> <!-->=<!--> <!-->37) were used. Seroactive antigens were identified by immunoblot using a protein extract of <em>H. pylori</em>. The antibody titers anti-<em>H. pylori</em> and the concentration of PGs in serum was determined by ELISA. Thirty-one seroactive antigens were identified, nine of which exhibited a differential frequency between both groups (116.7, 68.8, 61.9, 54.9, 45.6, 38.3, 36.5, 33.8 and 30.1<!--> <!-->kDa) and only 3 were related to altered levels of PGs in serum. In the control group, the seropositivity of the 33.8<!--> <!-->kDa antigen was related to an increase in PGII, while the 68.8<!--> <!-->kDa antigen was related to normal PG values (decreased PGII and elevated PGI/PGII levels) indicating that seropositivity to this antigen could be a protective factor to gastric pathology. The seropositivity of the 54.9<!--> <!-->kDa antigen was related to altered values of PGs indicative of inflammation and gastric atrophy (increased in PGII and decreased in PGI/PGII). The identification of serum alterations in pepsinogen levels related to seropositivity to <em>H. pylori</em> 33.8, 54.9 and 68.8<!--> <!-->kDa antigens sets a precedent for further study as possible prognostic serological biomarkers.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"55 4","pages":"Pages 355-365"},"PeriodicalIF":1.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000317/pdfft?md5=109cb19883636f167d3f52a1bffb2cbe&pid=1-s2.0-S0325754123000317-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10074326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}