Revista Argentina de microbiologia最新文献_第9页

Transcriptional study of genes involved in the passage from teliospore to hyphae stage in the fungus Thecaphora frezii, the causal agent of peanut smut 花生烟粉虱病原真菌 Thecaphora frezii 从端孢子到菌丝阶段所涉及基因的转录研究。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.10.002

María S. Díaz , Néstor W. Soria , Ana C. Figueroa , Pablo Yang , Esteban H. Badariotti , Valeria R. Alasino , Pablo Vélez , Dante M. Beltramo

{"title":"Transcriptional study of genes involved in the passage from teliospore to hyphae stage in the fungus Thecaphora frezii, the causal agent of peanut smut","authors":"María S. Díaz , Néstor W. Soria , Ana C. Figueroa , Pablo Yang , Esteban H. Badariotti , Valeria R. Alasino , Pablo Vélez , Dante M. Beltramo","doi":"10.1016/j.ram.2023.10.002","DOIUrl":"10.1016/j.ram.2023.10.002","url":null,"abstract":"<div>Peanuts (Arachis hypogaea L.) are among the most important leguminous crops in Argentina. During the growing season, they are frequently attacked by fungal diseases, including Thecaphora frezii. The spores of T. frezii are structures that confer resistance to this phytopathogen. The transition from teliospore to hypha is a characteristic process of some fungi, which is essential for completing their life cycle. Using the transcriptomes of teliospores and hyphae of T. frezii, we aimed to identify genes that were differentially expressed during this transition, and we found 134 up-regulated and 66 down-regulated genes, which would participate in different cellular processes such as: (a) cell cycle and DNA processing; (b) cell fate; (c) rescue, defense and cellular virulence; (d) detoxification by CYP450; (e) energy; (f) nutrient interaction and nutritional adaptation; (g) metabolism; (g) proteins with binding functions or cofactor requirements; (h) stress, cell differentiation and biogenesis of cell components; and (i) transport, cell communication and transcription. The identification of genes in T. frezii and their expression levels during different stages of differentiation could contribute to our understanding of the biological mechanisms in this fungus.</div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 175-186"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000038/pdfft?md5=11d862ad8a5aea638a6c5f4342331d5a&pid=1-s2.0-S0325754124000038-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139712941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbapenemen-resistant Enterobacterales: An issue of global concern 耐碳青霉烯类肠杆菌：全球关注的问题

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2024.05.001

Marcela Nastro

引用次数: 0

Prevalencia y distribución geográfica de las enfermedades de transmisión sexual de los bovinos en la provincia de Formosa, Argentina [阿根廷福尔摩沙省牛性传播疾病的流行率和地理分布]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.07.004

María Nair Viola , Iris Carolina Elías , Marcelo Signorini , Ana Ines Molineri , Ana María Russo , Patricia Andrea Zimmer , Laura Analía Lozina , Juana Noemí Gimenez

{"title":"Prevalencia y distribución geográfica de las enfermedades de transmisión sexual de los bovinos en la provincia de Formosa, Argentina","authors":"María Nair Viola , Iris Carolina Elías , Marcelo Signorini , Ana Ines Molineri , Ana María Russo , Patricia Andrea Zimmer , Laura Analía Lozina , Juana Noemí Gimenez","doi":"10.1016/j.ram.2023.07.004","DOIUrl":"10.1016/j.ram.2023.07.004","url":null,"abstract":"<div>Bovine genital campylobacteriosis (BGC) and bovine trichomonosis (BT) are sexually transmitted diseases (STDs) that affect bovine breeding herds, decreasing their reproductive efficiency. The objective of this work was to estimate the prevalence of these diseases and their temporal-spatial distribution in the province of Formosa, Argentina. The cross-sectional study conducted between 2018 and 2021 included a total of 15,571 bulls, inter-herd prevalence being 29.62% and 17.23% for BGC and BT, respectively. The prevalence of positive animals was 2.05% for BGC and 0.43% for BT. The temporal-spatial analysis of BGC showed two distinct spatial groupings, one group had a low risk of contracting the disease (RR = 0.13; p < 0.001; 2018–2021) while the other group had a high risk (RR = 2.84; p < 0.001; 2020–2021). BT had a high-risk group for the disease (RR = 35.24; p < 0.001; 2019). This study shows that STDs are endemic in the region, providing updated and valuable information as a tool for the health management of these diseases.</div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 147-152"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000627/pdfft?md5=756acbfa3de90a2c6d1cbd1f7ba2b321&pid=1-s2.0-S0325754123000627-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72015171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The microPIXE technique to understand the distribution of heavy metals in arbuscular mycorrhizal symbiosis 利用微PIXE技术了解重金属在假根菌根共生中的分布。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.12.006

M. Statello , R.P. Colombo , E.M. de la Fournière , M.E. Debray , A.M. Godeas , V.A. Silvani

引用次数: 0

Carpogenic germination of Sclerotinia sclerotiorum sclerotia in Brassica oleracea var gemmifera (VN: Brussels sprouts) in North Patagonia, Argentina 阿根廷北巴塔哥尼亚甘蓝菜（Brassica oleracea var gemmifera）（VN：球芽甘蓝）中硬粒菌的鲤鱼发芽。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.12.005

A.G. Scarso , M.C. Sosa , M.J. Ousset , M.C. Lutz

引用次数: 0

In house-development of a rapid immunochromatographic test for the detection of Escherichia coli in urine samples 内部开发用于检测尿液样本中大肠杆菌的快速免疫层析检测法

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.10.001

Paula Da Cunda , Amy Mónaco , María Moreno , María José Gonzalez , Paola Scavone , Luciana Robino

{"title":"In house-development of a rapid immunochromatographic test for the detection of Escherichia coli in urine samples","authors":"Paula Da Cunda , Amy Mónaco , María Moreno , María José Gonzalez , Paola Scavone , Luciana Robino","doi":"10.1016/j.ram.2023.10.001","DOIUrl":"10.1016/j.ram.2023.10.001","url":null,"abstract":"<div>Urinary tract infections (UTIs) are a common health concern. Urine culture is the “gold standard” for UTI diagnosis but takes 48h. Rapid methods like dipstick tests are used as point-of-care tests. However, their sensitivity and specificity are variable. In this work, a rapid immunochromatographic test (IT) for detecting Escherichia coli in urine was developed, and its performance was evaluated in urine samples from patients with suspected UTI. The “universal lateral flow assay kit” was employed using an E. coli capture antibody. One hundred and five (105) urine samples were analyzed using the IT, dipstick test, and urine culture. The sensitivity of the IT was 74.5%, specificity 88.9%, positive predictive value (PPV) 86.3%, and negative predictive value (NPV) 78.7%. The combination of the IT with the dipstick test increases sensitivity to 94.1%, specificity to 66.7%, PPV to 72.7%, and NPV to 92.3%. Using the IT for detecting E. coli in urine could be a valuable technique for UTI screening, showing better specificity and diagnostic precision but lower sensitivity than the dipstick test. Based on these results, we propose that the combined use of both screening techniques would allow a rapid and more precise diagnosis of UTI, rationalizing the indication for empirical antibiotics.</div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 140-146"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000846/pdfft?md5=5dd878bb5bfa171fb4bec9b4b72511e8&pid=1-s2.0-S0325754123000846-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138558474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory action of antibiotics on Kluyveromyces marxianus 抗生素对 Kluyveromyces marxianus 的抑制作用。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.12.004

Rafael Lisandro Althaus, Orlando Guillermo Nagel, Dafna Eluk

{"title":"Inhibitory action of antibiotics on Kluyveromyces marxianus","authors":"Rafael Lisandro Althaus, Orlando Guillermo Nagel, Dafna Eluk","doi":"10.1016/j.ram.2023.12.004","DOIUrl":"10.1016/j.ram.2023.12.004","url":null,"abstract":"<div>A bioassay containing Kluyveromyces marxianus in microtiter plates was used to determine the inhibitory action of 28 antibiotics (aminoglycosides, beta-lactams, macrolides, quinolones, tetracyclines and sulfonamides) against this yeast in whey. For this purpose, the dose–response curve for each antibiotic was constructed using 16 replicates of 12 different concentrations of the antibiotic. The plates were incubated at 40°C until the negative samples exhibited their indicator (5–7h). Subsequently, the absorbances of the yeast cells in each plate were measured by the turbidimetric method (λ   <!-->nm) and the logistic regression model was applied. The concentrations causing 10% (IC10) and 50% (IC50) of growth inhibition of the yeast were calculated. The results allowed to conclude that whey contaminated with cephalosporins, quinolones and tetracyclines at levels close to the Maximum Residue Limits inhibits the growth of K. marxianus. Therefore, previous inactivation treatments should be implemented in order to re-use this contaminated whey by fermentation with K. marxianus.</div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 134-139"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000063/pdfft?md5=8cbf2798c3ebf9d3e8961caef23ab7af&pid=1-s2.0-S0325754124000063-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterization of Moraxella bovis and Moraxella bovoculi Uruguayan strains isolated from calves with infectious bovine keratoconjunctivitis 从患有传染性牛角膜结膜炎的犊牛身上分离出的牛莫拉菌和牛莫拉菌乌拉圭株的基因组特征。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.12.003

Lucía Bilbao , Sofía Acquistapace , Ana Umpiérrez , Pablo Smircich , Pablo Alonzo , José R. Sotelo-Silveira , Pablo Zunino

{"title":"Genomic characterization of Moraxella bovis and Moraxella bovoculi Uruguayan strains isolated from calves with infectious bovine keratoconjunctivitis","authors":"Lucía Bilbao , Sofía Acquistapace , Ana Umpiérrez , Pablo Smircich , Pablo Alonzo , José R. Sotelo-Silveira , Pablo Zunino","doi":"10.1016/j.ram.2023.12.003","DOIUrl":"10.1016/j.ram.2023.12.003","url":null,"abstract":"<div>Infectious bovine keratoconjunctivitis (IBK) is an ocular disease that affects bovines and has significant economic and health effects worldwide. Gram negative bacteria Moraxella bovis and Moraxella bovoculi are its main etiological agents. Antimicrobial therapy against IBK is often difficult in beef and dairy herds and, although vaccines are commercially available, their efficacy is variable and dependent on local strains. The aim of this study was to analyze for the first time the genomes of Uruguayan clinical isolates of M. bovis and M. bovoculi. The genomes were de novo assembled and annotated; the genetic basis of fimbrial synthesis was analyzed and virulence factors were identified. A 94% coverage in the reference genomes of both species, and more than 80% similarity to the reference genomes were observed. The mechanism of fimbrial phase variation in M. bovis was detected, and the tfpQ orientation of these genes confirmed, in an inversion region of approximately 2.18kb. No phase variation was determined in the fimbrial gene of M. bovoculi. When virulence factors were compared between strains, it was observed that fimbrial genes have 36.2% sequence similarity. In contrast, the TonB-dependent lactoferrin/transferrin receptor exhibited the highest percentage of amino acid similarity (97.7%) between strains, followed by cytotoxins MbxA/MbvA and the ferric uptake regulator. The role of these virulence factors in the pathogenesis of IBK and their potential as vaccine components should be explored.</div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 165-174"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S032575412400004X/pdfft?md5=0b45766957c04db35ff5e432bc7e3802&pid=1-s2.0-S032575412400004X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139973269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial diversity using metagenomics of 16s rDNA in water kefir, an innovative source of probiotics for bee nutrition 利用 16s rDNA 元基因组学研究水酸乳中的细菌多样性--蜜蜂营养的创新益生菌来源

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.12.002

María A. Rodríguez , Leticia A. Fernández , Marina L. Díaz , Cristian A. Gallo , Miguel Corona , Jay D. Evans , Francisco J. Reynaldi

{"title":"Bacterial diversity using metagenomics of 16s rDNA in water kefir, an innovative source of probiotics for bee nutrition","authors":"María A. Rodríguez , Leticia A. Fernández , Marina L. Díaz , Cristian A. Gallo , Miguel Corona , Jay D. Evans , Francisco J. Reynaldi","doi":"10.1016/j.ram.2023.12.002","DOIUrl":"10.1016/j.ram.2023.12.002","url":null,"abstract":"<div>Water kefir is a sparkling, slightly acidic fermented beverage made from sugar, water, and water kefir grains, which are a mixture of yeast and bacteria. These grains produce a variety of fermentation compounds such as lactic acid, acetaldehyde, acetoin, ethanol and carbon dioxide. In this study, a high-throughput sequencing technique was used to characterize the bacterial composition of the original water kefir from which potential probiotics were obtained. We studied the bacterial diversity of both water kefir grains and beverages. DNA was extracted from three replicate samples of both grains and beverages using the Powerlyzer Microbial Kit. The hypervariable V1–V2 region of the bacterial 16S ribosomal RNA gene was amplified to prepare six DNA libraries. Between 1.4M and 2.4M base-pairs were sequenced for the library. In total, 28721971 raw reads were obtained from all the samples. Estimated species richness was higher in kefir beverage samples compared to grain samples. Moreover, a higher level of microbial alpha diversity was observed in the beverage samples. Particularly, the predominant bacteria in beverages were Anaerocolumna and Ralstonia, while in grains Liquorilactobacillus dominated, with lower levels of Leuconostoc and Oenococcus.Although the bacterial diversity in kefir grains was low because only three genera were the most represented, all of them are LAB bacteria with the potential to serve as probiotics in the artificial feeding of bees.</div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 191-197"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000026/pdfft?md5=6bdb5a46b2c43ca9fbccc5165b8c44d1&pid=1-s2.0-S0325754124000026-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139560719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MVA vector expressing the F protein of bovine respiratory syncytial virus is immunogenic in systemic and mucosal immunization routes 表达牛呼吸道合胞病毒 F 蛋白的 MVA 载体在全身和粘膜免疫途径中具有免疫原性

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-04-01 DOI: 10.1016/j.ram.2023.07.006

Alejandra Ferella , Marina Mozgovoj , Débora Garanzini , María José Dus Santos , Gabriela Calamante , María Paula Del Médico Zajac

{"title":"The MVA vector expressing the F protein of bovine respiratory syncytial virus is immunogenic in systemic and mucosal immunization routes","authors":"Alejandra Ferella , Marina Mozgovoj , Débora Garanzini , María José Dus Santos , Gabriela Calamante , María Paula Del Médico Zajac","doi":"10.1016/j.ram.2023.07.006","DOIUrl":"10.1016/j.ram.2023.07.006","url":null,"abstract":"<div>Bovine respiratory syncytial virus (BRSV) affects both beef and dairy cattle, reaching morbidity and mortality rates of 60–80% and 20%, respectively. The aim of this study was to obtain a recombinant MVA expressing the BRSV F protein (MVA-F) as a vaccine against BRSV and to evaluate the immune response induced by MVA-F after systemic immunization in homologous and heterologous vaccination (MVA-F alone or combined with a subunit vaccine), and after intranasal immunization of mice. MVA-F administered by intraperitoneal route in a homologous scheme elicited levels of neutralizing antibodies similar to those obtained with inactivated BRSV as well as better levels of IFN-γ secretion. In addition, nasal administration of MVA-F elicited local and systemic immunity with a Th1 profile. This study suggests that MVA-F is a good candidate for further evaluations combining intranasal and intramuscular routes, in order to induce local and systemic immune responses, to improve the vaccine efficacy against BRSV infection.</div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 125-133"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000834/pdfft?md5=2c178a5b674c409dc03246bf2844c9f8&pid=1-s2.0-S0325754123000834-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139031116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0