Revista Argentina de microbiologia最新文献

{"title":"Caracterización molecular de aislamientos de Listeria monocytogenes de origen humano y alimentario en Argentina, 2018-2023","authors":"","doi":"10.1016/j.ram.2024.03.002","DOIUrl":"10.1016/j.ram.2024.03.002","url":null,"abstract":"<div><p>Human listeriosis is an infectious disease caused by <em>Listeria monocytogenes</em>. The invasive form of this disease leads to a high rate of hospitalizations and fatality. The main mode of transmission is through contaminated ready-to-eat foods such as dairy, vegetables and meat products. The knowledge of the diversity and population dynamics of isolates collected from human and food sources is essential for the detection of clusters and the identification of common sites of infection. The aim of this study was the molecular characterization of <em>L. monocytogenes</em> isolates in Argentina. We sequenced a total of 63 isolates, 35 from human and 28 from food sources, collected between 2018 and 2023. Our genomic study divided the isolates into two lineages, four serogroups, 17 sequence types and 15 clonal complexes (CCs). The hypervirulent clone CC1 (lineage I; serogroup IVb) predominated in human and food samples. The phylogenomic analysis showed a high and possible epidemiological relationship between isolates from human and/or food sources, suggesting the presence of transmission chains in our country. These findings highlight the need to strengthen genomic surveillance of <em>L. monocytogenes</em> in Argentina. The identification of geographic distribution and characteristics of predominant and emerging clones from human and food sources might help to focus action plans and public health policies better directed at the control and prevention of listeriosis.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 329-335"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000427/pdfft?md5=30bf52e2d9d8339ac85d9f6231190014&pid=1-s2.0-S0325754124000427-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obtaining more contaminant-resistant variants from a native Chlorella vulgaris strain","authors":"Andrea G. Trentini ,&nbsp;Uriel D. Salvio ,&nbsp;Juan G. Sánchez Novoa ,&nbsp;María D. Groppa ,&nbsp;Juana M. Navarro Llorens ,&nbsp;Patricia L. Marconi","doi":"10.1016/j.ram.2024.05.005","DOIUrl":"10.1016/j.ram.2024.05.005","url":null,"abstract":"<div><p>Cildáñez stream (in Matanza-Riachuelo basin, Buenos Aires) is one of the most polluted watercourses of Argentina, containing a mixed contamination from agricultural and industrial wastes. The application of water bioremediation processes for this kind of effluent will require microorganisms with a high tolerance to contamination. In this sense, obtaining higher contaminant-resistant microalgae lines is widely desired. In this study, adaptive laboratory evolution (ALE) and random mutagenesis were used to obtain <em>Chlorella vulgaris</em> LMPA-40 strains adapted to grow in polluted water from the Cildáñez stream. The ALE process was performed by 22 successive subcultures under selective pressure (Cildáñez wastewater alone or with the addition of phenol or H₂O₂) while random mutagenesis was performed with UV-C radiation at 275<!--> <!-->nm. Not all the cell lines obtained after ALE could adapt enough to overcome the stress caused by the Cildáñez wastewater, indicating that the process is quite random and depends on the stressor used. The best results were obtained for the Cildáñez wastewater adapted cells (Cild 3 strain) that were more resistant than the original strain. The concentration of protein, Chlorophyll A, Chlorophyll B, and carotenoids in the Cild 3 ALE evolved strain was higher than that of the control strain. However, this strain exhibited half of the lipid content compared to the same control strain. Interestingly, these alterations and the acquired tolerance may be reversed over time during storage. These findings suggest that the acquisition of novel cell lines could not be permanent, a fact that must be considered for future trials.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 241-248"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000816/pdfft?md5=7d5205e469aa55049c1fcd77cc9f95be&pid=1-s2.0-S0325754124000816-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primer caso autóctono de fiebre manchada asociado a la Reserva Ecológica Costanera Sur, Ciudad Autónoma de Buenos Aires","authors":"Jessica Monroig ,&nbsp;Marina de Seta ,&nbsp;Jose Carranza ,&nbsp;Sergio Giamperetti ,&nbsp;Bettina Deodato ,&nbsp;Rita Armitano ,&nbsp;Paula Díaz Pérez ,&nbsp;María Nazarena de Salvo ,&nbsp;Gabriel Leonardo Cicuttin ,&nbsp;Susana Lloveras","doi":"10.1016/j.ram.2024.06.001","DOIUrl":"10.1016/j.ram.2024.06.001","url":null,"abstract":"<div><p>The first autochthonous case of rickettsiosis is reported here. The case occurred in the Costanera Sur Ecological Reserve, a protected area of the City of Buenos Aires, in August 2022, where 4 species of ticks were found, namely <em>Amblyomma aureolatum</em>, <em>Ixodes auritulus sensu lato</em>, <em>Rhipicephalus sanguineus sensu stricto</em> and <em>Amblyomma triste.</em></p><p>The epidemiological, ecological, clinical and laboratory aspects that allowed timely diagnosis and appropriate treatment are also described.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 287-291"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000828/pdfft?md5=00f0f581658022972e07d5d945bf6286&pid=1-s2.0-S0325754124000828-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyanobacteria in soils under dryland agricultural production systems","authors":"","doi":"10.1016/j.ram.2024.03.003","DOIUrl":"10.1016/j.ram.2024.03.003","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 346-347"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000439/pdfft?md5=4dfffc4981b16b4081d100a7246a305c&pid=1-s2.0-S0325754124000439-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arthrobacter sp. UMCV2, and its compound N,N-dimethylhexadecilamine promote nodulation in Medicago truncatula by Sinorhizobium medicae","authors":"","doi":"10.1016/j.ram.2024.03.004","DOIUrl":"10.1016/j.ram.2024.03.004","url":null,"abstract":"<div><p>The actinobacterium <em>Arthrobacter</em> sp. UMCV2 promotes plant growth through the emission of <em>N</em>,<em>N</em>-dimethylhexadecilamine (DMHDA). The <em>Medicago</em>–<em>Sinorhizobium</em> nodulation has been employed to study symbiotic nitrogen fixation by rhizobia in nodulating Fabaceae. Herein, we isolated three <em>Sinorhizobium medicae</em> strains that were used to induce nodules in <em>Medicago truncatula</em>. The co-inoculation of <em>M. truncatula</em> with <em>Arthrobacter</em> sp. strain UMCV2 produced a higher number of effective nodules than inoculation with only <em>Sinorhizobium</em> strains. Similarly, the exposure of inoculated <em>M. truncatula</em> to DMHDA produced a greater number of effective nodules compared to non-exposed plants. Thus, we conclude that <em>Arthrobacter</em> sp. UMCV2 promotes nodulation, and propose that this effect is produced, at least partly, <em>via</em> DMHDA emission.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 292-297"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000440/pdfft?md5=84f0ab65fec625828e1d01d64c6ddff5&pid=1-s2.0-S0325754124000440-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141176249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are Mycoplasma pneumoniae coinfections frequent in COVID-19 patients? A systematic review","authors":"","doi":"10.1016/j.ram.2024.05.002","DOIUrl":"10.1016/j.ram.2024.05.002","url":null,"abstract":"<div><p>Understanding the proportion of SARS-CoV-2 patients with <em>Mycoplasma</em> <em>pneumoniae</em> coinfection is crucial for treating patients suffering from coronavirus disease (COVID-19), help to ensure responsible use of antibiotics and minimize the negative consequences of overuse. In addition, this knowledge could have an impact on empirical antibiotic management guidelines for patients with COVID-19. This systematic review aimed to identify the prevalence of <em>M. pneumoniae</em> in patients with coronavirus disease 2019 (COVID-19).</p><p>A bibliographic search of studies published in Spanish or English was conducted using the PubMed search engine. Fourteen articles from different continents (America, Asia and Europe) were included, involving a total of 5855 patients in these studies. The mean age of COVID-19 patients with <em>M. pneumoniae</em> was 48 years old (range 1–107), most of whom were male. The detection of laboratory-confirmed <em>M. pneumoniae</em> infection varied between 0 and 33.3%. Most of patients referred fever, cough, and dyspnea, and received empirical antibiotic treatment. Bacterial coinfection was not associated with increased ICU admission and mortality. The prevalence of coinfection showed extremely dissimilar figures according to the population studied and diagnostic criteria. However, it is important to develop Latin American studies, given the heterogeneity observed in the studies conducted in different countries. Standardized definitions should be developed in order to be able to assess the impact of coinfections in patients with a diagnosis of COVID-19.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 258-264"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000579/pdfft?md5=3b034561413e71059901f11d0e326c22&pid=1-s2.0-S0325754124000579-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors and mortality of candidemia in a children's public hospital in Sao Paulo, Brazil","authors":"","doi":"10.1016/j.ram.2023.09.002","DOIUrl":"10.1016/j.ram.2023.09.002","url":null,"abstract":"<div><p><em>Candida</em> bloodstream infections in children are of special concern in neonatal and pediatric intensive care and patients with comorbidities. This study aimed to estimate the incidence and risk factors associated with mortality in candidemia cases occurring in a public children's hospital in Ribeirao Preto, Brazil. It is a retrospective transversal study. Every patient under the age of 18 admitted to the study facility from January 1, 2013, to December 31, 2019, was considered potentially eligible to be included if they had candidemia. We collected clinical data from medical records. We included 113 blood cultures yielding positive results for <em>Candida</em>. The incidence rate was 2.12 per 1000 admissions. The most common <em>Candida</em> species was <em>Candida parapsilosis</em>. Septic shock during the candidemia episode was the only clinical outcome associated with a relative risk-adjusted (RRa) of 2.77 with an interval &gt;1 (1.12–6.85). Our findings show that the incidence rate and mortality rates of candidemia are in line with those in other children's services in Brazil. We found a global mortality rate of 28.31% (32/113) from candidemia episodes. We highlight the predominance of non-albicans <em>Candida</em> species including <em>C. parapsilosis</em>. Septic shock was the most important factor showing a significant risk of mortality.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 281-286"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000397/pdfft?md5=6754201fe6750d6241830260f8b16981&pid=1-s2.0-S0325754124000397-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140757970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of monkeypox virus in central Argentina: Epidemiological features and first complete genome sequences in the country","authors":"Gonzalo M. Castro ,&nbsp;Paola E. Sicilia ,&nbsp;Ana Willington ,&nbsp;Laura López ,&nbsp;Tomás Poklepovich ,&nbsp;Josefina Campos ,&nbsp;María G. Barbás","doi":"10.1016/j.ram.2024.05.003","DOIUrl":"10.1016/j.ram.2024.05.003","url":null,"abstract":"<div><p>Monkeypox (Mpox) is a zoonotic disease caused by the monkeypox virus (MPXV). MPXV can be transmitted by close contact with lesions, body fluids, respiratory droplets, and contaminated materials. A new pattern of spread among sexual networks has been recently described. The present work aimed to report the epidemiological and genomic characterization of the 2022 MPXV outbreak in central Argentina. A total of 113 scabs and/or lesion swab specimens were studied. MPXV infection was confirmed in 46.0% of the studied patients, all of whom were men. Varicella-zoster virus infection was the most frequent differential diagnosis. Eight complete viral genomes were obtained by next-generation sequencing. The Argentinian sequences were grouped intermingled with other sequences from the 2022 MPXV outbreak, related to samples from the USA, Europe, and Peru. Taken together, our study provided an initial assessment of the genetic and epidemiological characteristics of the 2022 MPXV outbreak in Córdoba, Argentina.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 276-280"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000774/pdfft?md5=19901670a2a3199969206463fb17a33b&pid=1-s2.0-S0325754124000774-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a mixed additive based on Saccharomyces cerevisiae and Lactobacillus rhamnosus on broilers exposed to aflatoxin B1 by contaminated feed","authors":"Analía Silvia Fochesato ,&nbsp;María Pia Martínez ,&nbsp;Débora Cuello ,&nbsp;Valeria Lorena Poloni ,&nbsp;María Julieta Luna ,&nbsp;Alejandra Paola Magnoli ,&nbsp;Cristian Fernández ,&nbsp;Lilia Renée Cavaglieri","doi":"10.1016/j.ram.2023.11.006","DOIUrl":"10.1016/j.ram.2023.11.006","url":null,"abstract":"<div><p>The objective of the present study was to explore the influence of dietary supplementation with a mixed additive (MA) containing a probiotic and anti-mycotoxin (<em>Saccharomyces cerevisiae</em> RC016 and <em>Lactobacillus rhamnosus</em> RC007) and its interaction on the performance and health (biochemistry and liver/intestine histopathology) of broilers fed diets contaminated with aflatoxin B₁ (AFB₁) at 506<!--> <!-->000<!--> <!-->±<!--> <!-->22.1<!--> <!-->ng/kg. The MA contained <em>S. cerevisiae</em> RC016 (1<!--> <!-->×<!--> <!-->10⁷ <!-->cells/g) and <em>L. rhamnosus</em> RC007 (1<!--> <!-->×<!--> <!-->10⁸ <!-->cells/g) in relation 1:1. A total of sixty-one-day-old Cobb broilers were randomly allocated into four treatment groups with three replicates of 5 birds each for a five-week-old feeding experiment. The experimental diet for each treatment (T) was formulated as follows: T1, a commercial diet (CD); T2, CD<!--> <!-->+<!--> <!-->AFB₁; T3, CD<!--> <!-->+<!--> <!-->0.1% MA; T4, CD<!--> <!-->+<!--> <!-->AFB₁ <!-->+<!--> <!-->0.1% MA. The MA improved (<em>p</em> <!-->&lt;<!--> <!-->0.01) production parameters (weight gain, conversion rate, and carcass yield) and reduced (<em>p</em> <!-->&lt;<!--> <!-->0.01) the toxic effect of AFB₁ on the relative weight of the livers. In addition, the macro and microscopic alterations of livers and the possible intestinal injury related to histological damage in the presence of mycotoxin were reduced. The use of probiotic MA based on <em>S. cerevisiae</em> RC016 and <em>L. rhamnosus</em> RC007 in animal feed provides greater protection against mycotoxin contamination and is safe for use as a supplement in animal feed, providing beneficial effects that improve animal health and productivity. This is of great importance at the economic level for the avian production system.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 312-321"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000804/pdfft?md5=68ff0cace867ee2cdecf71f3b49554e1&pid=1-s2.0-S0325754124000804-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compilation of regulations and procedures for monitoring filamentous fungi in hospital environments","authors":"","doi":"10.1016/j.ram.2024.02.001","DOIUrl":"10.1016/j.ram.2024.02.001","url":null,"abstract":"<div><p>Air and surfaces in the hospital environment are a potential source of exposure to filamentous fungi (FF) that could cause invasive fungal diseases (IFD) in severely immunocompromised patients. The prevalent FF in IFD are species from the genera <em>Aspergillus</em>, <em>Fusarium</em>, <em>Scedosporium</em>, and those within the order Mucorales. We have compiled regulations and described the procedures used in the clinical mycology laboratory to assess the presence of FF in areas at risk for the development of IFD. The infection control committees of each establishment implement hospital policies to regulate and control processes aimed at preventing infections. Fungal load monitoring is an important step in this process to validate air quality in order to ensure a clean and protected environment for severely immunocompromised patients.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 210-216"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000208/pdfft?md5=35bdd040cc85ecd9ce83ca327592b4d5&pid=1-s2.0-S0325754124000208-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140766529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}