Karina A. Gomes, Juan I. Degiuseppe, Juan A. Stupka
{"title":"Norovirus outbreaks in a nursery school in Buenos Aires, Argentina","authors":"Karina A. Gomes, Juan I. Degiuseppe, Juan A. Stupka","doi":"10.1016/j.ram.2024.07.001","DOIUrl":"10.1016/j.ram.2024.07.001","url":null,"abstract":"<div><div>Norovirus (NoV) is the leading cause of outbreaks of acute gastroenteritis worldwide. These are non-enveloped viruses that are classified into 10 genogroups, of which genogroup I (GI), II (GII), IV (GIV), VIII (GVIII), and IX (GIX) are the ones that infect humans. Two outbreaks (A and B) of acute gastroenteritis that occurred in a nursery school are described. The first outbreak (A) occurred in November 2018, and the second (B) in February 2020. The detection of viral and bacterial pathogens was performed to study both outbreaks. Additionally, an epidemiological investigation of the outbreaks was conducted. In the analyzed fecal and vomit samples from both children and adults in the nursery school, NoV GII.4 [P16] Sydney 2012 and NoV GI.3 [P13] were detected in outbreaks A and B, respectively. Since the study of acute gastroenteritis outbreaks is underestimated in Argentina, it is necessary to design prevention, study, and control protocols, as well as to improve the outbreak notification system in our country.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 373-379"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leticia Diana , Lucas Mastroianni , Virginia Diana , Rodrigo Puentes
{"title":"Streptococcus spp. isolated from bovine mastitis: Antimicrobial sensitivity studies and disagreement evaluation between routine phenotypic diagnosis and molecular identification","authors":"Leticia Diana , Lucas Mastroianni , Virginia Diana , Rodrigo Puentes","doi":"10.1016/j.ram.2024.07.006","DOIUrl":"10.1016/j.ram.2024.07.006","url":null,"abstract":"<div><div>Bovine mastitis poses a significant threat to global dairy production, resulting in substantial losses in milk production. <em>Streptococcus</em> bacteria, particularly <em>Streptococcus uberis</em>, <em>Streptococcus agalactiae</em>, and <em>Streptococcus dysgalactiae</em>, are commonly implicated in this condition. An accurate diagnosis is crucial for implementing effective treatment and minimizing its impact on production. This study examined 115 <em>Streptococcus</em> strains isolated from bovine mastitis cases in Uruguay using PCR for species identification. Additionally, the resistance to tetracycline, erythromycin, and penicillin was assessed in 81 of the bacterial strains under study. Significant disparities between phenotypic and genotypic detection were evident across all three species, with only 31% of strains identified phenotypically aligning with PCR results. Phenotypic prevalence indicated <em>S. dysgalactiae</em> as the most prevalent (44.35%), followed by <em>S. uberis</em> (24.34%) and <em>S. agalactiae</em> (6.09%). However, the genotypic identification revealed <em>S. uberis</em> as the most prevalent, followed by <em>S. dysgalactiae</em>, while <em>S. agalactiae</em> remained the least prevalent. The high sensitivity and speed of PCR suggest its potential routine implementation for diagnosing bovine mastitis caused by <em>Streptococcus</em> in any laboratory. Although, penicillin resistance was practically nonexistent, tetracycline and erythromycin exhibit higher resistance levels across all three species studied. In conclusion, the study underlines the importance of early diagnosis, highlights variations in bacterial prevalence, and proposes PCR as a valuable diagnostic tool for <em>Streptococcus</em> species responsible for bovine mastitis.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 351-358"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fábio Antônio Antonelo , Mariana Soares Rodrigues , Thiago Jean Kraus , Mayara Souza da Silva , Paula Fernandes Montanher
{"title":"Cutaneous infection in an immunocompromised domestic cat caused by Setosphaeria turcica (Luttr.) Leonard & Suggs","authors":"Fábio Antônio Antonelo , Mariana Soares Rodrigues , Thiago Jean Kraus , Mayara Souza da Silva , Paula Fernandes Montanher","doi":"10.1016/j.ram.2024.08.003","DOIUrl":"10.1016/j.ram.2024.08.003","url":null,"abstract":"<div><div>This is the first report of a cutaneous infection in an immunocompromised domestic cat caused by <em>Setosphaeria turcica</em>. The investigation encompasses an assessment of its virulence factors and susceptibility to antifungal drugs. The isolated strain originated from a domestic cat displaying cutaneous lesions that tested positive for feline leukemia virus (FeLV) infection. Identification procedures employed both microscopic and molecular techniques, with molecular identification relying on ITS DNA sequencing. Enzymatic assays targeting lipase, phospholipase, protease, and keratinase yielded negative results, suggesting the prevalence of alternative virulence mechanisms. Successful treatment of the infection was achieved with itraconazole, and susceptibility testing confirmed its sensitivity to azoles and polyene antifungal drugs.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 390-393"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chagas disease and immunosuppressive therapy: When therapeutic interventions can harm","authors":"Catalina D. Alba Soto , Silvia A. Repetto","doi":"10.1016/j.ram.2024.11.004","DOIUrl":"10.1016/j.ram.2024.11.004","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 349-350"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anaerobic digestates in agricultural soils: A systematic review of their effects on antibiotic resistance genes","authors":"Marco Allegrini , María Celina Zabaloy","doi":"10.1016/j.ram.2024.07.005","DOIUrl":"10.1016/j.ram.2024.07.005","url":null,"abstract":"<div><div>Tackling the dissemination of antibiotic resistance is one of the main global challenges. Manures from animal production are a recognized source of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) requiring appropriate treatment methods. One of the main approaches for manure treatment is anaerobic digestion (AD). Meta-analyses have demonstrated that AD can significantly reduce the load of ARGs. However, antibiotics, ARGs and MGEs still remain in the final product (digestate). A sustainable agricultural use of digestates under the One Health framework requires wide assessments of their effects in the soil resistome. The objective of this review was to present the state of the art of digestate effects on ARGs of agricultural soils, focusing exclusively on digestates from animal manures. A systematic review was conducted. The examination of the resulting literature indicated that although temporal decays are observed for a variety of ARGs in single-application and repeated-applications experiments, for certain ARGs the pre-treatment or control levels are not restored. However, the low number of studies and the heterogeneous experimental conditions preclude a clear understanding of the fate of ARGs in soil and their risk for agroecosystems. The inclusion of multiple MGEs and the assessment of the long-term influence of digestates on soil properties and microbial communities could be keystones for a better understanding of the risks associated with digestate-induced changes in the soil resistome.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 394-401"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ricardo Delgadillo-Valles , Dolores A. Marquez-Salazar , Donato A. Rechy-Iruretagoyena , Gerson N. Hernandez-Acevedo , Jonathan I. Arauz-Cabrera , Edwin Barrios-Villa
{"title":"Investigación del perfil de resistencia a betalactámicos en cepas clínicas de Pseudomonas aeruginosa en Mexicali, 2019-2021","authors":"Ricardo Delgadillo-Valles , Dolores A. Marquez-Salazar , Donato A. Rechy-Iruretagoyena , Gerson N. Hernandez-Acevedo , Jonathan I. Arauz-Cabrera , Edwin Barrios-Villa","doi":"10.1016/j.ram.2024.10.002","DOIUrl":"10.1016/j.ram.2024.10.002","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> is a Gram-negative bacillus capable of developing in humid environments and animal tissue. The interest in this bacterium lies in its ability to cause opportunistic diseases in patients with cystic fibrosis and healthcare-associated infections (HAIs). The objective of our study was to characterize the resistance profile of strains causing HAIs isolated in hospitals within our community, from January 2019 to December 2021. This descriptive, prospective, and cross-sectional study involved the isolation of strains from January 2019 to December 2021 at the Autonomous University of Baja California (UABC). The identification of the strains was carried out using Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry, and the detection of beta-lactam resistance was performed according to the criteria of the Clinical and Laboratory Standards Institute as stipulated in the CLSI M100-S27 document. A total of 649 samples were obtained from January 2019 to December 2021, including sputum (335 samples), urine (119 samples), and wounds (91 samples). Resistance to carbapenems was 38.94% for meropenem and 21.97% for imipenem. For cephalosporins, there was a 21.05% resistance rate for cefepime, 22.9% for ceftazidime, and 24.78% for ceftolozane-tazobactam. The prevalence of antimicrobial resistance has increased over time, which is attributable to both selective pressure and the evolution of the microorganisms themselves.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 368-372"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan I. Eizaguirre , Clara Bruzone , Ignacio Duhourq , Diego Libkind , Pablo S. Aguilar
{"title":"Incidencia de microorganismos deteriorantes de la calidad de cerveza en microcervecerías de Buenos Aires","authors":"Juan I. Eizaguirre , Clara Bruzone , Ignacio Duhourq , Diego Libkind , Pablo S. Aguilar","doi":"10.1016/j.ram.2024.09.006","DOIUrl":"10.1016/j.ram.2024.09.006","url":null,"abstract":"<div><div>Microbial contaminations pose a significant concern within the brewing industry, exerting negative effects on the organoleptic quality of the product and leading to substantial economic losses. The exponential proliferation of craft breweries in Argentina in recent years has heightened the demand for constant improvements in processes to ensure excellence in beer production. However, the stringency of microbiological quality controls remains a vulnerable area. This study assesses the prevalence of beer contaminants in samples from 10 breweries located in Buenos Aires City (CABA) and Greater Buenos Aires area (GBA). The results revealed the presence of microorganisms in 70% of the analyzed samples. Fifteen bacteria and 19 yeasts were successfully isolated, with bacteria belonging to the genera <em>Acetobacter</em> and <em>Staphylococcus</em>, and yeasts to the genera <em>Saccharomyces</em>, <em>Lodderomyces</em>, <em>Candida</em>, and <em>Pichia</em>. Accurately identifying these microorganisms provides producers with the necessary information for formulating action plans to improve cleaning and sanitization protocols in their facilities. This proactive approach not only has the potential to mitigate economic losses associated with microbial contamination but also contributes to maintaining and elevating quality standards in regional craft beer production.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 402-412"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prokaryotes versus Eukaryotes: Which is the host and which is the guest?","authors":"Guillermo Tellez-Isaias , Dante Javier Bueno","doi":"10.1016/j.ram.2024.09.001","DOIUrl":"10.1016/j.ram.2024.09.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 201-202"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S032575412400107X/pdfft?md5=be3fafe56368886eb45684ed3b307a5b&pid=1-s2.0-S032575412400107X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142238801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Caracterización de una comunidad microbiana aislada de colonias de abejas melíferas","authors":"","doi":"10.1016/j.ram.2024.01.001","DOIUrl":"10.1016/j.ram.2024.01.001","url":null,"abstract":"<div><p>The microbial communities within honey bee colonies contribute to the defense against pathogens. The goal of this study was to isolate, identify, and lyophilize lactic acid bacteria and bifidobacteria from the gut of nurse bees and bee bread in <em>Apis mellifera</em> colonies. Bacterial cultures from the intestinal content were conducted, and subsequently identified, sequenced, and lyophilized. Cross-antagonism among them was also assessed. Studies based on 16 S rRNA gene Sanger sequencing revealed that the MC3 strain had 100% identity with <em>Bifidobacterium choladohabitans</em>, the PP2B strain showed 99.16% similarity with <em>Enterococcus faecium,</em> while the PP1 strain exhibited 99.49% similarity with <em>Lacticaseibacillus</em> sp. and the PP1B strain showed 99.32% similarity with <em>Lacticaseibacillus</em> sp. There was no evidence of cross-antagonism among the strains, and the lyophilization process showed good stability and conservation. This is the first report of the isolation of <em>B. choladohabitans</em> from honey bee gut in Argentina, and also associates the presence of <em>E. faecium</em> with bee bread.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 265-269"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000245/pdfft?md5=6493003c0502128f9f6bb57f28eb42bb&pid=1-s2.0-S0325754124000245-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140958925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Los fructanos de agave promueven la formación de biopelícula in vitro en el consorcio probiótico Lactobacillus delbrueckii ssp. lactis, L. delbrueckii ssp. bulgaricus y Streptococcus thermophilus","authors":"","doi":"10.1016/j.ram.2024.02.002","DOIUrl":"10.1016/j.ram.2024.02.002","url":null,"abstract":"<div><p>In recent years the relationship between the intestinal microbiota, the host and chronic non-communicable diseases has brought interest into the study of its formation and maintenance in the host. Lactic acid bacteria (BAL) are Gram-positive bacteria with probiotic activity, which have been associated with many health benefits, such as decreased body fat mass and lower risk of type II diabetes mellitus. One of the main colonization mechanisms and bacteria survival strategies is the production of biofilms and the use of prebiotics as substrates to achieve a balance within intestinal microbiota. However, there is not enough evidence to demonstrate the biofilm formation in the presence of agave fructans (AF). This study aimed to evaluate <em>in vitro</em> the biofilm formation in a consortium of lactic acid bacteria: <em>Lactobacillus delbrueckii</em> ssp. <em>lactis</em>, <em>Lactobacillus delbrueckii</em> ssp<em>. bulgaricus</em> y <em>Streptococcus thermophilus</em> in the presence of AF at different concentrations: 0%, 0,1%, 4%, 8% y 16%. The addition of 0,1% of AF correlates with the best capacity for biofilm formation. The findings imply the possibility of modulating the biofilm formation of lactic acid bacteria with AF. These results can contribute positively to the host, by generating intestinal homeostasis, colonization resistance, stability to food digestion and chemical modifications of drugs and carry out beneficial functions to the health.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 322-328"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S032575412400021X/pdfft?md5=7219cc8e88340733c0eaa2a67d5aa82b&pid=1-s2.0-S032575412400021X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140787164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}