Juana Vega , Carlos H. Rodriguez , Ignacio Viscardi , Carlos Vay , Silvina Torres , Emilce Tabares , Angela Famiglietti , Marcela Nastro
{"title":"First nosocomial outbreak of SME-4-producing Serratia marcescens in South America","authors":"Juana Vega , Carlos H. Rodriguez , Ignacio Viscardi , Carlos Vay , Silvina Torres , Emilce Tabares , Angela Famiglietti , Marcela Nastro","doi":"10.1016/j.ram.2023.02.002","DOIUrl":"10.1016/j.ram.2023.02.002","url":null,"abstract":"<div><p>Carbapenemase-producing-<em>Serratia marcescens</em> isolates, although infrequent, are considered important nosocomial pathogens due to their intrinsic resistance to polymyxins, which limits therapeutic options. We describe a nosocomial outbreak of SME-4-producing <em>S. marcescens</em> in Buenos Aires city which, in our knowledge, represents the first one in South America.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9409047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"From the magic bullet to nanotechnology: nanoantimicrobials as therapeutics to fight multidrug-resistant microorganisms","authors":"María Gabriela Paraje","doi":"10.1016/j.ram.2023.08.001","DOIUrl":"10.1016/j.ram.2023.08.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41132602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Primera evidencia de actividad enzimática nitrilasa en Xylaria sp., y su relación con la biosíntesis de ácido indol-3-acético","authors":"Jorge Ricaño-Rodríguez , Celeste Ricaño-Rodríguez , Daniela Luis-Yong , Oswaldo Guzmán-López","doi":"10.1016/j.ram.2023.01.008","DOIUrl":"https://doi.org/10.1016/j.ram.2023.01.008","url":null,"abstract":"<div><p>Endophytic fungi inhabit plant tissues internally and asymptomatically, and many of them are involved in the synthesis of bioactive metabolites of antifungal and therapeutic nature, as well as other compounds of biotechnological importance including indole derivatives, among many others. Ecologically, they provide some benefits to plants including protection against phytopathogens and promotion of root growth. In this sense, <em>Xylaria</em> sp. is a cellulose-decomposing fungus with biotechnological potential. It is worth mentioning that indole-3-acetic acid (IAA) also plays an extremely important role in plant–micro-organism interactions, as it is essential for physiology and proper plant morphological development. It is known that nitrile-hydrolytic enzymes (nitrilases) are involved in the synthesis of plant indole compounds; however, relatively little information is available concerning the nature of these enzymes in the fungal kingdom. In view of the above, through a biochemical and molecular-genetic approach, it has been demonstrated for the first time that <em>Xylaria</em> sp. carries out nitrile-hydrolytic enzyme activity using nitrogen and carbon-rich compounds as substrate. The studied strain increased its relative gene expression levels and showed mycelial growth, both in the presence of chemical compounds such as cyanobenzene and KCN. Thus, the results of this work suggest that the micro-organism is capable of degrading complex nitrogenous molecules. On the other hand, through fungal biofertilization, it was observed that <em>Xylaria</em> sp. promotes the development of the root system of <em>Arabidopsis thaliana</em> seedlings, in addition to synthesizing IAA.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49825018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jimena Seitz , Claudina Vissio , Judith Bertone , Lucas Marques , Julián Parada , Alicia Carranza , Gabriel Di Cola , Roberto Ambrogi , Pablo Tamiozzo
{"title":"Ureaplasma diversum–Ureaplasma sp. alone or concomitantly with Mycoplasma hyopneumoniae in pig lungs with and without pneumonia: A descriptive exploratory study in abattoirs","authors":"Jimena Seitz , Claudina Vissio , Judith Bertone , Lucas Marques , Julián Parada , Alicia Carranza , Gabriel Di Cola , Roberto Ambrogi , Pablo Tamiozzo","doi":"10.1016/j.ram.2023.02.003","DOIUrl":"10.1016/j.ram.2023.02.003","url":null,"abstract":"<div><p>Occurrence of <em>Ureaplasma diversum</em> (<em>U. diversum</em>) has been associated with reproductive failures in cattle and detected in pigs with and without pneumonia. However, its role in the porcine respiratory disease complex (PRDC) is unclear. A cross-sectional study was conducted in abattoirs, inspecting 280 pig lungs from eight herds. All the lungs were inspected, processed and classified according to the histopathological analysis. Moreover, bronchoalveolar lavage (BAL) specimens were collected and processed by PCR for detection of <em>U. diversum</em> and <em>Mycoplasma hyopneumoniae</em> (<em>M. hyopneumoniae</em>). <em>Ureaplasma</em> sp.–<em>U. diversum</em> and <em>M. hyopneumoniae</em> were detected in 17.1% and 29.3% of the analyzed BAL specimens, respectively. The concomitant presence of both microorganisms was detected in 12.5% of the inspected lungs. Both agents were found in lungs with and without pneumonia. <em>M. hyopneumoniae</em> was detected in 31.8% of pig lungs with enzootic pneumonia-like lesions, while <em>Ureaplasma</em> sp.–<em>U. diversum</em> was detected in 27.5% of lungs with these lesions. This descriptive exploratory study provides information for future experimental and field-based studies to better define the pathogenic role of this organism within the PRDC.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9395189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enterotoxigenic profiles and submerged and interface biofilms in Bacillus cereus group isolates from foods","authors":"Itzel-Maralhi Cruz-Facundo , Roberto Adame-Gómez , Natividad Castro-Alarcón , Jeiry Toribio-Jiménez , Yaneth Castro-Coronel , María-Cristina Santiago-Dionisio , Marco-Antonio Leyva-Vázquez , David Tafolla-Venegas , Arturo Ramírez-Peralta","doi":"10.1016/j.ram.2023.01.007","DOIUrl":"10.1016/j.ram.2023.01.007","url":null,"abstract":"<div><p>Biofilm formation by <em>Bacillus cereus</em> strains is now recognized as a systematic contamination mechanism in foods; the aim of this study was to evaluate the production of submerged and interface biofilms in strains of <em>B. cereus</em> group in different materials, the effect of dextrose, motility, the presence of genes related to biofilms and the enterotoxigenic profile of the strains. We determine biofilm production by safranin assay, motility on semi-solid medium, toxin gene profiling and genes related to biofilm production by PCR in <em>B. cereus</em> group isolated from food. In this study, we observe strains used a higher production of biofilms in PVC; in the BHI broth, no submerged biofilms were found compared to phenol red broth and phenol red broth supplemented with dextrose; no strains with the <em>ces</em> gene were found, the enterotoxin profile was the most common the profile that includes genes for the three enterotoxins. We observed a different distribution of <em>tasA</em> and <em>sipW</em> with the origin of isolation of the strain, being more frequent in the strains isolated from eggshell. The production and type of biofilms are differential according to the type of material and culture medium used.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9245705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María del Pilar Núñez , Matias Butler , Leonardo Matias Majul , Luis Alberto Diorio , Laura Noemi Levin , Sonia Rosenfeldt
{"title":"Colonization of cigarette butts, a toxic waste, by white rot fungi","authors":"María del Pilar Núñez , Matias Butler , Leonardo Matias Majul , Luis Alberto Diorio , Laura Noemi Levin , Sonia Rosenfeldt","doi":"10.1016/j.ram.2023.01.003","DOIUrl":"10.1016/j.ram.2023.01.003","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9520131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ignacio Martín Santarelli , Diego Jorge Manzella , María Lucía Gallo Vaulet , Marcelo Rodríguez Fermepín , Yanina Crespo , Santiago Toledo Monaca , Martín Dobarro , Sofía Isabel Fernández
{"title":"Cycle threshold predicted mortality in a cohort of patients with hematologic malignancies infected with SARS-CoV-2","authors":"Ignacio Martín Santarelli , Diego Jorge Manzella , María Lucía Gallo Vaulet , Marcelo Rodríguez Fermepín , Yanina Crespo , Santiago Toledo Monaca , Martín Dobarro , Sofía Isabel Fernández","doi":"10.1016/j.ram.2023.03.002","DOIUrl":"10.1016/j.ram.2023.03.002","url":null,"abstract":"<div><p>When a SARS-CoV-2 RT-qPCR test is performed, it may determine an indirect measure of viral load called cycle threshold (Ct). Respiratory samples with Ct <25.0 cycles are considered to contain a high viral load. We aimed to determine whether SARS-CoV-2 Ct at diagnosis could predict mortality in patients with hematologic malignancies (lymphomas, leukemias, multiple myeloma) who contracted COVID-19. We included 35 adults with COVID-19 confirmed by RT-qPCR performed at diagnosis. We evaluated mortality due to COVID-19 rather than mortality due to the hematologic neoplasm or all-cause mortality. Twenty-seven (27) patients survived and 8 died. The global mean Ct was 22.8 cycles with a median of 21.7. Among the survivors, the mean Ct was 24.2, and the median Ct value was 22.9 cycles. In the deceased patients, the mean Ct was 18.0 and the median Ct value was 17.0 cycles. Using the Wilcoxon Rank Sum test, we found a significant difference (<em>p</em> <!-->=<!--> <!-->0.035). SARS-CoV-2 Ct measured in nasal swabs obtained at diagnosis from patients with hematologic malignancies may be used to predict mortality.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10130324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9498491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carla Álvarez , Marisa Almuzara , Claudia Tosello , Daniel Stecher , Carlos Vay , Claudia Barberis
{"title":"Actinomyces europaeus (Gleimia europaea) asociado con absceso cerebral: comunicación de tres casos","authors":"Carla Álvarez , Marisa Almuzara , Claudia Tosello , Daniel Stecher , Carlos Vay , Claudia Barberis","doi":"10.1016/j.ram.2022.07.003","DOIUrl":"https://doi.org/10.1016/j.ram.2022.07.003","url":null,"abstract":"<div><p>A brain abscess is a focal infection characterized by a collection of pus in the brain parenchyma. It is a life-threatening condition that should be diagnosed and treated as soon as possible. We report here three cases of patients with otogenic brain abscesses of polymicrobial origin that had in common the isolation of <em>Actinomyces europaeus</em>, which has not been previously described in this location. <em>A. europaeus</em> was identified by the conventional methodology, matrix-associated laser deionization-time of flight mass spectrometry (MALDI-TOF MS) and <em>16S rRNA</em> gene sequencing. Antibiotic susceptibility was evaluated by the epsilometric method, and all isolates showed sensitivity to penicillin, vancomycin and linezolid, whereas susceptibility to clindamycin and erythromycin was variable. MALDI-TOF MS identification allowed a quick and reliable species level identification in order to provide a rapid and effective response to avoid treatment delay that could lead to increased morbidity and even mortality.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49785040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Julia Elena García , Mónica Ruiz , Guillermo Andrés Maroniche , Cecilia Creus , Mariana Puente , Myriam Sara Zawoznik , María Daniela Groppa
{"title":"Inoculation with Azospirillum argentinense Az19 improves the yield of maize subjected to water deficit at key stages of plant development","authors":"Julia Elena García , Mónica Ruiz , Guillermo Andrés Maroniche , Cecilia Creus , Mariana Puente , Myriam Sara Zawoznik , María Daniela Groppa","doi":"10.1016/j.ram.2023.01.002","DOIUrl":"10.1016/j.ram.2023.01.002","url":null,"abstract":"<div><p>Water deficit constitutes a severe limitation to agricultural productivity. In the context of sustainable crop production, the potential of microbial biotechnology to increase plant drought tolerance and improve crop yields under adverse conditions is gaining relevance. This work aimed to compare the performance of <em>Azospirillum</em> <em>argentinense</em> strain Az19 to that of strain Az39, the most widely used for commercial inoculants, when inoculated in maize plants exposed to water deficit. For this purpose, greenhouse and field assays were conducted. In the greenhouse experiment, strain Az19 prevented the adverse effect of water deficit at V2 stage on maize growth. Moreover, the percentage of fertile plants and the ear weight decreased significantly under water deficits imposed at V2 and flowering in Az39-inoculated plants but not in Az19-inoculated plants. In the first field trial with the commercial maize hybrid DOW DS 515 PW, Az19-inoculated plants were those which better tolerated the water deficit imposed. In the second field trial, two maize genotypes with differential drought sensitivity (LP 29<!--> <!-->×<!--> <!-->LP 2542, sensitive; LP 882 (923)<!--> <!-->×<!--> <!-->LP 4703, tolerant) were tested. Higher tolerance to water deficit was detected in plants inoculated with <em>A. argentinense</em> Az19, with a noticeable effect on grain yield components in the sensitive genotype. Based on these results, we propose the use of <em>A. argentinense</em> Az19 for the formulation of more targeted <em>Azospirillum</em>-based inoculants, suitable for agroecological areas subjected to seasonal water deficits.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9120839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mónica E. Martínez , Sandra L. Grenón , Jessica Benítez , Celia Labán , Marta E. Mollerach , Martha H. von Specht
{"title":"Peritonitis primaria por neumococo en un hospital pediátrico de Argentina","authors":"Mónica E. Martínez , Sandra L. Grenón , Jessica Benítez , Celia Labán , Marta E. Mollerach , Martha H. von Specht","doi":"10.1016/j.ram.2023.01.001","DOIUrl":"https://doi.org/10.1016/j.ram.2023.01.001","url":null,"abstract":"<div><p>Pneumococcal peritonitis represents a small subset of patients suffering from invasive pneumococcal disease (IPD). We describe 5 cases of primary peritonitis documented in the pediatric hospital over 15 years (2005-2020) of IPD surveillance. The patients, 3<!--> <!-->girls and 2<!--> <!-->boys with a mean age of 5 years, experienced peritoneal signs and symptoms; 3<!--> <!-->of them suffered from nephrotic syndrome. Based on the local resistance profiles, all isolates were sensitive to beta-lactams, one strain showed resistance to cotrimoxazole and tetracycline while another strain, to cotrimoxazole only. Serotypes found in 4/5 strains (one was non-viable) were: 1, 19F, 15C and 23A. Children were treated with third-generation cephalosporins or ampicillin, gentamicin and metronidazole and all of them evolved favorably. Pneumococcal etiology should be included in the differential diagnosis of acute abdominal pain in children. Our study aims to contribute to the knowledge of this condition and to the local epidemiology of IPD.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49785039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}