Revista Argentina de microbiologia最新文献

{"title":"Bartonella spp. in different species of bats from Misiones (Argentina)","authors":"","doi":"10.1016/j.ram.2024.04.001","DOIUrl":"10.1016/j.ram.2024.04.001","url":null,"abstract":"<div><p>The aim of this study was to detect vector-borne pathogens (Anaplasmataceae family, <em>Rickettsia</em> genus, and <em>Bartonella</em> genus) in bats from Misiones (Argentina). Thirty-three specimens were captured over 8 days using mist nets. Twenty (60.6%) blood samples were positive (11/13 <em>Artibeus lituratus</em>, 4/10 <em>Desmodus rotundus</em>, 4/8 <em>Carollia perspicillata</em>, and 1/2 <em>Myotis nigricans</em>) by PCR for the <em>gltA</em> gene fragment of <em>Bartonella</em>. All samples were negative by PCR for the <em>Anaplasmataceae</em> family and <em>Rickettsia</em> genus.</p><p>The phylogenetic analysis showed seven <em>Bartonella</em> genotypes. The three genotypes obtained from <em>A. lituratus</em>, 2 from <em>C. perspicillata</em>, and 1 from <em>D. rotundus</em> were related to <em>Bartonella</em> spp. from New World bats, while the sequence obtained from <em>M. nigricans</em> was related to Old World bats.</p><p>We identified a considerable diversity of <em>Bartonella</em> genotypes in a small number of bats, thus further research is required to better understand the complex bat–pathogen interaction.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 227-231"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000452/pdfft?md5=d4d6bdb92e2b6e7dc7c4ee7c253ddfed&pid=1-s2.0-S0325754124000452-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141318178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detección de microorganismos y genes de resistencia en hemocultivos positivos mediante FilmArray® panel BCID2: experiencia en un hospital pediátrico","authors":"Marilina Kuzawka,&nbsp;Martín Cassanelli,&nbsp;Fabrina Capecce,&nbsp;Rosana Pereda","doi":"10.1016/j.ram.2023.07.007","DOIUrl":"10.1016/j.ram.2023.07.007","url":null,"abstract":"<div><p>The rapid identification of microorganisms that cause bacteremia and their possible resistance markers are extremely important for the timely initiation of effective antibiotic therapy.</p><p>The FilmArray® panel BCID2 (an automated rapid <em>multiplex</em> PCR assay) detects microorganisms and resistance genes from positive blood cultures within one hour. The aim of this study was to compare the results obtained from the FilmArray® Panel BCID2 and conventional culture in pediatric patients, as well as the reporting times of both methods. Sixty (60) FilmArray® results were included in the analysis. BCID2 showed high agreement with culture in the identification of microorganisms in monomicrobial bacteremias. However, in polymicrobial blood cultures, BCID2 detected a greater number of microorganisms compared to conventional culture, specifically,1 <em>Staphylococcus aureus</em>, 3 <em>Staphylococcus epidermidis</em>, 1 <em>Enterococcus faecium</em>, 2 <em>Klebsiella oxytoc</em>a, 1 <em>Acinetobacter calcoaceticus-baumannii</em> complex, 1 <em>Bacteroides fragilis</em> and 1 <em>Haemophilus influenzae</em>. Furthermore, 88.3% (95%<span>C</span>I<span>:</span> 78.7-94.8) of the FilmArray® results coincided with conventional culture, while in polymicrobial bacteremias, BCID2 detected a greater number of microorganisms with respect to conventional culture [70% (95%CI: 39.3-91.5)]. The agreement of resistance genes was good with a few exceptions (one ESBL was not detected by FilmArray® and one <em>S. aureus</em> strain was characterized as methicillin-resistant by BCID2 and methicillin-sensitive by culture). When comparing the time elapsing since the blood culture was reported as positive up to the results were obtained, BCID2 had a median of 2<!--> <!-->h 50<!--> <!-->min (IQR of 1 h 58<!--> <!-->min to 9<!--> <!-->h 27<!--> <!-->min) while the conventional culture had a median of 45<!--> <!-->h 20<!--> <!-->min (IQR of 24<!--> <!-->h 57<!--> <!-->min to 63<!--> <!-->h 50<!--> <!-->min).</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 249-257"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000786/pdfft?md5=27adedd3b1f845cddbdc1d92d35b565f&pid=1-s2.0-S0325754124000786-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141856316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"La deficiencia en el transporte de N-acetilglucosamina en Lysinibacillus sphaericus ASB13052 afecta el proceso de esporulación e incrementa la actividad hemolítica de su S-layer","authors":"Julián Tarsitano ,&nbsp;Sabrina Sol Bockor ,&nbsp;María Mercedes Palomino ,&nbsp;Joaquina Fina Martin ,&nbsp;Sandra Mónica Ruzal ,&nbsp;Mariana Claudia Allievi","doi":"10.1016/j.ram.2024.05.006","DOIUrl":"10.1016/j.ram.2024.05.006","url":null,"abstract":"<div><p><em>Lysinibacillus sphaericus</em> is a bacterium that, along with <em>Bacillus thuringiensis</em> var. <em>israelensis</em>, is considered the best biological insecticide for controlling mosquito larvae and an eco-friendly alternative to chemical insecticides. It depends on peptidic molecules such as N-acetylglucosamine to obtain carbon sources and possesses a phosphotransferase system (PTS) for their incorporation. Some strains carry S-layer proteins, whose involvement in metal retention and larvicidal activity against disease-carrying mosquitoes has been demonstrated. Alterations in the amino sugar incorporation system could affect the protein profile and functionality. Strain ASB13052 and the isogenic mutant in the <em>ptsH</em> gene, which is predominant in the PTS signaling pathway, were used in this study. For the first time, the presence of N-glycosylated S-layer proteins was confirmed in both strains, with a variation in their molecular weight pattern depending on the growth phase. In the exponential phase, an S-layer protein greater than 130 kDa was found in the <em>ptsH</em> mutant, which was absent in the wild-type strain. The mutant strain exhibited altered and incomplete low quality sporulation processes. Hemolysis analysis, associated with larvicidal activity, showed that the <em>ptsH</em> mutant has higher lytic efficiency, correlating with the high molecular weight protein. The results allow us to propose the potential effects that arise as a result of the absence of amino sugar transport on hemolytic activity, S-layer isoforms, and the role of N-acetylglucosamine in larvicidal activity.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 232-240"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S032575412400083X/pdfft?md5=7c35b9d4ac7b41a49f9e26ab51490125&pid=1-s2.0-S032575412400083X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142111472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution, seroprevalence and risk factors for bovine brucellosis in Brazil: Official data, systematic review and meta-analysis","authors":"Isis Daniele dos Santos Rocha ,&nbsp;Inácio José Clementino ,&nbsp;Débora Luise Canuto de Sousa ,&nbsp;Clebert José Alves ,&nbsp;Carolina de Sousa Américo Batista Santos ,&nbsp;Sérgio Santos de Azevedo","doi":"10.1016/j.ram.2023.08.002","DOIUrl":"10.1016/j.ram.2023.08.002","url":null,"abstract":"<div><p>Bovine brucellosis is an endemic disease in Brazil, and evidence-based assessments of the available literature on its seroprevalence and risk factors are limited. The aim of this study was to systematically review and summarize studies related to seroprevalence and risk factors of bovine brucellosis in the entire Brazil, in addition to comparing published data with the most recent official reports. Articles available in scientific databases and published between October 2006 and October 2021 were evaluated. Forty-five publications were included in the meta-analysis on the seroprevalence of brucellosis and 29 publications in the review on risk factors. The largest number of publications was found for the State of Mato Grosso do Sul (n<!--> <!-->=<!--> <!-->4), and the highest and lowest seroprevalences were observed in Acre (11%; 95% CI: 8.0–14.0%) and in the Federal District (0.4%; 95% CI: 0.2–0.7%). The main risk factors were the purchase of animals for breeding, vaccination, the number of heifers (female ≥2 years), the presence of calving paddocks and the occurrence of abortions. The need for new official studies has been suggested to determine the true prevalence of bovine brucellosis in Brazil, supported by the National Program for the Control and Eradication of Animal Brucellosis and Tuberculosis.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 153-164"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123001098/pdfft?md5=e16b23c9849da0007966bfe65aa14673&pid=1-s2.0-S0325754123001098-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139098560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional study of genes involved in the passage from teliospore to hyphae stage in the fungus Thecaphora frezii, the causal agent of peanut smut","authors":"María S. Díaz ,&nbsp;Néstor W. Soria ,&nbsp;Ana C. Figueroa ,&nbsp;Pablo Yang ,&nbsp;Esteban H. Badariotti ,&nbsp;Valeria R. Alasino ,&nbsp;Pablo Vélez ,&nbsp;Dante M. Beltramo","doi":"10.1016/j.ram.2023.10.002","DOIUrl":"10.1016/j.ram.2023.10.002","url":null,"abstract":"<div><p>Peanuts (<em>Arachis hypogaea</em> L.) are among the most important leguminous crops in Argentina. During the growing season, they are frequently attacked by fungal diseases, including <em>Thecaphora frezii</em>. The spores of <em>T. frezii</em> are structures that confer resistance to this phytopathogen. The transition from teliospore to hypha is a characteristic process of some fungi, which is essential for completing their life cycle. Using the transcriptomes of teliospores and hyphae of <em>T. frezii</em>, we aimed to identify genes that were differentially expressed during this transition, and we found 134 up-regulated and 66 down-regulated genes, which would participate in different cellular processes such as: (a) cell cycle and DNA processing; (b) cell fate; (c) rescue, defense and cellular virulence; (d) detoxification by CYP450; (e) energy; (f) nutrient interaction and nutritional adaptation; (g) metabolism; (g) proteins with binding functions or cofactor requirements; (h) stress, cell differentiation and biogenesis of cell components; and (i) transport, cell communication and transcription. The identification of genes in <em>T. frezii</em> and their expression levels during different stages of differentiation could contribute to our understanding of the biological mechanisms in this fungus.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 175-186"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000038/pdfft?md5=11d862ad8a5aea638a6c5f4342331d5a&pid=1-s2.0-S0325754124000038-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139712941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenemen-resistant Enterobacterales: An issue of global concern","authors":"Marcela Nastro","doi":"10.1016/j.ram.2024.05.001","DOIUrl":"https://doi.org/10.1016/j.ram.2024.05.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 113-114"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000543/pdfft?md5=3f3319840a603f1a595172449cfe944a&pid=1-s2.0-S0325754124000543-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141444232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalencia y distribución geográfica de las enfermedades de transmisión sexual de los bovinos en la provincia de Formosa, Argentina","authors":"María Nair Viola ,&nbsp;Iris Carolina Elías ,&nbsp;Marcelo Signorini ,&nbsp;Ana Ines Molineri ,&nbsp;Ana María Russo ,&nbsp;Patricia Andrea Zimmer ,&nbsp;Laura Analía Lozina ,&nbsp;Juana Noemí Gimenez","doi":"10.1016/j.ram.2023.07.004","DOIUrl":"10.1016/j.ram.2023.07.004","url":null,"abstract":"<div><p>Bovine genital campylobacteriosis (BGC) and bovine trichomonosis (BT) are sexually transmitted diseases (STDs) that affect bovine breeding herds, decreasing their reproductive efficiency. The objective of this work was to estimate the prevalence of these diseases and their temporal-spatial distribution in the province of Formosa, Argentina. The cross-sectional study conducted between 2018 and 2021 included a total of 15,571 bulls, inter-herd prevalence being 29.62% and 17.23% for BGC and BT, respectively. The prevalence of positive animals was 2.05% for BGC and 0.43% for BT. The temporal-spatial analysis of BGC showed two distinct spatial groupings, one group had a low risk of contracting the disease (RR = 0.13; <em>p</em> &lt; 0.001; 2018–2021) while the other group had a high risk (RR = 2.84; <em>p</em> &lt; 0.001; 2020–2021). BT had a high-risk group for the disease (RR = 35.24<em>; p</em> &lt; 0.001; 2019). This study shows that STDs are endemic in the region, providing updated and valuable information as a tool for the health management of these diseases.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 147-152"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000627/pdfft?md5=756acbfa3de90a2c6d1cbd1f7ba2b321&pid=1-s2.0-S0325754123000627-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72015171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The microPIXE technique to understand the distribution of heavy metals in arbuscular mycorrhizal symbiosis","authors":"M. Statello ,&nbsp;R.P. Colombo ,&nbsp;E.M. de la Fournière ,&nbsp;M.E. Debray ,&nbsp;A.M. Godeas ,&nbsp;V.A. Silvani","doi":"10.1016/j.ram.2023.12.006","DOIUrl":"10.1016/j.ram.2023.12.006","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 198-199"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000415/pdfft?md5=b3805e315e1a3f2fc92699fcde771a22&pid=1-s2.0-S0325754124000415-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140945783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carpogenic germination of Sclerotinia sclerotiorum sclerotia in Brassica oleracea var gemmifera (VN: Brussels sprouts) in North Patagonia, Argentina","authors":"A.G. Scarso ,&nbsp;M.C. Sosa ,&nbsp;M.J. Ousset ,&nbsp;M.C. Lutz","doi":"10.1016/j.ram.2023.12.005","DOIUrl":"10.1016/j.ram.2023.12.005","url":null,"abstract":"<div><p>In August 2018, symptoms of apical and basal rot resembling those caused by <em>Sclerotinia sclerotiorum</em> infection were observed in a commercial Brussels sprouts field in North Patagonia, Argentina. The incidence of apical and basal rot was 23.30% and 2.30%, respectively. Carpogenic germination of sclerotia was detected in shaded, highly humid soil areas. To our knowledge, this is the first report of carpogenic germination of sclerotia from S<em>. sclerotiorum</em> in North Patagonia.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 187-190"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000075/pdfft?md5=9c4bfde9b0cf122087818419cb0e383b&pid=1-s2.0-S0325754124000075-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140190067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In house-development of a rapid immunochromatographic test for the detection of Escherichia coli in urine samples","authors":"Paula Da Cunda ,&nbsp;Amy Mónaco ,&nbsp;María Moreno ,&nbsp;María José Gonzalez ,&nbsp;Paola Scavone ,&nbsp;Luciana Robino","doi":"10.1016/j.ram.2023.10.001","DOIUrl":"10.1016/j.ram.2023.10.001","url":null,"abstract":"<div><p>Urinary tract infections (UTIs) are a common health concern. Urine culture is the “gold standard” for UTI diagnosis but takes 48<!--> <!-->h. Rapid methods like dipstick tests are used as point-of-care tests. However, their sensitivity and specificity are variable. In this work, a rapid immunochromatographic test (IT) for detecting <em>Escherichia coli</em> in urine was developed, and its performance was evaluated in urine samples from patients with suspected UTI. The “universal lateral flow assay kit” was employed using an <em>E. coli</em> capture antibody. One hundred and five (105) urine samples were analyzed using the IT, dipstick test, and urine culture. The sensitivity of the IT was 74.5%, specificity 88.9%, positive predictive value (PPV) 86.3%, and negative predictive value (NPV) 78.7%. The combination of the IT with the dipstick test increases sensitivity to 94.1%, specificity to 66.7%, PPV to 72.7%, and NPV to 92.3%. Using the IT for detecting <em>E. coli</em> in urine could be a valuable technique for UTI screening, showing better specificity and diagnostic precision but lower sensitivity than the dipstick test. Based on these results, we propose that the combined use of both screening techniques would allow a rapid and more precise diagnosis of UTI, rationalizing the indication for empirical antibiotics.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 140-146"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000846/pdfft?md5=5dd878bb5bfa171fb4bec9b4b72511e8&pid=1-s2.0-S0325754123000846-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138558474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}