Revista Argentina de microbiologia最新文献

{"title":"Primer aislamiento de Acanthamoeba spp. en agua de mar del sudeste bonaerense, Argentina","authors":"","doi":"10.1016/j.ram.2024.02.006","DOIUrl":"10.1016/j.ram.2024.02.006","url":null,"abstract":"<div><p>Free-living amoebae (FLA) of the genus <em>Acanthamoeba</em> are ubiquitous and amphizoic protozoa that colonize aquatic and terrestrial habitats and can serve as reservoirs for other microorganisms. They are considered econoses that can cause severe and rare pathologies. Due to limited epidemiological data available, the objective of this study was to investigate the presence of <em>Acanthamoeba</em> in coastal wetlands of the southeast of Buenos Aires province and evaluate their association with bacteriological and environmental variables. From February 2021 to July 2022, 22 seawater samples were collected at different points along the coast of the city of Mar del Plata (Buenos Aires, Argentina). Environmental parameters were determined and physicochemical and bacteriological studies, morphological identification, cultures and molecular typification were conducted. Regardless of the environmental and bacteriological variables, the presence of <em>Acanthamoeba</em> spp. was molecularly confirmed in 54.54% of the samples, being the first report of these protozoa in seawater in Argentina.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 221-226"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000385/pdfft?md5=8a7ab49ad87cd955e7725eb083d57278&pid=1-s2.0-S0325754124000385-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140791714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of lineage III of Shigella sonnei ST152 belonging to a high-risk clone harboring the blaCTX-M-15 gene in Peru","authors":"","doi":"10.1016/j.ram.2024.02.007","DOIUrl":"10.1016/j.ram.2024.02.007","url":null,"abstract":"<div><p>Multidrug-resistant <em>Shigella sonnei</em> ST152, global lineage III, is a high-risk clone, whose dissemination has limited therapeutic options for shigellosis. This study aimed to characterize two isolates of <em>S. sonnei</em>, which were recovered in Lima, Peru, during November 2019, exhibiting resistance to extended-spectrum cephalosporins and quinolones, and concurrently harboring <em>bla</em>_CTX-M-15 and <em>qnrS1</em> genes, in addition to mutations in <em>gyrA</em>-S83L. These isolates were resistant to ceftriaxone, ciprofloxacin and trimethoprim/sulfamethoxazole. The molecular analysis showed that both isolates belonged to lineage III, sublineages IIIa and IIIb. The <em>bla</em>_CTX-M-15 gene was located in the same genetic platform as <em>qnrS1</em>, flanked upstream by ISKpn19, on a conjugative plasmid belonging to the IncI-γ group. To the best of our knowledge, this would be the first report on <em>S. sonnei</em> isolates carrying the <em>bla</em>_CTX-M-15 gene in Peru. The global dissemination of <em>S. sonnei</em> ST152, co-resistant to β-lactams and quinolones, could lead to a worrisome scenario in the event of potential acquisition of genetic resistance mechanisms to azithromycin.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 205-209"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000403/pdfft?md5=6c80b341bbe0d9cd0ab43a7c2bf50852&pid=1-s2.0-S0325754124000403-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identificación y actividad antifúngica de bacterias halófilas aisladas de suelos salinos en Campeche, México","authors":"","doi":"10.1016/j.ram.2024.02.004","DOIUrl":"10.1016/j.ram.2024.02.004","url":null,"abstract":"<div><p>Phytopathogenic fungi <em>Alternaria alternata</em> and <em>Colletotrichum gloeosporioide</em>s cause diseases in plant tissues as well as significant postharvest losses. The use of chemical fungicides for their control has negative effects on health and the environment. Secondary metabolites from halophilic bacteria are a promising alternative for new antifungal compounds. In the present study, halophilic bacteria were isolated and characterized from two sites with saline soils called branquizales in Campeche, Mexico. A total of 64 bacteria were isolated. <em>Agrobacterium</em>, <em>Bacillus</em>, <em>Inquilinus</em>, <em>Gracilibacillus</em>, <em>Metabacillus</em>, <em>Neobacillus</em>, <em>Paenibacillus</em>, <em>Priestia</em>, <em>Staphylococcus</em>, <em>Streptomyces</em> and <em>Virgibacillus</em> were among the identified genera. The antifungal potential of the culture supernatant (CS) of 39 halophilic bacteria was investigated against <em>C</em>. <em>gloeosporioides</em> and <em>A</em>. <em>alternata</em>. The bacteria showing the greatest inhibition of mycelial growth corresponded to <em>Bacillus subtilis</em> CPO 4292, <em>Metabacillus</em> sp. CPO 4266, <em>Bacillus</em> sp. CPO 4295 and <em>Bacillus</em> sp. CPO 4279. The CS of <em>Bacillus</em> sp. CPO 4279 exhibited the highest activity and its ethyl acetate extract (AcOEt) inhibited the germination of <em>C</em>. <em>gloeosporioides</em>, with IC₅₀ values of 8,630<!--> <!-->μg/ml and IC₉₀ of 10,720<!--> <!-->μg/ml. The organic partition of the AcOEt extract led to three fractions, with acetonitrile (FAcB9) showing the highest antifungal activity, with values exceeding 66%. Halophilic bacteria from ‘blanquizales’ soils of the genus <em>Bacillus</em> sp. produce metabolites with antifungal properties that inhibit the phytopathogenic fungus <em>C</em>. <em>gloeosporioides</em>.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 298-311"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000233/pdfft?md5=9262279ae0a4f9cff2fa928452c96f9c&pid=1-s2.0-S0325754124000233-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140788291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of vaccination with Brucella melitensis, strains Rev 1 ΔeryCD and Rev 1, on the reproductive system of young male goats","authors":"","doi":"10.1016/j.ram.2023.11.005","DOIUrl":"10.1016/j.ram.2023.11.005","url":null,"abstract":"<div><p>The present study evaluates the effects of vaccination with <em>Brucella melitensis</em> strains Rev 1 <em>ΔeryCD</em> and Rev 1 on the reproductive system of male goats. Three groups, each of them consisting of 15 six-month-old brucellosis-free male goats, were studied. The first group was vaccinated with the Rev 1 <em>ΔeryCD</em> strain, the second group received Rev 1 and the third group was inoculated with sterile physiological saline solution. The dose of both strains was of 1<!--> <!-->×<!--> <!-->10⁹ <!-->CFU/ml. Over the course of the five months of this study, three males from each group were euthanized every month. Their reproductive tracts, spleens, and lymph nodes were collected to analyze serology, bacteriology PCR, histology, and immunohistochemistry. Results show that vaccination with <em>B. melitensis</em> strains Rev 1 ΔeryCD and Rev 1 does not harm the reproductive system of male goats. Strain <em>B. melitensis</em> Rev 1 <em>ΔeryCD</em> displayed a lower capacity to colonize the reproductive tract than strain Rev 1, which was attributed to its limited catabolic action toward erythritol.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 270-275"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000051/pdfft?md5=adfb14f9a36ad3c95ab9bd88af08083b&pid=1-s2.0-S0325754124000051-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140055899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabiotics: do we need a new definition?","authors":"Gabriel Vinderola ,&nbsp;Marcela Carina Audisio","doi":"10.1016/j.ram.2024.09.002","DOIUrl":"10.1016/j.ram.2024.09.002","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 203-204"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124001081/pdfft?md5=6a11b3b1aa76572fb8cf890bcd0d81b2&pid=1-s2.0-S0325754124001081-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142238802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsporum canis pseudomycetoma and disseminated Mycobacterium genavense infection in an HIV/AIDS patient, an unusual combination","authors":"","doi":"10.1016/j.ram.2024.02.005","DOIUrl":"10.1016/j.ram.2024.02.005","url":null,"abstract":"<div><p>Pseudomycetomas are rare fungal subcutaneous infections caused by dermatophytes, which are mainly observed in immunocompromised patients. <em>Mycobacterium genavense</em> is considered an opportunistic pathogen in people living with HIV/AIDS (PLWHA), clinically resembling the presentation of <em>Mycobacterium avium</em> complex (MAC). Here, we describe the case of a 26-year-old PLWHA with a 3-month history of a 4<!--> <!-->cm tumoral, duroelastic and painful lesion located on the back. Histopathology of the tumoral lesion revealed chronic granulomatous inflammation with grains composed of PAS-positive and Grocott-positive septate hyphae, as well as acid-fast bacilli (AFB). Culture on Sabouraud and lactrimel agar developed colonies that were later identified as <em>Microsporum canis</em>. In successive samples, the AFB were identified as <em>M. genavense</em> by restriction analysis of PCR products. Immunocompromised PLWHA not only suffer increased susceptibility to diseases due to unusual pathogens but also atypical clinical presentation of frequently encountered pathogens.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 217-220"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000373/pdfft?md5=3e3d27183b4c9a9e346534784754f925&pid=1-s2.0-S0325754124000373-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140781874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bartonella spp. in different species of bats from Misiones (Argentina)","authors":"","doi":"10.1016/j.ram.2024.04.001","DOIUrl":"10.1016/j.ram.2024.04.001","url":null,"abstract":"<div><p>The aim of this study was to detect vector-borne pathogens (Anaplasmataceae family, <em>Rickettsia</em> genus, and <em>Bartonella</em> genus) in bats from Misiones (Argentina). Thirty-three specimens were captured over 8 days using mist nets. Twenty (60.6%) blood samples were positive (11/13 <em>Artibeus lituratus</em>, 4/10 <em>Desmodus rotundus</em>, 4/8 <em>Carollia perspicillata</em>, and 1/2 <em>Myotis nigricans</em>) by PCR for the <em>gltA</em> gene fragment of <em>Bartonella</em>. All samples were negative by PCR for the <em>Anaplasmataceae</em> family and <em>Rickettsia</em> genus.</p><p>The phylogenetic analysis showed seven <em>Bartonella</em> genotypes. The three genotypes obtained from <em>A. lituratus</em>, 2 from <em>C. perspicillata</em>, and 1 from <em>D. rotundus</em> were related to <em>Bartonella</em> spp. from New World bats, while the sequence obtained from <em>M. nigricans</em> was related to Old World bats.</p><p>We identified a considerable diversity of <em>Bartonella</em> genotypes in a small number of bats, thus further research is required to better understand the complex bat–pathogen interaction.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 227-231"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000452/pdfft?md5=d4d6bdb92e2b6e7dc7c4ee7c253ddfed&pid=1-s2.0-S0325754124000452-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141318178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detección de microorganismos y genes de resistencia en hemocultivos positivos mediante FilmArray® panel BCID2: experiencia en un hospital pediátrico","authors":"Marilina Kuzawka,&nbsp;Martín Cassanelli,&nbsp;Fabrina Capecce,&nbsp;Rosana Pereda","doi":"10.1016/j.ram.2023.07.007","DOIUrl":"10.1016/j.ram.2023.07.007","url":null,"abstract":"<div><p>The rapid identification of microorganisms that cause bacteremia and their possible resistance markers are extremely important for the timely initiation of effective antibiotic therapy.</p><p>The FilmArray® panel BCID2 (an automated rapid <em>multiplex</em> PCR assay) detects microorganisms and resistance genes from positive blood cultures within one hour. The aim of this study was to compare the results obtained from the FilmArray® Panel BCID2 and conventional culture in pediatric patients, as well as the reporting times of both methods. Sixty (60) FilmArray® results were included in the analysis. BCID2 showed high agreement with culture in the identification of microorganisms in monomicrobial bacteremias. However, in polymicrobial blood cultures, BCID2 detected a greater number of microorganisms compared to conventional culture, specifically,1 <em>Staphylococcus aureus</em>, 3 <em>Staphylococcus epidermidis</em>, 1 <em>Enterococcus faecium</em>, 2 <em>Klebsiella oxytoc</em>a, 1 <em>Acinetobacter calcoaceticus-baumannii</em> complex, 1 <em>Bacteroides fragilis</em> and 1 <em>Haemophilus influenzae</em>. Furthermore, 88.3% (95%<span>C</span>I<span>:</span> 78.7-94.8) of the FilmArray® results coincided with conventional culture, while in polymicrobial bacteremias, BCID2 detected a greater number of microorganisms with respect to conventional culture [70% (95%CI: 39.3-91.5)]. The agreement of resistance genes was good with a few exceptions (one ESBL was not detected by FilmArray® and one <em>S. aureus</em> strain was characterized as methicillin-resistant by BCID2 and methicillin-sensitive by culture). When comparing the time elapsing since the blood culture was reported as positive up to the results were obtained, BCID2 had a median of 2<!--> <!-->h 50<!--> <!-->min (IQR of 1 h 58<!--> <!-->min to 9<!--> <!-->h 27<!--> <!-->min) while the conventional culture had a median of 45<!--> <!-->h 20<!--> <!-->min (IQR of 24<!--> <!-->h 57<!--> <!-->min to 63<!--> <!-->h 50<!--> <!-->min).</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 249-257"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000786/pdfft?md5=27adedd3b1f845cddbdc1d92d35b565f&pid=1-s2.0-S0325754124000786-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141856316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"La deficiencia en el transporte de N-acetilglucosamina en Lysinibacillus sphaericus ASB13052 afecta el proceso de esporulación e incrementa la actividad hemolítica de su S-layer","authors":"Julián Tarsitano ,&nbsp;Sabrina Sol Bockor ,&nbsp;María Mercedes Palomino ,&nbsp;Joaquina Fina Martin ,&nbsp;Sandra Mónica Ruzal ,&nbsp;Mariana Claudia Allievi","doi":"10.1016/j.ram.2024.05.006","DOIUrl":"10.1016/j.ram.2024.05.006","url":null,"abstract":"<div><p><em>Lysinibacillus sphaericus</em> is a bacterium that, along with <em>Bacillus thuringiensis</em> var. <em>israelensis</em>, is considered the best biological insecticide for controlling mosquito larvae and an eco-friendly alternative to chemical insecticides. It depends on peptidic molecules such as N-acetylglucosamine to obtain carbon sources and possesses a phosphotransferase system (PTS) for their incorporation. Some strains carry S-layer proteins, whose involvement in metal retention and larvicidal activity against disease-carrying mosquitoes has been demonstrated. Alterations in the amino sugar incorporation system could affect the protein profile and functionality. Strain ASB13052 and the isogenic mutant in the <em>ptsH</em> gene, which is predominant in the PTS signaling pathway, were used in this study. For the first time, the presence of N-glycosylated S-layer proteins was confirmed in both strains, with a variation in their molecular weight pattern depending on the growth phase. In the exponential phase, an S-layer protein greater than 130 kDa was found in the <em>ptsH</em> mutant, which was absent in the wild-type strain. The mutant strain exhibited altered and incomplete low quality sporulation processes. Hemolysis analysis, associated with larvicidal activity, showed that the <em>ptsH</em> mutant has higher lytic efficiency, correlating with the high molecular weight protein. The results allow us to propose the potential effects that arise as a result of the absence of amino sugar transport on hemolytic activity, S-layer isoforms, and the role of N-acetylglucosamine in larvicidal activity.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 232-240"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S032575412400083X/pdfft?md5=7c35b9d4ac7b41a49f9e26ab51490125&pid=1-s2.0-S032575412400083X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142111472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution, seroprevalence and risk factors for bovine brucellosis in Brazil: Official data, systematic review and meta-analysis","authors":"Isis Daniele dos Santos Rocha ,&nbsp;Inácio José Clementino ,&nbsp;Débora Luise Canuto de Sousa ,&nbsp;Clebert José Alves ,&nbsp;Carolina de Sousa Américo Batista Santos ,&nbsp;Sérgio Santos de Azevedo","doi":"10.1016/j.ram.2023.08.002","DOIUrl":"10.1016/j.ram.2023.08.002","url":null,"abstract":"<div><p>Bovine brucellosis is an endemic disease in Brazil, and evidence-based assessments of the available literature on its seroprevalence and risk factors are limited. The aim of this study was to systematically review and summarize studies related to seroprevalence and risk factors of bovine brucellosis in the entire Brazil, in addition to comparing published data with the most recent official reports. Articles available in scientific databases and published between October 2006 and October 2021 were evaluated. Forty-five publications were included in the meta-analysis on the seroprevalence of brucellosis and 29 publications in the review on risk factors. The largest number of publications was found for the State of Mato Grosso do Sul (n<!--> <!-->=<!--> <!-->4), and the highest and lowest seroprevalences were observed in Acre (11%; 95% CI: 8.0–14.0%) and in the Federal District (0.4%; 95% CI: 0.2–0.7%). The main risk factors were the purchase of animals for breeding, vaccination, the number of heifers (female ≥2 years), the presence of calving paddocks and the occurrence of abortions. The need for new official studies has been suggested to determine the true prevalence of bovine brucellosis in Brazil, supported by the National Program for the Control and Eradication of Animal Brucellosis and Tuberculosis.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 2","pages":"Pages 153-164"},"PeriodicalIF":1.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123001098/pdfft?md5=e16b23c9849da0007966bfe65aa14673&pid=1-s2.0-S0325754123001098-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139098560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}