Caracterización molecular de aislamientos de Listeria monocytogenes de origen humano y alimentario en Argentina, 2018-2023

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-01 DOI:10.1016/j.ram.2024.03.002

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引用次数: 0

Abstract

Human listeriosis is an infectious disease caused by Listeria monocytogenes. The invasive form of this disease leads to a high rate of hospitalizations and fatality. The main mode of transmission is through contaminated ready-to-eat foods such as dairy, vegetables and meat products. The knowledge of the diversity and population dynamics of isolates collected from human and food sources is essential for the detection of clusters and the identification of common sites of infection. The aim of this study was the molecular characterization of L. monocytogenes isolates in Argentina. We sequenced a total of 63 isolates, 35 from human and 28 from food sources, collected between 2018 and 2023. Our genomic study divided the isolates into two lineages, four serogroups, 17 sequence types and 15 clonal complexes (CCs). The hypervirulent clone CC1 (lineage I; serogroup IVb) predominated in human and food samples. The phylogenomic analysis showed a high and possible epidemiological relationship between isolates from human and/or food sources, suggesting the presence of transmission chains in our country. These findings highlight the need to strengthen genomic surveillance of L. monocytogenes in Argentina. The identification of geographic distribution and characteristics of predominant and emerging clones from human and food sources might help to focus action plans and public health policies better directed at the control and prevention of listeriosis.

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[2018-2023年阿根廷从人类和食物中分离的单核细胞增生李斯特菌的分子特征]。

人类李斯特菌病是由单核细胞增生李斯特菌引起的一种传染病。这种疾病的侵袭性形式导致很高的住院率和死亡率。主要传播方式是通过受污染的即食食品，如乳制品、蔬菜和肉制品。了解从人类和食物中收集的分离物的多样性和种群动态，对于检测集群和确定共同的感染地点至关重要。本研究的目的是对阿根廷的单核细胞增生性酵母菌分离物进行分子鉴定。我们对 2018 年至 2023 年间收集的 63 个分离物进行了测序，其中 35 个来自人类，28 个来自食物来源。我们的基因组研究将分离物分为两个系、四个血清群、17 个序列类型和 15 个克隆复合体（CC）。在人类和食物样本中，高病毒性克隆CC1（系I；血清群IVb）占主导地位。系统发生组分析表明，来自人类和/或食物来源的分离物之间存在高度和可能的流行病学关系，表明我国存在传播链。这些发现凸显了加强阿根廷单核细胞增多症基因组监测的必要性。确定来自人类和食物来源的主要克隆和新出现克隆的地理分布和特征，可能有助于使行动计划和公共卫生政策更有针对性，从而更好地控制和预防李斯特菌病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.