Effects of vaccination with Brucella melitensis, strains Rev 1 ΔeryCD and Rev 1, on the reproductive system of young male goats

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
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引用次数: 0

Abstract

The present study evaluates the effects of vaccination with Brucella melitensis strains Rev 1 ΔeryCD and Rev 1 on the reproductive system of male goats. Three groups, each of them consisting of 15 six-month-old brucellosis-free male goats, were studied. The first group was vaccinated with the Rev 1 ΔeryCD strain, the second group received Rev 1 and the third group was inoculated with sterile physiological saline solution. The dose of both strains was of 1 × 109 CFU/ml. Over the course of the five months of this study, three males from each group were euthanized every month. Their reproductive tracts, spleens, and lymph nodes were collected to analyze serology, bacteriology PCR, histology, and immunohistochemistry. Results show that vaccination with B. melitensis strains Rev 1 ΔeryCD and Rev 1 does not harm the reproductive system of male goats. Strain B. melitensis Rev 1 ΔeryCD displayed a lower capacity to colonize the reproductive tract than strain Rev 1, which was attributed to its limited catabolic action toward erythritol.

接种布鲁氏菌 Rev 1 ΔeryCD 株和 Rev 1 株对年轻公山羊生殖系统的影响
本研究评估了接种 Rev 1 株和 Rev 1 株疫苗对雄性山羊生殖系统的影响。研究了三组山羊,每组包括 15 只 6 个月大无布鲁氏菌病的雄性山羊。第一组接种 Rev 1 株,第二组接种 Rev 1 株,第三组接种无菌生理盐水(对照组)。两种接种菌株的滴度均为 1×10UFC/ml。在五个月的研究期间,每月每组各牺牲三只雄性。采集生殖道、脾脏和淋巴结进行血清学、细菌学、PCR、组织学和免疫组化检查。结果表明,接种 Rev 1 株和 Rev 1 株疫苗不会对山羊的生殖系统造成损害。与 Rev 1 株相比,Rev 1 株由于对赤藓糖醇的分解作用有限,在生殖道的定植能力较弱。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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