Analysis of Escherichia coli isolated from minced meat: Implications for public health in Tierra del Fuego, Argentina.

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia Pub Date : 2025-06-19 DOI:10.1016/j.ram.2025.05.001

Elisa Crespi, Bárbara Ghiglione, Ximena Blanco Crivelli, Cecilia Cundon, Roque Figueroa Espinosa, Pedro Penzotti, Ezequiel Nuske, Alicia Broglio, Luis Ambros, María Valeria Rumi, Gabriela Albarellos, José Di Conza, Adriana Bentancor

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Abstract

Escherichia coli contamination in minced meat poses a significant public health concern and may serve as a reservoir for antimicrobial-resistant strains. This study investigated the presence of E. coli resistant to ciprofloxacin and/or gentamicin in minced meat samples from Tierra del Fuego, Argentina. Resistant E. coli isolates were identified using MALDI-TOF. A total of 109 samples were analyzed, yielding 97 E. coli isolates (23 from AMC-CIP and 74 from AMC-GEN). Twenty-three isolates carried the ant(2″) gene, ten the ant(3″) gene, and one the blaCTX-M gene. Resistance was observed to gentamicin (6/25), streptomycin (1/25), ceftazidime (1/25), and cefotaxime (4/25). Eleven isolates carried the aggR gene, and another three were identified as biofilm producers. Although phenotypic resistance was low, we detected genetic potential for resistance which, combined with biofilm formation, represents a risk to food safety.

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从肉糜中分离的大肠杆菌分析：对阿根廷火地岛公共卫生的影响。

肉糜中的大肠杆菌污染引起了重大的公共卫生问题，并可能成为耐抗生素菌株的储存库。本研究调查了阿根廷火地岛肉糜样品中对环丙沙星和/或庆大霉素耐药的大肠杆菌的存在。采用MALDI-TOF法鉴定耐药大肠杆菌分离株。共分析109份样品，分离出97株大肠杆菌（其中23株来自AMC-CIP， 74株来自AMC-GEN）。23株分离物携带蚂蚁（2″）基因，10株分离物携带蚂蚁（3″）基因，1株分离物携带blaCTX-M基因。对庆大霉素（6/25）、链霉素（1/25）、头孢他啶（1/25）、头孢噻肟（4/25）耐药。11株分离株携带aggR基因，另外3株被鉴定为生物膜生产者。虽然表型抗性较低，但我们检测到抗性的遗传潜力，结合生物膜的形成，对食品安全构成风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Argentina de microbiologia MICROBIOLOGY-

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： La Revista Argentina de Microbiología es una publicación trimestral editada por la Asociación Argentina de Microbiología y destinada a la difusión de trabajos científicos en las distintas áreas de la Microbiología. La Asociación Argentina de Microbiología se reserva los derechos de propiedad y reproducción del material aceptado y publicado.