Revista Argentina de microbiologia最新文献

{"title":"Primer caso autóctono de fiebre manchada asociado a la Reserva Ecológica Costanera Sur, Ciudad Autónoma de Buenos Aires","authors":"","doi":"10.1016/j.ram.2024.06.001","DOIUrl":"10.1016/j.ram.2024.06.001","url":null,"abstract":"<div><p>The first autochthonous case of rickettsiosis is reported here. The case occurred in the Costanera Sur Ecological Reserve, a protected area of the City of Buenos Aires, in August 2022, where 4 species of ticks were found, namely <em>Amblyomma aureolatum</em>, <em>Ixodes auritulus sensu lato</em>, <em>Rhipicephalus sanguineus sensu stricto</em> and <em>Amblyomma triste.</em></p><p>The epidemiological, ecological, clinical and laboratory aspects that allowed timely diagnosis and appropriate treatment are also described.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000828/pdfft?md5=00f0f581658022972e07d5d945bf6286&pid=1-s2.0-S0325754124000828-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caracterización molecular de aislamientos de Listeria monocytogenes de origen humano y alimentario en Argentina, 2018-2023","authors":"","doi":"10.1016/j.ram.2024.03.002","DOIUrl":"10.1016/j.ram.2024.03.002","url":null,"abstract":"<div><p>Human listeriosis is an infectious disease caused by <em>Listeria monocytogenes</em>. The invasive form of this disease leads to a high rate of hospitalizations and fatality. The main mode of transmission is through contaminated ready-to-eat foods such as dairy, vegetables and meat products. The knowledge of the diversity and population dynamics of isolates collected from human and food sources is essential for the detection of clusters and the identification of common sites of infection. The aim of this study was the molecular characterization of <em>L. monocytogenes</em> isolates in Argentina. We sequenced a total of 63 isolates, 35 from human and 28 from food sources, collected between 2018 and 2023. Our genomic study divided the isolates into two lineages, four serogroups, 17 sequence types and 15 clonal complexes (CCs). The hypervirulent clone CC1 (lineage I; serogroup IVb) predominated in human and food samples. The phylogenomic analysis showed a high and possible epidemiological relationship between isolates from human and/or food sources, suggesting the presence of transmission chains in our country. These findings highlight the need to strengthen genomic surveillance of <em>L. monocytogenes</em> in Argentina. The identification of geographic distribution and characteristics of predominant and emerging clones from human and food sources might help to focus action plans and public health policies better directed at the control and prevention of listeriosis.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000427/pdfft?md5=30bf52e2d9d8339ac85d9f6231190014&pid=1-s2.0-S0325754124000427-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Los fructanos de agave promueven la formación de biopelícula in vitro en el consorcio probiótico Lactobacillus delbrueckii ssp. lactis, L. delbrueckii ssp. bulgaricus y Streptococcus thermophilus","authors":"","doi":"10.1016/j.ram.2024.02.002","DOIUrl":"10.1016/j.ram.2024.02.002","url":null,"abstract":"<div><p>In recent years the relationship between the intestinal microbiota, the host and chronic non-communicable diseases has brought interest into the study of its formation and maintenance in the host. Lactic acid bacteria (BAL) are Gram-positive bacteria with probiotic activity, which have been associated with many health benefits, such as decreased body fat mass and lower risk of type II diabetes mellitus. One of the main colonization mechanisms and bacteria survival strategies is the production of biofilms and the use of prebiotics as substrates to achieve a balance within intestinal microbiota. However, there is not enough evidence to demonstrate the biofilm formation in the presence of agave fructans (AF). This study aimed to evaluate <em>in vitro</em> the biofilm formation in a consortium of lactic acid bacteria: <em>Lactobacillus delbrueckii</em> ssp. <em>lactis</em>, <em>Lactobacillus delbrueckii</em> ssp<em>. bulgaricus</em> y <em>Streptococcus thermophilus</em> in the presence of AF at different concentrations: 0%, 0,1%, 4%, 8% y 16%. The addition of 0,1% of AF correlates with the best capacity for biofilm formation. The findings imply the possibility of modulating the biofilm formation of lactic acid bacteria with AF. These results can contribute positively to the host, by generating intestinal homeostasis, colonization resistance, stability to food digestion and chemical modifications of drugs and carry out beneficial functions to the health.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S032575412400021X/pdfft?md5=7219cc8e88340733c0eaa2a67d5aa82b&pid=1-s2.0-S032575412400021X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140787164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are Mycoplasma pneumoniae coinfections frequent in COVID-19 patients? A systematic review","authors":"","doi":"10.1016/j.ram.2024.05.002","DOIUrl":"10.1016/j.ram.2024.05.002","url":null,"abstract":"<div><p>Understanding the proportion of SARS-CoV-2 patients with <em>Mycoplasma</em> <em>pneumoniae</em> coinfection is crucial for treating patients suffering from coronavirus disease (COVID-19), help to ensure responsible use of antibiotics and minimize the negative consequences of overuse. In addition, this knowledge could have an impact on empirical antibiotic management guidelines for patients with COVID-19. This systematic review aimed to identify the prevalence of <em>M. pneumoniae</em> in patients with coronavirus disease 2019 (COVID-19).</p><p>A bibliographic search of studies published in Spanish or English was conducted using the PubMed search engine. Fourteen articles from different continents (America, Asia and Europe) were included, involving a total of 5855 patients in these studies. The mean age of COVID-19 patients with <em>M. pneumoniae</em> was 48 years old (range 1–107), most of whom were male. The detection of laboratory-confirmed <em>M. pneumoniae</em> infection varied between 0 and 33.3%. Most of patients referred fever, cough, and dyspnea, and received empirical antibiotic treatment. Bacterial coinfection was not associated with increased ICU admission and mortality. The prevalence of coinfection showed extremely dissimilar figures according to the population studied and diagnostic criteria. However, it is important to develop Latin American studies, given the heterogeneity observed in the studies conducted in different countries. Standardized definitions should be developed in order to be able to assess the impact of coinfections in patients with a diagnosis of COVID-19.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000579/pdfft?md5=3b034561413e71059901f11d0e326c22&pid=1-s2.0-S0325754124000579-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arthrobacter sp. UMCV2, and its compound N,N-dimethylhexadecilamine promote nodulation in Medicago truncatula by Sinorhizobium medicae","authors":"","doi":"10.1016/j.ram.2024.03.004","DOIUrl":"10.1016/j.ram.2024.03.004","url":null,"abstract":"<div><p>The actinobacterium <em>Arthrobacter</em> sp. UMCV2 promotes plant growth through the emission of <em>N</em>,<em>N</em>-dimethylhexadecilamine (DMHDA). The <em>Medicago</em>–<em>Sinorhizobium</em> nodulation has been employed to study symbiotic nitrogen fixation by rhizobia in nodulating Fabaceae. Herein, we isolated three <em>Sinorhizobium medicae</em> strains that were used to induce nodules in <em>Medicago truncatula</em>. The co-inoculation of <em>M. truncatula</em> with <em>Arthrobacter</em> sp. strain UMCV2 produced a higher number of effective nodules than inoculation with only <em>Sinorhizobium</em> strains. Similarly, the exposure of inoculated <em>M. truncatula</em> to DMHDA produced a greater number of effective nodules compared to non-exposed plants. Thus, we conclude that <em>Arthrobacter</em> sp. UMCV2 promotes nodulation, and propose that this effect is produced, at least partly, <em>via</em> DMHDA emission.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000440/pdfft?md5=84f0ab65fec625828e1d01d64c6ddff5&pid=1-s2.0-S0325754124000440-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141176249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyanobacteria in soils under dryland agricultural production systems","authors":"","doi":"10.1016/j.ram.2024.03.003","DOIUrl":"10.1016/j.ram.2024.03.003","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000439/pdfft?md5=4dfffc4981b16b4081d100a7246a305c&pid=1-s2.0-S0325754124000439-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors and mortality of candidemia in a children's public hospital in Sao Paulo, Brazil","authors":"","doi":"10.1016/j.ram.2023.09.002","DOIUrl":"10.1016/j.ram.2023.09.002","url":null,"abstract":"<div><p><em>Candida</em> bloodstream infections in children are of special concern in neonatal and pediatric intensive care and patients with comorbidities. This study aimed to estimate the incidence and risk factors associated with mortality in candidemia cases occurring in a public children's hospital in Ribeirao Preto, Brazil. It is a retrospective transversal study. Every patient under the age of 18 admitted to the study facility from January 1, 2013, to December 31, 2019, was considered potentially eligible to be included if they had candidemia. We collected clinical data from medical records. We included 113 blood cultures yielding positive results for <em>Candida</em>. The incidence rate was 2.12 per 1000 admissions. The most common <em>Candida</em> species was <em>Candida parapsilosis</em>. Septic shock during the candidemia episode was the only clinical outcome associated with a relative risk-adjusted (RRa) of 2.77 with an interval &gt;1 (1.12–6.85). Our findings show that the incidence rate and mortality rates of candidemia are in line with those in other children's services in Brazil. We found a global mortality rate of 28.31% (32/113) from candidemia episodes. We highlight the predominance of non-albicans <em>Candida</em> species including <em>C. parapsilosis</em>. Septic shock was the most important factor showing a significant risk of mortality.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000397/pdfft?md5=6754201fe6750d6241830260f8b16981&pid=1-s2.0-S0325754124000397-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140757970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a mixed additive based on Saccharomyces cerevisiae and Lactobacillus rhamnosus on broilers exposed to aflatoxin B1 by contaminated feed","authors":"","doi":"10.1016/j.ram.2023.11.006","DOIUrl":"10.1016/j.ram.2023.11.006","url":null,"abstract":"<div><p>The objective of the present study was to explore the influence of dietary supplementation with a mixed additive (MA) containing a probiotic and anti-mycotoxin (<em>Saccharomyces cerevisiae</em> RC016 and <em>Lactobacillus rhamnosus</em> RC007) and its interaction on the performance and health (biochemistry and liver/intestine histopathology) of broilers fed diets contaminated with aflatoxin B₁ (AFB₁) at 506<!--> <!-->000<!--> <!-->±<!--> <!-->22.1<!--> <!-->ng/kg. The MA contained <em>S. cerevisiae</em> RC016 (1<!--> <!-->×<!--> <!-->10⁷ <!-->cells/g) and <em>L. rhamnosus</em> RC007 (1<!--> <!-->×<!--> <!-->10⁸ <!-->cells/g) in relation 1:1. A total of sixty-one-day-old Cobb broilers were randomly allocated into four treatment groups with three replicates of 5 birds each for a five-week-old feeding experiment. The experimental diet for each treatment (T) was formulated as follows: T1, a commercial diet (CD); T2, CD<!--> <!-->+<!--> <!-->AFB₁; T3, CD<!--> <!-->+<!--> <!-->0.1% MA; T4, CD<!--> <!-->+<!--> <!-->AFB₁ <!-->+<!--> <!-->0.1% MA. The MA improved (<em>p</em> <!-->&lt;<!--> <!-->0.01) production parameters (weight gain, conversion rate, and carcass yield) and reduced (<em>p</em> <!-->&lt;<!--> <!-->0.01) the toxic effect of AFB₁ on the relative weight of the livers. In addition, the macro and microscopic alterations of livers and the possible intestinal injury related to histological damage in the presence of mycotoxin were reduced. The use of probiotic MA based on <em>S. cerevisiae</em> RC016 and <em>L. rhamnosus</em> RC007 in animal feed provides greater protection against mycotoxin contamination and is safe for use as a supplement in animal feed, providing beneficial effects that improve animal health and productivity. This is of great importance at the economic level for the avian production system.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000804/pdfft?md5=68ff0cace867ee2cdecf71f3b49554e1&pid=1-s2.0-S0325754124000804-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of monkeypox virus in central Argentina: Epidemiological features and first complete genome sequences in the country","authors":"","doi":"10.1016/j.ram.2024.05.003","DOIUrl":"10.1016/j.ram.2024.05.003","url":null,"abstract":"<div><p>Monkeypox (Mpox) is a zoonotic disease caused by the monkeypox virus (MPXV). MPXV can be transmitted by close contact with lesions, body fluids, respiratory droplets, and contaminated materials. A new pattern of spread among sexual networks has been recently described. The present work aimed to report the epidemiological and genomic characterization of the 2022 MPXV outbreak in central Argentina. A total of 113 scabs and/or lesion swab specimens were studied. MPXV infection was confirmed in 46.0% of the studied patients, all of whom were men. Varicella-zoster virus infection was the most frequent differential diagnosis. Eight complete viral genomes were obtained by next-generation sequencing. The Argentinian sequences were grouped intermingled with other sequences from the 2022 MPXV outbreak, related to samples from the USA, Europe, and Peru. Taken together, our study provided an initial assessment of the genetic and epidemiological characteristics of the 2022 MPXV outbreak in Córdoba, Argentina.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000774/pdfft?md5=19901670a2a3199969206463fb17a33b&pid=1-s2.0-S0325754124000774-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of lineage III of Shigella sonnei ST152 belonging to a high-risk clone harboring the blaCTX-M-15 gene in Peru","authors":"","doi":"10.1016/j.ram.2024.02.007","DOIUrl":"10.1016/j.ram.2024.02.007","url":null,"abstract":"<div><p>Multidrug-resistant <em>Shigella sonnei</em> ST152, global lineage III, is a high-risk clone, whose dissemination has limited therapeutic options for shigellosis. This study aimed to characterize two isolates of <em>S. sonnei</em>, which were recovered in Lima, Peru, during November 2019, exhibiting resistance to extended-spectrum cephalosporins and quinolones, and concurrently harboring <em>bla</em>_CTX-M-15 and <em>qnrS1</em> genes, in addition to mutations in <em>gyrA</em>-S83L. These isolates were resistant to ceftriaxone, ciprofloxacin and trimethoprim/sulfamethoxazole. The molecular analysis showed that both isolates belonged to lineage III, sublineages IIIa and IIIb. The <em>bla</em>_CTX-M-15 gene was located in the same genetic platform as <em>qnrS1</em>, flanked upstream by ISKpn19, on a conjugative plasmid belonging to the IncI-γ group. To the best of our knowledge, this would be the first report on <em>S. sonnei</em> isolates carrying the <em>bla</em>_CTX-M-15 gene in Peru. The global dissemination of <em>S. sonnei</em> ST152, co-resistant to β-lactams and quinolones, could lead to a worrisome scenario in the event of potential acquisition of genetic resistance mechanisms to azithromycin.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000403/pdfft?md5=6c80b341bbe0d9cd0ab43a7c2bf50852&pid=1-s2.0-S0325754124000403-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}