Revista Argentina de microbiologia最新文献_第2页

Optimized DNA extraction protocol for Staphylococcus aureus strains utilizing liquid nitrogen 利用液氮优化金黄色葡萄球菌DNA提取工艺。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2024.12.006

Mariana B. Galeano , Stefania A. Robaldi , Tania B. Gordillo , Martiniano M. Ricardi , Pablo M. Cassanelli , Rosana O. Pereda , Maria Mercedes Palomino , Paula Maria Tribelli

{"title":"Optimized DNA extraction protocol for Staphylococcus aureus strains utilizing liquid nitrogen","authors":"Mariana B. Galeano , Stefania A. Robaldi , Tania B. Gordillo , Martiniano M. Ricardi , Pablo M. Cassanelli , Rosana O. Pereda , Maria Mercedes Palomino , Paula Maria Tribelli","doi":"10.1016/j.ram.2024.12.006","DOIUrl":"10.1016/j.ram.2024.12.006","url":null,"abstract":"<div><div>DNA extraction is crucial for conducting procedures, such as whole-genome sequencing, which demand methods that are reproducible and cost-effective. Lysing <em>Staphylococcus aureus</em> cells is particularly challenging due to their peptidoglycan layer that is resistant to common treatments. Traditional methods involve costly enzymatic lysis using lysostaphin. Here, we developed a novel approach for lysis utilizing liquid nitrogen and mechanical disruption in a mortar. DNA from <em>S. aureus</em> USA300 and related clinical isolates were purified using phenol–chloroform extraction followed by precipitation. The integrity and purity of DNA were confirmed, obtaining suitable concentration and purity for various molecular biology techniques. The quality of the employed DNA was validated by amplifying fragments of different genes using PCR. This method circumvents lysostaphin, yielding DNA that is suitable for use in other techniques.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 217-220"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an enzyme-linked immunosorbent assay for detection of antibodies against infectious bursal disease virus based on a version of the recombinant viral protein 2 基于重组病毒蛋白2的酶联免疫吸附法检测传染性法氏囊病病毒抗体的建立

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2024.09.003

Leticia Keller, Carina Romanutti, Flavia Adriana Zanetti

{"title":"Development of an enzyme-linked immunosorbent assay for detection of antibodies against infectious bursal disease virus based on a version of the recombinant viral protein 2","authors":"Leticia Keller, Carina Romanutti, Flavia Adriana Zanetti","doi":"10.1016/j.ram.2024.09.003","DOIUrl":"10.1016/j.ram.2024.09.003","url":null,"abstract":"<div><div>Infectious bursal disease virus (IBDV) is the etiological agent of a highly contagious and immunosuppressive disease in chickens. In poultry farms, the level of anti-IBDV antibodies of numerous serum samples must be monitored using fast and simple methodologies. Therefore, the aim of this study was to develop an enzyme-linked immunosorbent assay (ELISA), in an indirect format, using a version of mature viral protein 2 (VP2) of IBDV as coating agent. This recombinant fusion protein (His-VP2) was expressed at high levels in <em>Escherichia coli</em>. Bacterial inclusion bodies containing His-VP2 were successfully recovered using a simple, inexpensive and efficient method, a further purification of recombinant protein by affinity chromatography using immobilized metal chelates being unnecessary. After the VP2-ELISA was optimized, its performance was evaluated using preanalyzed sera from uninfected specific pathogen-free chickens and broilers vaccinated against IBDV in poultry farms, using a commercial ELISA kit. Based on these results, the developed assay proved to be sensitive, specific and in high agreement with the kit available on the market. In addition, the in-house ELISA demonstrated to be reproducible by intra-assay and inter-assay variability studies. In conclusion, VP2-ELISA could be an efficient and low-cost alternative diagnostic method to detect antibodies to IBDV in the poultry industry.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 208-216"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Composition in phyla from breast milk: Effect of the mode of delivery 母乳中细菌门的组成：分娩方式的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2025.04.001

Shiva Aslani , Seyedeh Neda Mousavi , Atefeh Kazemi Robati , Siamak Heidarzadeh , Somaye Abdollahi Sabet

{"title":"Composition in phyla from breast milk: Effect of the mode of delivery","authors":"Shiva Aslani , Seyedeh Neda Mousavi , Atefeh Kazemi Robati , Siamak Heidarzadeh , Somaye Abdollahi Sabet","doi":"10.1016/j.ram.2025.04.001","DOIUrl":"10.1016/j.ram.2025.04.001","url":null,"abstract":"<div><div>Breast milk is considered as a living ecosystem. Maternal, environmental and neonatal factors affect milk bacterial composition. The aim of present study was to assess the phyla from breast milk of mothers with vaginal delivery compared to the cesarean section. In this single-center case–control study, sixty women were participated. Half of them had vaginal delivery and others experienced cesarean section. The breast milk samples were collected three months after delivery for the DNA extraction to measure Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria using quantitative real time chain polymerase reaction. Daily intake of calories, protein, fat, carbohydrate and fiber did not differ significantly between the two groups. The proportion of Bacteroidetes, Firmicutes and Proteobacteria were significantly higher in milk of mothers with a cesarean section than the vaginal delivery (<em>p</em>  =0.02 and <em>p</em>  =0.02). The Actinobacteria population was significantly higher in milk of vaginally-delivered mothers who had male infant than females (<em>p</em>  <!-->0.015). Breast milk of mothers with cesarean section showed alterations in the main bacterial phyla population compared to the vaginal delivery. Moreover, our results suggest that the sex of infant is an effective factor on some phyla quantity.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 231-238"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriemia por Salmonella enterica no Typhi atípica, lactosa positiva, lisina decarboxilasa negativa [非典型非Typhi肠炎沙门氏菌菌血症，乳糖阳性，赖氨酸脱羧酶阴性]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2024.09.007

Susana D. García , María I. Caffer , Marisa N. Almuzara , Ángela M.R. Famiglietti , Cristina Myburg , María R. Viñas , Jorge Risso , Carlos A. Vay

引用次数: 0

In vitro activity of ceftobiprole against Staphylococcus aureus: Current experience 头孢替比罗对金黄色葡萄球菌的体外活性：目前的经验。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2024.12.011

Manuel Paz-Infanzon , Adrian Camacho-Ortiz , Eduardo Perez-Alba , Laura Nuzzolo-Shihadeh , Rayo Morfin-Otero , Paola Bocanegra-Ibarias

引用次数: 0

Analysis of Escherichia coli isolated from minced meat: Implications for public health in Tierra del Fuego, Argentina. 从肉糜中分离的大肠杆菌分析：对阿根廷火地岛公共卫生的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-06-19 DOI: 10.1016/j.ram.2025.05.001

Elisa Crespi, Bárbara Ghiglione, Ximena Blanco Crivelli, Cecilia Cundon, Roque Figueroa Espinosa, Pedro Penzotti, Ezequiel Nuske, Alicia Broglio, Luis Ambros, María Valeria Rumi, Gabriela Albarellos, José Di Conza, Adriana Bentancor

{"title":"Analysis of Escherichia coli isolated from minced meat: Implications for public health in Tierra del Fuego, Argentina.","authors":"Elisa Crespi, Bárbara Ghiglione, Ximena Blanco Crivelli, Cecilia Cundon, Roque Figueroa Espinosa, Pedro Penzotti, Ezequiel Nuske, Alicia Broglio, Luis Ambros, María Valeria Rumi, Gabriela Albarellos, José Di Conza, Adriana Bentancor","doi":"10.1016/j.ram.2025.05.001","DOIUrl":"10.1016/j.ram.2025.05.001","url":null,"abstract":"<p><p>Escherichia coli contamination in minced meat poses a significant public health concern and may serve as a reservoir for antimicrobial-resistant strains. This study investigated the presence of E. coli resistant to ciprofloxacin and/or gentamicin in minced meat samples from Tierra del Fuego, Argentina. Resistant E. coli isolates were identified using MALDI-TOF. A total of 109 samples were analyzed, yielding 97 E. coli isolates (23 from AMC-CIP and 74 from AMC-GEN). Twenty-three isolates carried the ant(2″) gene, ten the ant(3″) gene, and one the blaCTX-M gene. Resistance was observed to gentamicin (6/25), streptomycin (1/25), ceftazidime (1/25), and cefotaxime (4/25). Eleven isolates carried the aggR gene, and another three were identified as biofilm producers. Although phenotypic resistance was low, we detected genetic potential for resistance which, combined with biofilm formation, represents a risk to food safety.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk factors associated with neonatal sepsis due to Serratia marcescens in a public hospital in Mexico: A case-control study. 墨西哥公立医院粘质沙雷菌所致新生儿败血症的相关危险因素：一项病例对照研究

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-06-11 DOI: 10.1016/j.ram.2025.04.006

Juan Carlos Lona-Reyes, Lucia Miramontes-Buiza, Luis Fernando Frías-Flores, Jose Angel Regla-Nava, Jesús González-Carmona, Juan Antonio Gallegos-Marín, Mariana Chávez-Rodríguez

引用次数: 0

Effect of probiotic bacteria, phycocyanin and tarragon essential oil on the physiochemical, microbiological, and sensory properties of a mouth freshener tablet. 益生菌、藻蓝蛋白和龙蒿精油对口腔清新片理化、微生物学和感官特性的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-05-28 DOI: 10.1016/j.ram.2025.04.004

Fatemeh Habibi, Bahareh Nowruzi, Maryam Ghane

{"title":"Effect of probiotic bacteria, phycocyanin and tarragon essential oil on the physiochemical, microbiological, and sensory properties of a mouth freshener tablet.","authors":"Fatemeh Habibi, Bahareh Nowruzi, Maryam Ghane","doi":"10.1016/j.ram.2025.04.004","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.004","url":null,"abstract":"<p><p>Microbial pigments are extensively used in industry as food coloring, dyes, and medicinal substances. This study aimed to develop a probiotic mouth freshener tablet containing PC, tarragon essential oil, and microencapsulated Lactobacillus plantarum and investigate its antimicrobial properties against Streptococcus mutans. First, cyanobacterium Spirulina sp. was cultured and PC extracted. Then, microbial suspension and microencapsulation of probiotic bacteria, tarragon essential oil extraction, as well as mouth freshener tablets were prepared. Four treatments (T) under Co: control sample, T<sub>1</sub>, T<sub>2</sub>, T<sub>3</sub>, and T<sub>4</sub> contained 1% PC, 1% microcoating probiotic, 0.8% tarragon essential oil, and 1% PC+1% microcoating probiotic+0.8% tarragon essential oil, were prepared respectively. The results of the inhibition zone diameter of samples also indicated that the highest and lowest sensitivity were observed in T<sub>4</sub> and T<sub>1</sub>, respectively. Based on the results, the addition of PC and tarragon essential oil significantly increased the antioxidant activity of the samples (p<0.05). The results indicated that the release rate of probiotic bacteria under gastric conditions in T<sub>4</sub> was lower compared to that in treatment T<sub>2</sub> (p<0.05). Sensory evaluation results showed that the highest values were observed in T<sub>3</sub> and T<sub>4</sub> (p<0.05). CONCLUSION: Overall, it can be concluded that the prepared mouth freshener tablets (T<sub>4</sub>) can be effective agents for oral disinfection and the promotion of public health.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mosquito-borne pathogens … Now Rickettsias? 蚊子传播的病原体…现在是立克次体？

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-05-26 DOI: 10.1016/j.ram.2025.03.002

Silvina Goenaga, Estefania Raquel Boaglio, Lucia Victoria Martinez, Juliana Patricia Sanchez

引用次数: 0

Toxicity assessment of Bacillus thuringiensis strains for the control of the lesser mealworm beetle Alphitobius diaperinus (Coleoptera: Tenebrionidae). 苏云金芽孢杆菌菌株对小粉虫甲（鞘翅目：拟粉虫科）的毒力评价。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-05-22 DOI: 10.1016/j.ram.2025.03.003

Melisa P Pérez, Graciela B Benintende, Diego H Sauka

引用次数: 0