Revista Argentina de microbiologia最新文献_第2页

Antifungal and antioxidant potential of Ocimum species against Ascochyta rabiei (Pass) Lab 茜草属植物抗蚜活性及抗氧化活性研究。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2025.02.003

Tooba Shafiq , Khajista Jabeen , Sumera Iqbal , Aisha Umar , Mohamed S. Elshikh , Reem M. Aljowaie , Laurent Dufossé , Rashid Iqbal , Soumya Ghosh

{"title":"Antifungal and antioxidant potential of Ocimum species against Ascochyta rabiei (Pass) Lab","authors":"Tooba Shafiq , Khajista Jabeen , Sumera Iqbal , Aisha Umar , Mohamed S. Elshikh , Reem M. Aljowaie , Laurent Dufossé , Rashid Iqbal , Soumya Ghosh","doi":"10.1016/j.ram.2025.02.003","DOIUrl":"10.1016/j.ram.2025.02.003","url":null,"abstract":"<div><div><em>Cicer arietinum</em> L. is a vital source of nutrients that suffers substantial annual losses due to Ascochyta blight, caused by the plant pathogen <em>Ascochyta rabiei</em>. This study aimed to investigate the antifungal potential of <em>Ocimum tenuiflorum</em> L. and <em>O. basilicum</em> L. shoots (leaves and stems) against <em>A. rabiei</em> (Pass) Lab. <em>In vitro</em> bioassays were conducted using methanolic extracts from leaves and stems at six different concentrations: 1%, 1.5%, 2%, 2.5%, 3%, and 3.5%. A total of eight compounds were identified through gas chromatography–mass spectrometry (GC–MS) analysis. The highest inhibition of <em>A. rabiei</em> growth was achieved with a 3.5% methanolic leaf extract of <em>O. basilicum</em>. Methanolic extracts from <em>O. tenuiflorum</em> shoots also reduced fungal growth by 6.18–73%. Additionally, the n-hexane fraction derived from <em>O. basilicum</em> inhibited fungal growth by 71–76% and was subsequently analyzed using GC–MS. This analysis identified eight compounds: (1) cyclopentane, methyl-, (2) cyclohexane, (3) 2,2-dimethylbutane, (4) 2,3-dimethylbutane, pentane, (5) 2,3-dimethyl-, (6) 2-bromoacetonitrile, (7) alpha-cadinol, and (8) phenylpropanolamine. The antioxidant activity of <em>O. tenuiflorum</em> and <em>O. basilicum</em> shoots was also assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The highest antioxidant activity, 98.58%, was recorded at a 3.5% methanolic stem extract concentration of <em>O. tenuiflorum</em>. The antioxidant activity potential was highest for <em>O. tenuiflorum</em> at 0.729mg/mL, followed by <em>O. basilicum</em> at 0.411mg/mL.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 125-135"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factores de riesgo asociados a la presentación de brucelosis en establecimientos mixtos de caprinos-bovinos de la provincia de Formosa, Argentina [阿根廷福莫萨省混合饲养场（山羊和牛）出现布鲁氏菌病的相关风险因素]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.10.003

Iris Carolina Elías , María Nair Viola , Ana Maria Russo , Marcelo Signorini Porchietto

{"title":"Factores de riesgo asociados a la presentación de brucelosis en establecimientos mixtos de caprinos-bovinos de la provincia de Formosa, Argentina","authors":"Iris Carolina Elías , María Nair Viola , Ana Maria Russo , Marcelo Signorini Porchietto","doi":"10.1016/j.ram.2024.10.003","DOIUrl":"10.1016/j.ram.2024.10.003","url":null,"abstract":"<div><div>Brucellosis is a zoonotic disease with a worldwide distribution that causes significant productive losses and negative impacts on public health. The aim of this work was to identify the risk factors associated with the presence of brucellosis in mixed farms (cattle/goats) in the central-western region of the province of Formosa. Sixty seven mixed farms with 7,855 animals (2,943 cattle and 4,912 goats) were randomly sampled during 2022/2023. The samples were serologically analyzed (BPA + FPA) to determine which animals were positive. Likewise, in each farm, a confidential survey was carried out to evaluate potential associated variables. The statistical analysis was performed using Generalized Linear Mixed Models (GLMM) with binomial distribution, logarithmic link function, and farm as a random variable. The estimated prevalence of brucellosis in mixed farms was 9%, while the joint prevalence per animal (cattle and goats) was 2.75%. The prevalence of brucellosis in cattle and goats was explained by the presence of animals with reproductive signs (<em>odds ratio</em>, OR=40), the occurrence of abortions in the last term of gestation and the birth of weak offspring (OR=5.3), incorrect treatment of abortions (OR=8), the introduction of animals from other farms (OR=5.9), and not having a negative brucellosis certificate (OR=9.6). The management practices conducted in each farm such as preventive measures, sanitation practices for the pens, and prevention measures for the workers, are important for the control of brucellosis.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 152-160"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacto de los marcadores microbiológicos en la evolución de la infección por Clostridioides difficile [微生物标志物在艰难梭菌感染进展中的作用]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.12.003

María Dolores Rojo-Martín , Juan Diego Ruiz-Mesa , Concepción Mediavilla-Gradolph , Mercedes Marín-Arriaza , Alexandra Halbardier-Carretero , José Serrano-Sánchez , Ignacio Márquez-Gómez , Inmaculada de Toro-Peinado , Luis Francisco Caballero-Martínez , Aurora García-Barrionuevo , Begoña Palop-Borrás

{"title":"Impacto de los marcadores microbiológicos en la evolución de la infección por Clostridioides difficile","authors":"María Dolores Rojo-Martín , Juan Diego Ruiz-Mesa , Concepción Mediavilla-Gradolph , Mercedes Marín-Arriaza , Alexandra Halbardier-Carretero , José Serrano-Sánchez , Ignacio Márquez-Gómez , Inmaculada de Toro-Peinado , Luis Francisco Caballero-Martínez , Aurora García-Barrionuevo , Begoña Palop-Borrás","doi":"10.1016/j.ram.2024.12.003","DOIUrl":"10.1016/j.ram.2024.12.003","url":null,"abstract":"<div><div>The main current diagnostic challenge in <em>Clostridioides difficile</em> infection (CDI) is to detect patients with the poorest prognoses. We have studied the association between the presence of toxin in the feces, the amplification of the toxin B gene at low cycles in PCR (Ct), and the detection of the binary toxin or ribotype with severity, recurrence, and poor prognosis. Toxigenic <em>C. difficile</em> has been investigated in adult diarrheal feces, regardless of the clinical request. Glutamate dehydrogenase (GDH), toxin A (TcdA) and toxin B (TcdB) have been assessed by immunoassay and the gene encoding toxin B (tcdB) and the binary toxin (cdtA) by PCR. The toxigenic strains recovered by toxigenic culture were typed by PCR-Ribotyping and subtyped by MLVA (multilocus-variable number tandem DNA repeat analysis). Eighty-two (82) cases of CDI were detected from 677 samples (12.1%). Patients with Ct ≤ 27.5 often presented criteria for severity, recurrence, and poor prognosis (59.3% versus 40.7%, <em>P</em>=.15; 80% versus 20%, <em>P</em>=.10; and 60.3% versus 39.7%, <em>P</em>=.055, respectively). The mean Ct was lower when recurrence was observed (25.48; SD=2.41; <em>P</em>=.058). By logistic regression, the variable most likely to indicate a poor prognosis was Ct ≤ 27.5 (OR=2.663; 95% CI=.983-7.636; <em>P</em>=.059). In summary, the cycle threshold of the PCR for toxin B is a possible marker of severity, recurrence, and poor prognosis in CDI. The diversity of ribotypes observed is remarkable, with no clonal relationship between isolates of the same ribotype.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 142-151"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gluconobacter spp. y Paenibacillus polymyxa causan pudrición en zanahorias comerciales aparentemente sanas [葡萄球菌和多粘类芽孢杆菌会导致看起来健康的商品胡萝卜变质]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.12.009

Thelma J. Parra-Aguilar, María de Jesús Yáñez-Morales, Alejandra Almaraz-Sánchez, Alfonsina Judith Hernández, Sergio Aranda-Ocampo

{"title":"Gluconobacter spp. y Paenibacillus polymyxa causan pudrición en zanahorias comerciales aparentemente sanas","authors":"Thelma J. Parra-Aguilar, María de Jesús Yáñez-Morales, Alejandra Almaraz-Sánchez, Alfonsina Judith Hernández, Sergio Aranda-Ocampo","doi":"10.1016/j.ram.2024.12.009","DOIUrl":"10.1016/j.ram.2024.12.009","url":null,"abstract":"<div><div>Postharvest diseases of carrots are one of the most important concerns in carrot storage, as they affect the shelf life of carrots and cause significant financial losses. In this study, ten commercial carrot samples from supermarkets and local markets in Texcoco, State of Mexico, Mexico, were analyzed for bacterial spoilage after incubation at 28°C in a humidity chamber. Carrots from seven samples developed bacterial spoilage after five days of incubation, of which baby carrots (industrially processed) showed the most severe rot. Twenty-three bacterial strains were isolated from the tissues of spoiled carrots. To identify and characterize the spoilage pathogens, the isolated strains were inoculated into healthy carrots in laboratory tests. Of the 23 strains analyzed, eight caused carrot tissue spoilage. Biochemical and molecular characterization by 16S ribosomal RNA gene sequencing identified <em>Gluconobacter cerinus</em> (1032.2, 1059 and 1070.1), <em>G.</em> <em>kondonii</em> (1027.1), <em>G.</em> <em>wancherniae</em> (1033.1) and <em>Paenibacillus polymyxa</em> (1074.2, 1076 and 1077) as the causal agents of the carrot rot evaluated in this study. In addition, these bacteria showed virulence in other plant pathogenicity tests; <em>Gluconobacter</em> strains induced a hypersensitivity reaction in tobacco leaves and <em>Paenibacillus</em> strains showed pectolytic activity in potato tubers. This study is the first to report <em>G.</em> <em>cerinus</em>, <em>G.</em> <em>kondonii</em>, <em>G.</em> <em>wancherniae</em> and <em>P.</em> <em>polymyxa</em> as causal agents of commercial carrot rot.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 192-201"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dual impact of Trichoderma on soil microbial communities: beneficial or disruptive? 木霉对土壤微生物群落的双重影响：有益还是破坏性？

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2025.04.003

Adriana M. Torres

引用次数: 0

Use of stool molecular panel in hospitalized patients with diarrhea. Experience in a tertiary care center 粪便分子板在住院腹泻患者中的应用。有三级医疗中心工作经验。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.10.005

Nicolás Cimmino , Maria Josefina Etchevers , Astrid Smud , Patricia Yolleth Andrade Pino , Marisa del Lujan Sanchez , Maria Laura Gonzalez , Maria Victoria Acosta , Gissel Zuñiga , Karina Alejandra Perez , Juana Freggiaro , Valeria Rosa Alexander , Maria Florencia Veiga , Mariangeles Visus , Mariano Martin Marcolongo

{"title":"Use of stool molecular panel in hospitalized patients with diarrhea. Experience in a tertiary care center","authors":"Nicolás Cimmino , Maria Josefina Etchevers , Astrid Smud , Patricia Yolleth Andrade Pino , Marisa del Lujan Sanchez , Maria Laura Gonzalez , Maria Victoria Acosta , Gissel Zuñiga , Karina Alejandra Perez , Juana Freggiaro , Valeria Rosa Alexander , Maria Florencia Veiga , Mariangeles Visus , Mariano Martin Marcolongo","doi":"10.1016/j.ram.2024.10.005","DOIUrl":"10.1016/j.ram.2024.10.005","url":null,"abstract":"<div><div>Infectious diarrhea is a common health issue that affects a large number of individuals each year. It causes significant morbidity and mortality, greatly impacting healthcare system costs. Rapid detection of the causative organism and timely treatment alters the management and outcome of the condition. Molecular panels in stool allow to analyze a wide range of pathogens quickly and easily. For this study, a cross-sectional cohort analysis with a retrospective analysis of adult patients hospitalized with diarrhea and negative conventional stool bacteriological studies was conducted. Data obtained from the use of molecular panels in stool and the role of endoscopy in the diagnostic pathway were analyzed. A positivity rate of 52% (n=41) out of a total of 79 samples was reported. The test contributed to a change in therapeutic approach in 58% (n=46) of the patients. Among the patients with a negative molecular panel, 39.5% underwent further evaluation with colonoscopy involving biopsies, resulting in a diagnostic yield of 87%. Based on these results, we can conclude that molecular techniques contribute to the diagnosis and change in therapeutic approach in hospitalized patients with diarrhea.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 98-104"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endemic coronavirus in children and adults with acute respiratory infection before the COVID-19 pandemic COVID-19大流行前急性呼吸道感染儿童和成人中的地方性冠状病毒。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.11.003

Raquel Jara , Dafne Santos , Noelia Soledad Reyes , Eliana Hermida , Alejandro Seoane , Martin Ypas , Gisela Andres , Marcela Echavarría

{"title":"Endemic coronavirus in children and adults with acute respiratory infection before the COVID-19 pandemic","authors":"Raquel Jara , Dafne Santos , Noelia Soledad Reyes , Eliana Hermida , Alejandro Seoane , Martin Ypas , Gisela Andres , Marcela Echavarría","doi":"10.1016/j.ram.2024.11.003","DOIUrl":"10.1016/j.ram.2024.11.003","url":null,"abstract":"<div><div>Acute respiratory infection (ARI) is one of the principal causes of morbidity worldwide, with respiratory viruses being common etiological agents. Among them, endemic human coronaviruses (hCoVs) including CoV-229E, CoV-OC43, CoV-NL63, and CoV-HKU1 can cause mild ARI but are usually not evaluated in the clinical setting. The aim of this work was to determine the prevalence of all respiratory pathogens, with the focus placed on endemic hCoVs in the pre-pandemic period. Circulating species, clinical associations and coinfections with other respiratory pathogens were evaluated in 510 immunocompetent patients (children and adults) with ARI using the FilmArray® Respiratory Panel (BioFire/bioMérieux). A total of 399 children (252 outpatients and 147 hospitalized) and 111 adult outpatients were enrolled in the pre-pandemic period (2008–2010 and 2016). Endemic hCoVs were the third and fifth more frequently detected viruses among adults and outpatient children, respectively, with an overall frequency close to 10%. The most prevalent species were CoV-OC43 (42.8%) and CoV-HKU1 (40.5%), followed by CoV-NL63 (19.0%) and CoV-229E (4.8%). Tachypnea, wheezing and chest indrawing were more frequent in hospitalized children compared to outpatients. All adult patients presented with symptoms of a common cold. Endemic hCoVs were detected year-round, primarily between June and November. Our results highlight their clinical relevance, and the need to include endemic hCoVs in routine screening. In the post-pandemic period, further long-term surveillance is needed for understanding the epidemiology of endemic hCoVs and their evolution, as a tool to anticipate the possible emergence of new species.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 161-170"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identificación y sensibilidad antibiótica de aislamientos de Aeromonas spp. en un Hospital Universitario de la ciudad de Buenos Aires [布宜诺斯艾利斯某大学医院气单胞菌的鉴定及抗生素敏感性]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-04-01 DOI: 10.1016/j.ram.2024.11.001

Eduardo Montalvo , Florencia Veiga , Hernán Rodríguez , German Traglia , Carlos Vay , Marisa Almuzara

{"title":"Identificación y sensibilidad antibiótica de aislamientos de Aeromonas spp. en un Hospital Universitario de la ciudad de Buenos Aires","authors":"Eduardo Montalvo , Florencia Veiga , Hernán Rodríguez , German Traglia , Carlos Vay , Marisa Almuzara","doi":"10.1016/j.ram.2024.11.001","DOIUrl":"10.1016/j.ram.2024.11.001","url":null,"abstract":"<div><div><em>Aeromonas</em> spp. are opportunistic pathogens that cause both intra- and extraintestinal infections. The objective of this work was the phenotypic and genotypic characterization of a collection of <em>Aeromonas</em> strains, in addition to determining their sensitivity to different antimicrobials. Thirty seven isolates were analyzed. 54% were of intra-abdominal origin, 22% from skin and soft tissues, 19% from the bloodstream, among other less frequent sites. By amplification and sequencing of the <em>gyrB</em> gene, which was considered the reference method, the following were identified: 37,8% as species of the <em>Aeromonas hydrophila</em> complex, 32,4% as species of the <em>Aeromonas veronii</em> complex, and 29,7% as species of the complex <em>Aeromonas caviae</em>. Identification by traditional biochemical tests presented a better correlation with molecular identification than mass spectrometry (MALDI TOF MS). Regarding antibiotic sensitivity, cefotaxime, ceftazidime, cefepime, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, ciprofloxacin, amikacin, gentamicin and nitrofurantoin showed activity on more than 80.0% of the isolates tested. The sensitivity and specificity of the phenotypic methods to determine the presence of carbapenemases in relation to the detection of the cphAgene, the reference method, was 60,9% and 100%, respectively, for the colorimetric assay (Blue Carba), and of 91,3% and 50,0% respectively, for the modified Hodge test. The overall resistance to colistin was 32,4%. The automated method showed a very higher error (VME) of 16,2%, while the rapid colorimetric screening method (CRTc) showed an excellent correlation (VME 0%) with the reference method, broth microdilution.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 105-113"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Analysis of sugary kefir samples for the development of a regulatory framework in the Argentinian Food Code]. [对含糖开菲尔酒样品的分析，为阿根廷食品法典制定监管框架]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-03-24 DOI: 10.1016/j.ram.2025.02.002

Manuel Teijeiro, Analía Graciela Abraham, Josefina Cabrera, Galia Ana Suchowlanski, Ana Losada, Silvana Ruarte, Mónica López, Gabriel Vinderola

{"title":"[Analysis of sugary kefir samples for the development of a regulatory framework in the Argentinian Food Code].","authors":"Manuel Teijeiro, Analía Graciela Abraham, Josefina Cabrera, Galia Ana Suchowlanski, Ana Losada, Silvana Ruarte, Mónica López, Gabriel Vinderola","doi":"10.1016/j.ram.2025.02.002","DOIUrl":"https://doi.org/10.1016/j.ram.2025.02.002","url":null,"abstract":"<p><p>Water kefir is a fermented beverage that has experienced a growing popular interest in its production, commercialization and consumption. However, it was not contemplated as such in the Argentine Food Code (CAA, Spanish acronym). The aim of this work was to determine some microbiological and physicochemical characteristics of water kefir samples produced by Argentine entrepreneurs, to have the necessary information for the National Food Commission (CONAL, Spanish acronym) to generate an article incorporating water kefir in the CAA. For this purpose, 31 water kefir producers from different cities of the country were invited to send samples of their products for microbiological and physicochemical analysis to the National Reference Laboratory of the National Food Institute (INAL, Spanish acronym) (Buenos Aires, Argentina). In the period December 2021-February 2022, a total of 13 samples were received, together with the corresponding information on their production process. The samples analyzed showed a pH between 2.9 and 3.7, a titratable acidity between 6 and 16.6 (ml of NaOH 1N/100ml of sample), an alcohol content between 0.58 and 2.55 (%v/v), a lactic acid bacteria count of 1×10<sup>7</sup>CFU/ml and a yeast count of 1×10<sup>6</sup>CFU/ml, with enterobacteria counts lower than 10CFU/ml). The results provided local data that culminated in the publication, on August 12, 2024, of the joint resolution 7/2024 of the Secretariat of Quality in Health and Secretariat of Bioeconomy by which article 1084 tris was incorporated to the CAA in the chapter XIII of fermented beverages, referring to water kefir.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Report of a novel pathogenic orthohantavirus in Tucuman, Argentina. 阿根廷图库曼一种新型致病性正汉坦病毒报告。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2025-03-04 DOI: 10.1016/j.ram.2025.01.001

Julia Brignone, Carina Sen, María Laura Martin, Natalia Fernandez, Gabriela Delgado, Anabel Sinchi, Yael Nazar, María Cecilia Monzani, María Laura Cisneros, Patricia Clua, Rocío Coronel, Jorge García

{"title":"Report of a novel pathogenic orthohantavirus in Tucuman, Argentina.","authors":"Julia Brignone, Carina Sen, María Laura Martin, Natalia Fernandez, Gabriela Delgado, Anabel Sinchi, Yael Nazar, María Cecilia Monzani, María Laura Cisneros, Patricia Clua, Rocío Coronel, Jorge García","doi":"10.1016/j.ram.2025.01.001","DOIUrl":"https://doi.org/10.1016/j.ram.2025.01.001","url":null,"abstract":"<p><p>The confirmation of two cases of hantavirus pulmonary syndrome (HPS) in the city of San Miguel de Tucuman, province of Tucuman, is described in the present study. In 2021, the diagnosis of two patients with HPS with no history of travel outside the province was confirmed. The infection was determined by serology in both cases. Case 1 had an unfavorable outcome, was confirmed by the detection of specific IgM antibodies for orthohantavirus and the identification in a serum sample from three days of evolution of a viral genotype, which was previously unreported to be in circulation in Argentina. In Case 2, specific IgM and IgG type antibodies were detected in two serum samples, with no amplification of the viral genome. Due to the recreational habits of the patients, it is possible to infer exposure to infected rodent fluids during outdoor activities in their areas of residence. In conclusion, the circulation of a new viral genotype of orthohantavirus in Argentina is confirmed.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0