Revista Argentina de microbiologia最新文献

{"title":"Detection of Campylobacter spp. and Yersina spp. in chicken burgers: Critical evaluation of CIN medium and characterization of psychrotolerant microbiota in commercial samples from Reus (Tarragona, Spain).","authors":"Marta Sanchis, Laura Camuña-Pardo, Laia Martínez Quijada, Altea Pérez Escobar, Frederic Gómez-Bertomeu, Alberto Miguel Stchigel Glikman","doi":"10.1016/j.ram.2025.06.002","DOIUrl":"https://doi.org/10.1016/j.ram.2025.06.002","url":null,"abstract":"<p><p>Two of the three most prevalent bacterial genera associated with human diseases caused by meat consumption are Campylobacter (mainly in poultry meat) and Yersinia (in pork meat). As its detection and quantification is not regulated by the European legislation (except for the quantification of Campylobacter in poultry channels), several samples of chicken burgers from different establishments including supermarkets and retail trade outlets in Reus (Spain) were processed to ensure microbiological safety. Microbiological criteria and procedures have been those included in the European standards for Campylobacter (Regulation No. 2017/1495) and Yersinia (ISO 10273:2917). Results showed the absence of Campylobacter spp. in all samples, but high counts (10⁴ to 5×10⁵CFU/g) of typical colonies (\"red bull's eye\" morphology) on CIN medium compatible with Yersinia spp. The biochemical profile of the strains from the typical colonies, and their subsequent molecular identification using MALDI-TOF MS enabled us to identify Yersinia intermedia in just one sample, and Pseudomonas and Serratia liquefaciens in the remaining ones. These findings call into question the usefulness of CIN medium for detecting Yersinia spp. in food. Moreover, the presence of high counts of psychrotolerant bacteria from the genera Pseudomonas and Serratia highlight the need to improve hygienic conditions in the procedures used to produce meat derivatives.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and genomic surveillance of invasive Neisseria meningitidis isolates in Argentina (2015-2022): Clonal structure and vaccine implications.","authors":"Adriana Efron, Luciana Moreira, Federico Lorenzo, Denise De Belder, María Moscoloni, Alejandra Corso, Mauricio Santos, Tomás Poklepovich, Josefina Campos, Paula Gagetti, María Sol Haim","doi":"10.1016/j.ram.2025.04.005","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.005","url":null,"abstract":"<p><p>Invasive meningococcal disease is associated with high fatality rates and disabling sequelae. The aims of this study were to describe the phenotypic and genotypic characteristics of invasive Neisseria meningitidis isolates in Argentina, determine the genomic population structure and evaluate the potential coverage of vaccines targeting N. meningitidis serogroup B (MenB). During the period 2015-2022, a total of 444 isolates of N. meningitidis causing invasive disease were assessed through laboratory surveillance; 344 isolates were available for whole genome sequencing characterization. The presence of antimicrobial resistance genes, and the distribution of clonal complexes and vaccine antigens were analyzed. MenB was the most frequent serogroup (53.2%), followed by MenW (32.0%). Most isolates were susceptible to the antimicrobial agents tested; however, 56.7% exhibited intermediate resistance to penicillin. MenB showed wide genetic diversity and was mainly associated with ST-865 cc, ST-35 cc, ST-41/44 cc, and ST-32 cc. Unlike other countries, in Argentina, ST-865 cc was one of the major clonal complexes associated with MenB, MenW was only associated with ST-11 cc. To study the potential coverage of vaccines targeting MenB, we used the MenDeVAR (Meningococcal Deduced Vaccine Antigen Reactivity) index, which provides information on the presence and possible immunological cross-reactivity of the different vaccine antigenic variants. MenDeVar showed 19.3% vaccine reactivity for 4CMenB and 40.0% for the bivalent vaccine. Laboratory surveillance is essential for generating evidence-based decisions for updating vaccination strategies.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The re-emergence of vaccine-preventable diseases: The case of measles","authors":"Andrea Pontoriero ,&nbsp;Maria Laura Minassian","doi":"10.1016/j.ram.2025.07.001","DOIUrl":"10.1016/j.ram.2025.07.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 205-207"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of intestinal microbiota diversity in children with non-organic anorexia","authors":"Wei Lu,&nbsp;Zong-long Li,&nbsp;De-yong Xu,&nbsp;Guo-ping Yu","doi":"10.1016/j.ram.2025.02.001","DOIUrl":"10.1016/j.ram.2025.02.001","url":null,"abstract":"<div><div>The pathogenesis of non-organic anorexia in children is not clear. This study intends to analyze intestinal bacteria to provide a relevant theoretical basis for the clinical rational selection of microecological agents. In the present study, children with non-organic anorexia were included in the anorexia group and normal healthy children in the control group. Stool samples were collected for the bioinformatics analysis after PCR and high-throughput sequencing. The results showed that the Ace, Chao, and Shannon indexes in the anorexia group were higher than those in the control group, while the Simpson index in the control group was lower than in the anorexia group. There were 14 taxa in the anorexia group and 11 taxa in the healthy control group at the phylum level, and 193 taxa in the anorexia group and 180 in the control group at the genus level. The dominant bacteria at the phylum level of the two groups were the same, while there were 16 dominant bacteria taxa in the anorexia group and 17 in the control group at the genus level. The ratio of percentage abundance of <em>Bacteroidetes</em> to that of <em>Firmicutes</em> (the B/F index) in the anorexia group was higher than in the control group. The abundance of <em>Bacteroidetes</em> in the anorexia group was higher than that in the control group, and the abundance of <em>Actinomycetes</em> in the control group was higher than that in the anorexia group. There were significant differences in 14 dominant genera between the two groups at the genus classification level. The LEfSe multilevel species difference analysis showed that at the phylum level, the significant influential bacterial taxa in the anorexia group were <em>Bacteroidetes</em> and <em>Actinobacteria</em> in the control group. At the genus level, the significant influential bacterial taxa in the anorexia group were <em>Bacteroides</em>, <em>Faecalibacterium</em>, and <em>Subdoligranulum</em>, and <em>Bifidobacterium</em>, <em>Blautia</em>, <em>Streptococcus</em>, <em>Lachnoclostridium</em>, and <em>Erysipelatoclostridium</em> in the control group. We conclude that the increase in <em>Bacteroides</em> abundance or in the B/F index and the reduction in <em>Bifidobacterium</em> abundance were related to the pathogenesis of anorexia.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 221-230"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wheat straw amendment to an oily sludge pretreated with ammonium persulfate and its impact on the indigenous mycobiota","authors":"Natalia Andrea Di Clemente ,&nbsp;Marina Peluffo ,&nbsp;Ana Carolina Agnello ,&nbsp;Deborah Colman ,&nbsp;Norma Buceta ,&nbsp;María Teresa Del Panno ,&nbsp;Mario Carlos Nazareno Saparrat","doi":"10.1016/j.ram.2025.05.002","DOIUrl":"10.1016/j.ram.2025.05.002","url":null,"abstract":"<div><div>Numerous studies have addressed the dynamics of bacterial communities in response to remediation strategies, while fungal communities, despite their potential as bioremediation agents, remain comparatively understudied. The aim of this work was to evaluate the impact of wheat straw amendment (WSA) on an oily sludge untreated and treated with ammonium persulfate on indigenous mycobiota after 60 days. Culture-independent 18S rRNA amplicon sequencing, microbial enzymatic activity and chemical parameters were analyzed. WSA on a preoxidized oily sludge promoted high total petroleum hydrocarbon (TPH) removal after 60 days, exhibiting the highest laccase activity. Fungal diversity and equitability were also recovered until reaching similar index values to those of the untreated microcosms. However, changes in fungal taxonomical groups were detected, with Eurotiales (93.4%) being replaced by Microascales (57.9%) and Sordariales (32.8%) at the end of the treatment. Our results suggest that inputs of easily assimilable organic matter in oily sludge might accelerate changes and replacement of fungal taxa, also affecting microbial colonization and, consequently, pollutant removal. These findings highlight the relevance of incorporating fungal dynamics into bioremediation strategies as a complementary approach to oily sludge treatment.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 288-297"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic resistance to infectious diseases: A complementary tool to antimicrobial drugs in livestock?","authors":"Carlos A. Rossetti ,&nbsp;Ursula A. Rossi","doi":"10.1016/j.ram.2025.06.001","DOIUrl":"10.1016/j.ram.2025.06.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 203-204"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reporte de una experiencia de control del virus de la diarrea viral bovina en 2 tambos de Argentina aplicando herramientas de manejo, diagnóstico y vacunación","authors":"Rodrigo Pereyra ,&nbsp;Fernando Martino ,&nbsp;Mariángeles Castillo ,&nbsp;Juan Manuel Sala ,&nbsp;Lucas José Barone ,&nbsp;Claudio Paolazzi ,&nbsp;Alejandra Victoria Capozzo","doi":"10.1016/j.ram.2024.12.001","DOIUrl":"10.1016/j.ram.2024.12.001","url":null,"abstract":"<div><div>Bovine viral diarrhea virus (BVDV) causes significant economic losses in the international livestock industry and in Argentina, where it circulates at high prevalence. Under high prevalence conditions, BVDV infections are controlled through vaccination once persistently infected animals are identified and segregated. This study evaluated the feasibility of controlling BVDV circulation under field conditions by combining diagnosis, management measures, and vaccination in 2 dairy farms in the province of Santa Fe. Commercial ELISAs were used for the detection of the NS3 (P80) protein or antibodies against this protein as well as an RT-nested PCR for the detection of the viral genome, and viral seroneutralization to assess vaccine efficacy. The average seroprevalence of the farms was 58.4%, with a persistently infected animal rate of 2.4%. After segregating the persistently infected animals and vaccinating them with a commercial combined vaccine containing inactivated BVDV, abortion rates significantly decreased (<em>p</em> <!-->&lt;<!--> <!-->0.05) in farm 1 (from 20.5 to 11.6%) and in farm 2 (from 34 to 23.4%) during the second year of the control strategy. Conception rates increased from 29 to 33% in farm 1 during the first year, while in farm 2, the increase was 7 points during the second year. This methodology achieved conditions in which BVDV ceased to circulate, constituting the first controlled report on BVDV management in dairy farms using tools available to producers in Argentina.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 241-250"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and demographic characteristics of spondylodiscitis in a Mexican population: A retrospective study","authors":"Mario Alberto Dueñas-Espinosa ,&nbsp;Julio César López-Valdés ,&nbsp;Daniel Alejandro Vega-Moreno ,&nbsp;María Fernanda Guzmán-del Río ,&nbsp;Rafael Sánchez-Mata ,&nbsp;María Elena Córdoba-Mosqueda ,&nbsp;Laura Mestre-Orozco ,&nbsp;Erick Alberto Castañeda-Ramírez ,&nbsp;Alejandro Jacob Madrid-Sánchez ,&nbsp;Ulises García-González","doi":"10.1016/j.ram.2025.01.002","DOIUrl":"10.1016/j.ram.2025.01.002","url":null,"abstract":"<div><div>The aim of this study was to identify the clinical and demographic characteristics of patients with a confirmed diagnosis of spondylodiscitis through microbiological cultures. A descriptive, observational, and retrospective study was conducted. Patients were included based on clinical and radiological evidence of vertebral infection, unspecified discitis, and/or positive microbiological cultures consistent with spondylodiscitis. For the comparison between men and women, the Student's <em>t</em>-test and odds ratio were employed. The Chi-square test was used to examine correlations between affected spinal levels, isolated microorganisms, and associated comorbidities. A total of 86 cases of discitis were identified, 65% of which involved male patients. The mean age was 59.0<!--> <!-->±<!--> <!-->11.5 years (range: 38–83), and the average body mass index (BMI) was 28<!--> <!-->±<!--> <!-->4.05<!--> <!-->kg/m². Primary discitis predominated in 68% of cases, mainly at the thoracic level. Seventeen patients presented with spondylodiscitis not associated with chronic degenerative diseases. The most frequently isolated microorganisms were <em>Staphylococcus aureus</em> (28 cases) and <em>Escherichia coli</em> (21 cases). In 16 cases, intracellular pathogens such as <em>Mycobacterium tuberculosis</em> and <em>Brucella</em> spp. were identified, leading to an average hospital stay of 30 days. Spondylodiscitis is a serious complication, and this study highlights differences from previously published data, particularly in terms of the microorganisms involved and the demographic profile of the population.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 256-264"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial diversity investigation using 16S metagenomics in Tunisian patients with systemic lupus erythematosus","authors":"Amira Messadi ,&nbsp;Sameh Sayhi ,&nbsp;Kais Ghedira ,&nbsp;Chadia Zaouaoui ,&nbsp;Bilel Arfaoui ,&nbsp;Soumaya Khouikhi ,&nbsp;Maha Rebai ,&nbsp;Nour El Houda Guediche ,&nbsp;Nadia Abdelhafidh ,&nbsp;Bassem Louzir ,&nbsp;Ajili Faida","doi":"10.1016/j.ram.2025.04.002","DOIUrl":"10.1016/j.ram.2025.04.002","url":null,"abstract":"<div><div>Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease associated with significant morbidity and mortality. It is characterized by a loss of self-immune tolerance and autoantibody production, leading to multiple organ damage. Emerging investigations have confirmed the role of gut microbiota dysbiosis in patients with SLE, although the underlying mechanisms remain unclear to date. In this study, we aim to investigate the bacterial profile of SLE including phylum/class/genus relative abundance and diversity, to compare them with healthy controls and to study the correlation of relative abundance of different patterns with clinical/biological parameters. In this case–control study, the bacterial profile was investigated in 7 SLE patients and 7 healthy controls using 16S metagenomics clustering. The present study reported a low abundance of the class Bacilli (0.58% in SLE vs 1.26% in the controls), the genus <em>Lactobacillus</em> (0.43% vs 0.74%), as well as a higher abundance of the genera <em>Gammaproteobacteria</em> (2.37% vs 0.77%) and <em>Escherichia</em>–<em>Shigella</em> (2.04% vs 0.51%) in SLE samples compared to the controls (<em>p</em> <!-->&lt;<!--> <!-->0.05). We also found an association between the class Betaproteobacteria (4.42% vs 1.57%) and the genus <em>Faecalibacterium</em> (11.34% vs 3.35%) and renal manifestations (<em>p</em> <!-->&lt;<!--> <!-->0.05). The phylum Actinobacteria (0.21% vs 3.8%, <em>p</em> <!-->=<!--> <!-->0.036) and the genus <em>Bifidobacterium</em> levels were lower in active SLE compared to the healthy controls. This study is the first report on the gut microbiota of SLE and the first case–control study in Tunisia and North Africa. We obtained a particular profile of bacterial gut microbiota for the SLE group. We found a specific clustering when compared to the healthy controls.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 275-287"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Análisis de muestras de kéfir de agua para el desarrollo de un marco regulatorio en el Código Alimentario Argentino","authors":"Manuel Teijeiro ,&nbsp;Analía Graciela Abraham ,&nbsp;Josefina Cabrera ,&nbsp;Galia Ana Suchowlanski ,&nbsp;Ana Losada ,&nbsp;Silvana Ruarte ,&nbsp;Mónica López ,&nbsp;Gabriel Vinderola","doi":"10.1016/j.ram.2025.02.002","DOIUrl":"10.1016/j.ram.2025.02.002","url":null,"abstract":"<div><div>Water kefir is a fermented beverage that has experienced a growing popular interest in its production, commercialization and consumption. However, it was not contemplated as such in the Argentine Food Code (CAA, Spanish acronym). The aim of this work was to determine some microbiological and physicochemical characteristics of water kefir samples produced by Argentine entrepreneurs, to have the necessary information for the National Food Commission (CONAL, Spanish acronym) to generate an article incorporating water kefir in the CAA. For this purpose, 31 water kefir producers from different cities of the country were invited to send samples of their products for microbiological and physicochemical analysis to the National Reference Laboratory of the National Food Institute (INAL, Spanish acronym) (Buenos Aires, Argentina). In the period December 2021-February 2022, a total of 13 samples were received, together with the corresponding information on their production process. The samples analyzed showed a pH between 2.9 and 3.7, a titratable acidity between 6 and 16.6 (ml of NaOH 1N/100<!--> <!-->ml of sample), an alcohol content between 0.58 and 2.55 (%v/v), a lactic acid bacteria count of 1<!--> <!-->×<!--> <!-->10⁷ <!-->CFU/ml and a yeast count of 1<!--> <!-->×<!--> <!-->10⁶ <!-->CFU/ml, with enterobacteria counts lower than 10<!--> <!-->CFU/ml). The results provided local data that culminated in the publication, on August 12, 2024, of the joint resolution 7/2024 of the Secretariat of Quality in Health and Secretariat of Bioeconomy by which article 1084 tris was incorporated to the CAA in the chapter XIII of fermented beverages, referring to water kefir.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 298-303"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}