Revista Argentina de microbiologia最新文献_第5页

Low-cost antiseptic-impregnated tracheostomy tube for the prevention of ventilator-associated pneumonia caused by multidrug-resistant bacteria: In vitro and pilot study in humans 用于预防耐多药细菌引起的呼吸机相关肺炎的低成本消毒浸渍气管造口管：体外和人体试验研究。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.05.004

Juliano Gasparetto , Leandro Bressianini Jurkonis , Leticia Ramos Dantas , Paula Hansen Suss , Felipe Francisco Tuon

引用次数: 0

Mycobacterium tuberculosis Beijing in the State of Nuevo Leon, Mexico 墨西哥新莱昂州的北京结核分枝杆菌。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2023.12.007

Nohemí Catana-Botello , Pola Becerril-Montes , Jorge Castro-Garza , Francisco González-Salazar , Horacio Almanza-Reyes , María De Los Ángeles Del Bosque-Moncayo , Alejandro Morales-Vargas , Víctor Manuel Velázquez-Moreno

{"title":"Mycobacterium tuberculosis Beijing in the State of Nuevo Leon, Mexico","authors":"Nohemí Catana-Botello , Pola Becerril-Montes , Jorge Castro-Garza , Francisco González-Salazar , Horacio Almanza-Reyes , María De Los Ángeles Del Bosque-Moncayo , Alejandro Morales-Vargas , Víctor Manuel Velázquez-Moreno","doi":"10.1016/j.ram.2023.12.007","DOIUrl":"10.1016/j.ram.2023.12.007","url":null,"abstract":"<div><div>Tuberculosis remains a serious threat to human health as an infectious disease in Mexico. Data about the genotypes of circulating <em>Mycobacterium tuberculosis</em> isolates (MTB) in the State of Nuevo Leon, Mexico are scarce. We aimed to determine the genotypes of circulating MTB belonging to the Beijing lineage recovered from patients in the State of Nuevo Leon, Mexico. A total of 406 MTB isolates from this state were genotyped using the spoligotyping method and 18-locus MIRU-VNTR. Lineage classification and MTB transmission analysis were performed. Based on the spoligotyping analysis, we found 24 strains belonging to the Beijing genotype that were characterized phylogenetically. The MIRUs showed greater discriminatory power than the standard RFLP-IS6110 method; therefore, the greatest allelic diversity among the Beijing strains was observed with MIRU10, MIRU31, MIRU39, MRU40, and MIRU 26. MVLA analysis showed a profile variation between Beijing and non-Beijing strains. The minimum spanning tree (MST) showed that 79% (19) of the strains are related. All Beijing strains exhibited the deletion of region TbD1, which is a characteristic of modern strains. The application of spoligotyping and MIRU-VNTR-18 methods together proved to be more sensitive, discriminatory, and rapid than the standard method for the epidemiological analysis of <em>Mycobacterium Beijing</em> isolates. This study is one of the first to describe the genomic diversity of <em>M. Beijing</em> in the State of Nuevo Leon, Mexico.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 380-389"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Norovirus outbreaks in a nursery school in Buenos Aires, Argentina 阿根廷布宜诺斯艾利斯一家幼儿园爆发诺如病毒。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.07.001

Karina A. Gomes, Juan I. Degiuseppe, Juan A. Stupka

引用次数: 0

Streptococcus spp. isolated from bovine mastitis: Antimicrobial sensitivity studies and disagreement evaluation between routine phenotypic diagnosis and molecular identification 从牛乳腺炎中分离出的链球菌：抗菌药敏感性研究以及常规表型诊断与分子鉴定之间的分歧评估。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.07.006

Leticia Diana , Lucas Mastroianni , Virginia Diana , Rodrigo Puentes

{"title":"Streptococcus spp. isolated from bovine mastitis: Antimicrobial sensitivity studies and disagreement evaluation between routine phenotypic diagnosis and molecular identification","authors":"Leticia Diana , Lucas Mastroianni , Virginia Diana , Rodrigo Puentes","doi":"10.1016/j.ram.2024.07.006","DOIUrl":"10.1016/j.ram.2024.07.006","url":null,"abstract":"<div><div>Bovine mastitis poses a significant threat to global dairy production, resulting in substantial losses in milk production. <em>Streptococcus</em> bacteria, particularly <em>Streptococcus uberis</em>, <em>Streptococcus agalactiae</em>, and <em>Streptococcus dysgalactiae</em>, are commonly implicated in this condition. An accurate diagnosis is crucial for implementing effective treatment and minimizing its impact on production. This study examined 115 <em>Streptococcus</em> strains isolated from bovine mastitis cases in Uruguay using PCR for species identification. Additionally, the resistance to tetracycline, erythromycin, and penicillin was assessed in 81 of the bacterial strains under study. Significant disparities between phenotypic and genotypic detection were evident across all three species, with only 31% of strains identified phenotypically aligning with PCR results. Phenotypic prevalence indicated <em>S. dysgalactiae</em> as the most prevalent (44.35%), followed by <em>S. uberis</em> (24.34%) and <em>S. agalactiae</em> (6.09%). However, the genotypic identification revealed <em>S. uberis</em> as the most prevalent, followed by <em>S. dysgalactiae</em>, while <em>S. agalactiae</em> remained the least prevalent. The high sensitivity and speed of PCR suggest its potential routine implementation for diagnosing bovine mastitis caused by <em>Streptococcus</em> in any laboratory. Although, penicillin resistance was practically nonexistent, tetracycline and erythromycin exhibit higher resistance levels across all three species studied. In conclusion, the study underlines the importance of early diagnosis, highlights variations in bacterial prevalence, and proposes PCR as a valuable diagnostic tool for <em>Streptococcus</em> species responsible for bovine mastitis.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 351-358"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutaneous infection in an immunocompromised domestic cat caused by Setosphaeria turcica (Luttr.) Leonard & Suggs 由Setosphaeria turcica (Luttr.) Leonard & Suggs引起的一只免疫力低下家猫的皮肤感染。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.08.003

Fábio Antônio Antonelo , Mariana Soares Rodrigues , Thiago Jean Kraus , Mayara Souza da Silva , Paula Fernandes Montanher

引用次数: 0

Chagas disease and immunosuppressive therapy: When therapeutic interventions can harm 恰加斯病和免疫抑制疗法：当治疗干预可能有害。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.11.004

Catalina D. Alba Soto , Silvia A. Repetto

引用次数: 0

Anaerobic digestates in agricultural soils: A systematic review of their effects on antibiotic resistance genes 农业土壤中的厌氧消化物：系统回顾其对抗生素抗性基因的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.07.005

Marco Allegrini , María Celina Zabaloy

{"title":"Anaerobic digestates in agricultural soils: A systematic review of their effects on antibiotic resistance genes","authors":"Marco Allegrini , María Celina Zabaloy","doi":"10.1016/j.ram.2024.07.005","DOIUrl":"10.1016/j.ram.2024.07.005","url":null,"abstract":"<div><div>Tackling the dissemination of antibiotic resistance is one of the main global challenges. Manures from animal production are a recognized source of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) requiring appropriate treatment methods. One of the main approaches for manure treatment is anaerobic digestion (AD). Meta-analyses have demonstrated that AD can significantly reduce the load of ARGs. However, antibiotics, ARGs and MGEs still remain in the final product (digestate). A sustainable agricultural use of digestates under the One Health framework requires wide assessments of their effects in the soil resistome. The objective of this review was to present the state of the art of digestate effects on ARGs of agricultural soils, focusing exclusively on digestates from animal manures. A systematic review was conducted. The examination of the resulting literature indicated that although temporal decays are observed for a variety of ARGs in single-application and repeated-applications experiments, for certain ARGs the pre-treatment or control levels are not restored. However, the low number of studies and the heterogeneous experimental conditions preclude a clear understanding of the fate of ARGs in soil and their risk for agroecosystems. The inclusion of multiple MGEs and the assessment of the long-term influence of digestates on soil properties and microbial communities could be keystones for a better understanding of the risks associated with digestate-induced changes in the soil resistome.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 394-401"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigación del perfil de resistencia a betalactámicos en cepas clínicas de Pseudomonas aeruginosa en Mexicali, 2019-2021 [调查墨西卡利铜绿假单胞菌菌株对 Beta-内酰胺类药物的耐药性概况：2019-2021 年]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.10.002

Ricardo Delgadillo-Valles , Dolores A. Marquez-Salazar , Donato A. Rechy-Iruretagoyena , Gerson N. Hernandez-Acevedo , Jonathan I. Arauz-Cabrera , Edwin Barrios-Villa

{"title":"Investigación del perfil de resistencia a betalactámicos en cepas clínicas de Pseudomonas aeruginosa en Mexicali, 2019-2021","authors":"Ricardo Delgadillo-Valles , Dolores A. Marquez-Salazar , Donato A. Rechy-Iruretagoyena , Gerson N. Hernandez-Acevedo , Jonathan I. Arauz-Cabrera , Edwin Barrios-Villa","doi":"10.1016/j.ram.2024.10.002","DOIUrl":"10.1016/j.ram.2024.10.002","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> is a Gram-negative bacillus capable of developing in humid environments and animal tissue. The interest in this bacterium lies in its ability to cause opportunistic diseases in patients with cystic fibrosis and healthcare-associated infections (HAIs). The objective of our study was to characterize the resistance profile of strains causing HAIs isolated in hospitals within our community, from January 2019 to December 2021. This descriptive, prospective, and cross-sectional study involved the isolation of strains from January 2019 to December 2021 at the Autonomous University of Baja California (UABC). The identification of the strains was carried out using Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry, and the detection of beta-lactam resistance was performed according to the criteria of the Clinical and Laboratory Standards Institute as stipulated in the CLSI M100-S27 document. A total of 649 samples were obtained from January 2019 to December 2021, including sputum (335 samples), urine (119 samples), and wounds (91 samples). Resistance to carbapenems was 38.94% for meropenem and 21.97% for imipenem. For cephalosporins, there was a 21.05% resistance rate for cefepime, 22.9% for ceftazidime, and 24.78% for ceftolozane-tazobactam. The prevalence of antimicrobial resistance has increased over time, which is attributable to both selective pressure and the evolution of the microorganisms themselves.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 368-372"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incidencia de microorganismos deteriorantes de la calidad de cerveza en microcervecerías de Buenos Aires [布宜诺斯艾利斯微型啤酒厂啤酒腐败微生物的发生率]。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.09.006

Juan I. Eizaguirre , Clara Bruzone , Ignacio Duhourq , Diego Libkind , Pablo S. Aguilar

{"title":"Incidencia de microorganismos deteriorantes de la calidad de cerveza en microcervecerías de Buenos Aires","authors":"Juan I. Eizaguirre , Clara Bruzone , Ignacio Duhourq , Diego Libkind , Pablo S. Aguilar","doi":"10.1016/j.ram.2024.09.006","DOIUrl":"10.1016/j.ram.2024.09.006","url":null,"abstract":"<div><div>Microbial contaminations pose a significant concern within the brewing industry, exerting negative effects on the organoleptic quality of the product and leading to substantial economic losses. The exponential proliferation of craft breweries in Argentina in recent years has heightened the demand for constant improvements in processes to ensure excellence in beer production. However, the stringency of microbiological quality controls remains a vulnerable area. This study assesses the prevalence of beer contaminants in samples from 10 breweries located in Buenos Aires City (CABA) and Greater Buenos Aires area (GBA). The results revealed the presence of microorganisms in 70% of the analyzed samples. Fifteen bacteria and 19 yeasts were successfully isolated, with bacteria belonging to the genera <em>Acetobacter</em> and <em>Staphylococcus</em>, and yeasts to the genera <em>Saccharomyces</em>, <em>Lodderomyces</em>, <em>Candida</em>, and <em>Pichia</em>. Accurately identifying these microorganisms provides producers with the necessary information for formulating action plans to improve cleaning and sanitization protocols in their facilities. This proactive approach not only has the potential to mitigate economic losses associated with microbial contamination but also contributes to maintaining and elevating quality standards in regional craft beer production.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 402-412"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall structure and its influence on aflatoxin B₁ binding. Kluyveromyces marxianus VM004 培养条件对细胞壁结构的影响及其对黄曲霉毒素 B1 结合力的影响。

IF 1.8 4区生物学

Revista Argentina de microbiologia Pub Date : 2024-09-20 DOI: 10.1016/j.ram.2024.07.004

Carina Pereyra, María Del Pilar Monge, Silvestre Bongiovanni, Andrea Cristofolini, Sergio Campos, Lilia Cavaglieri

{"title":"Impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall structure and its influence on aflatoxin B<sub>1</sub> binding.","authors":"Carina Pereyra, María Del Pilar Monge, Silvestre Bongiovanni, Andrea Cristofolini, Sergio Campos, Lilia Cavaglieri","doi":"10.1016/j.ram.2024.07.004","DOIUrl":"https://doi.org/10.1016/j.ram.2024.07.004","url":null,"abstract":"<p><p>The aim of this study was to determine the impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall (CW) structure and its influence on aflatoxin B<sub>1</sub> binding. The yeast was inoculated into two types of culture media: yeast extract-peptone-dextrose (YPD) broth and dried distiller's grains with solubles (DDG). The CW was extracted from the biomass produced in these media. AFB<sub>1</sub> (150ng/ml) adsorption tests using the biomass (1×10<sup>7</sup>cells/ml) and the CW (0.001g) were performed at pH 2 and pH 8. Transmission electron microscopy (TEM) evaluated the CW thickness, and infrared spectroscopy (IR) determined the CW composition. Biomass production in YPD was higher than that in DDG. Cell diameter (μm) and CW thickness (μm) increased in the DDG medium. The CW percentage obtained in DDG was higher than that in YPD. The absorbance of carbohydrates by IR was higher in YPD. pH influenced AFB<sub>1</sub> adsorption, which was lower at pH 8. The proportion of β-glucan and chitin present in CW was higher in the YPD medium. The IR method allowed to study the CW carbohydrate variation under the influence of these carbon sources. In conclusion, the culture media composition influenced the β-glucan and chitin composition and consequently, AFB<sub>1</sub> adsorption.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0