María Dolores Rojo-Martín , Juan Diego Ruiz-Mesa , Concepción Mediavilla-Gradolph , Mercedes Marín-Arriaza , Alexandra Halbardier-Carretero , José Serrano-Sánchez , Ignacio Márquez-Gómez , Inmaculada de Toro-Peinado , Luis Francisco Caballero-Martínez , Aurora García-Barrionuevo , Begoña Palop-Borrás
{"title":"Impacto de los marcadores microbiológicos en la evolución de la infección por Clostridioides difficile","authors":"María Dolores Rojo-Martín , Juan Diego Ruiz-Mesa , Concepción Mediavilla-Gradolph , Mercedes Marín-Arriaza , Alexandra Halbardier-Carretero , José Serrano-Sánchez , Ignacio Márquez-Gómez , Inmaculada de Toro-Peinado , Luis Francisco Caballero-Martínez , Aurora García-Barrionuevo , Begoña Palop-Borrás","doi":"10.1016/j.ram.2024.12.003","DOIUrl":"10.1016/j.ram.2024.12.003","url":null,"abstract":"<div><div>The main current diagnostic challenge in <em>Clostridioides difficile</em> infection (CDI) is to detect patients with the poorest prognoses. We have studied the association between the presence of toxin in the feces, the amplification of the toxin B gene at low cycles in PCR (Ct), and the detection of the binary toxin or ribotype with severity, recurrence, and poor prognosis. Toxigenic <em>C. difficile</em> has been investigated in adult diarrheal feces, regardless of the clinical request. Glutamate dehydrogenase (GDH), toxin A (TcdA) and toxin B (TcdB) have been assessed by immunoassay and the gene encoding toxin B (tcdB) and the binary toxin (cdtA) by PCR. The toxigenic strains recovered by toxigenic culture were typed by PCR-Ribotyping and subtyped by MLVA (multilocus-variable number tandem DNA repeat analysis). Eighty-two (82) cases of CDI were detected from 677 samples (12.1%). Patients with Ct ≤ 27.5 often presented criteria for severity, recurrence, and poor prognosis (59.3% versus 40.7%, <em>P</em>=.15; 80% versus 20%, <em>P</em>=.10; and 60.3% versus 39.7%, <em>P</em>=.055, respectively). The mean Ct was lower when recurrence was observed (25.48; SD<!--> <!-->=<!--> <!-->2.41; <em>P</em>=.058). By logistic regression, the variable most likely to indicate a poor prognosis was Ct ≤ 27.5 (OR<!--> <!-->=<!--> <!-->2.663; 95% CI=.983-7.636; <em>P</em>=.059). In summary, the cycle threshold of the PCR for toxin B is a possible marker of severity, recurrence, and poor prognosis in CDI. The diversity of ribotypes observed is remarkable, with no clonal relationship between isolates of the same ribotype.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 142-151"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thelma J. Parra-Aguilar, María de Jesús Yáñez-Morales, Alejandra Almaraz-Sánchez, Alfonsina Judith Hernández, Sergio Aranda-Ocampo
{"title":"Gluconobacter spp. y Paenibacillus polymyxa causan pudrición en zanahorias comerciales aparentemente sanas","authors":"Thelma J. Parra-Aguilar, María de Jesús Yáñez-Morales, Alejandra Almaraz-Sánchez, Alfonsina Judith Hernández, Sergio Aranda-Ocampo","doi":"10.1016/j.ram.2024.12.009","DOIUrl":"10.1016/j.ram.2024.12.009","url":null,"abstract":"<div><div>Postharvest diseases of carrots are one of the most important concerns in carrot storage, as they affect the shelf life of carrots and cause significant financial losses. In this study, ten commercial carrot samples from supermarkets and local markets in Texcoco, State of Mexico, Mexico, were analyzed for bacterial spoilage after incubation at 28<!--> <!-->°C in a humidity chamber. Carrots from seven samples developed bacterial spoilage after five days of incubation, of which baby carrots (industrially processed) showed the most severe rot. Twenty-three bacterial strains were isolated from the tissues of spoiled carrots. To identify and characterize the spoilage pathogens, the isolated strains were inoculated into healthy carrots in laboratory tests. Of the 23 strains analyzed, eight caused carrot tissue spoilage. Biochemical and molecular characterization by 16S ribosomal RNA gene sequencing identified <em>Gluconobacter cerinus</em> (1032.2, 1059 and 1070.1), <em>G.</em> <em>kondonii</em> (1027.1), <em>G.</em> <em>wancherniae</em> (1033.1) and <em>Paenibacillus polymyxa</em> (1074.2, 1076 and 1077) as the causal agents of the carrot rot evaluated in this study. In addition, these bacteria showed virulence in other plant pathogenicity tests; <em>Gluconobacter</em> strains induced a hypersensitivity reaction in tobacco leaves and <em>Paenibacillus</em> strains showed pectolytic activity in potato tubers. This study is the first to report <em>G.</em> <em>cerinus</em>, <em>G.</em> <em>kondonii</em>, <em>G.</em> <em>wancherniae</em> and <em>P.</em> <em>polymyxa</em> as causal agents of commercial carrot rot.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 192-201"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The dual impact of Trichoderma on soil microbial communities: beneficial or disruptive?","authors":"Adriana M. Torres","doi":"10.1016/j.ram.2025.04.003","DOIUrl":"10.1016/j.ram.2025.04.003","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 87-88"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nicolás Cimmino , Maria Josefina Etchevers , Astrid Smud , Patricia Yolleth Andrade Pino , Marisa del Lujan Sanchez , Maria Laura Gonzalez , Maria Victoria Acosta , Gissel Zuñiga , Karina Alejandra Perez , Juana Freggiaro , Valeria Rosa Alexander , Maria Florencia Veiga , Mariangeles Visus , Mariano Martin Marcolongo
{"title":"Use of stool molecular panel in hospitalized patients with diarrhea. Experience in a tertiary care center","authors":"Nicolás Cimmino , Maria Josefina Etchevers , Astrid Smud , Patricia Yolleth Andrade Pino , Marisa del Lujan Sanchez , Maria Laura Gonzalez , Maria Victoria Acosta , Gissel Zuñiga , Karina Alejandra Perez , Juana Freggiaro , Valeria Rosa Alexander , Maria Florencia Veiga , Mariangeles Visus , Mariano Martin Marcolongo","doi":"10.1016/j.ram.2024.10.005","DOIUrl":"10.1016/j.ram.2024.10.005","url":null,"abstract":"<div><div>Infectious diarrhea is a common health issue that affects a large number of individuals each year. It causes significant morbidity and mortality, greatly impacting healthcare system costs. Rapid detection of the causative organism and timely treatment alters the management and outcome of the condition. Molecular panels in stool allow to analyze a wide range of pathogens quickly and easily. For this study, a cross-sectional cohort analysis with a retrospective analysis of adult patients hospitalized with diarrhea and negative conventional stool bacteriological studies was conducted. Data obtained from the use of molecular panels in stool and the role of endoscopy in the diagnostic pathway were analyzed. A positivity rate of 52% (n<!--> <!-->=<!--> <!-->41) out of a total of 79 samples was reported. The test contributed to a change in therapeutic approach in 58% (n<!--> <!-->=<!--> <!-->46) of the patients. Among the patients with a negative molecular panel, 39.5% underwent further evaluation with colonoscopy involving biopsies, resulting in a diagnostic yield of 87%. Based on these results, we can conclude that molecular techniques contribute to the diagnosis and change in therapeutic approach in hospitalized patients with diarrhea.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 98-104"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raquel Jara , Dafne Santos , Noelia Soledad Reyes , Eliana Hermida , Alejandro Seoane , Martin Ypas , Gisela Andres , Marcela Echavarría
{"title":"Endemic coronavirus in children and adults with acute respiratory infection before the COVID-19 pandemic","authors":"Raquel Jara , Dafne Santos , Noelia Soledad Reyes , Eliana Hermida , Alejandro Seoane , Martin Ypas , Gisela Andres , Marcela Echavarría","doi":"10.1016/j.ram.2024.11.003","DOIUrl":"10.1016/j.ram.2024.11.003","url":null,"abstract":"<div><div>Acute respiratory infection (ARI) is one of the principal causes of morbidity worldwide, with respiratory viruses being common etiological agents. Among them, endemic human coronaviruses (hCoVs) including CoV-229E, CoV-OC43, CoV-NL63, and CoV-HKU1 can cause mild ARI but are usually not evaluated in the clinical setting. The aim of this work was to determine the prevalence of all respiratory pathogens, with the focus placed on endemic hCoVs in the pre-pandemic period. Circulating species, clinical associations and coinfections with other respiratory pathogens were evaluated in 510 immunocompetent patients (children and adults) with ARI using the FilmArray® Respiratory Panel (BioFire/bioMérieux). A total of 399 children (252 outpatients and 147 hospitalized) and 111 adult outpatients were enrolled in the pre-pandemic period (2008–2010 and 2016). Endemic hCoVs were the third and fifth more frequently detected viruses among adults and outpatient children, respectively, with an overall frequency close to 10%. The most prevalent species were CoV-OC43 (42.8%) and CoV-HKU1 (40.5%), followed by CoV-NL63 (19.0%) and CoV-229E (4.8%). Tachypnea, wheezing and chest indrawing were more frequent in hospitalized children compared to outpatients. All adult patients presented with symptoms of a common cold. Endemic hCoVs were detected year-round, primarily between June and November. Our results highlight their clinical relevance, and the need to include endemic hCoVs in routine screening. In the post-pandemic period, further long-term surveillance is needed for understanding the epidemiology of endemic hCoVs and their evolution, as a tool to anticipate the possible emergence of new species.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 161-170"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eduardo Montalvo , Florencia Veiga , Hernán Rodríguez , German Traglia , Carlos Vay , Marisa Almuzara
{"title":"Identificación y sensibilidad antibiótica de aislamientos de Aeromonas spp. en un Hospital Universitario de la ciudad de Buenos Aires","authors":"Eduardo Montalvo , Florencia Veiga , Hernán Rodríguez , German Traglia , Carlos Vay , Marisa Almuzara","doi":"10.1016/j.ram.2024.11.001","DOIUrl":"10.1016/j.ram.2024.11.001","url":null,"abstract":"<div><div><em>Aeromonas</em> spp. are opportunistic pathogens that cause both intra- and extraintestinal infections. The objective of this work was the phenotypic and genotypic characterization of a collection of <em>Aeromonas</em> strains, in addition to determining their sensitivity to different antimicrobials. Thirty seven isolates were analyzed. 54% were of intra-abdominal origin, 22% from skin and soft tissues, 19% from the bloodstream, among other less frequent sites. By amplification and sequencing of the <em>gyrB</em> gene, which was considered the reference method, the following were identified: 37,8% as species of the <em>Aeromonas hydrophila</em> complex, 32,4% as species of the <em>Aeromonas veronii</em> complex, and 29,7% as species of the complex <em>Aeromonas caviae</em>. Identification by traditional biochemical tests presented a better correlation with molecular identification than mass spectrometry (MALDI TOF MS). Regarding antibiotic sensitivity, cefotaxime, ceftazidime, cefepime, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, ciprofloxacin, amikacin, gentamicin and nitrofurantoin showed activity on more than 80.0% of the isolates tested. The sensitivity and specificity of the phenotypic methods to determine the presence of carbapenemases in relation to the detection of the cphAgene, the reference method, was 60,9% and 100%, respectively, for the colorimetric assay (Blue Carba), and of 91,3% and 50,0% respectively, for the modified Hodge test. The overall resistance to colistin was 32,4%. The automated method showed a very higher error (VME) of 16,2%, while the rapid colorimetric screening method (CRTc) showed an excellent correlation (VME 0%) with the reference method, broth microdilution.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 105-113"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Julia Brignone, Carina Sen, María Laura Martin, Natalia Fernandez, Gabriela Delgado, Anabel Sinchi, Yael Nazar, María Cecilia Monzani, María Laura Cisneros, Patricia Clua, Rocío Coronel, Jorge García
{"title":"Report of a novel pathogenic orthohantavirus in Tucuman, Argentina.","authors":"Julia Brignone, Carina Sen, María Laura Martin, Natalia Fernandez, Gabriela Delgado, Anabel Sinchi, Yael Nazar, María Cecilia Monzani, María Laura Cisneros, Patricia Clua, Rocío Coronel, Jorge García","doi":"10.1016/j.ram.2025.01.001","DOIUrl":"https://doi.org/10.1016/j.ram.2025.01.001","url":null,"abstract":"<p><p>The confirmation of two cases of hantavirus pulmonary syndrome (HPS) in the city of San Miguel de Tucuman, province of Tucuman, is described in the present study. In 2021, the diagnosis of two patients with HPS with no history of travel outside the province was confirmed. The infection was determined by serology in both cases. Case 1 had an unfavorable outcome, was confirmed by the detection of specific IgM antibodies for orthohantavirus and the identification in a serum sample from three days of evolution of a viral genotype, which was previously unreported to be in circulation in Argentina. In Case 2, specific IgM and IgG type antibodies were detected in two serum samples, with no amplification of the viral genome. Due to the recreational habits of the patients, it is possible to infer exposure to infected rodent fluids during outdoor activities in their areas of residence. In conclusion, the circulation of a new viral genotype of orthohantavirus in Argentina is confirmed.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan Leandro Pellegrini, María de Los Ángeles González, Liliana Silvina Lösch, Luis Antonio Merino, José Alejandro Di Conza
{"title":"Colistin-resistant Escherichia coli mediated by the mcr-1 gene from pigs in northeastern Argentina.","authors":"Juan Leandro Pellegrini, María de Los Ángeles González, Liliana Silvina Lösch, Luis Antonio Merino, José Alejandro Di Conza","doi":"10.1016/j.ram.2024.12.013","DOIUrl":"https://doi.org/10.1016/j.ram.2024.12.013","url":null,"abstract":"<p><p>The emergence and spread of multidrug-resistant Escherichia coli carrying mcr-1 is recognized as a threat to public health. The aim of this study was to determine the prevalence of the mcr-1 gene in colistin-resistant E. coli isolates from commercial pig farms in Chaco, Argentina from 2020 to 2021. A total of 140 rectal swab samples were collected from pigs in six different pig production farms. Antimicrobial susceptibility was determined by broth microdilution. mcr-1 to mcr-5 genes were identified by multiplex PCR and clonality was assessed by ERIC and REP-PCR. The prevalence of mcr-1 was 16.4% and mcr-2, mcr-3, mcr-4 and mcr-5 genes were not detected. Colistin MIC values showed a bimodal distribution with a MIC50, MIC90 and a range of 4, 8 and 4-8μg/ml, respectively. The resistance profile to other antimicrobials was: ampicillin, 87% (20); ampicillin-sulbactam, 47.8% (11); amoxicillin-clavulanic, 13% (3); chloramphenicol, 82.6% (19); ciprofloxacin, 60.9% (14); minocycline, 26.1% (5) and trimethoprim/sulfamethoxazole, 43.5% (10). Eighty-seven percent (87%) of the strains were categorized as MDR and 12 phenotypic resistance patterns with different clonality profiles were observed. A high prevalence of mcr-1 is demonstrated in colistin-free pig farms from Chaco, Argentina. The mcr-1 positive E. coli isolates showed an alarming level of multidrug resistance and high clonal diversity. It is necessary to continuously monitor the presence of the mcr-1 gene not only in pig production, but also in humans and the environment.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Silvana Ramadán, Lucía Bulacio, Hernán Dalmaso, Graciela Sepúlveda, Maximiliano Sortino, Fabián Fay, Claudia Misto, María Fernanda Salvador, Alejandro Etchecopaz, María Luján Cuestas
{"title":"First report of canine protothecosis caused by Prototheca wickerhamii in Argentina. Brief literature review.","authors":"Silvana Ramadán, Lucía Bulacio, Hernán Dalmaso, Graciela Sepúlveda, Maximiliano Sortino, Fabián Fay, Claudia Misto, María Fernanda Salvador, Alejandro Etchecopaz, María Luján Cuestas","doi":"10.1016/j.ram.2024.12.010","DOIUrl":"https://doi.org/10.1016/j.ram.2024.12.010","url":null,"abstract":"<p><p>Protothecosis is an infectious disease caused by microalgae of the genus Prototheca. Prototheca can be found in soil and water and transiently colonize animals. Cutaneous protothecosis can involve not only the skin but also the underlying subcutaneous tissue and lymph nodes. This can lead to clinical signs and a microscopic tissue image that closely resembles a fungal infection. Treatment typically involves antifungal medications. We report the first case of fatal disseminated protothecosis caused by Prototheca wickerhamii in the city of Rosario, Argentina in a 5-year-old female poodle dog. The dog exhibited ocular signs of uveitis and lymphadenitis. To reach a clinical and etiological diagnosis, imaging studies, routine laboratory tests and serological tests were performed. A mycological analysis was conducted on the material obtained by puncturing three lymph nodes. Additionally, morphological, metabolic, and molecular analyses were performed. Antifungal susceptibility testing was also conducted using broth microdilution and diffusion methods. Phenotypic, metabolic, and sequencing techniques identified the isolated organism as P. wickerhamii. This isolate displayed susceptibility to amphotericin, variable susceptibility to itraconazole and voriconazole, and resistance to fluconazole and caspofungin. The frequent presence of pets in our homes highlights the need for a more comprehensive diagnostic approach. This is important because, from a public health perspective, dogs could serve as indicators of algal presence in the environments they frequently share with humans.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María Cecilia Mestre, Mariana E Langenheim, María Elena Severino, Sonia Fontenla
{"title":"Do psychrotolerant Patagonian soil yeasts produce 3-indole acetic acid?","authors":"María Cecilia Mestre, Mariana E Langenheim, María Elena Severino, Sonia Fontenla","doi":"10.1016/j.ram.2024.12.004","DOIUrl":"https://doi.org/10.1016/j.ram.2024.12.004","url":null,"abstract":"<p><p>Plant growth promoting microorganisms play a substantial role in current plant production practices. Yeasts have shown a promising role in enhancing plant production. We studied the production of indole 3-acetic acid (IAA)-like compounds in three psychrotolerant Patagonian native yeast strains. The yeast Tausonia pullulans CRUB 1772, Candida saitoana CRUB 1770 and Saccharomyces eubayanus CRUB 2014 were evaluated using the Salkowski's colorimetric technique and high-performance liquid chromatography (HPLC). T. pullulans CRUB 1772 was the highest producer of IAA-like compounds using the colorimetric technique. Nevertheless, none of the tested yeasts reached the production level of the bacterial strain A. argentinense Az39 used as positive control. The HPLC analysis revealed that only C. saitoana CRUB 1770 and S. eubayanus CRUB 2014 were able to truly produce IAA. Other peaks were also observed, which might correspond to intermediate compounds within the IAA biosynthetic pathway.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}