Eduardo Montalvo , Florencia Veiga , Hernán Rodríguez , German Traglia , Carlos Vay , Marisa Almuzara
{"title":"Identificación y sensibilidad antibiótica de aislamientos de Aeromonas spp. en un Hospital Universitario de la ciudad de Buenos Aires","authors":"Eduardo Montalvo , Florencia Veiga , Hernán Rodríguez , German Traglia , Carlos Vay , Marisa Almuzara","doi":"10.1016/j.ram.2024.11.001","DOIUrl":null,"url":null,"abstract":"<div><div><em>Aeromonas</em> spp. are opportunistic pathogens that cause both intra- and extraintestinal infections. The objective of this work was the phenotypic and genotypic characterization of a collection of <em>Aeromonas</em> strains, in addition to determining their sensitivity to different antimicrobials. Thirty seven isolates were analyzed. 54% were of intra-abdominal origin, 22% from skin and soft tissues, 19% from the bloodstream, among other less frequent sites. By amplification and sequencing of the <em>gyrB</em> gene, which was considered the reference method, the following were identified: 37,8% as species of the <em>Aeromonas hydrophila</em> complex, 32,4% as species of the <em>Aeromonas veronii</em> complex, and 29,7% as species of the complex <em>Aeromonas caviae</em>. Identification by traditional biochemical tests presented a better correlation with molecular identification than mass spectrometry (MALDI TOF MS). Regarding antibiotic sensitivity, cefotaxime, ceftazidime, cefepime, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, ciprofloxacin, amikacin, gentamicin and nitrofurantoin showed activity on more than 80.0% of the isolates tested. The sensitivity and specificity of the phenotypic methods to determine the presence of carbapenemases in relation to the detection of the cphAgene, the reference method, was 60,9% and 100%, respectively, for the colorimetric assay (Blue Carba), and of 91,3% and 50,0% respectively, for the modified Hodge test. The overall resistance to colistin was 32,4%. The automated method showed a very higher error (VME) of 16,2%, while the rapid colorimetric screening method (CRTc) showed an excellent correlation (VME 0%) with the reference method, broth microdilution.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 105-113"},"PeriodicalIF":1.8000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Argentina de microbiologia","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0325754124001330","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Aeromonas spp. are opportunistic pathogens that cause both intra- and extraintestinal infections. The objective of this work was the phenotypic and genotypic characterization of a collection of Aeromonas strains, in addition to determining their sensitivity to different antimicrobials. Thirty seven isolates were analyzed. 54% were of intra-abdominal origin, 22% from skin and soft tissues, 19% from the bloodstream, among other less frequent sites. By amplification and sequencing of the gyrB gene, which was considered the reference method, the following were identified: 37,8% as species of the Aeromonas hydrophila complex, 32,4% as species of the Aeromonas veronii complex, and 29,7% as species of the complex Aeromonas caviae. Identification by traditional biochemical tests presented a better correlation with molecular identification than mass spectrometry (MALDI TOF MS). Regarding antibiotic sensitivity, cefotaxime, ceftazidime, cefepime, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, ciprofloxacin, amikacin, gentamicin and nitrofurantoin showed activity on more than 80.0% of the isolates tested. The sensitivity and specificity of the phenotypic methods to determine the presence of carbapenemases in relation to the detection of the cphAgene, the reference method, was 60,9% and 100%, respectively, for the colorimetric assay (Blue Carba), and of 91,3% and 50,0% respectively, for the modified Hodge test. The overall resistance to colistin was 32,4%. The automated method showed a very higher error (VME) of 16,2%, while the rapid colorimetric screening method (CRTc) showed an excellent correlation (VME 0%) with the reference method, broth microdilution.
期刊介绍:
La Revista Argentina de Microbiología es una publicación trimestral editada por la Asociación Argentina de Microbiología y destinada a la difusión de trabajos científicos en las distintas áreas de la Microbiología. La Asociación Argentina de Microbiología se reserva los derechos de propiedad y reproducción del material aceptado y publicado.