Impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall structure and its influence on aflatoxin B₁ binding.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-20 DOI:10.1016/j.ram.2024.07.004

Carina Pereyra, María Del Pilar Monge, Silvestre Bongiovanni, Andrea Cristofolini, Sergio Campos, Lilia Cavaglieri

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Abstract

The aim of this study was to determine the impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall (CW) structure and its influence on aflatoxin B₁ binding. The yeast was inoculated into two types of culture media: yeast extract-peptone-dextrose (YPD) broth and dried distiller's grains with solubles (DDG). The CW was extracted from the biomass produced in these media. AFB₁ (150ng/ml) adsorption tests using the biomass (1×10⁷cells/ml) and the CW (0.001g) were performed at pH 2 and pH 8. Transmission electron microscopy (TEM) evaluated the CW thickness, and infrared spectroscopy (IR) determined the CW composition. Biomass production in YPD was higher than that in DDG. Cell diameter (μm) and CW thickness (μm) increased in the DDG medium. The CW percentage obtained in DDG was higher than that in YPD. The absorbance of carbohydrates by IR was higher in YPD. pH influenced AFB₁ adsorption, which was lower at pH 8. The proportion of β-glucan and chitin present in CW was higher in the YPD medium. The IR method allowed to study the CW carbohydrate variation under the influence of these carbon sources. In conclusion, the culture media composition influenced the β-glucan and chitin composition and consequently, AFB₁ adsorption.

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Kluyveromyces marxianus VM004 培养条件对细胞壁结构的影响及其对黄曲霉毒素 B1 结合力的影响。

本研究旨在确定马氏酵母菌 VM004 培养条件对细胞壁（CW）结构的影响及其对黄曲霉毒素 B1 结合力的影响。将酵母接种到两种培养基中：酵母提取物-蛋白胨-葡萄糖（YPD）肉汤和带溶质的干蒸馏谷物（DDG）。从这些培养基中产生的生物量中提取 CW。透射电子显微镜（TEM）评估了 CW 的厚度，红外光谱（IR）确定了 CW 的成分。YPD 中的生物质产量高于 DDG。在 DDG 培养基中，细胞直径（μm）和 CW 厚度（μm）均有所增加。在 DDG 中获得的 CW 百分比高于在 YPD 中获得的 CW 百分比。在 YPD 培养基中，碳水化合物的红外吸光度较高。 pH 值对 AFB1 的吸附有影响，pH 值为 8 时吸附较低。在 YPD 培养基中，CW 中的β-葡聚糖和甲壳素比例较高。红外方法有助于研究在这些碳源影响下 CW 碳水化合物的变化。总之，培养基组成影响了β-葡聚糖和甲壳素的组成，进而影响了对AFB1的吸附。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.