Reproductive biomedicine online最新文献

{"title":"Oocyte development: it's all about quality","authors":"Richard A. Anderson ,&nbsp;Adele L. Marston ,&nbsp;Evelyn E. Telfer","doi":"10.1016/j.rbmo.2025.104804","DOIUrl":"10.1016/j.rbmo.2025.104804","url":null,"abstract":"<div><div>Mammalian fertility depends on the production of an oocyte capable of fertilization and supporting early embryo development. This requires both cytoplasmic and nuclear, i.e. chromosomal, competence, processes that were initiated decades prior to ovulation. Current demographic changes with delayed motherhood are increasingly in conflict with these biological processes. This brief review highlights the key stages in oocyte development, as well as recent findings that continue to inform on how the oocyte is able to maintain function over such a prolonged period. These include minimizing oocyte damage caused by the production of reactive oxygen species, the importance of intercellular communication with the surrounding somatic cells, and the molecular mechanisms that underpin the fidelity of chromosome cohesion and then separation at the resumption of meiosis. Some of these are already approaching clinical testing and interventions, with new approaches in the coming years potentially being able to ‘put back the clock’ to improve oocyte quality.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104804"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaping the future of the IVF laboratory: standardization for more predictable outcomes","authors":"Mina Alikani ,&nbsp;Alison Campbell","doi":"10.1016/j.rbmo.2025.104854","DOIUrl":"10.1016/j.rbmo.2025.104854","url":null,"abstract":"<div><div>IVF is the foundational technology in the broader field of assisted reproductive technology (ART). The IVF laboratory is at the heart of ART, both as a major source of innovation and advancement and as a key to the success of the treatments. However, substantial variability in laboratory practices persists, despite longstanding efforts to establish uniform standards for improved reliability, reproducibility, and comparability. This commentary highlights the core factors contributing to disparity in outcomes and illustrates how emerging technologies, particularly artificial intelligence and robotics, offer new opportunities to propel standardization forwards. By integrating data-driven benchmarks and automated processes, including in quality management, the future IVF laboratory can achieve better and more predictable outcomes and advance the global standard of care.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104854"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolution of preimplantation genetic testing: where is the limit?","authors":"Antonio Capalbo ,&nbsp;Dagan Wells","doi":"10.1016/j.rbmo.2025.104845","DOIUrl":"10.1016/j.rbmo.2025.104845","url":null,"abstract":"<div><div>Preimplantation genetic testing (PGT) has revolutionized reproductive medicine over the past 30 years, providing a reliable method for reducing the risk of transmitting severe inherited conditions and offering the possibility of improved IVF outcomes. Today, PGT is widely accepted and integrated into fertility care in many countries around the world. Its history, however, has not been without controversy, with debate around its application to the diagnosis of late-onset disorders, conditions with incomplete penetrance and its use for embryo selection based upon human leukocyte antigen status. Nonetheless, PGT has progressively broadened its scope, and the number of embryos undergoing genetic testing continues to grow each year. Preimplantation genetic testing is most often used for the detection of chromosomal abnormalities, assisting in the identification of embryos affected by lethal aneuploidy. This application has generated the greatest debate of all, owing, in part, to difficulties delivering effective embryo testing using earlier methods. In recent years, advances in technology and rigorous validation studies have helped to improve accuracy, although variability among methods underscores the need for greater standardization and transparency. Emerging technologies, such as whole genome sequencing (WGS) and genome editing, hold promise for further advancements but introduce complex ethical, privacy and consent challenges that demand careful consideration, public engagement and thorough clinical research before implementation. Given its current trajectory, it seems likely that the use of PGT will continue to grow, offering reduced reproductive risks and the possibility of enhanced fertility treatment outcomes for ever greater numbers of patients, ultimately becoming an accepted cornerstone of reproductive care.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104845"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Twenty-five years of research in endometriosis","authors":"Juan A. Garcia-Velasco ,&nbsp;Paola Viganò ,&nbsp;Edgardo Somigliana","doi":"10.1016/j.rbmo.2025.104830","DOIUrl":"10.1016/j.rbmo.2025.104830","url":null,"abstract":"<div><div>Research rarely progresses in a straight line. It is more like an unpredictable front, marked by bursts of brilliance that fade after a few years, sudden breakthroughs, periods of stagnation, rediscoveries of forgotten ideas and occasional setbacks. Endometriosis research is no exception to these dynamics. In this opinion piece, three Generation X researchers who have been dedicated to endometriosis since the early days of their careers reflect on the field's progress over the past 25 years. Their verdict? Not disappointing. In fact, some pivotal strides have been made. The understanding of endometriosis has undergone a dramatic transformation. Hormonal therapies and assisted reproductive technology have emerged as first-line treatments, dethroning the once-central role of surgery. Non-invasive diagnosis of the disease has spread. This shift marks a notable evolution in how the disease is managed. However, high-throughput technologies have failed to deliver transformative insights, and the root causes of the disease remain as elusive as ever. Despite the setbacks, the progress made offers hope and direction.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104830"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to: Evaluating time-lapse imaging systems for embryo incubation and selection in ART: a critical review of a randomized controlled trial.","authors":"Priya Bhide, David Yl Chan, M Carmen Pardo, Teresa Perez, Khalid S Khan, Shakila Thangaratinam","doi":"10.1016/j.rbmo.2025.104938","DOIUrl":"https://doi.org/10.1016/j.rbmo.2025.104938","url":null,"abstract":"<p><p>The concerns raised by the authors of this commentary are largely addressed in the paper and the requested information is available in the supplementary materials and the published trial protocol referenced in the paper. We have further detailed some information to address their concerns. The planned effect size was based on the results reported by the largest randomized controlled trial at the start of our trial and supported by peer review and patient and clinician input. The trial was powered for the primary outcome of live birth, which we have reported. All reported secondary outcomes and subgroup analyses are only hypothesis generating.</p>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":" ","pages":"104938"},"PeriodicalIF":3.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating time-lapse imaging systems for embryo incubation and selection in ART: a critical review of a randomized controlled trial.","authors":"Alison Campbell, Dalit Ben-Yosef, Marcos Meseguer Escrivá, Thomas Freour","doi":"10.1016/j.rbmo.2025.104937","DOIUrl":"https://doi.org/10.1016/j.rbmo.2025.104937","url":null,"abstract":"<p><p>Time-lapse imaging has increasingly been introduced into IVF laboratories over the last decade, with some reported improvements over conventional practices reported in incubation conditions, workflow and embryo assessment and selection, although randomized controlled trials, with heterogeneous methodologies, have not demonstrated its superiority in clinical outcomes. A recent multicentre, three-parallel group, double-blind, randomized controlled trial assigned IVF or intracytoplasmic sperm injection patients in a 1:1:1 ratio to study the clinical effectiveness of time-lapse imaging systems for embryo incubation and selection. It concluded that the use of time-lapse imaging did not significantly increase the odds of live birth compared with standard care without time-lapse imaging. The current authors question the experimental design, methodology and reporting of results within that study and highlight the need for access to supplementary data to allow clinical professionals to analyse objectively and ultimately aim to determine whether this technology may improve the quality of care of their patients.</p>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":" ","pages":"104937"},"PeriodicalIF":3.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of trophectoderm biopsy for preimplantation genetic testing in the serum level of first trimester biomarkers","authors":"Pilar Prats ,&nbsp;María Gabriela Palacios-Verdú ,&nbsp;Alberto Rodríguez-Melcón ,&nbsp;Ignacio Rodríguez ,&nbsp;Bernat Serra ,&nbsp;Mónica Parriego ,&nbsp;Valeria Donno ,&nbsp;Nikolaos P. Polyzos","doi":"10.1016/j.rbmo.2024.104490","DOIUrl":"10.1016/j.rbmo.2024.104490","url":null,"abstract":"<div><h3>Research question</h3><div>Does trophectoderm biopsy for preimplantation genetic testing for aneuploidies (PGT-A) affect maternal serum first-trimester pregnancy biomarkers (pregnancy-associated plasma protein A [PAPP-A], free β-HCG and placental growth factor [PIGF])?</div></div><div><h3>Design</h3><div>Retrospective cohort study of all singleton pregnancies (<em>n</em> = 9794) after naturally conceived (<em>n</em> = 8005) IVF and fresh embryo transfers (<em>n</em> = 478), frozen embryo transfer of non PGT-A (FET) (<em>n</em> = 963) or PGT-A tested embryos (FET + PGT-A) (<em>n</em> = 348). Serum levels of free β-HCG and PAPP-A were measured in all women with a viable pregnancy at 8–13.6 weeks of pregnancy; PIGF was measured in 3784 women. Biomarkers were converted to a multiple of the expected normal median (MOM) for a pregnancy of the same gestational day. The medians for the multiple of the median were calculated and compared.</div></div><div><h3>Results</h3><div>Free β-HCG did not differ according to mode of conception. The PAPP-A concentrations were significantly lower in IVF and fresh embryo transfers (–0.1 Log10 MOM raw PAPP-A) compared with FET + PGT-A (–0.04 Log 10 MOM raw PAPP-A, <em>P</em> = 0.009) and natural conceptions (–0.0187 Log 10 MOM raw PAPP-A) (<em>P</em> &lt; 0.001). The PIGF levels were significantly lower in the FET + PGTA group versus natural conception (<em>P</em> = 0.001). Difference in means adjusted by crown rump length was 4.6 pg/ml (95% CI 2.7 to 6.6) for natural conceptions, 3.5 pg/ml (95% CI 0.34 to 6.6) for IVF and 2.2 pg/ml (95% CI 0.06 to 4.4) for FET.</div></div><div><h3>Conclusion</h3><div>Trophectoderm biopsy for PGT-A has a significant effect on first-trimester maternal serum PAPP-A and PIGF. This needs to be further validated, as it may mislead the estimation of the first-trimester risk of aneuploidies and pre-eclampsia.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104490"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-intensity-pulsed-ultrasound-treated menstrual-blood-derived mesenchymal stem cells repair endometrial injury by PI3K/AKT pathway inhibition","authors":"Shuangying Huang ,&nbsp;Chunxia Cheng ,&nbsp;Zhibiao Wang ,&nbsp;Ruizhen Li ,&nbsp;Waixing Li ,&nbsp;Kenan Yu ,&nbsp;Qiuling Shi ,&nbsp;Min Xue","doi":"10.1016/j.rbmo.2024.104486","DOIUrl":"10.1016/j.rbmo.2024.104486","url":null,"abstract":"<div><h3>Research question</h3><div>What is the effect and underlying mechanism of low-intensity-pulsed-ultrasound (LIPUS)-treated menstrual-blood-derived mesenchymal stem cells (MenSC) on intrauterine adhesions (IUA)?</div></div><div><h3>Design</h3><div>First, MenSC were identified and exposed to LIPUS. The proliferation, migration, invasion, cytokine secretion, and ability to differentiate into human endometrial epithelial cells (HEEC) of LIPUS-treated MenSC were characterized. <em>In vitro</em>, human endometrial stromal cells (HESC) were treated with 10 ng/ml transforming growth factor-β1 (TGF-β1) to simulate IUA, and then co-cultured indirectly with LIPUS-treated MenSC. <em>In vivo</em>, IUA rat models were constructed and LIPUS-treated MenSC were transplanted into the uterus. The morphology, structure, and levels of fibrosis and repair-related factors of the uterus were detected. In the mechanism study, insulin-like growth factor-1 (IGF-1) was applied to verify whether the PI3K/AKT pathway participated in the repair of endometrial injury by LIPUS-treated MenSC.</div></div><div><h3>Results</h3><div><em>In vitro</em>, LIPUS treatment showed beneficial effects on MenSC by promoting cell proliferation and migration; inhibiting apoptosis; and enhancing the expression of epidermal growth factor, hepatocyte growth factor and vascular endothelial growth factor. It also facilitated the differentiation of MenSC into HEEC while reducing the level of fibrosis in TGF-β1-treated HESC by decreasing the concentrations of p-PI3K and p-AKT. However, these effects were reversed with the use of IGF-1. <em>In vivo</em>, transplantation of LIPUS-treated MenSC resulted in increased uterine length, width and weight. The transplanted cells also improved completeness of the endometrial structure, reduced inflammatory infiltration, increased endometrial thickness and gland abundance, and decreased endometrial fibrosis. Additionally, increased concentations of endometrial-repair-related proteins and receptivity-related markers were observed after transplantation of LIPUS-treated MenSC.</div></div><div><h3>Conclusion</h3><div>LIPUS-treated MenSC repaired endometrial injury by inhibiting the PI3K/AKT pathway.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104486"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oocyte retrieval of few follicles does not require analgesia: a large-scale multicentre pain analysis","authors":"Isotta M. Magaton ,&nbsp;Martina Nordin ,&nbsp;Ikbale Siercks ,&nbsp;Roxana M. Popovici ,&nbsp;Eva Boogen ,&nbsp;Stefan Eisenhardt ,&nbsp;Cosima Huober-Zeeb ,&nbsp;Jan-Simon Lanowski ,&nbsp;Marie Roumet ,&nbsp;Michael von Wolff","doi":"10.1016/j.rbmo.2024.104696","DOIUrl":"10.1016/j.rbmo.2024.104696","url":null,"abstract":"<div><h3>Research question</h3><div>What is the pain intensity of oocyte retrieval (OCR) without anaesthesia and analgesia in women with a low or medium number of follicles?</div></div><div><h3>Design</h3><div>Multicentre observational study analysing IVF cycles without anaesthesia and analgesia in seven IVF centres in Germany and Switzerland between January 2022 and March 2023. In total, 2290 cycles of natural cycle IVF or minimal stimulation IVF from 1039 patients were recorded. Pain score was assessed using a VAS graded 0 to 10. A descriptive analysis was conducted, followed by a statistical evaluation using a linear mixed model to analyse the effect on the pain score of the number of oocytes retrieved and the number of repeated IVF cycles.</div></div><div><h3>Results</h3><div>The number of oocytes retrieved varied from 0 to 16 (IQR 1–2). Pain score varied from 0 to 10, with a median pain score of 3.0 (IQR 2–4), defined as ‘mild’ pain. Compared with a reference value of aspirations with one oocyte, the pain score increased slightly by 0.5 points if no oocyte was retrieved, by 0.42 points if two to five oocytes were retrieved and by 1.14 points if six or more oocytes were retrieved, all with little clinical relevance. Pain decreased slightly with repeated aspirations (–0.05 points per cycle) (<em>P</em> = 0.028). The complication rate requiring hospitalization per aspiration was less than 0.1%.</div></div><div><h3>Conclusion</h3><div>Pain intensity during OCR of one or few follicles is mild. Therefore, OCR can be offered without anaesthesia and analgesia to reduce aspiration time and cost.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104696"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use and understanding of AI in the ART laboratory: an international survey","authors":"Giles Anthony Palmer ,&nbsp;Omar Paredes ,&nbsp;Andrew Drakeley ,&nbsp;Alejandro Chavez-Badiola ,&nbsp;Thomas E. Woolley ,&nbsp;Katerina Kaouri ,&nbsp;Jacques Cohen","doi":"10.1016/j.rbmo.2024.104435","DOIUrl":"10.1016/j.rbmo.2024.104435","url":null,"abstract":"<div><h3>Research question</h3><div>What is the awareness, adoption and comprehension of artificial intelligence (AI) among assisted reproductive technology (ART) laboratory professionals?</div></div><div><h3>Design</h3><div>A cross-sectional survey consisting of 32 questions was conducted among clinical embryologists worldwide using an online questionnaire between 17 July and 31 August 2023. The survey assessed familiarity with AI technology; current knowledge within laboratories; understanding of AI principles and limitations; and views on ethical concerns, job impacts and scientist–patient relationships.</div></div><div><h3>Results</h3><div>In total, there were 702 survey respondents. The results revealed a high degree of awareness of AI concepts. The participants recognized the potential benefits of AI in embryology, but acknowledged known limitations. While open to the adoption of AI, they expressed reservations surrounding ethics, effects on jobs, and maintaining positive patient relationships. The study uncovered differences in embryologists' opinions based on their years of experience. Most embryologists, independent of age, were positive regarding AI, but workplace concerns diminished with age.</div></div><div><h3>Conclusions</h3><div>ART professionals are broadly receptive to AI, but ethical and practical uncertainties were raised. Further engagement between developers and end-users can align AI innovation with the values and needs of human practitioners.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104435"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143410391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}