Reproductive biomedicine online最新文献

{"title":"Corrigendum to 'Biological characteristics related to treatment effects of the levonorgestrel-releasing intrauterine system on adenomyosis-associated dysmenorrhoea' Reproductive BioMedicine Online, 2024, 49;6:104393.","authors":"Qiutong Li, Dai Yi, Xiaoyan Li, Yushi Wu, Zhiyue Gu, Chenyu Zhang, Hailan Yan, Shiqing Lyu, Biyun Zhang, Jinghua Shi, Jinhua Leng","doi":"10.1016/j.rbmo.2025.104829","DOIUrl":"https://doi.org/10.1016/j.rbmo.2025.104829","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":" ","pages":"104829"},"PeriodicalIF":3.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ignored structure in female fertility: cilia in the fallopian tubes","authors":"Liuqing He ,&nbsp;Haofei Xu ,&nbsp;Min Liu ,&nbsp;Ying Tan ,&nbsp;Shiyu Huang ,&nbsp;Xiaoxiao Yin ,&nbsp;Xinyu Luo ,&nbsp;Hui Yee Chung ,&nbsp;Ming Gao ,&nbsp;Yujie Li ,&nbsp;Weijun Ding ,&nbsp;Hang Zhou ,&nbsp;Yefang Huang","doi":"10.1016/j.rbmo.2024.104346","DOIUrl":"10.1016/j.rbmo.2024.104346","url":null,"abstract":"<div><div>Cilia in the fallopian tubes (CFT) play an important role in female infertility, but have not been explored comprehensively. This review reveals the detection techniques for CFT function and morphology, and the related analysis of female infertility and other gynaecological disorders. CFT differentiate from progenitor cells, and develop into primary cilia and motile cilia. Primary cilia coordinate multiple signalling pathways, and motile cilia produce laminar flow through bidirectional intraflagellar transport, which drives the movement of oocytes and gametes. Several methods for quantitative detection and protein analysis have been used to explore the factors contributing to the decrease in ciliary beat frequency (CBF), and the cellular mechanism of ciliary cell death and shedding. In both primary and secondary ciliary disorders associated with reproductive diseases, abnormal alterations in ciliary quantity, ciliary structure, CBF and ciliary signalling pathways result in abnormal tubal laminar flow, and diminished oocyte retrieval and transport capabilities.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 2","pages":"Article 104346"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141701197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced endometrial glycolysis concomitant with increased lesional fibrosis in patients with adenomyosis who complained of heavy menstrual bleeding","authors":"Chenyu Mao ,&nbsp;Xishi Liu ,&nbsp;Sun-Wei Guo","doi":"10.1016/j.rbmo.2024.104406","DOIUrl":"10.1016/j.rbmo.2024.104406","url":null,"abstract":"<div><h3>Research question</h3><div>What role, if any, does the extent of lesional fibrosis play in impaired glycolysis leading to adenomyosis-associated heavy menstrual bleeding (ADM-HMB)?</div></div><div><h3>Design</h3><div>Forty-eight patients with ADM-HMB were recruited, among them 25 reported moderate to heavy bleeding (MHB), and the remaining 23, excessive bleeding (EXB). The full-thickness uterine tissue columns were processed for Masson trichrome staining and immunohistochemistry analyses. The expression levels of HIF-1α, GLUT1, HK2, PFKFB3 and PKM2 proteins that are critically involved in glycolysis in endometrial epithelial cells cultured on substrates of different stiffness, and the levels of glycolysis were quantitated. A mouse experiment with induced adenomyosis and simulated menstrual bleeding was conducted to assess the effect of adenomyosis on immunoexpression of proteins involved in glycolysis and inflammation as well as on endometrial repair and bleeding<em>.</em></div></div><div><h3>Results</h3><div>The endometrial staining of HIF-1α, GLUT1, HK2, PFKFB3 and PKM2 was significantly lower in the EXB group as compared with MHB patients, concomitant with higher extent of fibrosis. The expression of HIF-1α, GLUT1, HK2, PFKFB3 and PKM2 was significantly reduced when endometrial epithelial cells were cultured in stiff substrate, concomitant with reduced glycolysis. Mice with induced adenomyosis had reduced immunoexpression of Hif-1α, as well as those proteins each of which plays a vital, rate-limiting role in different steps of the glycolysis pathway, such as Glut1, Hk2, Pfkfb3 and Pkm2, and elevated fibrosis in endometrium, concomitant with disrupted endometrial repair and more bleeding.</div></div><div><h3>Conclusions</h3><div>Lesional fibrosis results in reduced endometrial glycolysis in eutopic endometrium and subsequent imbalance in pro-inflammatory and anti-inflammatory response, leading to ADM-HMB.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 2","pages":"Article 104406"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into the effects of endometriosis on IVF","authors":"Norbert Gleicher ,&nbsp;David H. Barad","doi":"10.1016/j.rbmo.2024.104482","DOIUrl":"10.1016/j.rbmo.2024.104482","url":null,"abstract":"<div><div>It is not uncommon that a published paper offers unintended insights, unnoticed by its authors. This was to a substantial degree the case with a recent publication addressing the effects of endometriosis on IVF. Using donor–recipient cycles as the study population to isolate recipient effects, the well-executed study demonstrated only mildly adverse outcome effects of endometriosis on IVF cycle outcomes, to a substantial degree laying to rest this still controversial issue. In the process, however, the study also raised some very interesting – but left undiscussed – insights into a host of other issues with considerable relevance to endometriosis and IVF practice in the USA and UK. These are the subject of this communication.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 2","pages":"Article 104482"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The assessment of oxidative stress in human semen: chaos and confusion in pursuit of diagnostic precision","authors":"Robert John Aitken ,&nbsp;Parviz Gharagozloo","doi":"10.1016/j.rbmo.2024.104488","DOIUrl":"10.1016/j.rbmo.2024.104488","url":null,"abstract":"<div><div>The importance of oxidative stress in the aetiology of male infertility has occasioned numerous clinical trials designed to assess the potential of antioxidants for treating this condition. These trials have not returned definitive results, probably because they have never selected participants on the basis of oxidative stress. Clearly, if a moderate to severe state of oxidative stress does not exist in semen, antioxidants can hardly be expected to improve fertility. To resolve this issue, robust, user-friendly point-of-care assays need to be developed that will enable clinicians to quickly diagnose and monitor oxidative stress in patients’ semen. Traditional assays of total antioxidant capacity do not fulfil this role, because they are time consuming, expensive and laboratory based. The introduction of an alternative electrochemical system (MiOXSYS®) was designed to address this problem. This assay records the static oxido-reductive potential of semen and then creates an index by dividing this measurement by the sperm concentration. The creation of such an index is flawed and undermines many of the data generated to date. This commentary explains the nature of this problem and emphasizes the continuing unmet need for effective diagnostic procedures capable of detecting seminal oxidative stress in the patient population.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 2","pages":"Article 104488"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning tool for predicting mature oocyte yield and trigger day from start of stimulation: towards personalized treatment","authors":"Akhil Garg ,&nbsp;Jose Bellver ,&nbsp;Ernesto Bosch ,&nbsp;José Alejandro Remohí ,&nbsp;Antonio Pellicer ,&nbsp;Marcos Meseguer","doi":"10.1016/j.rbmo.2024.104441","DOIUrl":"10.1016/j.rbmo.2024.104441","url":null,"abstract":"<div><div>Research question: Can machine learning tools predict the number of metaphase II (MII) oocytes and trigger day at the start of the ovarian stimulation cycle?</div><div>Design: A multicentre, retrospective study including 56,490 ovarian stimulation cycles (primary dataset) was carried out between 2020 and 2022 for analysis and feature selection. Of these, 13,090 were used to develop machine learning models for trigger day and the number of MII prediction, and another 5103 ovarian stimulation cycles (clinical validation dataset) from 2023 for clinical validation. Machine learning algorithms using deep learning were developed using optimal features from the primary dataset based on correlation.</div><div>Results: A tool with two novel progressive machine learning algorithms using deep learning was able to predict the trigger day and number of MII oocytes: mean absolute error 1.60 (95% CI 1.56 to 1.64) and 3.75 (95% CI 3.65 to 3.86), respectively. The R² value for the algorithm to predict the number of MII in the interquartile (Q3–Q1/P75–P25) range was 0.88; the entire dataset was 0.70 after removing the outliers at the planning phase of the stimulation cycle, which shows high accuracy. The interquartile root mean square error was 1.10 and 0.66 for the trigger day and the number of oocytes algorithm, respectively.</div><div>Conclusion: The tool using deep learning algorithms has high prediction power for trigger day and number of MII outcomes, and can be retrieved from patients at the start of the ovarian stimulation cycle; however, inclusion of more data and validation from different clinics are needed.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 2","pages":"Article 104441"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tailor-made embryo transfer considering embryonic developmental speed to overcome the dilemma of personalized embryo transfer","authors":"Yasuhiro Ohara ,&nbsp;Hidehiko Matsubayashi ,&nbsp;Shimpei Mizuta ,&nbsp;Masakazu Doshida ,&nbsp;Takumi Takeuchi ,&nbsp;Tomomoto Ishikawa ,&nbsp;Mika Handa ,&nbsp;Tatsuya Miyake ,&nbsp;Tsuyoshi Takiuchi ,&nbsp;Tadashi Kimura","doi":"10.1016/j.rbmo.2024.104405","DOIUrl":"10.1016/j.rbmo.2024.104405","url":null,"abstract":"<div><h3>Research Question</h3><div>Does tailor-made embryo transfer (TmET), timed with respect to embryonic developmental speed, affect pregnancy outcomes in patients with recurrent implantation failure?</div></div><div><h3>Design</h3><div>Among 741 patients identified as receptive through endometrial receptivity testing, the clinical pregnancy rates and live birth rates were retrospectively compared between those who underwent standard personalized embryo transfer and those who underwent TmET in hormone replacement therapy cycles. Personalized embryo transfer was performed according to endometrial receptivity test results (standard personalized embryo transfer group) or considering embryonic developmental speed (TmET group). For TmET, the expansion grade of warmed blastocysts was estimated based on each patient's previous embryonic developmental pattern. The embryo transfer days were set so that estimated blastocyst grades 3, 4, 5 and 6 were transferred on days P+5, P+5.5, P+6.0 and P+6.5, respectively.</div></div><div><h3>Results</h3><div>In a propensity score matching analysis, the clinical pregnancy rate was significantly higher in the TmET group than the standard personalized embryo transfer group (<em>P</em> = 0.014), whereas the live birth rates were similar between the two groups (<em>P</em> = 0.65). In a subgroup analysis with euploid embryo transfers, the clinical pregnancy rate was significantly higher in the TmET group than the standard personalized embryo transfer group, although there was no difference in live birth rate between the two groups (<em>P</em> = 0.045 and <em>P</em> = 0.057, respectively).</div></div><div><h3>Conclusions</h3><div>For patients experiencing recurrent implantation failure and identified as receptive through endometrial receptivity testing, subsequent TmET strategies may further enhance pregnancy outcomes.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 2","pages":"Article 104405"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Family creation by combined use of ART and surrogacy in a transgender couple: a unique case report","authors":"Kamal K. Ahuja,&nbsp;Giuseppina Lamanna,&nbsp;Nick Macklon","doi":"10.1016/j.rbmo.2024.104487","DOIUrl":"10.1016/j.rbmo.2024.104487","url":null,"abstract":"<div><div>In 2014 a 36-year-old healthy female-to-male transgender patient attended the London Women's Clinic to consider oocyte and embryo freezing before sex reassignment surgery. The patient began IVF treatment in 2015; from two cycles, nine metaphase II oocytes and five blastocysts were frozen. Three years later the patient returned with his partner, a 39-year-old healthy transgender male-to-female individual, ready to start a family with surrogacy treatment. The surrogate delivered a healthy baby girl born at term in 2021 via Caesarean section, with a second successfully delivered in 2022. To our knowledge this is the first case report of successful family creation in which both partners are transgender.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 2","pages":"Article 104487"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of pipette- and laser-induced blastocyst collapse before vitrification on their re-expansion and clinical outcome after warming","authors":"Iris Martínez-Rodero ,&nbsp;Borut Kovačič ,&nbsp;Omar Shebl ,&nbsp;Sabine Enengl ,&nbsp;Julia Lastinger ,&nbsp;Thomas Ebner","doi":"10.1016/j.rbmo.2024.104476","DOIUrl":"10.1016/j.rbmo.2024.104476","url":null,"abstract":"<div><h3>Research question</h3><div>What are the effects of pipette- versus laser-assisted artificial blastocyst collapse (ABC) on the morphokinetics of warmed blastocyst re-expansion, and what is the potential effect on treatment outcomes?</div></div><div><h3>Design</h3><div>Surplus blastocysts were extracted from 203 patients. These were divided into three groups: study group A, artificial collapsed by the aspiration of blastocoel fluid with a pipette; study group B, trophectoderm opened with a laser pulse; control group, no manipulation before vitrification was performed. During the 5-year study period, 257 associated single-warm blastocyst transfers were scheduled. The start and duration of the re-expansion process before transfer were annotated. Pregnancy and live birth data were also collected for the transfers.</div></div><div><h3>Results</h3><div>The overall blastocyst survival rate was 96.9%, with no effect observed as a result of the two ABC methods. The re-expansion of blastocysts in study group B was initiated significantly sooner after warming (0.50 ± 0.37 h) than in group A (0.79 ± 0.56 h) or the control group (1.22 ± 1.00 h). The duration of the re-expansion process was significantly reduced in study groups A (<em>P</em> = 0.021) and B (<em>P</em> = 0.004) compared with the control group. The embryos of participants who achieved a live birth had a significantly (<em>P</em> &lt; 0.001) faster start of re-expansion (0.60 ± 0.42 h) than the embryos in those who did not produce an ongoing pregnancy (1.05 ± 0.92 h).</div></div><div><h3>Conclusions</h3><div>Laser-treated blastocysts exhibited substantially shorter re-expansion times. Because faster re-expansion of the blastocyst is associated with positive treatment outcomes, the laser technique should be prioritized over the pipetting technique if ABC is considered.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 2","pages":"Article 104476"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of long versus short co-incubation of gametes on post-insemination outcomes and embryo morphokinetics","authors":"Aşina Bayram ,&nbsp;Neelke De Munck ,&nbsp;Ibrahim Elkhatib ,&nbsp;Erkan Kalafat ,&nbsp;Andrea Abdala ,&nbsp;Virginia Ferracuti ,&nbsp;Laura Melado ,&nbsp;Barbara Lawrenz ,&nbsp;Human Fatemi ,&nbsp;Daniela Nogueira","doi":"10.1016/j.rbmo.2024.104480","DOIUrl":"10.1016/j.rbmo.2024.104480","url":null,"abstract":"<div><h3>Research question</h3><div>Does a short co-incubation of gametes in conventional IVF affect post-insemination outcomes and embryo morphokinetics?</div></div><div><h3>Design</h3><div>Sibling oocyte randomized pilot study conducted between December 2020 and March 2023. Eligible couples (<em>n</em> = 55) were women aged 18–43 years with BMI 35 km/m² or lower and male normal semen parameters. Cumulus oocyte complexes (COC) (six to 12) were randomized in a 1:1:2 proportion in long (16–18 h) or short (2 h) co-incubation IVF exposure and ICSI, respectively. All oocytes inseminated via IVF were monitored by time-lapse. Blastocysts graded BL3CC or higher underwent trophectoderm biopsy on days 5, 6 or 7, and were analysed by next-generation sequencing.</div></div><div><h3>Results</h3><div>A total of 954 COC were distributed to long co-incubation (<em>n</em> = 235 [24.6%]), short co-incubation (<em>n</em> = 235 [24.6%]) and ICSI (<em>n</em> = 484 [50.7%]). In total, 202 were MII after long co-incubation, 209 after short co-incubation and 394 at ICSI. Regression analyses showed that short co-incubation groups (OR 0.72, 95% CI 0.46 to 1.11, <em>P</em> = 0.139) and ICSI (OR 1.43, 95% CI 0.95 to 2.15, <em>P</em> = 0.090) did not significantly affect normal fertilization rates (2PN) compared with long co-incubation. Usable blastocysts per MII, per 2PN, and euploid blastocysts per MII, per 2PN and per tested blastocysts were similar among the groups. The rate of embryo arrest (hazard ratio [HR] 0.79, 95% CI 0.57 to 1.11, <em>P</em> = 0.183) and timings to reach morphokinetic milestones among embryos reaching blastulation were similar between IVF groups (<em>P</em> &gt; 0.05 for all).</div></div><div><h3>Conclusion</h3><div>A short 2-h co-incubation of gametes in IVF yields comparable fertilization, blastocyst and euploidy rates without adverse embryo morphokinetic events, compared with standard overnight co-incubation.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 2","pages":"Article 104480"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}