Reproductive biomedicine online最新文献

{"title":"Influence of trophectoderm biopsy for preimplantation genetic testing in the serum level of first trimester biomarkers","authors":"Pilar Prats ,&nbsp;María Gabriela Palacios-Verdú ,&nbsp;Alberto Rodríguez-Melcón ,&nbsp;Ignacio Rodríguez ,&nbsp;Bernat Serra ,&nbsp;Mónica Parriego ,&nbsp;Valeria Donno ,&nbsp;Nikolaos P. Polyzos","doi":"10.1016/j.rbmo.2024.104490","DOIUrl":"10.1016/j.rbmo.2024.104490","url":null,"abstract":"<div><h3>Research question</h3><div>Does trophectoderm biopsy for preimplantation genetic testing for aneuploidies (PGT-A) affect maternal serum first-trimester pregnancy biomarkers (pregnancy-associated plasma protein A [PAPP-A], free β-HCG and placental growth factor [PIGF])?</div></div><div><h3>Design</h3><div>Retrospective cohort study of all singleton pregnancies (<em>n</em> = 9794) after naturally conceived (<em>n</em> = 8005) IVF and fresh embryo transfers (<em>n</em> = 478), frozen embryo transfer of non PGT-A (FET) (<em>n</em> = 963) or PGT-A tested embryos (FET + PGT-A) (<em>n</em> = 348). Serum levels of free β-HCG and PAPP-A were measured in all women with a viable pregnancy at 8–13.6 weeks of pregnancy; PIGF was measured in 3784 women. Biomarkers were converted to a multiple of the expected normal median (MOM) for a pregnancy of the same gestational day. The medians for the multiple of the median were calculated and compared.</div></div><div><h3>Results</h3><div>Free β-HCG did not differ according to mode of conception. The PAPP-A concentrations were significantly lower in IVF and fresh embryo transfers (–0.1 Log10 MOM raw PAPP-A) compared with FET + PGT-A (–0.04 Log 10 MOM raw PAPP-A, <em>P</em> = 0.009) and natural conceptions (–0.0187 Log 10 MOM raw PAPP-A) (<em>P</em> &lt; 0.001). The PIGF levels were significantly lower in the FET + PGTA group versus natural conception (<em>P</em> = 0.001). Difference in means adjusted by crown rump length was 4.6 pg/ml (95% CI 2.7 to 6.6) for natural conceptions, 3.5 pg/ml (95% CI 0.34 to 6.6) for IVF and 2.2 pg/ml (95% CI 0.06 to 4.4) for FET.</div></div><div><h3>Conclusion</h3><div>Trophectoderm biopsy for PGT-A has a significant effect on first-trimester maternal serum PAPP-A and PIGF. This needs to be further validated, as it may mislead the estimation of the first-trimester risk of aneuploidies and pre-eclampsia.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104490"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use and understanding of AI in the ART laboratory: an international survey","authors":"Giles Anthony Palmer ,&nbsp;Omar Paredes ,&nbsp;Andrew Drakeley ,&nbsp;Alejandro Chavez-Badiola ,&nbsp;Thomas E. Woolley ,&nbsp;Katerina Kaouri ,&nbsp;Jacques Cohen","doi":"10.1016/j.rbmo.2024.104435","DOIUrl":"10.1016/j.rbmo.2024.104435","url":null,"abstract":"<div><h3>Research question</h3><div>What is the awareness, adoption and comprehension of artificial intelligence (AI) among assisted reproductive technology (ART) laboratory professionals?</div></div><div><h3>Design</h3><div>A cross-sectional survey consisting of 32 questions was conducted among clinical embryologists worldwide using an online questionnaire between 17 July and 31 August 2023. The survey assessed familiarity with AI technology; current knowledge within laboratories; understanding of AI principles and limitations; and views on ethical concerns, job impacts and scientist–patient relationships.</div></div><div><h3>Results</h3><div>In total, there were 702 survey respondents. The results revealed a high degree of awareness of AI concepts. The participants recognized the potential benefits of AI in embryology, but acknowledged known limitations. While open to the adoption of AI, they expressed reservations surrounding ethics, effects on jobs, and maintaining positive patient relationships. The study uncovered differences in embryologists' opinions based on their years of experience. Most embryologists, independent of age, were positive regarding AI, but workplace concerns diminished with age.</div></div><div><h3>Conclusions</h3><div>ART professionals are broadly receptive to AI, but ethical and practical uncertainties were raised. Further engagement between developers and end-users can align AI innovation with the values and needs of human practitioners.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104435"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143410391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-intensity-pulsed-ultrasound-treated menstrual-blood-derived mesenchymal stem cells repair endometrial injury by PI3K/AKT pathway inhibition","authors":"Shuangying Huang ,&nbsp;Chunxia Cheng ,&nbsp;Zhibiao Wang ,&nbsp;Ruizhen Li ,&nbsp;Waixing Li ,&nbsp;Kenan Yu ,&nbsp;Qiuling Shi ,&nbsp;Min Xue","doi":"10.1016/j.rbmo.2024.104486","DOIUrl":"10.1016/j.rbmo.2024.104486","url":null,"abstract":"<div><h3>Research question</h3><div>What is the effect and underlying mechanism of low-intensity-pulsed-ultrasound (LIPUS)-treated menstrual-blood-derived mesenchymal stem cells (MenSC) on intrauterine adhesions (IUA)?</div></div><div><h3>Design</h3><div>First, MenSC were identified and exposed to LIPUS. The proliferation, migration, invasion, cytokine secretion, and ability to differentiate into human endometrial epithelial cells (HEEC) of LIPUS-treated MenSC were characterized. <em>In vitro</em>, human endometrial stromal cells (HESC) were treated with 10 ng/ml transforming growth factor-β1 (TGF-β1) to simulate IUA, and then co-cultured indirectly with LIPUS-treated MenSC. <em>In vivo</em>, IUA rat models were constructed and LIPUS-treated MenSC were transplanted into the uterus. The morphology, structure, and levels of fibrosis and repair-related factors of the uterus were detected. In the mechanism study, insulin-like growth factor-1 (IGF-1) was applied to verify whether the PI3K/AKT pathway participated in the repair of endometrial injury by LIPUS-treated MenSC.</div></div><div><h3>Results</h3><div><em>In vitro</em>, LIPUS treatment showed beneficial effects on MenSC by promoting cell proliferation and migration; inhibiting apoptosis; and enhancing the expression of epidermal growth factor, hepatocyte growth factor and vascular endothelial growth factor. It also facilitated the differentiation of MenSC into HEEC while reducing the level of fibrosis in TGF-β1-treated HESC by decreasing the concentrations of p-PI3K and p-AKT. However, these effects were reversed with the use of IGF-1. <em>In vivo</em>, transplantation of LIPUS-treated MenSC resulted in increased uterine length, width and weight. The transplanted cells also improved completeness of the endometrial structure, reduced inflammatory infiltration, increased endometrial thickness and gland abundance, and decreased endometrial fibrosis. Additionally, increased concentations of endometrial-repair-related proteins and receptivity-related markers were observed after transplantation of LIPUS-treated MenSC.</div></div><div><h3>Conclusion</h3><div>LIPUS-treated MenSC repaired endometrial injury by inhibiting the PI3K/AKT pathway.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104486"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oocyte retrieval of few follicles does not require analgesia: a large-scale multicentre pain analysis","authors":"Isotta M. Magaton ,&nbsp;Martina Nordin ,&nbsp;Ikbale Siercks ,&nbsp;Roxana M. Popovici ,&nbsp;Eva Boogen ,&nbsp;Stefan Eisenhardt ,&nbsp;Cosima Huober-Zeeb ,&nbsp;Jan-Simon Lanowski ,&nbsp;Marie Roumet ,&nbsp;Michael von Wolff","doi":"10.1016/j.rbmo.2024.104696","DOIUrl":"10.1016/j.rbmo.2024.104696","url":null,"abstract":"<div><h3>Research question</h3><div>What is the pain intensity of oocyte retrieval (OCR) without anaesthesia and analgesia in women with a low or medium number of follicles?</div></div><div><h3>Design</h3><div>Multicentre observational study analysing IVF cycles without anaesthesia and analgesia in seven IVF centres in Germany and Switzerland between January 2022 and March 2023. In total, 2290 cycles of natural cycle IVF or minimal stimulation IVF from 1039 patients were recorded. Pain score was assessed using a VAS graded 0 to 10. A descriptive analysis was conducted, followed by a statistical evaluation using a linear mixed model to analyse the effect on the pain score of the number of oocytes retrieved and the number of repeated IVF cycles.</div></div><div><h3>Results</h3><div>The number of oocytes retrieved varied from 0 to 16 (IQR 1–2). Pain score varied from 0 to 10, with a median pain score of 3.0 (IQR 2–4), defined as ‘mild’ pain. Compared with a reference value of aspirations with one oocyte, the pain score increased slightly by 0.5 points if no oocyte was retrieved, by 0.42 points if two to five oocytes were retrieved and by 1.14 points if six or more oocytes were retrieved, all with little clinical relevance. Pain decreased slightly with repeated aspirations (–0.05 points per cycle) (<em>P</em> = 0.028). The complication rate requiring hospitalization per aspiration was less than 0.1%.</div></div><div><h3>Conclusion</h3><div>Pain intensity during OCR of one or few follicles is mild. Therefore, OCR can be offered without anaesthesia and analgesia to reduce aspiration time and cost.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104696"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Editorial Board","authors":"","doi":"10.1016/S1472-6483(25)00100-2","DOIUrl":"10.1016/S1472-6483(25)00100-2","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104893"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143641838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of tubulin post-translational modifications and their enzymes during human oocyte meiosis","authors":"Paraskevi Karamtzioti ,&nbsp;Anna Ferrer-Vaquer ,&nbsp;Montserrat Barragan ,&nbsp;Isabelle Vernos ,&nbsp;Rita Vassena ,&nbsp;Gustavo Tiscornia","doi":"10.1016/j.rbmo.2024.103885","DOIUrl":"10.1016/j.rbmo.2024.103885","url":null,"abstract":"<div><h3>Research question</h3><div>What are the profiles of tubulin post-translational modifications (PTM) in the meiotic spindles of oocytes with different developmental competence: in-vivo matured MII oocytes (IVO) versus cumulus-free in-vitro matured germinal vesicles (IVM)?</div></div><div><h3>Design</h3><div>Cumulus-free germinal vesicles were matured <em>in vitro</em> and compared with IVO in terms of their tubulin PTM, characterizing tubulin PTM patterns (acetylation, tyrosination, detyrosination, D2-tubulin formation and polyglutamylation) of their meiotic spindles by immunofluorescence and by evaluation of the expression levels of the enzymes involved by quantitative reverse transcription polymerase chain reaction.</div></div><div><h3>Results</h3><div>Levels of D2-tubulin, tyrosination and polyglutamylation were similar in IVO and IVM oocytes; acetylation displayed different patterns in both groups, whereas detyrosination was detected only in MII oocytes matured <em>in vivo</em>. The PTM enzyme RNA expression levels in germinal vesicles, IVO and IVM were analysed, as well as in oocytes that failed to mature. Of the 24 PTM enzyme transcripts tested, 17 were present in at least one of the groups under study (<em>aΤΑΤ1, NAA50, HDAC6, SIRT2, TTL, TTLL1, TTLL2, TTLL4, TTLL5, TTLL6, TTLL9, TTLL11, TTLL12, CCP1, CCP5, CCP6, VASH1</em>), of which three were differentially expressed (<em>NAA50, TTLL12, CCP1</em>).</div></div><div><h3>Conclusion</h3><div>All modifications studied were present in human oocytes regardless of whether their meiotic maturation occurred <em>in vivo</em> or <em>in vitro</em>, except for detyrosination, which was found at low levels in the IVO group, but not in the IVM group. This suggests that the presence of tubulin PTMs may play a role in human oocyte maturation, completion of meiosis and developmental competence.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 103885"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139821182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The investigation and management of recurrent early pregnancy loss: a Canadian Fertility and Andrology Society clinical practice guideline","authors":"Sony Sierra ,&nbsp;Jason Min ,&nbsp;Julio Saumet ,&nbsp;Heather Shapiro ,&nbsp;Camille Sylvestre ,&nbsp;Jeff Roberts ,&nbsp;Kimberly Liu ,&nbsp;William Buckett ,&nbsp;Maria P. Velez ,&nbsp;Neal Mahutte","doi":"10.1016/j.rbmo.2024.104456","DOIUrl":"10.1016/j.rbmo.2024.104456","url":null,"abstract":"<div><div>This guideline defines recurrent early pregnancy loss (REPL) as two or more losses that occur before 10 weeks gestational age and includes non-consecutive and biochemical losses. Investigations should be considered on an individual basis and may include an evaluation of genetic, anatomical, endocrinological, structural and male-associated factors. Based on the findings and available resources, options for management may include preimplantation genetic testing (PGT) for aneuploidies or PGT for chromosomal structural rearrangements, progesterone supplementation and supportive care. This guideline emphasizes a personalized approach to the problem of REPL, recognizing an overall promising prognosis for this patient population and the avoidance of treatment options that have not been shown to be of benefit.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104456"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ICSI for non-male infertility: from ineffectiveness to gender bias?","authors":"Edgardo Somigliana ,&nbsp;Giussy Barbara ,&nbsp;Laila G. Micci ,&nbsp;Giorgia Di Stefano ,&nbsp;Alessio Paffoni ,&nbsp;Paola Vigano","doi":"10.1016/j.rbmo.2024.104706","DOIUrl":"10.1016/j.rbmo.2024.104706","url":null,"abstract":"<div><div>Intracytoplasmic sperm injection (ICSI) is currently being abused as it is also frequently employed in the absence of a frank male cause of infertility. There is growing and robust evidence showing that the use of ICSI should be limited to couples with male infertility. For all other causes of infertility, the procedure does not increase the chance of live birth, may increase malformations in newborns and requires more resources than conventional IVF. In addition, the use of ICSI may impact the sex ratio at birth, favouring female infants. Even if this effect may not be targeted as a gender biased procedure, it should be seen as a wake-up and warning call. It reminds us that ICSI is a ‘non-natural’ invasive technique that circumvents natural sperm selection mechanisms. Overall, a policy of indiscriminate use of ICSI is not justified and a plea is made for a return to a higher use of conventional IVF. Fear about failed fertilization or the use of preimplantation genetic testing for aneuploidies may represent barriers to the need to lower ICSI use and should be overcome.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104706"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Matter - Continued TOC","authors":"","doi":"10.1016/S1472-6483(25)00091-4","DOIUrl":"10.1016/S1472-6483(25)00091-4","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104884"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143641837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ‘Biological characteristics related to treatment effects of the levonorgestrel-releasing intrauterine system on adenomyosis-associated dysmenorrhoea’ Reproductive BioMedicine Online, 2024, 49;6:104393","authors":"Qiutong Li ,&nbsp;Dai Yi ,&nbsp;Xiaoyan Li ,&nbsp;Yushi Wu ,&nbsp;Zhiyue Gu ,&nbsp;Chenyu Zhang ,&nbsp;Hailan Yan ,&nbsp;Shiqing Lyu ,&nbsp;Biyun Zhang ,&nbsp;Jinghua Shi ,&nbsp;Jinhua Leng","doi":"10.1016/j.rbmo.2025.104829","DOIUrl":"10.1016/j.rbmo.2025.104829","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104829"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}