Reproductive biomedicine online最新文献

{"title":"Inside Front Cover - Affiliations and First page of TOC","authors":"","doi":"10.1016/S1472-6483(25)00204-4","DOIUrl":"10.1016/S1472-6483(25)00204-4","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 104997"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A digitally controlled, remotely operated ICSI system: case report of the first live birth","authors":"Gerardo Mendizabal-Ruiz ,&nbsp;Alejandro Chavez-Badiola ,&nbsp;Estefanía Hernández-Morales ,&nbsp;Roberto Valencia-Murillo ,&nbsp;Vladimir Ocegueda-Hernández ,&nbsp;Nuno Costa-Borges ,&nbsp;Enric Mestres ,&nbsp;Mónica Acacio ,&nbsp;Queralt Matia-Algué ,&nbsp;Adolfo Flores-Saiffe Farías ,&nbsp;David Salvador Martinez Carreon ,&nbsp;Carla Barragan ,&nbsp;Giuseppe Silvestri ,&nbsp;Amaranta Martinez-Alvarado ,&nbsp;Luis Miguel Campos Olmedo ,&nbsp;Aleska Valadez Aguilar ,&nbsp;Dante Josué Sánchez-González ,&nbsp;Alan Murray ,&nbsp;Mina Alikani ,&nbsp;Jacques Cohen","doi":"10.1016/j.rbmo.2025.104943","DOIUrl":"10.1016/j.rbmo.2025.104943","url":null,"abstract":"<div><h3>Research question</h3><div>Can a fully automated, digitally controlled, remotely operated system execute the entire micromanipulation process of intracytoplasmic sperm injection (ICSI)?</div></div><div><h3>Design</h3><div>A workstation automating the 23 micromanipulation steps of ICSI was engineered. The system operated independently under artificial intelligence control or under digital control executed by a remote operator. Pre-clinical validation was conducted with animal models to ensure safety and efficacy. In a clinical application, five donor oocytes intended for the treatment of a 40-year-old patient were fertilized using the automated ICSI system under remote supervision; three additional oocytes were injected manually as controls. Elective single embryo transfers were performed.</div></div><div><h3>Results</h3><div>The automated ICSI system achieved an 80% fertilization rate (<em>n</em> = 4/5), comparable with the manual controls (100%, <em>n</em> = 3/3). Two usable blastocysts were generated in each group. A fresh embryo transfer using an experimental embryo did not result in pregnancy. However, the transfer of a warmed blastocyst from the automated ICSI group resulted in a healthy live birth at 38 weeks of gestation. The system completed 49.6% of the required 115 micromanipulation steps (23 per oocyte) autonomously, and the remaining steps were conducted under digital control by the remote operator. The average time for automated injection of an oocyte was 9 min and 56 s. On-site human intervention was required for the initial set-up, and was needed on one occasion for troubleshooting one of the automated steps.</div></div><div><h3>Conclusions</h3><div>This study demonstrates the feasibility of fully automated, remotely operated ICSI achieving a live birth. While improvements in autonomy and efficiency are needed, this milestone highlights the potential for automated systems to transform assisted reproductive technology practices globally, enhancing accessibility and standardization.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 104943"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of mitochondrial DNA quantification in human blastocysts and assisted reproduction outcomes","authors":"Jing Zhang ,&nbsp;Fang Li ,&nbsp;Dan Kuai ,&nbsp;Haixia Chen,&nbsp;Xiaohuan Mu,&nbsp;Xueru Song,&nbsp;Yingmei Wang,&nbsp;Ye Yan,&nbsp;Ye Tian,&nbsp;Wenyan Tian,&nbsp;Xiaohong Bai","doi":"10.1016/j.rbmo.2024.104755","DOIUrl":"10.1016/j.rbmo.2024.104755","url":null,"abstract":"<div><h3>Research question</h3><div>Does the content of mitochondrial DNA (mtDNA) in human trophectoderm cells in blastocysts that received trophectoderm biopsy correlate with embryonic variables and the outcomes of assisted reproductive technology (ART)?</div></div><div><h3>Design</h3><div>To investigate whether the content of mtDNA in trophectoderm biopsies correlates with IVF outcome, 462 biopsies of blastocysts from 136 preimplantation genetic testing (PGT) cycles conducted between June 2022 and August 2024 were analysed. Euploid human blastocysts (<em>n</em> = 75) used in single frozen embryo transfer were studied. The mtDNA levels in trophectoderm cells were analysed by whole genome amplification and next-generation sequencing.</div></div><div><h3>Results</h3><div>Generalized linear regression analysis showed that only embryo euploidy was significantly associated with mitochondrial DNA copy number (MCN) (<em>P</em> &lt; 0.0001). Progesterone and LH concentration on HCG trigger day were not associated with MCN. Meanwhile, aneuploids had more mtDNA quantities than the euploids after correcting for blastocyst morphology. No statistically significant differences were found in the MCN and pregnancy outcomes (<em>P</em> = 0.619), and there were also no statistically significant differences were found when divided into high and low groups based on the median value or four groups at the quartiles.</div></div><div><h3>Conclusions</h3><div>Regardless of blastocyst morphology, euploid embryos had significantly fewer mtDNA copy numbers than aneuploid embryos. Nevertheless, pregnancy outcomes showed no statistically significant variations, suggesting that mtDNA copy number may not be a good predictor of optimal clinical success and high competence potential in IVF.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 104755"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elective co-parenting with someone already known versus someone met online: implications for parent and child psychological functioning","authors":"Sarah Foley ,&nbsp;Vasanti Jadva ,&nbsp;Susan Golombok","doi":"10.1016/j.rbmo.2024.104747","DOIUrl":"10.1016/j.rbmo.2024.104747","url":null,"abstract":"<div><h3>Research question</h3><div>What are the psychological outcomes for parents and children in elective co-parenting families, and do these differ based on whether or not the co-parents met online?</div></div><div><h3>Design</h3><div>This cross-sectional study provides novel descriptive quantitative data on the wellbeing of parents and children within 23 elective co-parenting families, defined as two or more parents deciding to have and raise children together outside of a romantic partnership or conjugal couple relationship. Standardized questionnaires were administered to assess parent and child psychological adjustment. Bayesian independent <em>t</em>-tests were conducted to compare the parent and child outcomes in 13 families who met online via a connection website with 10 families who were co-parenting with someone known to them previously.</div></div><div><h3>Results</h3><div>Elective co-parent scores for depression, anxiety, parenting stress, resilience, perceived social support and couple relationship satisfaction were within the normal range. Children's average competencies, and behavioural and emotional problem scores were low risk when compared with population norms. Bayes factors suggest no support for the alternative hypothesis that there were differences in parent or child wellbeing between families with co-parents who met via connection sites compared with families with co-parents who already knew each other.</div></div><div><h3>Conclusions</h3><div>Parents and children in elective co-parenting families are functioning well regardless of how they were formed, but individuals may require tailored professional advice or support for this growing new route to parenthood. Future longitudinal work with larger samples is required to replicate these findings, and explore children's perspectives of their families, as well as the support needs of co-parents and their children throughout their parenting journey.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 104747"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An explainable ultrasound-based machine learning model for predicting reproductive outcomes after frozen embryo transfer","authors":"Fangfang Xu ,&nbsp;Qianqing Ma ,&nbsp;Penghao Lai ,&nbsp;Lili Hu ,&nbsp;Chuanfen Gao ,&nbsp;Qianhua Xu ,&nbsp;Youyan Fang ,&nbsp;Yixuan Guo ,&nbsp;Wen Yao ,&nbsp;Chaoxue Zhang","doi":"10.1016/j.rbmo.2024.104743","DOIUrl":"10.1016/j.rbmo.2024.104743","url":null,"abstract":"<div><h3>Research question</h3><div>Can an optimal machine learning model be developed to predict reproductive outcomes following frozen embryo transfer (FET)?</div></div><div><h3>Design</h3><div>This prospective study included 787 infertile females who underwent FET. The participants were split into a training cohort (<em>n</em> = 550) and a test cohort (<em>n</em> = 237) at a ratio of seven to three. Radiomics features were extracted from ultrasound images of the endometrium and junctional zone. A radiomics model was developed to generate the radiomics score (rad score). Logistic regression was applied to process the clinical data and create a clinical model. A fusion machine learning model was developed by integrating the rad score with independent clinical data using the XGboost algorithm. The performance of the models was compared using the area under the receiver operating characteristic curve (AUC). The SHapley Additive exPlanations (SHAP) method was used to interpret and visualize the contributions of features to the outcomes of FET.</div></div><div><h3>Results</h3><div>The fusion model demonstrated superior performance, as indicated by an AUC of 0.861 (95% CI 0.829–0.890), in the training cohort, surpassing both the clinical model (AUC 0.680, 95% CI 0.635–0.722; <em>P</em> &lt; 0.001) and the radiomics model (AUC 0.814, 95% CI 0.777–0.848; <em>P</em> &lt; 0.001). The SHAP summary plot reveals the impacts of each feature on the predictive model, and the rad score was found to be the main feature. SHAP force plots provided explanations at the individual level.</div></div><div><h3>Conclusion</h3><div>An explainable machine learning model was established utilizing clinical data and ultrasound images to forecast the outcomes of FET. By utilizing the SHAP method, clinicians may better comprehend the contributors to the outcomes of FET in individual patients, and make better decisions before FET.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 104743"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of donor conception into identity and parental attachment: adolescents in heterosexual-couple and lesbian-couple families","authors":"Henrik Groundstroem ,&nbsp;Johan Paulin ,&nbsp;Gunilla Sydsjö ,&nbsp;Claudia Lampic","doi":"10.1016/j.rbmo.2024.104758","DOIUrl":"10.1016/j.rbmo.2024.104758","url":null,"abstract":"<div><h3>Research question</h3><div>Is parental attachment related to the integration of donor conception into identity among adolescents in heterosexual-couple and lesbian-couple families following identity-release oocyte and sperm donation?</div></div><div><h3>Design</h3><div>A cross-sectional survey was conducted in 2022–2023 as part of a longitudinal study on two-parent families with donor-conceived children. Of 128 donor-conceived adolescents approached, 100 (78% response rate) participated. Adolescents’ attachment to their parents was measured with the validated Inventory of Parent and Peer Attachment instrument, and integration of donor conception into identity with the Donor Conception Identity Questionnaire subscales Curiosity and Avoidance. Data analyses were performed with multiple linear regression analyses.</div></div><div><h3>Results</h3><div>Adolescents with more secure ratings of attachment to their father/co-mother were less curious about their donor origin (<em>P = </em>0.010). Although showing a rather small effect (<em>R</em>² = 0.073), the significant relationship remained when controlling for donation group, gender and age. No relationship was found concerning attachment to the birth mother (<em>P = </em>0.125). Parental attachment was not related to feelings of avoidance with regards to their donor conception. Overall, the adolescents reported high ratings of attachment security to both parents and relatively low ratings of curiosity and avoidant feelings concerning the donor conception.</div></div><div><h3>Conclusion</h3><div>Among adolescents in heterosexual-couple and lesbian-couple families, attachment to the co-parent rather than the birth mother appears influential for their curiosity about the donor conception, which points to the psychological complexities of donor conception in families with potential access to donor information.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 104758"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin activates SIRT2 to improve insulin resistance and promote granulosa cell glycolysis in a rat model of polycystic ovary syndrome","authors":"Zhihan Zhang ,&nbsp;Peng Huo ,&nbsp;Xiaocan Lei ,&nbsp;Haoxuan Xue ,&nbsp;Xiuli Yang ,&nbsp;Jianghua Le ,&nbsp;Shun Zhang","doi":"10.1016/j.rbmo.2024.104750","DOIUrl":"10.1016/j.rbmo.2024.104750","url":null,"abstract":"<div><h3>Research question</h3><div>What is the mechanism by which metformin enhances insulin sensitivity, improves granulosa cell glycolysis and induces ovulation in a rat model of polycystic ovary syndrome (PCOS)?</div></div><div><h3>Design</h3><div>Analysis of the GSE168404 gene expression profile in the Gene Expression Omnibus database revealed increased levels of <em>IGF1</em> and decreased levels of glycolytic enzymes (<em>HK2, LDHA, PKM2</em>) in the granulosa cells of PCOS patients. To explore the effects of metformin on the imbalance in glycolysis induced by insulin resistance (IR), experiments were conducted using Sprague–Dawley rats and KGN cells (human ovarian granulosa cells). Oestrous cycles were monitored in control, PCOS model (induced by letrozole and a high-fat diet) and metformin-treated PCOS groups. Analyses of body weight, hormone concentrations and biochemical, histopathological, immunohistochemical and glycolytic pathways were performed. KGN cells were used to model insulin resistance with insulin, and AGK2 was used specifically to inhibit sirtuin 2 (SIRT2), while metformin was applied.</div></div><div><h3>Results</h3><div>Metformin significantly improved insulin resistance in PCOS rats, reduced insulin-like growth factor 1 (IGF1) protein and mRNA expression (all <em>P</em> ≤ 0.0348) and increased IGF1 receptor (IGF1R) impression (all <em>P</em> ≤ 0.0361). Insulin inhibited glycolytic activity in KGN cells, but metformin attenuated this effect (all <em>P</em> ≤ 0.0255). Metformin reversed the inhibition of SIRT2 in PCOS rat ovaries (all <em>P</em> ≤ 0.0483) and restored glycolysis in KGN cells treated by AGK2 (all <em>P</em> ≤ 0.0369).</div></div><div><h3>Conclusion</h3><div>Metformin enhances insulin sensitivity and restores glycolysis by regulating SIRT2, which may improve follicular development and reduce ovarian damage in PCOS rats, offering a potential clinical treatment strategy for PCOS.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 104750"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to ‘A precise and cost-efficient whole-genome haplotyping method without probands: preimplantation genetic testing analysis’ Reproductive BioMedicine Online 50/1 (2025) 104328","authors":"Zhiqiang Zhang ,&nbsp;Kai Kang ,&nbsp;Linan Xu ,&nbsp;Xiaolan Li ,&nbsp;Shujing He ,&nbsp;Ruixia Xu ,&nbsp;Lei Jia ,&nbsp;Shihui Zhang ,&nbsp;Wenlong Su ,&nbsp;Peng Sun ,&nbsp;Mengnan Gu ,&nbsp;Wenqi Shan ,&nbsp;Yawen Zhang ,&nbsp;Lingyin Kong ,&nbsp;Bo Liang ,&nbsp;Cong Fang ,&nbsp;Zi Ren","doi":"10.1016/j.rbmo.2025.104952","DOIUrl":"10.1016/j.rbmo.2025.104952","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 104952"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenomyosis-associated infertility: an update of the immunological perspective","authors":"Ying Cao ,&nbsp;Dongyong Yang ,&nbsp;Songchen Cai ,&nbsp;Lingtao Yang ,&nbsp;Shuyi Yu ,&nbsp;Qiang Geng ,&nbsp;Meilan Mo ,&nbsp;Wenzhu Li ,&nbsp;Yiqiu Wei ,&nbsp;Yuye Li ,&nbsp;Tailang Yin ,&nbsp;Lianghui Diao","doi":"10.1016/j.rbmo.2024.104703","DOIUrl":"10.1016/j.rbmo.2024.104703","url":null,"abstract":"<div><div>Adenomyosis is characterized by the invasion of endometrial glands and stroma into the myometrium. Its clinical manifestations often include dysmenorrhoea, excessive menstrual bleeding and infertility. Reduced pregnancy and live birth rates and an increased miscarriage rate are observed in women with adenomyosis. This review summarizes relevant advances and presents the underlying mechanisms of adenomyosis-associated infertility from an immunological perspective. Individuals with adenomyosis exhibit imbalances in immune cell subpopulations and the endocrine hormone–immunomodulatory axis. These immunological alterations may be key contributors to, or at least accomplices in, impaired endometrial receptivity. In addition, adenomyosis often occurs in association with endometriosis, uterine leiomyoma or endometrial polyps, which are pathogenetically relevant; their similarities and differences are discussed from an immunological perspective. The clinical diagnostic criteria of adenomyosis are not perfect, and the pathogenesis remains to be fully explored. Therefore screening for effective targets for early diagnosis and treatment at the cellular and molecular levels from the immunological point of view holds great potential, which will be of great importance in preventing this disease and improving women's reproductive health.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 104703"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Editorial Board","authors":"","doi":"10.1016/S1472-6483(25)00217-2","DOIUrl":"10.1016/S1472-6483(25)00217-2","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 105010"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}