Reproductive biomedicine online最新文献

{"title":"Comparing subjective indicators of health in adults aged 27–38 years conceived with and without assisted reproductive technology","authors":"Sharon Lewis ,&nbsp;Karin Hammarberg ,&nbsp;Joanne Kennedy ,&nbsp;Sarah Biggs ,&nbsp;David J. Amor ,&nbsp;Anne Glynn ,&nbsp;Martha Hickey ,&nbsp;John McBain ,&nbsp;Jane Halliday","doi":"10.1016/j.rbmo.2024.104777","DOIUrl":"10.1016/j.rbmo.2024.104777","url":null,"abstract":"<div><h3>Research question</h3><div>What are the health outcomes of individuals aged 27–38 years conceived with and without assisted reproductive technology (ART)?</div></div><div><h3>Design</h3><div>An online survey, hosted in REDCap, was used to collect information on self-reported physical, psychological and reproductive health from an established cohort of people conceived with and without ART who were now aged 27–38 years. Questions included lists of common conditions and required tick-box responses to determine prevalence. A validated scale was used to assess psychological well-being.</div></div><div><h3>Results</h3><div>There were 313 ART-conceived (236 IVF and 77 gamete intrafallopian transfer [GIFT]) and 153 non-ART conceived respondents. No marked differences between the ART and non-ART groups were observed in physical or psychological health measures. Similar proportions in both groups had had children and reported using medically assisted reproduction to conceive. More women in the ART than the non-ART group reported at least one reproductive health disorder (P = 0.01). Male reproductive health disorders were rare in both groups.</div></div><div><h3>Conclusions</h3><div>Subjective indicators of health were no different between ART- and non-ART-conceived individuals, except for a slight increase in adverse reproductive health disorders reported by ART-conceived female participants. This warrants closer clinical scrutiny. Overall, a larger sample and objective measures in individuals beyond 30 years of age would provide the ultimate reassurance that ART is not associated with long-term health problems. This study is the first of its kind and may provide some reassurance of safety for people conceived with ART and those who contemplate using it.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 6","pages":"Article 104777"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Higher CD16-NKp46bright uterine endometrial natural killer cells may predict pregnancy success in women experiencing recurrent reproductive failure","authors":"Chuxian Mai ,&nbsp;Mayu Yamamoto ,&nbsp;Hiroaki Shibahara ,&nbsp;Qiong Wang ,&nbsp;Atsushi Fukui","doi":"10.1016/j.rbmo.2024.104756","DOIUrl":"10.1016/j.rbmo.2024.104756","url":null,"abstract":"<div><h3>Research question</h3><div>Could CD16^-NKp46^bright uterine endometrial natural killer (uNK) cells serve as a predictor of pregnancy success in women with unknown recurrent reproductive failure (URRF), and what are the underlying mechanisms involved?</div></div><div><h3>Design</h3><div>A prospective study involving 63 women with URRF, followed up for 2 years. After age adjustment, 17 women remained in both the pregnant and non-pregnant groups. The pregnant group was further divided into the live birth (<em>n</em> = 10) and miscarriage (<em>n</em> = 4) groups, with three women lost to follow-up. Surface antigens expressed and cytokine produced in uNK cells were analysed with multicolour flow cytometry.</div></div><div><h3>Results</h3><div>Expression NKp46⁺ uNK (<em>P</em> = 0.034), NKp46^bright uNK (<em>P</em> = 0.045), CD16^-NKp46^bright uNK (<em>P</em> = 0.026), NKp46^brightNKG2D⁺ uNK (<em>P</em> = 0.004) and NKp46⁺NKG2D⁺ uNK (<em>P</em> = 0.037) cells was significantly lower in the non-pregnant group compared with the pregnant group. Also, the expression of CD16^-NKp46^bright uNK cells was significantly (<em>P</em> = 0.040) higher in the live birth group compared with the non-pregnant group. The threshold 44.9% of CD16^-NKp46^bright uNK cells showed the largest area under the curve. Women with decreased CD16^-NKp46^bright uNK cells (&lt;44.9%), produced significantly higher TNF-α⁺IFN-γ⁺ in CD56⁺ uNK (<em>P</em> = 0.014) and in CD56^bright uNK cells (<em>P</em> = 0.013) and significantly lower TNF-α^-IFN-γ^- in CD56⁺ uNK (<em>P</em> = 0.039) and in CD56^bright uNK cells (<em>P</em> = 0.017), and had an elevated risk of failing to achieve live birth or pregnancy (OR 21.60, 95% CI 2.14 to 218.58; <em>P</em> = 0.004 (OR 11.20, 95% CI 2.20 to 56.93; <em>P</em> = 0.005).</div></div><div><h3>Conclusions</h3><div>CD16^-NKp46^bright uNK cells are a protective factor as well as an appropriate candidate for predicting pregnancy success in URRF.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 6","pages":"Article 104756"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Live birth rates in IVF patients with and without polycystic ovary syndrome according to serum anti-Müllerian hormone concentrations","authors":"Tongjie Li ,&nbsp;Huiling An ,&nbsp;Guidong Yao ,&nbsp;Wei Dai ,&nbsp;Ruizhe Zhang ,&nbsp;Ting Chu ,&nbsp;Jun Zhai","doi":"10.1016/j.rbmo.2024.104731","DOIUrl":"10.1016/j.rbmo.2024.104731","url":null,"abstract":"<div><h3>Research question</h3><div>Is there a relationship between serum anti-Müllerian hormone (AMH) concentration and live birth rate (LBR) in patients with and without polycystic ovary syndrome (PCOS)?</div></div><div><h3>Design</h3><div>This retrospective study included 4631 patients aged 20–35 years who underwent their first frozen embryo transfer cycle at the First Affiliated Hospital of Zhengzhou University from June 2016 to June 2021. Participants were categorized as having or not having PCOS (PCOS group, <em>n</em> = 1428, and NPCOS group, <em>n</em> = 3203, respectively). In the two groups, AMH was categorized into subgroups 1, 2 and 3 using the 25th and 75th percentiles as cut-off lines.</div></div><div><h3>Results</h3><div>In the NPCOS subgroups, LBR was significantly lower in NPCOS1 than in NPCOS2 and NPCOS3 (<em>P</em> &lt; 0.001). In the PCOS subgroups, LBR was significantly lower in PCOS3 than in PCOS2 (<em>P</em> = 0.038), with no significant difference between PCOS1 and the other subgroups. In the NPCOS and PCOS groups, the preterm birth rate increased with the increase in AMH concentration in the subgroups (<em>P</em> = 0.018 and <em>P</em> = 0.016, respectively). Multivariate regression analysis revealed that AMH was an independent factor influencing the LBR and preterm birth rate in the NPCOS and PCOS groups (<em>P</em> &lt; 0.05). The threshold effect analysis indicated a positive correlation between LBR and AMH in the NPCOS group at an AMH concentration of 2.98 ng/ml or less (<em>P</em> &lt; 0.001), and a negative correlation in the PCOS group at an AMH concentration higher than 7.13 ng/ml (<em>P</em> = 0.043). Higher AMH concentrations were positively correlated with preterm birth rate in the NPCOS (<em>P</em> = 0.004) and PCOS (<em>P</em> = 0.017) groups.</div></div><div><h3>Conclusions</h3><div>Serum AMH concentrations could have a clinical predictive value for LBR in young patients, both with and without PCOS.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 6","pages":"Article 104731"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Istanbul Consensus update: a revised ESHRE/ALPHA consensus on oocyte and embryo static and dynamic morphological assessment† ‡","authors":"The Working Group on the update of the ESHRE/ALPHA Istanbul Consensus,,&nbsp;Giovanni Coticchio ,&nbsp;Aisling Ahlström ,&nbsp;Gemma Arroyo ,&nbsp;Basak Balaban ,&nbsp;Alison Campbell ,&nbsp;Maria José De Los Santos ,&nbsp;Thomas Ebner ,&nbsp;David K. Gardner ,&nbsp;Borut Kovačič ,&nbsp;Kersti Lundin ,&nbsp;M. Cristina Magli ,&nbsp;Saria Mcheik ,&nbsp;Dean E. Morbeck ,&nbsp;Laura Rienzi ,&nbsp;Ioannis Sfontouris ,&nbsp;Nathalie Vermeulen ,&nbsp;Mina Alikani","doi":"10.1016/j.rbmo.2025.104955","DOIUrl":"10.1016/j.rbmo.2025.104955","url":null,"abstract":"<div><div>This European Society of Human Reproduction and Embryology (ESHRE)/Alpha Scientists in Reproductive Medicine (ALPHA) consensus document provides several novel recommendations to assess oocyte and embryo morphology and rank embryos for transfer. A previous ALPHA/ESHRE consensus on oocyte and embryo morphological assessment was published in 2011. After more than a decade, and the integration of time-lapse technology into embryo culture and assessment, a thorough review and update was needed. A working group consisting of ALPHA members and ESHRE Special interest group of Embryology members formulated recommendations on oocyte and embryo assessment. The working group included 17 internationally recognized experts with extensive experience in clinical embryology. Seven members represented ALPHA and eight members represented ESHRE, along with two methodological experts from the ESHRE central office. Based on a systematic literature search and discussion of existing evidence, the recommendations of the Istanbul Consensus (2011) were reassessed and, where appropriate, updated based on consensus within the working group. A stakeholder review was organized after the updated draft was finalized. The final version was approved by the working group, the ALPHA Executive Committee and the ESHRE Executive Committee. This updated consensus paper provides 20 recommendations focused on the timeline of preimplantation developmental events and morphological criteria for oocyte, zygote and embryo assessment. Based on the duration of embryo culture, recommendations are given on the frequency and timing of assessments to ensure consistency and effectiveness. Several criteria relevant to oocyte and embryo morphology have not been well studied, leading to either a recommendation against their use for grading or for their use in ranking rather than grading. Future updates may require further revision of these recommendations. This document provides embryologists with advice on best practices when assessing oocyte and embryo quality based on the most recent evidence.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 6","pages":"Article 104955"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the impact of isthmocele and its surgical repair on fertility: results from a systematic review and meta-analysis","authors":"Andrea Busnelli ,&nbsp;Paolo E. Levi-Setti ,&nbsp;Annalisa Inversetti ,&nbsp;Tommaso Bignardi ,&nbsp;Amerigo Vitagliano ,&nbsp;Cristian Dell’Acqua ,&nbsp;Enrica Zambella ,&nbsp;Nicoletta Di Simone","doi":"10.1016/j.rbmo.2024.104746","DOIUrl":"10.1016/j.rbmo.2024.104746","url":null,"abstract":"<div><div>The aim of this systematic review was to quantify the impact of isthmocele and its treatment on fertility. The review was conducted according to the PRISMA guidelines, and the meta-analysis according to the MOOSE guidelines. The meta-analysis of data from 29 studies showed that 43% (rate 0.43, 95% CI 0.33–0.54; <em>n</em> = 2038) of women with a symptomatic isthmocele reported secondary infertility as a presenting symptom (quality of evidence [QOE)]: very low). In the IVF setting, women with an isthmocele were shown to have a lower live birth rate (LBR) than women with a previous Caesarean section without an isthmocele (odds ratio [OR] 0.57, 95% CI 0.49–0.66; <em>n</em> = 7719, eight studies) (QOE: high). Ten studies reported the LBR after isthmocele hysteroscopic repair in a cohort of infertile women (LBR 0.63, 95% CI 0.49–0.77; QOE: very low). Seven studies reported the LBR after isthmocele laparoscopic repair (LBR 0.51, 95% CI 0.40–0.63; QOE: very low). The combination of these findings suggests that isthmoceles play a role in the pathophysiology of infertility. Hysteroscopic and laparoscopic surgical repair emerged as promising options for the treatment of infertility in women with an isthmocele. To provide comprehensive counselling, these findings must be communicated to patients, together with the weaknesses of the underlying evidence.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 6","pages":"Article 104746"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hysteroscopic resection as primary management in case of early pregnancy loss: time for a paradigm shift?","authors":"Michelle Soares ,&nbsp;Liese Boudry ,&nbsp;Letizia Li Piani ,&nbsp;Kim van Berkel ,&nbsp;Herman Tournaye ,&nbsp;Valerie Uvin ,&nbsp;Michael De Brucker ,&nbsp;Shari Mackens ,&nbsp;Christophe Blockeel","doi":"10.1016/j.rbmo.2024.104745","DOIUrl":"10.1016/j.rbmo.2024.104745","url":null,"abstract":"<div><h3>Research question</h3><div>What is the potential role of hysteroscopic resection (HsR) of early pregnancy loss (EPL) according to its feasibility and efficacy, including in the outpatient setting, for early-stage pregnancies?</div></div><div><h3>Design</h3><div>Retrospective cohort study and literature review. Patients undergoing HsR of EPL in the authors’ tertiary fertility centre between November 2022 and January 2024 were included. Patients with gestational sacs &lt;20 mm could choose local anaesthesia; patients with larger gestational sacs were managed under sedation. Feasibility, tolerability and complications were evaluated. Postoperative retained products of conception (RPOC) were assessed after the first menstrual period. Gestational tissue was analysed for aneuploidy using chromosomal microarray analysis.</div></div><div><h3>Result</h3><div>Thirty-six patients were included, with a mean ± SD age of 36.7 ± 4.1 years. Mean gestational age and gestational sac size were 9 + 1/7 weeks and 27.5 mm, respectively. Mean procedure time was 17.7 min, with no peri- or postoperative complications. Outpatient procedures (<em>n</em> = 10) were well tolerated, with one requiring conversion to sedation. One (1/36, 2.8%) patient had postoperative RPOC. Chromosomal analysis was performed in 32 patients and was conclusive in 26 (81.3%) cases; 53.8% of cases were chromosomically abnormal.</div></div><div><h3>Conclusion</h3><div>HsR of EPL is feasible and safe, with low incidence of RPOC and reliable genetic results. It can be performed under general or local anaesthesia based on the size of the gestational sac. HsR may be a preferable alternative to aspiration curettage, especially for patients with recurrent EPL or risk of intrauterine adhesions. Further comparative studies are warranted to support this paradigm shift.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 6","pages":"Article 104745"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Editorial Board","authors":"","doi":"10.1016/S1472-6483(25)00246-9","DOIUrl":"10.1016/S1472-6483(25)00246-9","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 6","pages":"Article 105039"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subtle translocation does not affect euploid blastocyst rate or pregnancy outcomes during preimplantation genetic testing","authors":"Yun Wang ,&nbsp;Yuchao Zheng ,&nbsp;Yize Han ,&nbsp;Minjie Shao ,&nbsp;Liang Chang ,&nbsp;Jin Huang","doi":"10.1016/j.rbmo.2025.104806","DOIUrl":"10.1016/j.rbmo.2025.104806","url":null,"abstract":"<div><h3>Research question</h3><div>Do chromosomal translocation segment size and proportion affect embryo euploidy and pregnancy outcomes during preimplantation genetic testing (PGT) cycles in subtle translocation carriers?</div></div><div><h3>Design</h3><div>Retrospective cohort study including 207 couples with reciprocal translocation who underwent 232 PGT for structural rearrangements cycles; euploid blastocysts were selected for transfer. A total of 119 couples completed 155 warming transfer cycles, and 58 cycles resulted in clinical pregnancy. According to the size of the translocation segment (threshold 10 Mb), couples were divided into subtle translocation group 1 (translocation segment on either chromosome was &lt;10 Mb) and control group 1 (translocation segments on both chromosomes were ≥10 Mb). Couples were divided into subtle translocation group 2 (proportion on either chromosome segment was &lt;5% relative to the whole chromosome) and control group 2 (proportions on both chromosome segments were ≥5% relative to the whole chromosome). Rates of euploid blastocysts and clinical outcomes were analysed.</div></div><div><h3>Results</h3><div>No significant differences were found in the rates of euploid blastocysts, (37.11% versus 36.08%; <em>P</em> = 0.817) and clinical pregnancy (32.56% versus 39.28%; <em>P</em> = 0.438) compared with subtle translocation group 1 and control group 1, respectively. Similarly, no significant differences were found in the rates of euploid blastocysts (35.00% versus 36.75%; <em>P</em> = 0.572) and clinical pregnancy (34.28% versus 38.33%; <em>P</em> = 0.663) compared with subtle translocation group 2 and control group 2.</div></div><div><h3>Conclusions</h3><div>The size and proportion of a translocation segment did not significantly affect the rate of euploid blastocysts or pregnancy outcomes during PGT cycles for couples with chromosomal translocations.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 6","pages":"Article 104806"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the health outcomes of ART-conceived adults: reassurance and future directions","authors":"Laxmi Shingshetty","doi":"10.1016/j.rbmo.2025.104983","DOIUrl":"10.1016/j.rbmo.2025.104983","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 6","pages":"Article 104983"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ‘Effect of overweight and obesity on live birth rate in single euploid frozen embryo transfers’ Reproductive BioMedicine Online 50(3) (2025) 104443","authors":"Francisco Ruiz ,&nbsp;Barbara Lawrenz ,&nbsp;Erkan Kalafat ,&nbsp;Baris Ata ,&nbsp;Alberto Linan ,&nbsp;Ibrahim Elkhatib ,&nbsp;Laura Melado ,&nbsp;Human Fatemi","doi":"10.1016/j.rbmo.2025.105014","DOIUrl":"10.1016/j.rbmo.2025.105014","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 6","pages":"Article 105014"},"PeriodicalIF":3.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}