Reproductive biomedicine online最新文献

{"title":"Adipokines level in plasma, hypothalamus, ovaries and adipose tissue of rats with polycystic ovary syndrome","authors":"Karolina Pich ,&nbsp;Klaudia Pietroń ,&nbsp;Agata Szlaga ,&nbsp;Maria Billert ,&nbsp;Marek Skrzypski ,&nbsp;Piotr Pawlicki ,&nbsp;Małgorzata Kotula-Balak ,&nbsp;Joëlle Dupont ,&nbsp;Anna Błasiak ,&nbsp;Agnieszka Rak","doi":"10.1016/j.rbmo.2024.104693","DOIUrl":"10.1016/j.rbmo.2024.104693","url":null,"abstract":"<div><h3>Research question</h3><div>Do the levels of adipokines (adiponectin, apelin, chemerin and vaspin) in plasma, hypothalamus, ovaries and periovarian adipose tissue differ during polycystic ovarian syndrome (PCOS)?</div></div><div><h3>Design</h3><div>The PCOS was induced in rats by oral administration of non-steroidal aromatase inhibitor letrozole. To determine the plasma levels of adiponectin, apelin, chemerin and vaspin enzyme-linked immunosorbent assays were carried out. To assess the expression (gene and protein) and immunolocalization of these adipokines and their receptors, namely Adipor1 and Adipor2 for adiponectin, Aplnr for apelin, Ccrl2, Cmklr1 and Gpr1 for chemerin and Grp78 for vaspin in the hypothalamus, real-time polymerase chain reaction, and Western blot and immunohistochemistry were used to analyse ovaries and periovarian adipose tissue respectively.</div></div><div><h3>Results</h3><div>In PCOS, the plasma level of adiponectin decreased (<em>P</em> = 0.0003), whereas apelin, chemerin and vaspin increased (<em>P</em> ≤ 0.0479). Moreover, PCOS modulates the expression of adipokines and their receptors in the hypothalamus, ovaries and periovarian adipose tissue compared with healthy rats (<em>P</em> ≤ 0.487).</div></div><div><h3>Conclusions</h3><div>A strong relationship was found between PCOS and adipokines, which suggests that adipokines may be a biomarker of PCOS.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 104693"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of unequal-sized pronuclei and their origin on embryo development and obstetric outcomes: a time-lapse retrospective study","authors":"Tsubasa Takahashi,&nbsp;Kenji Ezoe,&nbsp;Mai Mogi,&nbsp;Hikari Akaike,&nbsp;Ayano Sawado,&nbsp;Ayumi Amagai,&nbsp;Tetsuya Miki,&nbsp;Satoshi Ueno,&nbsp;Kiyoe Shimazaki,&nbsp;Tadashi Okimura,&nbsp;Keiichi Kato","doi":"10.1016/j.rbmo.2024.104701","DOIUrl":"10.1016/j.rbmo.2024.104701","url":null,"abstract":"<div><h3>Research question</h3><div>Do maternal and paternal pronuclear sizes and their relative differences affect embryonic development, morphokinetics and pregnancy outcomes in human embryos?</div></div><div><h3>Design</h3><div>A total of 2516 fertilized oocytes with two pronuclei from 1207 patients were assessed using a time-lapse culture system. The associations between the pronuclear area immediately before pronuclear breakdown and its relative ratio (PNR), and embryonic, pregnancy and perinatal outcomes, were retrospectively evaluated. Perinatal outcomes were obtained from a self-reported questionnaire. Zygotes were stratified by PNR and origin of the pronuclei; embryo development, morphokinetics and morphological alterations were compared among the zygotes.</div></div><div><h3>Results</h3><div>Areas of maternal and paternal pronuclei were not correlated with embryonic, pregnancy and perinatal outcomes. Zygotes with a PNR lower than the median (&lt;0.88, unequal-sized pronuclei) had impaired embryo development (expanded blastocyst; <em>P</em> = 0.0100). Unequal-sized pronuclei resulted in a prolonged time interval between maternal and paternal pronuclear appearance, decreased nucleolus precursor body (NPB) alignment and increased incidence of asynchronous pronuclear breakdown, asymmetric division and multinucleation (<em>P</em> &lt; 0.0001–0.0230). When the paternal pronucleus was smaller than the maternal pronucleus, the decreased NPB alignment, asynchronous pronuclear breakdown and abnormal cleavage were observed more frequently, resulting in significantly decreased blastocyst formation compared with the zygotes with equal-sized pronuclei (<em>P</em> &lt; 0.0001–0.0030).</div></div><div><h3>Conclusions</h3><div>Zygotes with unequal-sized pronuclei had impairments in preimplantation development, particularly when the paternal pronucleus was smaller than the maternal pronucleus, without any adverse effects on maternal and obstetric outcomes. In addition to the number of pronuclei, evaluating PNR and pronuclear origin would be beneficial when fertilization is verified.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 5","pages":"Article 104701"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenomyosis: the missed disease","authors":"Pietro Santulli ,&nbsp;Silvia Vannuccini ,&nbsp;Mathilde Bourdon ,&nbsp;Charles Chapron ,&nbsp;Felice Petraglia","doi":"10.1016/j.rbmo.2025.104837","DOIUrl":"10.1016/j.rbmo.2025.104837","url":null,"abstract":"<div><div>Adenomyosis, a menstruation-related uterine disorder, refers to the presence of endometrial stroma and glands within the myometrium and is typically observed in reproductive-age women. The pathogenesis explaining the migration, persistence, proliferation and differentiation of ectopic endometrial cells includes a genetic and epigenetic background, an oestrogen/progesterone receptor imbalance and an inflammatory reaction driven by local immune dysfunction, along with fibrosis and neuroangiogenesis within the myometrium. In the past, it was thought that adenomyosis almost exclusively affected multiparous women after 40 years of age and the diagnosis was generally confirmed upon hysterectomy. Nowadays, using imaging techniques such as transvaginal ultrasonography and magnetic resonance imaging, adenomyosis is increasingly identified in young women with dysmenorrhoea, dyspareunia, abnormal uterine bleeding and heavy menstrual bleeding, and also in infertile patients. Furthermore, adenomyosis often coexists with other gynaecological conditions, such as endometriosis and uterine fibroids. Despite the improvement of non-invasive diagnostic tools, the awareness of the condition is still poor and the diagnosis is often missed, due also to a heterogeneity in clinical presentation and imaging criteria. In addition, medical and surgical management do not follow shared recommendations, even though adenomyosis requires a lifelong management plan, including pain and bleeding control, fertility preservation and pregnancy complications.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104837"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is planned oocyte cryopreservation delivering?","authors":"Ana Cobo ,&nbsp;Sarah Druckenmiller Cascante ,&nbsp;Juan García-Velasco ,&nbsp;James A. Grifo","doi":"10.1016/j.rbmo.2025.104794","DOIUrl":"10.1016/j.rbmo.2025.104794","url":null,"abstract":"<div><div>The objective of this review is to determine whether planned oocyte cryopreservation is successfully providing women with reproductive autonomy and the opportunity to shape their families. Planned oocyte cryopreservation is an established means to expand the reproductive function of oocytes and is not associated with an increased risk of congenital anomalies or short-term health risks to the offspring. There is sufficient clinical evidence to support the success of planned oocyte cryopreservation; however, this technology does not guarantee live birth, and outcomes greatly depend on both the age at cryopreservation and the total number of cryopreserved oocytes. While reproducibility between centres must be improved, the results from the authors two large, experienced centres are consistent and provide useful data for patient counselling. Planned oocyte cryopreservation provides the highest cumulative live birth rates (&gt;75%) when it is performed below the age of 35 years and 15–20 or more mature oocytes are cryopreserved. Live birth rates from planned oocyte cryopreservation at an ideal age are higher than live birth rates from women who delay childbearing past their reproductive prime and then attempt natural conception followed by IVF if they are unsuccessful.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104794"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence in assisted reproductive technology: separating the dream from reality","authors":"Jacques Cohen ,&nbsp;Giuseppe Silvestri ,&nbsp;Omar Paredes ,&nbsp;Hector E. Martin-Alcala ,&nbsp;Alejandro Chavez-Badiola ,&nbsp;Mina Alikani ,&nbsp;Giles A. Palmer","doi":"10.1016/j.rbmo.2025.104855","DOIUrl":"10.1016/j.rbmo.2025.104855","url":null,"abstract":"<div><div>This paper critically reviews the role of artificial intelligence (AI) in assisted reproductive technology (ART), a nascent field that has emerged over the last decade. While AI holds immense promise for enhancing IVF efficiency, standardization, and outcomes, its current trajectory reveals significant challenges. Much of the recent literature presents variations on established methodologies rather than groundbreaking advancements, with many studies lacking clear clinical applications or outcome-driven validations. Moreover, the growing enthusiasm for AI in ART is often accompanied by undue hype that obscures its realistic potential and fosters inflated expectations. Despite these limitations, AI-driven innovations such as advanced image analysis, personalized protocols, and automation of embryology workflows are beginning to show value. Machine learning algorithms and robotics may help address inefficiencies, alleviate staff shortages, and improve decision-making in the IVF laboratory. However, progress is tempered by drawbacks including ethical concerns, limited transparency in AI systems, and regulatory impediments. Data-sharing barriers in our field hinder AI tool development significantly. Energy-intensive computational processes and expanding data centers also raise sustainability concerns, underscoring the need for environmentally responsible development. As the field evolves, it must emphasize rigorous validation, collaborative data frameworks, and alignment with the needs of ART practitioners and patients.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104855"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sir Robert Edwards: Founder of RBMO","authors":"","doi":"10.1016/j.rbmo.2025.104881","DOIUrl":"10.1016/j.rbmo.2025.104881","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104881"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"25 years of advancing the frontiers of reproductive medicine","authors":"Nick Macklon,&nbsp;Juan Garcia-Velasco","doi":"10.1016/j.rbmo.2025.104863","DOIUrl":"10.1016/j.rbmo.2025.104863","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104863"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Matter - Continued TOC","authors":"","doi":"10.1016/S1472-6483(25)00117-8","DOIUrl":"10.1016/S1472-6483(25)00117-8","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104910"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renewing ovarian stimulation","authors":"Baris Ata","doi":"10.1016/j.rbmo.2024.104788","DOIUrl":"10.1016/j.rbmo.2024.104788","url":null,"abstract":"<div><div>Conventional ovarian stimulation protocols were designed for fresh embryo transfer. They start in the early follicular phase, i.e. the infamous day 2-3, gonadotrophin-releasing hormone analogues are used to prevent premature ovulation, and the stimulation is harnessed due to the fear of ovarian hyperstimulation syndrome and the decreasing chance of a live birth following a fresh transfer. The advent of vitrification allows a confident cancellation of fresh transfers and effective oocyte/embryo freezing for future use. Today, many stimulation cycles are not intended to involve a fresh embryo transfer. Ovarian stimulation for freeze-all cycles can be vastly different from conventional stimulation. The quantitative and qualitative oocyte yield seems independent of the starting day, and the early or late follicular or luteal phase may have distinct advantages for different needs. A variety of pituitary suppression protocols including oral progestins or not using exogenous suppression at all can be employed without compromising oocyte quantity and quality. Stimulation can cautiously aim for the patient's maximal potential since the agonist trigger without a fresh transfer curbs the risk of ovarian hyperstimulation syndrome. Patients can be even stimulated multiple times in a menstrual cycle when deemed beneficial. Ovarian stimulation is more patient and physician friendly than ever.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104788"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The road from Duck End Farm: the formative years of RBMOnline","authors":"Kamal K. Ahuja ,&nbsp;Simon Brown","doi":"10.1016/j.rbmo.2025.104853","DOIUrl":"10.1016/j.rbmo.2025.104853","url":null,"abstract":"<div><div><em>Reproductive BioMedicine Online</em> has now reached its 25th year and in marking the anniversary this article looks back to its earliest years and the practicalities faced by its founder, Sir Robert Edwards, in establishing a new journal based on internet publication. The narrative follows the first years of production, with a full-time staff of just two, and how the journal quickly set a forward-looking tone of innovation in reproductive medicine. Despite its name, and despite its originating principle of internet production, since 2010 <em>RBMO</em> has been produced – online and in print – in collaboration with a commercial publisher, which greatly streamlined the management of manuscripts and their rapid publication. While Bob Edwards has remained the inspiration for the journal's progress, each of the editors who succeeded him – Jacques Cohen, Bart Fauser, Juan Garcia-Velasco and Nick Macklon – each gave renewed identity to the title, expanded its reach and nurtured its growth.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104853"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}