Reproductive biomedicine online最新文献

{"title":"Two decades of psychological adjustment of donor-conceived offspring of lesbian parents: examining donor contact and type","authors":"Nicola Carone ,&nbsp;Audrey S. Koh ,&nbsp;Henny M.W. Bos ,&nbsp;Esther D. Rothblum ,&nbsp;Nanette K. Gartrell","doi":"10.1016/j.rbmo.2025.105020","DOIUrl":"10.1016/j.rbmo.2025.105020","url":null,"abstract":"<div><h3>Research question</h3><div>How stable is psychological adjustment in donor-conceived offspring of lesbian parents over 21 years, considering developmental stages, donor type and impact of donor contact?</div></div><div><h3>Design</h3><div>Data were drawn from the US National Longitudinal Lesbian Family Study, a cohort of 70 donor-conceived offspring (71.43% heterosexual, 90.00% White) assessed across four developmental stages – childhood (age 10 years, Wave 4), adolescence (age 17, Wave 5), emerging adulthood (age 25, Wave 6) and established adulthood (ages 30–33, Wave 7). From parental reports, 34.29% had an anonymous donor, 34.29% a known donor and 31.43% an open-identity donor. At Waves 4 and 5, parents completed the Child Behavior Checklist to assess their child’s psychological adjustment, while at Waves 6 and 7 the offspring assessed their psychological adjustment by completing the 120-item Achenbach Adult Self-Report. Growth curve models, stability coefficients and developmental pathway analyses explored trajectories by donor type, while a multivariate analysis examined differences by donor contact.</div></div><div><h3>Results</h3><div>Across donor types, internalizing, externalizing and total problem behaviours showed low relative but high absolute stability, with fluctuations below clinical thresholds. Externalizing and total problem behaviours declined from childhood to adolescence, increased in emerging adulthood and stabilized in established adulthood. Donor type and contact did not significantly impact behavioural outcomes. Over 80% of offspring consistently scored within the normal range across all stages.</div></div><div><h3>Conclusions</h3><div>Findings underscore the psychological resilience of donor-conceived offspring of lesbian parents, irrespective of donor type or contact. This challenges assumptions about donor-related effects on adjustment, highlighting the importance of family relationships. Flexible, family-centred support and consideration of evolving genetic-linking technologies are recommended for policy-makers and practitioners.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 4","pages":"Article 105020"},"PeriodicalIF":3.5,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The importance of considering complexities in the aetiology of adverse outcomes in reproductive medicine","authors":"Grace Dugdale","doi":"10.1016/j.rbmo.2025.105013","DOIUrl":"10.1016/j.rbmo.2025.105013","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 2","pages":"Article 105013"},"PeriodicalIF":3.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical activity, sedentary time and sperm DNA fragmentation index in healthy Japanese men","authors":"Hongxuan Li ,&nbsp;Wen Hao ,&nbsp;Eri Maeda ,&nbsp;Yukiyo Kumazawa ,&nbsp;Kazumasa Takahashi ,&nbsp;Takashi Tanaka ,&nbsp;Isao Yokota ,&nbsp;Yukihiro Terada ,&nbsp;Kyoko Nomura ,&nbsp;Hiroshi Okada ,&nbsp;Akiko Tamakoshi","doi":"10.1016/j.rbmo.2025.105015","DOIUrl":"10.1016/j.rbmo.2025.105015","url":null,"abstract":"<div><h3>Research question</h3><div>Are physical activity and sedentary time associated with the DNA fragmentation index (DFI) score and conventional semen parameters in healthy Japanese men?</div></div><div><h3>Design</h3><div>Baseline data from the Preconception Research and Education Study for Men, an ongoing randomized controlled trial, were used to assess the long-term effects of preconception health education on male fertility. This cross-sectional analysis included 323 men, aged 20–39 years, recruited from September 2021 to December 2022 at workplace health check-up sites in Japan. Demographic, lifestyle and health check-up data were collected, and physical activity and sedentary time were measured using the International Physical Activity Questionnaire-Short Form. Semen quality was assessed using the DFI score and conventional semen analyses. Generalized linear models were used to evaluate the associations between physical activity, sedentary time and semen parameters, adjusting for confounders.</div></div><div><h3>Results</h3><div>Higher total physical activity was associated with a lower DFI score (<em>P</em>-value for trend = 0.002), with the third and fourth total physical activity quartiles showing a 21% (95% CI 5–34%, <em>P</em> = 0.013) and a 29% (95% CI 12–43%, <em>P</em> = 0.002) reduction in DFI score compared with the first quartile reference, respectively. There was a trend for a shorter sedentary time to be associated with a lower DFI score, but this failed to reach significance (<em>P</em>-value for trend = 0.057). The first sedentary time quartile showed a 22% reduction in DFI score compared with the fourth quartile reference (95% CI 3–37%, <em>P</em> = 0.024). There were no significant associations between total physical activity, sedentary time and conventional semen parameters.</div></div><div><h3>Conclusions</h3><div>Higher physical activity and shorter sedentary time are associated with a lower DFI score in Japanese men. However, these associations were not observed with conventional semen parameters.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 4","pages":"Article 105015"},"PeriodicalIF":3.5,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Algorithms to minimize the effect of maternal contamination in non-invasive chromosome screening","authors":"Xiufeng Chang ,&nbsp;Yaxin Yao ,&nbsp;Yangyun Zou ,&nbsp;Yandan lv ,&nbsp;Yu Qiao ,&nbsp;Xin Wang ,&nbsp;Shengnan Zhao ,&nbsp;Jiao Li ,&nbsp;Dunmei Zhao ,&nbsp;Sijia Lu ,&nbsp;Weimin Yang ,&nbsp;Liyi Cai","doi":"10.1016/j.rbmo.2025.104994","DOIUrl":"10.1016/j.rbmo.2025.104994","url":null,"abstract":"<div><h3>Research question</h3><div>Can algorithms be developed to assess maternal contamination in the embryo spent culture medium without parental samples and correct copy number variations (CNV)?</div></div><div><h3>Design</h3><div>An algorithm was developed to confirm the proportion of maternal contamination based on the mosaic ratios of X and Y chromosomes and an algorithm to correct copy number variation (CNV) in embryos with maternal contamination. Performance of algorithms was tested in 317 culture medium samples. A prospective clinical study was then conducted to validate the accuracy.</div></div><div><h3>Results</h3><div>Among 55 male embryos with maternal contamination, the consistency of the corrected CNV results with the whole embryo results was higher than that of the CNV results before correction (69.1% versus 10.9%, <em>P</em> &lt; 0.001). Among the 317 embryo culture medium samples, maternal contamination was identified in 88 (27.8%) samples through the algorithm based on the mosaic ratios of X and Y chromosomes and quantitative parental contamination test. Upon re-assessment using the CNV correction algorithm, two and five of the 19 samples deemed as euploid initially were re-classified as mosaic and aneuploid, respectively; 23 and three of the 36 samples deemed as mosaic initially were re-classified as aneuploid and euploid, respectively. In the clinical study, the live birth rate was 44.7%. Accuracy of the algorithms was assessed in 25 donated embryos and 47 cases with prenatal testing. The overall concordance rate was 95.8% (69/72).</div></div><div><h3>Conclusions</h3><div>The CNV correction algorithm could be used to minimize the effect of maternal contamination in culture medium and enhance the accuracy of non-invasive chromosome screening.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 4","pages":"Article 104994"},"PeriodicalIF":3.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Should a history of pregnancy loss call for an assessment of the ovarian reserve in women 35 years of age or younger?","authors":"Barbara Lawrenz ,&nbsp;Erkan Kalafat ,&nbsp;Jonalyn Edades ,&nbsp;Human Fatemi","doi":"10.1016/j.rbmo.2025.104995","DOIUrl":"10.1016/j.rbmo.2025.104995","url":null,"abstract":"<div><h3>Research question</h3><div>Is a history of pregnancy loss correlated with low age-adapted ovarian reserve in women aged 35 years or younger?</div></div><div><h3>Design</h3><div>This was a retrospective cohort study.</div></div><div><h3>Results</h3><div>A total of 931 women aged 35 years or less with anti-Müllerian-hormone (AMH) values measured at presentation to a fertility centre were included in the analysis. The pregnancy loss rate, modelled as pregnancy loss count per previous pregnancy, increased at both ends of the age-normalized AMH <em>z</em>-score, showing a statistically significant increase (odds ratio 0.49, 95% confidence interval 0.25–0.94; <em>P</em> = 0.032) at the lower end (z-score lower than –1.28, 10th percentile) of AMH values.</div></div><div><h3>Conclusions</h3><div>Women aged 35 years or less are assumed to have a low risk of aneuploidy as a cause of pregnancy loss. While the association between low ovarian reserve and pregnancy loss in this group is still debated, the findings presented describe a significant association between a history of pregnancy loss in women of 35 years or less and an age-adjusted low ovarian reserve. This calls for assessment of the ovarian reserve in women with pregnancy losses, as an early diagnosis of low ovarian reserve may have far-reaching implications for fertility counselling, especially in an era when women often postpone childbearing.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 3","pages":"Article 104995"},"PeriodicalIF":3.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The IVF laboratory: fertile ground for leader development","authors":"Jordon E. Swain ,&nbsp;Russell P. Lemler ,&nbsp;Jason E. Swain","doi":"10.1016/j.rbmo.2025.104991","DOIUrl":"10.1016/j.rbmo.2025.104991","url":null,"abstract":"<div><div>The benefits of effective leadership in healthcare organizations are well documented. With the expansion of fertility care and the importance of the IVF laboratory in the industry, leadership in this unique setting is of paramount importance. However, the traditional pipeline for developing IVF laboratory personnel focuses primarily on technical skills, with little if any formal focus on leader development. Because of this, there are growing calls for key laboratory professionals to receive leadership training. To this end, an evidence-based Leader Development Model is presented and suggestions offered for the advancement of laboratory personnel that will aid in their growth as leaders, allowing them to become more effective in their roles as professionals in the field, yielding benefits for both their teams and the organizations they serve.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 3","pages":"Article 104991"},"PeriodicalIF":3.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic review and evidence-based classification of differentially expressed mRNA in human spermatozoa: insights to improve the diagnosis and prognosis of male infertility","authors":"Ester Anton,&nbsp;Coral Zurera-Egea,&nbsp;Roser Farriol,&nbsp;Zaida Sarrate,&nbsp;Joan Blanco","doi":"10.1016/j.rbmo.2025.104993","DOIUrl":"10.1016/j.rbmo.2025.104993","url":null,"abstract":"<div><div>Since its emergence in the 2000s, the study of sperm RNA has revealed their influence in sperm functions and early embryo development, positioning them as promising markers for the diagnosis of male infertility. This systematic review aimed to evaluate the potential of differentially expressed sperm mRNA associated with infertility to improve diagnostic accuracy, and to identify predictive biomarkers for successful assisted reproductive technology (ART) outcomes. A comprehensive search of the MEDLINE-PubMed database was conducted from inception to 28 April 2023, and a total of 67 eligible articles were selected. Data on 451 genes were extracted and analysed using a custom scoring system, revealing a strong association between altered seminal parameters and the expression of nine mRNA: <em>AKAP4, DDX4, PGK2, PIWIL1, PRM1, PRM2, TNP1, TNP2</em> and <em>PLCZ1</em>. Notably, aberrant expression of <em>PLCZ1, PRM1, PRM2</em> and <em>PIWIL1</em> was closely linked with reduced ART success rates. These findings suggest that these genes constitute a panel of the most prominent biomarkers of broader transcriptomic changes, potentially reflecting disruptions in spermatogenesis. Given the complexity of diagnosing male fertility at a molecular level, integrating sperm transcriptome analysis with conventional semen assessments could enhance fertility predictions, although standardization of methodologies is essential to improve the reliability of the results.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 3","pages":"Article 104993"},"PeriodicalIF":3.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The degree of blastocyst re-expansion immediately after warming is a strong dynamic indicator of embryo quality","authors":"Masato Yoneyama ,&nbsp;Ayumu Ito ,&nbsp;Yukiko Katagiri ,&nbsp;Satoko Oigawa ,&nbsp;Koichi Nagao ,&nbsp;Masahiko Nakata","doi":"10.1016/j.rbmo.2025.104989","DOIUrl":"10.1016/j.rbmo.2025.104989","url":null,"abstract":"<div><h3>Research question</h3><div>Does the degree of blastocyst re-expansion at 9–11 min after warming affect vitrified–warmed blastocyst transfer cycle outcomes, and could it be a quantitative measure suitable for clinical application in assessing embryo quality?</div></div><div><h3>Design</h3><div>This retrospective observational cohort study included 1356 cases of vitrified–warmed blastocyst transfer cycles at the Reproduction Center, Toho University Omori Medical Center between January 2017 and December 2022. The degree of re-expansion was calculated using image-analysis software. The degree of blastocyst re-expansion at about 9–11 min after warming and its association with cycles outcomes were evaluated. Clinical pregnancy was the primary outcome.</div></div><div><h3>Results</h3><div>Blastocysts were grouped at 9–11 min after warming based on a calculated cut-off value for the degree of re-expansion (90.2%): the ‘re-expansion group’ (≥90.2% without hatching), the ‘shrinkage group’ (&lt;90.2%) and the ‘hatching group’. Implantation, clinical pregnancy, ongoing pregnancy and live birth rates were significantly higher in the re-expansion versus shrinkage group (<em>P</em> &lt; 0.001) and were comparable with the hatching group. Logistic regression analysis revealed age, the degree of blastocyst re-expansion 90.2% or higher, inner cell mass quality and trophectoderm quality as independent predictors of clinical pregnancy, whereas additional variables showed no significant adjusted odds ratios.</div></div><div><h3>Conclusions</h3><div>The degree of blastocyst re-expansion at 9–11 min after warming is a strong dynamic indicator of embryo quality, offering a novel metric distinct from traditional morphological assessments. This study highlights the importance of rapid re-expansion as a predictor of clinical pregnancy and underscores its potential utility in enhancing the selection process for embryo transfer.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 5","pages":"Article 104989"},"PeriodicalIF":3.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic signature of menstrual blood mesenchymal stromal cells and their extracellular vesicles in women with unexplained infertility","authors":"Raminta Vaiciuleviciute ,&nbsp;Kieran Brennan ,&nbsp;Ilona Uzieliene ,&nbsp;Jolita Pachaleva ,&nbsp;Zaneta Kasilovskiene ,&nbsp;Lina Piesiniene ,&nbsp;Daiva Bironaite ,&nbsp;Margaret M. Mc Gee ,&nbsp;Eiva Bernotiene","doi":"10.1016/j.rbmo.2025.104980","DOIUrl":"10.1016/j.rbmo.2025.104980","url":null,"abstract":"<div><h3>Research question</h3><div>Can the proteome of menstrual blood mesenchymal stromal cells (MenSC), their extracellular vesicles or extracellular-vesicle-depleted supernatant be employed for personalized identification of altered cellular processes in women with unexplained infertility (uIF), enabling their stratification into aetiopathogenetic endotypes?</div></div><div><h3>Design</h3><div>For MenSC isolation, menstrual blood was collected from 12 fertile healthy volunteers (control group) and eight patients with uIF. MenSC extracellular vesicles were isolated using iodixanol density gradient centrifugation, quantified by flow cytometry and characterized in accordance with the Minimal Information for Studies of Extracellular Vesicles (MISEV) guidelines by transmission electron microscopy, flow cytometry and western blot analysis. Proteomic analysis was undertaken using mass spectrometry and bioinformatic tools.</div></div><div><h3>Results</h3><div>Mesenchymal marker expression and phenotype of MenSC isolated from the menstrual blood of fertile females and females with uIF did not differ between the groups. MenSC-derived extracellular vesicles were isolated successfully from the conditioned media, and characterized in accordance with the MISEV guidelines. The comprehensive proteomic analysis of MenSC, MenSC extracellular vesicles and extracellular-vesicle-depleted MenSC supernatant showed significant changes in the proteome in all three sample types of uIF, mainly implying compromised cell adhesion, immune response, fibrosis and altered metabolism. These findings were validated by western blot analysis.</div></div><div><h3>Conclusions</h3><div>Proteome changes in cell adhesion, immune and inflammatory processes, and protein metabolism were observed in menstrual blood samples of the uIF group. The proteins responsible for these processes may be used as biomarkers to stratify patients into distinct molecular endotypes characterized by altered adhesion, immune response, fibrosis and metabolism, which may lead to the development of personalized treatment approaches for women with uIF.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 4","pages":"Article 104980"},"PeriodicalIF":3.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144887230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenomyosis: the missed disease","authors":"Pietro Santulli ,&nbsp;Silvia Vannuccini ,&nbsp;Mathilde Bourdon ,&nbsp;Charles Chapron ,&nbsp;Felice Petraglia","doi":"10.1016/j.rbmo.2025.104837","DOIUrl":"10.1016/j.rbmo.2025.104837","url":null,"abstract":"<div><div>Adenomyosis, a menstruation-related uterine disorder, refers to the presence of endometrial stroma and glands within the myometrium and is typically observed in reproductive-age women. The pathogenesis explaining the migration, persistence, proliferation and differentiation of ectopic endometrial cells includes a genetic and epigenetic background, an oestrogen/progesterone receptor imbalance and an inflammatory reaction driven by local immune dysfunction, along with fibrosis and neuroangiogenesis within the myometrium. In the past, it was thought that adenomyosis almost exclusively affected multiparous women after 40 years of age and the diagnosis was generally confirmed upon hysterectomy. Nowadays, using imaging techniques such as transvaginal ultrasonography and magnetic resonance imaging, adenomyosis is increasingly identified in young women with dysmenorrhoea, dyspareunia, abnormal uterine bleeding and heavy menstrual bleeding, and also in infertile patients. Furthermore, adenomyosis often coexists with other gynaecological conditions, such as endometriosis and uterine fibroids. Despite the improvement of non-invasive diagnostic tools, the awareness of the condition is still poor and the diagnosis is often missed, due also to a heterogeneity in clinical presentation and imaging criteria. In addition, medical and surgical management do not follow shared recommendations, even though adenomyosis requires a lifelong management plan, including pain and bleeding control, fertility preservation and pregnancy complications.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 4","pages":"Article 104837"},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}