Reproductive biomedicine online最新文献

{"title":"Investigating phototoxicity of optical coherence tomography imaging in porcine and human spermatozoa","authors":"Marie Claire Aquilina ,&nbsp;Julien Camard ,&nbsp;Wisdom Igiogbe ,&nbsp;Taylor Sanderson ,&nbsp;Lucy Abbott ,&nbsp;Darren K. Griffin ,&nbsp;Adrian Podoleanu ,&nbsp;Peter Ellis ,&nbsp;Giuseppe Silvestri ,&nbsp;Manuel J. Marques","doi":"10.1016/j.rbmo.2025.105175","DOIUrl":"10.1016/j.rbmo.2025.105175","url":null,"abstract":"<div><h3>Research question</h3><div>Does exposure to near-infra-red optical coherence tomography (OCT) laser radiation induce phototoxic effects in porcine and human spermatozoa?</div></div><div><h3>Design</h3><div>Computer-assisted sperm analysis (CASA) was used to determine whether OCT laser illumination at or above levels typically used for imaging alters sperm motility. Flow cytometry was used to determine the impact of irradiation on sperm acrosome reaction status, DNA fragmentation, and membrane integrity. Additionally, in-vitro time-lapse OCT imaging of a porcine cumulus–oocyte complex with irradiated spermatozoa was performed to determine whether irradiated spermatozoa interact with and penetrate the cumulus oophorus. Finally, human spermatozoa were irradiated and analysed using CASA and flow cytometric techniques.</div></div><div><h3>Results</h3><div>All irradiated samples showed no significant difference in sperm DNA damage or CASA sperm motility parameters, including average path velocity, straight line velocity or curvilinear velocity, compared with their matched manipulation control, suggesting that sample irradiation did not compromise sperm viability, even when using an optical power of more than one order of magnitude greater than that typically required to image embryos. Proof-of-concept OCT imaging suggested that motility of irradiated spermatozoa during cumulus interaction was not affected by radiation.</div></div><div><h3>Conclusions</h3><div>No significant effect on the kinetics of boar and human spermatozoa was observed following near-infra-red OCT laser radiation. Future work will investigate the fertilization process and embryo development following near-infra-red OCT laser radiation.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105175"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145596979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of constant (5%) versus gradient (8%–2%) oxygen concentration on sibling human blastocyst development","authors":"Peter Slatinšek ,&nbsp;Milan Reljič ,&nbsp;Borut Kovačič","doi":"10.1016/j.rbmo.2025.105207","DOIUrl":"10.1016/j.rbmo.2025.105207","url":null,"abstract":"<div><h3>Research question</h3><div>Does a gradient of oxygen concentration, decreasing from 8% to 2% during prolonged embryo culture, yield a higher percentage of morphologically optimal blastocysts compared with a static 5% oxygen atmosphere?</div></div><div><h3>Design</h3><div>This prospective sibling-split study included 658 cumulus-oocyte complexes from 44 intracytoplasmic sperm injection (ICSI) cycles (January 2022 to January 2023), alternately assigned to a control group (static 5% oxygen) or an intervention group (stepwise oxygen reduction: 8% on days 0-3, 5% on day 3, and 2% until days 5 or 6).</div></div><div><h3>Results</h3><div>Early embryo morphology was similar between groups. By day 5, following exposure to 2% oxygen from day 3, the control group produced a significantly higher proportion of clinically used blastocysts (47.3%, 122/258 vs. 36.9%, 97/263; <em>P</em> = 0.0161). Rates of morphologically optimal day-5 blastocysts were comparable (14.7%, 38/258 vs. 10.3%, 27/263; P = 0.2373). Time-lapse analysis showed delayed initiation of blastulation (P = 0.0024), blastocyst formation (<em>P</em> &lt; 0.001), and expansion (<em>P</em> &lt; 0.001) in the intervention group. Because of this delay, embryologists were more likely to select blastocysts for fresh transfer from the control group in a blinded procedure (<em>P</em> = 0.0088). Morphometric analysis showed significantly larger blastocyst cross-sectional area in the control group (28,198 ± 4139 vs. 25,994 ± 4657 µm²; <em>P</em> = 0.0014), consistent with more advanced expansion under constant 5% oxygen.</div></div><div><h3>Conclusions</h3><div>Gradually lowering oxygen from 8% to 2% during the second half of embryo culture resulted in delayed blastulation and fewer clinically used blastocysts when compared with culture under static 5% oxygen.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105207"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double-stranded sperm DNA fragmentation assessed using comet assay is associated with recurrent pregnancy loss","authors":"Andrew Drakeley ,&nbsp;Rebecca L. Lunt ,&nbsp;Emily Newton ,&nbsp;Rachel C. Gregoire ,&nbsp;Linda Watkins ,&nbsp;Katerina Bambang ,&nbsp;Ann-Maria Ellard ,&nbsp;Craig J. Ledgerwood ,&nbsp;Lewis Rae ,&nbsp;Alastair Sloan ,&nbsp;Martin Lawlor ,&nbsp;Lynsey Poots ,&nbsp;Luca Moore ,&nbsp;Elizabeth Bailie ,&nbsp;Sara H. McDowell ,&nbsp;Jordi Ribas-Maynou ,&nbsp;Tara Moore","doi":"10.1016/j.rbmo.2025.105184","DOIUrl":"10.1016/j.rbmo.2025.105184","url":null,"abstract":"<div><h3>Research question</h3><div>Do men attending a recurrent miscarriage clinic have high double-stranded sperm DNA damage compared with a sperm donor population?</div></div><div><h3>Design</h3><div>This prospective cohort study included 100 men attending a recurrent miscarriage clinic, and 81 sperm donors from a European sperm bank who had proven fertility. All semen samples were evaluated using the Examen Lab alkaline (Exact) comet assay, which identifies the global (single and double strand) DNA damage, and the neutral (Extend) comet assay, measuring only double-stranded sperm DNA fragmentation (dsSDF).</div></div><div><h3>Results</h3><div>Semen analysis showed that 66 male partners of women with a history of recurrent miscarriage were classified as being within normal parameters. Of these, however, 66 men (61%) had raised global SDF, and 52% had raised dsSDF. When evaluating each test separately, the Exact comet (global SDF) assay presented an area under the curve (AUC) of 0.690 (95% CI 0.623–0.756), with the neutral Extend comet (dsSDF only) assay having an AUC of 0.876 (95% CI 0.834–0.914), and the incidence of damage showed an improved AUC of 0.909 (95% CI 0.874–0.940).</div></div><div><h3>Conclusions</h3><div>This study of male factor SDF in a large cohort of men attending a recurrent miscarriage clinic, where they are rarely the focus of clinical investigation, shows a strong association with dsSDF and male factor-driven miscarriage contribution, highlighting the importance of male investigation in couples experiencing recurrent pregnancy loss.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105184"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145725627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fast and furious: live birth outcomes in 1266 infants born after one-step warming of vitrified human blastocysts","authors":"Juergen Liebermann,&nbsp;Rebecca Brohammer,&nbsp;Yuri Wagner,&nbsp;Allison Parus,&nbsp;Cynthia Macias,&nbsp;Nivek Suda,&nbsp;Alexis Vanderpool,&nbsp;Gabija Petrus,&nbsp;Olivia Sarris,&nbsp;Kelly Even,&nbsp;Ru Smith,&nbsp;Shelby Sutherland,&nbsp;Sue Jasulaitis,&nbsp;Jennifer Hirshfeld-Cytron,&nbsp;Christopher Sipe,&nbsp;Meike Uhler","doi":"10.1016/j.rbmo.2025.105238","DOIUrl":"10.1016/j.rbmo.2025.105238","url":null,"abstract":"<div><h3>Research question</h3><div>Does one-step rehydration in the warming protocol for human blastocysts result in healthy live births?</div></div><div><h3>Design</h3><div>A total of 2411 frozen embryo transfers using a short one-step rehydration protocol and 2606 frozen embryo transfers using the multi-step protocol were available for analysis. One-step warming was performed at 37°C in 1M sucrose for 1 min. This study examined the gestational age at delivery and birth weight of male and female infants for both protocols.</div></div><div><h3>Results</h3><div>In the one-step warming protocol, 1266 infants were born. The overall live birth rate was 51.3%, average gestational age was 37.6 weeks, and average birth weight was 3267 g. The sex distribution was 49% male versus 51% female. Male infants were, on average, 70 g heavier than female infants (3305 g versus 3235 g, respectively). In the multi-step warming protocol, 1323 infants were born. The average gestational age was 37.6 weeks, and average birth weight was 3252 g. The sex distribution was 52% male versus 48% female. Male infants were, on average, 139 g heavier than female infants (3318 g vs 3179 g, respectively). There were no significant differences between the two warming groups in terms of live birth and miscarriage rates, gestational age and birth weight.</div></div><div><h3>Conclusions</h3><div>Short one-step rehydration during warming of human blastocysts for 1 min in 1M sucrose led to healthy infants born at term with corresponding birth weight. This one-step rehydration protocol has been shown to be safe and effective, and it has now been validated with 1266 live births.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105238"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145757525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-life experience with transvaginal radiofrequency ablation in infertile patients with types 2 and 3 fibroids","authors":"María Cerrillo ,&nbsp;Javier Martínez-Salazar ,&nbsp;Cristina Fernández ,&nbsp;Pedro Royo ,&nbsp;Alberto Vázquez ,&nbsp;Elkin Muñoz ,&nbsp;Víctor H. Gómez ,&nbsp;José Puente ,&nbsp;Antonio Requena ,&nbsp;Juan A. García-Velasco","doi":"10.1016/j.rbmo.2025.105104","DOIUrl":"10.1016/j.rbmo.2025.105104","url":null,"abstract":"<div><h3>Research question</h3><div>Transvaginal radiofrequency ablation (TRFA) may offer a safe and effective alternative to surgery for infertile women with type 2 or 3 fibroids under 4 cm diameter that compromise the uterine cavity.</div></div><div><h3>Methods</h3><div>In this study, 36 patients underwent ultrasound-guided TRFA between 2020 and 2023. Main outcomes were fibroid volume reduction at 1, 3, and 6 months, and post-procedure reproductive results.</div></div><div><h3>Results</h3><div>Mean baseline fibroid volume was 19.29 cm³, decreasing to 9.6 cm³ at 1 month, 4.39 cm³ at 3 months, and 4.07 cm³ at 6 months, reflecting &gt;75% reduction. Mild pain and minimal bleeding occurred in 22% of patients; hysteroscopic removal of protruding fibroids was required in 22%. Among the 25 women undergoing subsequent ART, pregnancy and miscarriage rates per treated patient were 68% and 16%, respectively; live births occurred in 52%, with most deliveries at full-term. Caesarean delivery rate was 69.2%.</div></div><div><h3>Conclusions</h3><div>TRFA appears to be a safe and effective alternative to surgery for infertile women with type 2 or 3 fibroids under 4 cm in diameter that compromise the uterine cavity. The procedure does not seem to affect reproductive outcomes, and this study is the first to assess the effect of TRFA on ART.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105104"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assisted reproductive technology in Latin America: the Latin American Registry, 2022","authors":"Fernando Zegers-Hochschild ,&nbsp;Javier A Crosby ,&nbsp;Carolina Musri ,&nbsp;Fanny Petermann-Rocha ,&nbsp;Gustavo Martinez ,&nbsp;Hitomi Nakagawa ,&nbsp;Carlos Morente ,&nbsp;Armando Roque ,&nbsp;Ana Palma-Govea ,&nbsp;Latin American Network of Assisted Reproduction","doi":"10.1016/j.rbmo.2025.105216","DOIUrl":"10.1016/j.rbmo.2025.105216","url":null,"abstract":"<div><h3>Research question</h3><div>What are the trends and effects of assisted reproductive technology (ART) interventions on the effectiveness and safety of ART carried out in Latin America during 2022.</div></div><div><h3>Design</h3><div>Retrospective collection of cycle-based multinational data obtained from ART procedures carried out by 204 accredited institutions in 16 countries.</div></div><div><h3>Results</h3><div>In total 123,265 initiated cycles resulted in 19,663 deliveries and 22,203 births. Use of ART varied greatly, from 643.3 cycles/million inhabitants in Uruguay to 28.8 in Guatemala. In autologous cycles, the proportion of women aged ≥40 years represents 35.1% of cycles, whereas women &lt;34 years represents only 18.8%. The proportion of single embryo transfers (SET) increased from 42.4% in 2021 to 47.3% in 2022. Out of 22,203 babies born, 77.4% were singletons, 21.7% twins and 0.9% triplets or more. Intracytoplasmic sperm injection represented 85.4% of fertilization techniques, and blastocyst transfer increased from 79.3% in 2021 to 85.3% in 2022. Delivery rate after fresh blastocyst elective single embryo transfer (32.6%) was significantly higher than after the transfer of one frozen embryo transfer (FET) from freeze-all cycle (25.2%) (<em>P</em> = 0.0001). The number of aspirations leading to preimplantation genetic testing increased 2.6 times in 6 years, and significantly increased delivery rates/transfer (<em>P</em> ≤ 0.008) and reduced miscarriage at all ages (<em>P</em> ≤ 0.004) in autologous cycles, but not in oocyte donation cycles. Delivery rates after fresh transfer of embryos from vitrified–warmed donated oocytes, generated similar outcome to FET from fresh oocyte donation cycles (<em>P</em> = 0.5621). Perinatal mortality increased from 7.5‰ in singletons to 22.8‰ in twins.</div></div><div><h3>Conclusion</h3><div>Systematic collection of cycle-based multinational data contributes to cooperative sustained development and helps implement evidence-based reproductive decisions.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105216"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-step warming technique for vitrified human blastocysts using commercially available thawing solutions","authors":"Rena Kato ,&nbsp;Fumiaki Itoi ,&nbsp;Aiko Tajima,&nbsp;Nozomi Oono,&nbsp;Kanae Mori,&nbsp;Sumika Watanabe,&nbsp;Rio Tanaka,&nbsp;Mao Akita,&nbsp;Hinano Touyama,&nbsp;Mao Ibata,&nbsp;Saki Nakamura,&nbsp;Mika Kamata,&nbsp;Yukio Hattori","doi":"10.1016/j.rbmo.2025.105168","DOIUrl":"10.1016/j.rbmo.2025.105168","url":null,"abstract":"<div><h3>Research question</h3><div>What are the re-expansion rates and clinical outcomes obtained when employing a one-step warming technique using three commercially available thawing solutions on vitrified blastocysts, and how do they compare with the standard multi-step warming technique?</div></div><div><h3>Design</h3><div>This retrospective cohort study involved 1173 frozen embryo transfers (FET) in women aged &lt;42 years between December 2022 and December 2024. The blastocysts were warmed following multi-step warming guidelines (with Reprolife solutions) or using one-step warming with 1 min of immersion in a thawing solution. Warmed blastocysts were cultured in a recovery medium for 2–3 h, and then transferred to the embryo transfer medium. Embryo transfer was performed after another 2 h. Survival, re-expansion and clinical outcomes were compared between 573 FET using one-step warming and 600 FET using multi-step warming, as well as between one-step warming using three different brands of thawing solution: Irvine (319 FET), Kitazato (128 FET) and Reprolife (126 FET).</div></div><div><h3>Results</h3><div>The re-expansion rate of blastocysts after 4–5 h was significantly higher with one-step warming (92.4%) compared with multi-step warming (86.8%) (<em>P</em> = 0.0009). The clinical pregnancy rate was also significantly higher with one-step warming (69.3%) compared with multi-step warming (61.2%) (<em>P</em> = 0.0042), but the ongoing pregnancy rate did not differ significantly between one-step warming (53.9%) and multi-step warming (49.2%). No significant differences in re-expansion or clinical outcomes were detected between the three different brands of thawing solution used for one-step warming.</div></div><div><h3>Conclusions</h3><div>One-step warming results in better re-expansion and clinical outcomes than multi-step warming, and one-step warming is equally effective with the three commercially available brands of thawing solution tested.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105168"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145605502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does total motile sperm count predict embryo quality and ICSI success in young normal-weight women? A retrospective multicentre study","authors":"Andrea Crafa ,&nbsp;Rossella Cannarella ,&nbsp;Ana Navarro-Gomezlechon ,&nbsp;Rosa M Pacheco-Rendón ,&nbsp;Rosita A Condorelli ,&nbsp;Sandro La Vignera ,&nbsp;Aldo E Calogero ,&nbsp;Nicolás Garrido","doi":"10.1016/j.rbmo.2025.105209","DOIUrl":"10.1016/j.rbmo.2025.105209","url":null,"abstract":"<div><h3>Research question</h3><div>Can the fresh and post-selection total motile sperm count (TMSC) predict intracytoplasmic sperm injection (ICSI) outcomes?</div></div><div><h3>Design</h3><div>This multicentre retrospective cohort study involved couples with infertility (&gt;12 months) undergoing ICSI. Participants were divided into five groups by baseline TMSC: group I, less than 1 million; group II, 1 to less than 5 million; group III, 5 to less than 10 million; group IV, 10–20 million; and group V, over 20 million. The primary outcome was the cumulative live birth rate, with secondary outcomes of oocyte fertilization rate, embryo number/quality, biochemical and clinical pregnancy rates and miscarriage rate.</div></div><div><h3>Results</h3><div>In total 5813 couples met the inclusion criteria. After adjustment for confounding factors, the TMSC of fresh samples was positively correlated with the oocyte fertilization rate, with higher values observed in all groups compared with group I (<em>P</em> &lt; 0.001). Moreover, TMSC appears to influence the cumulative live birth rate for oocytes used (hazard ratio [HR] 1.25, 95% CI 1.05-1.48, adjusted <em>P</em> = 0.0116), while no difference was observed when the number of embryos transferred and transfers were considered. Finally, no difference was found in embryo quality or other reproductive outcomes. For post-selection TMSC, none of the groups had a higher fertilization rate than group I after adjusting for confounding factors.</div></div><div><h3>Conclusions</h3><div>This study suggests that changes in TMSC alone can predict the oocyte fertilization rate and slightly influence the cumulative live birth rate, particularly when considering the total number of inseminated oocytes. These underscore the critical role of sperm quality in the success of ICSI. However, other female and/or male parameters may also contribute to the outcomes.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105209"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Concern: Adding phytoestrogens to clomiphene induction in unexplained infertility patients – a randomized trial. [Reproductive BioMedicine Online, 2008, Vol 16. No 4, 580-588]","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"53 1","pages":"Article 105771"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148184442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence-assisted selective modified natural frozen embryo transfer","authors":"Michal Youngster ,&nbsp;Nevo Itzhak ,&nbsp;Eden Moran ,&nbsp;Shachar Reuvenny ,&nbsp;Almog Luz ,&nbsp;Rohi Hourvitz ,&nbsp;Micha Baum ,&nbsp;Ettie Maman ,&nbsp;Ariel Hourvitz","doi":"10.1016/j.rbmo.2025.105084","DOIUrl":"10.1016/j.rbmo.2025.105084","url":null,"abstract":"<div><h3>Research question</h3><div>Can an artificial intelligence algorithm assist in selectively triggering natural-cycle frozen embryo transfer (NC-FET) to avoid transfers on undesired days?</div></div><div><h3>Design</h3><div>This was a retrospective cohort study including patients undergoing 4029 FET cycles (2018–2024). The proposed algorithm used a previously developed ovulation prediction model that can predict ovulation up to 6 days in advance using LH, oestradiol, progesterone and follicle size. The algorithm predicted the probability of natural ovulation occurring in the days following each clinic visit and suggested triggering only in cases where a transfer was likely to occur on days the clinic would be closed.</div></div><div><h3>Results</h3><div>The results of the current analysis represent a selected scenario for a clinic that is closed on Sundays and has minimal staffing on Saturdays. Other clinic schedules can be configured. In around 40% of cycles, the algorithm estimated ovulation occurring on a day leading to a non-working day transfer so triggering was suggested. These cases resulted in a shift of ovulation by 2 days or fewer in 89% of cases, and 4 or more days in 2% of cases. In the remaining approximately 60% of cases, the algorithm did not intervene in natural ovulation, identifying ovulation correctly in 96% of cases.</div></div><div><h3>Conclusions</h3><div>The use of a selective modified NC-FET algorithm can minimize the number of transfers performed on non-working days, while keeping the majority of cycles completely natural, enabling flexibility when needed, and maintaining the advantages of natural cycles when possible. Implementation of this algorithm may provide a solution for clinics looking to increase their NC-FET practice without compromising convenience.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105084"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}