Reproductive biomedicine online最新文献

{"title":"Fertility preservation in endometriosis: evaluating surgical risks and emerging preservation approaches","authors":"Neda Farzizadeh ,&nbsp;Zahra Najmi ,&nbsp;Alan J. Rosenbaum ,&nbsp;Arezoo Khosravi ,&nbsp;Amirali Hariri ,&nbsp;Mona Aminbeidokhti ,&nbsp;Ali Zarrabi","doi":"10.1016/j.rbmo.2025.105212","DOIUrl":"10.1016/j.rbmo.2025.105212","url":null,"abstract":"<div><div>Endometriosis poses significant challenges for women of reproductive age, particularly due to its impact on ovarian reserve and fertility. In addition to endometriosis-associated infertility contributing to diminished ovarian reserve by inflammation and oxidative stress, surgical interventions, often required to manage endometriosis, can exacerbate ovarian damage, further complicating fertility preservation. This narrative review critically examines the interplay between endometriosis treatment and fertility preservation strategies, offering insights into current surgical risks and emerging approaches to mitigate their impact on reproductive potential. Furthermore, it explores traditional fertility preservation methods, including oocyte, embryo and ovarian tissue cryopreservation, alongside advances in vitrification techniques that enhance success rates. Innovative minimally invasive surgical techniques, such as carbon dioxide laser vaporization, plasma energy ablation, haemostatic sealants, and robotic-assisted laparoscopy, are evaluated for their ability to preserve ovarian tissue. Moreover, emerging trends, including the use of artificial intelligence for personalized treatment planning and bioengineering innovations, such as three-dimensional-printed ovarian scaffolds, are discussed as transformative solutions for restoring fertility. A multi-disciplinary, patient-centred approach is emphasized, integrating expertise from gynaecology, reproductive endocrinology, and bioengineering to optimize fertility preservation outcomes. By bridging technological advances and clinical practice, this review aims to provide a framework for preserving fertility while addressing the complex challenges of endometriosis.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105212"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145605317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preimplantation genetic testing for aneuploidy ‘rescues’ poor-quality blastocysts and increases embryo availability for transfer: a 9-year single centre analysis","authors":"Balsam Al Hashimi ,&nbsp;Katie E Harvey ,&nbsp;Simon C Harvey ,&nbsp;Elena Linara-Demakakou ,&nbsp;Darren K Griffin ,&nbsp;Kamal Ahuja ,&nbsp;Nick Macklon","doi":"10.1016/j.rbmo.2025.105208","DOIUrl":"10.1016/j.rbmo.2025.105208","url":null,"abstract":"<div><h3>Research question</h3><div>Does preimplantation genetic testing for aneuploidy (PGT-A) and the transfer of euploid poor-quality blastocysts (PQB) reduce the number of transfers needed to achieve live births compared with the transfer of their untested counterparts?</div></div><div><h3>Design</h3><div>Single-centre retrospective cohort study conducted between 2015 and 2024 (PGT-A blastocysts: <em>n</em> = 7332 from 2258 cycles; <em>n</em> = 1344). Transfer outcomes were analysed for a subset of 74 cycles involving tested PQB and compared with 192 cycles involving untested PQB during the same period.</div></div><div><h3>Results</h3><div>High-quality blastocysts (AA, AB, BA and BB) were most likely to be euploid (<em>P</em> &lt; 2.2e-16), whereas PQB (CC, DC, CD and DD) were more likely to be aneuploid (<em>P</em> &lt; 2.2e-16). Embryos that reached the blastocyst stage by day 5 had a higher likelihood of being euploid. Among transferred PQB, PGT-A did not significantly affect the pregnancy rate (33.3% versus 23.4%); however, the miscarriage rate was significantly lower in the PGT-A-tested group (13.6% versus 51.2%, <em>P</em> = 0.003). The number of live births was higher in the PGT-A group (26.4 % versus 11.1%, <em>P</em> = 0.004) and with the transfer of day-5 frozen blastocysts. Live births were observed from blastocysts with the poorest expansion and morphology scores. No significant differences were observed in gestational age or birth weight between the PGT-A and untested groups.</div></div><div><h3>Conclusions</h3><div>A clinically relevant proportion of PQB are euploid, PQB can result in live births and euploid transfer is associated with lower miscarriage rates. In combination, this suggests that PQB should not be routinely discarded, particularly if they are prior-tested using PGT-A.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105208"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145820490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Matter - Continued TOC","authors":"","doi":"10.1016/S1472-6483(25)00655-8","DOIUrl":"10.1016/S1472-6483(25)00655-8","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105448"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inside Front Cover - Affiliations and First page of TOC","authors":"","doi":"10.1016/S1472-6483(25)00654-6","DOIUrl":"10.1016/S1472-6483(25)00654-6","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105447"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Editorial Board","authors":"","doi":"10.1016/S1472-6483(25)00665-0","DOIUrl":"10.1016/S1472-6483(25)00665-0","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 1","pages":"Article 105458"},"PeriodicalIF":3.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NEAT1 inhibits granulosa cell apoptosis and promotes cell growth and migration through the miR-130a-3p/BMP6 axis","authors":"Wen Chen ,&nbsp;Li Dong ,&nbsp;Qihui Liang ,&nbsp;Zhicheng Jia ,&nbsp;Qiaosong Han ,&nbsp;Qiyu Yang ,&nbsp;Yuxuan Zhao ,&nbsp;Haicui Wu","doi":"10.1016/j.rbmo.2025.104870","DOIUrl":"10.1016/j.rbmo.2025.104870","url":null,"abstract":"<div><h3>Research question</h3><div>What are the molecular mechanisms of action of nuclear paraspeckle assembly transcript 1 <em>(NEAT1)</em>, miR-130a-3p and bone morphogenetic protein 6 <em>(BMP6)</em> in regulating ovarian granulosa cell (OGC) apoptosis and dysfunction in women with diminished ovarian reserve (DOR)?</div></div><div><h3>Design</h3><div>Follicular fluid was collected from 20 women undergoing oocyte retrieval: 10 with DOR and 10 with normal ovarian reserve (NOR). OGC were isolated and assayed for expression of <em>NEAT1</em>, miR-130a-3p and <em>BMP6</em>. Granulosa-like tumour cell line (KGN) cells were used to investigate <em>NEAT1</em> function by gene silencing and overexpression.</div></div><div><h3>Results</h3><div><em>NEAT1</em> and <em>BMP6</em> expression were significantly lower in DOR-derived OGC compared with NOR-derived OGC (<em>P</em> &lt; 0.001), while miR-130a-3p expression was elevated (<em>P</em> &lt; 0.001). <em>NEAT1</em> expression was positively correlated with embryo number (<em>r</em> = 0.505, <em>P</em> = 0.023), number of high-quality embryos (<em>r</em> = 0.757, <em>P</em> &lt; 0.001), two-pronuclei fertilization rate (<em>r</em> = 0.477, <em>P</em> = 0.033) and oocyte yield (<em>r</em> = 0.511, <em>P</em> = 0.021). Dual-Luciferase Reporter assays demonstrated that miR-130a-3p binds directly to <em>NEAT1</em> and <em>BMP6</em> 3′UTR (<em>P</em> &lt; 0.001). Functionally, <em>NEAT1</em> overexpression significantly promoted the proliferation and migration of KGN cells, while suppressing apoptosis (<em>P</em> &lt; 0.001); these effects were partially attenuated by miR-130a-3p mimic transfection. Conversely, inhibition of miR-130a-3p significantly reduced apoptosis, and enhanced proliferation and migration of KGN cells (<em>P</em> &lt; 0.001); these effects were partially reversed upon <em>BMP6</em> silencing.</div></div><div><h3>Conclusions</h3><div><em>NEAT1</em> inhibits apoptosis and promotes proliferation and migration of OGC through the miR-130a-3p/<em>BMP6</em> axis, which may improve oocyte quality in patients with DOR. As such, <em>NEAT1</em> may be a potential prognostic biomarker and therapeutic target for DOR.</div></div><div><h3>Clinical trial registration number</h3><div>ChiCTR2400079975</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 6","pages":"Article 104870"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145418377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative proteomic analysis of receptive and non-receptive endometria-derived uterine fluid suggests a panel for endometrial receptivity assessment","authors":"Farnoosh Saraee ,&nbsp;Faezeh Shekari ,&nbsp;Ashraf Moini ,&nbsp;Mehdi Mirzaei ,&nbsp;Pooneh Ghaznavi ,&nbsp;Shabnam Khodabakhshi ,&nbsp;Anna Meyfour ,&nbsp;Sara Fayazzadeh ,&nbsp;Azadeh Ghaheri ,&nbsp;Najmeh Salehi ,&nbsp;Mohammad Reza Ghaffari ,&nbsp;Ghasem Hosseini Salekdeh ,&nbsp;Poopak Eftekhari Yazdi ,&nbsp;Mehdi Totonchi","doi":"10.1016/j.rbmo.2025.105083","DOIUrl":"10.1016/j.rbmo.2025.105083","url":null,"abstract":"<div><h3>Research question</h3><div>Can proteomic analysis of uterine fluid identify a panel of proteins that could potentially improve targeted embryo transfer strategies in IVF?</div></div><div><h3>Design</h3><div>Uterine fluid samples were collected from 18 patients who had undergone in vitro fertilization (IVF) with frozen embryo transfer 2 days before embryo transfer (11 patients in the main cohort and 7 patients in the independent cohort). Seven weeks later, patients were categorized into receptive and non-receptive groups based on ultrasonography results confirming clinical pregnancy. The proteins in these samples were analysed using tandem mass tag labelling and mass spectrometry. Differentially abundant proteins between the two groups were identified, and the candidate protein panel was selected based on the variable of importance in projection score and protein database analysis. The accuracy of the candidate panel was assessed using logistic regression, and findings were confirmed through western blot analysis.</div></div><div><h3>Results</h3><div>Bioinformatics analysis revealed that proteins related to protein synthesis, cell adhesion, keratinization, peroxisome proliferator-activated receptors, arachidonic acid metabolism and vascular endothelial growth factor were enriched in the receptive group. Biological processes, such as receptor internalization, negative regulation of cell junctions, the innate immune response, inflammatory response, and actin cytoskeleton organization were enriched in the non-receptive group. The key proteins, including desmoplakin, keratin type II cytoskeletal 1, the neuroblast differentiation-associated proteins AHNAK, moesin, and fibulin-1 were confirmed as potential biomarkers for assessing endometrial receptivity via western blot analysis.</div></div><div><h3>Conclusion</h3><div>The candidate panel may provide an approach for evaluating endometrial receptivity in the embryo transfer cycle, potentially improving personalized embryo transfer timing in clinical settings.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 6","pages":"Article 105083"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145445683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Matter - Continued TOC","authors":"","doi":"10.1016/S1472-6483(25)00590-5","DOIUrl":"10.1016/S1472-6483(25)00590-5","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 6","pages":"Article 105383"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145614789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to: Representation of study in recent RBMO Commentary","authors":"Juan A. Garcia-Velasco,&nbsp;Michael Alper,&nbsp;Antonio Capalbo,&nbsp;Robert Casper,&nbsp;Human Fatemi,&nbsp;Thomas Molinaro,&nbsp;Filipo Ubaldi,&nbsp;Antonio Pellicer","doi":"10.1016/j.rbmo.2025.105338","DOIUrl":"10.1016/j.rbmo.2025.105338","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 6","pages":"Article 105338"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Editorial Board","authors":"","doi":"10.1016/S1472-6483(25)00599-1","DOIUrl":"10.1016/S1472-6483(25)00599-1","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"51 6","pages":"Article 105392"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145614791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}