Reproductive biomedicine online最新文献

{"title":"Double vitrification and warming of blastocysts does not affect pregnancy, miscarriage or live birth rates","authors":"","doi":"10.1016/j.rbmo.2024.104103","DOIUrl":"10.1016/j.rbmo.2024.104103","url":null,"abstract":"<div><h3>Research question</h3><p>Does double blastocyst vitrification and warming affect pregnancy, miscarriage or live birth rates, or birth outcomes, from embryos that have undergone preimplantation genetic testing for aneuploidies (PGT-A) testing?</p></div><div><h3>Design</h3><p>This retrospective observational analysis of embryo transfers was performed at a single centre between January 2017 and August 2022. The double-vitrification group included frozen blastocysts that were vitrified after 5–7 days of culture, warmed, biopsied (either once or twice) and re-vitrified. The single vitrification (SV) group included fresh blastocysts that were biopsied at 5–7 days and then vitrified.</p></div><div><h3>Results</h3><p>A comparison of the 84 double-vitrification blastocysts and 729 control single-vitrification blastocysts indicated that the double-vitrification embryos were frozen later in development and had expanded more than the single-vitrification embryos. Of the 813 embryo transfer procedures reported, 452 resulted in the successful delivery of healthy infants (56%). There were no significant differences between double-vitrification and single-vitrification embryos in the pregnancy, miscarriage or live birth rates achieved after single-embryo transfer (55% versus 56%). Logistic regression indicated that while reduced live birth rates were associated with increasing maternal age at oocyte collection, longer culture prior to freezing and lower embryo quality, double vitrification was not a significant predictor of live birth rate.</p></div><div><h3>Conclusions</h3><p>Blastocyst double vitrification was not shown to impact pregnancy, miscarriage or live birth rates. Although caution is necessary due to the study size, no effects of double vitrification on miscarriage rates, birthweight or gestation period were noted. These data offer reassurance given the absence of the influence of double vitrification on all outcomes after PGT-A.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141036570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoplasmic reticulum stress-mediated ferroptosis in granulosa cells contributes to follicular dysfunction of polycystic ovary syndrome driven by hyperandrogenism","authors":"","doi":"10.1016/j.rbmo.2024.104078","DOIUrl":"10.1016/j.rbmo.2024.104078","url":null,"abstract":"<div><h3>Research question</h3><p>Does hyperandrogenaemia affect the function of ovarian granulosa cells by activating ferroptosis, and could this process be regulated by endoplasmic reticulum stress?</p></div><div><h3>Design</h3><p>Levels of ferroptosis and endoplasmic reticulum stress in granulosa cells were detected in women with and without polycystic ovary syndrome (PCOS) undergoing IVF. Ferroptosis and endoplasmic reticulum stress levels of ovarian tissue and follicle development were detected in control mice and PCOS-like mice models, induced by dehydroepiandrosterone. An in-vitro PCOS model of KGN cells was constructed with testosterone and ferroptosis inhibitor Fer-1. Endoplasmic reticulum stress inhibitor, tauroursodeoxycholate (TUDCA), determined the potential mechanism associated with excessive induction of ferroptosis in granulosa cells related to PCOS, and levels of ferroptosis and endoplasmic reticulum stress were detected.</p></div><div><h3>Results</h3><p>Activation of ferroptosis and endoplasmic reticulum stress occurred in granulosa cells of women with PCOS and the varies of PCOS-like mice. The findings in KGN cells demonstrated that testosterone treatment results in elevation of oxidative stress levels, particularly lipid peroxidation, and intracellular iron accumulation in granulosa cells. The expression of genes and proteins associated with factors related to ferroptosis, mitochondrial membrane potential and ultrastructure showed that testosterone activated ferroptosis, whereas Fer-1 reversed these alterations. During in-vitro experiments, activation of endoplasmic reticulum stress induced by testosterone treatment was detected in granulosa cells. In granulosa cells, TUDCA, an inhibitor of endoplasmic reticulum stress, significantly mitigated testosterone-induced ferroptosis.</p></div><div><h3>Conclusions</h3><p>Ferroptosis plays a part in reproductive injury mediated by hyperandrogens associated with PCOS, and may be regulated by endoplasmic reticulum stress.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472648324002670/pdfft?md5=69ada9461d673236b6edf83867fe1033&pid=1-s2.0-S1472648324002670-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141029374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Good practice in clinical embryology laboratories: Association of Reproductive and Clinical Scientists Guidelines 2024","authors":"","doi":"10.1016/j.rbmo.2024.104102","DOIUrl":"10.1016/j.rbmo.2024.104102","url":null,"abstract":"<div><div>Clinical embryology is a dynamic and ever-evolving field, and as such clinical practice guidelines must be regularly reviewed and updated. Accordingly, this document supersedes previous good clinical practice in clinical embryology guidance, developing, and updating where necessary, existing good practice guidelines. The Association of Reproductive and Clinical Scientists (ARCS) suggests this Good Laboratory Practice framework as appropriate and effective to ensure the best possible care for all individuals undertaking fertility treatment. Efforts have been made to ensure that the language used is inclusive and reflective of the wide range of patients who seek treatment. The guidance provided within this document aims to be relevant within an international context and to consider the range of regulatory considerations affecting clinical embryology laboratories. This guideline was written to guide best practice but is not intended as a tool to judge the practice of centres within the UK or beyond.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141037385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attitude of Belgian women towards enucleated egg donation for treatment of mitochondrial diseases and infertility","authors":"Guido Pennings ,&nbsp;Björn Heindryckx ,&nbsp;Dominic Stoop ,&nbsp;Heidi Mertes","doi":"10.1016/j.rbmo.2024.104101","DOIUrl":"10.1016/j.rbmo.2024.104101","url":null,"abstract":"<div><h3>Research question</h3><p>What is the attitude of Belgian women of reproductive age towards enucleated egg donation? Does the willingness of women to donate differ when they would donate enucleated or whole eggs?</p></div><div><h3>Design</h3><p>In 2022, an online survey was conducted among a representative sample of 1000 women in Belgium aged 18–50 years. The item on willingness to anonymously donate enucleated eggs was dichotomized into those willing to donate and those not willing to donate or uncertain.</p></div><div><h3>Results</h3><p>No statistically significant difference was found between the willingness to donate enucleated eggs and whole eggs (whether anonymously or identifiably). Anonymity, however, affected the willingness to donate, with considerably fewer women willing to donate identifiably. The respondents were divided about their parental status if they were to donate enucleated eggs, with less than one-half (44%) not considering themselves to be a genetic mother. Women willing to donate enucleated eggs anonymously were less likely to view themselves as a genetic mother of the child compared with others. Fewer than one in five considered the technique unacceptable because the resulting child would carry genetic material of three persons.</p></div><div><h3>Conclusions</h3><p>Women in the general population did not show a greater willingness to donate enucleated eggs than whole eggs. The fact that the respondents were strongly divided on whether or not they would consider themselves to be a genetic mother of the resulting child may explain this result. Other factors, such as the potential high risk for the child, may also have contributed to less willingness.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141030232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of inclusion of a poor-quality embryo with a good-quality embryo on pregnancy outcomes in vitrified-warmed blastocyst transfers","authors":"","doi":"10.1016/j.rbmo.2024.104104","DOIUrl":"10.1016/j.rbmo.2024.104104","url":null,"abstract":"<div><h3>Research question</h3><p>Does the co-transfer of a good-quality embryo and a poor-quality embryo influence pregnancy outcomes in comparison to the transfer of a single good-quality embryo in vitrified-warmed blastocyst transfer cycles?</p></div><div><h3>Design</h3><p>This retrospective cohort study involved a total of 11,738 women who underwent IVF/intracytoplasmic sperm injection cycles and vitrified-warmed blastocyst transfer at a tertiary-care academic medical from January 2015 to June 2022. The study population was categorized into two groups: single-blastocyst transfer (SBT; participants who underwent single good-quality embryo transfer, <em>n</em> = 9338) versus double-blastocyst transfer (DBT; participants who underwent transfers with a poor and a good-quality embryo, <em>n</em> = 2400).</p></div><div><h3>Results</h3><p>The live birth rate (LBR) was significantly higher in the DBT group in comparison with the SBT group (65.6% versus 56.3%, <em>P</em> &lt; 0.001). Multivariable logistic regression analysis showed that DBT was an independent predictor for LBR with a strong potential impact (adjusted odds ratio 1.55, 95% confidence interval 1.41–1.71; <em>P</em> &lt; 0.001). However, the multiple birth rate was significantly higher in the good-quality embryo and poor-quality embryo group compared with patients undergoing a single good-quality embryo transfer (41.4% versus 1.8%; <em>P</em> &lt; 0.001).</p></div><div><h3>Conclusions</h3><p>In vitrified-warmed blastocyst transfer cycles, LBR was higher following DBT with one good-quality and one poor-quality embryo compared with SBT. However, this was at the expense of a marked increase in the likelihood of multiple gestations. Physicians should still balance the benefits and risks of double-embryo transfer.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141047085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pain after oocyte retrieval in women with endometriosis undergoing fertility preservation or IVF","authors":"Maha Eid ,&nbsp;Adrien Lemoine ,&nbsp;Lena Bardet ,&nbsp;Lise Selleret ,&nbsp;Sophie Stout ,&nbsp;Emmanuelle Mathieu d'Argent ,&nbsp;Anna Ly ,&nbsp;Nathalie Sermondade ,&nbsp;Cyril Touboul ,&nbsp;Charlotte Dupont ,&nbsp;Nathalie Chabbert-Buffet ,&nbsp;Kamila Kolanska","doi":"10.1016/j.rbmo.2024.104100","DOIUrl":"10.1016/j.rbmo.2024.104100","url":null,"abstract":"<div><h3>Research question</h3><p>Do women with endometriosis undergoing oocyte retrieval for fertility preservation experience the same level of pain as women undergoing oocyte retrieval for IVF?</p></div><div><h3>Design</h3><p>This retrospective cohort study included 796 cycles in women with endometriosis undergoing oocyte retrieval for fertility preservation (<em>n</em> = 401) or IVF (<em>n</em> = 395) between January 2020 and October 2022. Post-operative pain assessments were compared between the two groups using a numeric rating scale (NRS).</p></div><div><h3>Results</h3><p>Women in the fertility preservation group were younger (32.1 ± 4.2 years versus 35.1 ± 4.1 years; <em>P</em> &lt; 0.001), had a lower body mass index (22.8 ± 3.9 kg/m² versus 24.6 ± 4.4 kg/m²; <em>P</em> &lt; 0.001) and had a lower concentration of anti-Müllerian hormone (1.8 ± 1.5 ng/ml versus 2.15 ± 2.11 ng/ml; <em>P</em> = 0.026) in comparison with women in the IVF group. The oestrogen concentration on the day of ovulation trigger was higher in women in the fertility preservation group (2188 ± 1152 pg/ml versus 2081 ± 995 pg/ml; <em>P</em> = 0.004), and the prevalence rates of adenomyosis and digestive endometrial lesions were lower in women in the fertility preservation group (14% versus 29%, <em>P</em> &lt; 0.001; 16% versus 25%, <em>P</em> = 0.003, respectively) compared with women in the IVF group. After oocyte puncture, more women in the fertility preservation group had an NRS pain score &gt;3 (moderate to severe pain) compared with women in the IVF group (20% versus 14%; <em>P</em> = 0.018). The progestin-primed ovarian stimulation (PPOS) protocol was identified as an independent predictive factor of greater post-operative pain (adjusted OR 2.30, 95% CI 1.06–5.15; <em>P</em> = 0.039).</p></div><div><h3>Conclusion</h3><p>Women with endometriosis undergoing fertility preservation reported more intense post-operative pain in the recovery room than women undergoing IVF. The PPOS protocol was an independent risk factor of intense pain (NRS pain score &gt;3) in women with endometriosis, but further studies are needed to confirm this result.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141027838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between visceral adipose tissue area and infertility: a cross-sectional analysis","authors":"Zhiyang Zhou ,&nbsp;Yue Xu ,&nbsp;Gaochen Zhang ,&nbsp;Peiran Hu ,&nbsp;Weihui Shi ,&nbsp;Sisi Zhang ,&nbsp;Jiexue Pan","doi":"10.1016/j.rbmo.2024.104099","DOIUrl":"10.1016/j.rbmo.2024.104099","url":null,"abstract":"<div><h3>Research question</h3><p>Is intra-abdominal fat obesity associated with infertility?</p></div><div><h3>Design</h3><p>This study analysed data from the 2013–2018 National Health and Nutrition Examination Survey, with a total of 3013 women enrolled. The participants were divided into two groups: infertility and non-infertility. Differences between the two groups were analysed using a weighted Student's <em>t</em>-test or Mann–Whitney <em>U</em>-test for continuous variables, or a weighted chi-squared test for categorical data. Visceral adipose tissue area (VATA) was assessed by dual-energy X-ray absorptiometry. The independent association between infertility and log VATA was assessed by weighted multivariate logistic regression models. Subgroup analyses were performed to assess the strength of the results. Interaction tests were used to examine whether covariates interacted with log VATA to influence infertility.</p></div><div><h3>Results</h3><p>Log VATA was significantly higher in the infertility group compared with the non-infertility group (<em>P</em> &lt; 0.001). After adjustment for potential confounders, the results of multivariate logistic regression analysis revealed that an increase in log VATA was associated with increased prevalence of female infertility (OR = 2.453, 95% CI 1.278–4.792). Subgroup analyses showed this association in individuals aged &lt;35 years (<em>P</em> = 0.002), Mexican-Americans (<em>P</em> = 0.033), non-hypertensive individuals (<em>P</em> = 0.013) and non-diabetic individuals (<em>P</em> = 0.003).</p></div><div><h3>Conclusions</h3><p>An enlarged VATA is associated with increased risk of infertility. The direct effect of VATA on female infertility needs to be clarified further to provide a basis for future prevention and treatment of female infertility.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141023221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Day of blastulation correlates with ploidy status and serves as a useful non-invasive parameter for ranking embryos from non-PGT-A patients for transfer","authors":"K. Willoughby ,&nbsp;L. Moffatt ,&nbsp;T. Neal ,&nbsp;M. Tovar ,&nbsp;S. Amin ,&nbsp;S. Deniz ,&nbsp;M. Faghih ,&nbsp;M.F. Karnis ,&nbsp;M.S. Neal","doi":"10.1016/j.rbmo.2024.104122","DOIUrl":"https://doi.org/10.1016/j.rbmo.2024.104122","url":null,"abstract":"<div><h3>BACKGROUND AND AIM</h3><p>Embryologists continue to strive to establish non-invasive parameters for ranking embryos to prioritize the ones with the highest chance of pregnancy success. Embryo morpho-kinetics have been correlated with implantation success. Previous studies have demonstrated that day 5 blastocysts result in higher implantation and pregnancy success as compared to day 6 blastocysts [1,2]. We aimed to test the hypothesis that embryos reaching an expanded blastocyst stage on day 5 are more likely to be euploid compared to their day 6 counterparts.</p></div><div><h3>METHODS</h3><p>We conducted a blinded retrospective review of patients undergoing PGT-A. Information from 253 women, aged 25 to 44, undergoing IVF/ICSI and PGT-A from January 1, 2022, to December 31, 2023, from which n = 1161 embryos were biopsied and PGT-A tested by Next Generation Sequencing (NGS) and compared. Chi-square analysis was conducted to determine differences between the groups with a p-value &lt;0.05 considered statistically significant.</p></div><div><h3>RESULTS</h3><p>Of the 1161 biopsied and PGT-A tested embryos, 688 were day 5 blastocysts and 466 reached the blastocyst stage on day 6. The remaining 7 embryos were expanded blastocysts on day 7. The proportion of euploid embryos among day 5 blastocysts (49.4%) was significantly greater (p&lt;0.0001) than that of day 6 blastocysts (32.8%) – Table 1. Corresponding aneuploidy rates were lower (p&lt;0.0001) on day 5 (31.1%) compared to day 6 (45.3%). There was not any difference between High Level Mosaics (HLM); Low Level Mosaics (LLM) or no result reports observed (Figure 1).</p></div><div><h3>CONCLUSIONS</h3><p>Our results suggest that the genetic composition of an embryo is associated with the rate of embryo development, with day 5 blastocysts demonstrating a significantly higher likelihood of being euploid. This information is clinically important and provides valuable, non-invasive criteria regarding embryo quality, which can be used in embryo selection to improve clinical outcomes in non-PGT-A patients.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141163467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm-derived Centrioles are Essential Contributors to Accurate Chromosome Segregation in the Initial Mitotic Division of Mammalian Zygotes","authors":"A. Larreategui Aparicio ,&nbsp;I. Barale ,&nbsp;C. Deelen ,&nbsp;T.A. Stout ,&nbsp;G.J. Kops ,&nbsp;M. de Ruijter-Villani","doi":"10.1016/j.rbmo.2024.104026","DOIUrl":"https://doi.org/10.1016/j.rbmo.2024.104026","url":null,"abstract":"<div><h3>BACKGROUND AND AIM</h3><p>The centrosomes are the major microtubule-organizing centers in somatic cells and have an essential role in spindle assembly and chromosome segregation. Centrioles are also found in the zygotes, but they are introduced by the spermatozoon at fertilization and they are structurally different to the canonical centrioles. We recently demonstrated that centrosomes play a minor role during zygotic spindle assembly, but its role in chromosome segregation remains unknown. The aim of this study is to investigate if centrosome malpositioning or inactivity predisposes to chromosome segregation errors and failure of cytokinesis.</p></div><div><h3>METHODS</h3><p>Bovine oocytes were retrieved from slaughterhouse ovaries and matured in vitro. Matured oocytes were fertilized in vitro with semen from a bull of proven fertility and, either fixed at 27-29h post-fertilization, or used for time-lapse light-sheet imaging after injection of mRNA for microtubule and chromatin reporters. Fixed bovine zygotes were immunostained for chromatin, alpha-tubulin, centriolar and pericentriolar components, and imaged with a confocal microscope. Microtubule nucleation ability was evaluated using a microtubule regrowth assay.</p></div><div><h3>RESULTS</h3><p>Live imaging showed that in 52% of bovine zygotes (24/46) the centrosomes engaged with the spindle but were malpositioned, while in 26% (12/46) the centrosomes were not visible (inactive). All zygotes with normally positioned centrosomes showed correct chromosome segregation, whereas 44% of zygotes with abnormal centriole positioning showed lagging chromosomes and the formation of micronuclei.</p><p>When centriole duplication was prevented, the sperm-derived centrioles showed a similar ability to recruit pericentrosomal material (NEDD1) and nucleate microtubules. However, live imaging showed that in 82% (14/17) of PLK4i treated zygotes the spindle, although bipolar, was unusually elongated and chromosomes either failed to align correctly on the metaphase plate or showed anaphase lag. Moreover, in treated zygotes where both centrioles were positioned at the same spindle pole, the spindle failed to anchor correctly to the cell membrane, resulting in rotational behavior of the zygote and an increase in cytokinesis failure of up to 47% (8/17).</p></div><div><h3>CONCLUSIONS</h3><p>These results show how correct centrosome positioning guarantees spindle stability and correct chromosome segregation. On the other hand, when centrosomes are malpositioned or inactive, there is an increase in chromosome segregation errors and failure of cytokinesis.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141163274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Usefulness of morphokinetics in early stages of embryonic development as a predictive value of blastocyst formation and quality","authors":"S. Camacho Fernandez Pacheco ,&nbsp;L. Magro Bello ,&nbsp;R. Barba Martin ,&nbsp;S. Bartolome Sotillos ,&nbsp;M. Duro Carave ,&nbsp;I. Fares Bejarano ,&nbsp;H. Frias Maroto ,&nbsp;A. Perdiguero Menendez ,&nbsp;C. Fernandez Manso ,&nbsp;C. Franganillo Lopez De La Manzanara ,&nbsp;B. Acevedo Martin","doi":"10.1016/j.rbmo.2024.104014","DOIUrl":"https://doi.org/10.1016/j.rbmo.2024.104014","url":null,"abstract":"<div><h3>BACKGROUND AND AIM</h3><p>Although culture to blastocyst has been associated with an increase in implantation rates, sometimes this is not possible due to multiple casuistry. Thus, trying to relate morphokinetics in early stages with the capacity to form blastocysts and their quality can be very useful. Timelapse incubators help to establish the exact moment in which blastomere divisions occur, evaluating embryo morphology in a non-invasive way and allowing a better selection of embryos to be transferred and/or vitrified. The aim of this study is to evaluate the predictive value of t4 (hours elapsed from insemination to 4-cell division) in the formation of blastocysts and their quality.</p></div><div><h3>METHODS</h3><p>Retrospective study with 385 blastocysts from patients undergoing IVF-ICSI treatment in our hospital from June to December 2023. There are no significant differences, neither in age nor in stimulation protocols, in the different groups. Both ICM and TM were classified based on the ASEBIR proposal. Statistical analysis by ANOVA for mean comparison.</p></div><div><h3>RESULTS</h3><p>Significant differences (p value = 0.000) were observed when comparing T4 between embryos that did not reach blastocyst (47.9 ±19.55 min) and those that did (37.9 ±10.31min). No significant differences were observed in T4 in terms of quality of useful blastocysts (A: 36.34; B: 36.46 and C: 36.35). In contrast, a certain tendency to compact morula formation in shorter time is observed with higher final blastocyst quality (A: 83.56; B: 84.72 and C: 87.46).</p></div><div><h3>CONCLUSIONS</h3><p>T4 has a high potential as a predictive value in blastocyst formation and, consequently, gestation, allowing us to determine which embryos are best to transfer and/or cryopreserve.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141163333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}