Reproductive biomedicine online最新文献

{"title":"Effort in manual operation of reproductive health cryostorage facilities","authors":"Alicia Broussard ,&nbsp;Bill Venier ,&nbsp;Abeer Salhia Rafati ,&nbsp;Angeline Beltsos ,&nbsp;Joseph Lee ,&nbsp;Jessica Bailey ,&nbsp;Denny Sakkas ,&nbsp;Michael G. Collins","doi":"10.1016/j.rbmo.2024.104348","DOIUrl":"10.1016/j.rbmo.2024.104348","url":null,"abstract":"<div><h3>Research question</h3><p>What areas of manual IVF cryostorage operations are common to the safe operation of IVF cryostorage facilities and require effort from embryologists?</p></div><div><h3>Design</h3><p>Observational time and motion data were collected by two observers equipped with the digital cameras over 2 weeks at four well-characterized US IVF centres (sites α, β, γ and δ) from 12 participants performing cryostorage tasks. To understand the work processes of the different sites and assist in the data analysis, informal interviews were conducted with the study participants and laboratory directors. Data were analysed to identify work processes that might be eliminated or diminished by automation and software improvements.</p></div><div><h3>Results</h3><p>On average, it took 3.4 data record queries per retrieval from cryostorage to identify a cane, while the canister was lifted an average of 1.5 times per retrieval, with a mean 11.8 ± 9.2 s per lift. Of the total time spent working with cryostorage equipment, 47.25% was of a fatiguing nature. Sites α, β and γ utilized one person to fill the liquid nitrogen storage Dewars, while site δ had two technicians working in tandem to move and fill the Dewars, with different frequencies and determination factors for refills and efficiencies.</p></div><div><h3>Conclusions</h3><p>This time and motion study demonstrated significant time investment, task redundancy and fatiguing working conditions among embryologists using manual cryostorage processes. There was a disparity of processes and space capacity across different laboratories. Some of these issues may be addressed by the integration of automation and technology solutions.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 5","pages":"Article 104348"},"PeriodicalIF":3.7,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472648324005376/pdfft?md5=42e95bffc126b0109ab7cca1a06dbc58&pid=1-s2.0-S1472648324005376-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142098791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial oocyte activation improves ICSI outcomes following unexplained fertilization abnormalities","authors":"C.L. Nicholson ,&nbsp;M. Dean ,&nbsp;A. Attia ,&nbsp;P.A. Milne ,&nbsp;S. Martins da Silva","doi":"10.1016/j.rbmo.2024.104327","DOIUrl":"10.1016/j.rbmo.2024.104327","url":null,"abstract":"<div><h3>Research question</h3><p>Is artificial oocyte activation (AOA) effective for patients with unexplained low or no fertilization following IVF/intracytoplasmic sperm injection (ICSI)?</p></div><div><h3>Design</h3><p>All IVF/ICSI cases resulting in total fertilization failure or fertilization rate ≤25% at Ninewells Assisted Conception Unit, Dundee between January 2014 and December 2021 (<em>n</em> = 231) were reviewed contemporaneously. After exclusion of obvious stimulation, egg, sperm and/or assisted reproductive technology laboratory factors, patients with at least one cycle of IVF/ICSI resulting in apparently unexplained fertilization abnormalities were offered research investigations, including sperm immunocytochemistry for phospholipase C zeta (PLCζ) protein expression. This retrospective case–control cohort study evaluated laboratory and clinical outcomes for 39 couples (15 attended for sperm studies research) that subsequently undertook ICSI-AOA with Ca²⁺ ionophore.</p></div><div><h3>Results</h3><p>Comparing preceding IVF/ICSI and subsequent ICSI-AOA for each patient, the number of eggs collected was similar; however, ICSI-AOA resulted in a significantly improved fertilization rate (57.2% versus 7.1%; <em>P</em> &lt; 0.0001). The uplift for a subset of 10 patients identified with PLCζ deficiency was 66.3% versus 4.6% (<em>P</em> &lt; 0.0001). Overall, ICSI-AOA resulted in a higher number of fresh embryo transfers (94.6% versus 33.3%; <em>P</em> &lt; 0.0001), a significantly higher clinical pregnancy rate (CPR) and live birth rate (LBR; 18.9% versus 2.6%; <em>P</em> = 0.02), a significant increase in cycles with surplus embryos suitable for cryostorage (43.6% versus 0%; <em>P</em> &lt; 0.0001), and increased cumulative CPR (41.0% versus 2.6%; <em>P</em> &lt; 0.0001) and LBR (38.5% versus 2.6%; <em>P</em> &lt; 0.0001).</p></div><div><h3>Conclusion</h3><p>AOA is a powerful tool that can transform clinical outcomes for couples experiencing apparently unexplained fertilization abnormalities. PLCζ assays have the potential to be valuable diagnostic tools to determine patient selection for ICSI-AOA, and research efforts should continue to focus on their development.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 5","pages":"Article 104327"},"PeriodicalIF":3.7,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472648324005169/pdfft?md5=703e610a34e64934d0cab63c4a41c609&pid=1-s2.0-S1472648324005169-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142146148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between fresh single embryo morphology scores and serum HCG values at 14 days and 14–18-day doubling values","authors":"Junting Chu,&nbsp;Shanyue Guan,&nbsp;Ruonan Ma,&nbsp;Xiangyang Zhang,&nbsp;Song Ning,&nbsp;Wenyan Song,&nbsp;Guidong Yao,&nbsp;Senlin Shi,&nbsp;Haixia Jin","doi":"10.1016/j.rbmo.2024.104325","DOIUrl":"10.1016/j.rbmo.2024.104325","url":null,"abstract":"<div><h3>Research question</h3><p>What is the effect of embryo morphology score on 14-day β-HCG levels and 14–18-day β-HCG doubling values, and do they have differences in day-3 embryo or day-5 blastocyst transfers?</p></div><div><h3>Design</h3><p>Retrospective analysis of 4434 fresh cycles of single embryo transfers (SET) with β-HCG ≥15 mIU/ml on day 14 after transfer via IVF and ICSI. The correlation between embryo morphology score and 14-day β-HCG was examined. Doubling of 14–18 day β-HCG was analysed in 2628 cycles to determine correlations with embryo morphology score.</p></div><div><h3>Results</h3><p>In day-3 SET, number of embryonic cells was positively correlated with 14-day post-transfer β-HCG values (R = 0.076; <em>P</em> = 0.013). No significant correlation was observed between the grade of the transferred embryos and the 14–18-day serum β-HCG doubling values. In day-5 single blastocyst transfers, the degree of blastocyst expansion, trophoblast cell and inner cell mass (ICM) grades demonstrated a significant positive correlation with 14-day post-transfer β-HCG (<em>P</em> &lt; 0.001, <em>P</em> = 0.014, <em>P</em> = 0.003). Degree of blastocyst expansion was significantly correlated with 14–18-day β-HCG doubling values (R = –0.051, <em>P</em> = 0.027). Grades of the ICM and trophoblast cells showed no significant correlation with 14–18-day β-HCG doubling values.</p></div><div><h3>Conclusion</h3><p>In fresh SET, embryo morphology score influences 14-day β-HCG values in day-3 embryos and day-5 blastocyst transfers. Embryo morphology score in day-3 SET does not affect 14–18-day β-HCG doubling values. Degree of blastocyst expansion significantly affects 14–18-day β-HCG doubling values in day-5 blastocyst transfers.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 5","pages":"Article 104325"},"PeriodicalIF":3.7,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142098792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A precise and cost-efficient whole-genome haplotyping method without probands: preimplantation genetic testing analysis","authors":"Zhiqiang Zhang ,&nbsp;Kai Kang ,&nbsp;Linan Xu ,&nbsp;Xiaolan Li ,&nbsp;Shujing He ,&nbsp;Ruixia Xu ,&nbsp;Lei Jia ,&nbsp;Shihui Zhang ,&nbsp;Wenlong Su ,&nbsp;Peng Sun ,&nbsp;Mengnan Gu ,&nbsp;Wenqi Shan ,&nbsp;Yawen Zhang ,&nbsp;Lingyin Kong ,&nbsp;Bo Liang ,&nbsp;Cong Fang ,&nbsp;Zi Ren","doi":"10.1016/j.rbmo.2024.104328","DOIUrl":"10.1016/j.rbmo.2024.104328","url":null,"abstract":"<div><h3>Research question</h3><div>Is there a precise and efficient haplotyping method to expand the application of preimplantation genetic testing (PGT)?</div></div><div><h3>Design</h3><div>In this study, eight cell-line families and 18 clinical families including 99 embryos were used to construct whole-genome haplotyping based on link-read sequencing (Phbol-seq) and optimized analytical workflow with a correction algorithm. The correction algorithm was based on a differentiation of assembly errors and homologous recombination, in which the main feature of parental assembly error was that all embryos (embryo number ≥2) had breakpoints at the same chromosome position.</div></div><div><h3>Results</h3><div>With Phbol-seq, parental assembly errors and homologous recombination were accurately distinguished and corrected. Using the link-reads (&gt;25% long-reads were ≥30 kilobases [kb]), complete genome-wide parental haplotypes were constructed, and the consistency of the typing results of each chromosome with a conventional method requiring other family members was more than 95%. In addition, the length of N50 contigs was 11.03–16.2 million bases (mb), which was far beyond the N50 contigs from long-read sequencing (148–863 kb). The complete haplotype analysis of all embryos could be performed by Phbol-seq and revealed 100% concordance with the available diagnostic results obtained by the conventional method requiring other family members.</div></div><div><h3>Conclusions</h3><div>: Phbol-seq has high clinical value as a precise and cost-efficient whole-genome haplotyping method without probands as part of PGT and other genetic research, which could promote the application of PGT to decrease the birth of children with genetic diseases and the development of linkage-related genetic research.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 1","pages":"Article 104328"},"PeriodicalIF":3.7,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into molecular mechanisms underlying malignant transformation of endometriosis: BANCR promotes miR-612/CPNE3 pathway activity","authors":"Chang Liu ,&nbsp;Peng Chen ,&nbsp;Zhuo Yang ,&nbsp;Keming Zhang ,&nbsp;Fang Chen ,&nbsp;Yanmei Zhu ,&nbsp;Jing Liu ,&nbsp;Liying Liu ,&nbsp;Danni Wang ,&nbsp;Danbo Wang","doi":"10.1016/j.rbmo.2024.104326","DOIUrl":"10.1016/j.rbmo.2024.104326","url":null,"abstract":"<div><h3>Research question</h3><div>Does LncRNA <em>BANCR</em> promote the malignant transformation of endometriosis by activating the miR-612/CPNE3 pathway?</div></div><div><h3>Design</h3><div>The expression patterns of <em>BANCR</em>, miR-612 and <em>CPNE3</em> in normal endometrium, eutopic endometrium from endometriosis, eutopic endometrium or malignant tissues from endometriosis-associated ovarian cancer. On the basis of primary normal endometrial stromal cells (NESC) and eutopic endometrial stromal cells (EESC), the regulatory relationships between <em>BANCR</em>, miR-612 and <em>CPNE3</em>, and the potential mechanisms that promote the malignant transformation of endometriosis, were elucidated <em>in vitro</em> and <em>in vivo</em>.</div></div><div><h3>Results</h3><div>The expression levels of <em>BANCR</em> and <em>CPNE3</em> were lowest in normal endometrium, significantly increased in eutopic endometrium (<em>P</em> &lt; 0.05) and was significantly increased in eutopic endometrium (<em>P</em> &lt; 0.05). During the malignant transformation of endometriosis, the expression levels of <em>BANCR</em> and CPNE3 were significantly upregulated (<em>P</em> &lt; 0.05), whereas those of miR-612 were significantly downregulated (<em>P</em> &lt; 0.05). miRNA-612 was found to target <em>BANCR</em> and <em>CPNE3</em>. The overexpression and knockdown of <em>BANCR</em> in NESC and EESC upregulated and downregulated the expression of CPNE3 and promoted or prevented cell proliferation and migration, respectively; these effects were reversed by miR-612 mimics and inhibitor. These changes were all statistically significant (<em>P</em> &lt; 0.05). In-vivo experiments revealed that <em>BANCR</em> significantly increased the survival of subcutaneous endometrial cells by regulating miR-612/CPNE3 (<em>P</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>The expression of <em>BANCR</em> gradually increased with the progression of endometriosis during malignant transformation, and promoted the proliferation and migration of endometrial cells via the miR-612/CPNE3 pathway. <em>BANCR</em> may represent a novel target for monitoring the malignant transformation of endometriosis.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 6","pages":"Article 104326"},"PeriodicalIF":3.7,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm penetration at the maturing metaphase I stage can trigger oocyte activation in a mouse model","authors":"Ching-Chien Chang ,&nbsp;Min Peng ,&nbsp;Li-Kuang Tsai ,&nbsp;Chia-Chun Chang ,&nbsp;Chia-Jung Li ,&nbsp;Chia-Kuan Wu ,&nbsp;Chin-Cheng Chien ,&nbsp;Jie Xu ,&nbsp;Zsolt Peter Nagy ,&nbsp;Chi-Hong Liu ,&nbsp;Chung-Hao Lu ,&nbsp;Li-Ying Sung","doi":"10.1016/j.rbmo.2024.104329","DOIUrl":"10.1016/j.rbmo.2024.104329","url":null,"abstract":"<div><h3>Research question</h3><div>Can spermatozoa penetrate maturing metaphase I (MI) oocytes, and render subsequent development following conventional IVF in a mouse model?</div></div><div><h3>Design</h3><div>ICR mice were used in this study. Metaphase II (MII) cumulus–oocyte complexes (COC) harvested 15 h after injection of human chorionic gonadotrophin (HCG) were used for IVF as the control group (Group 1). In the treatment group (Group 2), maturing MI COC harvested 7 h after HCG injection were used for IVF. Fertilization, pronuclear formation, cleavage, blastocyst formation, DNA methylation status, chromosome number and live birth rates were used to evaluate the developmental dynamics and competency of maturing MI oocytes following conventional IVF.</div></div><div><h3>Results</h3><div>Maturing MI COC were fertilized using conventional IVF, and sperm penetration at MI–telophase I triggered oocyte activation. Most embryos resulting from fertilized MI oocytes developed to blastocyst stage during preimplantation development, albeit a substantial proportion of them were triploids due to the absence of the second meiotic division. Some of the embryos derived from fertilization of maturing oocytes were able to implant and gave rise to full-term development.</div></div><div><h3>Conclusion</h3><div>Maturing MI COC from follicles before ovulation could be used for mouse IVF, and fertilized MI oocytes had high potential for development. Healthy offspring can be generated from maturing MI COC following conventional IVF. MI COC may represent a valuable source of ‘usable’ biomaterial in assisted reproduction. However, many embryos derived from MI COC via IVF have abnormal chromosome numbers in the mouse model. The implications of these findings for human IVF remain to be investigated.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 6","pages":"Article 104329"},"PeriodicalIF":3.7,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential anatomical determinants of retrograde menstruation: a comprehensive narrative review","authors":"Paolo Vercellini ,&nbsp;Martina Piccini ,&nbsp;Francesca Caprara ,&nbsp;Giulia Emily Cetera ,&nbsp;Paola Viganò ,&nbsp;Edgardo Somigliana","doi":"10.1016/j.rbmo.2024.104345","DOIUrl":"10.1016/j.rbmo.2024.104345","url":null,"abstract":"<div><p>A century ago, Sampson identified three uterine anatomical structures that may determine the amount of retrograde menstruation and the likelihood of the development of endometriosis: the cervix, the intramural portion of the fallopian tubes, and the myometrium. Critical appraisal was undertaken of data published over the last 40 years on the potential effect of the characteristics of these three anatomical variables on the risk of endometriosis. There is some evidence to support the pathogenic role of the diameter of the cervical canal, stenosis of internal or external orifices, and stiffness of cervical tissue. One study showed a significant association between the morphology of the intramural tubal tract and the frequency of endometriosis. A large body of evidence points to abnormalities of the myometrial structure as the anatomical aberration most consistently associated with endometriosis. These abnormalities have largely been interpreted as signs of early-onset adenomyosis, which may precede endometriosis and even lead to its development by increasing the amount of retrograde menstruation. Future research should aim to verify whether a positive relationship exists between the substantially increased number of ovulatory menses occurring in the decade following menarche, the development of anatomical myometrial abnormalities, changes in the amount of retrograde menstruation over time, and the risk of endometriosis.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 4","pages":"Article 104345"},"PeriodicalIF":3.7,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141933649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atorvastatin improves ovarian function and follicular reserve in rats with premature ovarian insufficiency","authors":"Parmis Notghi,&nbsp;Malek Soleimani Mehranjani,&nbsp;Seyed Mohammad Ali Shariatzadeh","doi":"10.1016/j.rbmo.2024.104324","DOIUrl":"10.1016/j.rbmo.2024.104324","url":null,"abstract":"<div><h3>Research question</h3><p>Can atorvastatin, with its antioxidant, anti-inflammatory and anti-apoptotic properties, improve ovarian function and follicular reserve in rats with cyclophosphamide-induced premature ovarian insufficiency (POI)?</p></div><div><h3>Design</h3><p>In this experimental study, 24 adult female Wistar rats were divided into four groups: control; POI; POI + atorvastatin; and atorvastatin. After treatment with atorvastatin, serum concentrations of total antioxidant capacity, glutathione, malondialdehyde, FSH, oestradiol, anti-Müllerian hormone, tumour necrosis factor-alpha and interleukin-6 were evaluated. Additionally, mRNA and protein expression of Bax, Bcl-2 and VEGF-A; number of follicles; and total volume of the ovary, and volumes of the cortex and medulla were examined.</p></div><div><h3>Results</h3><p>The results showed that serum concentrations of total antioxidant capacity (<em>P</em> &lt; 0.001), glutathione, oestradiol and anti-Müllerian hormone (<em>P</em> &lt; 0.05); mRNA and protein expression of Bcl-2 and VEGF-A (<em>P</em> &lt; 0.05); number of primordial and primary follicles (<em>P</em> &lt; 0.001), and preantral and antral follicles (<em>P</em> &lt; 0.01); and total volume of the ovary, and volume of the cortex (<em>P</em> &lt; 0.05) increased significantly in the POI + atorvastatin group compared with the POI group. Serum concentrations of malondialdehyde, FSH, tumour necrosis factor-alpha and interleukin-6; and mRNA and protein expression of Bax decreased significantly in the POI + atorvastatin group compared with the POI group (<em>P</em> &lt; 0.05).</p></div><div><h3>Conclusions</h3><p>Atorvastatin reduces the detrimental effects of cyclophosphamide in the POI model significantly by reducing oxidative stress and pro-inflammatory cytokines; regulating the expression of Bax, Bcl-2 and VEGF-A; and improving ovarian function and follicular reserve.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 5","pages":"Article 104324"},"PeriodicalIF":3.7,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141933650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enucleated egg donation: why is it important to understand the attitudes of women on donating enucleated eggs?","authors":"Rekha Pillai,&nbsp;Meenakshi Choudhary","doi":"10.1016/j.rbmo.2024.104323","DOIUrl":"10.1016/j.rbmo.2024.104323","url":null,"abstract":"<div><p>Enucleated egg donation is increasingly used for mitochondrial replacement therapy and in assisted conception to improve the success rate for women with recurrent IVF failure. With the possibility of a future increase in demand for enucleated egg donation, it is important to understand the attitudes of the general public and egg donors towards it. This Viewpoint elaborates on the general public's and donors’ perspectives on important aspects of enucleated egg donation that need to be explored, and points towards the areas that need to be researched in the future.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 3","pages":"Article 104323"},"PeriodicalIF":3.7,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141412632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why do oestrogens matter: systematic review and meta-analysis assessing GnRH antagonists, considering add-back therapy, for endometriosis-associated pain","authors":"","doi":"10.1016/j.rbmo.2024.104321","DOIUrl":"10.1016/j.rbmo.2024.104321","url":null,"abstract":"<div><p>Gonadotrophin-releasing hormone (GnRH) antagonists have been demonstrated to reduce endometriosis-associated pain. Because of the hypo-oestrogenic state they induce, however, higher dosages of GnRH antagonists are not recommended for used long term. This unwanted effect may be eliminated by so-called add-back therapy (ABT). This review was conducted to assess the safety and efficacy of GnRH antagonists, with or without add-back hormonal replacement therapy. Out of the 345 studies selected through the initial search, seven randomized controlled trials were included, comparing different oral GnRH antagonists at varying dosages, from a minimum of 50 mg to a maximum of 200 mg once or twice daily. Women treated with the lowest dose of GnRH antagonists had significantly greater mean pain score reductions from baseline throughout treatment compared with those treated with placebo (odds ratio [OR] –13.12, 95% CI –17.35 to –8.89 and OR –3.08, 95% CI –4.39 to –1.76 for dysmenorrhoea and non-menstrual pelvic pain, respectively). Compatible with the dose–response effect, a positive correlation was found between response rates and adverse event rates. While GnRH antagonists offer an advantage in terms of pain reduction for endometriosis, the more recent literature suggests using GnRH antagonists with ABT, which, while mitigating the hypo-oestrogenic effects of GnRH antagonists, maintain their efficacy, while allowing their long-term use.</p></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 4","pages":"Article 104321"},"PeriodicalIF":3.7,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472648324005108/pdfft?md5=0c7a0767404d6e55a97201706e8ed261&pid=1-s2.0-S1472648324005108-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141409155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}