Reproductive biomedicine online最新文献

{"title":"Telomere length of granulosa cells is positively associated with oocyte maturation and fertilization.","authors":"Shutian Jiang, Xueyi Jiang, Danjun Li, Qifeng Lyu, Wenzhi Li","doi":"10.1016/j.rbmo.2025.104803","DOIUrl":"https://doi.org/10.1016/j.rbmo.2025.104803","url":null,"abstract":"<p><strong>Research question: </strong>Is it feasible to use the telomere length of granulosa cells as a biomarker for ovarian function and embryological outcomes during IVF?</p><p><strong>Design: </strong>This prospective cohort study included 240 patients undergoing their first IVF cycle between October 2022 and December 2022. The main outcomes were the associations between relative telomere length of granulosa cells, collected during oocyte retrieval, and ovarian reserve, ovarian response and embryological outcomes.</p><p><strong>Results: </strong>The mean ± SD relative telomere length was -5.35 ± 2.55. No significant relationships were found between telomere length and ovarian reserve and ovarian response. Telomere length was positively correlated with maturation rate (r = 0.386, P < 0.001) and fertilization rate (retrieved oocytes: r = 0.408, P < 0.001; matured oocytes: r = 0.203, P = 0.002). However, telomere length was not significantly correlated with oocyte retrieval or viable embryo rate. On multifactor linear regression, relative telomere length was associated with oocyte maturation rate (P < 0.001) and fertilization rate of matured oocytes (P = 0.011). The receiver operating characteristic curve of telomere length as a predictor of oocyte maturity showed that the area under the curve (AUC) was 0.719 (P < 0.001), while the AUC of telomere length as a predictor of fertilization (of matured oocytes) was 0.613 (P = 0.005).</p><p><strong>Conclusion: </strong>Telomere length is correlated with embryological outcomes in IVF, mainly by affecting oocyte maturation and fertilization, rather than early embryo development. Telomere length alone cannot be used as a biomarker for ovarian reserve or ovarian response. When dealing with recurrent oocyte maturity or fertilization disorders, therapies oriented to lengthen telomeres or increase telomerase expression or function would facilitate cell division of granulosa cells, leading to higher oocyte maturation and fertilization rates.</p>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":" ","pages":"104803"},"PeriodicalIF":3.7,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Matter - Continued TOC","authors":"","doi":"10.1016/S1472-6483(24)00961-1","DOIUrl":"10.1016/S1472-6483(24)00961-1","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 1","pages":"Article 104772"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preconception carrier screening in couples seeking IVF: exploring the patient perspective","authors":"Celine Frank ,&nbsp;Claire H.C. Laeven ,&nbsp;Deidre Meulenbroeks ,&nbsp;Edith Coonen ,&nbsp;Christine E.M. de Die-Smulders ,&nbsp;Ronald J.T. van Golde ,&nbsp;Lonne M. Mallens ,&nbsp;Aimée D.C. Paulussen ,&nbsp;Juliette Schuurmans ,&nbsp;Liesbeth A.D.M. van Osch","doi":"10.1016/j.rbmo.2024.104452","DOIUrl":"10.1016/j.rbmo.2024.104452","url":null,"abstract":"<div><h3>Research question</h3><div>What is the level of understanding, and what are the attitudes and considerations regarding preconception carrier screening (PCS) among couples seeking IVF or intracytoplasmic sperm injection (ICSI)?</div></div><div><h3>Design</h3><div>A mixed-methods design was used. Nine interviews were conducted with couples or individual partners (<em>n</em> = 16) who had an initial consultation for IVF/ICSI in the 2 years preceding this study. A questionnaire was completed by 115 participants. No actual PCS was offered.</div></div><div><h3>Results</h3><div>All interviewed couples expressed a positive attitude towards PCS, and over half of the respondents stated that they would pursue or seriously consider pursuing PCS if possible. Some couples falsely believed that PCS could identify a cause for their fertility problems and increase their chance of conceiving. The desire to make an informed reproductive decision was the most important argument in favour of PCS. The primary argument against PCS was the apprehension of being confronted with reproductive dilemmas. The longer the delay to IVF/ICSI treatment required to perform PCS, the more couples would be inclined to decline screening. Participants indicated that they would prefer to receive information about PCS from a medical specialist at an early stage in their IVF/ICSI treatment.</div></div><div><h3>Conclusion</h3><div>Although attitudes towards PCS were generally positive, some concerns were raised about treatment delays and potential reproductive dilemmas, and some couples had misconceptions about the purpose of screening within the context of their IVF/ICSI treatment. These findings highlight the importance of tailoring information and counselling to the specific needs of couples seeking IVF/ICSI.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 1","pages":"Article 104452"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical outcomes of vitrified-warmed autologous oocyte cycles with 15-year follow-up at a single UK centre: consistent and predictable results","authors":"Jemma Garratt ,&nbsp;Trina Shah ,&nbsp;Anna Mclaughlin,&nbsp;Balsam Al-Hashimi,&nbsp;Nick Macklon,&nbsp;Elena Linara-Demakakou,&nbsp;Kamal K. Ahuja","doi":"10.1016/j.rbmo.2024.104376","DOIUrl":"10.1016/j.rbmo.2024.104376","url":null,"abstract":"<div><h3>Research question</h3><div>What were the clinical outcomes from 332 autologous vitrified- warmed oocyte cycles derived from 3182 elective autologous oocyte freeze cycles carried out between 2008 and 2022 in a single-centre series?</div></div><div><h3>Design</h3><div>In this retrospective observational study, outcomes in 299 patients returning to use their frozen oocytes between 2015 and 2023 were analysed.</div></div><div><h3>Results</h3><div>A total of 3328 elective oocyte vitrification cycles were performed in 2280 patients. The return rate to use oocytes was 14% (299/2171). Mean ages were 37.6 years at storage and 40 at warming. Ninety-three clinical pregnancies and 77 healthy live births were recorded. The live birth rate (LBR) was 24% (39/163) per fresh transfer and 17% (39/227) per embryo transferred. Stratified by age at freezing, the LBR per embryo transferred was 26% (12/47) in participants under 35 years, 20% (24/118) in those 35–39 years and 5% (3/62) in those 40+ years. Frozen embryo transfers (FET) achieved a 30% (24/80) LBR per embryo transfer and a 27% (24/90) LBR per embryo transferred. PGT-A for embryo selection doubled the LBR compared with FET from an untested embryo after one attempt (40% versus 21%). In patients aged over 40 years, the cumulative LBR reached 42% per patient in euploid FET.</div></div><div><h3>Conclusion</h3><div>The proportion of patients who returned to use their stored oocytes and the clinical outcomes were consistent with other recent reports and challenges the prevalent critical narrative regarding elective oocyte freezing for fertility preservation. The results are now comparable to routine IVF. Not everyone who returns to use their oocytes will conceive, but for those choosing to preserve their fertility, oocyte freezing can provide reproducible and reassuring results.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 1","pages":"Article 104376"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141724326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Editorial Board","authors":"","doi":"10.1016/S1472-6483(24)00963-5","DOIUrl":"10.1016/S1472-6483(24)00963-5","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 1","pages":"Article 104774"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polar body-based PGT-A: not dead yet? A step forward back to the roots of PGT-A","authors":"Anna Oberle,&nbsp;Michael Feichtinger","doi":"10.1016/j.rbmo.2024.104430","DOIUrl":"10.1016/j.rbmo.2024.104430","url":null,"abstract":"<div><div>Trophectoderm-based preimplantation genetic testing for aneuploidies (PGT-A) is used worldwide as a means of selecting embryos with high potential for achieving a live birth. However, trophectoderm analysis may be impaired through embryonic mosaicism, leading to genetically healthy embryos being falsely discarded, and thus even reducing cumulative live birth rates. Polar body biopsy, a technique applied since the early days of preimplantation testing, has been abandoned by most IVF centres. In comparison to trophectoderm analysis, however, polar body biopsy might even have certain advantages over trophectoderm PGT-A. This Countercurrent contribution discusses the newest clinical evidence, as well as ethical and cost-efficiency considerations, and argue that polar body analysis should be reconsidered.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 1","pages":"Article 104430"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Looking into the future: a machine learning powered prediction model for oocyte return rates after cryopreservation","authors":"Yuval Fouks ,&nbsp;Pietro Bortoletto ,&nbsp;Jeffrey Chang ,&nbsp;Alan Penzias ,&nbsp;Denis Vaughan ,&nbsp;Denny Sakkas","doi":"10.1016/j.rbmo.2024.104432","DOIUrl":"10.1016/j.rbmo.2024.104432","url":null,"abstract":"<div><h3>Research question</h3><div>Could a predictive model, using data from all US fertility clinics reporting to the Society for Assisted Reproductive Technology, estimate the likelihood of patients using their stored oocytes?</div></div><div><h3>Design</h3><div>Multiple learner algorithms, including penalized regressions, random forests, gradient boosting machine, linear discriminant analysis and bootstrap aggregating decision trees were used. Data were split into training and test datasets. Patient demographics, medical and fertility diagnoses, partner information and geographic locations were analysed.</div></div><div><h3>Results</h3><div>A total of 77,631 oocyte–cryopreservation cycles (2014–2020) were analysed. Patient age averaged 34.5 years. Treatment indications varied: planned (35.6%), gender-related (0.1%), medically indicated (15.5%), oncologic (5.7%) and unknown (42.3%). Infertility diagnoses were less common: unexplained infertility (1.8%), age-related infertility (3.2%), diminished ovarian reserve (9.9%) and endometriosis (1.6%). An ensemble model combining bootstrap aggregation classification and regression trees, stochastic gradient boosting and linear discriminant analysis yielded the highest predictive accuracy on test set (balanced accuracy: 0.83, sensitivity: 0.76, specificity: 0.91), with a receiver operating characteristic curve of 0.90 and precision-recall curve and area under the curve of 0.57. Key factors influencing the likelihood of returning for oocyte use included patient age, presence of a partner, race or ethnicity, the clinic's geographic region and oocyte cryopreservation indication.</div></div><div><h3>Conclusions</h3><div>This model demonstrated significant predictive accuracy, and is a valuable tool for patient counselling on oocyte cryopreservation. It helps to identify patients more likely to use stored oocytes, enhancing healthcare decision-making and the efficiency of gamete storage programmes. The model can be applied to self-financed and insurance-funded cycles.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 1","pages":"Article 104432"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The First Lugano Workshop on the role of adenomyosis in ART","authors":"Mauro Cozzolino ,&nbsp;Serdar Bulun ,&nbsp;Dominique De Ziegler ,&nbsp;Caterina Exacoustos ,&nbsp;Human Fatemi ,&nbsp;Juan Antonio Garcia-Velasco ,&nbsp;Andrew Horne ,&nbsp;Felice Petraglia ,&nbsp;Pietro Santulli ,&nbsp;Edgardo Somigliana ,&nbsp;Kim Soorin ,&nbsp;Thierry Van den Bosch ,&nbsp;Paola Viganò ,&nbsp;Peter Humaidan","doi":"10.1016/j.rbmo.2024.104444","DOIUrl":"10.1016/j.rbmo.2024.104444","url":null,"abstract":"<div><div>Adenomyosis is an important clinical condition with uncertain prevalence, and clinical focus on adenomyosis in patients undergoing assisted reproductive technology (ART) has increased during recent years. Recognizing the limited clinical knowledge on the impact of adenomyosis on ART outcomes, the First Lugano Adenomyosis Workshop was a symposium involving experts in the field of adenomyosis, covering basic research, imaging, surgery and infertility to highlight current advances and future research areas over a wide range of topics related to adenomyosis. Adenomyosis is characterized by altered oestrogen and progesterone signalling pathways. Although the criteria of the Morphological Uterus Sonographic Assessment (MUSA) Consortium apply to patients with infertility, the presence of direct signs and localization in the different myometrial layers, particularly the inner myometrium, need more focus. In addition to the MUSA criteria, clinical symptoms and the magnitude of uterine enlargement should also be considered. Whilst pre-treatment with gonadotrophin-releasing hormone agonist with or without an aromatase inhibitor in frozen embryo transfer cycles seems promising, many issues related to therapy remain unanswered. During the Workshop, therapeutic progress over the past decades as well as novel insights were presented and discussed. The role of this opinion paper is to stimulate discussion and spark further interest in adenomyosis and the role of adenomyosis in infertility.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 1","pages":"Article 104444"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cairo Consensus on Accreditation as the Basis for Future-Proofing the ART Laboratory","authors":"Cairo Consensus Group 2023,&nbsp;Alpha Scientists in Reproductive Medicine","doi":"10.1016/j.rbmo.2024.104106","DOIUrl":"10.1016/j.rbmo.2024.104106","url":null,"abstract":"<div><div>An international consensus meeting was convened to discuss globally applicable strategies for ‘future-proofing’ ART laboratories. The central theme was how the application of the foundational principles of laboratory accreditation enables any centre to create an ethos and framework that will support future-proofing in all regards. Discussions focussed on ART laboratory services from egg retrieval and semen specimen receipt to embryo transfer, as well as pertinent cryobanking activities. Issues related to whether ART treatment should be considered an essential service, overall clinic operations, general patient care, and the provision of clinical treatment, were not included as they fall under the purview of physicians and public health authorities. This report details the 16 core consensus points reached, which are supported by extensive practical recommendations that cover the gamut of ART laboratory operations.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 1","pages":"Article 104106"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141049137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of the shortened warming protocol on human blastocyst viability: an in-vitro experimental study","authors":"Kenji Ezoe,&nbsp;Tetsuya Miki,&nbsp;Nanoha Fujiwara,&nbsp;Keiichi Kato","doi":"10.1016/j.rbmo.2024.104454","DOIUrl":"10.1016/j.rbmo.2024.104454","url":null,"abstract":"<div><h3>Research question</h3><div>Does the shortened warming protocol impact the cell viability and outgrowth competence of human vitrified blastocysts warmed with or without fatty acids?</div></div><div><h3>Design</h3><div>In this study, 326 discarded vitrified human blastocysts donated for research by consenting couples were used. The blastocysts were randomly allocated to five groups depending on the warming solutions, protocols and recovery culture media: the control-conventional, control-shortened, FA-conventional, FA-shortened, and FA-shortened/recovery culture with fatty acid (FA-shortened/RF) groups. The blastocysts were warmed with or without fatty acids following the manufacturer's instruction (conventional method) or using the shortened method, in which blastocysts were immersed in a thawing solution for 1 min and then cultured in the recovery medium for 2 h. The embryo volume recovery, cell viability, intracytoplasmic lipid droplets and outgrowth competence were evaluated.</div></div><div><h3>Results</h3><div>The degree of blastocyst volume recovery was significantly higher after shortened warming than after conventional warming (<em>P</em> = 0.0130–0.0278). Cell membrane collapse was observed during the shortened warming. The blastocyst survival rate and expansion status after the recovery culture were comparable among the five groups. However, the proportion of necrotic cells was increased in the control-shortened, FA-shortened and FA-shortened/RF groups (<em>P</em> = 0.0160–0.0498). The adhesion rates were comparable among all the groups; however, the outgrowth area was significantly higher in the FA-conventional group than in the other groups at 96 h (<em>P</em> = 0.0026–0.0487).</div></div><div><h3>Conclusions</h3><div>The efficacy of the shortened warming protocol depends on the warming solutions used. The shortened protocol effectively improves the daily workflow; however, to prioritize clinical outcomes, each laboratory should thoroughly examine its impact before introducing it.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 1","pages":"Article 104454"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}