Reproductive biomedicine online最新文献

{"title":"UNRAVELING THE IMPACT OF BMAL1-MEDIATED CIRCADIAN RHYTHM DISRUPTION ON TROPHOBLAST INVASION AND MIGRATION THROUGH WNT/Β-CATENIN AND INTEGRINMEDIATED SIGNALING PATHWAYS","authors":"Begum Durkut , Melike Ucak , Saliha Surme , Ibrahim Halil Kavakli , Ciler Celik-Ozenci","doi":"10.1016/j.rbmo.2024.104557","DOIUrl":"10.1016/j.rbmo.2024.104557","url":null,"abstract":"<div><h3>Objective</h3><div>Circadian rhythm (CR) orchestrates various physiological processes on a 24-hour basis, governed by molecular clock genes (MCGs); <em>BMAL1, CLOCK, PER</em>s, and <em>CRY</em>s. Clinical data indicate that disruption of maternal CR during pregnancy is associated with intrauterine growth restriction (IUGR), a condition linked to defects in trophoblast migration and invasion, yet the underlying molecular mechanisms need to be explored. This study aims to elucidate rhythmic expression pattern of <em>BMAL1</em> in human villous trophoblast (HVT) and to investigate the specific pathways governed by <em>BMAL1</em> related to trophoblast migration and invasion.</div></div><div><h3>Materials and methods</h3><div>We utilized a pLenti-<em>BMAL1</em>-DLuc lentivirus for <em>in vitro</em> circadian rhythm monitoring of <em>BMAL1</em> gene. <em>BMAL1</em> expression was analyzed using JTK-Cycle, robust non-parametric algorithm for detecting rhythmic elements in genome-scale data. Non-target, and <em>BMAL1</em>-silenced groups were established using HVT cells. <em>BMAL1</em>-silenced group was created using shRNA-silencing strategy. Following transduction and subsequent selection, cells were harvested every six hours over 48-hour period, corresponding to Zeitgeber Time (ZT) points 0- 48, following dexamethasone treatment used for cell-cycle synchronization. <em>CLOCK, PER</em>s, <em>CRY</em>s, <em>RORα</em>, and <em>NR1D1</em> expression were quantified every 6 hours via qPCR. Trophoblast migration/invasion, influenced by BMAL1, were assessed through transwell assay, and related protein expressions (β-catenin, integrin-β1, integrin-ɑ5) were evaluated using western blotting. Statistical significance was determined at p<0.05 using two-way ANOVA and Tukey's post hoc test.</div></div><div><h3>Results</h3><div>The outcomes from JTK-Cycle analysis, revealed the inherent rhythmic expression pattern of <em>BMAL1</em> in HVT cells. This natural expression displayed an average period of 23.3 hours and an average amplitude of 429,7 abs. BMAL1 silencing was confirmed at the protein level. Rhythmic expression of other MCGs; <em>CLOCK, PER1/2/3, CRY1/2, RORα</em>, and <em>NR1D1</em> genes were altered significantly in <em>BMAL1</em>-silenced group(p<0.05). Following <em>BMAL1</em> silencing, a notable reduction in the migration and invasion capabilities of HVT cells was observed(p<0.05). Additionally, <em>BMAL1</em> depletion correlated with a significant decrease in β-catenin expression from ZT30 onwards, and a decrease in integrin-β1 expression from ZT36 onwards(p<0.05). Conversely, there was a significant increase in integrin-ɑ5 protein expression beginning at ZT24(p<0.05).</div></div><div><h3>Conclusions</h3><div><em>BMAL1</em> is rhythmically expressed in HVT cells, and its silencing disrupts rhythmic expression of key MCGs and leads to alterations in trophoblast migration and invasion-related pathways. Specifically, <em>BMAL1<","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104557"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECT OF ACETYL L-CARNITINE ADMINISTRATION ON TESTICULAR HISTOPATHOLOGY, APOPTOSIS, AND OXIDATIVE STRESS IN AN EXPERIMENTAL CHRONIC ALCOHOLISM MODEL","authors":"Merve Gorgulu ,&nbsp;Gizem Gamze Tas ,&nbsp;Gamze Zengin ,&nbsp;Hakan Er ,&nbsp;Sevim Ercan Kelek ,&nbsp;Leyla Sati","doi":"10.1016/j.rbmo.2024.104540","DOIUrl":"10.1016/j.rbmo.2024.104540","url":null,"abstract":"<div><h3>Objective</h3><div>Alcohol addiction is recognized as a serious public health issue today. The World Health Organization classifies alcohol as substances that cause both physiological and psychological dependence when consumed over prolonged periods. Studies clearly indicate that chronic alcohol consumption has detrimental effects on the male reproductive system, suggesting that it is a risk factor for male fertility with reproductive and endocrine problems. Acetaldehyde, which is formed by the metabolism of alcohol, causes the production of free radicals when oxidized, which triggers apoptotic processes and oxidative stress. It is known that chronic alcohol use results in oxidative stress. However, various studies have shown that acetyl-L-carnitine (ALCAR) has antioxidant effects on several biological mechanisms under different conditions. Therefore, we aimed to investigate the potential effects of ALCAR administration on sperm motility, testicular histopathology, apoptosis, and oxidative stress in rats chronically exposed to ethanol (EtOH).</div></div><div><h3>Materials and Methods</h3><div>Rats were randomly separated into 4 groups. Over the course of the 4-week period, EtOH (5 g/kg, 25% w/v), ALCAR (50 mg/kg), and EtOH (5 g/kg, 25% w/v) + ALCAR (50 mg/kg) were given to rats by oral gavage daily (n=10/group). However, the control group received only distilled water. Epididymal sperm motility and testicular weight index were measured in line with testicular histopathology and modified Johnsen scoring. TUNEL assay was used for the assessment of the apoptotic index. Total oxidants and antioxidant status were analyzed with ELISA.</div></div><div><h3>Results</h3><div>Sperm motility and testicular modified Johnsen scores were decreased in the EtOH group (p&lt;0.001). However, the EtOH+ALCAR group showed similar levels as to the control group. Even though no significant difference was determined for testicular weight index, immature germ cells spilled into the lumen, vacuolization in the intertubular area, and expansion in the interstitial area were observed in the EtOH group in contrast to normal testicular histology in the EtOH+ALCAR group. Chronic alcohol administration caused a significant increase in the apoptotic index (p&lt;0.05) and oxidative stress (p&lt;0.001). Nevertheless, we detected decreased apoptotic index and oxidative stress levels in the EtOH+ALCAR group compared to the EtOH group.</div></div><div><h3>Conclusion</h3><div>Our results indicate that ALCAR, an antioxidant compound, may have beneficial effects against the damages caused by chronic use of alcohol, such as poor sperm motility, testicular atrophy, and increased oxidative stress. However, the exact efficacy of ALCAR on the treatment for male infertility and metabolic consequences of chronic alcohol consumption needs to be confirmed by further investigations.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104540"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MENOPAUSAL SYMPTOMS AND SYSTEMIC INFLAMMATION: A COMPARATIVE STUDY IN PERIMENOPAUSAL AND POSTMENOPAUSAL WOMEN","authors":"Busra Korpe ,&nbsp;Caner Kose","doi":"10.1016/j.rbmo.2024.104561","DOIUrl":"10.1016/j.rbmo.2024.104561","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate menopausal symptoms and their frequencies while exploring their potential correlation with systemic inflammation in perimenopausal and postmenopausal women.</div></div><div><h3>Materials and Methods</h3><div>The study involved a comparative analysis of menopausal symptoms and systemic inflammation markers between perimenopausal (n=213) and postmenopausal women (n=219). Participants were assessed with the Menopause Rating Scale (MRS) for somatic, urogenital and psychological symptoms and systemic immune-inflammation index (SII) levels. Regression analysis was conducted to identify parameters strongly associated with high somatic and total MRS scores.</div></div><div><h3>Results</h3><div>Total MRS and somatic domain scores were found to be significantly higher in perimenopausal women compared to postmenopausal women. Additionally, perimenopausal women exhibited elevated levels of systemic inflammation, as indicated by higher SII levels. Regression analysis revealed that being perimenopausal, being smoker and having higher SII results were the most significant parameters related to increased somatic scores. In contrast, parity and exercise were found to be associated with lower somatic scores.</div></div><div><h3>Conclusion</h3><div>The findings of this study highlight the heightened somatic symptomatology and systemic inflammation observed in perimenopausal women. Specifically, being in the perimenopausal phase, being a smoker and having elevated systemic inflammation markers were identified as strong factors associated with increased somatic scores. These results underscore the importance of considering systemic inflammation in the management of menopausal symptoms, particularly during the perimenopausal transition.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104561"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IS PROGESTERONE SUPPLEMENTATION REQUIRED FOR LUTEAL PHASE SUPPORT DURING MODIFIED NATURAL CYCLE FROZEN EMBRYO TRANSFER?","authors":"Gönül Özer ,&nbsp;Sevinc Özmen","doi":"10.1016/j.rbmo.2024.104568","DOIUrl":"10.1016/j.rbmo.2024.104568","url":null,"abstract":"<div><h3>Objective</h3><div>Is there a difference in pregnancy outcomes among women undergoing modified natural cycle frozen embryo transfer (mNC-FET) with or without progesterone as luteal phase support (LPS)?</div></div><div><h3>Materials and Methods</h3><div>A retrospective analysis was conducted on 2216 modified natural cycle frozen embryo transfer (mNC-FET) cycles performed at Memorial Sisli Hospital between 2011 and 2023. The study included patients under 38 years of age who underwent top-quality (TQ) or good-quality (GQ) single embryo transfer in mNC-FET cycles. Excluded from the study were medium-quality (MQ) or poor-quality (PQ) embryo transfer cycles, double embryo transfer cycles, and embryo transfer cycles with preimplantation genetic testing for aneuploidy (PGD/A). During the mNC-FET cycles, patients were monitored using transvaginal ultrasonography starting from the second day of the menstrual cycle to determine the day of ovulation. Based on follicle size and hormone levels, ovulation was induced with a single subcutaneous dose of recombinant human chorionic gonadotropin (r-hCG, Ovitrelle 250 µg). The cycles were divided into three separate groups for analysis: Group A (n=493) did not receive any luteal phase support; Group B (n=1327) received 200 mg of vaginal micronized progesterone twice daily; and Group C (n=396) received 200 mg of vaginal micronized progesterone twice daily along with 25 mg of subcutaneous progestin daily. Groups were compared in terms of cycle characteristics and pregnancy outcomes.</div></div><div><h3>Results</h3><div>Demographic and fresh cycle characteristics of the groups were similar. There was no statistically significant difference between the groups regarding pregnancy outcomes. Live birth rates were 58.4% in Group A, 60.8% in Group B and 60.1% in Group C (p=0.650). Clinical pregnancy rates were 65.9%, 69.1% and 68.2%, respectively (p=0.432). Biochemical abortion rates were 4.5%, 6.6% and 5.3%, respectively (p=0.186) and clinical abortion rates were 6.3%, 6.7% and 5.3%, respectively (p=0.828).</div></div><div><h3>Conclusion</h3><div>The study showed that women under 38 years of age undergoing mNC FET had similar pregnancy outcomes with or without the use of progesterone for luteal phase support. Consequently, the administration of progesterone for luteal phase support seems unnecessary. In these cases, hCG given for triggering and the presence of corpus luteum are sufficient for luteal phase support. Randomised controlled trials are needed to support these findings.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104568"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AGE-STRATIFIED ANALYSIS OF ANTI-MüLLERIAN HORMONE (AMH) LEVELS AND THE PREVALENCE OF DIMINISHED OVARIAN RESERVE IN A POPULATION OF 22,920 WOMEN AGED 18-45: A RETROSPECTIVE STUDY","authors":"Kiper Aslan ,&nbsp;Aylin Tunali ,&nbsp;İlayda Tellioğlu ,&nbsp;Isil Kasapoglu ,&nbsp;Gurkan Uncu","doi":"10.1016/j.rbmo.2024.104550","DOIUrl":"10.1016/j.rbmo.2024.104550","url":null,"abstract":"<div><h3>Objective</h3><div>Anti-Müllerian hormone (AMH) is recognized as the most reliable indicator of ovarian reserve and is widely utilized in various clinical settings. AMH concentrations consistently decline as women age. Despite its widespread use, the lack of standardized, age-specific reference ranges for AMH in reproductive-aged women restricts its full clinical utility. This retrospective analysis aims to explore age-stratified AMH levels and establish the prevalence of diminished ovarian reserve (DOR) at different ages.</div></div><div><h3>Methods</h3><div>The study was conducted at a tertiary university hospital. An electronic database was screened for AMH results between the years 2015-2024. All analyzed AMH results were recorded regardless of the purpose of the analysis. Women aged between 18-45 were enrolled, ensuring each patient was only included once to avoid duplications. Correlation analysis was performed to show the relationship between AMH and age. Median AMH values for each age group were calculated, and the prevalence of diminished ovarian reserve according to AMH levels was analyzed. The Poseidon Criteria were used to define diminished ovarian reserve (&lt;1.2 ng/ml).</div></div><div><h3>Results</h3><div>A total of 24,587 AMH results were found in the electronic database for the years 2015-2024. After excluding duplications and results for women older than 45 and younger than 18, 22,920 AMH results were analyzed. Correlation analysis showed a significant relationship between AMH levels and age. As age increased, AMH levels significantly decreased. By age 36, median AMH levels had decreased below 1.2 ng/ml. The prevalence of DOR was 15.9% at age 18, while this ratio was nearly 96% at age 45.</div></div><div><h3>Conclusion</h3><div>This study presented age-stratified AMH levels for a large population of over 20,000 women. Although the study population consisted of patients from a tertiary hospital, the results may be beneficial in establishing age-specific AMH levels in clinical practice.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104550"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VAGINAL MICROBIOME TRANSPLANTATIONS - A POTENTIAL NEW TREATMENT IN REPRODUCTIVE DISEASE","authors":"Henriette Svarre Nielsen","doi":"10.1016/j.rbmo.2024.104508","DOIUrl":"10.1016/j.rbmo.2024.104508","url":null,"abstract":"<div><div>This talk is about the vaginal microbiome and will present studies about vaginal and endometrial dysbiosis and reproductive outcomes. Treatment studies will be presented including the Dyscover studies on vaginal microbiome transplantation.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104508"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF THE RELATIONSHIP BETWEEN OBESITY AND OXIDATIVE STRESS IN PATIENTS WITH ADOLESCENT POLYCYSTIC OVARY SYNDROME","authors":"Cahidenur Selveroğlu ,&nbsp;Banuhan Şahin ,&nbsp;Hanife Kara","doi":"10.1016/j.rbmo.2024.104554","DOIUrl":"10.1016/j.rbmo.2024.104554","url":null,"abstract":"<div><h3>Objective</h3><div>Polycystic ovary syndrome (PCOS) is one of the most common diseases that starts in adolescence and is common in women of reproductive age. PCOS patients have menstrual dysfunction, signs of hyperandrogenism (such as hirsutism, acne), metabolic problems, increased cardiovascular risk, insulin resistance, risk of developing type 2 diabetes mellitus. Oxidative stress; It has high levels in patients with central obesity, insulin resistance and hyperandrogenicity. Human and animal studies on oxidative stress in insulin resistance in Turkey and in the world are insufficient. Based on this information, we aimed to obtain new data on the relationship between oxidative stress parameters, total antioxidant level (TAS), total oxidant level (TOS), and oxidative stress index (OSI) levels, with obesity in adolescence with PCOS.</div></div><div><h3>Materials and Methods</h3><div>Between 01.02.2021 and 01.02.2022, Amasya University Sabuncuoğlu Şerefeddin Education and Research Hospital Preferred to Gynecology and Obstetrics outpatient clinics, four groups were created, 31 normal weight and 32 overweight/obese non-PCOS; 30 normal weight and 32 overweight/obese PCOS. Rotterdam criteria was used for PCOS diagnosis. Research permission and informed consent were obtained from all patients. Blood samples were analyzed for glucose, total testosterone (TT), dehydroepiandrosterone sulfate (DHEA-S), Luteinizing hormone (LH), Follicle stimulant hormone (FSH), estrogen, progesterone, 17OH-progesterone (17OH-P), creatinine and hemogram levels. TAS, TOS and OSI were calculated with TAS and TOS kits and differences between groups were examined.</div></div><div><h3>Result</h3><div>Among the study groups, TT, 17OH-P, DHEA-S, WBC, CRP, ALT values, TAS, TOS and OSI were significantly higher in the obese and PCOS groups. While there is a strong correlation between TOS and OSI; there is a moderate degree between TAS and OSI. As part of the research, especially in the Obese/PCOS group patients, as the WBC value increased compared to other groups, TOS increased; TAS increased as CRP value increased; TOS increased as TT increased; As the ALT value increased, it was found that both TAS and TOS increased. A statistically significant difference in TOS and TAS values was found in the Obese/PCOS groups with Normal Weight/Normal Patient. A statistically significant difference in TAS values was found in the Obese/PCOS binary group comparison with Normal Weight/PCOS.</div></div><div><h3>Conclusion</h3><div>In our study, we observed changes in TOS and OSI levels in overweight/obese and non-overweight/obese adolescences with PCOS and non-PCOS. An inflammatory process in obesity was found to be effective in pathology of adolescence PCOS.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104554"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COMPARISON OF CYCLE CHARACTERISTICS OF PATIENTS WITH UNEXPLAINED INFERTILITY TREATED WITH PROGESTIN PRIMED OVARIAN STIMULATION AND ANTAGONIST PROTOCOL","authors":"Ramazan Erda Pay ,&nbsp;Oya Aldemir ,&nbsp;Runa Özelçi ,&nbsp;Candan İltemir Duvan ,&nbsp;Serdar Dilbaz ,&nbsp;Yaprak Üstün","doi":"10.1016/j.rbmo.2024.104566","DOIUrl":"10.1016/j.rbmo.2024.104566","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;div&gt;The aim of controlled ovarian hyperstimulation (COH) in assisted reproductive techniques is to obtain the maximum number of mature oocytes required to achieve pregnancy. At this stage, where follicular development is simultaneously achieved, it is very important to prevent premature ovulation that may develop before the oocyte retrieval procedure and premature luteinizing hormone (LH) peak.(1) Gonadotropin-releasing hormone (GnRH) agonists and antagonists have been used for many years to prevent premature LH peak by suppressing the pituitary gland. Although GnRH agonists or antagonists provide satisfactory results in this regard, they create disadvantages for patients due to the discomfort caused by their daily injection and their high prices. Progestins, in addition to effectively suppressing the LH peak and ovulation, are also cheap and easy to use, which has led to numerous studies in recent years evaluating the effects of different progesterone preparations on different patient groups, and different progestin-primed ovarian stimulation (PPOS) protocols have been investigated.(2) In our study, we aimed to retrospectively compare the COH results obtained in patients who applied to our clinic with the indication of unexplained infertility and who were applied PPOS or antagonist protocols.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Materials and Methods&lt;/h3&gt;&lt;div&gt;For our cross-sectional retrospective observational study, 138 patient data from the Health Sciences University Etlik Zübeyde Hanım Gynecology and Pediatrics Training and Research Hospital UYT/IVF unit, whose diagnosis, follow-up and treatments were planned, and whose COH results were obtained, between January 2022 and March 2023, were retrospectively scanned from the patient registration system. For COH purposes, 59 patients (42.1%) who were applied PPOS protocol and 81 patients (57.9%) who were applied antagonist protocol were divided into group1 and group2. Demographic, laboratory, stimulation data and oocyte characteristics of the groups were compared.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Data of 59 patients (42.1%) who were applied PPOS protocol were compared in group1 and 81 patients (57.9%) who were applied antagonist protocol were compared in group2. No difference was observed between demographic data of the patients such as age, male age, infertility duration and BMI (p&gt;0.05). (Table1) No difference was observed between basal FSH, LH, E2, AFC and AMH values of the patients in terms of ultrasonography and laboratory values ​​(p&gt;0.05). (Table1) No difference was observed between clinical and COH results of the patients in terms of total gonadotropin dose, stimulation duration, number of oocytes collected and number of mature oocytes (p&gt;0.05). (Table1)&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;In our study, similar results were observed between the PPOS protocol and the antagonist protocol. PPOS can be seen as a good alternative to antagonist protocols due to the advant","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104566"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF ENDOPLASMIC RETICULUM STRESS IN GRANULOSA CELLS OF INFERTILE PATIENTS DIAGNOSED WITH ENDOMETRIOMA AND INVESTIGATION OF ITS EFFECT ON OOCYTE QUALITY","authors":"KÜBRA ÖZLEM BİLGİÇ ,&nbsp;IŞIL KASAPOĞLU ,&nbsp;MÜNİR KİPER ASLAN ,&nbsp;CİHAN ÇAKIR ,&nbsp;BERRİN AVCI ,&nbsp;GURKAN UNCU","doi":"10.1016/j.rbmo.2024.104547","DOIUrl":"10.1016/j.rbmo.2024.104547","url":null,"abstract":"<div><h3>Objective</h3><div>Endometriosis is a disease characterized by the implantation and growth of endometrial tissue outside the uterus in women of reproductive age, and its etiopathogenesis remains unclear. It is most commonly seen in the ovaries (endometrioma). It is estimated that it negatively affects folliculogenesis, ovulation, embryogenesis and causes infertility by causing endoplasmic reticulum (ER) stress in the ovary through different mechanisms. ER stress occurs when cell homeostasis is disrupted as a result of the accumulation of unfolded or misfolded proteins. In this case, the cell first activates ER stress response pathways, aiming to restore homeostasis and keep the cell alive. If ER stress cannot be coped with, the cell is directed to apoptosis. The aim in our study is to determine the local and/or systemic negative effects of endometriosis on the ovary, the endoplasmic reticulum stress it creates in the ovaries as a result of these effects, and which pathway or pathways are activated in case of stress.</div></div><div><h3>Materials and methods</h3><div>ER stress parameters (GADD34, Caspase12, Caspase3, BIP, CHOP, XBP1, ATF4) were investigated using ELISA method in granulosa cells, which are waste cells collected during oocyte retrieval after in vitro fertilization (IVF) treatment from infertile patients diagnosed with unilateral endometrioma and healthy female patients diagnosed with male factor and will not be used in IVF treatment. The study design was planned in 3 groups; Group 1: ovary with endometrioma diagnosed with unilateral endometrioma (n=23), Group 2: ovary without endometrioma diagnosed with unilateral endometrioma (n=23), Group 3: control group male factor (n=20).</div></div><div><h3>Conclusions</h3><div>Although no significant difference was observed in ovarian and embryological parameters between the two groups with and without endometrioma, a significantly lower total antral follicle count, AMH levels, oocyte retrieval numbers, oocyte maturation, and embryo quality were detected when compared to the control group. As the size of the endometrioma increased, particularly when exceeding a threshold of 4 cm, it was found that the PERK pathway, an ER stress pathway, as well as the caspase apoptotic process, were activated. We can say that the PERK pathway is primarily preferred among the ER stress pathways examined in the endometrioma group and by dimensioning. The results can be supported by more comprehensive studies.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104547"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A SINGLE-CENTER EXPERIENCE OF OVARIAN TISSUE TRANSPLANTATION IN 15 PATIENTS","authors":"Batuhan Aslan ,&nbsp;Nilüfer Akgün ,&nbsp;Meltem Sönmezer ,&nbsp;Yavuz Emre Şükür ,&nbsp;Sinan Özkavukçu ,&nbsp;Özgür Çınar ,&nbsp;Batuhan Özmen ,&nbsp;Cem Atabekoglu ,&nbsp;Murat Sönmezler","doi":"10.1016/j.rbmo.2024.104523","DOIUrl":"10.1016/j.rbmo.2024.104523","url":null,"abstract":"<div><h3>Objective</h3><div>Ovarian tissue cryopreservation (OTC) and transplantation has become a promising option for fertility preservation in women facing the risk of premature ovarian failure. We aim to report our experience with 15 patients who underwent frozen thawed ovarian tissue transplantation (OTT).</div></div><div><h3>Methods</h3><div>This retrospective cohort study included 15 women who underwent OTT in our tertiary referral center between December 2011 and June 2024. All the patients had previously undergone OTC using a slow freezing protocol with a home-made pre-prepared freezing medium including DMSO and sucrose. All patients diagnosed with acute leukemia had received consolidation chemotherapy prior to OTC before undergoing bone marrow transplantation (BMT). A medical clearance was obtained from each patient's hematologist-oncologist, and a vial of tissue was thawed to screen for the presence of residual leukemic cells. All OTT procedures were performed laparoscopically, with the ovarian cortical fragments being transplanted either into a retroperitoneal pocket (n=11), created 2 days earlier to enhance vascularization, or directly onto the menopausal ovary (n=1), or both(3).</div></div><div><h3>Results</h3><div>All 15 patients who underwent OTT regained ovarian function, as demonstrated by decreased FSH levels, increased estradiol and resumption of menstruation. Patient characteristics are shown in Table 1. No major surgical complications or adverse events were observed. The median age at OTC and OTT were 23.31± 7.9 years and 32.6 ± 1.48 years respectively. The indications were as follows; leukemia (n= 6), lymphoma (n=5), breast cancer (n=1), pineal gland tumor (n=1), thalassemia (n=1), and aplastic anemia (n=1). Mean serum AMH before OTC and following OTT was 1.37± and 0.16±0.11, respectively. Following IVF, 8 healthy live births occurred in 6 patients (Two of them was twin), summing up a pregnancy rate of 45.46% per patient (5/11) in those undergoing IVF (Patient 15 does not included). In one patient diagnosed with acute leukemia, IVF resulted in a healthy ongoing pregnancy following second OTT, and in another patient diagnosed with acute leukemia 2 euploid blastocysts were frozen in the first ovarian stimulation cycle (patient 14). In patient 15, who had 2 previous IVF cancellation due to POR, a healthy live birth occurred from the menopausal ovary following retroperitoneal OTT. Except one patient who underwent OTT very recently, no relapse was recorded in 5 patients with acute leukemia over a mean follow-up &gt;50 months. Graft survival was &gt; 24 months in all patients.</div></div><div><h3>Conclusion</h3><div>Transplantation of frozen thawed ovarian tissue remains a promising and established technique for fertility preservation, with prolonged graft longevity and increased pregnancy rates. We suggest that OTT may be feasible in carefully screened leukemia survivors following BMT.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104523"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}