Reproductive biomedicine online最新文献

{"title":"BLASTOCYST RE-EXPANSION ONE HOUR POST-BIOPSY: AN INDEPENDENT PREDICTOR OF LIVE BIRTH","authors":"Ibrahim Elkhatib","doi":"10.1016/j.rbmo.2024.104506","DOIUrl":"10.1016/j.rbmo.2024.104506","url":null,"abstract":"<div><div>Blastocyst cryopreservation is a key component of PGT-A procedures. In non-PGT-A cycles, collapsing blastocysts before vitrification is commonly recommended to improve blastocyst viability. However, in PGT-A cycles insufficient information is provided on the matter. The European Society of Human Reproduction and Embryology recommends an immediate freeze post-biopsy, although a definitive timeframe remains elusive. Some studies support prolonged culture post-biopsy (&gt; 3 hours), citing improved implantation and pregnancy rates. Conversely, others advocate for rapid cryopreservation, endorsing an immediate approach within an hour, while most blastocysts are still collapsed due to the biopsy procedure. To date, no conclusive timing has been agreed upon. We retrospectively studied 1141 single euploid blastocyst transfers (seFET) conducted between April 2021 and February 2023. Blastocysts were scored at 1-hour post-trophectoderm (TE) biopsy as follows: 0 for complete collapse, 1 for starting re-expansion, and 2 for clear re-expanded cavity.</div><div>A total of 569 seFET resulted in live birth (49.8%), 387 (33.9%) did not lead to pregnancy, 76 (6.7%) resulted in biochemical miscarriage, and 107 (9.4%) resulted in clinical miscarriage. One-hour post-biopsy, 64.0% of blastocysts re-expanded to Grade 2, 29.3% to Grade 1, and 6.7% showed no re-expansion (Grade 0). LB rate was 54.2%, 44.3% and 32.5% (P&lt;0.001) among embryos that re-expanded to Grade 2, 1 or 0, respectively. Regression analysis showed re-expanding to Grade 2 at 1-hour post-biopsy was significantly and independently associated with LB compared to embryos that did not re-expand (i.e., Grade 0) (aOR: 1.77, 95% CI: 1.01–3.12), after adjusting for embryo quality (inner cell mass (ICM), TE grade, day of biopsy), BMI and endometrial preparation protocol. Embryos with greater re-expansion 1-hour post-biopsy had higher re-expansion slopes post-warming, indicating faster and greater re-expansion to the point of transfer. Differences were significant for all trophectoderm categories (Grade A, B, and C; P=0.016, &lt;0.001 and &lt;0.001, respectively).</div><div>Embryo re-expansion 1-hour post-biopsy was associated with post-warming re-expansion and increased LB rates.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104506"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NON-INVASIVE PGTA by AI","authors":"Dr. Marcos Meseguer","doi":"10.1016/j.rbmo.2024.104515","DOIUrl":"10.1016/j.rbmo.2024.104515","url":null,"abstract":"<div><div>The application of artificial intelligence (AI) in assisted reproduction addresses the complex landscape of infertility, a prevalent condition affecting a significant percentage of the reproductive-age population. Advances in reproductive medicine, marked by milestones such as in vitro fertilization (IVF) and intracytoplasmic sperm microinjection (ICSI), have led to the development of assisted reproduction techniques (ART). While multiple embryo transfer (MET) has traditionally been employed to increase pregnancy chances, it carries risks. Therefore, embryo selection techniques have suffered a rapid increase in interest. The introduction of incubators with time-lapse technology allowed embryo analysis without disturbing culture conditions and involved the introduction of the first embryo selection algorithms. Consequently, developing and including AI approaches is the current challenge. This presentation addresses real-world challenges in the embryology field by applying deep learning methods. The final goal is to design, develop, and validate tools that support the daily routine in an IVF laboratory, ultimately improving success rates in assisted reproductive clinics. The complexity of the solved tasks increases systematically, providing consistent knowledge based on embryology. Specific goals involve solving concrete tasks with different methodologies and exploring novel AI techniques. The tasks include fecundation, viability, quality, and prediction of euploid embryos. The technical approaches encompass automation, segmentation, supervised contrastive learning, and inductive transfer techniques. The findings contribute to the field of embryology, showcasing potential applications of innovative AI methodologies. The incorporation of AI into the sperm selection process is one of the most explored areas in recent years within the field of assisted reproduction too and several AI-tools have been developed for analyzing oocyte quality according to its morphology. Ovarian stimulation is also one of the directions in the landscape of assisted reproduction which can be improved with the help of AI and there are studies in this direction using AI. Future goals introduce consistent integration into consultation and embryology laboratories, taking into account real clinical conditions, contributing to improved success rates in assisted reproduction clinics, and further exploring non-invasive techniques for genetic analysis.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104515"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COMPARISON OF PREGNANCY ACHIEVEMENT IN DAY 7 EMBRYOS WITH NGS DEPENDING ON AGE","authors":"Duygu Kütük ,&nbsp;İbrahim Orçun Olcay ,&nbsp;Berkay Akçay ,&nbsp;Sinan Özkavukçu","doi":"10.1016/j.rbmo.2024.104571","DOIUrl":"10.1016/j.rbmo.2024.104571","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;div&gt;After the fertilization stage, extending embryo culture (blastocyst culture) until day 5 may be the most appropriate and alternative way to select embryos for transfer. Extending embryo developmental follow-up until day 5/6 is a selection tool to select embryos with a higher probability of implantation rather than improving embryo quality. The developmental potential of D5, D6 and D7 blastocysts gradually decreases with increasing culture time. Therefore, the traditional practice in the laboratory is to select embryos for transfer, biopsy or cryopreservation at D5/D6. Studies on D7 blastocysts are very limited and controversial. Clinically, in some patients, if blastocysts cannot form at D5/D6 due to poor embryo quality due to advanced maternal age or other reasons, they can be cultured until D7. Therefore, the use of slowly developing D7 blastocysts will be of great importance for these patients.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Materials and methods&lt;/h3&gt;&lt;div&gt;This is a retrospective study planned to monitor paternal and maternal age, preimplantation genetic diagnosis/screening results and pregnancy status of patients who came for IVF treatment during the 7th day embryo development process.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;84 embryos of 73 patients who came to Bahçeci Umut IVF Center and completed their development on the 7th day and were frozen by NGS were included in the study. NGS results of 14 patients were normal, NGS results of 54 patients were abnormal, DNA was not detected in 2 patients, and biopsy was performed on 2 patients but was not performed upon the patient's request. Of the 15 female patients who were &lt;35; 8 out of 17 embryos were abnormal (%47,05), 7 were normal (%41,17) and 2 embryos were biopsied for genetic processing but were not studied (%11,76). The results of 7 patients were abnormal (%46,7), the results of 6 patients were normal (%40) and 2 patients did not have biopsies taken for NGS and the material was not studied (%13,3). Of the 6 patients, 2 patients whose genetic screening results were normal had negative pregnancy results, but 2 patients were found to have pregnancies. 2 patients did not transfer their embryos despite the results. Of the 58 female patients who were &gt;35; 53 embryos out of 67 embryos of 58 patients were abnormal (%79,1), 12 embryos were normal (%17,9) and DNA was not detected in 2 embryos (%2,98). The results of 9 patients were normal (% 15,5), 47 patients were abnormal (% 81) and DNA was not detected in 2 patients (%3,44). Of the 5 patients with normal NGS results (%55,5), 2 had live births (% 40) and 2 had miscarriages (% 40). In the 4 patients with normal NGS results, pregnancy did not occur after embryo transfer (%45).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;In slowly developing embryos, when developmental follow-up is performed until Day 7, embryo transfer should definitely be planned with NGS. In older women (&gt;35 years old), if the embryo has completed its developme","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104571"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UNRAVELING THE IMPACT OF BMAL1-MEDIATED CIRCADIAN RHYTHM DISRUPTION ON TROPHOBLAST INVASION AND MIGRATION THROUGH WNT/Β-CATENIN AND INTEGRINMEDIATED SIGNALING PATHWAYS","authors":"Begum Durkut ,&nbsp;Melike Ucak ,&nbsp;Saliha Surme ,&nbsp;Ibrahim Halil Kavakli ,&nbsp;Ciler Celik-Ozenci","doi":"10.1016/j.rbmo.2024.104557","DOIUrl":"10.1016/j.rbmo.2024.104557","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;div&gt;Circadian rhythm (CR) orchestrates various physiological processes on a 24-hour basis, governed by molecular clock genes (MCGs); &lt;em&gt;BMAL1, CLOCK, PER&lt;/em&gt;s, and &lt;em&gt;CRY&lt;/em&gt;s. Clinical data indicate that disruption of maternal CR during pregnancy is associated with intrauterine growth restriction (IUGR), a condition linked to defects in trophoblast migration and invasion, yet the underlying molecular mechanisms need to be explored. This study aims to elucidate rhythmic expression pattern of &lt;em&gt;BMAL1&lt;/em&gt; in human villous trophoblast (HVT) and to investigate the specific pathways governed by &lt;em&gt;BMAL1&lt;/em&gt; related to trophoblast migration and invasion.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Materials and methods&lt;/h3&gt;&lt;div&gt;We utilized a pLenti-&lt;em&gt;BMAL1&lt;/em&gt;-DLuc lentivirus for &lt;em&gt;in vitro&lt;/em&gt; circadian rhythm monitoring of &lt;em&gt;BMAL1&lt;/em&gt; gene. &lt;em&gt;BMAL1&lt;/em&gt; expression was analyzed using JTK-Cycle, robust non-parametric algorithm for detecting rhythmic elements in genome-scale data. Non-target, and &lt;em&gt;BMAL1&lt;/em&gt;-silenced groups were established using HVT cells. &lt;em&gt;BMAL1&lt;/em&gt;-silenced group was created using shRNA-silencing strategy. Following transduction and subsequent selection, cells were harvested every six hours over 48-hour period, corresponding to Zeitgeber Time (ZT) points 0- 48, following dexamethasone treatment used for cell-cycle synchronization. &lt;em&gt;CLOCK, PER&lt;/em&gt;s, &lt;em&gt;CRY&lt;/em&gt;s, &lt;em&gt;RORα&lt;/em&gt;, and &lt;em&gt;NR1D1&lt;/em&gt; expression were quantified every 6 hours via qPCR. Trophoblast migration/invasion, influenced by BMAL1, were assessed through transwell assay, and related protein expressions (β-catenin, integrin-β1, integrin-ɑ5) were evaluated using western blotting. Statistical significance was determined at p&lt;0.05 using two-way ANOVA and Tukey's post hoc test.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The outcomes from JTK-Cycle analysis, revealed the inherent rhythmic expression pattern of &lt;em&gt;BMAL1&lt;/em&gt; in HVT cells. This natural expression displayed an average period of 23.3 hours and an average amplitude of 429,7 abs. BMAL1 silencing was confirmed at the protein level. Rhythmic expression of other MCGs; &lt;em&gt;CLOCK, PER1/2/3, CRY1/2, RORα&lt;/em&gt;, and &lt;em&gt;NR1D1&lt;/em&gt; genes were altered significantly in &lt;em&gt;BMAL1&lt;/em&gt;-silenced group(p&lt;0.05). Following &lt;em&gt;BMAL1&lt;/em&gt; silencing, a notable reduction in the migration and invasion capabilities of HVT cells was observed(p&lt;0.05). Additionally, &lt;em&gt;BMAL1&lt;/em&gt; depletion correlated with a significant decrease in β-catenin expression from ZT30 onwards, and a decrease in integrin-β1 expression from ZT36 onwards(p&lt;0.05). Conversely, there was a significant increase in integrin-ɑ5 protein expression beginning at ZT24(p&lt;0.05).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;&lt;em&gt;BMAL1&lt;/em&gt; is rhythmically expressed in HVT cells, and its silencing disrupts rhythmic expression of key MCGs and leads to alterations in trophoblast migration and invasion-related pathways. Specifically, &lt;em&gt;BMAL1&lt;","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104557"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECT OF ACETYL L-CARNITINE ADMINISTRATION ON TESTICULAR HISTOPATHOLOGY, APOPTOSIS, AND OXIDATIVE STRESS IN AN EXPERIMENTAL CHRONIC ALCOHOLISM MODEL","authors":"Merve Gorgulu ,&nbsp;Gizem Gamze Tas ,&nbsp;Gamze Zengin ,&nbsp;Hakan Er ,&nbsp;Sevim Ercan Kelek ,&nbsp;Leyla Sati","doi":"10.1016/j.rbmo.2024.104540","DOIUrl":"10.1016/j.rbmo.2024.104540","url":null,"abstract":"<div><h3>Objective</h3><div>Alcohol addiction is recognized as a serious public health issue today. The World Health Organization classifies alcohol as substances that cause both physiological and psychological dependence when consumed over prolonged periods. Studies clearly indicate that chronic alcohol consumption has detrimental effects on the male reproductive system, suggesting that it is a risk factor for male fertility with reproductive and endocrine problems. Acetaldehyde, which is formed by the metabolism of alcohol, causes the production of free radicals when oxidized, which triggers apoptotic processes and oxidative stress. It is known that chronic alcohol use results in oxidative stress. However, various studies have shown that acetyl-L-carnitine (ALCAR) has antioxidant effects on several biological mechanisms under different conditions. Therefore, we aimed to investigate the potential effects of ALCAR administration on sperm motility, testicular histopathology, apoptosis, and oxidative stress in rats chronically exposed to ethanol (EtOH).</div></div><div><h3>Materials and Methods</h3><div>Rats were randomly separated into 4 groups. Over the course of the 4-week period, EtOH (5 g/kg, 25% w/v), ALCAR (50 mg/kg), and EtOH (5 g/kg, 25% w/v) + ALCAR (50 mg/kg) were given to rats by oral gavage daily (n=10/group). However, the control group received only distilled water. Epididymal sperm motility and testicular weight index were measured in line with testicular histopathology and modified Johnsen scoring. TUNEL assay was used for the assessment of the apoptotic index. Total oxidants and antioxidant status were analyzed with ELISA.</div></div><div><h3>Results</h3><div>Sperm motility and testicular modified Johnsen scores were decreased in the EtOH group (p&lt;0.001). However, the EtOH+ALCAR group showed similar levels as to the control group. Even though no significant difference was determined for testicular weight index, immature germ cells spilled into the lumen, vacuolization in the intertubular area, and expansion in the interstitial area were observed in the EtOH group in contrast to normal testicular histology in the EtOH+ALCAR group. Chronic alcohol administration caused a significant increase in the apoptotic index (p&lt;0.05) and oxidative stress (p&lt;0.001). Nevertheless, we detected decreased apoptotic index and oxidative stress levels in the EtOH+ALCAR group compared to the EtOH group.</div></div><div><h3>Conclusion</h3><div>Our results indicate that ALCAR, an antioxidant compound, may have beneficial effects against the damages caused by chronic use of alcohol, such as poor sperm motility, testicular atrophy, and increased oxidative stress. However, the exact efficacy of ALCAR on the treatment for male infertility and metabolic consequences of chronic alcohol consumption needs to be confirmed by further investigations.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104540"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MENOPAUSAL SYMPTOMS AND SYSTEMIC INFLAMMATION: A COMPARATIVE STUDY IN PERIMENOPAUSAL AND POSTMENOPAUSAL WOMEN","authors":"Busra Korpe ,&nbsp;Caner Kose","doi":"10.1016/j.rbmo.2024.104561","DOIUrl":"10.1016/j.rbmo.2024.104561","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate menopausal symptoms and their frequencies while exploring their potential correlation with systemic inflammation in perimenopausal and postmenopausal women.</div></div><div><h3>Materials and Methods</h3><div>The study involved a comparative analysis of menopausal symptoms and systemic inflammation markers between perimenopausal (n=213) and postmenopausal women (n=219). Participants were assessed with the Menopause Rating Scale (MRS) for somatic, urogenital and psychological symptoms and systemic immune-inflammation index (SII) levels. Regression analysis was conducted to identify parameters strongly associated with high somatic and total MRS scores.</div></div><div><h3>Results</h3><div>Total MRS and somatic domain scores were found to be significantly higher in perimenopausal women compared to postmenopausal women. Additionally, perimenopausal women exhibited elevated levels of systemic inflammation, as indicated by higher SII levels. Regression analysis revealed that being perimenopausal, being smoker and having higher SII results were the most significant parameters related to increased somatic scores. In contrast, parity and exercise were found to be associated with lower somatic scores.</div></div><div><h3>Conclusion</h3><div>The findings of this study highlight the heightened somatic symptomatology and systemic inflammation observed in perimenopausal women. Specifically, being in the perimenopausal phase, being a smoker and having elevated systemic inflammation markers were identified as strong factors associated with increased somatic scores. These results underscore the importance of considering systemic inflammation in the management of menopausal symptoms, particularly during the perimenopausal transition.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104561"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IS PROGESTERONE SUPPLEMENTATION REQUIRED FOR LUTEAL PHASE SUPPORT DURING MODIFIED NATURAL CYCLE FROZEN EMBRYO TRANSFER?","authors":"Gönül Özer ,&nbsp;Sevinc Özmen","doi":"10.1016/j.rbmo.2024.104568","DOIUrl":"10.1016/j.rbmo.2024.104568","url":null,"abstract":"<div><h3>Objective</h3><div>Is there a difference in pregnancy outcomes among women undergoing modified natural cycle frozen embryo transfer (mNC-FET) with or without progesterone as luteal phase support (LPS)?</div></div><div><h3>Materials and Methods</h3><div>A retrospective analysis was conducted on 2216 modified natural cycle frozen embryo transfer (mNC-FET) cycles performed at Memorial Sisli Hospital between 2011 and 2023. The study included patients under 38 years of age who underwent top-quality (TQ) or good-quality (GQ) single embryo transfer in mNC-FET cycles. Excluded from the study were medium-quality (MQ) or poor-quality (PQ) embryo transfer cycles, double embryo transfer cycles, and embryo transfer cycles with preimplantation genetic testing for aneuploidy (PGD/A). During the mNC-FET cycles, patients were monitored using transvaginal ultrasonography starting from the second day of the menstrual cycle to determine the day of ovulation. Based on follicle size and hormone levels, ovulation was induced with a single subcutaneous dose of recombinant human chorionic gonadotropin (r-hCG, Ovitrelle 250 µg). The cycles were divided into three separate groups for analysis: Group A (n=493) did not receive any luteal phase support; Group B (n=1327) received 200 mg of vaginal micronized progesterone twice daily; and Group C (n=396) received 200 mg of vaginal micronized progesterone twice daily along with 25 mg of subcutaneous progestin daily. Groups were compared in terms of cycle characteristics and pregnancy outcomes.</div></div><div><h3>Results</h3><div>Demographic and fresh cycle characteristics of the groups were similar. There was no statistically significant difference between the groups regarding pregnancy outcomes. Live birth rates were 58.4% in Group A, 60.8% in Group B and 60.1% in Group C (p=0.650). Clinical pregnancy rates were 65.9%, 69.1% and 68.2%, respectively (p=0.432). Biochemical abortion rates were 4.5%, 6.6% and 5.3%, respectively (p=0.186) and clinical abortion rates were 6.3%, 6.7% and 5.3%, respectively (p=0.828).</div></div><div><h3>Conclusion</h3><div>The study showed that women under 38 years of age undergoing mNC FET had similar pregnancy outcomes with or without the use of progesterone for luteal phase support. Consequently, the administration of progesterone for luteal phase support seems unnecessary. In these cases, hCG given for triggering and the presence of corpus luteum are sufficient for luteal phase support. Randomised controlled trials are needed to support these findings.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104568"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AGE-STRATIFIED ANALYSIS OF ANTI-MüLLERIAN HORMONE (AMH) LEVELS AND THE PREVALENCE OF DIMINISHED OVARIAN RESERVE IN A POPULATION OF 22,920 WOMEN AGED 18-45: A RETROSPECTIVE STUDY","authors":"Kiper Aslan ,&nbsp;Aylin Tunali ,&nbsp;İlayda Tellioğlu ,&nbsp;Isil Kasapoglu ,&nbsp;Gurkan Uncu","doi":"10.1016/j.rbmo.2024.104550","DOIUrl":"10.1016/j.rbmo.2024.104550","url":null,"abstract":"<div><h3>Objective</h3><div>Anti-Müllerian hormone (AMH) is recognized as the most reliable indicator of ovarian reserve and is widely utilized in various clinical settings. AMH concentrations consistently decline as women age. Despite its widespread use, the lack of standardized, age-specific reference ranges for AMH in reproductive-aged women restricts its full clinical utility. This retrospective analysis aims to explore age-stratified AMH levels and establish the prevalence of diminished ovarian reserve (DOR) at different ages.</div></div><div><h3>Methods</h3><div>The study was conducted at a tertiary university hospital. An electronic database was screened for AMH results between the years 2015-2024. All analyzed AMH results were recorded regardless of the purpose of the analysis. Women aged between 18-45 were enrolled, ensuring each patient was only included once to avoid duplications. Correlation analysis was performed to show the relationship between AMH and age. Median AMH values for each age group were calculated, and the prevalence of diminished ovarian reserve according to AMH levels was analyzed. The Poseidon Criteria were used to define diminished ovarian reserve (&lt;1.2 ng/ml).</div></div><div><h3>Results</h3><div>A total of 24,587 AMH results were found in the electronic database for the years 2015-2024. After excluding duplications and results for women older than 45 and younger than 18, 22,920 AMH results were analyzed. Correlation analysis showed a significant relationship between AMH levels and age. As age increased, AMH levels significantly decreased. By age 36, median AMH levels had decreased below 1.2 ng/ml. The prevalence of DOR was 15.9% at age 18, while this ratio was nearly 96% at age 45.</div></div><div><h3>Conclusion</h3><div>This study presented age-stratified AMH levels for a large population of over 20,000 women. Although the study population consisted of patients from a tertiary hospital, the results may be beneficial in establishing age-specific AMH levels in clinical practice.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104550"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VAGINAL MICROBIOME TRANSPLANTATIONS - A POTENTIAL NEW TREATMENT IN REPRODUCTIVE DISEASE","authors":"Henriette Svarre Nielsen","doi":"10.1016/j.rbmo.2024.104508","DOIUrl":"10.1016/j.rbmo.2024.104508","url":null,"abstract":"<div><div>This talk is about the vaginal microbiome and will present studies about vaginal and endometrial dysbiosis and reproductive outcomes. Treatment studies will be presented including the Dyscover studies on vaginal microbiome transplantation.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104508"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF THE RELATIONSHIP BETWEEN OBESITY AND OXIDATIVE STRESS IN PATIENTS WITH ADOLESCENT POLYCYSTIC OVARY SYNDROME","authors":"Cahidenur Selveroğlu ,&nbsp;Banuhan Şahin ,&nbsp;Hanife Kara","doi":"10.1016/j.rbmo.2024.104554","DOIUrl":"10.1016/j.rbmo.2024.104554","url":null,"abstract":"<div><h3>Objective</h3><div>Polycystic ovary syndrome (PCOS) is one of the most common diseases that starts in adolescence and is common in women of reproductive age. PCOS patients have menstrual dysfunction, signs of hyperandrogenism (such as hirsutism, acne), metabolic problems, increased cardiovascular risk, insulin resistance, risk of developing type 2 diabetes mellitus. Oxidative stress; It has high levels in patients with central obesity, insulin resistance and hyperandrogenicity. Human and animal studies on oxidative stress in insulin resistance in Turkey and in the world are insufficient. Based on this information, we aimed to obtain new data on the relationship between oxidative stress parameters, total antioxidant level (TAS), total oxidant level (TOS), and oxidative stress index (OSI) levels, with obesity in adolescence with PCOS.</div></div><div><h3>Materials and Methods</h3><div>Between 01.02.2021 and 01.02.2022, Amasya University Sabuncuoğlu Şerefeddin Education and Research Hospital Preferred to Gynecology and Obstetrics outpatient clinics, four groups were created, 31 normal weight and 32 overweight/obese non-PCOS; 30 normal weight and 32 overweight/obese PCOS. Rotterdam criteria was used for PCOS diagnosis. Research permission and informed consent were obtained from all patients. Blood samples were analyzed for glucose, total testosterone (TT), dehydroepiandrosterone sulfate (DHEA-S), Luteinizing hormone (LH), Follicle stimulant hormone (FSH), estrogen, progesterone, 17OH-progesterone (17OH-P), creatinine and hemogram levels. TAS, TOS and OSI were calculated with TAS and TOS kits and differences between groups were examined.</div></div><div><h3>Result</h3><div>Among the study groups, TT, 17OH-P, DHEA-S, WBC, CRP, ALT values, TAS, TOS and OSI were significantly higher in the obese and PCOS groups. While there is a strong correlation between TOS and OSI; there is a moderate degree between TAS and OSI. As part of the research, especially in the Obese/PCOS group patients, as the WBC value increased compared to other groups, TOS increased; TAS increased as CRP value increased; TOS increased as TT increased; As the ALT value increased, it was found that both TAS and TOS increased. A statistically significant difference in TOS and TAS values was found in the Obese/PCOS groups with Normal Weight/Normal Patient. A statistically significant difference in TAS values was found in the Obese/PCOS binary group comparison with Normal Weight/PCOS.</div></div><div><h3>Conclusion</h3><div>In our study, we observed changes in TOS and OSI levels in overweight/obese and non-overweight/obese adolescences with PCOS and non-PCOS. An inflammatory process in obesity was found to be effective in pathology of adolescence PCOS.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"49 ","pages":"Article 104554"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}