Reproductive biomedicine online最新文献

{"title":"Outside Back Cover - Editorial Board","authors":"","doi":"10.1016/S1472-6483(26)00033-7","DOIUrl":"10.1016/S1472-6483(26)00033-7","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 2","pages":"Article 105492"},"PeriodicalIF":3.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disentangling the current role of LH activity in assisted reproduction: from biology to patient personalization","authors":"José Bellver ,&nbsp;Francisco Fabregues ,&nbsp;Ernesto Bosch ,&nbsp;José Serna ,&nbsp;Juan José Espinós","doi":"10.1016/j.rbmo.2025.105154","DOIUrl":"10.1016/j.rbmo.2025.105154","url":null,"abstract":"<div><div>The importance of determining the precise role of gonadotrophins in assisted reproductive technology (ART) is increasingly recognized, as optimizing ovarian stimulation protocols is crucial for improving outcomes. Recent advances in reproductive biology highlight the multifaceted functions of LH, revealing a complex interplay between its endocrine actions and local ovarian mechanisms. Traditionally, LH was primarily associated with its role in triggering ovulation and supporting the corpus luteum. Accumulating evidence, however, suggests that LH also influences follicular steroidogenesis, oocyte maturation and endometrial receptivity. Despite these insights, the role of LH in ovarian stimulation remains under debate, particularly outside of patients with hypogonadotropic hypogonadism, characterized by hypothalamic amenorrhoea. Notably, recent molecular and clinical evidence supports the benefits of including LH activity in ovarian stimulation protocols for select patient groups. LH activity can be derived from human menopausal gonadotrophin, which contains both FSH and LH activity, with LH primarily driven by HCG; recombinant LH (rLH), or recombinant FSH + LH (rFSH + rLH). This review clarifies the evolving role of LH activity in ART, bridging foundational biological insights with the emerging paradigm of patient-specific treatment strategies to optimize reproductive outcomes.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 2","pages":"Article 105154"},"PeriodicalIF":3.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145477283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can artificial intelligence models provide reliable medical counselling to fertility patients?","authors":"Idan Alcalay ,&nbsp;Ariel Weissman ,&nbsp;Hadas Ganer Herman ,&nbsp;Avi Tsafrir ,&nbsp;Matan Friedman ,&nbsp;Eran Weiner ,&nbsp;Raoul Orvieto ,&nbsp;Nikolaos P Polyzos ,&nbsp;Michael H Dahan ,&nbsp;Alex Polyakov ,&nbsp;Robert Fischer ,&nbsp;Sandro C Esteves ,&nbsp;Baris Ata ,&nbsp;Jason M Franasiak ,&nbsp;Yossi Mizrachi","doi":"10.1016/j.rbmo.2025.105237","DOIUrl":"10.1016/j.rbmo.2025.105237","url":null,"abstract":"<div><h3>Research question</h3><div>Can generative artificial intelligence (AI) models provide reliable counselling to fertility patients regarding real-world clinical questions?</div></div><div><h3>Design</h3><div>In this cross-sectional study, 12 clinical questions were developed to reflect common, real-life dilemmas encountered during fertility workup and treatment. Responses to each question were generated by two experienced fertility specialists, and two AI models – ChatGPT and Gemini. Eight leading internationally recognized fertility experts, blinded to the source of each reply, independently rated all the responses on a scale from 1 (strongly disagree) to 10 (strongly agree). Ratings were compared across all four repliers using non-parametric statistical tests.</div></div><div><h3>Results</h3><div>The replies authored by physicians received significantly higher overall scores than those generated by AI models (<em>P</em> &lt; 0.001). The median scores were highest for Doctor A (9.0), followed by Doctor B (8.0), then ChatGPT (7.0) and finally Gemini, which received the lowest score (4.5). The proportion of high-scoring responses (≥8) was greatest for Doctor A (70.8%), followed by Doctor B (56.3%), then ChatGPT (47.9%) and finally Gemini (35.4%) (<em>P</em> &lt; 0.001).</div></div><div><h3>Conclusions</h3><div>Experienced fertility specialists outperformed generative AI models in providing accurate responses to complex clinical questions. Despite the growing accessibility and sophistication of AI tools, their use for individualized fertility counselling remains limited. Continued refinement and clinical validation of AI tools are essential before they can be considered reliable for patient-specific guidance. At present, AI should be viewed as a complementary resource rather than a substitute for expert clinical judgement.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 2","pages":"Article 105237"},"PeriodicalIF":3.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145878896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trend analysis of the burden of PCOS and related infertility in China and worldwide between 1990 and 2021","authors":"Qianqian Liang","doi":"10.1016/j.rbmo.2025.105239","DOIUrl":"10.1016/j.rbmo.2025.105239","url":null,"abstract":"<div><h3>Research question</h3><div>What is the global and Chinese burden of polycystic ovary syndrome (PCOS) and infertility attributable to PCOS on the basis of age-standardized calculations from 1990 to 2021?</div></div><div><h3>Design</h3><div>Using open data from the Global Burden of Disease (GBD) database from 1990 to 2021, this study analysed the characteristics of PCOS and infertility attributable to PCOS in China and worldwide. Joinpoint was used to calculate the average annual percentage change (AAPC) and the corresponding 95% CI. An autoregressive integrated moving average (ARIMA) model was used to project the burden of disease from 2022 to 2036.</div></div><div><h3>Results</h3><div>From 1990 to 2021, the AAPC of the age-standardized incidence rate (ASIR), age-standardized prevalence rate (ASPR) and age-standardized disability-adjusted life years rate (ASDR) of PCOS in China were 2%, 2% and 2.1%, respectively. Worldwide, the respective figures were 0.8%, 0.8% and 0.8%, respectively. The AAPC for ASPR and age-standardized years lived with disability rate (ASYR) for the global burden of infertility attributable to PCOS were 1% and 0.9%, respectively, compared with 1.9% and 2%, respectively, in China. By 2036, ASIR, ASPR and ASDR of PCOS in China are projected to increase to 71.64/100,000, 1912.27/100,000 and 16.48/100,000, respectively. Globally, these rates are projected to reach 68.64/100,000, 1934.92/100,000 and 16.91/100,000, respectively. ASPR and ASYR for infertility attributable to PCOS are predicted to continue their upward trend.</div></div><div><h3>Conclusion</h3><div>From 1990 to 2021, the burden of PCOS and infertility attributable to PCOS continued to rise in China and globally, making PCOS a major public health problem. Raising public awareness of this common disease in order to reduce complications has become a public health priority that needs attention.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 2","pages":"Article 105239"},"PeriodicalIF":3.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Matter - Continued TOC","authors":"","doi":"10.1016/S1472-6483(26)00022-2","DOIUrl":"10.1016/S1472-6483(26)00022-2","url":null,"abstract":"","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 2","pages":"Article 105481"},"PeriodicalIF":3.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146189085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm insulin-like growth factor 2 protein levels: implications for early embryo development","authors":"Rossella Cannarella ,&nbsp;Oliver J. Rando ,&nbsp;Sandrine Chamayou ,&nbsp;Simona Romano ,&nbsp;Antonino Guglielmino ,&nbsp;Qiangzong Yin ,&nbsp;Francesca Mancuso ,&nbsp;Iva Arato ,&nbsp;Cinzia Lilli ,&nbsp;Stefano Brancorsini ,&nbsp;Sabrina Farinacci ,&nbsp;Giovanni Luca ,&nbsp;Loredana Leggio ,&nbsp;Nunzio Iraci ,&nbsp;Scott D. Lundy ,&nbsp;Sandro La Vignera ,&nbsp;Aldo E. Calogero","doi":"10.1016/j.rbmo.2025.105202","DOIUrl":"10.1016/j.rbmo.2025.105202","url":null,"abstract":"<div><h3>Research question</h3><div>What is the role of sperm-derived insulin-like growth factor 2 (IGF2) protein in early embryo development, and what is its potential influence on outcomes in assisted reproductive technology (ART)?</div></div><div><h3>Design</h3><div>In 27 infertile couples undergoing ART, IGF2 protein levels in spermatozoa were quantified using enzyme-linked immunosorbent assay. Their correlation with embryo morphokinetics was assessed via time-lapse imaging. The analysis was adjusted for female partner’s age, body mass index and serum anti-Müllerian hormone levels. To explore underlying mechanisms, recombinant IGF2 was added to murine parthenote cultures. Transcriptome analysis was conducted at the blastocyst stage.</div></div><div><h3>Results</h3><div>Sperm IGF2 levels were significantly associated with the number of post-gradient sperm recovered (r = 0.65; <em>P</em> &lt; 0.001) and with the timing of pronuclear formation (r = –0.22; <em>P</em> = 0.04), even after adjusting for confounding factors, indicating a role in the earliest stages of embryogenesis. No significant correlation was observed with later stages of embryo development, possibly owing to a developmental transition from paternal to embryonic IGF2 transcript expression. Furthermore, IGF2 levels did not differ significantly among ART outcome groups (biochemical pregnancy, clinical pregnancy and live birth). Transcriptome analysis of IGF2-treated parthenotes revealed enrichment in pathways related to protein binding, which is critical for pronuclear formation.</div></div><div><h3>Conclusion</h3><div>A potential involvement of sperm-delivered IGF2 in early post-fertilization events is suggested, with a limited apparent effect on later embryonic development or ART outcomes. Further investigation into the diagnostic and therapeutic relevance of IGF2 in the context of male-factor infertility is needed.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 2","pages":"Article 105202"},"PeriodicalIF":3.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145978357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of micronutrients on fertility and aneuploidy rates in human conceptions: a systematic review and meta-analysis","authors":"David Schütz ,&nbsp;Kirstine Huong Lettorp ,&nbsp;Isa Amalie Olofsson ,&nbsp;Eva R. Hoffmann","doi":"10.1016/j.rbmo.2025.105261","DOIUrl":"10.1016/j.rbmo.2025.105261","url":null,"abstract":"<div><div>In order to make clinical recommendations based on uncertain evidence, a systematic review and meta-analysis on the influence of micronutrient supplementation on preclinical and clinical reproductive outcomes was undertaken. PubMed and Scopus were searched until 12 November 2024. Parallel-grouped intervention studies with women undergoing fertility treatment with micronutrient supplementation or addition of micronutrient to in-vitro maturation media were included. The primary outcomes were oocyte maturation and chromosome aneuploidy rates, pregnancy rate, miscarriage rate, and live birth rate. Five of 1810 studies were included. In three clinical studies, 326 women underwent fertility treatment with coenzyme Q10 (CoQ10) or control treatment. CoQ10 increased oocyte retrieval and live birth rates for women diagnosed with poor ovarian response (POR; OR = 2.28), and increased the pregnancy rate for women diagnosed with POR or polycystic ovary syndrome (PCOS) (OR = 2.20 and 13.26, respectively). CoQ10 had no effect on the miscarriage rate. In two in-vitro studies, 127 women donated 241 immature oocytes which were matured with CoQ10 or resveratrol. CoQ10 increased the oocyte maturation rate (OR = 2.73), and decreased oocyte and chromosome aneuploidy rates (OR = 0.31 and 0.57, respectively) for women of advanced maternal age (AMA). Resveratrol had no effect. Women of AMA and women diagnosed with POR or PCOS gained greater benefit from CoQ10 supplementation.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 2","pages":"Article 105261"},"PeriodicalIF":3.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145885562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myo-inositol in reproductive management of women with PCOS: holy grail for medical practice or demon for scientific evidence?","authors":"Stefano Palomba ,&nbsp;Giuseppe Seminara ,&nbsp;Antonio Aversa","doi":"10.1016/j.rbmo.2025.105269","DOIUrl":"10.1016/j.rbmo.2025.105269","url":null,"abstract":"<div><div>Myo-inositol has gained widespread use in the management of infertility associated with polycystic ovary syndrome (PCOS), driven by its proposed role in improving insulin sensitivity and ovarian function. Despite its popularity in clinical practice, the evidence supporting myo-inositol’s efficacy in enhancing fertility outcomes remains limited and inconclusive. Current international and national guidelines do not endorse myo-inositol as a first-line treatment for PCOS-related infertility, either as monotherapy or in combination with ovulation induction agents. Even if early studies suggest potential benefits when used alongside clomiphene citrate or gonadotrophins, these findings are often outdated or misaligned with contemporary treatment protocols that favour letrozole and gonadotrophin-releasing hormone antagonist regimens. In the context of IVF, myo-inositol may reduce gonadotrophin requirements and improve some intermediate/secondary outcomes, although data on live births and long-term efficacy are lacking. This commentary critically evaluates the current literature on use of myo-inositol in PCOS fertility care, emphasizing the need for well-designed, adequately powered randomized controlled trials that reflect current clinical standards. Until stronger evidence is available, myo-inositol should not be routinely recommended in fertility protocols for PCOS but reserved for individualized cases within an evidence-based framework.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 2","pages":"Article 105269"},"PeriodicalIF":3.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attributes of a large cohort of human seminal extracellular vesicles with varied sperm features","authors":"Yu-Sheng Cheng ,&nbsp;Hsing-Yi Chen ,&nbsp;Chia-Min Liu ,&nbsp;Hsiu-Chiung Hou ,&nbsp;Yu-Chiao Lin ,&nbsp;Tsung-Yen Lin ,&nbsp;Shih-Chieh Lin","doi":"10.1016/j.rbmo.2025.105233","DOIUrl":"10.1016/j.rbmo.2025.105233","url":null,"abstract":"<div><h3>Research question</h3><div>Do human seminal plasma extracellular vesicles (SPEV) vary with distinct sperm features and possess clinical relevance for male infertility?</div></div><div><h3>Design</h3><div>Semen samples were provided for analysis: normozoospermic (<em>n</em> = 64); oligozoospermic (<em>n</em> = 35); asthenozoospermic (<em>n</em> = 51); teratozoospermic (<em>n</em> = 16); and azoospermic cases (<em>n</em> = 134). Size-exclusion chromatography was used to isolate SPEV, and concentration and size distribution were assessed via nanoparticle tracking analysis. Protein concentrations were measured using BCA Protein Assay Kits. Continuous variables were analysed as mean with a 95% CI.</div></div><div><h3>Results</h3><div>Highest mean SPEV concentration was observed in the normozoospermic group (4.9 × 10¹⁰/ml; 95% CI 3.5 –6.3 × 10¹⁰/ml); lowest mean concentration was found in azoospermic group (0.6 × 10¹⁰/ml; 95% CI 0.5 –0.8 × 10¹⁰/ml). Pearson coefficients of SPEV concentration with sperm count, progressive motility and total motile sperm count were 0.250, 0.344 and 0.433, respectively. Mean SPEV size from infertile men with non-obstructive azoospermia (NOA) is significantly smaller than that from men who have undergone a vasectomy or with obstructive azoospermia (151.3 nm, 95% CI 147.6 to 155.0 nm versus 159.4 nm, 95% CI 154.0 to 164.8 nm, <em>P</em> = 0.049). Mean protein content per SPEV particle is significantly higher in the NOA group than the vasectomy or obstructive azoospermia group (93.41 ag, 95% CI 50.67 to 132.6 ag versus 44.24 ag, 95% CI 24.74 to 63.74 ag, <em>P</em> &lt; 0.001). The AUC curve is 0.702 (<em>P</em> &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>Concentration of SPEV significantly correlates with total motile spermatozoa. Protein content per SPEV particle may be a potential marker to distinguish obstructive azoospermia from NOA.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 2","pages":"Article 105233"},"PeriodicalIF":3.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oocyte-mediated repair of sperm DNA fragmentation: a critical determinant of embryo viability","authors":"Maria Luisa Pardiñas ,&nbsp;Carlos de Celis ,&nbsp;Julia Gil ,&nbsp;David Ortega-Jaen ,&nbsp;Angel Martin ,&nbsp;Amparo Mercader ,&nbsp;Maria Jose de los Santos","doi":"10.1016/j.rbmo.2025.105165","DOIUrl":"10.1016/j.rbmo.2025.105165","url":null,"abstract":"<div><div>DNA repair capacity is a critical biological process for maintaining genomic integrity in cells. However, certain cells, such as spermatozoa, possess limited DNA repair ability and depend on the oocyte for this function during fertilization. Among the various types of DNA damage, single- and double-strand sperm DNA fragmentation has emerged as a key factor influencing embryo development and clinical outcomes. Improperly repaired DNA fragmentation can result in developmental delays, embryo arrest or chromosomal abnormalities. This review explores the capacity of the oocyte to repair sperm DNA damage, and how maternal age affects this critical function. Through a structured literature review, the mechanisms involved in oocyte-mediated DNA repair are described and their clinical implications are evaluated. Findings indicate that oocyte quality, which diminishes with age, significantly impacts the efficiency of DNA repair processes, thereby influencing embryo viability and pregnancy success. While younger oocytes can partially compensate for sperm DNA fragmentation, aging oocytes show reduced repair capacity, contributing to poor reproductive outcomes. These insights emphasize the need for novel reproductive strategies aimed at enhancing oocyte quality and repair efficiency.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"52 2","pages":"Article 105165"},"PeriodicalIF":3.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145459347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}