Reproductive biomedicine online最新文献_第6页

Outside Back Cover - Editorial Board 封底外-编辑委员会

IF 3.7 2区医学

Reproductive biomedicine online Pub Date : 2025-03-01 DOI: 10.1016/S1472-6483(25)00100-2

引用次数: 0

Characterization of tubulin post-translational modifications and their enzymes during human oocyte meiosis 人类卵母细胞减数分裂过程中微管蛋白翻译后修饰及其酶的特征

IF 3.7 2区医学

Reproductive biomedicine online Pub Date : 2025-03-01 DOI: 10.1016/j.rbmo.2024.103885

Paraskevi Karamtzioti , Anna Ferrer-Vaquer , Montserrat Barragan , Isabelle Vernos , Rita Vassena , Gustavo Tiscornia

{"title":"Characterization of tubulin post-translational modifications and their enzymes during human oocyte meiosis","authors":"Paraskevi Karamtzioti , Anna Ferrer-Vaquer , Montserrat Barragan , Isabelle Vernos , Rita Vassena , Gustavo Tiscornia","doi":"10.1016/j.rbmo.2024.103885","DOIUrl":"10.1016/j.rbmo.2024.103885","url":null,"abstract":"<div><h3>Research question</h3><div>What are the profiles of tubulin post-translational modifications (PTM) in the meiotic spindles of oocytes with different developmental competence: in-vivo matured MII oocytes (IVO) versus cumulus-free in-vitro matured germinal vesicles (IVM)?</div></div><div><h3>Design</h3><div>Cumulus-free germinal vesicles were matured <em>in vitro</em> and compared with IVO in terms of their tubulin PTM, characterizing tubulin PTM patterns (acetylation, tyrosination, detyrosination, D2-tubulin formation and polyglutamylation) of their meiotic spindles by immunofluorescence and by evaluation of the expression levels of the enzymes involved by quantitative reverse transcription polymerase chain reaction.</div></div><div><h3>Results</h3><div>Levels of D2-tubulin, tyrosination and polyglutamylation were similar in IVO and IVM oocytes; acetylation displayed different patterns in both groups, whereas detyrosination was detected only in MII oocytes matured <em>in vivo</em>. The PTM enzyme RNA expression levels in germinal vesicles, IVO and IVM were analysed, as well as in oocytes that failed to mature. Of the 24 PTM enzyme transcripts tested, 17 were present in at least one of the groups under study (<em>aΤΑΤ1, NAA50, HDAC6, SIRT2, TTL, TTLL1, TTLL2, TTLL4, TTLL5, TTLL6, TTLL9, TTLL11, TTLL12, CCP1, CCP5, CCP6, VASH1</em>), of which three were differentially expressed (<em>NAA50, TTLL12, CCP1</em>).</div></div><div><h3>Conclusion</h3><div>All modifications studied were present in human oocytes regardless of whether their meiotic maturation occurred <em>in vivo</em> or <em>in vitro</em>, except for detyrosination, which was found at low levels in the IVO group, but not in the IVM group. This suggests that the presence of tubulin PTMs may play a role in human oocyte maturation, completion of meiosis and developmental competence.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 103885"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139821182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The investigation and management of recurrent early pregnancy loss: a Canadian Fertility and Andrology Society clinical practice guideline 复发性早期妊娠丢失的调查和处理：加拿大生育和男科学会临床实践指南

IF 3.7 2区医学

Reproductive biomedicine online Pub Date : 2025-03-01 DOI: 10.1016/j.rbmo.2024.104456

Sony Sierra , Jason Min , Julio Saumet , Heather Shapiro , Camille Sylvestre , Jeff Roberts , Kimberly Liu , William Buckett , Maria P. Velez , Neal Mahutte

引用次数: 0

ICSI for non-male infertility: from ineffectiveness to gender bias? 非男性不育的ICSI：从无效到性别偏见？

IF 3.7 2区医学

Reproductive biomedicine online Pub Date : 2025-03-01 DOI: 10.1016/j.rbmo.2024.104706

Edgardo Somigliana , Giussy Barbara , Laila G. Micci , Giorgia Di Stefano , Alessio Paffoni , Paola Vigano

{"title":"ICSI for non-male infertility: from ineffectiveness to gender bias?","authors":"Edgardo Somigliana , Giussy Barbara , Laila G. Micci , Giorgia Di Stefano , Alessio Paffoni , Paola Vigano","doi":"10.1016/j.rbmo.2024.104706","DOIUrl":"10.1016/j.rbmo.2024.104706","url":null,"abstract":"<div><div>Intracytoplasmic sperm injection (ICSI) is currently being abused as it is also frequently employed in the absence of a frank male cause of infertility. There is growing and robust evidence showing that the use of ICSI should be limited to couples with male infertility. For all other causes of infertility, the procedure does not increase the chance of live birth, may increase malformations in newborns and requires more resources than conventional IVF. In addition, the use of ICSI may impact the sex ratio at birth, favouring female infants. Even if this effect may not be targeted as a gender biased procedure, it should be seen as a wake-up and warning call. It reminds us that ICSI is a ‘non-natural’ invasive technique that circumvents natural sperm selection mechanisms. Overall, a policy of indiscriminate use of ICSI is not justified and a plea is made for a return to a higher use of conventional IVF. Fear about failed fertilization or the use of preimplantation genetic testing for aneuploidies may represent barriers to the need to lower ICSI use and should be overcome.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104706"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to ‘Biological characteristics related to treatment effects of the levonorgestrel-releasing intrauterine system on adenomyosis-associated dysmenorrhoea’ Reproductive BioMedicine Online, 2024, 49;6:104393 《左炔诺孕酮宫内释放系统治疗腺肌病相关性痛经的生物学特性》《生殖生物医学在线》，2024,49;6:104393。

IF 3.7 2区医学

Reproductive biomedicine online Pub Date : 2025-03-01 DOI: 10.1016/j.rbmo.2025.104829

Qiutong Li , Dai Yi , Xiaoyan Li , Yushi Wu , Zhiyue Gu , Chenyu Zhang , Hailan Yan , Shiqing Lyu , Biyun Zhang , Jinghua Shi , Jinhua Leng

引用次数: 0

Inside Front Cover - Affiliations and First page of TOC 内部封面-附属机构和第一页的TOC

IF 3.7 2区医学

Reproductive biomedicine online Pub Date : 2025-03-01 DOI: 10.1016/S1472-6483(25)00090-2

引用次数: 0

Front Matter - Continued TOC 正面物质-继续TOC

IF 3.7 2区医学

Reproductive biomedicine online Pub Date : 2025-03-01 DOI: 10.1016/S1472-6483(25)00091-4

引用次数: 0

Corrigendum to ‘Successful live birth in women with partial 17α-hydroxylase deficiency: report of two cases’ [Reproductive BioMedicine Online (2024) Volume 49, Issue 2, 103855] 对“部分17α-羟化酶缺乏症妇女成功活产：两例报告”的更正[生殖生物医学在线（2024）卷49，第2期，103855]。

IF 3.7 2区医学

Reproductive biomedicine online Pub Date : 2025-03-01 DOI: 10.1016/j.rbmo.2024.104780

Xiaofang Du, Qi Jia, Sheling Wu, Bijun Wang, Yichun Guan

引用次数: 0

Derivative and non-derivative aneuploidy rates in PGT tested blastocysts from carriers of structural rearrangements 结构重排携带者的PGT囊胚的衍生性和非衍生性非整倍体率。

IF 3.7 2区医学

Reproductive biomedicine online Pub Date : 2025-03-01 DOI: 10.1016/j.rbmo.2024.104407

Lauren Walters-Sen, Dana Neitzel, Rachel E. Ellsworth, Sarah Poll, Nicole Faulkner, Swaroop Aradhya

{"title":"Derivative and non-derivative aneuploidy rates in PGT tested blastocysts from carriers of structural rearrangements","authors":"Lauren Walters-Sen, Dana Neitzel, Rachel E. Ellsworth, Sarah Poll, Nicole Faulkner, Swaroop Aradhya","doi":"10.1016/j.rbmo.2024.104407","DOIUrl":"10.1016/j.rbmo.2024.104407","url":null,"abstract":"<div><h3>Research question</h3><div>What is the likelihood of having an euploid embryo when undergoing preimplantation genetic testing for structural rearrangements (PGT-SR)?</div></div><div><h3>Design</h3><div>PGT-SR data from 364 couples (2822 trophectoderm biopsies) with a reciprocal translocation (RecT, <em>n</em> = 263), Robertsonian translocation (RobT, <em>n</em> = 79) or inversion (Inv, <em>n</em> = 22) were analysed retrospectively. Rates of euploid, derivative aneuploid or non-derivative aneuploid were evaluated for each cycle, stratified by the type of rearrangement and parent of origin.</div></div><div><h3>Results</h3><div>Inv had the highest rate of euploid embryos (47.0–52.5%), followed by RobT (34.1–45.2%) and RecT (24.0–28.2%). The rates of euploid embryos were significantly lower for carriers of RobT and RecT compared with age-matched controls (57.6–59.0%). Maternal versus paternal rearrangements had significantly higher rates of derivative-abnormal findings for RobT (41.6% versus 20.2%) and RecT (60.2% versus 52.7%). Aneuploidies involving other chromosomes did not differ significantly in frequency between rearrangement carriers (38.1–41.9%) and age-matched controls (40.6–42.4%).</div></div><div><h3>Conclusions</h3><div>Data from this study demonstrated that Inv carriers have the highest rates of euploid embryos among all carriers of chromosomal rearrangements, that maternal rearrangements confer a higher risk of abnormal embryos, and that evidence for an interchromosomal effect on aneuploidy rates was not present in this cohort. This analysis of over 2700 PGT-SR biopsies enabled generation of likelihood-of-transfer tables stratified by type of translocation, parent of origin, and number of biopsies, which can be used to help counsel patients pursuing PGT-SR.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 3","pages":"Article 104407"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143410390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced endometrial glycolysis concomitant with increased lesional fibrosis in patients with adenomyosis who complained of heavy menstrual bleeding 主诉月经出血量大的子宫腺肌症患者的子宫内膜糖酵解减少，同时病变纤维化增加。

IF 3.7 2区医学

Reproductive biomedicine online Pub Date : 2025-02-01 DOI: 10.1016/j.rbmo.2024.104406

Chenyu Mao , Xishi Liu , Sun-Wei Guo

{"title":"Reduced endometrial glycolysis concomitant with increased lesional fibrosis in patients with adenomyosis who complained of heavy menstrual bleeding","authors":"Chenyu Mao , Xishi Liu , Sun-Wei Guo","doi":"10.1016/j.rbmo.2024.104406","DOIUrl":"10.1016/j.rbmo.2024.104406","url":null,"abstract":"<div><h3>Research question</h3><div>What role, if any, does the extent of lesional fibrosis play in impaired glycolysis leading to adenomyosis-associated heavy menstrual bleeding (ADM-HMB)?</div></div><div><h3>Design</h3><div>Forty-eight patients with ADM-HMB were recruited, among them 25 reported moderate to heavy bleeding (MHB), and the remaining 23, excessive bleeding (EXB). The full-thickness uterine tissue columns were processed for Masson trichrome staining and immunohistochemistry analyses. The expression levels of HIF-1α, GLUT1, HK2, PFKFB3 and PKM2 proteins that are critically involved in glycolysis in endometrial epithelial cells cultured on substrates of different stiffness, and the levels of glycolysis were quantitated. A mouse experiment with induced adenomyosis and simulated menstrual bleeding was conducted to assess the effect of adenomyosis on immunoexpression of proteins involved in glycolysis and inflammation as well as on endometrial repair and bleeding<em>.</em></div></div><div><h3>Results</h3><div>The endometrial staining of HIF-1α, GLUT1, HK2, PFKFB3 and PKM2 was significantly lower in the EXB group as compared with MHB patients, concomitant with higher extent of fibrosis. The expression of HIF-1α, GLUT1, HK2, PFKFB3 and PKM2 was significantly reduced when endometrial epithelial cells were cultured in stiff substrate, concomitant with reduced glycolysis. Mice with induced adenomyosis had reduced immunoexpression of Hif-1α, as well as those proteins each of which plays a vital, rate-limiting role in different steps of the glycolysis pathway, such as Glut1, Hk2, Pfkfb3 and Pkm2, and elevated fibrosis in endometrium, concomitant with disrupted endometrial repair and more bleeding.</div></div><div><h3>Conclusions</h3><div>Lesional fibrosis results in reduced endometrial glycolysis in eutopic endometrium and subsequent imbalance in pro-inflammatory and anti-inflammatory response, leading to ADM-HMB.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":"50 2","pages":"Article 104406"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0