Reproductive Medicine and Biology最新文献_第3页

Utilization of Fertility Treatment in Japan During the First Year of Insurance Coverage: Analysis of Real-World Health Claims Data. 日本在保险覆盖的第一年生育治疗的利用：现实世界健康索赔数据的分析。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-03-06 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70033

Makoto Okawara, Tomoichiro Kuwazuru, Moe Masunaga, Kenji Fujimoto, Masako Nagata, Takeshi Iwasa, Yoshihisa Fujino

{"title":"Utilization of Fertility Treatment in Japan During the First Year of Insurance Coverage: Analysis of Real-World Health Claims Data.","authors":"Makoto Okawara, Tomoichiro Kuwazuru, Moe Masunaga, Kenji Fujimoto, Masako Nagata, Takeshi Iwasa, Yoshihisa Fujino","doi":"10.1002/rmb2.70033","DOIUrl":"https://doi.org/10.1002/rmb2.70033","url":null,"abstract":"Purpose: In April 2022, fertility treatments, including assisted reproductive technology (ART), became eligible for public health insurance coverage in Japan. This study describes the prevalence and characteristics of infertility diagnoses and fertility treatments during the first year of coverage.Methods: This study analyzed claims data from 14 health insurance associations from April 2022 to March 2023. Infertility diagnoses and fertility treatments, and related comorbidities were identified via diagnosis, procedure, and drug codes.Results: Among 590,006 women and 619,551 men, the highest prevalence of infertility was seen in women (3.9%) and men (0.5%) aged 30-34 years. General fertility treatment was more common among younger women but decreased with age, while ART became more prevalent after age 35. The major prescriptions were hormone preparations for ART and luteal insufficiency among women. The diagnosis ratio of OHSS was higher than previously reported, with 8.0% to 9.6% of women undergoing fertility treatment, particularly receiving ART and younger. Male prevalence of diagnosis and treatment remained low, with multiple factors underlying access to treatment.Conclusions: Public insurance coverage improved access to fertility treatment and enhanced data standardization in Japan. This study indicates the need for targeted strategies to broaden equity in reproductive health services.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70033"},"PeriodicalIF":3.3,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12965183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Successful Live Birth After Double Fertility Preservation With Embryo Cryopreservation and MPA Therapy Combined With Hysteroscopic Resection for Metachronous Breast and Endometrial Cancer in Women With Cowden Syndrome: A Case Report. 双生育保存结合胚胎低温保存和MPA治疗联合宫腔镜切除异时性乳腺癌和子宫内膜癌后成功活产一例。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-03-05 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70035

Itsuki Kajimura, Michio Kitajima, Shoko Miura, Chiaki Eishi, Riko Matsuda, Kanako Matsumoto, Ayumi Harada, Yuriko Kitajima, Yuri Hasegawa, Megumi Matsumoto, Keitaro Matsumoto, Kiyonori Miura

{"title":"A Successful Live Birth After Double Fertility Preservation With Embryo Cryopreservation and MPA Therapy Combined With Hysteroscopic Resection for Metachronous Breast and Endometrial Cancer in Women With Cowden Syndrome: A Case Report.","authors":"Itsuki Kajimura, Michio Kitajima, Shoko Miura, Chiaki Eishi, Riko Matsuda, Kanako Matsumoto, Ayumi Harada, Yuriko Kitajima, Yuri Hasegawa, Megumi Matsumoto, Keitaro Matsumoto, Kiyonori Miura","doi":"10.1002/rmb2.70035","DOIUrl":"10.1002/rmb2.70035","url":null,"abstract":"Case: Cowden syndrome is an autosomal-dominantly inherited rare condition caused by germline pathogenic variants of the PTEN gene. Multiple tumor development at a younger age in this syndrome may warrant different modalities of fertility preservation. Here we report that women with this syndrome gave successful live birth with frozen-thaw embryo transfer that was cryopreserved before the start of chemotherapy for breast cancer. This woman also suffered from endometrial cancer that developed during hormonal treatment with GnRH agonist and tamoxifen for breast cancer. The lesion was well controlled with oral MPA therapy and hysteroscopic resection.Outcome: She could receive fertility preservation for breast cancer and endometrial cancer before fertility treatment and pregnancy.Conclusion: This is the first report of live births after multiple fertility preservation therapy for metachronous breast and uterine cancer in Cowden syndrome. Appropriate genetic counseling may help the patient's decision making. The treatment plan should be discussed with diverse professionals such as oncologists, reproductive physicians, perinatologists, geneticists, and genetic counselors.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70035"},"PeriodicalIF":3.3,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12963780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilizing Artificial Intelligence in Cine Magnetic Resonance Imaging Analysis: A Promising Approach for Assessment of Uterine Factors and Prediction of Pregnancy Outcomes in Patients With Recurrent Implantation Failure. 人工智能在磁共振成像分析中的应用：一种有前途的方法来评估子宫因素和预测反复植入失败患者的妊娠结局。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-03-02 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70028

Daiki Hiratsuka, Katsuhiko Noda, Kaname Yoshida, Mayu Kinoshita, Yumiko Doi, Okikaze Kato, Kotaro Oshima, Shizu Aikawa, Chihiro Ishizawa, Yamato Fukui, Takehiro Hiraoka, Mitsunori Matsuo, Tomoko Makabe, Gentaro Izumi, Kenbun Sone, Miyuki Harada, Yasushi Hirota

{"title":"Utilizing Artificial Intelligence in Cine Magnetic Resonance Imaging Analysis: A Promising Approach for Assessment of Uterine Factors and Prediction of Pregnancy Outcomes in Patients With Recurrent Implantation Failure.","authors":"Daiki Hiratsuka, Katsuhiko Noda, Kaname Yoshida, Mayu Kinoshita, Yumiko Doi, Okikaze Kato, Kotaro Oshima, Shizu Aikawa, Chihiro Ishizawa, Yamato Fukui, Takehiro Hiraoka, Mitsunori Matsuo, Tomoko Makabe, Gentaro Izumi, Kenbun Sone, Miyuki Harada, Yasushi Hirota","doi":"10.1002/rmb2.70028","DOIUrl":"https://doi.org/10.1002/rmb2.70028","url":null,"abstract":"Purpose: Recurrent implantation failure (RIF) is a form of refractory infertility that persists despite assisted reproductive technology. Cine magnetic resonance imaging (cine MRI) enables the visualization of uterine peristalsis; however, its use in RIF assessment is limited due to the lack of a standardized application method. This study aimed to develop pregnancy prediction models for patients with RIF and to evaluate the utility of cine MRI image analysis using artificial intelligence (AI).Methods: We retrospectively analyzed the anonymized clinical data and cine MRI images of 188 patients with RIF and known pregnancy outcomes. Two types of models, based on clinical data only or both clinical data and cine MRI images, were built using the Random Forest model. The best model was identified using the area under the receiver operating characteristic curve (AUC), accuracy, sensitivity, and specificity.Results: Higher performance was achieved using the Random Forest model integrating clinical data and cine MRI images (AUC/accuracy/sensitivity/specificity: 0.835, 0.754, 0.879, and 0.583, respectively), outperforming models using clinical data only (0.617, 0.596, 0.697, and 0.458, respectively).Conclusions: AI analysis of clinical data combined with cine MRI data improved pregnancy prediction, suggesting that cine MRI can be used to evaluate uterine factor-related RIF.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70028"},"PeriodicalIF":3.3,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12951816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147348976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TFAM-Mediated mtDNA Replication is Essential for Developmental Competence of In Vitro Grown Oocytes. tfam介导的mtDNA复制对体外培养卵母细胞的发育能力至关重要。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-02-23 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70031

Son Quang Do, Hidetaka Tasaki, Hiroaki Funahashi, Takuya Wakai

{"title":"TFAM-Mediated mtDNA Replication is Essential for Developmental Competence of In Vitro Grown Oocytes.","authors":"Son Quang Do, Hidetaka Tasaki, Hiroaki Funahashi, Takuya Wakai","doi":"10.1002/rmb2.70031","DOIUrl":"https://doi.org/10.1002/rmb2.70031","url":null,"abstract":"Purpose: Mitochondria are essential for oocyte maturation and early embryonic development, supplying ATP and maintaining mitochondrial DNA (mtDNA) integrity. During oogenesis, mtDNA undergoes dramatic amplification, but the mechanisms and functional significance of this process remain unclear. The purpose of this study was to elucidate the role of mitochondrial transcription factor A (TFAM) in mouse oocytes using an in vitro growth (IVG) system.Methods: Oocytes at different growth stages were analyzed for mtDNA copy number and expression of mitochondrial biogenesis genes. To assess TFAM function, siRNA targeting Tfam was microinjected into secondary follicles, which were then cultured for 12 days under IVG conditions. Following culture, oocyte growth, mtDNA content, mitochondrial membrane potential, and developmental competence after in vitro fertilization (IVF) were evaluated.Results: mtDNA copy number increased nonlinearly during oocyte growth, with a pronounced rise at the secondary follicle stage accompanied by TFAM upregulation. TFAM knockdown reduced mtDNA copy number and mitochondrial function without affecting oocyte size or meiotic maturation, but significantly decreased blastocyst formation and total cell numbers per blastocyst.Conclusions: TFAM-mediated mtDNA replication is crucial for mitochondrial function and developmental competence of IVG-derived oocytes, underscoring its importance in early embryonic development.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70031"},"PeriodicalIF":3.3,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12928050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147285065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating Unilateral Ovarian Endometrioma's Effect on Ova and Embryo Quality in 409 Infertile Endometriotic Patients. 单侧卵巢子宫内膜瘤对409例不孕子宫内膜异位症患者卵子和胚胎质量影响的研究。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-02-22 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70034

Saeed Alborzi, Ziba Zahiri Sorouri, Elham Askary, Zahra Sonboleh, Setareh Alavi, Tahereh Poordast, Alimohammad Keshtvarz Hesam Abadi

{"title":"Investigating Unilateral Ovarian Endometrioma's Effect on Ova and Embryo Quality in 409 Infertile Endometriotic Patients.","authors":"Saeed Alborzi, Ziba Zahiri Sorouri, Elham Askary, Zahra Sonboleh, Setareh Alavi, Tahereh Poordast, Alimohammad Keshtvarz Hesam Abadi","doi":"10.1002/rmb2.70034","DOIUrl":"https://doi.org/10.1002/rmb2.70034","url":null,"abstract":"Purpose: In women with unilateral endometriosis, how do ovarian endometriomas and their inflammatory content affect oocyte and embryo quality in affected versus healthy ovaries, and what is the impact on ART outcomes?Methods: This study, conducted from March 2022 to March 2024 at Shiraz University of Medical Sciences, assessed infertility outcomes in patients with unilateral endometriomas. It analyzed reproductive parameters (total follicles, oocytes, and embryos quantity and quality) from affected and healthy ovaries, while considering endometrioma size and AMH levels.Results: This study analyzed ovum pickups and ART outcomes in 409 women with unilateral ovarian endometriosis, revealing critical insights into reproductive performance. Participants had an average age of 33.55 years and a basal AMH level of 2.26 ng/dL, categorized into poor (25.2%), normal (57.2%), and high (17.6%) responders based on their AMH levels. Comparison of outcomes showed that healthy ovaries yielded significantly more MII oocytes, total embryos, and Grade C embryos than affected ovaries, although no significant differences were found across various endometrioma sizes regarding follicular development and egg/embryo quality (p > 0.05). Notably, the good responder group outperformed others in terms of GV oocytes, MII oocytes, and total embryos retrieved. Ultimately, among 40 embryo transfers conducted, 21 resulted in successful pregnancies (52.5%).Conclusion: Endometriomas lead to fewer M2, total fertilization rate. However, embryo quality remains unchanged. Future studies should compare ART outcomes in operated and non-operated ovaries and assess deep infiltrating endometriosis's impact on egg quality and quantity.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70034"},"PeriodicalIF":3.3,"publicationDate":"2026-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12927934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147285038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and Determinants of Low Serum Vitamin D Among Women Attending Infertility Clinics in Japan: A Real-World Multicenter Cross-Sectional Study. 在日本不孕不育诊所就诊的妇女中，低血清维生素D的患病率和决定因素：一项真实世界的多中心横断面研究。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-02-22 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70032

Kuniaki Ota, Toshihiro Habara, Nobuo Koyama, Yoshiharu Nakaoka, Takeshi Kuramoto, Mamoru Seki, Hiroaki Yoshida, Takashi Kuno, Motoo Nabeta, Takahito Miyake, Masayuki Kinutani

{"title":"Prevalence and Determinants of Low Serum Vitamin D Among Women Attending Infertility Clinics in Japan: A Real-World Multicenter Cross-Sectional Study.","authors":"Kuniaki Ota, Toshihiro Habara, Nobuo Koyama, Yoshiharu Nakaoka, Takeshi Kuramoto, Mamoru Seki, Hiroaki Yoshida, Takashi Kuno, Motoo Nabeta, Takahito Miyake, Masayuki Kinutani","doi":"10.1002/rmb2.70032","DOIUrl":"https://doi.org/10.1002/rmb2.70032","url":null,"abstract":"Purpose: To estimate the prevalence and correlates of low serum 25-hydroxyvitamin D [25(OH)D] among women attending infertility clinics in Japan.Methods: We conducted a multicenter cross-sectional study of 17 261 women with measured 25(OH)D. Deficiency and insufficiency were defined as < 20 and < 30 ng/mL. Monthly/seasonal variation was assessed, and multivariable logistic regression evaluated correlates of deficiency. In a complete-case subset with vitamin D supplement and multivitamin information, 25(OH)D levels and 3-category status (deficient/insufficient/sufficient) were compared between users and non-users. The association between diminished ovarian reserve (DOR) and vitamin D status categories was assessed using the chi-square test.Results: Median 25(OH)D was 16.4 ng/mL; 66.8% were deficient and 89.0% insufficient. Levels varied by month and season (p < 0.001). Higher BMI was associated with higher odds of deficiency, whereas older age was associated with lower odds. Supplement users had higher 25(OH)D and a more favorable 3-category distribution (p < 0.001); notably, 94.0% of supplement-free women were insufficient (< 30 ng/mL). Vitamin D status categories were not significantly associated with DOR (p = 0.238).Conclusions: Low 25(OH)D is highly prevalent in infertility care in Japan with marked seasonal variation and adiposity-related vulnerability. Supplement use was associated with higher 25(OH)D but did not explain the overall high burden.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70032"},"PeriodicalIF":3.3,"publicationDate":"2026-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12928063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147285030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Risk Factors Affecting Cumulative Live Birth in Couples With Unexplained Infertility Undergoing IVF/ICSI. 不明原因不孕夫妇行体外受精/ICSI累加活产的危险因素分析

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-02-16 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70025

Ran Liu, Tong Wu, Guoyu Ding, Yang Song, Zengxiang Ma, Yujie Dang, Jinlong Ma

{"title":"Analysis of Risk Factors Affecting Cumulative Live Birth in Couples With Unexplained Infertility Undergoing IVF/ICSI.","authors":"Ran Liu, Tong Wu, Guoyu Ding, Yang Song, Zengxiang Ma, Yujie Dang, Jinlong Ma","doi":"10.1002/rmb2.70025","DOIUrl":"https://doi.org/10.1002/rmb2.70025","url":null,"abstract":"Purpose: To identify the specific risk factors associated with unexplained infertility (UI) among infertile couples.Methods: We conducted a retrospective cohort study analyzing UI risk factors, focusing on 5465 infertile couples who completed their first full in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycle out of a total cohort of 62201 infertile couples.Results: Among 5465 UI couples, 3400 achieved a live birth (cumulative live birth rate = 62.2%). Multivariate logistic regression indicated the key negative predictors included: female age ≥ 35 years (odds ratio [OR]: 0.63; 95% confidence interval [CI]: 0.51-0.78; p < 0.001), male age (OR: 0.98; 95% CI: 0.96-0.99; p = 0.015), Female body mass index ≥ 27.5 kg/m2 (OR: 0.78; 95% CI: 0.62-0.97; p = 0.028), low anti-Müllerian hormone (AMH) (AMH < 1.2 ng/mL) (OR: 0.64; 95% CI: 0.52-0.77; p < 0.001), and a mid-range endometrial thickness (EMT) (EMT 0.7-1.0 cm) (OR: 0.77; 95% CI: 0.66-0.89; p < 0.001). A higher number of retrieved oocytes positively correlated with cumulative live birth (CLB) (OR: 1.02; 95% CI: 1.01-1.04; p = 0.039).Conclusion: The prevalence of UI was 8.8% in our population, with 62.2% achieving CLB after one complete IVF/ICSI cycle. Female age and ovarian reserve indicators emerged as the main factors influencing oocyte yield, embryo acquisition, and CLB outcomes in UI patients.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70025"},"PeriodicalIF":3.3,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12907981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146214064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a Novel Human Endometrial Organoid. 一种新型人子宫内膜类器官的建立。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-02-16 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70030

Toshihide Yoneda, Isao Tamura, Azumi Yoshimura, Marina Ito, Amon Shiroshita, Taishi Fujimura, Yuichiro Shirafuta, Shun Sato, Norihiro Sugino

{"title":"Establishment of a Novel Human Endometrial Organoid.","authors":"Toshihide Yoneda, Isao Tamura, Azumi Yoshimura, Marina Ito, Amon Shiroshita, Taishi Fujimura, Yuichiro Shirafuta, Shun Sato, Norihiro Sugino","doi":"10.1002/rmb2.70030","DOIUrl":"https://doi.org/10.1002/rmb2.70030","url":null,"abstract":"Purpose: Human implantation is difficult to study in vivo because of ethical constraints, making the development of in vitro models essential for mechanistic investigations. Here, we applied the technique previously established in mice to develop a novel human endometrial organoid.Methods: Human endometrial tissues were digested and cultured as adherent monolayers without extracellular matrix (ECM). After 10 days, self-organized aggregates appeared and were transferred to low-attachment plates for three-dimensional culture. To assess hormonal responsiveness, organoids were treated with medroxyprogesterone acetate and cyclic adenosine monophosphate for 4 days.Results: The resulting organoids consisted of EpCAM-positive epithelial cells forming an outer layer and PDGFRα-positive stromal cells occupying the inner region. MUC1 and acetylated α-tubulin, proteins localized on the apical surface of endometrial epithelial cells in vivo, were expressed on the outer surface of the epithelial layer in our organoids. Hormonal stimulation altered the expression of receptivity markers, including increased PAEP and decreased PGR in epithelial cells and increased FOXO1 in both epithelial and stromal cells, reflecting in vivo implantation-phase responses.Conclusion: We established a novel ECM-free human endometrial organoid that recapitulates key structural and hormonal characteristics of the human endometrium, providing a promising platform for studying human implantation mechanisms.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70030"},"PeriodicalIF":3.3,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caffeine Supplementation Enhances Aged Human Oocyte Quality and Embryo Development in In Vitro Fertilization: A Retrospective Paired Study. 在体外受精中补充咖啡因可提高老年人类卵母细胞质量和胚胎发育：一项回顾性配对研究。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-02-13 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70029

Jinkyeong Ha, Soyoung Kim, Hyunmee Jang, Yoo Ra Ko, Seyul Han, Woo Sik Lee, Jin Hee Eum

{"title":"Caffeine Supplementation Enhances Aged Human Oocyte Quality and Embryo Development in In Vitro Fertilization: A Retrospective Paired Study.","authors":"Jinkyeong Ha, Soyoung Kim, Hyunmee Jang, Yoo Ra Ko, Seyul Han, Woo Sik Lee, Jin Hee Eum","doi":"10.1002/rmb2.70029","DOIUrl":"10.1002/rmb2.70029","url":null,"abstract":"Purpose: To evaluate whether in vitro caffeine supplementation can improve spindle integrity, embryo development, and clinical outcomes in IVF.Methods: This retrospective paired-cohort study included 704 women aged ≥ 35 years who underwent two ICSI cycles (one caffeine-supplemented and one non-caffeine) within 12 months. Oocytes in caffeine cycles were exposed to 1.25 mM caffeine for 4 h before fertilization. Outcomes included spindle positioning, embryo quality, blastocyst development and euploidy rates, and neonatal outcomes from 20 women (24 neonates). Paired comparisons were performed using the Wilcoxon signed-rank and McNemar's tests.Results: Caffeine cycles showed higher rates of normal spindle positioning (80.2% vs. 61.2%, p < 0.001), high-quality cleavage embryos (63.2% vs. 53.6%, p < 0.001), high-quality blastocysts (39.2% vs. 26.1%, p < 0.001), and euploid embryos (22.9% vs. 10.9%, p = 0.002) than non-caffeine cycles. The fertilization rates were similar between the groups (81.4% vs. 80.8%, p = 0.628). Clinical pregnancy rate was 39.1%, and neonatal outcomes were reassuring.Conclusions: Short-term in vitro caffeine exposure may enhance oocyte and embryo competence in older women undergoing IVF, especially those with prior IVF failures. These findings support caffeine as a simple, practical laboratory intervention. Prospective multicenter studies are warranted to confirm efficacy and safety.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70029"},"PeriodicalIF":3.3,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12903026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146202497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From the Understanding of Maternal Molecules and Mechanisms to Predicting Embryonic Development. 从了解母体分子及其机制到预测胚胎发育。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-02-10 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70026

Yubao Wei, Akihiro Inoue, Kei Miyamoto

{"title":"From the Understanding of Maternal Molecules and Mechanisms to Predicting Embryonic Development.","authors":"Yubao Wei, Akihiro Inoue, Kei Miyamoto","doi":"10.1002/rmb2.70026","DOIUrl":"10.1002/rmb2.70026","url":null,"abstract":"Background: Embryo quality is a critical determinant of successful outcomes in assisted reproductive technology (ART). Various molecular and cellular mechanisms in oocytes influence embryo quality, and their understanding can lead to the establishment of selection criteria for enhancing implantation rates.Methods: This review summarizes current knowledge on oocyte factors influencing embryo quality, including organelle function, chromosome segregation, maternal transcripts, metabolism, and gene regulation. We also discuss emerging techniques for assessing the fate of embryonic development, such as time-lapse imaging, preimplantation genetic testing for aneuploidy (PGT-A), and artificial intelligence (AI) or machine learning-based prediction models.Main findings: Embryo quality is often determined by maternal factors-driven mechanisms that affect developmental potentials. Advanced technologies such as omics-based profiling and AI-driven analyses offer promising non-invasive assessment tools for embryo quality.Conclusion: Integrating molecular diagnostics of maternal factors with traditional morphological evaluation can refine embryo selection, improving ART success rates. Future research should focus on minimally invasive biomarkers and personalized prediction models.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70026"},"PeriodicalIF":3.3,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12887975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146166606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0