Reproductive Medicine and Biology最新文献_第10页

Combined histological and DNA methylome profiling approaches may provide insights into the pathophysiology of ovarian endometriomas. 将组织学和 DNA 甲基组分析方法结合起来，可以深入了解卵巢子宫内膜异位症的病理生理学。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-12-14 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12548

Ryo Maekawa, Yoshiaki Ota, Ikuko Ota, Yumiko Mihara, Hitomi Takasaki, Shun Sato, Isao Tamura, Yuichiro Shirafuta, Masahiro Shinagawa, Taishi Fujimura, Amon Shiroshita, Toshihide Yoneda, Mai Kawamoto-Jozaki, Fuka Matsui, Toshiaki Taketani, Norihiro Sugino

{"title":"Combined histological and DNA methylome profiling approaches may provide insights into the pathophysiology of ovarian endometriomas.","authors":"Ryo Maekawa, Yoshiaki Ota, Ikuko Ota, Yumiko Mihara, Hitomi Takasaki, Shun Sato, Isao Tamura, Yuichiro Shirafuta, Masahiro Shinagawa, Taishi Fujimura, Amon Shiroshita, Toshihide Yoneda, Mai Kawamoto-Jozaki, Fuka Matsui, Toshiaki Taketani, Norihiro Sugino","doi":"10.1002/rmb2.12548","DOIUrl":"10.1002/rmb2.12548","url":null,"abstract":"Purpose: To test the theory that invaginated ovarian surface epithelium and endometrial implants on the ovary form ovarian endometriomas.Methods: Adhesion sites of ovarian endometrioma on the peritoneum and consecutive ovarian endometrioma cyst wall, called non-adhesion sites, were histologically examined. DNA methylomes of the adhesion sites, non-adhesion sites, and blueberry spots were compared with those of ovary, endometrium, and peritoneum.Results: The non-adhesion sites showed an ovarian surface epithelium-like structure near the adhesion site, which continued to a columnar epithelium-like structure. Calretinin staining was strong in the ovarian surface epithelium-like structure but weak in the columnar epithelium-like structure. Estrogen receptors were absent in the ovarian surface epithelium-like structure, but present in the columnar epithelium-like structure. The adhesion sites had endometrial gland-like structures that expressed estrogen receptors. Analyses of DNA methylomes classified the non-adhesion sites and ovaries into the same group, suggesting that ovarian endometriomas originate from the ovarian surface epithelium. The adhesion sites, blueberry spots and peritoneum were classified in the same group, suggesting that the adhesion sites and blueberry spots originate from the peritoneum.Conclusions: The present results support the invagination theory. Ovarian endometriomas consist of invaginated ovarian surface epithelium with celomic metaplasia and endometrium implants on the peritoneum.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"22 1","pages":"e12548"},"PeriodicalIF":2.7,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138808243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk factors for empty follicle syndrome in assisted reproductive technology with gonadotropin-releasing hormone agonist trigger. 使用促性腺激素释放激素激动剂触发辅助生殖技术的空卵泡综合征的风险因素。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-12-09 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12553

Daichi Inoue, Yoshihiko Sakakibara, Chiharu Ishida, Manami Kondo, Rie Mizuno, Masaya Saito, Shinichi Shibuya, Yoshiki Hashiba, Yoshimasa Asada

{"title":"Risk factors for empty follicle syndrome in assisted reproductive technology with gonadotropin-releasing hormone agonist trigger.","authors":"Daichi Inoue, Yoshihiko Sakakibara, Chiharu Ishida, Manami Kondo, Rie Mizuno, Masaya Saito, Shinichi Shibuya, Yoshiki Hashiba, Yoshimasa Asada","doi":"10.1002/rmb2.12553","DOIUrl":"10.1002/rmb2.12553","url":null,"abstract":"Purpose: To analyze whether response to the GnRH test is a predictor of empty follicle syndrome (EFS) and to analyze independent risk factors for EFS.Methods: The GnRH test results of 3765 patients from 2016 to 2018 were used to define the reference range of the GnRH test. Risk factors for EFS were estimated by multivariate logistic analysis of 5282 cycles (5247 oocyte-retrieved cycles with GnRH agonist trigger and 35 cycles of EFS) conducted from 2016 to 2019.Results: GnRH testing showed basal hormone values as follows: median LH 5.2 (95 percentile; 1.3-12.6) mIU/mL, LH 30 min 22.0 (6.8-57.1), basal FSH 7.3 (3.0-20.5), FSH 30 min 11.5 (5.1-30.4) and FSH/LH ratio 1.5 (0.6-4.1). Independent risk factors for EFS were antral follicle count (adjusted odds ratio; 0.94, 95% CI; 0.89-0.99), basal LH (0.78, 0.66-0.90), and days duration of ovarian stimulation (1.41, 1.21-1. 60). The respective thresholds were 8 for AFC, 5.0 for basal LH, and 16 days for duration.Conclusions: LH 30 min values of the GnRH test did not predict EFS. Independent risk factors for EFS were AFC, basal LH and days duration of ovarian stimulation.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"22 1","pages":"e12553"},"PeriodicalIF":2.7,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10709761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138808253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical outcomes of personalized blastocyst embryo transfer after endometrial receptivity analysis: A multicenter, retrospective cohort study. 子宫内膜容受性分析后个体化囊胚胚胎移植的临床结果:一项多中心、回顾性队列研究。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-11-29 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12550

Yuya Takeshige, Seung Chik Jwa, Yasushi Hirota, Yutaka Osuga, Takeshi Kuramoto, Yasuyuki Mio, Kenji Furui, Masayuki Kinutani, Masahide Shiotani, Yoshimasa Asada, Hirobumi Kamiya, Hiroaki Yoshida, Hideki Igarashi, Koichi Kyono

{"title":"Clinical outcomes of personalized blastocyst embryo transfer after endometrial receptivity analysis: A multicenter, retrospective cohort study.","authors":"Yuya Takeshige, Seung Chik Jwa, Yasushi Hirota, Yutaka Osuga, Takeshi Kuramoto, Yasuyuki Mio, Kenji Furui, Masayuki Kinutani, Masahide Shiotani, Yoshimasa Asada, Hirobumi Kamiya, Hiroaki Yoshida, Hideki Igarashi, Koichi Kyono","doi":"10.1002/rmb2.12550","DOIUrl":"10.1002/rmb2.12550","url":null,"abstract":"Purpose: To evaluate clinical outcomes after endometrial receptivity analysis (ERA).Methods: This was a multicenter, retrospective cohort study involving 861 women who underwent ERA testing at certified fertility clinics in Japan, and who received subsequent personalized blastocyst embryo transfers (ET) between 2018 and 2020. Clinical outcomes, including pregnancies, miscarriages, and live births, were evaluated according to receptivity status for ERA.Results: Mean patient age was 37.7 years (SD = 4.0), and the median number of previous ETs was 2 (interquartile range, 2-3). 41.0% (353/861) of patients were non-receptive for ERA testing. Clinical pregnancy, miscarriage, and live birth rates for personalized blastocyst ET were 44.5% (226/508), 26.1% (59/226), and 26.8% (136/508) for receptive patients, and 43.1% (152/353), 28.3% (43/152), and 28.9% (102/353) for non-receptive patients, all statistically nonsignificant. Multiple logistic regression demonstrated similar nonsignificant associations between receptivity and clinical outcomes. Greater patient age, smoking, and longer duration of infertility were significantly and negatively associated with receptivity, whereas a history of delivery was positively associated and statistically significant.Conclusions: Clinical outcomes after ERA testing were similar between receptive and non-receptive patients. Further prospective study including an appropriate comparison group are warranted to evaluate the efficacy of ERA testing.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"22 1","pages":"e12550"},"PeriodicalIF":2.7,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10685329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138462299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments. 利用荧光化学探针对DNA、微管和微丝进行小鼠胚胎首次裂解的实时成像方法。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-11-27 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12551

Motonari Okabe, Hiromitsu Shirasawa, Yuki Ono, Mayumi Goto, Takuya Iwasawa, Taichi Sakaguchi, Akiko Fujishima, Yohei Onodera, Kenichi Makino, Hiroshi Miura, Yukiyo Kumazawa, Kazumasa Takahashi, Yukihiro Terada

{"title":"An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments.","authors":"Motonari Okabe, Hiromitsu Shirasawa, Yuki Ono, Mayumi Goto, Takuya Iwasawa, Taichi Sakaguchi, Akiko Fujishima, Yohei Onodera, Kenichi Makino, Hiroshi Miura, Yukiyo Kumazawa, Kazumasa Takahashi, Yukihiro Terada","doi":"10.1002/rmb2.12551","DOIUrl":"10.1002/rmb2.12551","url":null,"abstract":"Purpose: Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles and cytoskeleton allows elucidation of the development of early mammalian embryos. The behavior of the DNA and cytoskeleton in early mammalian embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance-expressing gene, into embryos. In this study, we visualized the chronological behavior of male and female chromosome condensation in mouse embryos, beginning in the two-pronuclear zygote, through the first division to the two-cell stage, using fluorescent chemical probes to visualize the behavior of DNA, microtubules, and microfilaments.Method: Mouse two-pronuclear stage embryo were immersed in medium containing fluorescent chemical probes to visualize DNA, microtubules, and microfilaments. Observation was performed with a confocal microscope.Results: This method allowed us to observe how chromosome segregation errors in first somatic cell divisions in mouse embryos and enabled dynamic analysis of a phenomenon called lagging chromosomes.Conclusions: By applying this method, we can observe any stage of embryonic development, which may provide new insights into embryonic development in other mammals.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"22 1","pages":"e12551"},"PeriodicalIF":2.7,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10680128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138462298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-medical oocyte cryopreservation at a single center in Japan: 8 years of experience. 日本单一中心非医学卵母细胞冷冻保存:8年经验。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-11-10 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12549

Koki Yoshinaga, Tomoko Hashimoto, Yuriko Fukuoka, Noriyuki Okuyama, Koichi Kyono

{"title":"Non-medical oocyte cryopreservation at a single center in Japan: 8 years of experience.","authors":"Koki Yoshinaga, Tomoko Hashimoto, Yuriko Fukuoka, Noriyuki Okuyama, Koichi Kyono","doi":"10.1002/rmb2.12549","DOIUrl":"10.1002/rmb2.12549","url":null,"abstract":"Purpose: Increasing numbers of women are opting to undergo non-medical oocyte cryopreservation (NMOC). In this report, we present experience at our clinic and discuss NMOC in Japan.Methods: We followed the progress of 403 women who underwent NMOC at our clinic between 2014 and 2021, totaling 592 reproductive cycles.Results: In total, 61 women underwent oocyte warming and fertility treatment. Of these, 13 women gave birth to 14 children. The median age at first oocyte cryopreservation was 38.3 years, and the oldest pregnant woman was 42 years. Most clients (60%) were in their late 30s. The median time between first oocyte cryopreservation and warming was 3.0 years. One woman was able to achieve a live birth with four vitrified oocytes.Conclusions: This is the first report in Japan documenting pregnancies and childbirths resulting from NMOC. Ideally, women hope to achieve natural pregnancy between 20 and 32 years of age. NMOC is an option for individuals who are unable to pursue pregnancy during optimal reproductive years and wish to preserve their fertility for future attempts. NMOC is recommended in cases with few indications, and it is necessary to continue accumulating data on its long-term safety and effectiveness.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"22 1","pages":"e12549"},"PeriodicalIF":2.7,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10638488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89719391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sperm DNA fragmentation testing in clinical management of reproductive medicine. 生殖医学临床管理中的精子DNA片段检测。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-10-31 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12547

Ava Adler, Bradley Roth, Scott D Lundy, Teppei Takeshima, Yasushi Yumura, Shinnosuke Kuroda

{"title":"Sperm DNA fragmentation testing in clinical management of reproductive medicine.","authors":"Ava Adler, Bradley Roth, Scott D Lundy, Teppei Takeshima, Yasushi Yumura, Shinnosuke Kuroda","doi":"10.1002/rmb2.12547","DOIUrl":"10.1002/rmb2.12547","url":null,"abstract":"Background: Approximately 8%-12% of couples worldwide face infertility, with infertility of individuals assigned male at birth (AMAB) contributing to at least 50% of cases. Conventional semen analysis commonly used to detect sperm abnormalities is insufficient, as 30% of AMAB patients experiencing infertility show normal results in this test. From a genetic perspective, the assessment of sperm DNA fragmentation (SDF) is important as a parameter of sperm quality.Methods: In this narrative study, we review and discuss pathophysiological causes, DNA repair mechanisms, and management of high SDF. We then summarize literature exploring the association between SDF and reproductive outcomes.Main findings: Recent systematic reviews and meta-analyses have revealed a significant association between high SDF in AMAB individuals and adverse reproductive outcomes including embryo development, natural conception, intrauterine insemination, and in vitro fertilization. However, the association with live birth rates and pregnancy rates following intracytoplasmic injection remains inconclusive. The disparities among quantitative assays, inconsistent reference range values, absent high-quality prospective clinical trials, and clinical heterogeneity in AMAB patients with elevated SDF represent the main limitations affecting SDF testing.Conclusion: The evaluation and management of SDF plays an important role in a subset of AMAB infertility, but widespread integration into clinical guidelines will require future high-quality clinical trials and assay standardization.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"22 1","pages":"e12547"},"PeriodicalIF":2.7,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71426369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptozoospermia: Should we use ejaculated sperm or surgically retrieved sperm for assisted reproductive technology? 隐精子症：我们应该使用射出的精子还是手术回收的精子进行辅助生殖技术？

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-10-26 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12546

Hastuki Hibi, Mikiko Tokoro, Megumi Sonohara, Kazuho Ihara, Noritaka Fukunaga, Yoshimasa Asada

{"title":"Cryptozoospermia: Should we use ejaculated sperm or surgically retrieved sperm for assisted reproductive technology?","authors":"Hastuki Hibi, Mikiko Tokoro, Megumi Sonohara, Kazuho Ihara, Noritaka Fukunaga, Yoshimasa Asada","doi":"10.1002/rmb2.12546","DOIUrl":"10.1002/rmb2.12546","url":null,"abstract":"Purpose: In cryptozoospermic subjects, it may often may be difficult to secure motile sperm for assisted reproductive technology (ART). We examined the results of ART with frozen thawed ejaculated sperm in cryptozoospermic subjects and evaluated whether sperm retrieval surgery is necessary for such patients in our clinic.Methods: Between 2013 and 2021, we evaluated 197 cryptozoospermic patients. Age, endocrine panel at the time of the initial semen analysis, and anti-müllerian hormone levels at the time of the spouse's first egg retrieval were examined. Cryopreservation of ejaculated motile sperm collected essentially weekly over a 3-month period was carried out. ART data recorded was the number of egg retrieval cycles, normal fertilization rate, and clinical pregnancy rate.Results: ART using frozen sperm as well as sperm ejaculated on the day of egg retrieval was possible in all cases. The normal fertilization rate was 70.4%, the clinical pregnancy rate per embryo transferred was achieved in 31.5% (870 cycles), and the live birth rate per case was 73.8%.Conclusions: Intracytoplasmic sperm injection (ICSI) was possible without sperm retrieval surgery in cryptozoospermia, resulting in 73.8% of live births per patient. Sperm identification, sperm processing, and ICSI technique are especially important in cryptozoospermia. Sperm retrieval surgery can be avoided in cryptozoospermic patients.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"22 1","pages":"e12546"},"PeriodicalIF":2.7,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10601583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71413758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upregulation of iNOS and phosphorylated eNOS in the implantation-induced blastocysts of mice. iNOS和磷酸化eNOS在植入诱导的小鼠胚泡中的上调。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-10-11 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12545

Misato Seki, Eisaku Takeuchi, Emiko Fukui, Hiromichi Matsumoto

{"title":"Upregulation of iNOS and phosphorylated eNOS in the implantation-induced blastocysts of mice.","authors":"Misato Seki, Eisaku Takeuchi, Emiko Fukui, Hiromichi Matsumoto","doi":"10.1002/rmb2.12545","DOIUrl":"10.1002/rmb2.12545","url":null,"abstract":"Purpose: This study aimed to examine expressions of iNOS and phosphorylated eNOS (p-eNOS) in implantation-induced blastocysts. We also examined the upstream of p-eNOS.Methods: To address the protein expressions in implantation-induced blastocysts, we performed immunohistochemical analysis using a delayed implantation mouse model. Immunostaining for iNOS, p-eNOS, and p-Akt was done. To address the relationship between p-eNOS and p-Akt, activated blastocysts were treated with an Akt inhibitor, MK-2206.Results: iNOS expression was at low levels in dormant blastocysts, whereas the expression was significantly increased in the activated blastocysts. Double staining of p-eNOS and p-Akt in individual blastocysts showed colocalization of p-eNOS and p-Akt of the trophectoderm. p-eNOS and p-Akt expressions were at low levels in dormant blastocysts, whereas both of them were significantly increased in the activated blastocysts. Both dormant and activated blastocysts showed significant positive correlations between p-eNOS and p-Akt. MK-2206 treatment for activated blastocysts showed that blastocysts with lower p-Akt had significantly lower p-eNOS levels.Conclusions: iNOS and p-eNOS, Ca2+ independent NOS, are upregulated by E2 in the blastocysts during implantation activation. Furthermore, p-eNOS is upregulated in implantation-induced blastocysts downstream of p-Akt.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"22 1","pages":"e12545"},"PeriodicalIF":2.7,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2f/97/RMB2-22-e12545.PMC10568119.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41238220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prominin-1 expression in the testis/epididymis and fertility. 促性腺激素-1在睾丸/附睾中的表达和生育能力。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-10-04 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12544

Christine A Fargeas, József Jászai, Denis Corbeil

引用次数: 0

Expression of RSPH6A in the first wave of rat spermatogenesis and oxidative stress conditions: Attenuation by melatonin. RSPH6A在第一波大鼠精子发生和氧化应激条件下的表达：褪黑素的减弱。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-10-02 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12542

Maria Zelinda Romano, Mariem Ben Rhouma, Imed Messaoudi, Francesco Aniello, Sergio Minucci, Massimo Venditti

{"title":"Expression of RSPH6A in the first wave of rat spermatogenesis and oxidative stress conditions: Attenuation by melatonin.","authors":"Maria Zelinda Romano, Mariem Ben Rhouma, Imed Messaoudi, Francesco Aniello, Sergio Minucci, Massimo Venditti","doi":"10.1002/rmb2.12542","DOIUrl":"10.1002/rmb2.12542","url":null,"abstract":"Purpose: Here, we report, for the first time, the temporal expression and localization of axonemal radial spoke head homolog A (RSPH6A) protein during the first wave of rat spermatogenesis and in oxidative stress conditions.Methods: For the developmental study, testes were collected from rats at different developmental stages (7, 14, 21, 28, 35, 42, and 60 postnatal days); for in vivo treatment, 24 rats were treated with cadmium and/or melatonin. From each sample, western blot (WB) and immunofluorescence (IF) analyses for RSPH6A were performed.Results: RSPH6A expression starts at 21 PND alongside the appearance of I spermatocytes (SPC) with a significant increase up to 60 PND. Data were confirmed by IF analysis, showing that RPSH6A expression is restricted to I and II SPC, spermatids, and mature sperm. In vivo experiments showed that the expression and localization of RSPH6A in the testis and epididymal spermatozoa of adult rats treated with cadmium were impaired. Interestingly, melatonin (an antioxidant), given together with Cd, can counteract its damaging effects.Conclusions: All combined data confirm that RSPH6A contributes to the onset of fertility by acting on sperm motility, raising the possibility of using RSPH6A as a marker for normal fertility in the general population.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"22 1","pages":"e12542"},"PeriodicalIF":2.7,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41144750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0