Reproductive Medicine and Biology最新文献_第10页

Preimplantation genetic testing for aneuploidy and chromosomal structural rearrangement: A summary of a nationwide study by the Japan Society of Obstetrics and Gynecology. 非整倍体和染色体结构重排的胚胎植入前基因检测：日本妇产科学会全国性研究摘要。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-05-31 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12518

Takeshi Iwasa, Akira Kuwahara, Toshiyuki Takeshita, Yuka Taniguchi, Mikio Mikami, Minoru Irahara

{"title":"Preimplantation genetic testing for aneuploidy and chromosomal structural rearrangement: A summary of a nationwide study by the Japan Society of Obstetrics and Gynecology.","authors":"Takeshi Iwasa, Akira Kuwahara, Toshiyuki Takeshita, Yuka Taniguchi, Mikio Mikami, Minoru Irahara","doi":"10.1002/rmb2.12518","DOIUrl":"10.1002/rmb2.12518","url":null,"abstract":"Purpose: The Japan Society of Obstetrics and Gynecology conducted a nationwide clinical study to evaluate the pregnancy outcomes of preimplantation genetic testing for aneuploidy or chromosomal structural rearrangement (PGT-A/SR).Methods: Patients that had experienced recurrent implantation failure, recurrent pregnancy loss, or chromosomal structural rearrangement were recruited from 200 fertility centers in Japan. For patients in whom one or more blastocysts were classified as euploid or euploid with suspected mosaicism, a frozen-thawed single embryo transfer (ET) was performed.Results: A total of 10 602 cycles, maternal age 28-50 years, were enrolled in this study. 42 529 blastocysts were biopsied, and 25.5%, 11.7%, and 61.7% of embryos exhibited euploidy, mosaicism, and aneuploidy, respectively. At least one euploid blastocyst was obtained in 38.3% of egg retrieval cycles with embryo biopsy. A total of 6080 ETs were carried out, and the clinical pregnancy rate per ET, ongoing pregnancy rate per ET, and miscarriage rate per pregnancy were 68.8%, 56.3%, and 10.4%, respectively. The rates of clinical pregnancy and miscarriage remained relatively constant across all maternal ages.Conclusions: Preimplantation genetic testing for aneuploidy or chromosomal structural rearrangement may improve the pregnancy rate per ET and reduce the miscarriage rate per pregnancy, especially in patients of advanced maternal age.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10233076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9632132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chlormadinone acetate in progestin-primed ovarian stimulation does not negatively affect clinical results. 醋酸氯地孕酮用于孕激素促排卵不会对临床结果产生负面影响。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-05-31 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12519

Sena Shibasaki, Hiromitsu Hattori, Masae Koizumi, Satoko Nagaura, Mayumi Toya, Hideki Igarashi, Koichi Kyono

{"title":"Chlormadinone acetate in progestin-primed ovarian stimulation does not negatively affect clinical results.","authors":"Sena Shibasaki, Hiromitsu Hattori, Masae Koizumi, Satoko Nagaura, Mayumi Toya, Hideki Igarashi, Koichi Kyono","doi":"10.1002/rmb2.12519","DOIUrl":"10.1002/rmb2.12519","url":null,"abstract":"Purpose: To investigate whether progestin-primed ovarian stimulation (PPOS) with chlormadinone acetate (CMA) adversely affects clinical results and neonatal outcomes, or causes congenital deformities.Methods: This retrospective study was conducted at private IVF clinic from November 2018 to November 2021. Women underwent oocyte retrieval using gonadotropin-releasing hormone (GnRH) antagonist protocol (n = 835) or PPOS protocol (n = 57) were included. Eligible patients were normal ovarian responders (aged <40, AMH ≧1.0 ng/mL) with freeze-all cycle. Embryo developments, clinical results, or neonatal outcomes of singletons derived from transfer of frozen single blastocysts were compared within each group.Results: Patient characteristics were similar in both groups. The median LH level (mIU/mL) at trigger in the GnRH antagonist group [2.0 (1.2-3.7)] was significantly higher than in the PPOS group [0.9 (0.3-1.7)]. There was no cycle with premature LH surge in the PPOS group. Fertilization and blastocyst formation rates did not differ significantly between groups. Furthermore, clinical outcomes were also similar in the two groups. Congenital abnormality rates did not differ significantly [0.9% (3/329), 0.0% (0/17)].Conclusions: CMA using ovarian stimulation did not negatively affect clinical results. Our data suggest that PPOS with CMA is an appropriate ovarian stimulation method for normal ovarian responders.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c3/4d/RMB2-22-e12519.PMC10231652.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9559655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of the follicular and luteal phase lengths and their age-dependent changes in Japanese women: A large population study. 日本女性卵泡期和黄体期长度的分布及其随年龄的变化：一项大型人口研究。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-05-12 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12516

Sawa Mitake, Osamu Wada-Hiraike, Kosuke Kashiwabara, Akari Nakamura, Risa Nasu, Maaya Hine, Yutaka Matsuyama, Tomoyuki Fujii, Yutaka Osuga

{"title":"Distribution of the follicular and luteal phase lengths and their age-dependent changes in Japanese women: A large population study.","authors":"Sawa Mitake, Osamu Wada-Hiraike, Kosuke Kashiwabara, Akari Nakamura, Risa Nasu, Maaya Hine, Yutaka Matsuyama, Tomoyuki Fujii, Yutaka Osuga","doi":"10.1002/rmb2.12516","DOIUrl":"10.1002/rmb2.12516","url":null,"abstract":"Purpose: The current definition of menstrual cycle length in a Japanese woman is different from those of WHO definition, and the original data are outdated. We aimed to calculate the distribution of follicular and luteal phases length in modern Japanese women with various menstrual cycles.Methods: This study determined the lengths of the follicular and luteal phases of Japanese women using basal body temperature data collected via a smartphone application from 2015 to 2019, and the data were analyzed using the Sensiplan method. Over 9 million temperature readings from more than 80 000 participants were analyzed.Results: The mean duration of the low-temperature (follicular) phase averaged 17.1 days and was shorter among participants aged 40-49 years. The mean duration of the high-temperature (luteal) phase was 11.8 days. The variance and maximum-minimum difference of the length of the low temperature period were significant in women under 35 years old than women aged more than 35 years.Conclusions: The shortening of the follicular phase in women aged 40-49 years implied a relationship with the rapid decline of ovarian reserve in these women, and the age 35 years old was turning point of ovulatory function.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/48/f4/RMB2-22-e12516.PMC10182386.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9485911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatty acid supplementation into warming solutions improves pregnancy outcomes after single vitrified-warmed cleavage stage embryo transfers. 在加温溶液中添加脂肪酸可改善单次玻璃化加温卵裂期胚胎移植后的妊娠结局。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-05-08 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12517

Ayumi Amagai, Kenji Ezoe, Tetsuya Miki, Kiyoe Shimazaki, Tadashi Okimura, Keiichi Kato

{"title":"Fatty acid supplementation into warming solutions improves pregnancy outcomes after single vitrified-warmed cleavage stage embryo transfers.","authors":"Ayumi Amagai, Kenji Ezoe, Tetsuya Miki, Kiyoe Shimazaki, Tadashi Okimura, Keiichi Kato","doi":"10.1002/rmb2.12517","DOIUrl":"10.1002/rmb2.12517","url":null,"abstract":"Purpose: This study aimed to examine the embryonic development of human 4-cell stage embryos after warming with fatty acids (FAs) and to assess the pregnancy outcomes after single vitrified-warmed cleavage stage embryo transfers (SVCTs).Methods: Experimental study: A total of 217 discarded, vitrified human 4-cell stage embryos donated for research by consenting couples were used. The embryos were warmed using the fatty acid (FA)-supplemented solutions (FA group) or nonsupplemented solutions (control group). The developmental rate, morphokinetics, and outgrowth competence were analyzed. Clinical study: The treatment records of women undergoing SVCT in natural cycles between April and September 2022 were retrospectively analyzed (April-June 2022, control group; July-September 2022, FA group).Results: Experimental study: The rate of morphologically good blastocysts was significantly higher in the FA group than in the control group (p = 0.0302). The morphokinetics during cleavage, morula, and blastocyst stages were comparable between the groups. The outgrowth was significantly increased in the FA group (p = 0.0438). Clinical study: The rates of implantation, clinical pregnancy, and ongoing pregnancy after SVCTs were significantly increased in the FA group (p = 0.0223-0.0281).Conclusions: Fatty acid-supplemented warming solutions effectively improve embryo development to the blastocyst stage and pregnancy outcomes after SVCTs.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9458158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic variants of G-protein coupled receptors associated with pubertal disorders. 与青春期障碍有关的 G 蛋白偶联受体基因变异。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-04-27 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12515

Erina Suzuki, Mami Miyado, Yoko Kuroki, Maki Fukami

{"title":"Genetic variants of G-protein coupled receptors associated with pubertal disorders.","authors":"Erina Suzuki, Mami Miyado, Yoko Kuroki, Maki Fukami","doi":"10.1002/rmb2.12515","DOIUrl":"10.1002/rmb2.12515","url":null,"abstract":"Background: The human hypothalamic-pituitary-gonadal (HPG) axis is the regulatory center for pubertal development. This axis involves six G-protein coupled receptors (GPCRs) encoded by KISS1R, TACR3, PROKR2, GNRHR, LHCGR, and FSHR.Methods: Previous studies have identified several rare variants of the six GPCR genes in patients with pubertal disorders. In vitro assays and animal studies have provided information on the function of wild-type and variant GPCRs.Main findings: Of the six GPCRs, those encoded by KISS1R and TACR3 are likely to reside at the top of the HPG axis. Several loss-of-function variants in the six genes were shown to cause late/absent puberty. In particular, variants in KISS1R, TACR3, PROKR2, and GNRHR lead to hypogonadotropic hypogonadism in autosomal dominant, recessive, and oligogenic manners. Furthermore, a few gain-of-function variants of KISS1R, PROKR2, and LHCGR have been implicated in precocious puberty. The human HPG axis may contain additional GPCRs.Conclusion: The six GPCRs in the HPG axis govern pubertal development through fine-tuning of hormone secretion. Rare sequence variants in these genes jointly account for a certain percentage of genetic causes of pubertal disorders. Still, much remains to be clarified about the molecular network involving the six GPCRs.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a1/25/RMB2-22-e12515.PMC10134480.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9397594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of experimental techniques for erectile function researches and development of medical technology using animal erectile dysfunction models in sexual and reproductive medicine. 回顾性医学和生殖医学中使用动物勃起功能障碍模型进行勃起功能研究和医疗技术开发的实验技术。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-04-03 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12513

Tomoya Kataoka, Yuji Hotta, Kazunori Kimura

{"title":"A review of experimental techniques for erectile function researches and development of medical technology using animal erectile dysfunction models in sexual and reproductive medicine.","authors":"Tomoya Kataoka, Yuji Hotta, Kazunori Kimura","doi":"10.1002/rmb2.12513","DOIUrl":"10.1002/rmb2.12513","url":null,"abstract":"Background: Erectile dysfunction (ED) is one of the causes of male infertility and is a disease that requires treatment. The first-line drugs for ED are phosphodiesterase 5 (PDE-5) inhibitors, and further treatment options are currently limited. Medical technologies, such as genetic control and regenerative medicine, are developing rapidly. Research on erectile function is progressing rapidly, coupled with technological innovations in other areas.Methods: A PubMed search using the keywords \"animal (rat, mouse, rabbit, dog, and monkey)\" and \"erectile\" was conducted, and all relevant peer-reviewed English results were evaluated.Main findings: The methods for evaluating erectile function include intracavernous pressure (ICP) measurements, isometric tension studies, and dynamic infusion cavernosometry. Papers also reported various disease model animals for the study of diabetes mellitus, cavernous nerve injury, and drug-induced ED.Conclusion: Basic research on ED treatment has progressed rapidly over the past 20 years. In particular, research on the mechanism of ED has been accelerated by the publication of a study on the evaluation of erectile function using ICP measurements in rats. In addition, molecular biological experimental methods such as polymerase chain reaction (PCR) and western blotting have become relatively easy to perform due to technological progress, thus advancing research development.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9f/c9/RMB2-22-e12513.PMC10069627.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9257799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short-term efficacy and safety of early medical abortion in Japan: A multicenter prospective study. 日本早期药物流产的短期疗效和安全性：一项多中心前瞻性研究。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-03-31 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12512

Yutaka Osuga, Kazuhiro Shirasu, Ruriko Tsushima, Ken Ishitani

{"title":"Short-term efficacy and safety of early medical abortion in Japan: A multicenter prospective study.","authors":"Yutaka Osuga, Kazuhiro Shirasu, Ruriko Tsushima, Ken Ishitani","doi":"10.1002/rmb2.12512","DOIUrl":"10.1002/rmb2.12512","url":null,"abstract":"Purpose: To evaluate the short-term efficacy and safety of a combined mifepristone-misoprostol regimen in individuals seeking medical abortion at up to 63 days of gestational age.Methods: This open-label, multicenter, prospective study evaluated the short-term efficacy and safety of medical abortion, with the primary outcome being the abortion success rate 24 h after misoprostol administration. The participants received 200 mg of mifepristone orally and 800 μg of misoprostol buccally in the hospital/clinic 36-48 h later. Bleeding and lower abdominal pain, which are the main symptoms associated with medical abortion, were recorded.Results: The abortion success rate was 93.3% (95% confidence interval [CI]: 87.3-97.1%) within 24 h of misoprostol administration, 63.3% (95% CI: 54.05-71.94%) within 4 h, and 90.0% (95% CI: 83.18-94.73%) within 8 h. The median time from misoprostol administration to a successful abortion was 3.93 h. Bleeding was most commonly observed 0-4 h prior to the confirmation of gestational sac (GS) expulsion. The most intense lower abdominal pain occurred 0-1 h before the confirmation of GS expulsion.Conclusion: The combined regimen of mifepristone and buccal misoprostol for medical abortion showed short-term efficacy and a favorable safety profile.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/52/74/RMB2-22-e12512.PMC10066193.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9602328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The addition of dydrogesterone improves the outcomes of pregnant women with low progesterone levels when receiving vaginal progesterone alone as luteal support in HRT-FET cycles. 在 HRT-FET 周期中，如果仅使用阴道黄体酮作为黄体支持剂，加入地屈孕酮可改善孕酮水平低的孕妇的预后。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-03-22 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12511

Rena Toriumi, Michiharu Horikawa, Chie Sato, Nagisa Shimamura, Rena Ishii, Michiru Terashima, Michiko Hamada, Naoyuki Tachibana, Yuji Taketani

{"title":"The addition of dydrogesterone improves the outcomes of pregnant women with low progesterone levels when receiving vaginal progesterone alone as luteal support in HRT-FET cycles.","authors":"Rena Toriumi, Michiharu Horikawa, Chie Sato, Nagisa Shimamura, Rena Ishii, Michiru Terashima, Michiko Hamada, Naoyuki Tachibana, Yuji Taketani","doi":"10.1002/rmb2.12511","DOIUrl":"10.1002/rmb2.12511","url":null,"abstract":"Purpose: Vaginal progesterone (VP) alone has been used as luteal support (LS) in HRT-FET cycles without measuring serum progesterone concentrations (SPC) because it can achieve adequate intrauterine progesterone levels. However, several reports showed that the co-administration of progestin produced better outcomes than VP alone. We tried to address this discrepancy, focusing on SPC.Methods: VP was given to 180 women undergoing HRT-FET. We measured SPC when pregnancy was diagnosed on day 14 of LS. We compared assisted reproductive technology outcomes between VP alone versus VP + dydrogesterone (D).Results: When using VP alone, average SPC in the miscarriage cases (9.6 ng/mL) were significantly lower compared with the ongoing pregnancy (OP) cases (14.7 ng/mL). The cut-off value for progesterone, 10.7 ng/mL, was a good predictor for the subsequent course of the pregnancy. Of 76 women receiving D ± VP from the start of LS and achieving a pregnancy, the numbers of OP were 44 (84.6%) in SPC ≥ 10.7 ng/mL and 20 (83.3%) in SPC ≤ 10.7 ng/mL with no significant difference.Conclusion: VP alone resulted in lower SPC in some pregnant women in HRT-FET cycles and exhibited a lower OP rate. The co-administration of D improved an OP rate of low progesterone cases to the level comparable with non-low progesterone cases.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10032329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9187802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sirolimus- and cyclosporine-loaded nanostructured lipid carriers: Development, characterization, and in vitro evaluation in T-cell profiles of patients with a history of recurrent pregnancy loss. 含有西罗莫司和环孢素的纳米结构脂质载体：开发、表征和体外评估复发性妊娠失败患者的 T 细胞特征。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-03-20 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12509

Forough Parhizkar, Mehdi Yousefi, Mohammad Sadegh Soltani-Zangbar, Zahra Parhizkar, Leili Aghebati-Maleki, Soheil Abbaspour-Ravasjani, Roza Motavalli, Amin Alizadegan, Maryam Mojahedi, Sina Baharaghdam, Amin Kamrani, Shahla Danaii, Mehdi Talebi, Farhad Jadidi-Niaragh, Hamed Hamishehkar, Hossein Samadi Kafil, Ata Mahmoodpoor, Javad Ahmadian Heris

{"title":"Sirolimus- and cyclosporine-loaded nanostructured lipid carriers: Development, characterization, and in vitro evaluation in T-cell profiles of patients with a history of recurrent pregnancy loss.","authors":"Forough Parhizkar, Mehdi Yousefi, Mohammad Sadegh Soltani-Zangbar, Zahra Parhizkar, Leili Aghebati-Maleki, Soheil Abbaspour-Ravasjani, Roza Motavalli, Amin Alizadegan, Maryam Mojahedi, Sina Baharaghdam, Amin Kamrani, Shahla Danaii, Mehdi Talebi, Farhad Jadidi-Niaragh, Hamed Hamishehkar, Hossein Samadi Kafil, Ata Mahmoodpoor, Javad Ahmadian Heris","doi":"10.1002/rmb2.12509","DOIUrl":"10.1002/rmb2.12509","url":null,"abstract":"Purpose: The authors developed nanostructured lipid carriers (NLCs) loaded with sirolimus (SRL) and cyclosporine (CsA) to improve their therapeutic efficacy in recurrent pregnancy loss (RPL) patients.Methods: Mono-delivery and co-delivery of SRL and CsA by NLCs (S-NLCs, C-NLCs, and S-C-NLCs) were developed. The MTT assay was used to study the optimum dose of formulations. PCR, Western blotting, and ELISA were also conducted.Results: Well-designed nanodrugs with a suitable size, zeta potential, desirable encapsulation efficiency drug loading, and cellular uptake confirmed optimum formulations. Based on cell viability, the amounts of SRL and CsA could be reduced greatly due to the co-delivery by NLCs. Following S-NLCs and C-NLCs interventions in T cells of patients with RPL and immune abnormality, a significant difference was observed in transcription factors and cytokine levels of Th1, Th17, and Tregs compared with healthy samples. Thus, a higher level of pro-inflammatory cytokines (IFN-γ, TNF-α, IL-17, and IL-21) and their regulators (T-bet and RORγt), as well as a lower level of an anti-inflammatory cytokine (IL-10) and its regulatory (Foxp3), were observed. However, no significant difference was found following the S-C-NLCs intervention.Conclusions: S-C-NLCs effectively balance the immune responses in peripheral T cells in RPL patients to induce maternal immune tolerance.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/55/c5/RMB2-22-e12509.PMC10026110.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9518611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of oocyte quality through the SIRT signaling pathway. 通过 SIRT 信号通路改善卵母细胞质量。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-02-23 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12510

Kim Cat Tuyen Vo, Yorino Sato, Kazuhiro Kawamura

引用次数: 0