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Neurotensin induces sustainable activation of the ErbB-ERK1/2 pathway, which is required for developmental competence of oocytes in mice. 神经营养素诱导 ErbB-ERK1/2 通路的持续激活,这是小鼠卵母细胞发育能力所必需的。
IF 2.7 3区 医学
Reproductive Medicine and Biology Pub Date : 2024-03-20 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12571
Asako Okamoto, Tomoya Nakanishi, Shingo Tonai, Masayuki Shimada, Yasuhisa Yamashita
{"title":"Neurotensin induces sustainable activation of the ErbB-ERK1/2 pathway, which is required for developmental competence of oocytes in mice.","authors":"Asako Okamoto, Tomoya Nakanishi, Shingo Tonai, Masayuki Shimada, Yasuhisa Yamashita","doi":"10.1002/rmb2.12571","DOIUrl":"10.1002/rmb2.12571","url":null,"abstract":"<p><strong>Purpose: </strong>LH induces the expression of EGF-like factors and their shedding enzyme (ADAM17) in granulosa cells (GCs), which is essential for ovulation via activation of the ErbB-ERK1/2 pathway in cumulus cells (CCs). Neurotensin (NTS) is reported as a novel regulator of ovulation, whereas the NTS-induced maturation mechanism in oocytes remains unclear. In this study, we focused on the role of NTS in the expression of EGF-like factors and ErbBs, and ADAM17 activity, during oocyte maturation and ovulation in mice.</p><p><strong>Methods: </strong>The expression and localization in GC and CC were examined. Next, hCG and NTS receptor 1 antagonist (SR) were injected into eCG-primed mice, and the effects of SR on ERK1/2 phosphorylation were investigated. Finally, we explored the effects of SR on the expression of EGF-like factors and ErbBs, and ADAM17 activity in GC and CC.</p><p><strong>Results: </strong>NTS was significantly upregulated in GC and CC following hCG injection. SR injection suppressed oocyte maturation and ERK1/2 phosphorylation. SR also downregulated part of the expression of EGF-like factors and their receptors, and ADAM17 activity.</p><p><strong>Conclusions: </strong>NTS induces oocyte maturation through the sustainable activation of the ERK1/2 signaling pathway by upregulating part of the EGF-like factor-induced pathway during oocyte maturation in mice.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12571"},"PeriodicalIF":2.7,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10951886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140176223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The roles of sex hormones in the pathophysiology of age-related sarcopenia and frailty. 性激素在与年龄相关的肌肉疏松症和虚弱的病理生理学中的作用。
IF 2.7 3区 医学
Reproductive Medicine and Biology Pub Date : 2024-03-11 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12569
Tatsuya Hosoi, Mitsutaka Yakabe, Seiji Hashimoto, Masahiro Akishita, Sumito Ogawa
{"title":"The roles of sex hormones in the pathophysiology of age-related sarcopenia and frailty.","authors":"Tatsuya Hosoi, Mitsutaka Yakabe, Seiji Hashimoto, Masahiro Akishita, Sumito Ogawa","doi":"10.1002/rmb2.12569","DOIUrl":"10.1002/rmb2.12569","url":null,"abstract":"<p><strong>Background: </strong>Sarcopenia is an age-related condition characterized by a progressive and systemic decline in skeletal muscle mass, quality, and strength. The incidence of sarcopenia contains sex-specific aspects, indicating the contribution of sex hormones to its pathophysiology. This review focuses on changing trends in sarcopenia, discusses alterations in definitions and diagnostic criteria, and emphasizes the association between sarcopenia and sex hormones.</p><p><strong>Methods: </strong>A literature search was performed on PubMed for related articles published between 1997 and December 2023 using appropriate keywords.</p><p><strong>Main findings results: </strong>Advances in research have emphasized the significance of muscle quality and strength over muscle mass, resulting in new diagnostic criteria for sarcopenia. Androgens demonstrated anabolic effects on skeletal muscles and played a significant role in the pathophysiology of sarcopenia. In clinical settings, androgen replacement therapy has exhibited certain positive outcomes for treating sarcopenia, despite concerns about potential side effects. Conversely, estrogen is involved in skeletal muscle maintenance, but the detailed mechanisms remain unclear. Moreover, results regarding the clinical application of estrogen replacement therapy for treating sarcopenia remained inconsistent.</p><p><strong>Conclusion: </strong>The elucidation of molecular mechanisms that involve sex hormones is eagerly awaited for novel therapeutic interventions for sarcopenia.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12569"},"PeriodicalIF":2.7,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Micromapping testicular sperm extraction: A new technique for microscopic testicular sperm extraction in nonobstructive azoospermia. 显微睾丸取精术:非梗阻性无精子症的显微睾丸取精新技术。
IF 2.7 3区 医学
Reproductive Medicine and Biology Pub Date : 2024-03-11 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12566
Toshihiro Tai, Wakaba Miyamoto, Yuriko Fukuoka, Sena Shibasaki, Mizuho Takahashi, Noriyuki Okuyama, Hiromitsu Hattori, Ikue Ishikawa, Satoko Nagaura, Koki Yoshinaga, Masae Koizumi, Tomoko Hashimoto, Mayumi Toya, Jin Kumagai, Hideki Igarashi, Koichi Kyono
{"title":"Micromapping testicular sperm extraction: A new technique for microscopic testicular sperm extraction in nonobstructive azoospermia.","authors":"Toshihiro Tai, Wakaba Miyamoto, Yuriko Fukuoka, Sena Shibasaki, Mizuho Takahashi, Noriyuki Okuyama, Hiromitsu Hattori, Ikue Ishikawa, Satoko Nagaura, Koki Yoshinaga, Masae Koizumi, Tomoko Hashimoto, Mayumi Toya, Jin Kumagai, Hideki Igarashi, Koichi Kyono","doi":"10.1002/rmb2.12566","DOIUrl":"10.1002/rmb2.12566","url":null,"abstract":"<p><strong>Purpose: </strong>In microscopic testicular sperm extraction (mTESE) for nonobstructive azoospermia (NOA), sperm can be recovered relatively easily in some cases, and mTESE may be retrospectively considered excessive. However, mTESE is routinely performed in the majority of NOA patients because of the difficulty in predicting tissue status. A minimally invasive and comprehensive sperm retrieval method that allows on-the-spot tissue assessment is needed. We have developed and evaluated a novel sperm retrieval technique for NOA called micromapping testicular sperm extraction (MMTSE).</p><p><strong>Methods: </strong>MMTSE involves dividing the testis into four sections and making multiple small needle holes in the tunica albuginea to extract seminiferous tubules and retrieve sperm. The sperm-positive group by MMTSE (Group I) underwent additional tissue collection (ATC) via a small incision, whereas the sperm-negative group by MMTSE (Group 0) underwent mTESE.</p><p><strong>Results: </strong>In total, 40 NOA participants underwent MMTSE. Group I included 15 patients and Group 0 included 25 patients. In Group 1, sperm were recovered from all patients by ATC. In Group 0, sperm were recovered in 4 of 25 cases using mTESE.</p><p><strong>Conclusions: </strong>MMTSE shows promise as a simple method that comprehensively searches testicular tissue and retrieves sperm using an appropriate method while minimizing patient burden.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12566"},"PeriodicalIF":2.7,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolomic and microbiome analysis of cervicovaginal mucus in in vitro fertilization-embryo transfer: Toward predicting pregnancy success. 体外受精-胚胎移植中宫颈阴道粘液的代谢组和微生物组分析:预测妊娠成功率。
IF 2.7 3区 医学
Reproductive Medicine and Biology Pub Date : 2024-03-11 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12568
Eiji Nishio, Aya Iwata, Rie Kawasaki, Kukimoto Iwao, Haruki Nishizawa, Takuma Fujii
{"title":"Metabolomic and microbiome analysis of cervicovaginal mucus in in vitro fertilization-embryo transfer: Toward predicting pregnancy success.","authors":"Eiji Nishio, Aya Iwata, Rie Kawasaki, Kukimoto Iwao, Haruki Nishizawa, Takuma Fujii","doi":"10.1002/rmb2.12568","DOIUrl":"10.1002/rmb2.12568","url":null,"abstract":"<p><strong>Purpose: </strong>In the context of in vitro fertilization-embryo transfer (IVF-ET), factors other than egg quality may be key determinants of treatment success, in particular, maternal factors related to uterine endometrial receptivity and unidentified factors. We therefore aimed to analyze the metabolome and microbiome in IVF-ET patients who did and did not achieve pregnancy.</p><p><strong>Methods: </strong>Cervicovaginal mucus was collected from patients undergoing IVF-ET. Metabolite analysis was conducted by liquid chromatography-mass spectrometry and the microbiota were determined by the polymerase chain reaction using universal 16S-rRNA gene bacterial primers by MiSeq sequencing. Patients were classified as pregnant (<i>N</i> = 10) or nonpregnant (<i>N</i> = 13). Metabolic pathways were examined by MetaboAnalyst.</p><p><strong>Results: </strong>Three metabolic pathways, including alanine-aspartate-glutamate metabolism, arginine biosynthesis, and cysteine-methionine metabolism, were commonly decreased at the time of embryo transfer irrespective pregnant outcomes. Notably, pyruvate was decreased in the pregnant group. Amino acid metabolites showed inverse correlations with the presence of anaerobic microbiota in the nonpregnant group.</p><p><strong>Conclusions: </strong>Metabolism decreased during embryo transplantation, with a notable decrease in pyruvate metabolism, particularly in patients who became pregnant. The behavior of metabolites in the pregnant and nonpregnant groups suggests that metabolome analysis in the cervicovaginal mucus may be a diagnostic marker for predicting pregnancy.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12568"},"PeriodicalIF":2.7,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The number of previous implantation failures is a critical determinant of intrauterine autologous platelet-rich plasma infusion success in women with recurrent implantation failure. 对于反复种植失败的妇女来说,之前种植失败的次数是决定宫内自体富血小板血浆输注成功与否的关键因素。
IF 2.7 3区 医学
Reproductive Medicine and Biology Pub Date : 2024-02-29 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12565
Shunsaku Fujii, Takaaki Oguchi
{"title":"The number of previous implantation failures is a critical determinant of intrauterine autologous platelet-rich plasma infusion success in women with recurrent implantation failure.","authors":"Shunsaku Fujii, Takaaki Oguchi","doi":"10.1002/rmb2.12565","DOIUrl":"10.1002/rmb2.12565","url":null,"abstract":"<p><strong>Purpose: </strong>We aimed to identify factors influencing the reproductive outcomes of frozen-thawed embryo transfer (FET) with intrauterine autologous platelet-rich plasma (PRP) infusion in patients with either a thin endometrium or recurrent implantation failure (RIF) despite a normal endometrial appearance.</p><p><strong>Methods: </strong>In this retrospective study of women who underwent PRP-FET, factors influencing PRP-FET outcomes were identified using multivariate logistic regression analysis.</p><p><strong>Results: </strong>We enrolled 111 patients (70 with refractory thin endometrium and 41 with RIF but no thin endometrium). For 99 completed FET cycles, the β-hCG positivity rate was 46.7%, clinical pregnancy rate (CPR) was 41.0%, and live birth rate (LBR) was 36.2%. PRP treatment was associated with significant improvements over previous cycles, and participants with thin endometria demonstrated thickening. Multivariate logistic regression analysis showed that the number of previous implantation failures in women with RIF was a significant factor affecting the PRP-FET outcomes. The CPR and LBR of women with RIF were lower when there had been ≥3 previous implantation failures occurred.</p><p><strong>Conclusions: </strong>Intrauterine PRP infusion improves the pregnancy outcomes of patients with RIF or a thin endometrium. The number of previous implantation failures is a critical determinant of successful intrauterine PRP infusions in women with RIF.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12565"},"PeriodicalIF":2.7,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10904764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140022537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of cell-cell interaction between mural granulosa cells and cumulus granulosa cells during ovulation using single-cell RNA sequencing data of mouse ovary. 利用小鼠卵巢单细胞 RNA 测序数据分析排卵过程中壁粒细胞和积粒细胞之间的细胞间相互作用。
IF 2.7 3区 医学
Reproductive Medicine and Biology Pub Date : 2024-02-14 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12564
Yuichiro Shirafuta, Isao Tamura, Amon Shiroshita, Taishi Fujimura, Ryo Maekawa, Toshiaki Taketani, Norihiro Sugino
{"title":"Analysis of cell-cell interaction between mural granulosa cells and cumulus granulosa cells during ovulation using single-cell RNA sequencing data of mouse ovary.","authors":"Yuichiro Shirafuta, Isao Tamura, Amon Shiroshita, Taishi Fujimura, Ryo Maekawa, Toshiaki Taketani, Norihiro Sugino","doi":"10.1002/rmb2.12564","DOIUrl":"10.1002/rmb2.12564","url":null,"abstract":"<p><strong>Purpose: </strong>We investigated the interactions between mural granulosa cells (MGCs) and cumulus granulosa cells (CGCs) during ovulation after the LH surge.</p><p><strong>Methods: </strong>We performed clustering, pseudotime, and interactome analyses utilizing reported single-cell RNA sequencing data of mouse ovary at 6 h after eCG-hCG injection.</p><p><strong>Results: </strong>Clustering analysis classified granulosa cells into two distinct populations, MGCs and CGCs. Pseudotime analysis divided granulosa cells into before and after the LH surge, and further divided them into two branches, the ovulatory MGCs and the ovulatory CGCs. Interactome analysis was performed to identify the interactions between MGCs and CGCs. Twenty-six interactions were acting from CGCs toward MGCs, involving ovulation and steroidogenesis. Thirty-six interactions were acting from MGCs toward CGCs, involving hyaluronan synthesis. There were 25 bidirectional interactions, involving the EGFR pathway. In addition, we found three novel interactions: Ephrins-Ephs pathway and Wnt-Lrp6 pathway from CGCs to MGCs, associated with steroidogenesis and lipid transport, respectively, and TGF-β-TGFBR1 pathway from MGCs to CGCs, associated with hyaluronan synthesis.</p><p><strong>Conclusions: </strong>MGCs and CGCs interact with each other in the preovulatory follicle after the LH surge, and their interactions have roles in corpus luteum formation, oocyte maturation, and follicle rupture.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12564"},"PeriodicalIF":2.7,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10867398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139741881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reinfusion of peritoneal fluid elevates the level of plasma D-dimer in patients with early-onset ovarian hyperstimulation syndrome. 再输注腹腔液会使早发卵巢过度刺激综合征患者的血浆D-二聚体水平升高。
IF 2.7 3区 医学
Reproductive Medicine and Biology Pub Date : 2024-02-14 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12563
Shiori Kumazawa, Kazuki Saito, Nanako Hashido, Rinko Ibi, Tomonori Ishikawa, Akira Wakabayashi, Naoyuki Miyasaka
{"title":"Reinfusion of peritoneal fluid elevates the level of plasma D-dimer in patients with early-onset ovarian hyperstimulation syndrome.","authors":"Shiori Kumazawa, Kazuki Saito, Nanako Hashido, Rinko Ibi, Tomonori Ishikawa, Akira Wakabayashi, Naoyuki Miyasaka","doi":"10.1002/rmb2.12563","DOIUrl":"10.1002/rmb2.12563","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to elucidate the factors that affect the dynamics of blood D-dimer in ovarian hyperstimulation syndrome (OHSS).</p><p><strong>Methods: </strong>We retrospectively reviewed medical records from two hospitals and extracted data obtained during assisted reproductive technology and OHSS treatment. Blood D-dimer levels during hospitalization were plotted against body weight. Other factors possibly related to blood D-dimer levels were also analyzed.</p><p><strong>Results: </strong>The analysis included 10 patients with OHSS admitted between January 2013 and June 2023. In all patients, blood D-dimer levels increased significantly when they convalesced from OHSS and lost weight. None of the patients showed clinical signs of thrombosis, which was confirmed using imaging tests in 8 of 10 patients. Two patients underwent cell-free and concentrated ascites reinfusion therapy (CART), and their blood D-dimer levels increased dramatically after the procedure.</p><p><strong>Conclusion: </strong>Weight change and CART are associated with blood D-dimer dynamics in OHSS. Our results show that elevated blood D-dimer levels in patients with OHSS do not always represent the presence of thrombosis. Reinfusion of pooled D-dimer in ascites may explain the D-dimer surge during the recovery phase or after CART in these patients. Our study provides new perspectives on the clinical implications of D-dimer during OHSS.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12563"},"PeriodicalIF":2.7,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10867380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139741882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Swim-up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing. 在精子处理过程中,泳动法比密度梯度离心法更能保持精子 DNA 的完整性。
IF 2.7 3区 医学
Reproductive Medicine and Biology Pub Date : 2024-01-29 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12562
Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao
{"title":"Swim-up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing.","authors":"Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao","doi":"10.1002/rmb2.12562","DOIUrl":"10.1002/rmb2.12562","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to evaluate the effects of swim-up and density gradient centrifugation methods on sperm DNA fragmentation.</p><p><strong>Methods: </strong>Nineteen normozoospermic patient samples with ≥100 × 10<sup>6</sup> motile sperms were included in this study. Sperm DNA fragmentation, progressive motility, and progressive motile sperm number were measured before and after the swim-up method or density gradient centrifugation.</p><p><strong>Results: </strong>Sperm DNA fragmentation was not statistically different between swim-up-(14.4 ± 2.1%, <i>p</i> = 0.32) and density gradient centrifugation-processed (25.0 ± 3.0%, <i>p</i> = 0.20) and unprocessed semen samples (19.2 ± 1.9%). Sperm DNA fragmentation was significantly lower in swim-up-than in density gradient centrifugation-processed samples (<i>p</i> < 0.05). Sperm progressive motility was significantly higher (<i>p</i> < 0.05) in swim-up-(92.9 ± 1.0%) and density gradient centrifugation-processed (81.3 ± 2.0%) samples, with the former being higher, than in unprocessed semen samples (53.1 ± 3.7%). The recovery rate of progressive motile sperms was significantly lower in swim-up-(9.7 ± 1.4%) than in density gradient centrifugation-processed samples (17.2 ± 1.8%, <i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>The swim-up method is superior to density gradient centrifugation, evidenced by less sperm DNA fragmentation and higher sperm progressive motility. The recovery rate of progressive motile sperms was better after density gradient centrifugation than after swim-up.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12562"},"PeriodicalIF":2.7,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10823384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139576632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reviewer Acknowledgement. 感谢审稿人。
IF 3.4 3区 医学
Reproductive Medicine and Biology Pub Date : 2024-01-20 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12555
{"title":"Reviewer Acknowledgement.","authors":"","doi":"10.1002/rmb2.12555","DOIUrl":"https://doi.org/10.1002/rmb2.12555","url":null,"abstract":"","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12555"},"PeriodicalIF":3.4,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10799256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139513519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
microRNAs associated with the quality of follicular fluids affect oocyte and early embryonic development. 与卵泡液质量有关的 microRNA 会影响卵母细胞和早期胚胎发育。
IF 2.7 3区 医学
Reproductive Medicine and Biology Pub Date : 2024-01-18 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12559
Sogo Aoki, Yuki Inoue, Shunsuke Hara, Jun Itou, Koumei Shirasuna, Hisataka Iwata
{"title":"microRNAs associated with the quality of follicular fluids affect oocyte and early embryonic development.","authors":"Sogo Aoki, Yuki Inoue, Shunsuke Hara, Jun Itou, Koumei Shirasuna, Hisataka Iwata","doi":"10.1002/rmb2.12559","DOIUrl":"10.1002/rmb2.12559","url":null,"abstract":"<p><strong>Purpose: </strong>Oocyte and embryo quality differs significantly among individuals. Follicular fluid (FF) is a solo environment of oocyte maturation and may flux into the oviduct. Supplementation of in vitro maturation (IVM) and culture (IVC) medium with extracellular vesicles of FFs supports oocyte maturation and embryonic development. We addressed a hypothesis that miRNA profiles in FFs are crucial background of oocyte maturation and embryonic development.</p><p><strong>Methods: </strong>FFs were collected from the ovaries of individual cows, and the FFs were classified into Good or Poor FF based on the developmental rate to the blastocyst stage of enclosed oocytes. miRNAs associated with the Good FFs were explored using small RNA sequencing. In addition, FFs were classified using the concentration of Good-FF-associated miRNAs. These classified FFs or miRNA were added to the IVM or IVC mediums.</p><p><strong>Results: </strong>Supplementation of IVM and IVC medium with Good FF improved embryonic development. Good FFs contained miR-151-3p and miR-425-5p at a high concentration compared with those in Poor FFs. FFs selected by the concentration of miR-151-3p and miR-425-5p improved oocyte maturation and embryonic development. Supplementation of IVM or IVC medium with either miR-151-3p or miR-425-5p improved embryonic development to the blastocyst stage.</p><p><strong>Conclusion: </strong>miRNAs were associated with the Good FFs determined oocyte maturation and embryonic development.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12559"},"PeriodicalIF":2.7,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10795439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139492067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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