Reproductive Medicine and Biology最新文献_第9页

Analysis of cell-cell interaction between mural granulosa cells and cumulus granulosa cells during ovulation using single-cell RNA sequencing data of mouse ovary. 利用小鼠卵巢单细胞 RNA 测序数据分析排卵过程中壁粒细胞和积粒细胞之间的细胞间相互作用。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-02-14 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12564

Yuichiro Shirafuta, Isao Tamura, Amon Shiroshita, Taishi Fujimura, Ryo Maekawa, Toshiaki Taketani, Norihiro Sugino

{"title":"Analysis of cell-cell interaction between mural granulosa cells and cumulus granulosa cells during ovulation using single-cell RNA sequencing data of mouse ovary.","authors":"Yuichiro Shirafuta, Isao Tamura, Amon Shiroshita, Taishi Fujimura, Ryo Maekawa, Toshiaki Taketani, Norihiro Sugino","doi":"10.1002/rmb2.12564","DOIUrl":"10.1002/rmb2.12564","url":null,"abstract":"Purpose: We investigated the interactions between mural granulosa cells (MGCs) and cumulus granulosa cells (CGCs) during ovulation after the LH surge.Methods: We performed clustering, pseudotime, and interactome analyses utilizing reported single-cell RNA sequencing data of mouse ovary at 6 h after eCG-hCG injection.Results: Clustering analysis classified granulosa cells into two distinct populations, MGCs and CGCs. Pseudotime analysis divided granulosa cells into before and after the LH surge, and further divided them into two branches, the ovulatory MGCs and the ovulatory CGCs. Interactome analysis was performed to identify the interactions between MGCs and CGCs. Twenty-six interactions were acting from CGCs toward MGCs, involving ovulation and steroidogenesis. Thirty-six interactions were acting from MGCs toward CGCs, involving hyaluronan synthesis. There were 25 bidirectional interactions, involving the EGFR pathway. In addition, we found three novel interactions: Ephrins-Ephs pathway and Wnt-Lrp6 pathway from CGCs to MGCs, associated with steroidogenesis and lipid transport, respectively, and TGF-β-TGFBR1 pathway from MGCs to CGCs, associated with hyaluronan synthesis.Conclusions: MGCs and CGCs interact with each other in the preovulatory follicle after the LH surge, and their interactions have roles in corpus luteum formation, oocyte maturation, and follicle rupture.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12564"},"PeriodicalIF":2.7,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10867398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139741881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reinfusion of peritoneal fluid elevates the level of plasma D-dimer in patients with early-onset ovarian hyperstimulation syndrome. 再输注腹腔液会使早发卵巢过度刺激综合征患者的血浆D-二聚体水平升高。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-02-14 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12563

Shiori Kumazawa, Kazuki Saito, Nanako Hashido, Rinko Ibi, Tomonori Ishikawa, Akira Wakabayashi, Naoyuki Miyasaka

{"title":"Reinfusion of peritoneal fluid elevates the level of plasma D-dimer in patients with early-onset ovarian hyperstimulation syndrome.","authors":"Shiori Kumazawa, Kazuki Saito, Nanako Hashido, Rinko Ibi, Tomonori Ishikawa, Akira Wakabayashi, Naoyuki Miyasaka","doi":"10.1002/rmb2.12563","DOIUrl":"10.1002/rmb2.12563","url":null,"abstract":"Purpose: This study aimed to elucidate the factors that affect the dynamics of blood D-dimer in ovarian hyperstimulation syndrome (OHSS).Methods: We retrospectively reviewed medical records from two hospitals and extracted data obtained during assisted reproductive technology and OHSS treatment. Blood D-dimer levels during hospitalization were plotted against body weight. Other factors possibly related to blood D-dimer levels were also analyzed.Results: The analysis included 10 patients with OHSS admitted between January 2013 and June 2023. In all patients, blood D-dimer levels increased significantly when they convalesced from OHSS and lost weight. None of the patients showed clinical signs of thrombosis, which was confirmed using imaging tests in 8 of 10 patients. Two patients underwent cell-free and concentrated ascites reinfusion therapy (CART), and their blood D-dimer levels increased dramatically after the procedure.Conclusion: Weight change and CART are associated with blood D-dimer dynamics in OHSS. Our results show that elevated blood D-dimer levels in patients with OHSS do not always represent the presence of thrombosis. Reinfusion of pooled D-dimer in ascites may explain the D-dimer surge during the recovery phase or after CART in these patients. Our study provides new perspectives on the clinical implications of D-dimer during OHSS.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12563"},"PeriodicalIF":2.7,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10867380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139741882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Swim-up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing. 在精子处理过程中，泳动法比密度梯度离心法更能保持精子 DNA 的完整性。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-01-29 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12562

Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao

{"title":"Swim-up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing.","authors":"Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao","doi":"10.1002/rmb2.12562","DOIUrl":"10.1002/rmb2.12562","url":null,"abstract":"Purpose: This study aimed to evaluate the effects of swim-up and density gradient centrifugation methods on sperm DNA fragmentation.Methods: Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study. Sperm DNA fragmentation, progressive motility, and progressive motile sperm number were measured before and after the swim-up method or density gradient centrifugation.Results: Sperm DNA fragmentation was not statistically different between swim-up-(14.4 ± 2.1%, p = 0.32) and density gradient centrifugation-processed (25.0 ± 3.0%, p = 0.20) and unprocessed semen samples (19.2 ± 1.9%). Sperm DNA fragmentation was significantly lower in swim-up-than in density gradient centrifugation-processed samples (p < 0.05). Sperm progressive motility was significantly higher (p < 0.05) in swim-up-(92.9 ± 1.0%) and density gradient centrifugation-processed (81.3 ± 2.0%) samples, with the former being higher, than in unprocessed semen samples (53.1 ± 3.7%). The recovery rate of progressive motile sperms was significantly lower in swim-up-(9.7 ± 1.4%) than in density gradient centrifugation-processed samples (17.2 ± 1.8%, p < 0.05).Conclusions: The swim-up method is superior to density gradient centrifugation, evidenced by less sperm DNA fragmentation and higher sperm progressive motility. The recovery rate of progressive motile sperms was better after density gradient centrifugation than after swim-up.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12562"},"PeriodicalIF":2.7,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10823384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139576632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reviewer Acknowledgement. 感谢审稿人。

IF 3.4 3区医学

Reproductive Medicine and Biology Pub Date : 2024-01-20 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12555

引用次数: 0

microRNAs associated with the quality of follicular fluids affect oocyte and early embryonic development. 与卵泡液质量有关的 microRNA 会影响卵母细胞和早期胚胎发育。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-01-18 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12559

Sogo Aoki, Yuki Inoue, Shunsuke Hara, Jun Itou, Koumei Shirasuna, Hisataka Iwata

{"title":"microRNAs associated with the quality of follicular fluids affect oocyte and early embryonic development.","authors":"Sogo Aoki, Yuki Inoue, Shunsuke Hara, Jun Itou, Koumei Shirasuna, Hisataka Iwata","doi":"10.1002/rmb2.12559","DOIUrl":"10.1002/rmb2.12559","url":null,"abstract":"Purpose: Oocyte and embryo quality differs significantly among individuals. Follicular fluid (FF) is a solo environment of oocyte maturation and may flux into the oviduct. Supplementation of in vitro maturation (IVM) and culture (IVC) medium with extracellular vesicles of FFs supports oocyte maturation and embryonic development. We addressed a hypothesis that miRNA profiles in FFs are crucial background of oocyte maturation and embryonic development.Methods: FFs were collected from the ovaries of individual cows, and the FFs were classified into Good or Poor FF based on the developmental rate to the blastocyst stage of enclosed oocytes. miRNAs associated with the Good FFs were explored using small RNA sequencing. In addition, FFs were classified using the concentration of Good-FF-associated miRNAs. These classified FFs or miRNA were added to the IVM or IVC mediums.Results: Supplementation of IVM and IVC medium with Good FF improved embryonic development. Good FFs contained miR-151-3p and miR-425-5p at a high concentration compared with those in Poor FFs. FFs selected by the concentration of miR-151-3p and miR-425-5p improved oocyte maturation and embryonic development. Supplementation of IVM or IVC medium with either miR-151-3p or miR-425-5p improved embryonic development to the blastocyst stage.Conclusion: miRNAs were associated with the Good FFs determined oocyte maturation and embryonic development.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12559"},"PeriodicalIF":2.7,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10795439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139492067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond appearance: Can morphologically low-grade euploid blastocysts yield successful pregnancies? 超越外表：形态学上低级别的优倍囊胚能否成功妊娠？

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-01-18 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12560

Takahiro Suzuki, Chiharu Ishida, Yoko Yoshioka, Masae Kojima, Mikiko Tokoro, Noritaka Fukunaga, Yoshimasa Asada

{"title":"Beyond appearance: Can morphologically low-grade euploid blastocysts yield successful pregnancies?","authors":"Takahiro Suzuki, Chiharu Ishida, Yoko Yoshioka, Masae Kojima, Mikiko Tokoro, Noritaka Fukunaga, Yoshimasa Asada","doi":"10.1002/rmb2.12560","DOIUrl":"10.1002/rmb2.12560","url":null,"abstract":"Purpose: The primary objective of this investigation is to evaluate how morphological quality affects the pregnancy outcomes in euploid embryos determined by preimplantation genetic testing for aneuploidies (PGT-A). Concurrently, as a secondary objective, we aim to identify which specific aspects of morphological evaluation exert the most significant impact on these outcomes.Methods: A retrospective analysis of 451 single euploid embryo transfer cycles at our clinic was conducted. Embryos were evaluated based on the degree of blastocyst expansion, inner cell mass (ICM), trophectoderm (TE) morphology, and the day of blastocyst vitrification. Outcomes between morphologically low-grade and high-grade embryos were compared. Additionally, the study analyzed which morphological factors most influenced pregnancy outcomes.Results: Pregnancy outcomes were significantly lower in morphologically low-grade blastocysts compared to high-grade ones. Among the morphological evaluations, the ICM assessment was significantly associated with the live birth rate.Conclusion: Our study indicates that the morphological quality of euploid embryos, particularly the evaluation of the ICM, plays a crucial role in IVF-ET success.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12560"},"PeriodicalIF":2.7,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10797159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139513516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of the optimization of thyroid function, thrombophilia, immunity and uterine milieu treatment strategy for recurrent implantation failure and recurrent pregnancy loss. 复发性着床失败和复发性妊娠失败的甲状腺功能、血栓性疾病、免疫和子宫环境优化治疗策略综述。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-01-18 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12561

Keiji Kuroda

{"title":"A review of the optimization of thyroid function, thrombophilia, immunity and uterine milieu treatment strategy for recurrent implantation failure and recurrent pregnancy loss.","authors":"Keiji Kuroda","doi":"10.1002/rmb2.12561","DOIUrl":"10.1002/rmb2.12561","url":null,"abstract":"Background: Aside from embryonic factors, various factors can intricately interfere with embryo implantation and maintenance of pregnancy, causing recurrent implantation failure (RIF) or recurrent pregnancy loss (RPL). This review focuses the optimization of thyroid function, thrombophilia, immunity, and uterine milieu (OPTIMUM) treatment strategy on RIF and RPL.Methods: Three studies employing the OPTIMUM treatment strategy for patients with RIF and/or RPL were reviewed.Results: The OPTIMUM improved pregnancy rates in women with RIF aged <40 years. Among advanced age women, however, no significant differences in pregnancy rates were observed between the control, OPTIMUM, and preimplantation genetic testing for aneuploidy (PGT-A) groups, although pregnancy rates were highest after OPTIMUM + PGT-A. The OPTIMUM reduced miscarriage rates in women with RPL aged <40 years. Among advanced age women, PGT-A, but not the OPTIMUM, contributed to miscarriage prevention. Factors predicting pregnancy success in women with RIF who received the OPTIMUM included thrombophilia and young age. Risk factors for an unsuccessful live birth among women with RPL who received the OPTIMUM included advanced age, infertility, diminished ovarian reserve, and non-ART treatment.Conclusions: The OPTIMUM can improve pregnancy outcomes in women with RIF/RPL, except for advanced age women with embryonic factor-induced reproductive failure.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12561"},"PeriodicalIF":2.7,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10797160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139513512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tacrolimus treatment in women with repeated implantation failures. 他克莫司治疗反复植入失败的妇女。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-01-09 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12558

Koji Nakagawa, Rikikazu Sugiyama

{"title":"Tacrolimus treatment in women with repeated implantation failures.","authors":"Koji Nakagawa, Rikikazu Sugiyama","doi":"10.1002/rmb2.12558","DOIUrl":"10.1002/rmb2.12558","url":null,"abstract":"Background: Tacrolimus is an immunosuppressive drug that works as a calcineurin inhibitor to improve the reproductive outcomes for women who have experienced multiple implantation failures (RIF) and show elevated type 1 helper T (Th1)/Th2 cell ratios.Methods: In the first part of this review, we indicate how we re-evaluated the cut-off index for selecting the participants in a tacrolimus regimen via transferred euploid blastocysts. In the second part, we cite cases where tacrolimus has improved the live birth rate for women who have experienced recurrent pregnancy losses (PRL) and we introduce the utility of tacrolimus treatment to prevent obstetrical complications.Main findings: After reconsideration of the cut-off index (Th1/Th2 ≥ 11.8), however, the pregnancy rates of women with tacrolimus were significantly higher than those of women without tacrolimus. The PRL women treated with tacrolimus showed significantly lower rates of biochemical pregnancy, but higher live-birth rates compared with women who were not treated with tacrolimus. Moreover, prior severe obstetrical complications could be controlled via the administration of tacrolimus during pregnancy.Conclusion: Tacrolimus has become indispensable in the field of solid-organ transplantation, and in the near future, it should become an essential agent in the reproductive field, as well.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12558"},"PeriodicalIF":2.7,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10775185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assisted reproductive technology in Japan: A summary report for 2021 by the Ethics Committee of the Japan Society of Obstetrics and Gynecology. 日本的辅助生殖技术：日本妇产科学会伦理委员会 2021 年总结报告。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-12-30 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12552

Yukiko Katagiri, Seung Chik Jwa, Akira Kuwahara, Takeshi Iwasa, Masanori On, Keiichi Kato, Hiroshi Kishi, Yoshimitsu Kuwabara, Fuminori Taniguchi, Miyuki Harada, Akira Iwase, Yutaka Osuga

{"title":"Assisted reproductive technology in Japan: A summary report for 2021 by the Ethics Committee of the Japan Society of Obstetrics and Gynecology.","authors":"Yukiko Katagiri, Seung Chik Jwa, Akira Kuwahara, Takeshi Iwasa, Masanori On, Keiichi Kato, Hiroshi Kishi, Yoshimitsu Kuwabara, Fuminori Taniguchi, Miyuki Harada, Akira Iwase, Yutaka Osuga","doi":"10.1002/rmb2.12552","DOIUrl":"10.1002/rmb2.12552","url":null,"abstract":"Purpose: The Japan Society of Obstetrics and Gynecology (JSOG) registry gathers comprehensive data from registered assisted reproductive technology (ART) facilities in Japan. Herein, we report 2021 ART cycle characteristics and outcomes.Methods: Descriptive statistics were used to summarize and analyze 2021 data.Results: In 2021, 625 ART facilities participated in the registry; 27 facilities did not conduct ART cycles and 598 registered treatment cycles. In total, 498 140 cycles were registered, and there were 69 797 neonates (increases of 10.7% and 15.5%, respectively, from the previous year). The number of freeze-all in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles decreased in 2021; the number of neonates born was 2268 for IVF-embryo transfer (ET) cycles and 2850 for ICSI cycles. Frozen-thawed ET (FET) cycles increased markedly from 2020 (11.2% increase). In 2021, 239 428 FET cycles were conducted, resulting in 87 174 pregnancies and 64 679 neonates. For fresh transfers, the total single ET, singleton pregnancy rate, and singleton live birth rates were 82.7%, 97.0%, and 97.3%; for FET, these rates were 84.9%, 96.9%, and 97.1%.Conclusions: The 2021 Japanese ART registry analysis showed marked increases in both total treatment cycles and live births from the previous year.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12552"},"PeriodicalIF":2.7,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10757097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139074883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic efficacy of the optimization of thyroid function, thrombophilia, immunity and uterine milieu (OPTIMUM) treatment strategy on pregnancy outcomes after single euploid blastocyst transfer in advanced age women with recurrent reproductive failure. 优化甲状腺功能、血栓性疾病、免疫和子宫环境（OPTIMUM）治疗策略对反复生殖失败的高龄产妇单倍体囊胚移植后妊娠结局的疗效。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2023-12-22 eCollection Date: 2023-01-01 DOI: 10.1002/rmb2.12554

Keiji Kuroda, Takashi Horikawa, Azusa Moriyama, Yuko Ojiro, Satoru Takamizawa, Hideaki Watanabe, Tetsuo Maruyama, Shuko Nojiri, Koji Nakagawa, Rikikazu Sugiyama

{"title":"Therapeutic efficacy of the optimization of thyroid function, thrombophilia, immunity and uterine milieu (OPTIMUM) treatment strategy on pregnancy outcomes after single euploid blastocyst transfer in advanced age women with recurrent reproductive failure.","authors":"Keiji Kuroda, Takashi Horikawa, Azusa Moriyama, Yuko Ojiro, Satoru Takamizawa, Hideaki Watanabe, Tetsuo Maruyama, Shuko Nojiri, Koji Nakagawa, Rikikazu Sugiyama","doi":"10.1002/rmb2.12554","DOIUrl":"10.1002/rmb2.12554","url":null,"abstract":"Purpose: To clarify the efficacy of the OPtimization of Thyroid function, Thrombophilia, IMmunity and Uterine Milieu (OPTIMUM) treatment strategy on pregnancy outcomes after euploid blastocyst transfer in advanced age women with recurrent implantation failure (RIF) and/or recurrent pregnancy loss (RPL).Methods: Between January 2019 and May 2022, 193 consecutive women aged ≥40 years with RIF and/or RPL received single euploid blastocyst transfer. Before embryo transfer, 127 women underwent RIF/RPL testing. Chronic endometritis was treated with mainly antibiotics, aberrant high Th1/Th2 cell ratios with vitamin D and/or tacrolimus, overt/subclinical hypothyroidism with levothyroxine, and thrombophilia with low-dose aspirin. We compared pregnancy outcomes in the women who did and did not receive the OPTIMUM treatment strategy.Results: Women with RIF/RPL in the OPTIMUM group had significantly higher clinical pregnancy and livebirth rates than did those in the control group (clinical pregnancy rate of 71.7% and 45.5%, p < 0.001; livebirth rate of 64.6% and 39.4%, p = 0.001, respectively). However, preimplantation genetic testing for aneuploidy with and without OPTIMUM promoted low miscarriage rates with no significant difference between them (9.9%, and 13.3%, respectively; p = 0.73).Conclusions: The OPTIMUM treatment strategy improved clinical pregnancy rates after single euploid blastocyst transfer; but not miscarriage rates.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"22 1","pages":"e12554"},"PeriodicalIF":2.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10739138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0