{"title":"KIF2C Deletion Causes Meiotic Abnormalities and Nonobstructive Azoospermia in Mice.","authors":"Hiroaki Kitakaze, Haruhiko Miyata, Yuki Oyama, Chen Pan, Yuma Kujime, Go Tsujimura, Takahiro Imanaka, Sohei Kuribayashi, Norichika Ueda, Kentaro Takezawa, Shinichiro Fukuhara, Norio Nonomura, Masahito Ikawa","doi":"10.1002/rmb2.12659","DOIUrl":"10.1002/rmb2.12659","url":null,"abstract":"<p><strong>Purpose: </strong>Kinesin Family Member 2C (KIF2C) is a key regulator of microtubule dynamics and chromosome segregation in mitosis. However, its role in spermatogenesis remains unclear. Recent transcriptomic analyses suggest a potential link between KIF2C and male infertility. This study aimed to clarify KIF2C's roles in spermatogenesis using <i>Kif2c</i> knockout (KO) mice.</p><p><strong>Methods: </strong>To overcome the preweaning lethality associated with <i>Kif2c</i> deletion, we generated <i>Kif2c</i> KO mice with a mixed genetic background of 129X1/SvJ and B6D2. We assessed male fertility, epididymal sperm counts, and testicular sections of <i>Kif2c</i> KO mice.</p><p><strong>Results: </strong>Global <i>Kif2c</i> KO mice were obtained and showed male infertility. Histological analyses and epididymal sperm count revealed that <i>Kif2c</i> KO mice exhibited severely impaired spermatogenesis and absence of mature spermatozoa. These findings are consistent with those observed in patients with nonobstructive azoospermia (NOA). Our classification of <i>Kif2c</i> KO seminiferous tubules indicated that most spermatogenic cells were arrested at the early stages, particularly during meiosis.</p><p><strong>Conclusions: </strong>This study provides in vivo evidence that KIF2C is essential for spermatogenesis and male fertility in mice. The successful generation of global <i>Kif2c</i> KO mice establishes an animal model for NOA, supporting research on germ cell development and reproductive health.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12659"},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12107603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wanqi Chen, Qi Feng, Chan Zhang, Lu Yang, Jingyi Qi
{"title":"Exosomes From Human Umbilical Cord Mesenchymal Stem Cells Alleviate Oxidative Stress-Induced POI by Regulating Autophagic Homeostasis Through the AMPK Pathway.","authors":"Wanqi Chen, Qi Feng, Chan Zhang, Lu Yang, Jingyi Qi","doi":"10.1002/rmb2.12658","DOIUrl":"10.1002/rmb2.12658","url":null,"abstract":"<p><strong>Background: </strong>Increased oxidative stress is a key factor in developing premature ovarian insufficiency (POI). Exosome therapy emerges as a promising cell-free treatment. However, research into the molecular mechanisms of exosome repair in ovarian diseases is still in its infancy. By establishing models of oxidative stress in ovarian granulosa cells and POI in mice, we aim to explore whether human umbilical cord mesenchymal stem cell exosomes can repair oxidative damage in ovarian granulosa cells and mouse ovaries, as well as identify potential targets of action. Our goal is to provide new ideas and methods for the clinical application of exosomes and the early prevention and treatment of POI.</p><p><strong>Methods: </strong>Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) (200 μM, 2 h) and D-galactose (D-gal) (200 mg/kg, 56 days) were used to induce oxidative stress in ovarian granulosa cells and mice, respectively. Subsequently, exosomes were added to the injury model to validate the mechanism of exosome repair of oxidative damage. We evaluated senescence indicators, AMPK activation, and autophagy.</p><p><strong>Result: </strong>Through the execution of in vivo and in vitro experiments, it was observed that the activation of the AMP-activated protein kinase (AMPK) pathway is induced by exosome intervention, leading to a reduction in the accumulation of autophagic vesicles and the restoration of the patency of autophagic flow. This, in turn, results in the repair of oxidative stress-induced damage and the enhancement of the function of damaged cells and ovaries.</p><p><strong>Conclusion: </strong>Our findings indicate that exosomes derived from human umbilical cord stem cells have the beneficial effect of ameliorating oxidative stress-induced POI by activating AMPK and regulating autophagic homeostasis.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12658"},"PeriodicalIF":2.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12106360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Foundational basic animal experiments paved the way for the clinical development of uterus transplantation.","authors":"Iori Kisu, Makoto Mihara, Hisako Hara, Yojiro Kato, Yusuke Matoba, Yohei Yamada, Kentaro Matsubara, Hideaki Obara, Nobuhiko Suganuma, Kouji Banno","doi":"10.1002/rmb2.12656","DOIUrl":"10.1002/rmb2.12656","url":null,"abstract":"<p><p>Uterus transplantation (UTx) has emerged as a revolutionary treatment for absolute uterine factor infertility, made possible by extensive preclinical research. Animal studies have played a pivotal role in advancing UTx to clinical practice. We highlight Japan's contribution, including non-human primate research and regulatory progress, leading to its anticipated clinical implementation. The expansion of UTx in the Asia-Oceania region underscores its global impact. Further refinements in surgical techniques, optimization of immunosuppressive regimens, and establishment of clear patient eligibility criteria will be crucial for ensuring the long-term success and sustainability of UTx programs worldwide. This letter acknowledges the Swedish team's foundational efforts in establishing UTx as a viable reproductive technology.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12656"},"PeriodicalIF":2.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12093328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of aging on semen and embryonic developmental scores in assisted reproductive technology.","authors":"Taiyo Yamamoto, Katsuya Mine, Hisataka Iwata","doi":"10.1002/rmb2.12647","DOIUrl":"10.1002/rmb2.12647","url":null,"abstract":"<p><strong>Purpose: </strong>The effects of female aging on fertility have been extensively studied; however, this is not the case for aging males. Embryonic selection using time-lapse observations is helpful for successful embryo transfer; however, information on the effect of male aging on time-lapse is insufficient. We analyzed the impact of paternal aging on sperm characteristics, embryonic developmental kinetics, embryo evaluation score, and pregnancy outcomes.</p><p><strong>Methods: </strong>We used data from patients treated at our clinic between January 2020 and December 2022. We evaluated the effects of aging in men and women on semen data, in vitro fertilization (IVF) results, developmental kinetics, embryo evaluation scores, and embryo transfer outcomes using a retrospective approach.</p><p><strong>Results: </strong>Male aging adversely affected the semen characteristics. Although female aging had adverse effects on IVF, embryonic developmental kinetics, and embryo transfer outcomes, male aging did not have such a significant impact. Female aging decreased the iDAScore and Gardner criteria, whereas male aging did not affect the iDAScore.</p><p><strong>Conclusions: </strong>Aging in males had a negative effect on semen data. Contrary to the impact of aging on women, aging in men did not have a significant effect on embryo and gestation rates following embryo transfer.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12647"},"PeriodicalIF":2.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of embryo cryopreservation before surgery on clinical outcomes in IVF patients with endometrioma.","authors":"Nozomi Takahashi, Yuma Kawahara, Miyuki Harada, Tomoko Makabe, Gentaro Izumi, Osamu Wada-Hiraike, Yasushi Hirota, Yutaka Osuga","doi":"10.1002/rmb2.12654","DOIUrl":"10.1002/rmb2.12654","url":null,"abstract":"<p><strong>Purpose: </strong>This study evaluated whether embryo cryopreservation before surgery (ECBS) improves clinical outcomes in in vitro fertilization (IVF) patients with endometrioma.</p><p><strong>Methods: </strong>This retrospective study included patients aged 28-42 years with endometrioma who underwent oocyte retrieval at our hospital from 2019 to 2022. Seventeen patients who underwent ECBS and 43 patients who underwent embryo transfer (ET) without surgery were included. Patient characteristics, reproductive outcomes, and obstetric outcomes were compared between the groups.</p><p><strong>Results: </strong>Maximum cyst size was significantly larger in the ECBS group than in the control group. The abortion rate per pregnancy was significantly lower (0% vs. 35.5%) in the ECBS group than in the control group. The ongoing pregnancy rate per case was significantly higher in the ECBS group than in the control group (88.2% vs. 58.1%), while the time to ongoing pregnancy was similar. Among patients in the ECBS group who experienced live births, 84.6% became pregnant following three or fewer ET attempts. Multivariate analysis revealed that ECBS was the only factor associated with ongoing pregnancy. The rates of perinatal complications are comparable between the groups.</p><p><strong>Conclusions: </strong>ECBS is an effective method to improve reproductive outcomes in IVF patients with endometrioma without prolonging the time to pregnancy.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12654"},"PeriodicalIF":2.7,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12056301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The quality of human eggs and its pre-IVF incubation.","authors":"Ri-Cheng Chian, Yi-Chun Guan, Xiao-Jin He, Jian Xu, Jin-Hui Shu, Jian-Hua Li","doi":"10.1002/rmb2.12652","DOIUrl":"10.1002/rmb2.12652","url":null,"abstract":"<p><strong>Background: </strong>Multi-factors influence the success rate of infertility treatments, and one of the important points is to obtain good quality eggs.</p><p><strong>Methods: </strong>Based on the literatures and unpublished data, the factors affecting egg quality were summarized.</p><p><strong>Main findings results: </strong>Egg quality is an important determinant in successful infertility treatment. In addition to maternal age, controlled ovarian hyperstimulation (COH) protocols also play a key role in affecting the quality of the egg. After egg retrieval, the insemination occurs 3-6 h after collection, with a pre-IVF incubation time by in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) (39-42 h post-HCG injection). The pre-IVF incubation refers to the short period time of 3 to 6 h after oocyte retrieval and before the insemination by IVF or ICSI. The pre-IVF incubation of collected eggs in the designed culture medium improves egg quality in terms of maturation and early embryonic development.</p><p><strong>Conclusions: </strong>Pre-IVF incubation of the collected eggs contributes to the improvement of the quality of eggs; therefore, it may increase subsequent pregnancy and implantation rates following embryo transfer.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12652"},"PeriodicalIF":2.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative prospective study on the clinical utility of G-banding and next-generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan.","authors":"Hidemine Honda, Tsuyoshi Takiuchi, Mika Handa, Nao Wakui, Saori Tsuji, Takeshi Goto, Shota Suzuki, Fumie Saji, Tatsuya Miyake, Sakae Goto, Satomi Okamura, Tomomi Yamada, Michiko Kodama, Tadashi Kimura","doi":"10.1002/rmb2.12655","DOIUrl":"10.1002/rmb2.12655","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the clinical utility of G-banding and next-generation sequencing (NGS) for chromosomal analysis of products of conception (POC), a crucial tool for detecting fetal chromosomal abnormalities which are major causes of miscarriage and stillbirth.</p><p><strong>Methods: </strong>We evaluated the clinical utility of both techniques in a prospective analysis of 40 patients who experienced miscarriages or stillbirths between 6 and 36 weeks of gestation under Advanced Medical Care A in Japan. Both methods were applied to the same POC samples. The primary outcome was the proportion of patients with a presumed cause of miscarriage or stillbirth among all submitted samples.</p><p><strong>Results: </strong>NGS presumed the cause in 75.0% (30/40) of cases, significantly outperforming G-banding's 42.5% (17/40) (<i>p</i> < 0.01). G-banding could analyze 67.5% (27/40) of the samples owing to culture failure, whereas NGS successfully analyzed all samples (100%, 40/40) (<i>p</i> < 0.01). Among the successfully analyzed samples, NGS presumed the cause in 70.3% (19/27) of cases, compared with 62.9% (17/27) for G-banding (<i>p</i> = 0.31). For miscarriages before 12 weeks, NGS presumed the cause in 73.5% (25/34) of cases, significantly higher than the 44.1% (15/34) (<i>p</i> < 0.01) presumed using G-banding.</p><p><strong>Conclusions: </strong>These results highlight the superior efficacy of NGS over G-banding for presuming causes of miscarriage or stillbirth.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12655"},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fan Dong, Zhong Zheng, Ying Ding, Yi Ma, Si-Qi Wang, Xiang-Feng Chen, Ping Ping
{"title":"Preimplantation genetic testing might not be the necessity for male patients with 47,XYY syndrome: A pilot study.","authors":"Fan Dong, Zhong Zheng, Ying Ding, Yi Ma, Si-Qi Wang, Xiang-Feng Chen, Ping Ping","doi":"10.1002/rmb2.12650","DOIUrl":"10.1002/rmb2.12650","url":null,"abstract":"<p><strong>Purpose: </strong>This pilot study aimed to explore the necessity for 47,XYY syndrome males (couples) to perform PGT rather than conventional In Vitro Fertilization (IVF)/Intracytoplasmic Sperm Injection (ICSI) cycles.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted with 36 nonmosaic and mosaic 47,XYY syndrome patients (couples) undergoing 43 oocyte retrieval cycles (37 planned for PGT and 6 for IVF/ICSI) between December 2017 and December 2023. The couples were given either next-generation sequencing-based PGT or conventional IVF/ICSI followed by 45 embryo transfer (ET) cycles (38 from PGT and 7 from IVF/ICSI). The detailed cytogenetic results of the 129 embryos from PGT were analyzed, and the pregnancy and neonatal outcomes between PGT-ET and conventional IVF/ICSI-ET cycles were compared.</p><p><strong>Results: </strong>The PGT results showed that the chance of sex chromosome abnormalities was low (1.55%), with chromosomal errors being observed more often in autosomes. Importantly, no differences were observed in the rates of biochemical pregnancy, implantation, clinical pregnancy, ongoing pregnancy, pregnancy loss, live birth, and preterm delivery between PGT-ET cycles and conventional IVF/ICSI-ET cycles. Comparable results regarding gestational age, birthweight, low birthweight rate, macrosomia rate, male rate, as well as the rate of congenital anomalies were also observed between the two groups.</p><p><strong>Conclusions: </strong>Preimplantation genetic testing might not be necessary to conduct for 47,XYY syndrome males unless there are other indications. Studies with large populations are in demand to confirm the present results.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12650"},"PeriodicalIF":2.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12012309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimizing treatment efficacy and fertility preservation in patients undergoing hematopoietic stem cell transplantation: A narrative review of ovarian shielding with total-body irradiation or treosulfan-based conditioning regimens.","authors":"Yuji Tanaka, Tetsuro Hanada, Tsukuru Amano, Akimasa Takahashi, Mari Deguchi, Hiroyuki Yamanaka, Shunichiro Tsuji, Takashi Murakami","doi":"10.1002/rmb2.12648","DOIUrl":"10.1002/rmb2.12648","url":null,"abstract":"<p><strong>Background: </strong>Pediatric and adolescent/young adult (AYA) patients with hematologic malignancies often require hematopoietic stem cell transplantation (HSCT) using conditioning regimens that pose high risks for gonadal toxicity. Traditional protocols, including total body irradiation (TBI) and busulfan-based regimens, can impair fertility. This review explores the potential of gonadal shielding during TBI and treosulfan-based conditioning as strategies to optimize treatment efficacy while preserving fertility.</p><p><strong>Methods: </strong>A PubMed search up to February 2025 was performed for English, peer-reviewed articles on hematologic malignancies, HSCT, shielding, and treosulfan. Studies on oncologic outcomes and fertility in pediatric and AYA patients were included.</p><p><strong>Main findings: </strong>Ovarian shielding during myeloablative conditioning with TBI effectively reduces ovarian radiation exposure, resulting in improved menstrual recovery and hormone profiles. A treosulfan-based regimen demonstrated higher antitumor activity than a reduced-intensity busulfan-based regimen in randomized controlled trials. In a retrospective analysis, the treosulfan-based regimen exhibited lower gonadal toxicity than the busulfan-based regimen, although careful attention must be paid to dosing settings of the regimens.</p><p><strong>Conclusion: </strong>Ovarian shielding during TBI and a treosulfan-based regimen hold the potential to preserve the reproductive capacity of patients undergoing HSCT. Future clinical studies that appropriately assess both oncological outcomes and fertility are needed to validate these findings.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12648"},"PeriodicalIF":2.7,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144046227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A machine learning model for predicting fertilization following short-term insemination using embryo images.","authors":"Masato Saito, Hirofumi Haraguchi, Ikumi Nakajima, Shinya Fukuda, Chenghua Zhu, Norio Masuya, Kazunori Matsumoto, Yuya Yoshikawa, Tomoki Tanaka, Satoshi Kishigami, Leona Matsumoto","doi":"10.1002/rmb2.12649","DOIUrl":"10.1002/rmb2.12649","url":null,"abstract":"<p><strong>Purpose: </strong>This study established a machine learning model (MLM) trained on embryo images to predict fertilization following short-term insemination for early rescue ICSI and compared its predictive performance with the embryologist's manual classification.</p><p><strong>Methods: </strong>Embryo images at 4.5 and 8 h post-insemination were preprocessed into vectors using ResNet50. The Light Gradient Boosting Machine (Light GBM) was employed for training vectors. Fertilization in the test dataset was assessed by MLM, with seven senior and 11 junior embryologists. Predictive metrics were analyzed using repeated measures ANOVA and paired <i>t</i>-tests.</p><p><strong>Results: </strong>Comparing MLM, senior embryologists, and junior embryologists, significant differences were observed in accuracy (0.71 ± 0.01, 0.75 ± 0.05, 0.61 ± 0.05), recall (0.84 ± 0.02, 0.84 ± 0.10, 0.61 ± 0.07), F1-score (0.78 ± 0.01, 0.81 ± 0.04, 0.66 ± 0.04), and area under the curve (0.73 ± 0.0 3, 0.73 ± 0.06, 0.61 ± 0.07), the MLM outperforming junior embryologists with <1 year of experience. No significant differences were observed between the MLM and senior embryologists with over 5 years of experience.</p><p><strong>Conclusions: </strong>MLM can effectively predict fertilization following short-term insemination by analyzing cytoplasmic changes in images. These results underscore the potential to enhance clinical decision-making and improve patient outcomes.</p>","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12649"},"PeriodicalIF":2.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}