Reproductive Medicine and Biology最新文献

Emergency Fertility Preservation in Hematological Diseases: Ovarian Response and Real-World Outcomes From China. 血液病的紧急生育能力保存：卵巢反应和来自中国的现实世界结果。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-05-04 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70057

Jinghua Zhang, Fang Fang, Cheng Cheng, Jianliu Wang, Lanping Xu, Li Tian

{"title":"Emergency Fertility Preservation in Hematological Diseases: Ovarian Response and Real-World Outcomes From China.","authors":"Jinghua Zhang, Fang Fang, Cheng Cheng, Jianliu Wang, Lanping Xu, Li Tian","doi":"10.1002/rmb2.70057","DOIUrl":"https://doi.org/10.1002/rmb2.70057","url":null,"abstract":"Purpose: This study investigated ovarian response, safety, and reproductive outcomes in patients with hematological diseases undergoing controlled ovarian stimulation (COS) for emergency fertility preservation (FP).Methods: We retrospectively analyzed female patients with hematological diseases receiving COS for emergency FP at our center from January 2016 to February 2025. Outcomes were compared by menstrual cycle phase at stimulation initiation, protocols, and chemotherapy exposure, with follow-up data on hematologic disease status, utilization of cryopreserved oocytes/embryos, ovarian function, marital, and reproductive collected.Results: No statistically significant differences in ovarian response were detected according to the phase of the menstrual cycle at stimulation initiation or between the gonadotropin-releasing hormone antagonist (GnRH-ant) and progestin-primed ovarian stimulation (PPOS) protocols. Chemotherapy exposure within 3 months was associated with lower oocyte and mature oocyte yield. Most patients underwent hematopoietic stem cell transplantation (HSCT), and 92.31% developed premature ovarian insufficiency (POI). Six patients underwent warming of oocytes/embryos, achieving two live births including one twin.Conclusions: During emergency FP in patients with hematological diseases, random-start COS using either GnRH-ant or PPOS protocols yields comparable outcomes, supporting its feasibility and flexible application in time-constrained settings. Recent chemotherapy exposure may adversely affect COS outcomes and should be considered when planning treatment.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70057"},"PeriodicalIF":3.3,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13137434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the Follicular Fluid Microenvironment: Integrative Biomarkers and Multiomics Signatures to Predict IVF Outcomes. 解码卵泡液微环境：综合生物标志物和多组学特征预测试管婴儿结果

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-05-04 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70056

Ayako Muraoka, Hiroaki Kajiyama

{"title":"Decoding the Follicular Fluid Microenvironment: Integrative Biomarkers and Multiomics Signatures to Predict IVF Outcomes.","authors":"Ayako Muraoka, Hiroaki Kajiyama","doi":"10.1002/rmb2.70056","DOIUrl":"https://doi.org/10.1002/rmb2.70056","url":null,"abstract":"Background: With the increase in the number of patients with infertility, the number of patients requiring in vitro fertilization and embryo transfer (IVF-ET) is also increasing. No established method exists for predicting pregnancy rates under IVF-ET, and the urgent development of pregnancy predictive biomarkers is required. Follicular fluid (FF) can be collected noninvasively during oocyte retrieval and contains numerous substances indicative of follicular status, making it a strong candidate for such biomarkers.Methods: This review summarizes the predictive potential of several biomarkers in FF for pregnancy. We focused on FF components such as hormones, proteins, growth factors, inflammatory markers, oxidative stress markers, metabolomics, and nucleic acids.Main findings: FF contains stem cell factors, interleukin-6, coenzyme Q10, vitamin D, cell-free deoxyribonucleic acid, and micro ribonucleic acids within extracellular vesicles, which can predict in vitro fertilization (IVF) success. Recently, omics has enabled the analysis of a wide range of constituents, contributing to the search for potential biomarkers.Conclusion: This review presents a comprehensive overview of relevant FF biomarkers for predicting IVF outcomes. Reliable FF predictive biomarkers are expected to improve future IVF success rates and embryo selection, thereby enhancing overall assisted reproductive technology outcomes.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70056"},"PeriodicalIF":3.3,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13137436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "Expression and Function of Meflin in Human Endometrial Decidualization". 更正“Meflin在人子宫内膜去个体化中的表达和功能”。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-04-26 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70054

引用次数: 0

Functional Impairment of Activated Regulatory T Cells as a Central Immunological Defect in Endometriosis. 活化调节性T细胞功能损伤作为子宫内膜异位症的中心免疫缺陷。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-04-16 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70051

Yukiko Tanaka, Eiko Maeda, Hiroyuki Okimura, Maya Fujii, Yosuke Tarumi, Masahide Hamaguchi, Fukui Michiaki, Khaleque N Khan, Jo Kitawaki, Taisuke Mori

{"title":"Functional Impairment of Activated Regulatory T Cells as a Central Immunological Defect in Endometriosis.","authors":"Yukiko Tanaka, Eiko Maeda, Hiroyuki Okimura, Maya Fujii, Yosuke Tarumi, Masahide Hamaguchi, Fukui Michiaki, Khaleque N Khan, Jo Kitawaki, Taisuke Mori","doi":"10.1002/rmb2.70051","DOIUrl":"https://doi.org/10.1002/rmb2.70051","url":null,"abstract":"Background: Endometriosis is increasingly recognized as a disorder of impaired reproductive immune tolerance rather than solely an endocrine disease. Although previous studies have reported inconsistent findings regarding the number of regulatory T cells (Tregs), the functional integrity of distinct Treg subsets and their contribution to disease pathogenesis remains unclear.Methods: We reviewed clinical, experimental, and translational studies focusing on regulatory T-cell biology in endometriosis, with emphasis on functional Treg subsets, immune interactions within ectopic lesions, and emerging immunomodulatory therapeutic approaches.Main findings: Accumulating evidence indicates that endometriosis is characterized not by a reduction in total Foxp3+ Treg numbers but by a qualitative deficiency of activated regulatory T cells (aTregs). Local loss of aTregs in ectopic endometrium and ovarian endometrioma is associated with enhanced inflammatory and angiogenic activation, including increased IL-6, CCL2, and VEGF production, macrophage activation, and dysregulated Th17 responses. Experimental studies using Treg-depleted mouse models further demonstrate that impaired Treg-mediated regulation accelerates lesion growth, whereas adoptive Treg transfer suppresses inflammation and disease progression.Conclusion: Functional impairment of activated regulatory T cells represents a central immunological defect underlying chronic inflammation, lesion persistence, and infertility in endometriosis. Restoration of Treg-mediated immune regulation may provide a promising nonhormonal, fertility-preserving therapeutic strategy.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70051"},"PeriodicalIF":3.3,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13087425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autoimmune Thyroid Disease and Female Fertility: Clinical Evidence on Ovarian Reserve, Infertility Treatment Outcomes, and Pathophysiology. 自身免疫性甲状腺疾病与女性生育：卵巢储备、不孕症治疗结果和病理生理学的临床证据。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-04-15 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70052

Akira Iwase, Yuko Hasegawa, Yoshikazu Kitahara, Seung Chik Jwa, Hiromitsu Shirasawa, Michio Kitajima, Yukiko Katagiri, Harada Miyuki, Kawamura Kazuhiro, Satoko Osuka

{"title":"Autoimmune Thyroid Disease and Female Fertility: Clinical Evidence on Ovarian Reserve, Infertility Treatment Outcomes, and Pathophysiology.","authors":"Akira Iwase, Yuko Hasegawa, Yoshikazu Kitahara, Seung Chik Jwa, Hiromitsu Shirasawa, Michio Kitajima, Yukiko Katagiri, Harada Miyuki, Kawamura Kazuhiro, Satoko Osuka","doi":"10.1002/rmb2.70052","DOIUrl":"10.1002/rmb2.70052","url":null,"abstract":"Background: Thyroid autoimmunity (TAI) is prevalent among women of reproductive age and has been associated with impaired fertility; however, its effects on ovarian reserve and infertility treatment outcomes remain controversial.Methods: A narrative review was performed using PubMed to identify clinical and experimental studies addressing TAI, ovarian reserve, premature ovarian insufficiency (POI), and infertility treatment outcomes.Results: Clinical evidence suggests that TAI is associated with modestly reduced AMH levels in adult women, although the magnitude of this decline appears insufficient to indicate imminent POI. In contrast, adolescents with TAI may exhibit elevated AMH. Observational studies describe a frequent coexistence of TAI and POI; however, Mendelian randomization analyses suggest that POI may predispose to TAI rather than the reverse. Mechanistic studies show that thyroid hormones act directly on ovarian cells via thyroid hormone receptors, while immune-mediated pathways involving cytokines, oxidative stress, and anti-ovarian antibodies may contribute to follicular dysfunction. Evidence regarding assisted reproductive technology outcomes in euthyroid TAI remains inconsistent.Conclusion: TAI may exert a subtle influence on ovarian reserve through complex endocrine and immune mechanisms, with age-specific variation. Although AMH alterations are observed, their clinical relevance for fertility and treatment outcomes remains unclear, highlighting the need for longitudinal studies.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70052"},"PeriodicalIF":3.3,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13083598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147722613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Letrozole Reduces Ovulatory Responsiveness in In Vitro-Grown Mouse Follicles. 来曲唑降低体外培养小鼠卵泡的排卵反应性。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-04-15 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70047

Tatsuya Kobayashi, Hiroshi Ishikawa, Hirokazu Tanaka, Kumiko Ishii, Akira Mitsuhashi, Hirokazu Usui, Makio Shozu, Kaori Koga

{"title":"Letrozole Reduces Ovulatory Responsiveness in In Vitro-Grown Mouse Follicles.","authors":"Tatsuya Kobayashi, Hiroshi Ishikawa, Hirokazu Tanaka, Kumiko Ishii, Akira Mitsuhashi, Hirokazu Usui, Makio Shozu, Kaori Koga","doi":"10.1002/rmb2.70047","DOIUrl":"https://doi.org/10.1002/rmb2.70047","url":null,"abstract":"Purpose: This study aimed to determine whether continuous aromatase inhibition with letrozole reduces peri-ovulatory responsiveness to ovulatory stimulation in in vitro-grown mouse follicles.Methods: Early antral follicles were isolated from 21 to 24-day-old C57BL/6jjcl mice and cultured individually for 5 days with letrozole (0.01, 0.1, or 1 μM) or vehicle. Follicle survival and growth were assessed by measuring follicle diameter. Ovulatory responsiveness was evaluated by hCG/EGF stimulation. Expression of ovulation-related genes (Lhcgr, Ptgs2, and Runx1) and a luteinization-related gene (Foxo1) was analyzed using RT-qPCR. The effect of estradiol supplementation was also examined.Results: Letrozole did not affect follicle survival or growth. However, ovulation rates were reduced in a dose-dependent manner (p < 0.0001). At 0.1 μM, letrozole significantly decreased mRNA levels of Lhcgr, Runx1, and Ptgs2 compared with vehicle-treated follicles. Estradiol supplementation restored the expression of these genes and partially rescued ovulatory capacity. In contrast, Foxo1 expression increased in letrozole-treated follicles and was attenuated by estradiol.Conclusions: Continuous letrozole exposure reduces ovulatory responsiveness in in vitro-grown mouse follicles and is associated with decreased Lhcgr expression after hCG stimulation, likely due to estrogen deficiency. These findings suggest that prolonged aromatase inhibition during follicular growth may impair acquisition of peri-ovulatory competence.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70047"},"PeriodicalIF":3.3,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13081686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uterine Endometrial Microbiome and Chronic Endometritis in Relation to Anti-β2-Glycoprotein I (β2GPI)/Human Leukocyte Antigen (HLA)-DR Autoantibodies in Women With Recurrent Implantation Failure and Recurrent Pregnancy Loss: A Cross-Sectional Study. 子宫内膜微生物组和慢性子宫内膜炎与抗β2-糖蛋白I (β2GPI)/人白细胞抗原(HLA)-DR自身抗体在反复植入失败和反复妊娠丢失妇女中的关系：一项横断面研究

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-04-09 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70049

Yosuke Ono, Hajime Ota, Maki Ogi, Yoshiyuki Fukushi, Shinichiro Wada, Hisashi Arase, Osamu Yoshino, Hideto Yamada

{"title":"Uterine Endometrial Microbiome and Chronic Endometritis in Relation to Anti-β2-Glycoprotein I (β2GPI)/Human Leukocyte Antigen (HLA)-DR Autoantibodies in Women With Recurrent Implantation Failure and Recurrent Pregnancy Loss: A Cross-Sectional Study.","authors":"Yosuke Ono, Hajime Ota, Maki Ogi, Yoshiyuki Fukushi, Shinichiro Wada, Hisashi Arase, Osamu Yoshino, Hideto Yamada","doi":"10.1002/rmb2.70049","DOIUrl":"https://doi.org/10.1002/rmb2.70049","url":null,"abstract":"Background: Anti-β2GPI/HLA-DR autoantibodies may be involved in recurrent implantation failure (RIF) and recurrent pregnancy loss (RPL). We examined their association with the endometrial microbiome and chronic endometritis (CE).Methods: In this cross-sectional study, 141 women (54 RIF, 87 RPL) were enrolled. Serum anti-β2GPI/HLA-DR positivity was defined using 99th/95th percentile cut-offs. Endometrial microbiome was assessed by 16S rRNA sequencing, focusing on reproductive-failure-related species (Gardnerella, Prevotella, Atopobium, Dialister, Anaerococcus, Ureaplasma, Mycoplasma). Microbiome and CE were compared by antibody status in RIF and RPL.Results: In RIF, antibody-positive women more frequently had Lactobacillus iners (99‰ and 95‰ cut-offs: 71.4% vs. 23.4%, p = 0.03; and 70.0% vs. 20.5%, p = 0.026) and reproductive-failure-related bacterial species (99‰: 100% vs. 51.1%, p = 0.016; 95‰: 90.0% vs. 52.3%, p = 0.032). In multivariable analysis with 95‰ cut-off, Lactobacillus iners (OR 13.1, p = 0.003) and reproductive-failure-related species (OR 9.64, p = 0.029) were independently associated with antibody positivity. In RPL, Anaerococcus was more frequent in antibody-positive women. CE frequency did not differ by antibody status in RIF or RPL.Conclusion: Anti-β2GPI/HLA-DR antibody positivity was associated with endometrial dysbiosis and may serve as a biomarker of abnormal intrauterine environment in reproductive failure.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70049"},"PeriodicalIF":3.3,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13066496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147676217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and Function of Meflin in Human Endometrial Decidualization. Meflin在人子宫内膜去个体化中的表达及功能。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-04-04 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70048

Naoki Inoue, Yumiko Tsukui, Akihito Morita, Yoshikazu Kitahara, Takashi Hirakawa, Tomoko Nakamura, Akira Iwase

{"title":"Expression and Function of Meflin in Human Endometrial Decidualization.","authors":"Naoki Inoue, Yumiko Tsukui, Akihito Morita, Yoshikazu Kitahara, Takashi Hirakawa, Tomoko Nakamura, Akira Iwase","doi":"10.1002/rmb2.70048","DOIUrl":"10.1002/rmb2.70048","url":null,"abstract":"Purpose: Decidualization of endometrial stromal cells involves significant morphological and functional changes in which alpha-smooth muscle actin (αSMA) expression decreases. Meflin, a glycosylphosphatidylinositol-anchored protein, suppresses αSMA expression and inhibits fibrosis in various tissues. Therefore, we investigated whether Meflin regulates decidualization by modulating αSMA expression.Methods: Meflin protein expression was evaluated in human endometrial tissues using immunohistochemistry. Immortalized endometrial stromal cells were used for in vitro experiments. The effects of decidualization on Meflin were assessed using 17β-estradiol, progesterone, and dibutyryl cyclic AMP treatments and analyzed using quantitative polymerase chain reaction, western blotting, and collagen gel contraction assays. The role of Meflin was investigated by performing an overexpression assay.Results: Meflin expression was significantly higher in the secretory-phase endometrium than in the proliferative-phase endometrium and was inversely correlated with αSMA expression. In vitro decidualization increased Meflin expression while suppressing that of αSMA. Meflin overexpression decreased αSMA expression and contractility, induced morphological changes from spindle-shaped to polygonal cells, and attenuated IGFBP1 expression, a decidualization marker.Conclusion: Meflin functions as a regulator of endometrial decidualization by modulating αSMA expression and cellular contractility. The evaluation of Meflin expression may provide insights into disorders associated with impaired decidualization, such as infertility and endometriosis.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70048"},"PeriodicalIF":3.3,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13051980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147634039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucocorticoids Improve in Vitro Mouse Oocyte Competence by Mimicking the Physiological Pre-Ovulatory Environment. 糖皮质激素通过模拟生理排卵前环境提高体外小鼠卵母细胞能力。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-04-02 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70046

Shahrina Akter, Takahiro Yamanaka, Asako Okamoto, Md Faizul Hossain Miraz, Masayuki Shimada

{"title":"Glucocorticoids Improve in Vitro Mouse Oocyte Competence by Mimicking the Physiological Pre-Ovulatory Environment.","authors":"Shahrina Akter, Takahiro Yamanaka, Asako Okamoto, Md Faizul Hossain Miraz, Masayuki Shimada","doi":"10.1002/rmb2.70046","DOIUrl":"https://doi.org/10.1002/rmb2.70046","url":null,"abstract":"Purpose: Ovulation is a highly regulated inflammatory process that involves the initiation and timely resolution of the inflammation for efficient oocyte maturation. During this process, glucocorticoids accumulate in the pre-ovulatory follicular environment as an anti-inflammatory mediator. However, their temporal regulation, endogenous synthesis, and functional significance on oocyte maturation and subsequent developmental competence remain unclear.Methods: Corticosterone levels in reproductive tract fluid and serum, alongside the expression and localization of related markers, including glucocorticoid receptors (NR3C1), were analyzed in granulosa cells (GC) and cumulus cells (CC) during gonadotropin stimulation. The functional relevance of corticosterone was evaluated by inhibiting endogenous corticosterone synthesis using metyrapone (MET) and by supplementing exogenously during in vitro maturation (IVM) conditions.Results: Human chorionic gonadotropin (hCG) increased follicular corticosterone at 8 h and upregulated steroidogenic markers (Cyp11a1, Cyp11b1, and Cyp21a1) while downregulating the inactivating enzyme Hsd11b2. MET significantly inhibited FSH-mediated endogenous corticosterone synthesis, resulting in poor IVM outcomes, and supplementation of exogenous corticosterone enhanced the IVM outcome, mitigated the MET effect, and maintained inflammatory balance.Conclusions: Gonadotropin induces a transient surge of ovarian corticosterone that supports in vivo maturation. Supplementing physiological levels of corticosterone during in vitro maturation (IVM) promotes oocyte maturation probably through cumulus cells.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70046"},"PeriodicalIF":3.3,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13045403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147623382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphokinetic Markers of Blastocyst Formation and Chromosomal Euploidy in Embryos Derived From In Vitro Maturation Oocytes. 体外成熟卵母细胞胚胎胚泡形成和染色体整倍体的形态动力学标记。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2026-03-30 eCollection Date: 2026-01-01 DOI: 10.1002/rmb2.70037

Hong Chen, Ruolin Mao, Rui Long, Juepu Zhou, Huijun Li, Qingsong Xi, Lixia Zhu, Lei Jin

{"title":"Morphokinetic Markers of Blastocyst Formation and Chromosomal Euploidy in Embryos Derived From In Vitro Maturation Oocytes.","authors":"Hong Chen, Ruolin Mao, Rui Long, Juepu Zhou, Huijun Li, Qingsong Xi, Lixia Zhu, Lei Jin","doi":"10.1002/rmb2.70037","DOIUrl":"https://doi.org/10.1002/rmb2.70037","url":null,"abstract":"Purpose: To characterize the morphokinetic parameters of embryos derived from IVM oocytes and to identify critical factors associated with blastocyst formation potential and chromosomal euploidy.Methods: This retrospective study reviewed 99 523 fresh cycles between January 2019 and March 2025. From this population, 3,202 PGT cycles were initially screened. A total of 353 cycles involving IVM oocytes were then selected, and ultimately, 83 IVM-derived embryos from 59 cycles were included.Results: The non-formation group exhibited delays in t2, t9, and several developmental intervals, including S2, t9-t7, t9-t8 (p < 0.05). Multivariate logistic regression analysis identified S2 (p = 0.021) and t9-t7 (p = 0.042) as independent factors associated with blastocyst formation. Subsequently, ROC analysis showed that both S2 ≤ 1.95 h (AUC = 0.620, p = 0.036) and t9-t7 ≤ 24.35 h (AUC = 0.672, p < 0.001) exhibited predictive power for blastocyst formation. Furthermore, shorter tSB-t9 and tB-t9 were associated with euploidy in IVM-derived blastocysts (p = 0.025 and p = 0.014, respectively), with tSB-t9 emerging as an independent factor associated with chromosomal normality (p = 0.044) in multivariate analysis.Conclusions: This study presents the morphokinetic characteristics and identifies key markers for blastocyst formation and chromosomal euploidy in IVM-derived embryos.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"25 1","pages":"e70037"},"PeriodicalIF":3.3,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13045424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147623558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0