Reproductive Medicine and Biology最新文献

Feasibility of SNP Genotyping Using Dried Blood Spot Samples Collected in an Epidemiological Study and Its Integration With Genetic Risk Analysis for Endometriosis. 流行病学研究中采集的干血斑样本SNP基因分型的可行性及其与子宫内膜异位症遗传风险分析的整合。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2025-09-10 eCollection Date: 2025-01-01 DOI: 10.1002/rmb2.12675

Yoshikazu Kitahara, Yuki Ideno, Kensaku Tomiyoshi, Yoko Onizuka, Kazue Nagai, Akira Iwase, Junko Shimada, Hiroshi Ohnishi, Kunihiko Hayashi

{"title":"Feasibility of SNP Genotyping Using Dried Blood Spot Samples Collected in an Epidemiological Study and Its Integration With Genetic Risk Analysis for Endometriosis.","authors":"Yoshikazu Kitahara, Yuki Ideno, Kensaku Tomiyoshi, Yoko Onizuka, Kazue Nagai, Akira Iwase, Junko Shimada, Hiroshi Ohnishi, Kunihiko Hayashi","doi":"10.1002/rmb2.12675","DOIUrl":"10.1002/rmb2.12675","url":null,"abstract":"Purpose: This study evaluated the feasibility of single-nucleotide polymorphism (SNP) genotyping using dried blood spot (DBS) samples stored under various conditions, based on the genotyping success rate and concordance with whole blood results. It also examined associations between selected SNPs and endometriosis risk in Japanese women.Methods: DBS samples from 41 cohort participants and 28 hospital patients were used to assess genotyping feasibility. Five endometriosis-associated SNPs-rs10965235, rs12700667, rs12024204, rs16826658, and rs801112-were genotyped in 37 cases and 144 controls. Genotype distributions were evaluated for Hardy-Weinberg equilibrium (HWE) using Pearson's χ 2 test or, when appropriate, Fisher's exact test, with a significance threshold of p < 0.05. Fisher's exact test was used for association analysis.Results: SNP genotyping for rs12700667 showed 100% success and complete concordance between DBS and whole blood samples under all storage conditions. Four of five SNPs met HWE, while rs10965235 significantly deviated from it (p = 0.0225). The CC genotype of rs10965235 was potentially associated with lower endometriosis risk (odds ratio: 0.19), although this was not statistically significant after correction.Conclusions: DBS is a robust DNA source for SNP genotyping under various conditions and suitable for mail-based epidemiological studies. Population-specific validation is essential when applying GWAS findings.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12675"},"PeriodicalIF":3.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "Preimplantation Genetic Testing for Aneuploidy and Chromosomal Structural Rearrangement: A Summary of a Nationwide Study by the Japan Society of Obstetrics and Gynecology". 更正“胚胎植入前基因检测非整倍体和染色体结构重排：日本妇产科学会的一项全国性研究总结”。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2025-09-08 eCollection Date: 2025-01-01 DOI: 10.1002/rmb2.12676

引用次数: 0

Sperm Injection at the Para-Polar Body Site in Piezo-Intracytoplasmic Sperm Injection Improves Subsequent Early Development of Bovine Embryos. 压电胞浆内单精子注射中对极体部位的精子注射改善了随后的牛胚胎早期发育。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2025-09-01 eCollection Date: 2025-01-01 DOI: 10.1002/rmb2.12660

Shiori Ashibe, Yoku Kato, Sarentonglaga Borjigin, Yoshikazu Nagao

{"title":"Sperm Injection at the Para-Polar Body Site in Piezo-Intracytoplasmic Sperm Injection Improves Subsequent Early Development of Bovine Embryos.","authors":"Shiori Ashibe, Yoku Kato, Sarentonglaga Borjigin, Yoshikazu Nagao","doi":"10.1002/rmb2.12660","DOIUrl":"10.1002/rmb2.12660","url":null,"abstract":"Purpose: In piezo-ICSI, the first polar body (PB) of a metaphase II (MII) oocyte is generally oriented in the 6 or 12 o'clock position relative to sperm injection at 3 o'clock. However, the ooplasmic cell membrane may be damaged during drilling of the zona pellucida by piezo pulses. Here, we tested a new piezo-ICSI method in which the PB is set at the 2 or 4 o'clock position, so that zona drilling is performed through the widest position in the perivitelline space (para-PB piezo).Methods: The effect of piezo pulse position was evaluated by propidium iodide staining and cytoplasmic Ca2+ levels. The effect of injection site on integrity and movement of the meiotic spindle was evaluated by microscopy and an Oosight imaging system. Bovine oocyte survival rate, developmental competence, and chromosomal integrity at the blastocyst stage were compared between para-PB piezo and conventional piezo groups.Results: Piezo pulses may induce slight changes in the ooplasmic cell; our piezo-ICSI method can improve oocyte survival by minimizing damage to the ooplasmic cell membrane during zona drilling and consequently increasing the number of embryos available for transfer.Conclusions: Para-PB site piezo-ICSI is an improvement on current ICSI technology for animal and human reproduction.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12660"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Nucleated Red Blood Cells With Intact Genomic DNA From Cord Blood by Applying G&T-Seq. 应用gt - seq技术从脐带血中分离具有完整基因组DNA的有核红细胞。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2025-09-01 eCollection Date: 2025-01-01 DOI: 10.1002/rmb2.12671

Noriko Ito, Tatsuya Fujii, Kosuke Taniguchi, Yuka Okazaki, Hiroko Ogata-Kawata, Haruhiko Sago, Kenichiro Hata, Kazuhiko Nakabayashi

{"title":"Isolation of Nucleated Red Blood Cells With Intact Genomic DNA From Cord Blood by Applying G&T-Seq.","authors":"Noriko Ito, Tatsuya Fujii, Kosuke Taniguchi, Yuka Okazaki, Hiroko Ogata-Kawata, Haruhiko Sago, Kenichiro Hata, Kazuhiko Nakabayashi","doi":"10.1002/rmb2.12671","DOIUrl":"10.1002/rmb2.12671","url":null,"abstract":"Purpose: Fetal cells in maternal blood are a pure source of fetal genomic DNA for noninvasive prenatal testing (NIPT), if successfully isolated. We assessed whether single-cell genome and transcriptome sequencing (G&T-seq) can be applied to efficiently isolate fetal nucleated red blood cells (fNRBCs) suitable for genetic testing.Methods: Using umbilical cord blood as a model, we isolated 165 single NRBC candidates from four samples and 12 single lymphocytes as controls from one sample. G&T-seq was used to estimate the maturation stage of each NRBC candidate from the transcriptome data, and genomic integrity was assessed using shallow whole-genome sequencing (WGS) data.Results: Multi-dimensional scaling (MDS) of the transcriptome data revealed that five NRBC candidates clustered separately, classifying them as primitive NRBCs. Two of these cells showed high yields of WGS libraries and high mapping rates comparable to control lymphocytes, suggesting an intact nuclear genome.Conclusions: G&T-seq effectively identified primitive NRBCs with high-quality DNA among candidate cells dominated by mature RBCs. Single-cell multi-omics technology may advance the development of fNRBC-based NIPT.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12671"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comment on "Clinical Significance of Biochemical Pregnancy Loss in Recurrent Pregnancy Loss Patients: Insights From Euploid Embryo Transfers Minimizing Embryonic Bias". 评论“复发性妊娠丢失患者生化妊娠丢失的临床意义：来自整倍体胚胎移植的见解，最大限度地减少胚胎偏差”。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2025-09-01 eCollection Date: 2025-01-01 DOI: 10.1002/rmb2.12674

Prajnasini Satapathy, Rachana Mehta, Ranjana Sah

引用次数: 0

Significance of Mitochondrial Dynamics in Reproductive Physiology: Current and Emerging Horizons in Mitochondrial Therapy for Assisted Reproductive Technologies. 线粒体动力学在生殖生理学中的意义：辅助生殖技术中线粒体治疗的现状和新视野。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2025-08-17 eCollection Date: 2025-01-01 DOI: 10.1002/rmb2.12672

Sanath Udayanga Kankanam Gamage, Yoshiharu Morimoto

{"title":"Significance of Mitochondrial Dynamics in Reproductive Physiology: Current and Emerging Horizons in Mitochondrial Therapy for Assisted Reproductive Technologies.","authors":"Sanath Udayanga Kankanam Gamage, Yoshiharu Morimoto","doi":"10.1002/rmb2.12672","DOIUrl":"10.1002/rmb2.12672","url":null,"abstract":"Background: Mitochondria play a critical role in cellular bioenergetics and signaling, with particular importance in the context of reproductive biology. This review summarizes their role in reproduction and explores current and emerging mitochondrial therapies for fertility treatment.Methods: A comprehensive literature search using terms like mitochondria, infertility, reproduction, gametes, mitochondrial replacement, and mitochondrial transplantation identified relevant studies on mitochondria's role in gametogenesis, fertilization, and early embryonic development in relevant databases. Selected publications were reviewed and summarized to present current and future mitochondrial therapies for fertility.Main findings: Mitochondrial dynamics and functions are critical for meeting the energy requirements of essential reproductive processes, including gametogenesis, fertilization, and early embryonic development. Dysregulation of mitochondrial function has been associated with a range of reproductive disorders, such as infertility, recurrent pregnancy loss, and maternally inherited mitochondrial diseases. Emerging therapeutic strategies, such as mitochondrial replacement therapy, antioxidant supplementation, and mitochondrial transplantation, offer promising avenues for overcoming these challenges and improving reproductive outcomes.Conclusions: Utilizing mitochondrial-based therapies represents a promising and innovative approach in the advancement of fertility treatments. Ongoing research and clinical development in this area hold significant potential to enhance reproductive outcomes and improve the quality of life for individuals and couples facing fertility challenges.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12672"},"PeriodicalIF":3.3,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12358321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive Outcomes of Transferring Blastocysts Derived From Frozen-Thawed Cleavage Embryos: A Systematic Review and Meta-Analysis. 冷冻解冻卵裂胚胎移植囊胚的生殖结果：系统综述和荟萃分析。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2025-08-07 eCollection Date: 2025-01-01 DOI: 10.1002/rmb2.12673

Huy Phuong Tran, Nam Nhat Nguyen, Ngoc Thanh Truong, Tuyet Thi-Diem Hoang, Trang Nguyen-Khanh Huynh, Ha Le-Bao Tran

{"title":"Reproductive Outcomes of Transferring Blastocysts Derived From Frozen-Thawed Cleavage Embryos: A Systematic Review and Meta-Analysis.","authors":"Huy Phuong Tran, Nam Nhat Nguyen, Ngoc Thanh Truong, Tuyet Thi-Diem Hoang, Trang Nguyen-Khanh Huynh, Ha Le-Bao Tran","doi":"10.1002/rmb2.12673","DOIUrl":"10.1002/rmb2.12673","url":null,"abstract":"Background: In in vitro fertilization (IVF), transferring frozen-thawed blastocysts is a widely adopted practice. This meta-analysis aims to evaluate the reproductive outcomes of transferring blastocysts derived from frozen-thawed cleavage embryos (FT-CDB group) compared to direct frozen-thawed blastocyst (DFB group) transfers.Methods: We searched the following electronic databases for relevant studies: PubMed/MEDLINE, EMBASE, Scopus, and Web of Science. Studies were included if they compared the clinical and neonatal outcomes of IVF patients receiving either FT-CDB or DFB transfer with vitrification method. The protocol for this review has been registered in PROSPERO.Results: A total of seven studies (2057 patients) were included in the analysis. Participants in the FT-CDB group demonstrated significantly higher odds of achieving clinical pregnancy (OR 1.24, 95% CI 1.03-1.49, p = 0.022, I 2 = 27%), and live birth (OR 1.31, 95% CI 1.08-1.60, p = 0.007, I 2 = 0%) compared to the DFB group. No significant differences were observed in the birth weights of infants between the groups (MD -87.05 g, 95% CI -293.77 to 119.67, p = 0.41, I 2 = 83%).Conclusion: Transferring blastocysts derived from frozen-thawed cleavage embryos is associated with higher odds of clinical pregnancy and live birth compared to frozen-thawed blastocyst transfers.Trial registration: PROSPERO number: CRD42024591620.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12673"},"PeriodicalIF":3.3,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144800118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Systematic Evidence-Based Review Regarding miRNA Polymorphisms in Recurrent Implantation Failure. 复发性植入失败中miRNA多态性的系统性循证综述。

IF 3.3 3区医学

Reproductive Medicine and Biology Pub Date : 2025-07-30 eCollection Date: 2025-01-01 DOI: 10.1002/rmb2.12670

Bogdan Doroftei, Ovidiu-Dumitru Ilie, Ana-Maria Dabuleanu, Mara Doroftei, Ciprian Ilea, Sergiu Timofeiov, Anca Bivoleanu, Elena Tataranu

{"title":"A Systematic Evidence-Based Review Regarding miRNA Polymorphisms in Recurrent Implantation Failure.","authors":"Bogdan Doroftei, Ovidiu-Dumitru Ilie, Ana-Maria Dabuleanu, Mara Doroftei, Ciprian Ilea, Sergiu Timofeiov, Anca Bivoleanu, Elena Tataranu","doi":"10.1002/rmb2.12670","DOIUrl":"10.1002/rmb2.12670","url":null,"abstract":"Background: This systematic review aimed to evaluate whether specific single nucleotide polymorphisms (SNPs) in miRNAs are associated with recurrent implantation failure (RIF).Methods: A comprehensive literature search was conducted across PubMed-MEDLINE, Web of Science, Scopus, and the Excerpta Medica DataBASE.Results: The Newcastle-Ottawa Scale (NOS) yielded an intermediate to high quality, with one study rated with 6 stars, and the remaining four with 7 stars. RIF risk-related genotypes included miR-196a, miR-449b, miR-34a, miR-146aCG+GG-miR-196a2CC, miR-149TT-miR-196a2CC, miR-196a2CC-miR-499AA, miR-608GC-miR-938CC, miR-27aAG-miR-423CC/miR-604AG/GG and miR-34aC>A AA-miR-130aG>A GG. Protective combinations included miR-1302-3, miR-631II-miR-1302-3CT, and miR-938CC-miR-1302-3CT. Protective allele combinations G-T-T-A, C-T, T-T-G, T-T and G-C-A-G, G-A-G, A-G-G were less frequent in RIF cases, whereas A-T-C, T-C-C-T, T-C-T, A-C-G-A, A-A-G-G, G-A-A-A, A-A-C-A and G-G-A haplotypes were more commonly associated with increased risk. Notably, miR-608 GC+CC, miR-1302-3 CC, miR-27a AG+GG, miR-423 CA+AA, miR-604 AG+GG, miR-222 GT+TT, and miR-34a GA+AA were associated with altered coagulation parameters. Additionally, miR-222 correlated with decreased creatinine levels, the G>T mutation with elevated follicle-stimulating hormone (FSH), miR-34aC>A AA genotype with reduced thyroid-stimulating hormone (TSH) levels, and CA+AA with increased blood urea nitrogen (BUN) levels.Conclusions: This systematic review highlights that specific miRNA SNPs and haplotype combinations are significantly associated with either increased susceptibility to or protection against RIF.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12670"},"PeriodicalIF":3.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12309981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144754087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Significance of Biochemical Pregnancy Loss in Recurrent Pregnancy Loss Patients: Insights From Euploid Embryo Transfers Minimizing Embryonic Bias. 生化妊娠丢失在复发性妊娠丢失患者中的临床意义：来自整倍体胚胎移植最小化胚胎偏差的见解。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2025-07-23 eCollection Date: 2025-01-01 DOI: 10.1002/rmb2.12668

Yoshimitsu Kuwabara, Tatsunori Shiraishi, Ryoko Kato, Shigeru Matsuda, Akiko Sakata, Yumene Kubota, Ryoko Yokote, Kimihiko Nakao, Mirei Yonezawa, Tomoko Ichikawa, Toshiyuki Takeshita, Shunji Suzuki

{"title":"Clinical Significance of Biochemical Pregnancy Loss in Recurrent Pregnancy Loss Patients: Insights From Euploid Embryo Transfers Minimizing Embryonic Bias.","authors":"Yoshimitsu Kuwabara, Tatsunori Shiraishi, Ryoko Kato, Shigeru Matsuda, Akiko Sakata, Yumene Kubota, Ryoko Yokote, Kimihiko Nakao, Mirei Yonezawa, Tomoko Ichikawa, Toshiyuki Takeshita, Shunji Suzuki","doi":"10.1002/rmb2.12668","DOIUrl":"10.1002/rmb2.12668","url":null,"abstract":"Purpose: To evaluate the clinical relevance of biochemical pregnancy loss (BPL) in recurrent pregnancy loss (RPL) patients, using data from preimplantation genetic testing for aneuploidy (PGT-A) to minimize embryonic factors.Methods: This retrospective cohort study included 52 PGT-A cycles (48 patients) with single euploid embryo transfers between April 2020 and December 2022. Patients were stratified into three groups: Group A (ART failure without RPL, 18 cycles/17 patients), Group B (RPL following ART pregnancies, 12 cycles/10 patients), and Group C (RPL following natural pregnancies, 22 cycles/21 patients). This classification aimed to assess maternal factors contributing to BPL across different clinical backgrounds. The incidence of BPL, clinical pregnancy rate, and predictive performance of ART outcomes were analyzed, with and without BPL included, using ROC curve analysis.Results: Biochemical pregnancy loss occurred in 0% (A), 25.0% (B), and 37.5% (C) of patients (p = 0.037). Incorporating BPL into miscarriage history significantly improved ART outcome prediction (AUC 0.871 vs. 0.759).Conclusion: Biochemical pregnancy loss after euploid embryo transfer likely reflects maternal or endometrial pathology. Incorporating BPL into the diagnostic criteria for RPL may enhance clinical assessment and personalized care.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12668"},"PeriodicalIF":2.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12284377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Status of In Vitro Oocyte Growth and Development in Mammals. 哺乳动物体外卵母细胞生长发育的现状。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2025-07-17 eCollection Date: 2025-01-01 DOI: 10.1002/rmb2.12669

Yuji Hirao

{"title":"Current Status of In Vitro Oocyte Growth and Development in Mammals.","authors":"Yuji Hirao","doi":"10.1002/rmb2.12669","DOIUrl":"10.1002/rmb2.12669","url":null,"abstract":"Background: In vitro-matured oocytes play an increasingly vital role in livestock production and fertility treatments. However, oocytes grown in vitro are not yet practical for widespread use. So far, only mouse oocytes have achieved full developmental competence granted in vitro from the early growth stage.Methods: This review provides an overview of established culture methods and conditions, analyzing their effects. When evaluating studies, outcomes specifically related to in vitro-grown (IVG) oocytes rather than the follicle were prioritized.Main findings: Neonatal mouse first-wave oocytes show a hypothesized linear volume increase, and IVG mouse oocytes have followed a similar developmental timeline across multiple studies. In other species, a proportion of bovine oocytes from early antral follicles achieved full size during 2-week cultures, with some producing viable offspring, confirming developmental competence. Preantral follicle culture systems are typically designed to address specific research parameters, such as developmental competence assessment. Preimplantation embryogenesis has been reported in several species, including humans.Conclusion: For species with oocytes ≥ 125 μm in diameter, normal growth can be achieved during the final 2 weeks of growth as shown in bovines. However, developing culture systems that can maintain follicle viability for the preceding 4-5 weeks remains a critical challenge.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"24 1","pages":"e12669"},"PeriodicalIF":2.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0