Reproductive Medicine and Biology最新文献_第8页

Identification of long noncoding RNAs downregulated specifically in ovarian high-grade serous carcinoma. 鉴定卵巢高级别浆液性癌中特异性下调的长非编码 RNA。

IF 4.6 3区医学

Reproductive Medicine and Biology Pub Date : 2024-04-03 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12572

Maki Hayashi-Okada, Shun Sato, Kengo Nakashima, Takahiro Sakai, Tetsuro Tamehisa, Takuya Kajimura, Isao Tamura, Kotaro Sueoka, Norihiro Sugino

{"title":"Identification of long noncoding RNAs downregulated specifically in ovarian high-grade serous carcinoma.","authors":"Maki Hayashi-Okada, Shun Sato, Kengo Nakashima, Takahiro Sakai, Tetsuro Tamehisa, Takuya Kajimura, Isao Tamura, Kotaro Sueoka, Norihiro Sugino","doi":"10.1002/rmb2.12572","DOIUrl":"10.1002/rmb2.12572","url":null,"abstract":"Purpose: To investigate whether long noncoding RNAs (lncRNAs) are involved in the development or malignant behavior of ovarian high-grade serous carcinoma (HGSC), we attempted to identify lncRNAs specific to HGSC.Methods: Total RNAs were isolated from HGSC, normal ovarian, and fallopian tube tissue samples and were subjected to a PCR array that can analyze 84 cancer-associated lncRNAs. The lncRNAs that were upregulated and downregulated in HGSC in comparison to multiple samples of normal ovary and fallopian tube were validated by real-time RT-PCR. To infer the function, ovarian cancer cell lines that overexpress the identified lncRNAs were established, and the activation of cell proliferation, migration, and invasion was analyzed.Results: Eleven lncRNAs (ACTA2-AS1, ADAMTS9-AS2, CBR3-AS1, HAND2-AS1, IPW, LINC00312, LINC00887, MEG3, NBR2, TSIX, and XIST) were downregulated in HGSC samples. We established the cell lines that overexpress ADAMTS9-AS2, CBR3-AS1, or NBR2. In cell lines overexpressing ADAMTS9-AS2, cell proliferation was suppressed, but migration and invasion were promoted. In cell lines overexpressing CBR3-AS1 or NBR2, cell migration tended to be promoted, although cell proliferation and invasion were unchanged.Conclusion: We identified eleven lncRNAs that were specifically downregulated in HGSC. Of these, CBR3-AS1, NBR2, and ADAMTS9-AS2 had unique functions in the malignant behaviors of HGSC.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12572"},"PeriodicalIF":4.6,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10988898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular crosstalk between insulin-like growth factors and follicle-stimulating hormone in the regulation of granulosa cell function. 胰岛素样生长因子和卵泡刺激素在调控颗粒细胞功能中的分子串扰。

IF 4.6 3区医学

Reproductive Medicine and Biology Pub Date : 2024-04-03 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12575

Emily Hayes, Nicola Winston, Carlos Stocco

{"title":"Molecular crosstalk between insulin-like growth factors and follicle-stimulating hormone in the regulation of granulosa cell function.","authors":"Emily Hayes, Nicola Winston, Carlos Stocco","doi":"10.1002/rmb2.12575","DOIUrl":"10.1002/rmb2.12575","url":null,"abstract":"Background: The last phase of folliculogenesis is driven by follicle-stimulating hormone (FSH) and locally produced insulin-like growth factors (IGFs), both essential for forming preovulatory follicles.Methods: This review discusses the molecular crosstalk of the FSH and IGF signaling pathways in regulating follicular granulosa cells (GCs) during the antral-to-preovulatory phase.Main findings: IGFs were considered co-gonadotropins since they amplify FSH actions in GCs. However, this view is not compatible with data showing that FSH requires IGFs to stimulate GCs, that FSH renders GCs sensitive to IGFs, and that FSH signaling interacts with factors downstream of AKT to stimulate GCs. New evidence suggests that FSH and IGF signaling pathways intersect at several levels to regulate gene expression and GC function.Conclusion: FSH and locally produced IGFs form a positive feedback loop essential for preovulatory follicle formation in all species. Understanding the mechanisms by which FSH and IGFs interact to control GC function will help design new interventions to optimize follicle maturation, perfect treatment of ovulatory defects, improve in vitro fertilization, and develop new contraceptive approaches.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12575"},"PeriodicalIF":4.6,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10988955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel erectile analyses revealed augmentable penile Lyve-1, the lymphatic marker, expression. 新的勃起分析表明，阴茎淋巴标志物 Lyve-1 的表达增强。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-04-01 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12570

Kota Fujimoto, Daiki Hashimoto, Sang Woon Kim, Yong Seung Lee, Takuya Suzuki, Masanori Nakata, Shinji Kumegawa, Shinichi Asamura, Gen Yamada

{"title":"Novel erectile analyses revealed augmentable penile Lyve-1, the lymphatic marker, expression.","authors":"Kota Fujimoto, Daiki Hashimoto, Sang Woon Kim, Yong Seung Lee, Takuya Suzuki, Masanori Nakata, Shinji Kumegawa, Shinichi Asamura, Gen Yamada","doi":"10.1002/rmb2.12570","DOIUrl":"10.1002/rmb2.12570","url":null,"abstract":"Purpose: The pathophysiology of penis extends to erectile dysfunction (ED) to conditions including sexually transmitted diseases (STDs) and cancer. To date, there has been little research evaluating vascular drainage from the penis. We aimed to evaluate penile blood flow in vivo and analyze its possible relationship with the lymphatic maker.Materials and methods: We established an in vivo system designed to assess the dynamic blood outflow from the corpus cavernosum (CC) by dye injection. To analyze lymphatic characteristics in the CC, the expression of Lyve-1, the key lymphatic endothelium marker, was examined by the in vitro system and lipopolysaccharide (LPS) injection to mimic the inflammatory conditions.Results: A novel cavernography methods enable high-resolution morphological and functional blood drainage analysis. The expression of Lyve-1 was detected along the sinusoids. Furthermore, its prominent expression was also observed after penile LPS injection and in the erectile condition.Conclusions: The current in vivo system will potentially contribute to the assessment of penile pathology from a novel viewpoint. In addition, current analyses revealed inducible Lyve-1 expression for LPS injection and the erection state, which requires further analyses on penile lymphatic system.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12570"},"PeriodicalIF":2.7,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10985380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of paternal ionizing radiation exposure on fertility and offspring's health. 父亲电离辐射照射对生育和后代健康的影响。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-03-25 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12567

Jiaqun Li, Juan Liu, Yanye Zhang, Hong Qiu, Junyan Zheng, Jinglei Xue, Jiani Jin, Feida Ni, Chunxi Zhang, Chuan Chen, Xiao Sun, Huiquan Wang, Dan Zhang

{"title":"Effects of paternal ionizing radiation exposure on fertility and offspring's health.","authors":"Jiaqun Li, Juan Liu, Yanye Zhang, Hong Qiu, Junyan Zheng, Jinglei Xue, Jiani Jin, Feida Ni, Chunxi Zhang, Chuan Chen, Xiao Sun, Huiquan Wang, Dan Zhang","doi":"10.1002/rmb2.12567","DOIUrl":"10.1002/rmb2.12567","url":null,"abstract":"Purpose: The intergenerational effects of ionizing radiation remain controversial. Extensive insights have been revealed for DNA mutations and cancer incidence in progeny, yet many of these results were obtained by immediate post-radiation mating. However, conception at short times after radiation exposure is likely to be avoided. After a long period of fertility recovery, whether unexposed sperm derived from exposed spermatogonia would challenge the health of the offspring is not yet clearly demonstrated.Methods: Ten-week-old C57BL/6J males underwent whole-body acute γ irradiation at 0 and 6.4 Gy. Testes and sperm were collected at different times after radiation to examine reproductive changes. The reproductive, metabolic, and neurodevelopmental parameters were measured in the offspring of controls and the offspring derived from irradiated undifferentiated spermatogonia.Results: Paternal fertility was lost after acute 6.4 Gy γ radiation and recovered at 10-11 weeks post irradiation in mice. The reproductive, metabolic, and neurodevelopmental health of offspring born to irradiated undifferentiated spermatogonia were comparable to those of controls.Conclusion: The male mice could have healthy offspring after recovery from the damage caused by ionizing radiation.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12567"},"PeriodicalIF":2.7,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10961711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140288861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy and safety of follitropin delta versus follitropin alpha/beta in infertility treatment: A systematic review and meta-analysis. 在不孕症治疗中，follitropin delta 与 follitropin alpha/beta 的疗效和安全性对比：系统回顾与荟萃分析。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-03-25 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12573

Shinnosuke Komiya, Jun Watanabe, Takero Terayama, Kyosuke Kamijo, Hidetaka Okada

{"title":"Efficacy and safety of follitropin delta versus follitropin alpha/beta in infertility treatment: A systematic review and meta-analysis.","authors":"Shinnosuke Komiya, Jun Watanabe, Takero Terayama, Kyosuke Kamijo, Hidetaka Okada","doi":"10.1002/rmb2.12573","DOIUrl":"10.1002/rmb2.12573","url":null,"abstract":"Background: Follitropin δ may be an alternative to conventional follitropin α/β for controlled ovarian stimulation (COS) within assisted reproductive treatment (ART), but its efficacy and safety remain unknown. We performed a random-effects meta-analysis to compare the efficacy and safety of follitropin δ and follitropin α/β.Methods: We searched randomized controlled trials comparing follitropin δ and follitropin α/β using MEDLINE, Embase, CENTRAL, ClinicalTrials.gov, and WHO-ITCRP on December 14, 2022. The primary outcomes were the live birth rate and the incidence of moderate or severe ovarian hyperstimulation syndrome (OHSS). The certainty of the evidence was assessed using the grading of recommendations assessment, development, and evaluation approach. The protocol was registered on the Open Science Framework.Results: Three studies involving 2682 participants were included in our meta-analysis. The results indicated that follitropin δ may result in little to no difference in live birth rates (risk ratio [RR], 1.12; 95% confidence interval [CI], 0.91-1.38; low certainty) and the incidence of moderate or severe OHSS (RR, 0.78; 95% CI, 0.48-1.26; low certainty) compared with follitropin α/β.Conclusion: Follitropin δ may result in little to no difference in COS compared with follitropin α/β, especially in terms of live births and safety.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12573"},"PeriodicalIF":2.7,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10961712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140288862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurotensin induces sustainable activation of the ErbB-ERK1/2 pathway, which is required for developmental competence of oocytes in mice. 神经营养素诱导 ErbB-ERK1/2 通路的持续激活，这是小鼠卵母细胞发育能力所必需的。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-03-20 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12571

Asako Okamoto, Tomoya Nakanishi, Shingo Tonai, Masayuki Shimada, Yasuhisa Yamashita

{"title":"Neurotensin induces sustainable activation of the ErbB-ERK1/2 pathway, which is required for developmental competence of oocytes in mice.","authors":"Asako Okamoto, Tomoya Nakanishi, Shingo Tonai, Masayuki Shimada, Yasuhisa Yamashita","doi":"10.1002/rmb2.12571","DOIUrl":"10.1002/rmb2.12571","url":null,"abstract":"Purpose: LH induces the expression of EGF-like factors and their shedding enzyme (ADAM17) in granulosa cells (GCs), which is essential for ovulation via activation of the ErbB-ERK1/2 pathway in cumulus cells (CCs). Neurotensin (NTS) is reported as a novel regulator of ovulation, whereas the NTS-induced maturation mechanism in oocytes remains unclear. In this study, we focused on the role of NTS in the expression of EGF-like factors and ErbBs, and ADAM17 activity, during oocyte maturation and ovulation in mice.Methods: The expression and localization in GC and CC were examined. Next, hCG and NTS receptor 1 antagonist (SR) were injected into eCG-primed mice, and the effects of SR on ERK1/2 phosphorylation were investigated. Finally, we explored the effects of SR on the expression of EGF-like factors and ErbBs, and ADAM17 activity in GC and CC.Results: NTS was significantly upregulated in GC and CC following hCG injection. SR injection suppressed oocyte maturation and ERK1/2 phosphorylation. SR also downregulated part of the expression of EGF-like factors and their receptors, and ADAM17 activity.Conclusions: NTS induces oocyte maturation through the sustainable activation of the ERK1/2 signaling pathway by upregulating part of the EGF-like factor-induced pathway during oocyte maturation in mice.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12571"},"PeriodicalIF":2.7,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10951886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140176223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The roles of sex hormones in the pathophysiology of age-related sarcopenia and frailty. 性激素在与年龄相关的肌肉疏松症和虚弱的病理生理学中的作用。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-03-11 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12569

Tatsuya Hosoi, Mitsutaka Yakabe, Seiji Hashimoto, Masahiro Akishita, Sumito Ogawa

{"title":"The roles of sex hormones in the pathophysiology of age-related sarcopenia and frailty.","authors":"Tatsuya Hosoi, Mitsutaka Yakabe, Seiji Hashimoto, Masahiro Akishita, Sumito Ogawa","doi":"10.1002/rmb2.12569","DOIUrl":"10.1002/rmb2.12569","url":null,"abstract":"Background: Sarcopenia is an age-related condition characterized by a progressive and systemic decline in skeletal muscle mass, quality, and strength. The incidence of sarcopenia contains sex-specific aspects, indicating the contribution of sex hormones to its pathophysiology. This review focuses on changing trends in sarcopenia, discusses alterations in definitions and diagnostic criteria, and emphasizes the association between sarcopenia and sex hormones.Methods: A literature search was performed on PubMed for related articles published between 1997 and December 2023 using appropriate keywords.Main findings results: Advances in research have emphasized the significance of muscle quality and strength over muscle mass, resulting in new diagnostic criteria for sarcopenia. Androgens demonstrated anabolic effects on skeletal muscles and played a significant role in the pathophysiology of sarcopenia. In clinical settings, androgen replacement therapy has exhibited certain positive outcomes for treating sarcopenia, despite concerns about potential side effects. Conversely, estrogen is involved in skeletal muscle maintenance, but the detailed mechanisms remain unclear. Moreover, results regarding the clinical application of estrogen replacement therapy for treating sarcopenia remained inconsistent.Conclusion: The elucidation of molecular mechanisms that involve sex hormones is eagerly awaited for novel therapeutic interventions for sarcopenia.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12569"},"PeriodicalIF":2.7,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micromapping testicular sperm extraction: A new technique for microscopic testicular sperm extraction in nonobstructive azoospermia. 显微睾丸取精术：非梗阻性无精子症的显微睾丸取精新技术。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-03-11 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12566

Toshihiro Tai, Wakaba Miyamoto, Yuriko Fukuoka, Sena Shibasaki, Mizuho Takahashi, Noriyuki Okuyama, Hiromitsu Hattori, Ikue Ishikawa, Satoko Nagaura, Koki Yoshinaga, Masae Koizumi, Tomoko Hashimoto, Mayumi Toya, Jin Kumagai, Hideki Igarashi, Koichi Kyono

{"title":"Micromapping testicular sperm extraction: A new technique for microscopic testicular sperm extraction in nonobstructive azoospermia.","authors":"Toshihiro Tai, Wakaba Miyamoto, Yuriko Fukuoka, Sena Shibasaki, Mizuho Takahashi, Noriyuki Okuyama, Hiromitsu Hattori, Ikue Ishikawa, Satoko Nagaura, Koki Yoshinaga, Masae Koizumi, Tomoko Hashimoto, Mayumi Toya, Jin Kumagai, Hideki Igarashi, Koichi Kyono","doi":"10.1002/rmb2.12566","DOIUrl":"10.1002/rmb2.12566","url":null,"abstract":"Purpose: In microscopic testicular sperm extraction (mTESE) for nonobstructive azoospermia (NOA), sperm can be recovered relatively easily in some cases, and mTESE may be retrospectively considered excessive. However, mTESE is routinely performed in the majority of NOA patients because of the difficulty in predicting tissue status. A minimally invasive and comprehensive sperm retrieval method that allows on-the-spot tissue assessment is needed. We have developed and evaluated a novel sperm retrieval technique for NOA called micromapping testicular sperm extraction (MMTSE).Methods: MMTSE involves dividing the testis into four sections and making multiple small needle holes in the tunica albuginea to extract seminiferous tubules and retrieve sperm. The sperm-positive group by MMTSE (Group I) underwent additional tissue collection (ATC) via a small incision, whereas the sperm-negative group by MMTSE (Group 0) underwent mTESE.Results: In total, 40 NOA participants underwent MMTSE. Group I included 15 patients and Group 0 included 25 patients. In Group 1, sperm were recovered from all patients by ATC. In Group 0, sperm were recovered in 4 of 25 cases using mTESE.Conclusions: MMTSE shows promise as a simple method that comprehensively searches testicular tissue and retrieves sperm using an appropriate method while minimizing patient burden.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12566"},"PeriodicalIF":2.7,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic and microbiome analysis of cervicovaginal mucus in in vitro fertilization-embryo transfer: Toward predicting pregnancy success. 体外受精-胚胎移植中宫颈阴道粘液的代谢组和微生物组分析：预测妊娠成功率。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-03-11 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12568

Eiji Nishio, Aya Iwata, Rie Kawasaki, Kukimoto Iwao, Haruki Nishizawa, Takuma Fujii

{"title":"Metabolomic and microbiome analysis of cervicovaginal mucus in in vitro fertilization-embryo transfer: Toward predicting pregnancy success.","authors":"Eiji Nishio, Aya Iwata, Rie Kawasaki, Kukimoto Iwao, Haruki Nishizawa, Takuma Fujii","doi":"10.1002/rmb2.12568","DOIUrl":"10.1002/rmb2.12568","url":null,"abstract":"Purpose: In the context of in vitro fertilization-embryo transfer (IVF-ET), factors other than egg quality may be key determinants of treatment success, in particular, maternal factors related to uterine endometrial receptivity and unidentified factors. We therefore aimed to analyze the metabolome and microbiome in IVF-ET patients who did and did not achieve pregnancy.Methods: Cervicovaginal mucus was collected from patients undergoing IVF-ET. Metabolite analysis was conducted by liquid chromatography-mass spectrometry and the microbiota were determined by the polymerase chain reaction using universal 16S-rRNA gene bacterial primers by MiSeq sequencing. Patients were classified as pregnant (N = 10) or nonpregnant (N = 13). Metabolic pathways were examined by MetaboAnalyst.Results: Three metabolic pathways, including alanine-aspartate-glutamate metabolism, arginine biosynthesis, and cysteine-methionine metabolism, were commonly decreased at the time of embryo transfer irrespective pregnant outcomes. Notably, pyruvate was decreased in the pregnant group. Amino acid metabolites showed inverse correlations with the presence of anaerobic microbiota in the nonpregnant group.Conclusions: Metabolism decreased during embryo transplantation, with a notable decrease in pyruvate metabolism, particularly in patients who became pregnant. The behavior of metabolites in the pregnant and nonpregnant groups suggests that metabolome analysis in the cervicovaginal mucus may be a diagnostic marker for predicting pregnancy.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12568"},"PeriodicalIF":2.7,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The number of previous implantation failures is a critical determinant of intrauterine autologous platelet-rich plasma infusion success in women with recurrent implantation failure. 对于反复种植失败的妇女来说，之前种植失败的次数是决定宫内自体富血小板血浆输注成功与否的关键因素。

IF 2.7 3区医学

Reproductive Medicine and Biology Pub Date : 2024-02-29 eCollection Date: 2024-01-01 DOI: 10.1002/rmb2.12565

Shunsaku Fujii, Takaaki Oguchi

{"title":"The number of previous implantation failures is a critical determinant of intrauterine autologous platelet-rich plasma infusion success in women with recurrent implantation failure.","authors":"Shunsaku Fujii, Takaaki Oguchi","doi":"10.1002/rmb2.12565","DOIUrl":"10.1002/rmb2.12565","url":null,"abstract":"Purpose: We aimed to identify factors influencing the reproductive outcomes of frozen-thawed embryo transfer (FET) with intrauterine autologous platelet-rich plasma (PRP) infusion in patients with either a thin endometrium or recurrent implantation failure (RIF) despite a normal endometrial appearance.Methods: In this retrospective study of women who underwent PRP-FET, factors influencing PRP-FET outcomes were identified using multivariate logistic regression analysis.Results: We enrolled 111 patients (70 with refractory thin endometrium and 41 with RIF but no thin endometrium). For 99 completed FET cycles, the β-hCG positivity rate was 46.7%, clinical pregnancy rate (CPR) was 41.0%, and live birth rate (LBR) was 36.2%. PRP treatment was associated with significant improvements over previous cycles, and participants with thin endometria demonstrated thickening. Multivariate logistic regression analysis showed that the number of previous implantation failures in women with RIF was a significant factor affecting the PRP-FET outcomes. The CPR and LBR of women with RIF were lower when there had been ≥3 previous implantation failures occurred.Conclusions: Intrauterine PRP infusion improves the pregnancy outcomes of patients with RIF or a thin endometrium. The number of previous implantation failures is a critical determinant of successful intrauterine PRP infusions in women with RIF.","PeriodicalId":21116,"journal":{"name":"Reproductive Medicine and Biology","volume":"23 1","pages":"e12565"},"PeriodicalIF":2.7,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10904764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140022537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0