The effect of ionizing radiation on testicular interstitial stromal cells.

IF 2.7 3区医学 Q2 OBSTETRICS & GYNECOLOGY

Reproductive Medicine and Biology Pub Date : 2025-03-19 eCollection Date: 2025-01-01 DOI:10.1002/rmb2.12639

Esraa Tamam, Chen Yan, Reiko Sekiya, Tao-Sheng Li

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引用次数: 0

Abstract

Purpose: Testis is one of the most radiosensitive tissues. Interstitial stromal cells play a supportive role in male fertility, but radiation-induced damage to those cells has not yet been well understood. We aimed to investigate radiation-induced changes in interstitial stromal cells in the testis.

Methods: Adult male C57BL/6N mice (8 weeks) received a single pelvic exposure to a relatively high dose (1 Gy) or a very high dose (8 Gy) X-ray. We collected the testicular tissues for evaluation at 1, 9, and 60 days after irradiation.

Results: We detected a recoverable moderate degeneration of seminiferous tubules after 1 Gy exposure but an irreversible severe damage to the testis after 8 Gy exposure. Immunostaining results revealed that 1 Gy exposure induced DNA damage at day 1, upregulated intratubular GDNF at days 1 and 9, upregulated FGF at all time points, and upregulated CSF-1R at day 9. In contrast, 8 Gy exposure induced DNA damage at days 1 and 9, upregulated intratubular GDNF at days 1 and 9, downregulated CD105 at day 60, and upregulated FGF at all time points.

Conclusion: Radiation-induced dynamic changes to interstitial stromal cells in the testis. Upregulated interstitial CSF-1R and FGF2 may support spermatogenesis recovery after high-dose radiation.

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电离辐射对睾丸间质细胞的影响。

目的：睾丸是放射最敏感的组织之一。间质细胞在男性生育能力中起支持作用，但辐射对这些细胞造成的损伤尚未得到很好的了解。我们的目的是研究辐射引起的睾丸间质细胞的变化。方法：成年雄性C57BL/6N小鼠（8周）单次骨盆照射相对高剂量（1 Gy）或甚高剂量（8 Gy） x射线。我们在照射后1、9和60天收集睾丸组织进行评估。结果：我们检测到1 Gy暴露后可恢复的精管中度变性，但8 Gy暴露后对睾丸造成不可逆的严重损伤。免疫染色结果显示，1 Gy暴露在第1天诱导DNA损伤，在第1天和第9天上调小管内GDNF，在所有时间点上调FGF，并在第9天上调CSF-1R。相比之下，8gy暴露在第1天和第9天诱导DNA损伤，在第1天和第9天上调小管内GDNF，在第60天下调CD105，并在所有时间点上调FGF。结论：辐射引起睾丸间质细胞的动态变化。间质CSF-1R和FGF2的上调可能支持高剂量辐射后精子发生的恢复。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive Medicine and Biology Multiple-

CiteScore

5.70

自引率

5.90%

发文量

审稿时长

20 weeks

期刊介绍： Reproductive Medicine and Biology (RMB) is the official English journal of the Japan Society for Reproductive Medicine, the Japan Society of Fertilization and Implantation, the Japan Society of Andrology, and publishes original research articles that report new findings or concepts in all aspects of reproductive phenomena in all kinds of mammals. Papers in any of the following fields will be considered: andrology, endocrinology, oncology, immunology, genetics, function of gonads and genital tracts, erectile dysfunction, gametogenesis, function of accessory sex organs, fertilization, embryogenesis, embryo manipulation, pregnancy, implantation, ontogenesis, infectious disease, contraception, etc.