Reproduction in Domestic Animals最新文献

{"title":"MitoQ Preserves Epigenetic Modifications and Quality Parameters of Rooster Sperm During Cryopreservation Process.","authors":"Parisa Alipour-Jenaghard, Hossein Daghigh-Kia, Reza Masoudi, Ali Hatefi","doi":"10.1111/rda.70012","DOIUrl":"10.1111/rda.70012","url":null,"abstract":"<p><p>Cryopreservation in rooster semen is a helpful procedure to spread qualified semen samples for reproductive goals. Nevertheless, some post-thawed qualified semen samples showed a considerably poor fertility rate that might be related to epigenetic modifications during the cryopreservation process. This study aims to investigate the effect of the cryopreservation process in the presence of MitoQ as a mitochondrial-targeted antioxidant on epigenetic changes and other quality parameters (motility, morphology, mitochondrial activity, acrosome integrity, lipid peroxidation, DNA fragmentation, apoptosis status, and ROS concentration) of rooster sperm. The collected semen samples were divided into four groups of fresh samples and three groups that were supplemented by MitoQ 0, 10, and 100 nM and cryopreserved. The cryopreservation process reduced (p ≤ 0.05) DNA methylation, H3K9 acetylation, H3K4 methylation, motility parameters, membrane integrity, mitochondrial activity, acrosome integrity, viability, and increased (p ≤ 0.05) lipid peroxidation, DNA fragmentation, ROS concentration, and apoptotic-like changes compared to the fresh semen group. However, in frozen sperm groups, MitoQ 10 and 100 nM resulted in significant improvements (p ≤ 0.05) in the epigenetic modifications and other mentioned quality parameters compared to the control group (MitoQ 0). Generally, although the cryopreservation process reduced semen quality, using MitoQ could be useful in cryopreserved rooster semen quality.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 2","pages":"e70012"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Müllerian Hormone Concentrations Can Be Reliably Determined by a Single Measurement, Irrespective of Cycle, in Synchronised Ewes During Non-Breeding Season.","authors":"Nebi Cetin, Davut Koca","doi":"10.1111/rda.70010","DOIUrl":"10.1111/rda.70010","url":null,"abstract":"<p><p>Improvement of yield characteristics in animal breeding is important in terms of increasing animal production and sustainability. Fertility is one of the most important yield traits affecting economic gain in sheep breeding. Anti-Müllerian Hormone (AMH) is widely recognised as a dependable biomarker for assessing ovarian reserves and fertility potential. The aim of this study was to evaluate the dynamics of AMH during different phases of the sexual cycle in Norduz ewes the non-breeding season. Additionally, the study sought to assess the effects of age and body condition score (BCS) on AMH concentrations during these phases. A total of 32 Norduz ewes with a body condition score (BCS) of 3-4.5 and aged between 2 and 4 years were used as animal material in the study. All experimental procedures were carried out outside the breeding season and when the ewes were lactating. In all ewes in anestrus, intravaginal sponges (Esponjavet, 60 mg MAP, Hipra, Turkey) were kept in the vagina for 7 days for estrus synchronisation. Intramuscular injections of PMSG (Oviser, 500 IU, Hipra, Turkey) and PGF2α analog (Gestavet, 50 μg, Hipra, Turkey) were administered 48 h prior to sponge removal. Twenty-four hours after sponge removal, ewes were exposed to the ram for estrus detection. Since 5 ewes did not show estrus, blood samples were collected regularly from animals (n = 27) in which estrus was detected at three different stages: one just before the insertion of vaginal sponges (anestrus), another when heat was detected exposing to the ram (estrus), and the final one 10 days after estrus (diestrus). The serum samples were assessed for the levels of AMH and progesterone through the electrochemiluminessence immunoassay technique (ECLIA). The results of the analyses showed that serum AMH concentration did not vary between anestrus, estrus and diestrus phases of the sexual cycle of Norduz ewes outside the breeding season (p > 0.05). Furthermore, age and BCS had no effect on progesterone and AMH levels in different phases of the sexual cycle (p > 0.05). In conclusion, this study shows that serum AMH levels are constant at any stage of the estrus cycle. This suggests that phenotypic evaluation of ewes can be performed with a single measurement and that AMH is a reproducible and dependable biomarker that can be measured at any stage of the estrus cycle at an arbitrary time point.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 2","pages":"e70010"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Effect of Asprosin Level on Fertility Success and Oxidative Stress in Simmental Cows.","authors":"Rahmi Köse, Serkan Ali Akarsu, Serdal Kurt, Hakan Serin, Mehmet Akif Aydın, Ahmet Karahanlı, Gamze Uçak, Ahmet Yörü","doi":"10.1111/rda.70022","DOIUrl":"10.1111/rda.70022","url":null,"abstract":"<p><p>Asprosin, a protein hormone that regulates hunger and glucose homeostasis, is produced by white adipose tissue. The aim of this study was to investigate the effect of asprosin level on fertility and oxidative stress parameters in Simmental cows. In the study, 44 Simmental multiparous cows were used. PRID was used to synchronise the animals. Blood samples were obtained from the cattle on the day of artificial insemination, and the amount of asprosin was measured. Animals were divided into two groups: a low asprosin (LA) group and a high asprosin (HA) group. On day 25, blood samples were taken, and pregnancy-associated glycoprotein (PAG2), total antioxidant level (TAS) and total oxidant level (TOS) were measured using commercial kits. Ultrasonography was used to diagnose pregnancy on day 45. The TAS level at day 25 was higher in the HA group than the TAS level at the beginning of the study (p = 0.007). The TOS level at the beginning of the study was higher in the HA group than in the LA group (p = 0.017). The TOS level of cows in the LA group on day 25 was significantly higher than the TOS level at the beginning of the study (p = 0.028). In conclusion, asprosin levels in cows affected oxidative stress parameters. According to receiver operating characteristic curve (ROC) analysis, there was a strong correlation between TOS and asprosin, and it was concluded that cows with HA values may be exposed to oxidative stress.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 2","pages":"e70022"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Electroporation Timing and Cumulus Cell Attachment on In Vitro Development and Genome Editing of Porcine Embryos.","authors":"Nanaka Torigoe, Qingyi Lin, Bin Liu, Yuichiro Nakayama, Aya Nakai, Megumi Nagahara, Fuminori Tanihara, Maki Hirata, Takeshige Otoi","doi":"10.1111/rda.70011","DOIUrl":"10.1111/rda.70011","url":null,"abstract":"<p><p>Pig genome editing using the oviductal nucleic acid delivery (GONAD) method with electroporation would allow the efficient obtention of genetically modified pigs. However, oocytes and zygotes at various stages after ovulation must be targeted, and cumulus cell attachment and mosaic mutations are major obstacles. Therefore, we investigated whether two parameters (electroporation timing and the cumulus cell attachment) influence the effectiveness of multiplex genome editing by electroporation in porcine oocytes or zygotes. Three gRNAs targeting either GGTA1, CMAH or B4GALNT2 were introduced individually into oocytes and zygotes with and without cumulus cells at three different time points, 0 h before in vitro fertilisation (IVF) and 5 h and 10 h after IVF initiation. The introduction of gRNAs into oocytes and zygotes did not significantly affect the rates of blastocyst formation and total mutation of the resulting blastocysts irrespective of cumulus cell attachment and electroporation timing. In conclusion, the electroporation timing and the cumulus cell attachment did not interfere with the efficient delivery of the CRISPR/Cas9 system to the oocytes/zygotes, indicating that porcine genome editing in the oviduct using GONAD method may be possible.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 2","pages":"e70011"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardisation of Dog Sperm Morphology Classification.","authors":"Leonardo F C Brito, Marco C da Silva, Kara A Kolster","doi":"10.1111/rda.70024","DOIUrl":"10.1111/rda.70024","url":null,"abstract":"<p><p>Sperm morphology is an important component of dog fertility analysis. Visual examination is the gold standard technique for evaluation, but large variations within and across evaluators have been documented. Databases of sperm morphology images are valuable practical aids for training and performance testing and can improve sperm classification standardisation; however, such databases are only useful when the material is expertly classified to serve as a reference. The objective of this study was to build an expertly annotated database of sperm images and to generate an illustrated guide with examples of the different sperm morphology presentations to promote standardisation in dog sperm morphology classification. Semen samples (n = 42) were obtained either from dogs presented for fertility evaluation or from semen doses used for artificial insemination. For image acquisition, wet preparations were prepared and evaluated using phase-contrast optics under 1000× magnification. Selected images were cropped so that only one sperm was included in each file, and a database of 8817 images was created. The images were classified by three veterinarians, specialists in animal reproduction (Diplomates American College of Theriogenologists) according to different criteria. Agreement among evaluators was the criterion used to create a database with reconciled classification. Complete agreement on defect category and morphological classification was observed on 70.2% of the images, and at least two veterinarians agreed on the classification of another 27.4% of the images; therefore, the reconciled database classification was based on the consensus of at least two veterinarians on 97.5% of the images. The percentage of morphologically normal sperm in the reconciled database was 57%, with the primary defect involving the sperm head, midpiece, or tail in 18%, 20%, and 5% of the cases, respectively. Among defective sperm, 39% had a single defect and 4% had multiple defects. When the total prevalence of specific defects was computed, the reconciled database contained 18% sperm head abnormalities, 22% midpiece abnormalities, and 7% tail abnormalities. Comprehensive illustrations of the variation in normal and abnormal dog sperm morphology from the database built based on reconciled expert classification are provided for reference.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 2","pages":"e70024"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11848706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Mechanism of N-Acetylcysteine Regulating Proliferation and Hormone Secretion of Granulosa Cells in Sheep.","authors":"Jine Wang, Yahua Yang, Junyuan Ma, Pinshen Li, Yang Li, Chongfa Yang, Binzhu Zhao, Zhijie Zhao, Xiaona Ding, Hao Chen, Shengdong Huo, Yanmei Yang, Wenxue Luo","doi":"10.1111/rda.70006","DOIUrl":"10.1111/rda.70006","url":null,"abstract":"<p><p>Granulosa cells (GCs) are pivotal in the development of ovarian follicles, serving not only as supportive cells but also as the primary producers of steroid hormones. The proliferation of these cells and the synthesis of steroid hormones are crucial for follicular development and atresia. In our study, GCs were isolated using follicular fluid aspiration and subsequently identified through immunofluorescence. We investigated the impact of varying concentrations of N-acetylcysteine (NAC) at 50, 100, 500, and 1000 μmol/L on sheep GCs, focusing on antioxidant levels, proliferation, apoptosis, and steroid hormone secretion. The phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) inhibitor LY294002 was used to explore the molecular mechanism of NAC on GCs proliferation and steroid hormone secretion in sheep. Our findings indicate that all concentrations of NAC tested promoted GC proliferation and suppressed apoptosis in sheep GCs. Notably, 100 μmol/L NAC exhibited the most pronounced effect on GC proliferation after 48 h. The expression levels of CCND1, CDK4, and Bcl-2 were significantly elevated in all NAC concentration groups, whereas Bax expression was notably reduced. Furthermore, all NAC concentrations led to a significant reduction in reactive oxygen species (ROS) levels and an increase in the expression of CAT and SOD1. NAC also significantly enhanced the expression of CYP19A1 and 3β-HSD, as well as the secretion of estradiol (E2) and progesterone (P4) by GCs. In summary, NAC activates the PI3K/AKT signalling pathway, thereby promoting the proliferation of GCs and the secretion of E2 and P4 by sheep GCs in vitro.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 1","pages":"e70006"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Spectral Doppler Ultrasound in Selecting an Equine Embryo Receiver\".","authors":"","doi":"10.1111/rda.70002","DOIUrl":"https://doi.org/10.1111/rda.70002","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 1","pages":"e70002"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Equol Alleviates the In Vitro Aging-Induced Disruption of Porcine Oocytes.","authors":"Rui Chen, Runtian Wan, Ke Peng, Xinxin Liu, Benliang Zhou, Rijing He, Yujun Yan, Yanan Zhao, Ye-Shi Yin, Huiyan Xu, Xiaogan Yang, Xingwei Liang","doi":"10.1111/rda.70007","DOIUrl":"10.1111/rda.70007","url":null,"abstract":"<p><p>Oocyte quality is crucial for determining the subsequent embryo developmental capacity and reproductive outcomes. However, aging is detrimental to oocyte quality. Previous studies have demonstrated that soy isoflavones have positive effects on the reproductive performance of female pigs. Equol, the primary metabolite of soy isoflavones, is renowned for its antioxidant properties and its ability to scavenge reactive oxygen species (ROS). However, the potential role of equol in reversing aging-mediated oocyte decline has not yet been elucidated. In this study, we treated the porcine oocytes with different concentrations of equol (2.5, 5 and 10 μM) during prolonged in vitro culture. Our findings showed that aging led to decreased embryonic developmental capacity, indicating the decline of oocyte quality. We further found that aging disrupted spindle assembly and chromosome arrangement, impaired actin polymerisation and reduced mitochondrial activity and function. Moreover, aging increased ROS levels; thereafter, DNA damage and apoptosis was induced in the porcine oocytes. Interestingly, treatment with 2.5 μM equol during the aging process significantly mitigated the above-mentioned defective parameters in porcine oocytes and finally improved embryo development rates. Collectively, these results imply that equol has potential benefits in attenuating the aging-mediated defects on porcine oocytes.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 1","pages":"e70007"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asynchronous Embryo Transfer in Ewes.","authors":"Nana Yang, Yiyong Liu, Jing Zhang, Shuyuan Sun, Xinglong Wu, Xiangyun Li","doi":"10.1111/rda.70004","DOIUrl":"https://doi.org/10.1111/rda.70004","url":null,"abstract":"<p><p>The aim of the present study was to evaluate the effects of spatially and/or temporally asynchronous transfer of in vivo embryos at different stages in ewes during the breeding season. Four experiments were carried out. In Experiment 1, 207 blastocysts that had been frozen and thawed were transferred into the oviducts of 43 day two recipients, the oviducts of 23 day six recipients, and the uteri of 141 day six recipients, respectively. In Experiment 2, 287 fresh blastocysts were transferred into the oviducts of 26 day six recipients and the uteri of 261 day six recipients, respectively. In Experiment 3, 104 2-cell stage embryos were transferred into the oviducts of 80 day two recipients and the uteri of 24 day two recipients, respectively. In Experiment 4, 175 8-cell stage embryos were transferred into the oviducts of 160 day three recipients and the uteri of 15 day three recipients, respectively. The results showed that spatially asynchronous but temporally synchronous uterine transfers of fresh cleavage-stage embryos or oviductal transfers of fresh blastocysts did not compromise pregnancy and lambing. Furthermore, the oviductal transfers of frozen blastocysts significantly increased the pregnancy rate compared with uterine transfers of frozen blastocysts. Additionally, it is worth noting that the oviduct may be the most suitable microenvironment for frozen embryos in ewes. More studies should be conducted to assess the effects of asynchronous embryo transfer in ewes in a large sample size.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 1","pages":"e70004"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142954136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic Antioxidants and Their Differential Response to Cryopreservation in Japanese Quail Semen.","authors":"Sumiyyah Zuha, Bushra Allah Rakha, Shamim Akhter","doi":"10.1111/rda.70005","DOIUrl":"10.1111/rda.70005","url":null,"abstract":"<p><p>A triad of enzymatic antioxidants viz., catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) constitutes a first line of defence against any redox imbalances in the semen. Cryopreservation enabling long term storage of semen also prompts generation of surplus reactive oxygen species (ROS) in the cells with waned antioxidants, hampering the full exploitation of this process. With Japanese quail having all the potential to satisfy the steeply growing poultry demand, there is a need for its propagation via sperm preservation and assisted reproduction. Therefore, current study aimed at investigating enzymatic antioxidants in the Japanese quail semen, level of decline in their activity during cryopreservation and any intra-species variation in their levels. Moreover, malonaldehyde (MDA) levels in fresh and cryopreserved semen of individual birds were taken into account. Semen was collected separately from seven mature males, diluted with NaCl extender (1:15) and cryopreserved. Analysis of sperm and seminal plasma was done at fresh collection, post-dilution, post-cooling, post-equilibration and post-thaw stages of freezing. The results confirmed the presence of CAT, SOD and GPx in fresh sperm and seminal plasma, their decline (p < 0.05) at all stages of cryopreservation and intra-species variation (p < 0.05) with respect to freeze-tolerance. Furthermore, significant increase was recorded in MDA levels at varying rates among individual quails during semen cryopreservation. In conclusion, Japanese quail sperm and seminal plasma exhibit enzymatic antioxidant activities to monitor ROS generation and performance of these antioxidants is severely affected by freezing process with intra-species variation and rise (p < 0.05) of lipid peroxidation (LPO).</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 1","pages":"e70005"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143034102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}