Reproduction in Domestic Animals最新文献_第10页

Humanin as a Molecular Indicator of Semen Quality and Cryotolerance in Crossbred Cattle. 人素作为杂交牛精液质量和低温耐受性的分子指标。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-06-01 DOI: 10.1111/rda.70240

Amare Eshetu Gemeda, Mahak Singh, Rahul Katiyar, Abhishek Kumar, Megha Pande, M K Patra, Karikalan Mathesh, N Srivastava, S K Ghosh

{"title":"Humanin as a Molecular Indicator of Semen Quality and Cryotolerance in Crossbred Cattle.","authors":"Amare Eshetu Gemeda, Mahak Singh, Rahul Katiyar, Abhishek Kumar, Megha Pande, M K Patra, Karikalan Mathesh, N Srivastava, S K Ghosh","doi":"10.1111/rda.70240","DOIUrl":"10.1111/rda.70240","url":null,"abstract":"The present study aimed to assess the presence of humanin in the sperm of crossbred cattle bulls using immunocytochemistry and to evaluate its potential as a semen quality marker. For this, a total of 49 ejaculates were collected from three Vrindavani crossbred bulls and were evaluated for volume, individual progressive motility (IPM), abnormality, and sperm concentration. The ejaculates were grouped into four categories, that is normozoospermia, oligozoospermia, asthenozoospermia and oligoasthenozoospermia, based on sperm concentration and IPM. Furthermore, normozoospermic ejaculates were cryopreserved and retrospectively subdivided into two groups (good-freezable and poor-freezable) based on post-thaw motility (PTM). Immunofluorescence assays revealed humanin in the neck and upper midpiece regions of sperm. Humanin levels in seminal plasma were significantly higher (p < 0.05) in normozoospermic individuals than in other groups. Humanin levels in seminal plasma were positively and significantly correlated with sperm concentration and individual progressive motility (p < 0.05). Out of 28 normozoospermia ejaculates, 19 ejaculates (67.86%) were categorized as good freezable, and nine ejaculates (32.14%) were grouped as poor freezable. Humanin level in the seminal plasma of good freezable ejaculates was significantly (p < 0.05) higher than that of poor freezable ejaculates. In conclusion, our research demonstrated, the presence of the humanin peptide in the sperm and seminal plasma of Vrindavani bulls. Additionally, the role of humanin as a potential semen-quality marker in crossbred bull semen was established to identify high-quality, freezeable ejaculates.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 6","pages":"e70240"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of miR-105-1, miR-152, Vinculin and α-Parvin in the Control of Porcine Ovarian Cell Functions. Do miRNAs and siRNAs Need a Transfection Reagent? miR-105-1、miR-152、Vinculin和α-Parvin参与猪卵巢细胞功能的调控mirna和sirna需要转染试剂吗？

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-05-01 DOI: 10.1111/rda.70219

Alexander V Sirotkin, Zuzana Fabová, Barbora Loncová, Vladimíra Mondočková, Radoslav Omelka, Abdel Halim Harrath

{"title":"Involvement of miR-105-1, miR-152, Vinculin and α-Parvin in the Control of Porcine Ovarian Cell Functions. Do miRNAs and siRNAs Need a Transfection Reagent?","authors":"Alexander V Sirotkin, Zuzana Fabová, Barbora Loncová, Vladimíra Mondočková, Radoslav Omelka, Abdel Halim Harrath","doi":"10.1111/rda.70219","DOIUrl":"10.1111/rda.70219","url":null,"abstract":"The aim of the present study was to examine the role of miR-105-1, miR-152, vinculin (VCL) and α-parvin (PARVA) in the control of ovarian cell functions, and whether miRNAs and siRNAs could enter and affect cultured cells with and without the application of carriers. For this purpose, we (1) examined the incorporation, expression and effect of the selected miRNAs (miR-105-1 and miR-152 mimics and antagonists) and siRNAs downregulating VCL and PARVA on cultured porcine ovarian granulosa cells; and (2) compared the incorporation, expression, and effects of these miRNAs and siRNAs used without and with carriers (transfection reagent: Lipofectamine). The incorporation and expression of these RNAs, viability, proliferation, apoptosis and steroid hormone release of cells were analysed by immunocytochemistry, RT-qPCR, the alamarBlue test, BrdU incorporation, a DNA fragmentation (Cell Death Detection) kit and ELISA. The obtained results demonstrate (1) that miR-105-1, miR-152, VCL and PARVA can be stimulators of the main basic ovarian cell functions; (2) that miRNAs can be incorporated and expressed in ovarian cells and affect their functions without a carrier; and (3) that a carrier is not necessary for siRNA incorporation and expression but is necessary for their influence on ovarian cell functions.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70219"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ProAKAP4: A Promising Biomarker Linking Post-Thaw Sperm Quality to Fertility in Dogs? ProAKAP4：狗解冻后精子质量与生育能力相关的生物标志物？

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-05-01 DOI: 10.1111/rda.70218

Djemil Bencharif, Rédha Belala, Nora Mimoune, Dolorès Le Couazer, Morgane Le-Guiriec, Mouna Chakchouk, Lucas Teixeira Fangueiro, Hamed Farnia

{"title":"ProAKAP4: A Promising Biomarker Linking Post-Thaw Sperm Quality to Fertility in Dogs?","authors":"Djemil Bencharif, Rédha Belala, Nora Mimoune, Dolorès Le Couazer, Morgane Le-Guiriec, Mouna Chakchouk, Lucas Teixeira Fangueiro, Hamed Farnia","doi":"10.1111/rda.70218","DOIUrl":"10.1111/rda.70218","url":null,"abstract":"ProAKAP4, the precursor of A-kinase anchoring protein 4 (AKAP4), is a sperm-specific structural protein involved in flagellar organization, regulation of motility and energy metabolism. It has emerged as a potential biomarker of semen quality and fertility in several mammalian species. However, its relevance in canine reproduction remains insufficiently characterized. This study investigated the association between proAKAP4 concentrations, sperm kinematic parameters and fertility outcomes in dogs. Twenty-six ejaculates were collected from eight privately owned dogs (5-11 years old, various breeds), cryopreserved and evaluated before and after thawing. Sperm motility and velocity parameters were assessed using Computer-Assisted Sperm Analysis (CASA). ProAKAP4 concentrations were quantified using a species-Specific Dog 4MID ELISA assay. Artificial inseminations were performed in six bitches, and pregnancy rates and litter sizes were recorded. Post-thaw samples showed higher measurable proAKAP4 concentrations than fresh semen. However, no significant correlations were observed between proAKAP4 levels and total motility, progressive motility, conception rate, or litter size (p < 0.05) concentrations and sperm velocity parameters (VAP, VSL and VCL). These findings suggest that proAKAP4 may reflect aspects of sperm structural integrity.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70218"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148006564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of Single Nucleotide Polymorphism Ss52050737 in Luteinizing Hormone/Choriogonadotropin Receptor Gene on Some Reproductive Traits in Friesian Cows. 黄体生成素/绒毛膜促性腺激素受体基因单核苷酸多态性Ss52050737对奶牛生殖性状的影响

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-05-01 DOI: 10.1111/rda.70212

Samar Samir Youssef, Eman Abd El-Razek Abbas, Ahmed S A Sosa, Mahmoud H Hasanain, Sally Ibrahim, Mohamed M M El-Sokary, Mahmoud F Nawito, Karima Gh M Mahmoud

{"title":"The Impact of Single Nucleotide Polymorphism Ss52050737 in Luteinizing Hormone/Choriogonadotropin Receptor Gene on Some Reproductive Traits in Friesian Cows.","authors":"Samar Samir Youssef, Eman Abd El-Razek Abbas, Ahmed S A Sosa, Mahmoud H Hasanain, Sally Ibrahim, Mohamed M M El-Sokary, Mahmoud F Nawito, Karima Gh M Mahmoud","doi":"10.1111/rda.70212","DOIUrl":"10.1111/rda.70212","url":null,"abstract":"The luteinizing hormone/choriogonadotropin receptor (LHCGR) gene is potentially a useful genetic marker for cattle reproductive status. This study aimed to investigate the impact of the LHCGR gene's ss52050737 polymorphism on age at first calving (AFC), calving interval (CI), and ovarian status using the TaqMan PCR assay. AFC was categorized into 25-34 and 35-45 months, while CI was grouped into 330-410 and 411-800 days. Ovarian status was classified as active or inactive ovaries. Blood samples were collected from cows using EDTA-containing vacutainer tubes and stored at -20°C until DNA extraction and analysis. Three genotypes (GG, GT, and TT) were identified for the LHCGR gene SNP (ss52050737). The homozygous GG genotype was more frequent in cows with a CI of 330-410 days, and the genotype distribution showed a statistically significant association (p = 0.018) in cows with CI 330-410 and 411-800 days. On the other hand, the GG genotype was not significantly different in relation to AFC and ovarian status (active or inactive). These findings suggest that the ss52050737 polymorphism of the LHCGR gene has an impact on calving interval, and the G allele is associated with the CI of 330-410 days.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70212"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overcoming Cryopreservation Challenges in Mammalian Sperm: Strategies for Mitigating Cryoinjuries. 克服哺乳动物精子低温保存的挑战：减轻低温损伤的策略。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-05-01 DOI: 10.1111/rda.70214

Allai Larbi, Chunyan Li, Badaoui Bouabid, Guoquan Wu, Guobo Quan

{"title":"Overcoming Cryopreservation Challenges in Mammalian Sperm: Strategies for Mitigating Cryoinjuries.","authors":"Allai Larbi, Chunyan Li, Badaoui Bouabid, Guoquan Wu, Guobo Quan","doi":"10.1111/rda.70214","DOIUrl":"10.1111/rda.70214","url":null,"abstract":"Cryopreservation is a critical tool in the livestock industry, particularly for disseminating genetic materials and establishing sperm banks to preserve species. Developing a successful semen storage procedure is essential to minimize the negative effects caused by the cryopreservation process. Mammalian sperm are highly susceptible to cryoinjury and primarily inflicted during the freezing and thawing processes when extracellular water freezes and intracellular water is lost, negatively impacting sperm quality parameters and fertility. Cryoinjuries generally cause irreversible damage to sperm structure and function. Currently, various cryopreservation measures, such as selection of cryoprotectants and optimization of freezing and thawing processes, are employed to reduce sperm cryoinjuries and to elucidate the mechanisms underlying cryodamage. However, the exact nature of sperm cryoinjury remains largely unclear. This review discusses the current understanding of sperm cryodamage induced by the cryopreservation process, factors influencing sperm susceptibility to cryoinjury, the impacts of cryopreservation on proteins, metabolites, lipids, and RNAs. As one of the important measures to mitigate sperm cryoinjuries, the application of antioxidants is becoming increasingly important. So, the effects of antioxidants on post-thaw sperm quality are reviewed here.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70214"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laparoscopic Embryo Transfer in Swine: An Effective Alternative to Traditional Open Surgery. 猪腹腔镜胚胎移植：传统开放手术的有效替代。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-05-01 DOI: 10.1111/rda.70220

H Baldassarre, L G Currin, M P de Macedo, V Guay, F L Facioli, R Duffy, A Ojerio, L Socransky, Z da Silva, K Gutierrez, W G Glanzner, V Bordignon

{"title":"Laparoscopic Embryo Transfer in Swine: An Effective Alternative to Traditional Open Surgery.","authors":"H Baldassarre, L G Currin, M P de Macedo, V Guay, F L Facioli, R Duffy, A Ojerio, L Socransky, Z da Silva, K Gutierrez, W G Glanzner, V Bordignon","doi":"10.1111/rda.70220","DOIUrl":"10.1111/rda.70220","url":null,"abstract":"While embryo transfer (ET) has not yet been widely adopted in the commercial swine industry, it is an essential tool in biomedical research, including the creation of gene-edited pigs. Traditionally, ET in pigs is performed by open surgery (laparotomy), an effective but invasive and time-consuming method associated with risks such as infection and herniation. To overcome these limitations, we developed a minimally invasive laparoscopic embryo transfer (LapET) method enabling ET into the oviduct using a sheep insemination device (Aspic, IMV Technologies, France). Seventeen gilts were synchronised with 17.6 mg altrenogest daily for 16 days, 10 mg dinoprost on D15, 1250 IU eCG on D16 and 1000 IU hCG on D19, followed by LapET on D21. Each gilt received an average of 160 embryos, transferred 24-48 h post-somatic cell nuclear transfer (SCNT). Under general anaesthesia, gilts were placed on a surgical table in a 30° incline Trendelenburg position. Three trocars were inserted to serve as ports for the laparoscope, a grasper and the Aspic pipette. The grasper served to expose the ovaries to confirm ovulation and to position the oviduct for embryo deposition. Following ET, the oviduct was released, instruments were removed and incisions were closed with surgical glue. Pregnancy was confirmed by ultrasonography, with 12 of 17 gilts (70.6%) diagnosed as pregnant, a success rate comparable to our recent laparotomy-based SCNT ETs into the oviduct (71.4%; 10 of 14). These results demonstrate that LapET is a viable, fast, minimally invasive and effective alternative to traditional open surgery ET in swine.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70220"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13155260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147842148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Resolution Optical Microscopy Technique Aplicated to Study of Placenta of Mummificated Foetuses in Swine. 高分辨光学显微镜技术在猪木乃伊化胎儿胎盘研究中的应用。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-05-01 DOI: 10.1111/rda.70234

A L Cristofolini, M R Fiorimanti, K P Gomez, T Porcel de Peralta, C G Barbeito, C I Merkis

{"title":"High Resolution Optical Microscopy Technique Aplicated to Study of Placenta of Mummificated Foetuses in Swine.","authors":"A L Cristofolini, M R Fiorimanti, K P Gomez, T Porcel de Peralta, C G Barbeito, C I Merkis","doi":"10.1111/rda.70234","DOIUrl":"10.1111/rda.70234","url":null,"abstract":"In the sows, fetal mortality that occurs between Day 35 and term, causes on average the loss of more than one piglet per litter. During prenatal development, the ossification process begins very early in the fetal stage, where dead foetuses are not reabsorbed, but rather undergo a mummification process. The objective was to analyze and compare the porcine placental morphostructure of mummified foetuses and of normal placentation, through the application of high-resolution optical microscopy (HRLM). The placental tissues were collected immediately after delivery, was fixed in glutaraldehyde and processed by HRLM. Semi-thins sections were counterstained with toluidina's blue o with azur II and were observed in a light microscope. The analysis showed that the placentas of mummified foetuses have a different histoarchitecture than placentas from healthy foetuses. In the mesenchyme, the blood vessels showed different size, arrangement and morphological characteristics. Numerous capillaries arranged around areolas and venules were observed. Microthrombus formation was not observed. Cytoplasmic vacuolization, chromatolysis and cell detachment were observed in the trophoblastic epithelium. These all characteristics correspond to postmortem morphological changes. The HRLM allows the structural morphological evaluation and the digital morphometric analysis, with high definition and greater resolution. We consider that HRLM is a relevant tool that allows us to maximize the use of light field microscopy.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70234"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Proteomic Approaches in Sperm Sorting: Shaping the Future of Reproductive Biotechnology. 精子分选中的新兴蛋白质组学方法：塑造生殖生物技术的未来。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-05-01 DOI: 10.1111/rda.70223

Ashmita Debnath, Hemanth Kumar Pundla, Kaivalya Deshmukh, Vineet Kumar Pandey, Neeraj Srivastava, Dharmesh Goswami, Subrata Kumar Ghosh, S K Singh, Bhanu Prakash, Sujatha Lakshmi Narayanan, Ajoy Das, Praveen Singh

{"title":"Emerging Proteomic Approaches in Sperm Sorting: Shaping the Future of Reproductive Biotechnology.","authors":"Ashmita Debnath, Hemanth Kumar Pundla, Kaivalya Deshmukh, Vineet Kumar Pandey, Neeraj Srivastava, Dharmesh Goswami, Subrata Kumar Ghosh, S K Singh, Bhanu Prakash, Sujatha Lakshmi Narayanan, Ajoy Das, Praveen Singh","doi":"10.1111/rda.70223","DOIUrl":"10.1111/rda.70223","url":null,"abstract":"Sperm sexing is a prominent Assisted Reproductive Technology (ART) that enhances reproductive efficiency, boosts production and improves the economic viability of dairy farms. To meet the growing demand for milk and dairy products, improvements in genetic selection and breeding strategies are imperative. Towards this goal, the use of sex-sorted semen enables the preferential production of female calves, thereby directly contributing to faster genetic gain, increased milk yield, and an optimised herd structure. At present, the commercially available and widely adopted sperm sexing technique is flow cytometry, offering up to 90% sorting accuracy based on the difference in DNA content. However, its widespread application is limited due to high operational costs, technical complexity, and potential adverse effects on sperm viability and fertility. Recent advancements in proteomics and immunological methods have opened new avenues for developing cost-effective, non-invasive sperm-sorting technologies. Such approaches are designed to enhance sorting efficiency, maintain sperm integrity, and offer methods that can be effectively implemented on a larger scale to meet breeders' requirements. This review discusses the current status, technological limitations, regulatory frameworks, and future prospects of proteomic and immune-based strategies in bovine sperm sexing, with a focus on their potential to transform flexible bovine productivity at large.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70223"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147949447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary Protein Imbalance Impairs Sperm Morphology and Promotes Testicular Degeneration in Rams. 饲粮蛋白质失衡损害公羊精子形态，促进睾丸变性。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-05-01 DOI: 10.1111/rda.70215

Edson Ramos de Siqueira Filho, Frederico Ozanam Papa, Pedro Henrique de Oliveira Baldini, Andrey Osvaldo Souza Ferro, Karina Alberti, Tiago Troncarellli, Gabriel Augusto Monteiro

{"title":"Dietary Protein Imbalance Impairs Sperm Morphology and Promotes Testicular Degeneration in Rams.","authors":"Edson Ramos de Siqueira Filho, Frederico Ozanam Papa, Pedro Henrique de Oliveira Baldini, Andrey Osvaldo Souza Ferro, Karina Alberti, Tiago Troncarellli, Gabriel Augusto Monteiro","doi":"10.1111/rda.70215","DOIUrl":"10.1111/rda.70215","url":null,"abstract":"Reproductive efficiency is a primary determinant of profitability in ruminant production, with male fertility being a critical factor for genetic progress and herd productivity. While nutritional management is a central component in modulating reproductive capacity, the effects of excessive dietary protein on testicular function remain incompletely elucidated. This study evaluated the impact of different dietary crude protein (CP) levels on reproductive and metabolic parameters in rams. Twenty-eight Santa Inês rams were randomly assigned to four groups (n = 7) and fed for 120 days with isoenergetic diets containing distinct CP levels: control (13.4%), hypoproteic (11.4%), hyperproteic I (17.5%) and hyperproteic II (22.4%). Periodic evaluations of body parameters, sperm kinetics and morphology, testicular ultrasonography, testicular cytology and hormonal profiles were conducted. Diets did not influence body weight or sperm motility (p > 0.05). However, variations in testicular echogenicity were observed (p < 0.05), and rams fed hyperproteic diets showed a progressive and marked increase in total sperm defects, exceeding 29.5% on day 80 (p < 0.05) compared with the control group (12.86%). Cytological analysis confirmed the presence of multinucleated giant cells and testicular degenerative changes from day 40 onwards in the hyperproteic groups. In conclusion, diets containing 17.5% and 22.4% CP are detrimental to sperm morphology and testicular integrity in rams, even in the absence of changes in body weight or detectable ultrasonographic alterations. The diet with 13.4% CP proved more suitable for maintaining testicular function and reproductive health.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70215"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147820142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the Use of Recombinant Gonadotropins for Reproductive Biotechnologies in Ruminants: A Review. 重组促性腺激素在反刍动物生殖生物技术中的应用进展

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-05-01 DOI: 10.1111/rda.70216

Vitória Leite Di Domenico, Fabiane Pereira de Moraes, Natália Ávila de Castro, Fernando Caetano de Oliveira, Diogo Magnabosco, Bernardo Garziera Gasperin

{"title":"Advances in the Use of Recombinant Gonadotropins for Reproductive Biotechnologies in Ruminants: A Review.","authors":"Vitória Leite Di Domenico, Fabiane Pereira de Moraes, Natália Ávila de Castro, Fernando Caetano de Oliveira, Diogo Magnabosco, Bernardo Garziera Gasperin","doi":"10.1111/rda.70216","DOIUrl":"10.1111/rda.70216","url":null,"abstract":"Recombinant gonadotropins, particularly recombinant follicle-stimulating hormone (rFSH) and recombinant equine chorionic gonadotropin (reCG), have emerged as viable alternatives to traditional animal-derived hormones in reproductive biotechnologies. This review aims to provide an updated and comprehensive overview of recombinant gonadotropins, with emphasis on the most recent scientific and technological advances in production and their application. This research integrates studies published following the recent commercial introduction of these molecules and incorporates findings generated by our research group. A total of 12 field studies in ruminants met the inclusion criteria, comprising four evaluating rFSH for superovulation and eight assessing reCG across different reproductive applications. Recent studies demonstrate that rFSH achieves similar superovulatory outcomes to conventional protocols, while reCG effectively promotes follicular growth, ovulation, and fertility in cattle and sheep, with results comparable to purified eCG. Beyond reproductive efficacy, recombinant hormones offer advantages such as reduced animal use, consistent product quality, and simplified management. These findings highlight recombinant gonadotropins as promising, ethical, and efficient tools for advancing assisted reproduction in ruminants.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70216"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13175135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0